Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000051

Physical Location & Seq

Genomic Coordinates Reverse (-)

3694500 .. 3701603

Gene Structure 7104 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1048100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2589 bp

ATGGGTACCGTCAAGAAACCTTGTATGGTCTTTTTATGCTTTCTCTTCCTCTTGTCATGGATTTTCAATGTGGCGGCGGTTGCGGCGGTTGAAAACACAACTATCCAAGTGAATGTGGGTGTTGTTGTTGACCTCGATCAACCCAGTCAGAGCGGGAAGATGTATTTGAGTTGCATCAAAATGGCCATCGAGGACTTCTATGCATCTCATGCTCACTTCAAGACCAGGCTTGTTTTGAACACTAGGGACTCCAAGCAATCTGTTGTTGGTGCAGCTGCTGCAGACCTGATAAAGAATGTAGAAGTGCAAGCAATCCTGGGGCCAGTGACATCAATGCAGGCTAGCTTTGTTGTTAATCTTGGAGACCAAGCTCATGTGCCCATTTTATCATTTTCTGCAACAAGCCCCTCTCTTACTTCACTCCAAAGCTCTTACTTCTTCCGAATTACACAAACCGACTCTCAGCAAGTGAAAGCCATAAGTGCCATTGTTAAAAATTTCGGATGGAGACAAGTTGTGCCAATTTATGTAGACAATACGTACGGCGAGGGAGTCATACCCTTTTTAATTGATGCATTGCAAGAGGTTGATGCTCATGTCCCTTATCGGAGTGTCATTCCCGCATCACCTACAGATGTACAAATTGAAAAAGAGCTTCACAAGTTAATGACAATGCAAACTAGAGTCTTCATTGTGCACATGTTGCCCATACTTTGCACTAAGCTATTTGCCAAGGCAAAAGAGGTCGGAATGATGAGAAAAGGCTATGTTTGGATCATGACCAACGGGGTAGGAAATCGTTTATGGTCAATCCCTCCGGTAGTTCTGAATTCAATGCAAGGGGTATTAGGTGTGGAAACTGACGTTCCAAGAACAGAGGAGCTTGAAAATTTCAAAATGCGGTGGAAAAGACGATTCCAACAAGACAATCCAGCCATCATCGATGTTGATGTCGATGTATTTGGATTTCGGGCTTATGATGCAGTTTTTGCACTAGCCTTTGCAGTAGAAGAAGTTGGGTTTAATACAAACTCTGATTTTCAAAATAGGAATGATTCCGTTAACTCAACAGATCTTGATACGTTTGAGGTCTCCCAATATGGTCTAAAACTTTCACAAGCCATATCTAATACTTCATTCAAAGGTATAGCTGGTGACTTCAGGCTTGACGATGGGCAACTTCACGCATCGAATTTTAAGATAGTTAACGTAAATGGTGATGGAGTAAGAACTATTGCATTTTGGACACCAGAAAATGGTATGTTAAAGACACTGAATTCAGCAAACACAAGCACACTTTCAACTTCAACTTCCAAGTGCAATCTTACAAGCATACTTTCAACTTCCAAGTGCAATCTTTCACCGATTATATGGCCTGGAGATTCTTTCTCTGTTCCCAAGGGGTGGGAGATCCCAACAAATGACAAGAAGCTGAGAATTGGAGTTCCTGTAAAGCTTGGTTTTAGTGAGTTTGTTAAGGTAACCAAGAACCCTAGCACTAACACAACGCAAGTCACTGGGTTCAGTATTGATGTCTTTGAGGCTGCAGTTGAAGTACTACCATATGCACTTCCTTACGAGTTCATTCCCTTTGAGAATTCTGATGGAACAATGGCTGGCACGTACAATGATTTGGTCTATCAAGTATATCTTGAGAAATTTGATGCTGTGGTGGGAGATACCACGATTAGGGCAAACAGATCTTTGTACGTGGACTTTACGATGCCCTACACAGAATCGGGGGTAGTAATGGTTGTGCCAATCAGAGACACCAGGAGCAAAAATGCATGGGTTTTCTTGAAGCCCTTGACATGGGACTTGTGGCTTACGACTTTTTGTTTCTTCATCTTCATTGGTTTCGTCATCTGGGTACTTGAACATCAAATTAATGAAGACTTTCGCGGCCCTCCCTCACATCAAGTTGGCACAAGCTTTTGGTTCTCCTTCTCCACCATGGTGTTTTCACATATGGTTAGCAACTTGGCCAGATTCTTGATGATTATATGGGTGTTTGTTGTGCTAGTTTTGACACAAAGCTACACAGCTAATCTAGCATCGCTATTAACAGTTGAACAACTCCAACCAACTGTCACGAATATAAAGGATATACTAAGAAAAGGGGAGAATGTTGGATGCATGGAGAATAGCTTTGTTTGCGAACTCTTGATGAAACAAGTCGGTTTCCATGAGTCCAAGCTTAAGGCTATCAAAACCATGGAAGAATGTGATGAAGCTCTTTCGAAAGGGAGCGGAAAGGGTGGTATTGCAGCTGTTGTTGCTGGAACCCCATATATGGAGCTTTTCATTGCGAAATATTGTTCCAAATATACCATGATTGGTCCAATCTACAAAACTGATGGCTTTGGTTTTGTGTTTCCAAAGCGTTCTCCTCTTGTGCCCGATATTTCACAAGCAGTCCTAAGCGTGATGGAAGGAGAGAAGATCATGAAAATTGAGAGCAAATGGTTCAGTCAAGAAAGCAATTGTGAAGATAACTCCAGGACTCCAAGGATCTCAAGCAACAGCCTTGGTCTTGAAAGCTTTTGGGGCCTATTCCTAATTGCCGGGGCATGTCTTGGAGCAGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

863

Amino Acids

95.87

Weight (kDa)

5.89

Isoelectric Point (pI)

30.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	36 - 405	5e-23	Periplasmic binding protein
ANF_receptor	PF01094	58 - 407	6.3e-79	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	495 - 586	7.8e-08	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	501 - 824	6.5e-09	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	604 - 857	5.2e-38	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24710 AT2G24710 AT2G24710 AT2G24720 AT2G29100 AT2G29100 AT2G29110 AT2G29110 AT2G29120 AT5G27100
fragaria_vesca	FvH4_2g11260 FvH4_2g11280 FvH4_2g11310 FvH4_2g11310 FvH4_2g11331 FvH4_2g11331 FvH4_2g11340 FvH4_2g11340 FvH4_2g11340 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31990 FvH4_2g37140 FvH4_2g37150 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37161 FvH4_2g37161 FvH4_2g37163 FvH4_2g37163 FvH4_2g37170 FvH4_2g37181 FvH4_2g37191 FvH4_3g22781 FvH4_3g22781 FvH4_3g22781 FvH4_3g22790 FvH4_3g22790 FvH4_3g22810 FvH4_5g13460 FvH4_5g34751 FvH4_7g09130 FvH4_7g27991 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010
malus_domestica	MD08G1047600.v1.1 MD08G1047700.v1.1 MD08G1048100.v1.1 MD08G1048200.v1.1 MD08G1048300.v1.1 MD08G1048500.v1.1 MD08G1048600.v1.1 MD08G1048700.v1.1 MD08G1048900.v1.1 MD08G1049000.v1.1 MD14G1064700.v1.1 MD15G1045900.v1.1 MD15G1046000.v1.1 MD15G1046200.v1.1 MD15G1046400.v1.1 MD15G1059200.v1.1 MD15G1059500.v1.1 MD15G1059600.v1.1 MD15G1059700.v1.1
prunus_persica	Prupe.1G399500_v2.0.a1 Prupe.1G399600_v2.0.a1 Prupe.1G399700_v2.0.a1 Prupe.1G399800_v2.0.a1 Prupe.1G399900_v2.0.a1 Prupe.1G400000_v2.0.a1 Prupe.1G400300_v2.0.a1 Prupe.1G400400_v2.0.a1 Prupe.1G400500_v2.0.a1 Prupe.1G400600_v2.0.a1 Prupe.1G400700_v2.0.a1 Prupe.1G412700_v2.0.a1 Prupe.1G412800_v2.0.a1
pyrus_communis	pycom02g01350 pycom08g04020 pycom08g04040 pycom08g04080 pycom08g04090 pycom08g04100 pycom14g05240 pycom15g04300 pycom15g04310 pycom15g04330 pycom15g04340 pycom15g04380 pycom15g05560 pycom15g05570 pycom15g05580 pycom15g05600
rosa_chinensis	RchiOBHm_Chr1g0345071 RchiOBHm_Chr1g0345081 RchiOBHm_Chr1g0345171 RchiOBHm_Chr3g0480811 RchiOBHm_Chr3g0492581 RchiOBHm_Chr5g0020871 RchiOBHm_Chr5g0039801 RchiOBHm_Chr5g0039811 RchiOBHm_Chr5g0039861 RchiOBHm_Chr5g0039911 RchiOBHm_Chr5g0039941 RchiOBHm_Chr5g0039951 RchiOBHm_Chr5g0040011 RchiOBHm_Chr6g0269601 RchiOBHm_Chr6g0269621 RchiOBHm_Chr6g0269631 RchiOBHm_Chr6g0269641 RchiOBHm_Chr6g0269671 RchiOBHm_Chr6g0269681 RchiOBHm_Chr6g0269731 RchiOBHm_Chr6g0269751 RchiOBHm_Chr6g0269761 RchiOBHm_Chr6g0269771 RchiOBHm_Chr6g0269781 RchiOBHm_Chr6g0269861 RchiOBHm_Chr6g0269871 RchiOBHm_Chr6g0269881 RchiOBHm_Chr6g0269911 RchiOBHm_Chr6g0269921 RchiOBHm_Chr6g0269941 RchiOBHm_Chr6g0269951 RchiOBHm_Chr6g0304511 RchiOBHm_Chr6g0304621 RchiOBHm_Chr6g0304641 RchiOBHm_Chr6g0304651 RchiOBHm_Chr6g0304661 RchiOBHm_Chr6g0310781 RchiOBHm_Chr6g0310801 RchiOBHm_Chr6g0310811 RchiOBHm_Chr6g0310821 RchiOBHm_Chr6g0310831 RchiOBHm_Chr7g0183621 RchiOBHm_Chr7g0183651
rosa_laevigata	RLG00000005035 RLG00000005036 RLG00000010458 RLG00000010459 RLG00000010460 RLG00000010961 RLG00000010962 RLG00000010963 RLG00000010964 RLG00000010966 RLG00000010970 RLG00000013824 RLG00000013825 RLG00000013826 RLG00000013828 RLG00000013829 RLG00000013831 RLG00000013833 RLG00000013835 RLG00000013837 RLG00000013840 RLG00000013842 RLG00000013844 RLG00000022758 RLG00000023449 RLG00000026705 RLG00000028849 RLG00000028853 RLG00000028854 RLG00000032569 RLG00000033944 RLG00000033945 RLG00000033947 RLG00000033949 RLG00000033951 RLG00000034239
rosa_multiflora	Rmu_co8287891.1_g000001 Rmu_co8428745.1_g000001 Rmu_co8521767.1_g000002 Rmu_sc0000103.1_g000022 Rmu_sc0000103.1_g000031 Rmu_sc0000103.1_g000041 Rmu_sc0000103.1_g000053 Rmu_sc0000247.1_g000028 Rmu_sc0000247.1_g000051 Rmu_sc0000893.1_g000003 Rmu_sc0000893.1_g000017 Rmu_sc0001143.1_g000008 Rmu_sc0001143.1_g000019 Rmu_sc0001143.1_g000023 Rmu_sc0001975.1_g000003 Rmu_sc0001975.1_g000004 Rmu_sc0001975.1_g000005 Rmu_sc0001975.1_g000012 Rmu_sc0002151.1_g000006 Rmu_sc0003610.1_g000021 Rmu_sc0003682.1_g000001 Rmu_sc0003863.1_g000004 Rmu_sc0003863.1_g000007 Rmu_sc0003863.1_g000013 Rmu_sc0004196.1_g000005 Rmu_sc0004515.1_g000013 Rmu_sc0004515.1_g000024 Rmu_sc0004688.1_g000001 Rmu_sc0004688.1_g000003 Rmu_sc0004688.1_g000004 Rmu_sc0004748.1_g000006 Rmu_sc0004838.1_g000016 Rmu_sc0004838.1_g000017 Rmu_sc0005532.1_g000006 Rmu_sc0005532.1_g000011 Rmu_sc0005950.1_g000004 Rmu_sc0006576.1_g000005 Rmu_sc0006576.1_g000021 Rmu_sc0008520.1_g000002 Rmu_sc0008520.1_g000004 Rmu_sc0009130.1_g000002 Rmu_sc0018719.1_g000001 Rmu_sc0027713.1_g000005 Rmu_sc0040804.1_g000001 Rmu_ssc0000218.1_g000059 Rmu_ssc0000218.1_g000062 Rmu_ssc0000218.1_g000068 Rmu_ssc0000395.1_g000008 Rmu_ssc0000395.1_g000009 Rmu_ssc0000395.1_g000010 Rmu_ssc0000395.1_g000011 Rmu_ssc0000395.1_g000013 Rmu_ssc0000395.1_g000014
rosa_roxburghii	Rroxscaffold_1G00041270 Rroxscaffold_1G00041290 Rroxscaffold_1G00041300 Rroxscaffold_1G00041360 Rroxscaffold_1G00041380 Rroxscaffold_1G00057500 Rroxscaffold_3G00270350 Rroxscaffold_4G00283160 Rroxscaffold_4G00309210 Rroxscaffold_4G00309240 Rroxscaffold_4G00309260 Rroxscaffold_4G00309290 Rroxscaffold_4G00309360 Rroxscaffold_6G00392210 Rroxscaffold_6G00401030 Rroxscaffold_7G00158260 Rroxscaffold_7G00163620 Rroxscaffold_7G00163630 Rroxscaffold_7G00163650 Rroxscaffold_7G00163680 Rroxscaffold_7G00198270 Rroxscaffold_7G00198280 Rroxscaffold_7G00198330 Rroxscaffold_7G00198340 Rroxscaffold_7G00198360 Rroxscaffold_7G00198390 Rroxscaffold_7G00198410 Rroxscaffold_7G00198450 Rroxscaffold_7G00198460 Rroxscaffold_7G00198490 Rroxscaffold_7G00198500 Rroxscaffold_7G00198510 Rroxscaffold_7G00198530 Rroxscaffold_7G00198550 Rroxscaffold_7G00198600
rosa_rugosa	Rorug01G0175900 Rorug01G0176200 Rorug03G0186700 Rorug03G0254500 Rorug05G0182700 Rorug05G0183000 Rorug05G0183000 Rorug06G0049000 Rorug06G0049500 Rorug06G0049600.1 Rorug06G0049700 Rorug06G0050000 Rorug06G0050000 Rorug06G0050100 Rorug06G0050300.1 Rorug06G0050400 Rorug06G0051100 Rorug06G0051200 Rorug06G0051300 Rorug06G0051500 Rorug06G0051600 Rorug06G0051700 Rorug06G0332600 Rorug06G0332700 Rorug06G0332700 Rorug06G0383100 Rorug06G0383200 Rorug06G0383300 Rorug06G0383400 Rorug06G0459100 Rorug06G0459200
rosa_samantha	Rh1BG160400 Rh1BG160500 Rh1BG160700 Rh3DG263900 Rh3DG338400 Rh5CG164400 Rh5CG164500 Rh5CG305500 Rh5CG305900 Rh5CG306000 Rh5DG280800 Rh5DG281300 Rh6AG445300 Rh6AG497000 Rh6BG174700 Rh6BG457200 Rh6CG168900 Rh6CG169800 Rh6CG512700 Rh6CG512900 Rh6DG034100 Rh6DG160500 Rh6DG160800 Rh6DG160900 Rh6DG161100 Rh6DG161400 Rh6DG161800 Rh6DG162000 Rh6DG162100 Rh6DG162200 Rh6DG444800 Rh6DG445000 Rh6DG445100 Rh6DG445200 Rh6DG445300 Rh6DG499100 Rh6DG499200 Rh6DG499300 Rh6DG499400 Rh6DG499500 Rh6DG499700 Rh7BG059800 Rh7BG060000 Rh7BG258500 Rh7BG258700
rosa_wichuraiana	Rw0G006180 Rw0G007950 Rw0G018490 Rw0G022090 Rw1G015970 Rw1G015990 Rw3G021330 Rw5G025120 Rw5G025140 Rw5G025150 Rw5G025160 Rw5G025180 Rw5G025830 Rw5G025850 Rw6G014550 Rw6G014570 Rw6G014580 Rw6G014600 Rw6G014620 Rw6G014650 Rw6G014670 Rw6G038650 Rw6G038670 Rw6G038680 Rw6G038700 Rw6G038710 Rw6G038720 Rw6G043280 Rw6G043290 Rw7G004960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	5
AccB1I	GGYRCC	1 cut(s)	5
AccB7I	CCANNNNNTGG	2 cut(s)	1256, 1404
AccBSI	CCGCTC	2 cut(s)	153, 2250
AccI	GTMKAC	1 cut(s)	531
AccII	CGCG	1 cut(s)	1902
AciI	CCGC	9 cut(s)	74, 77, 83, 86, 153, 621, 901, 1902, 2250
AclWI	GGATC	3 cut(s)	782, 1405, 2519
AcoI	YGGCCR	2 cut(s)	183, 1983
AcsI	RAATTY	7 cut(s)	496, 829, 889, 1192, 1276, 1597, 1658
AcuI	CTGAAG	1 cut(s)	1144
AfaI	GTAC	7 cut(s)	7, 542, 639, 1557, 1625, 1709, 1872
AfiI	CCNNNNNNNGG	5 cut(s)	1256, 1404, 1689, 1812, 2293
AflII	CTTAAG	1 cut(s)	2198
AflIII	ACRYGT	1 cut(s)	699
AjnI	CCWGG	5 cut(s)	224, 315, 1375, 1772, 2498
AleI	CACNNNNGTG	1 cut(s)	1955
Alw21I	GWGCWC	1 cut(s)	699
Alw26I	GTCTC	4 cut(s)	357, 502, 1096, 1761
Alw44I	GTGCAC	1 cut(s)	695
AlwI	GGATC	3 cut(s)	782, 1405, 2519
AlwNI	CAGNNNCTG	1 cut(s)	278
AoxI	GGCC	6 cut(s)	183, 320, 1373, 1903, 1983, 2548
ApaLI	GTGCAC	1 cut(s)	695
ApeKI	GCWGC	6 cut(s)	272, 275, 278, 1544, 2267, 2582
ApoI	RAATTY	7 cut(s)	496, 829, 889, 1192, 1276, 1597, 1658
AseI	ATTAAT	1 cut(s)	1887
Asp700I	GAANNNNTTC	1 cut(s)	1896
Asp718I	GGTACC	1 cut(s)	5
AspS9I	GGNCC	4 cut(s)	320, 1904, 2339, 2548
AsuC2I	CCSGG	1 cut(s)	2566
AsuHPI	GGTGA	4 cut(s)	618, 1166, 1229, 1353
AsuII	TTCGAA	1 cut(s)	2240
AsuNHI	GCTAGC	1 cut(s)	341
AvaII	GGWCC	1 cut(s)	2339
BaeGI	GKGCMC	3 cut(s)	381, 699, 2400
BalI	TGGCCA	2 cut(s)	185, 1985
BanI	GGYRCC	1 cut(s)	5
BbsI	GAAGAC	2 cut(s)	679, 1899
Bbv12I	GWGCWC	1 cut(s)	699
BbvI	GCAGC	5 cut(s)	262, 265, 284, 1531, 2279
BccI	CCATC	8 cut(s)	194, 498, 944, 1166, 1214, 1598, 2351, 2422
BceAI	ACGGC	1 cut(s)	559
BciT130I	CCWGG	5 cut(s)	226, 317, 1377, 1774, 2500
BcnI	CCSGG	1 cut(s)	2566
BcoDI	GTCTC	4 cut(s)	357, 502, 1096, 1761
BfaI	CTAG	7 cut(s)	243, 342, 681, 995, 1494, 2021, 2051
BfmI	CTRYAG	3 cut(s)	279, 630, 1545
BfrI	CTTAAG	1 cut(s)	2198
BglII	AGATCT	2 cut(s)	1072, 1700
BisI	GCNGC	9 cut(s)	75, 84, 273, 276, 279, 1545, 1903, 2268, 2583
BlsI	GCNGC	9 cut(s)	76, 85, 274, 277, 280, 1546, 1904, 2269, 2584
BmcAI	AGTACT	1 cut(s)	1557
Bme1390I	CCNGG	6 cut(s)	226, 317, 1377, 1774, 2500, 2566
Bme18I	GGWCC	1 cut(s)	2339
BmgT120I	GGNCC	4 cut(s)	320, 1904, 2339, 2548
BmiI	GGNNCC	4 cut(s)	7, 321, 2284, 2549
BmrFI	CCNGG	6 cut(s)	226, 317, 1377, 1774, 2500, 2566
BmrI	ACTGGG	2 cut(s)	138, 1527
BmtI	GCTAGC	1 cut(s)	345
BmuI	ACTGGG	2 cut(s)	138, 1527
BpiI	GAAGAC	2 cut(s)	679, 1899
BpmI	CTGGAG	2 cut(s)	1398, 2482
Bpu10I	CCTNAGC	1 cut(s)	2420
Bpu14I	TTCGAA	1 cut(s)	2240
BpuEI	CTTGAG	2 cut(s)	1673, 2500
BpuMI	CCSGG	1 cut(s)	2566
Bsa29I	ATCGAT	1 cut(s)	942
BsaAI	YACGTR	3 cut(s)	540, 1623, 1711
BsaI	GGTCTC	2 cut(s)	357, 1096
BsaJI	CCNNGG	8 cut(s)	316, 732, 1398, 1953, 2214, 2507, 2527, 2565
BsaWI	WCCGGW	1 cut(s)	817
BsaXI	ACNNNNNCTCC	2 cut(s)	1434, 1464
Bsc4I	CCNNNNNNNGG	5 cut(s)	1256, 1404, 1689, 1812, 2293
Bse1I	ACTGG	3 cut(s)	144, 323, 1522
Bse3DI	GCAATG	2 cut(s)	575, 2304
BseBI	CCWGG	5 cut(s)	226, 317, 1377, 1774, 2500
BseCI	ATCGAT	1 cut(s)	942
BseDI	CCNNGG	8 cut(s)	316, 732, 1398, 1953, 2214, 2507, 2527, 2565
BseGI	GGATG	2 cut(s)	509, 2138
BseLI	CCNNNNNNNGG	5 cut(s)	1256, 1404, 1689, 1812, 2293
BseMI	GCAATG	2 cut(s)	575, 2304
BseMII	CTCAG	2 cut(s)	476, 1424
BseNI	ACTGG	3 cut(s)	144, 323, 1522
BseRI	GAGGAG	2 cut(s)	893, 2379
BseSI	GKGCMC	3 cut(s)	381, 699, 2400
BseXI	GCAGC	5 cut(s)	262, 265, 284, 1531, 2279
BsgI	GTGCAG	1 cut(s)	291
Bsh1236I	CGCG	1 cut(s)	1902
BshFI	GGCC	6 cut(s)	185, 322, 1375, 1905, 1985, 2550
BshNI	GGYRCC	1 cut(s)	5
BshVI	ATCGAT	1 cut(s)	942
BsiHKAI	GWGCWC	1 cut(s)	699
BsiSI	CCGG	2 cut(s)	818, 2565
BsiWI	CGTACG	1 cut(s)	540
BslFI	GGGAC	3 cut(s)	260, 584, 1829
BslI	CCNNNNNNNGG	5 cut(s)	1256, 1404, 1689, 1812, 2293
BsmAI	GTCTC	4 cut(s)	357, 502, 1096, 1761
BsmFI	GGGAC	3 cut(s)	260, 584, 1829
BsnI	GGCC	6 cut(s)	185, 322, 1375, 1905, 1985, 2550
Bso31I	GGTCTC	2 cut(s)	357, 1096
Bsp119I	TTCGAA	1 cut(s)	2240
Bsp1286I	GDGCHC	3 cut(s)	381, 699, 2400
Bsp1407I	TGTACA	1 cut(s)	637
Bsp143I	GATC	7 cut(s)	136, 774, 1072, 1410, 1700, 2442, 2511
Bsp19I	CCATGG	2 cut(s)	1953, 2214
BspACI	CCGC	9 cut(s)	74, 77, 83, 86, 153, 621, 901, 1902, 2250
BspANI	GGCC	6 cut(s)	185, 322, 1375, 1905, 1985, 2550
BspCNI	CTCAG	2 cut(s)	475, 1425
BspDI	ATCGAT	1 cut(s)	942
BspFNI	CGCG	1 cut(s)	1902
BspHI	TCATGA	2 cut(s)	777, 2445
BspLI	GGNNCC	4 cut(s)	7, 321, 2284, 2549
BspMAI	CTGCAG	2 cut(s)	283, 1549
BspOI	GCTAGC	1 cut(s)	345
BspPI	GGATC	3 cut(s)	782, 1405, 2519
BspT104I	TTCGAA	1 cut(s)	2240
BspT107I	GGYRCC	1 cut(s)	5
BspTI	CTTAAG	1 cut(s)	2198
BspTNI	GGTCTC	2 cut(s)	357, 1096
BsrBI	CCGCTC	2 cut(s)	153, 2250
BsrDI	GCAATG	2 cut(s)	575, 2304
BsrGI	TGTACA	1 cut(s)	637
BsrI	ACTGG	3 cut(s)	144, 323, 1522
BssECI	CCNNGG	8 cut(s)	316, 732, 1398, 1953, 2214, 2507, 2527, 2565
BssMI	GATC	7 cut(s)	136, 774, 1072, 1410, 1700, 2442, 2511
BssT1I	CCWWGG	6 cut(s)	732, 1398, 1953, 2214, 2507, 2527
Bst2UI	CCWGG	5 cut(s)	226, 317, 1377, 1774, 2500
Bst4CI	ACNGT	3 cut(s)	10, 2068, 2089
Bst6I	CTCTTC	1 cut(s)	50
BstAFI	CTTAAG	1 cut(s)	2198
BstAPI	GCANNNNNTGC	4 cut(s)	209, 278, 703, 989
BstAUI	TGTACA	1 cut(s)	637
BstBAI	YACGTR	3 cut(s)	540, 1623, 1711
BstBI	TTCGAA	1 cut(s)	2240
BstC8I	GCNNGC	4 cut(s)	309, 339, 343, 1618
BstDEI	CTNAG	5 cut(s)	462, 720, 1433, 2111, 2420
BstDSI	CCRYGG	2 cut(s)	1953, 2214
BstEII	GGTNACC	1 cut(s)	1480
BstF5I	GGATG	2 cut(s)	509, 2138
BstFNI	CGCG	1 cut(s)	1902
BstKTI	GATC	7 cut(s)	139, 777, 1075, 1413, 1703, 2445, 2514
BstMAI	GTCTC	4 cut(s)	357, 502, 1096, 1761
BstMBI	GATC	7 cut(s)	136, 774, 1072, 1410, 1700, 2442, 2511
BstNI	CCWGG	5 cut(s)	226, 317, 1377, 1774, 2500
BstNSI	RCATGY	2 cut(s)	703, 2574
BstPI	GGTNACC	1 cut(s)	1480
BstSCI	CCNGG	6 cut(s)	224, 315, 1375, 1772, 2498, 2564
BstSFI	CTRYAG	3 cut(s)	279, 630, 1545
BstSLI	GKGCMC	3 cut(s)	381, 699, 2400
BstSNI	TACGTA	1 cut(s)	540
BstUI	CGCG	1 cut(s)	1902
BstV1I	GCAGC	5 cut(s)	262, 265, 284, 1531, 2279
BstV2I	GAAGAC	2 cut(s)	679, 1899
BstX2I	RGATCY	4 cut(s)	1072, 1410, 1700, 2511
BstYI	RGATCY	4 cut(s)	1072, 1410, 1700, 2511
Bsu15I	ATCGAT	1 cut(s)	942
BsuRI	GGCC	6 cut(s)	185, 322, 1375, 1905, 1985, 2550
BsuTUI	ATCGAT	1 cut(s)	942
BtgI	CCRYGG	2 cut(s)	1953, 2214
BtgZI	GCGATG	1 cut(s)	2040
BtsCI	GGATG	2 cut(s)	509, 2138
BtsIMutI	CAGTG	3 cut(s)	330, 1271, 1515
Cac8I	GCNNGC	4 cut(s)	309, 339, 343, 1618
CaiI	CAGNNNCTG	1 cut(s)	278
CciI	TCATGA	2 cut(s)	777, 2445
Cfr13I	GGNCC	4 cut(s)	320, 1904, 2339, 2548
ClaI	ATCGAT	1 cut(s)	942
Csp6I	GTAC	7 cut(s)	6, 541, 638, 1556, 1624, 1708, 1871
CviQI	GTAC	7 cut(s)	6, 541, 638, 1556, 1624, 1708, 1871
DdeI	CTNAG	5 cut(s)	462, 720, 1433, 2111, 2420
DpnI	GATC	7 cut(s)	138, 776, 1074, 1412, 1702, 2444, 2513
DpnII	GATC	7 cut(s)	136, 774, 1072, 1410, 1700, 2442, 2511
EaeI	YGGCCR	2 cut(s)	183, 1983
Eam1104I	CTCTTC	1 cut(s)	50
EarI	CTCTTC	1 cut(s)	50
Eco105I	TACGTA	1 cut(s)	540
Eco130I	CCWWGG	6 cut(s)	732, 1398, 1953, 2214, 2507, 2527
Eco31I	GGTCTC	2 cut(s)	357, 1096
Eco47I	GGWCC	1 cut(s)	2339
Eco57I	CTGAAG	1 cut(s)	1144
Eco91I	GGTNACC	1 cut(s)	1480
EcoO109I	RGGNCCY	1 cut(s)	2548
EcoO65I	GGTNACC	1 cut(s)	1480
EcoRI	GAATTC	3 cut(s)	829, 1276, 1597
EcoRII	CCWGG	5 cut(s)	224, 315, 1375, 1772, 2498
EcoT14I	CCWWGG	6 cut(s)	732, 1398, 1953, 2214, 2507, 2527
EcoT22I	ATGCAT	4 cut(s)	205, 577, 1789, 2138
ErhI	CCWWGG	6 cut(s)	732, 1398, 1953, 2214, 2507, 2527
FalI	AAGNNNNNCTT	2 cut(s)	1635, 1667
FaqI	GGGAC	3 cut(s)	260, 584, 1829
FauI	CCCGC	2 cut(s)	146, 628
FauNDI	CATATG	2 cut(s)	1564, 1968
FblI	GTMKAC	1 cut(s)	531
Fnu4HI	GCNGC	9 cut(s)	75, 84, 273, 276, 279, 1545, 1903, 2268, 2583
FokI	GGATG	2 cut(s)	516, 2145
Fsp4HI	GCNGC	9 cut(s)	75, 84, 273, 276, 279, 1545, 1903, 2268, 2583
FspBI	CTAG	7 cut(s)	243, 342, 681, 995, 1494, 2021, 2051
GluI	GCNGC	9 cut(s)	75, 84, 273, 276, 279, 1545, 1903, 2268, 2583
GsuI	CTGGAG	2 cut(s)	1398, 2482
HaeIII	GGCC	6 cut(s)	185, 322, 1375, 1905, 1985, 2550
HapII	CCGG	2 cut(s)	818, 2565
HincII	GTYRAC	3 cut(s)	130, 1063, 1207
HindII	GTYRAC	3 cut(s)	130, 1063, 1207
HindIII	AAGCTT	4 cut(s)	1454, 1930, 2195, 2539
HpaI	GTTAAC	2 cut(s)	1063, 1207
HpaII	CCGG	2 cut(s)	818, 2565
HphI	GGTGA	4 cut(s)	618, 1166, 1229, 1353
Hpy166II	GTNNAC	6 cut(s)	130, 532, 697, 1063, 1207, 1714
Hpy188I	TCNGA	9 cut(s)	150, 443, 503, 609, 749, 828, 1036, 1603, 1766
Hpy8I	GTNNAC	6 cut(s)	130, 532, 697, 1063, 1207, 1714
HpyAV	CCTTC	2 cut(s)	1954, 2426
HpyCH4III	ACNGT	3 cut(s)	10, 2068, 2089
HpyCH4IV	ACGT	6 cut(s)	539, 864, 1082, 1209, 1622, 1710
HpyF3I	CTNAG	5 cut(s)	462, 720, 1433, 2111, 2420
HpySE526I	ACGT	6 cut(s)	539, 864, 1082, 1209, 1622, 1710
KpnI	GGTACC	1 cut(s)	9
KspAI	GTTAAC	2 cut(s)	1063, 1207
Kzo9I	GATC	7 cut(s)	136, 774, 1072, 1410, 1700, 2442, 2511
LmnI	GCTCC	5 cut(s)	880, 1776, 2247, 2296, 2579
Lsp1109I	GCAGC	5 cut(s)	262, 265, 284, 1531, 2279
MaeI	CTAG	7 cut(s)	243, 342, 681, 995, 1494, 2021, 2051
MaeII	ACGT	6 cut(s)	539, 864, 1082, 1209, 1622, 1710
MaeIII	GTNAC	5 cut(s)	325, 1154, 1480, 1513, 2089
MalI	GATC	7 cut(s)	138, 776, 1074, 1412, 1702, 2444, 2513
MbiI	CCGCTC	2 cut(s)	153, 2250
MboI	GATC	7 cut(s)	136, 774, 1072, 1410, 1700, 2442, 2511
MfeI	CAATTG	1 cut(s)	2482
MflI	RGATCY	4 cut(s)	1072, 1410, 1700, 2511
MhlI	GDGCHC	3 cut(s)	381, 699, 2400
MlsI	TGGCCA	2 cut(s)	185, 1985
MluNI	TGGCCA	2 cut(s)	185, 1985
MlyI	GAGTC	6 cut(s)	242, 452, 561, 693, 2198, 2497
MmeI	TCCRAC	4 cut(s)	727, 943, 2104, 2110
Mox20I	TGGCCA	2 cut(s)	185, 1985
Mph1103I	ATGCAT	4 cut(s)	205, 577, 1789, 2138
MroXI	GAANNNNTTC	1 cut(s)	1896
MscI	TGGCCA	2 cut(s)	185, 1985
MslI	CAYNNNNRTG	2 cut(s)	179, 1955
Msp20I	TGGCCA	2 cut(s)	185, 1985
MspA1I	CMGCKG	2 cut(s)	275, 2270
MspCI	CTTAAG	1 cut(s)	2198
MspI	CCGG	2 cut(s)	818, 2565
MspR9I	CCNGG	6 cut(s)	226, 317, 1377, 1774, 2500, 2566
MunI	CAATTG	1 cut(s)	2482
MvaI	CCWGG	5 cut(s)	226, 317, 1377, 1774, 2500
MvnI	CGCG	1 cut(s)	1902
NciI	CCSGG	1 cut(s)	2566
NcoI	CCATGG	2 cut(s)	1953, 2214
NdeI	CATATG	2 cut(s)	1564, 1968
NdeII	GATC	7 cut(s)	136, 774, 1072, 1410, 1700, 2442, 2511
NheI	GCTAGC	1 cut(s)	341
NlaIV	GGNNCC	4 cut(s)	7, 321, 2284, 2549
NmuCI	GTSAC	4 cut(s)	325, 1154, 1513, 2089
NsiI	ATGCAT	4 cut(s)	205, 577, 1789, 2138
NspI	RCATGY	2 cut(s)	703, 2574
NspV	TTCGAA	1 cut(s)	2240
OliI	CACNNNNGTG	1 cut(s)	1955
PagI	TCATGA	2 cut(s)	777, 2445
PciI	ACATGT	1 cut(s)	699
PdmI	GAANNNNTTC	1 cut(s)	1896
PfeI	GAWTC	5 cut(s)	915, 1055, 1382, 1736, 1989
Pfl23II	CGTACG	1 cut(s)	540
PflMI	CCANNNNNTGG	2 cut(s)	1256, 1404
PfoI	TCCNGGA	1 cut(s)	2498
PkrI	GCNGC	9 cut(s)	76, 85, 274, 277, 280, 1546, 1904, 2269, 2584
PleI	GAGTC	6 cut(s)	242, 452, 560, 692, 2197, 2497
PpsI	GAGTC	6 cut(s)	242, 452, 560, 692, 2197, 2497
Ppu21I	YACGTR	3 cut(s)	540, 1623, 1711
PscI	ACATGT	1 cut(s)	699
PshBI	ATTAAT	1 cut(s)	1887
Psp6I	CCWGG	5 cut(s)	224, 315, 1375, 1772, 2498
PspEI	GGTNACC	1 cut(s)	1480
PspGI	CCWGG	5 cut(s)	224, 315, 1375, 1772, 2498
PspLI	CGTACG	1 cut(s)	540
PspN4I	GGNNCC	4 cut(s)	7, 321, 2284, 2549
PspPI	GGNCC	4 cut(s)	320, 1904, 2339, 2548
PstI	CTGCAG	2 cut(s)	283, 1549
PstNI	CAGNNNCTG	1 cut(s)	278
PsuI	RGATCY	4 cut(s)	1072, 1410, 1700, 2511
PvuII	CAGCTG	2 cut(s)	275, 2270
RsaI	GTAC	7 cut(s)	7, 542, 639, 1557, 1625, 1709, 1872
RsaNI	GTAC	7 cut(s)	6, 541, 638, 1556, 1624, 1708, 1871
RseI	CAYNNNNRTG	2 cut(s)	179, 1955
SatI	GCNGC	9 cut(s)	75, 84, 273, 276, 279, 1545, 1903, 2268, 2583
Sau3AI	GATC	7 cut(s)	136, 774, 1072, 1410, 1700, 2442, 2511
Sau96I	GGNCC	4 cut(s)	320, 1904, 2339, 2548
ScaI	AGTACT	1 cut(s)	1557
SchI	GAGTC	6 cut(s)	242, 452, 561, 693, 2198, 2497
ScrFI	CCNGG	6 cut(s)	226, 317, 1377, 1774, 2500, 2566
SduI	GDGCHC	3 cut(s)	381, 699, 2400
SfcI	CTRYAG	3 cut(s)	279, 630, 1545
SfuI	TTCGAA	1 cut(s)	2240
SinI	GGWCC	1 cut(s)	2339
SmiMI	CAYNNNNRTG	2 cut(s)	179, 1955
SmlI	CTYRAG	3 cut(s)	1652, 2198, 2515
SmoI	CTYRAG	3 cut(s)	1652, 2198, 2515
SnaBI	TACGTA	1 cut(s)	540
SsiI	CCGC	9 cut(s)	74, 77, 83, 86, 153, 621, 901, 1902, 2250
SspI	AATATT	1 cut(s)	2315
SspMI	CTAG	7 cut(s)	243, 342, 681, 995, 1494, 2021, 2051
StyD4I	CCNGG	6 cut(s)	224, 315, 1375, 1772, 2498, 2564
StyI	CCWWGG	6 cut(s)	732, 1398, 1953, 2214, 2507, 2527
TaaI	ACNGT	3 cut(s)	10, 2068, 2089
TaiI	ACGT	6 cut(s)	542, 867, 1085, 1212, 1625, 1713
TaqI	TCGA	6 cut(s)	135, 189, 942, 954, 1190, 2240
TatI	WGTACW	2 cut(s)	637, 1555
TauI	GCSGC	3 cut(s)	77, 86, 1905
TfiI	GAWTC	5 cut(s)	915, 1055, 1382, 1736, 1989
TscAI	CASTG	3 cut(s)	330, 1278, 1522
TseFI	GTSAC	4 cut(s)	325, 1154, 1513, 2089
TseI	GCWGC	6 cut(s)	272, 275, 278, 1544, 2267, 2582
Tsp45I	GTSAC	4 cut(s)	325, 1154, 1513, 2089
TspGWI	ACGGA	1 cut(s)	1048
TspRI	CASTG	3 cut(s)	330, 1278, 1522
Van91I	CCANNNNNTGG	2 cut(s)	1256, 1404
Vha464I	CTTAAG	1 cut(s)	2198
VneI	GTGCAC	1 cut(s)	695
VpaK11BI	GGWCC	1 cut(s)	2339
VspI	ATTAAT	1 cut(s)	1887
XapI	RAATTY	7 cut(s)	496, 829, 889, 1192, 1276, 1597, 1658
XceI	RCATGY	2 cut(s)	703, 2574
XmiI	GTMKAC	1 cut(s)	531
XmnI	GAANNNNTTC	1 cut(s)	1896
XspI	CTAG	7 cut(s)	243, 342, 681, 995, 1494, 2021, 2051
ZrmI	AGTACT	1 cut(s)	1557
Zsp2I	ATGCAT	4 cut(s)	205, 577, 1789, 2138

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.