Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 274 |
| AccBSI | CCGCTC | 1 cut(s) | 71 |
| AccII | CGCG | 1 cut(s) | 139 |
| AciI | CCGC | 6 cut(s) | 55, 71, 121, 266, 353, 483 |
| AclWI | GGATC | 4 cut(s) | 80, 94, 107, 343 |
| AcuI | CTGAAG | 1 cut(s) | 214 |
| AfaI | GTAC | 1 cut(s) | 75 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 70, 299 |
| AgeI | ACCGGT | 1 cut(s) | 160 |
| AgsI | TTSAA | 2 cut(s) | 113, 310 |
| AluBI | AGCT | 3 cut(s) | 17, 397, 462 |
| AluI | AGCT | 3 cut(s) | 17, 397, 462 |
| Alw21I | GWGCWC | 1 cut(s) | 378 |
| AlwI | GGATC | 4 cut(s) | 80, 94, 107, 343 |
| AoxI | GGCC | 1 cut(s) | 295 |
| ApeKI | GCWGC | 2 cut(s) | 459, 462 |
| AsiGI | ACCGGT | 1 cut(s) | 160 |
| AspLEI | GCGC | 1 cut(s) | 208 |
| AspS9I | GGNCC | 1 cut(s) | 295 |
| AsuC2I | CCSGG | 2 cut(s) | 294, 333 |
| AsuHPI | GGTGA | 3 cut(s) | 150, 184, 314 |
| BaeGI | GKGCMC | 1 cut(s) | 279 |
| BamHI | GGATCC | 1 cut(s) | 99 |
| BanI | GGYRCC | 1 cut(s) | 274 |
| Bbv12I | GWGCWC | 1 cut(s) | 378 |
| BbvI | GCAGC | 2 cut(s) | 449, 471 |
| BccI | CCATC | 1 cut(s) | 446 |
| BcnI | CCSGG | 2 cut(s) | 294, 333 |
| BfaI | CTAG | 1 cut(s) | 320 |
| BfmI | CTRYAG | 1 cut(s) | 463 |
| BisI | GCNGC | 2 cut(s) | 460, 463 |
| BlsI | GCNGC | 2 cut(s) | 461, 464 |
| Bme1390I | CCNGG | 2 cut(s) | 294, 333 |
| BmgT120I | GGNCC | 1 cut(s) | 295 |
| BmiI | GGNNCC | 4 cut(s) | 101, 276, 366, 387 |
| BmrFI | CCNGG | 2 cut(s) | 294, 333 |
| BpmI | CTGGAG | 1 cut(s) | 441 |
| BpuMI | CCSGG | 2 cut(s) | 294, 333 |
| BsaJI | CCNNGG | 4 cut(s) | 64, 235, 298, 313 |
| BsaWI | WCCGGW | 1 cut(s) | 160 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 414, 444 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 70, 299 |
| Bse118I | RCCGGY | 2 cut(s) | 160, 479 |
| Bse3DI | GCAATG | 2 cut(s) | 294, 461 |
| BseDI | CCNNGG | 4 cut(s) | 64, 235, 298, 313 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 70, 299 |
| BseMI | GCAATG | 2 cut(s) | 294, 461 |
| BseMII | CTCAG | 1 cut(s) | 68 |
| BseRI | GAGGAG | 1 cut(s) | 284 |
| BseSI | GKGCMC | 1 cut(s) | 279 |
| BseXI | GCAGC | 2 cut(s) | 449, 471 |
| BseYI | CCCAGC | 1 cut(s) | 397 |
| BsgI | GTGCAG | 1 cut(s) | 312 |
| Bsh1236I | CGCG | 1 cut(s) | 139 |
| BshFI | GGCC | 1 cut(s) | 297 |
| BshNI | GGYRCC | 1 cut(s) | 274 |
| BshTI | ACCGGT | 1 cut(s) | 160 |
| BsiHKAI | GWGCWC | 1 cut(s) | 378 |
| BsiSI | CCGG | 5 cut(s) | 161, 293, 333, 368, 480 |
| BslFI | GGGAC | 1 cut(s) | 59 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 70, 299 |
| BsmFI | GGGAC | 1 cut(s) | 59 |
| BsnI | GGCC | 1 cut(s) | 297 |
| Bsp1286I | GDGCHC | 2 cut(s) | 279, 378 |
| Bsp143I | GATC | 3 cut(s) | 85, 99, 335 |
| Bsp19I | CCATGG | 1 cut(s) | 235 |
| Bsp68I | TCGCGA | 1 cut(s) | 139 |
| BspACI | CCGC | 6 cut(s) | 55, 71, 121, 266, 353, 483 |
| BspANI | GGCC | 1 cut(s) | 297 |
| BspCNI | CTCAG | 1 cut(s) | 69 |
| BspFNI | CGCG | 1 cut(s) | 139 |
| BspLI | GGNNCC | 4 cut(s) | 101, 276, 366, 387 |
| BspMAI | CTGCAG | 1 cut(s) | 467 |
| BspPI | GGATC | 4 cut(s) | 80, 94, 107, 343 |
| BspT107I | GGYRCC | 1 cut(s) | 274 |
| BsrBI | CCGCTC | 1 cut(s) | 71 |
| BsrDI | GCAATG | 2 cut(s) | 294, 461 |
| BsrFI | RCCGGY | 2 cut(s) | 160, 479 |
| BssAI | RCCGGY | 2 cut(s) | 160, 479 |
| BssECI | CCNNGG | 4 cut(s) | 64, 235, 298, 313 |
| BssMI | GATC | 3 cut(s) | 85, 99, 335 |
| BssT1I | CCWWGG | 3 cut(s) | 64, 235, 298 |
| Bst4CI | ACNGT | 3 cut(s) | 178, 314, 412 |
| BstC8I | GCNNGC | 1 cut(s) | 264 |
| BstDEI | CTNAG | 1 cut(s) | 77 |
| BstDSI | CCRYGG | 2 cut(s) | 235, 313 |
| BstFNI | CGCG | 1 cut(s) | 139 |
| BstHHI | GCGC | 1 cut(s) | 208 |
| BstKTI | GATC | 3 cut(s) | 88, 102, 338 |
| BstMBI | GATC | 3 cut(s) | 85, 99, 335 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 373, 425, 434 |
| BstSCI | CCNGG | 2 cut(s) | 292, 331 |
| BstSFI | CTRYAG | 1 cut(s) | 463 |
| BstSLI | GKGCMC | 1 cut(s) | 279 |
| BstUI | CGCG | 1 cut(s) | 139 |
| BstV1I | GCAGC | 2 cut(s) | 449, 471 |
| BstX2I | RGATCY | 1 cut(s) | 99 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 242 |
| BstYI | RGATCY | 1 cut(s) | 99 |
| BsuRI | GGCC | 1 cut(s) | 297 |
| BtgI | CCRYGG | 2 cut(s) | 235, 313 |
| BtuMI | TCGCGA | 1 cut(s) | 139 |
| Cac8I | GCNNGC | 1 cut(s) | 264 |
| CfoI | GCGC | 1 cut(s) | 208 |
| Cfr10I | RCCGGY | 2 cut(s) | 160, 479 |
| Cfr13I | GGNCC | 1 cut(s) | 295 |
| Csp6I | GTAC | 1 cut(s) | 74 |
| CspAI | ACCGGT | 1 cut(s) | 160 |
| CviAII | CATG | 2 cut(s) | 203, 236 |
| CviQI | GTAC | 1 cut(s) | 74 |
| DdeI | CTNAG | 1 cut(s) | 77 |
| DpnI | GATC | 3 cut(s) | 87, 101, 337 |
| DpnII | GATC | 3 cut(s) | 85, 99, 335 |
| EciI | GGCGGA | 2 cut(s) | 110, 281 |
| Eco130I | CCWWGG | 3 cut(s) | 64, 235, 298 |
| Eco57I | CTGAAG | 1 cut(s) | 214 |
| EcoT14I | CCWWGG | 3 cut(s) | 64, 235, 298 |
| ErhI | CCWWGG | 3 cut(s) | 64, 235, 298 |
| FaeI | CATG | 2 cut(s) | 206, 239 |
| FaiI | YATR | 3 cut(s) | 192, 204, 237 |
| FaqI | GGGAC | 1 cut(s) | 59 |
| FatI | CATG | 2 cut(s) | 202, 235 |
| FauI | CCCGC | 2 cut(s) | 48, 476 |
| Fnu4HI | GCNGC | 2 cut(s) | 460, 463 |
| Fsp4HI | GCNGC | 2 cut(s) | 460, 463 |
| FspBI | CTAG | 1 cut(s) | 320 |
| GlaI | GCGC | 1 cut(s) | 207 |
| GluI | GCNGC | 2 cut(s) | 460, 463 |
| GsaI | CCCAGC | 1 cut(s) | 401 |
| GsuI | CTGGAG | 1 cut(s) | 441 |
| HaeIII | GGCC | 1 cut(s) | 297 |
| HapII | CCGG | 5 cut(s) | 161, 293, 333, 368, 480 |
| HhaI | GCGC | 1 cut(s) | 208 |
| Hin1II | CATG | 2 cut(s) | 206, 239 |
| Hin6I | GCGC | 1 cut(s) | 206 |
| HinP1I | GCGC | 1 cut(s) | 206 |
| HincII | GTYRAC | 1 cut(s) | 61 |
| HindII | GTYRAC | 1 cut(s) | 61 |
| HinfI | GANTC | 2 cut(s) | 109, 323 |
| HpaII | CCGG | 5 cut(s) | 161, 293, 333, 368, 480 |
| HphI | GGTGA | 3 cut(s) | 150, 184, 314 |
| Hpy166II | GTNNAC | 1 cut(s) | 61 |
| Hpy188I | TCNGA | 4 cut(s) | 32, 340, 390, 472 |
| Hpy188III | TCNNGA | 4 cut(s) | 94, 113, 138, 320 |
| Hpy8I | GTNNAC | 1 cut(s) | 61 |
| Hpy99I | CGWCG | 1 cut(s) | 394 |
| HpyAV | CCTTC | 1 cut(s) | 120 |
| HpyCH4III | ACNGT | 3 cut(s) | 178, 314, 412 |
| HpyCH4V | TGCA | 4 cut(s) | 187, 329, 459, 465 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 373, 425, 434 |
| HpyF3I | CTNAG | 1 cut(s) | 77 |
| Hsp92II | CATG | 2 cut(s) | 206, 239 |
| HspAI | GCGC | 1 cut(s) | 206 |
| Kzo9I | GATC | 3 cut(s) | 85, 99, 335 |
| LmnI | GCTCC | 4 cut(s) | 68, 364, 391, 394 |
| Lsp1109I | GCAGC | 2 cut(s) | 449, 471 |
| MaeI | CTAG | 1 cut(s) | 320 |
| MaeIII | GTNAC | 4 cut(s) | 172, 251, 302, 406 |
| MalI | GATC | 3 cut(s) | 87, 101, 337 |
| MbiI | CCGCTC | 1 cut(s) | 71 |
| MboI | GATC | 3 cut(s) | 85, 99, 335 |
| MboII | GAAGA | 1 cut(s) | 220 |
| MflI | RGATCY | 1 cut(s) | 99 |
| MhlI | GDGCHC | 2 cut(s) | 279, 378 |
| MlyI | GAGTC | 1 cut(s) | 317 |
| MmeI | TCCRAC | 1 cut(s) | 413 |
| MnlI | CCTC | 5 cut(s) | 90, 252, 262, 265, 364 |
| MseI | TTAA | 1 cut(s) | 381 |
| MslI | CAYNNNNRTG | 1 cut(s) | 240 |
| MspA1I | CMGCKG | 1 cut(s) | 462 |
| MspI | CCGG | 5 cut(s) | 161, 293, 333, 368, 480 |
| MspR9I | CCNGG | 2 cut(s) | 294, 333 |
| MvnI | CGCG | 1 cut(s) | 139 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 373, 425, 434 |
| NciI | CCSGG | 2 cut(s) | 294, 333 |
| NcoI | CCATGG | 1 cut(s) | 235 |
| NdeII | GATC | 3 cut(s) | 85, 99, 335 |
| NlaIII | CATG | 2 cut(s) | 206, 239 |
| NlaIV | GGNNCC | 4 cut(s) | 101, 276, 366, 387 |
| NmuCI | GTSAC | 4 cut(s) | 172, 251, 302, 406 |
| NruI | TCGCGA | 1 cut(s) | 139 |
| PfeI | GAWTC | 1 cut(s) | 109 |
| PinAI | ACCGGT | 1 cut(s) | 160 |
| PkrI | GCNGC | 2 cut(s) | 461, 464 |
| PleI | GAGTC | 1 cut(s) | 317 |
| PpsI | GAGTC | 1 cut(s) | 317 |
| PspFI | CCCAGC | 1 cut(s) | 397 |
| PspN4I | GGNNCC | 4 cut(s) | 101, 276, 366, 387 |
| PspPI | GGNCC | 1 cut(s) | 295 |
| PstI | CTGCAG | 1 cut(s) | 467 |
| PsuI | RGATCY | 1 cut(s) | 99 |
| PvuII | CAGCTG | 1 cut(s) | 462 |
| RruI | TCGCGA | 1 cut(s) | 139 |
| RsaI | GTAC | 1 cut(s) | 75 |
| RsaNI | GTAC | 1 cut(s) | 74 |
| RseI | CAYNNNNRTG | 1 cut(s) | 240 |
| SaqAI | TTAA | 1 cut(s) | 381 |
| SatI | GCNGC | 2 cut(s) | 460, 463 |
| Sau3AI | GATC | 3 cut(s) | 85, 99, 335 |
| Sau96I | GGNCC | 1 cut(s) | 295 |
| SchI | GAGTC | 1 cut(s) | 317 |
| ScrFI | CCNGG | 2 cut(s) | 294, 333 |
| SduI | GDGCHC | 2 cut(s) | 279, 378 |
| SetI | ASST | 6 cut(s) | 19, 276, 304, 375, 399, 464 |
| SfcI | CTRYAG | 1 cut(s) | 463 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 240 |
| SsiI | CCGC | 6 cut(s) | 55, 71, 121, 266, 353, 483 |
| SspMI | CTAG | 1 cut(s) | 320 |
| StyD4I | CCNGG | 2 cut(s) | 292, 331 |
| StyI | CCWWGG | 3 cut(s) | 64, 235, 298 |
| TaaI | ACNGT | 3 cut(s) | 178, 314, 412 |
| TfiI | GAWTC | 1 cut(s) | 109 |
| Tru1I | TTAA | 1 cut(s) | 381 |
| Tru9I | TTAA | 1 cut(s) | 381 |
| TseFI | GTSAC | 4 cut(s) | 172, 251, 302, 406 |
| TseI | GCWGC | 2 cut(s) | 459, 462 |
| Tsp45I | GTSAC | 4 cut(s) | 172, 251, 302, 406 |
| TspGWI | ACGGA | 2 cut(s) | 92, 407 |
| XbaI | TCTAGA | 1 cut(s) | 319 |
| XspI | CTAG | 1 cut(s) | 320 |