Rw6G014580

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000051
Physical Location & Seq
Forward (+)
26929598 .. 26933965
4368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014580.1

Sequence Viewer

Length: 2871 bp
ATGAAGAAGAAGATGAGTACATCAACCCTCGTCTTTTCTTCCTTAATCGTCTTCTCTCTATCGTCAAGTATTTTGTTGGTGATGGGACAGAAGACGAGTGTCTCAGTGAATGTTGGTGTGATTCTTGATGACCTTGAGTCTGTAAGTTCAAAAGTTAGGCTGAGCTGCATCGAATCGGCCATCTCAGACTTTTATAGCTCTCGTGGAAACACTCAAACGAGGCTGGTTCTGAGCATTAGGGACTCCAGAGGTGATGTTGTCGATGCCGCTGCTGCAGCTTTGGACCTAATAAAGAATGTTCAAGTGCAAGCAATCTTGGGACCGAAATCATCAATGCAAACCAACTTTGTAATTGATCTTGGAGGAAAAGCACGAGTTCCTATAATCTCATTTTCAGCAACAAGTCCTTCCCTCACTTCCCTTCCAAGTACATATTTTTTCCGAGCTGCACAGATTGATTCCCCTCAAGTCAAAGCCGTCGGGGCAATTATCAAAGCTTTTGGATGGAGAGAAGCTGTGCCAATCTATATTGACAATGAGTACGGCAACGGAGTATTACCATACTTGATCGACGCCTTGCAGATAGTTGAAGCTCATGTCCCTTATCGAAGTGCCATTTCTGGGTCAGCAACGGATGATCAAATTGAAAAGGAGCTTTTCAAGTTGATGACAATGCAAACTAGAGTCTTCATTGTGCACATGCCCGGCCCTCTAGGCTCTCGGCTTTTTGCCAAGGCACAAAAGCTAGGGATGATGAATGAAGGCTATGTATGGATCATGACAGATGGATTGAGCAATATTTTAAGTTCAACAAATGCTTCAGTCATCAGTTCAATGCAGGGCGCACTGGGGGTGAGGACCTATGTGCCGAAAACAAAAGAACTTGTCACTTTTAGAGAGCGATGGAAAAAGCAGTTCCACCAACAAAATCCAACCATCATTGATGTTGAACTAGATGTGTTTGCACTTTGGGCATATGATGCTGCTTATGCATTAGCCATGGCCGTTGAAGAACTTGGGACTACAAGCTTTGTCTTCCAAACCACCATTTCTTCCACAAACTCATCATCAGATCTAGAGACTTTGGGGGTATCGCAAAATGGTTATCAGCTTAGCCGATCCTTGGCAAGGACGAAATTTAGAGGTCTTGCTGGAAATTTCAGTTTTGATAATGGACAGTTACAATCTTCCATCTTTGAAATATTGAATGTGATTGGTGCTGGAGCAAGAGCAATTGGGTTTTGGACACCTGAAAATGGACTGGTGAGAAATCTGATCATTAATTCGACAAACACGTCTGCACCAAATTTAGGCTTTGTCATATGGCCCGGAGAGCATGGCTCCATTCCTAAGGGATGGGAGATCCCAACAAATGGAAAAAAGTTAAGAGTTGGAGTTCCAGTGAAGGATGGTTTTACAGAGTTTGTTAAGGTGGTACGTGATAACAGCACTAATACAACACCAGATGTTATTACTGGATTCTGTATTGACGTCTTTAATGCTGCAATGGAAGGATTACCATATGCCGTTACTTATGACTTCATTCCCTTTGCATATTCAAATGGCAGTTCTGCAGGAACTTATGATGATTTGGTCTATCAGGTTTTCCTTGGGGATTATGATGCAGTGGCAGCAGATTCAACAATCAGAGCAAACAGGTCACTGTATGTGGACTTTACATTGCCATATACTGAATCTGGTGTAGTAATGATAGTGCCAATGAAAGATAAAAACCGGATTAAAAATGCATGGATTTTCTTGAAGCCTTTGACATGGGACTTGTGGCTTGCGAGTTTCTTTTCCTTCGTCTTCATTGGTTTTGTGGTTTGGCTTCTTGAACACCGAGTAAATGAAGATTTTCGTGGGCCTCCCACACATCAAGTTGGCACTGGCCTCTGGTTCTCTTTCTCAACAATGGTTTTTGCACAGCGGGAGAGAGTCGTTAGCAACTTGTCTAGGTTTGTGGTGATAGTATGGGTATTTGTGGTGCTCATACTGACTCAAAGTTACACAGCGAGTCTGACTTCACTGCTAACTGTGCAAAAGCTTGAGCCAACTTATACAAATATAAGCCATCTGATAAGCAATACTAAGGAGTATGTGGGCTGCCTGGATGGCTCTTTTGTCTGCGACCTTTTACAACAACGTTTCCATGCTTCCAATATTAGGAAGTATAAATCTGCACAAGAATGTAACCAACTTTTGATAAACGGGAGTCAAAACGGGGGTATTGCTGCAGCCATTGACGAAACTCCAAACATGAAGCTTTTTCTTGCAAATTATTGCTCCAATTATACAATGATTGGTCCCATAATTAAAACTGATGGATTTGCCTTTGTTTTTCCTAAAGGTTCGCCTCTTGTACCAGATGTATCAAGGTCGATTCTGCAATTCACCGAGGGAGACTCGATGAAGGAACTTGAACGCAAATGGTTCGGGAACGAAACATCTTGTTCAGGTAGCAACCCCATGGTGTCTGATTCTAACAGTCTTAGCCTTGATAGGTTTTGGGGTCTGTTTCTGATTGCTGGCGTCACTTCGTTATTAGCCCTCATCATATTTGTAGCCTCATTTCTATGGAGTTATCAGCACAAAGGAATTAGGGGTATGCTCAAAGTGTTTGACTGTAAAGACCTGAGCTCCCATGCTTTTAAGAAGAAGAATTTGGGTGATCAGTTTGAGCGGTCACCAAGCTGCAGCAGCTACTCAATAAGCCCCTCGAATTTTAGCCATTCAAACCGTACAGATATTACAGAGATGGGGGAAGAAGCAATATTGCATGCTGGTCAAGTTGAATTTCCAGCTAGTGTGCTTGATCTTCCAAACTCAGCAATGCCAACAGCTCCTCAAAGTATTCAAGAGAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

956

Amino Acids

105.34

Weight (kDa)

6.36

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 37 - 342 7.9e-16 Periplasmic binding protein
ANF_receptor PF01094 55 - 406 7.5e-82 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 481 - 573 1.4e-06 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 491 - 813 3.8e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 591 - 845 1.3e-36 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24710 AT2G24710 AT2G24710 AT2G24720 AT2G29100 AT2G29100 AT2G29110 AT2G29110 AT2G29120 AT5G27100
fragaria_vesca FvH4_2g11260 FvH4_2g11280 FvH4_2g11310 FvH4_2g11310 FvH4_2g11331 FvH4_2g11331 FvH4_2g11340 FvH4_2g11340 FvH4_2g11340 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31990 FvH4_2g37140 FvH4_2g37150 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37161 FvH4_2g37161 FvH4_2g37163 FvH4_2g37163 FvH4_2g37170 FvH4_2g37181 FvH4_2g37191 FvH4_3g22781 FvH4_3g22781 FvH4_3g22781 FvH4_3g22790 FvH4_3g22790 FvH4_3g22810 FvH4_5g13460 FvH4_5g34751 FvH4_7g09130 FvH4_7g27991 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010
malus_domestica MD08G1047600.v1.1 MD08G1047700.v1.1 MD08G1048100.v1.1 MD08G1048200.v1.1 MD08G1048300.v1.1 MD08G1048500.v1.1 MD08G1048600.v1.1 MD08G1048700.v1.1 MD08G1048900.v1.1 MD08G1049000.v1.1 MD14G1064700.v1.1 MD15G1045900.v1.1 MD15G1046000.v1.1 MD15G1046200.v1.1 MD15G1046400.v1.1 MD15G1059200.v1.1 MD15G1059500.v1.1 MD15G1059600.v1.1 MD15G1059700.v1.1
prunus_persica Prupe.1G399500_v2.0.a1 Prupe.1G399600_v2.0.a1 Prupe.1G399700_v2.0.a1 Prupe.1G399800_v2.0.a1 Prupe.1G399900_v2.0.a1 Prupe.1G400000_v2.0.a1 Prupe.1G400300_v2.0.a1 Prupe.1G400400_v2.0.a1 Prupe.1G400500_v2.0.a1 Prupe.1G400600_v2.0.a1 Prupe.1G400700_v2.0.a1 Prupe.1G412700_v2.0.a1 Prupe.1G412800_v2.0.a1
pyrus_communis pycom02g01350 pycom08g04020 pycom08g04040 pycom08g04080 pycom08g04090 pycom08g04100 pycom14g05240 pycom15g04300 pycom15g04310 pycom15g04330 pycom15g04340 pycom15g04380 pycom15g05560 pycom15g05570 pycom15g05580 pycom15g05600
rosa_chinensis RchiOBHm_Chr1g0345071 RchiOBHm_Chr1g0345081 RchiOBHm_Chr1g0345171 RchiOBHm_Chr3g0480811 RchiOBHm_Chr3g0492581 RchiOBHm_Chr5g0020871 RchiOBHm_Chr5g0039801 RchiOBHm_Chr5g0039811 RchiOBHm_Chr5g0039861 RchiOBHm_Chr5g0039911 RchiOBHm_Chr5g0039941 RchiOBHm_Chr5g0039951 RchiOBHm_Chr5g0040011 RchiOBHm_Chr6g0269601 RchiOBHm_Chr6g0269621 RchiOBHm_Chr6g0269631 RchiOBHm_Chr6g0269641 RchiOBHm_Chr6g0269671 RchiOBHm_Chr6g0269681 RchiOBHm_Chr6g0269731 RchiOBHm_Chr6g0269751 RchiOBHm_Chr6g0269761 RchiOBHm_Chr6g0269771 RchiOBHm_Chr6g0269781 RchiOBHm_Chr6g0269861 RchiOBHm_Chr6g0269871 RchiOBHm_Chr6g0269881 RchiOBHm_Chr6g0269911 RchiOBHm_Chr6g0269921 RchiOBHm_Chr6g0269941 RchiOBHm_Chr6g0269951 RchiOBHm_Chr6g0304511 RchiOBHm_Chr6g0304621 RchiOBHm_Chr6g0304641 RchiOBHm_Chr6g0304651 RchiOBHm_Chr6g0304661 RchiOBHm_Chr6g0310781 RchiOBHm_Chr6g0310801 RchiOBHm_Chr6g0310811 RchiOBHm_Chr6g0310821 RchiOBHm_Chr6g0310831 RchiOBHm_Chr7g0183621 RchiOBHm_Chr7g0183651
rosa_laevigata RLG00000005035 RLG00000005036 RLG00000010458 RLG00000010459 RLG00000010460 RLG00000010961 RLG00000010962 RLG00000010963 RLG00000010964 RLG00000010966 RLG00000010970 RLG00000013824 RLG00000013825 RLG00000013826 RLG00000013828 RLG00000013829 RLG00000013831 RLG00000013833 RLG00000013835 RLG00000013837 RLG00000013840 RLG00000013842 RLG00000013844 RLG00000022758 RLG00000023449 RLG00000026705 RLG00000028849 RLG00000028853 RLG00000028854 RLG00000032569 RLG00000033944 RLG00000033945 RLG00000033947 RLG00000033949 RLG00000033951 RLG00000034239
rosa_multiflora Rmu_co8287891.1_g000001 Rmu_co8428745.1_g000001 Rmu_co8521767.1_g000002 Rmu_sc0000103.1_g000022 Rmu_sc0000103.1_g000031 Rmu_sc0000103.1_g000041 Rmu_sc0000103.1_g000053 Rmu_sc0000247.1_g000028 Rmu_sc0000247.1_g000051 Rmu_sc0000893.1_g000003 Rmu_sc0000893.1_g000017 Rmu_sc0001143.1_g000008 Rmu_sc0001143.1_g000019 Rmu_sc0001143.1_g000023 Rmu_sc0001975.1_g000003 Rmu_sc0001975.1_g000004 Rmu_sc0001975.1_g000005 Rmu_sc0001975.1_g000012 Rmu_sc0002151.1_g000006 Rmu_sc0003610.1_g000021 Rmu_sc0003682.1_g000001 Rmu_sc0003863.1_g000004 Rmu_sc0003863.1_g000007 Rmu_sc0003863.1_g000013 Rmu_sc0004196.1_g000005 Rmu_sc0004515.1_g000013 Rmu_sc0004515.1_g000024 Rmu_sc0004688.1_g000001 Rmu_sc0004688.1_g000003 Rmu_sc0004688.1_g000004 Rmu_sc0004748.1_g000006 Rmu_sc0004838.1_g000016 Rmu_sc0004838.1_g000017 Rmu_sc0005532.1_g000006 Rmu_sc0005532.1_g000011 Rmu_sc0005950.1_g000004 Rmu_sc0006576.1_g000005 Rmu_sc0006576.1_g000021 Rmu_sc0008520.1_g000002 Rmu_sc0008520.1_g000004 Rmu_sc0009130.1_g000002 Rmu_sc0018719.1_g000001 Rmu_sc0027713.1_g000005 Rmu_sc0040804.1_g000001 Rmu_ssc0000218.1_g000059 Rmu_ssc0000218.1_g000062 Rmu_ssc0000218.1_g000068 Rmu_ssc0000395.1_g000008 Rmu_ssc0000395.1_g000009 Rmu_ssc0000395.1_g000010 Rmu_ssc0000395.1_g000011 Rmu_ssc0000395.1_g000013 Rmu_ssc0000395.1_g000014
rosa_roxburghii Rroxscaffold_1G00041270 Rroxscaffold_1G00041290 Rroxscaffold_1G00041300 Rroxscaffold_1G00041360 Rroxscaffold_1G00041380 Rroxscaffold_1G00057500 Rroxscaffold_3G00270350 Rroxscaffold_4G00283160 Rroxscaffold_4G00309210 Rroxscaffold_4G00309240 Rroxscaffold_4G00309260 Rroxscaffold_4G00309290 Rroxscaffold_4G00309360 Rroxscaffold_6G00392210 Rroxscaffold_6G00401030 Rroxscaffold_7G00158260 Rroxscaffold_7G00163620 Rroxscaffold_7G00163630 Rroxscaffold_7G00163650 Rroxscaffold_7G00163680 Rroxscaffold_7G00198270 Rroxscaffold_7G00198280 Rroxscaffold_7G00198330 Rroxscaffold_7G00198340 Rroxscaffold_7G00198360 Rroxscaffold_7G00198390 Rroxscaffold_7G00198410 Rroxscaffold_7G00198450 Rroxscaffold_7G00198460 Rroxscaffold_7G00198490 Rroxscaffold_7G00198500 Rroxscaffold_7G00198510 Rroxscaffold_7G00198530 Rroxscaffold_7G00198550 Rroxscaffold_7G00198600
rosa_rugosa Rorug01G0175900 Rorug01G0176200 Rorug03G0186700 Rorug03G0254500 Rorug05G0182700 Rorug05G0183000 Rorug05G0183000 Rorug06G0049000 Rorug06G0049500 Rorug06G0049600.1 Rorug06G0049700 Rorug06G0050000 Rorug06G0050000 Rorug06G0050100 Rorug06G0050300.1 Rorug06G0050400 Rorug06G0051100 Rorug06G0051200 Rorug06G0051300 Rorug06G0051500 Rorug06G0051600 Rorug06G0051700 Rorug06G0332600 Rorug06G0332700 Rorug06G0332700 Rorug06G0383100 Rorug06G0383200 Rorug06G0383300 Rorug06G0383400 Rorug06G0459100 Rorug06G0459200
rosa_samantha Rh1BG160400 Rh1BG160500 Rh1BG160700 Rh3DG263900 Rh3DG338400 Rh5CG164400 Rh5CG164500 Rh5CG305500 Rh5CG305900 Rh5CG306000 Rh5DG280800 Rh5DG281300 Rh6AG445300 Rh6AG497000 Rh6BG174700 Rh6BG457200 Rh6CG168900 Rh6CG169800 Rh6CG512700 Rh6CG512900 Rh6DG034100 Rh6DG160500 Rh6DG160800 Rh6DG160900 Rh6DG161100 Rh6DG161400 Rh6DG161800 Rh6DG162000 Rh6DG162100 Rh6DG162200 Rh6DG444800 Rh6DG445000 Rh6DG445100 Rh6DG445200 Rh6DG445300 Rh6DG499100 Rh6DG499200 Rh6DG499300 Rh6DG499400 Rh6DG499500 Rh6DG499700 Rh7BG059800 Rh7BG060000 Rh7BG258500 Rh7BG258700
rosa_wichuraiana Rw0G006180 Rw0G007950 Rw0G018490 Rw0G022090 Rw1G015970 Rw1G015990 Rw3G021330 Rw5G025120 Rw5G025140 Rw5G025150 Rw5G025160 Rw5G025180 Rw5G025830 Rw5G025850 Rw6G014550 Rw6G014570 Rw6G014580 Rw6G014600 Rw6G014620 Rw6G014650 Rw6G014670 Rw6G038650 Rw6G038670 Rw6G038680 Rw6G038700 Rw6G038710 Rw6G038720 Rw6G043280 Rw6G043290 Rw7G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1294
AatII GACGTC 1 cut(s) 1494
AccB7I CCANNNNNTGG 1 cut(s) 1373
AccBSI CCGCTC 1 cut(s) 2683
AciI CCGC 3 cut(s) 267, 1930, 2683
AclI AACGTT 1 cut(s) 2146
AclWI GGATC 3 cut(s) 782, 1113, 1357
AcoI YGGCCR 2 cut(s) 177, 1002
AcsI RAATTY 6 cut(s) 1136, 1156, 1306, 2662, 2722, 2795
AcuI CTGAAG 1 cut(s) 804
AcyI GRCGYC 3 cut(s) 573, 1491, 2532
AfaI GTAC 6 cut(s) 19, 430, 542, 1437, 2364, 2743
AfiI CCNNNNNNNGG 7 cut(s) 621, 1123, 1128, 1256, 1310, 1373, 2166
AflIII ACRYGT 1 cut(s) 1293
AhdI GACNNNNNGTC 1 cut(s) 136
AjiI CACGTC 1 cut(s) 1296
AjnI CCWGG 1 cut(s) 2109
AjuI GAANNNNNNNTTGG 2 cut(s) 2648, 2680
Alw21I GWGCWC 3 cut(s) 699, 1992, 2642
Alw26I GTCTC 3 cut(s) 106, 1073, 2397
Alw44I GTGCAC 1 cut(s) 695
AlwI GGATC 3 cut(s) 782, 1113, 1357
AoxI GGCC 6 cut(s) 177, 706, 1002, 1325, 1865, 1891
ApaLI GTGCAC 1 cut(s) 695
ApoI RAATTY 6 cut(s) 1136, 1156, 1306, 2662, 2722, 2795
AseI ATTAAT 1 cut(s) 1281
Asp700I GAANNNNTTC 1 cut(s) 1857
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 7 cut(s) 283, 320, 707, 858, 1326, 1865, 2306
AsuC2I CCSGG 2 cut(s) 705, 1329
AsuHPI GGTGA 8 cut(s) 91, 263, 865, 1276, 1978, 2386, 2679, 2681
AvaII GGWCC 4 cut(s) 283, 320, 858, 2306
AxyI CCTNAGG 1 cut(s) 1350
BaeGI GKGCMC 1 cut(s) 699
BanII GRGCYC 1 cut(s) 2642
BauI CACGAG 2 cut(s) 201, 372
BbsI GAAGAC 5 cut(s) 43, 98, 679, 1027, 1801
Bbv12I GWGCWC 3 cut(s) 699, 1992, 2642
BceAI ACGGC 4 cut(s) 461, 559, 989, 1511
BcgI CGANNNNNNTGC 4 cut(s) 251, 285, 2416, 2450
BciT130I CCWGG 1 cut(s) 2111
BclI TGATCA 3 cut(s) 637, 1275, 2671
BcnI CCSGG 2 cut(s) 705, 1329
BcoDI GTCTC 3 cut(s) 106, 1073, 2397
BfaI CTAG 7 cut(s) 681, 713, 746, 953, 1076, 1956, 2805
BfmI CTRYAG 4 cut(s) 273, 1572, 2235, 2695
BglI GCCNNNNNGGC 3 cut(s) 482, 714, 2115
BglII AGATCT 1 cut(s) 1072
BlpI GCTNAGC 2 cut(s) 161, 1112
Bme1390I CCNGG 3 cut(s) 705, 1329, 2111
Bme18I GGWCC 4 cut(s) 283, 320, 858, 2306
BmeRI GACNNNNNGTC 1 cut(s) 136
BmgBI CACGTC 1 cut(s) 1296
BmgT120I GGNCC 7 cut(s) 283, 320, 707, 858, 1326, 1865, 2306
BmiI GGNNCC 3 cut(s) 321, 1342, 2308
BmrFI CCNGG 3 cut(s) 705, 1329, 2111
BmrI ACTGGG 1 cut(s) 857
BmsI GCATC 4 cut(s) 177, 253, 970, 1612
BmuI ACTGGG 1 cut(s) 857
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 5 cut(s) 43, 98, 679, 1027, 1801
BplI GAGNNNNNCTC 4 cut(s) 1325, 1357, 2390, 2422
BpmI CTGGAG 2 cut(s) 229, 1242
Bpu10I CCTNAGC 1 cut(s) 2636
Bpu1102I GCTNAGC 2 cut(s) 161, 1112
BpuEI CTTGAG 3 cut(s) 155, 450, 2069
BpuMI CCSGG 2 cut(s) 705, 1329
BsaAI YACGTR 1 cut(s) 1439
BsaHI GRCGYC 3 cut(s) 573, 1491, 2532
BsaJI CCNNGG 6 cut(s) 732, 999, 1122, 1609, 2397, 2469
BsaWI WCCGGW 1 cut(s) 1734
BsaXI ACNNNNNCTCC 4 cut(s) 1386, 1416, 2624, 2654
Bsc4I CCNNNNNNNGG 7 cut(s) 621, 1123, 1128, 1256, 1310, 1373, 2166
Bse1I ACTGG 5 cut(s) 852, 1266, 1400, 1480, 1894
Bse21I CCTNAGG 1 cut(s) 1350
Bse3DI GCAATG 3 cut(s) 1512, 1679, 2838
BseBI CCWGG 1 cut(s) 2111
BseDI CCNNGG 6 cut(s) 732, 999, 1122, 1609, 2397, 2469
BseGI GGATG 6 cut(s) 509, 640, 756, 1361, 1414, 2119
BseLI CCNNNNNNNGG 7 cut(s) 621, 1123, 1128, 1256, 1310, 1373, 2166
BseMI GCAATG 3 cut(s) 1512, 1679, 2838
BseMII CTCAG 6 cut(s) 117, 152, 198, 221, 2627, 2841
BseNI ACTGG 5 cut(s) 852, 1266, 1400, 1480, 1894
BseRI GAGGAG 1 cut(s) 2835
BseSI GKGCMC 1 cut(s) 699
BsgI GTGCAG 3 cut(s) 432, 1284, 2166
BshFI GGCC 6 cut(s) 179, 708, 1004, 1327, 1867, 1893
BsiHKAI GWGCWC 3 cut(s) 699, 1992, 2642
BsiSI CCGG 3 cut(s) 705, 1329, 1735
BslFI GGGAC 7 cut(s) 99, 254, 333, 584, 1033, 1790, 2292
BslI CCNNNNNNNGG 7 cut(s) 621, 1123, 1128, 1256, 1310, 1373, 2166
BsmAI GTCTC 3 cut(s) 106, 1073, 2397
BsmFI GGGAC 7 cut(s) 99, 254, 333, 584, 1033, 1790, 2292
BsnI GGCC 6 cut(s) 179, 708, 1004, 1327, 1867, 1893
Bsp1286I GDGCHC 3 cut(s) 699, 1992, 2642
Bsp1720I GCTNAGC 2 cut(s) 161, 1112
Bsp19I CCATGG 2 cut(s) 999, 2469
BspACI CCGC 3 cut(s) 267, 1930, 2683
BspANI GGCC 6 cut(s) 179, 708, 1004, 1327, 1867, 1893
BspCNI CTCAG 6 cut(s) 116, 153, 197, 222, 2628, 2840
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 3 cut(s) 321, 1342, 2308
BspMAI CTGCAG 4 cut(s) 277, 1576, 2239, 2699
BspPI GGATC 3 cut(s) 782, 1113, 1357
BsrBI CCGCTC 1 cut(s) 2683
BsrDI GCAATG 3 cut(s) 1512, 1679, 2838
BsrI ACTGG 5 cut(s) 852, 1266, 1400, 1480, 1894
BssECI CCNNGG 6 cut(s) 732, 999, 1122, 1609, 2397, 2469
BssNI GRCGYC 3 cut(s) 573, 1491, 2532
BssSI CACGAG 2 cut(s) 201, 372
BssT1I CCWWGG 5 cut(s) 732, 999, 1122, 1609, 2469
Bst2BI CACGAG 2 cut(s) 201, 372
Bst2UI CCWGG 1 cut(s) 2111
Bst4CI ACNGT 6 cut(s) 1179, 1665, 2038, 2489, 2627, 2741
BstACI GRCGYC 3 cut(s) 573, 1491, 2532
BstAPI GCANNNNNTGC 1 cut(s) 980
BstBAI YACGTR 1 cut(s) 1439
BstC8I GCNNGC 4 cut(s) 309, 1788, 2530, 2781
BstDSI CCRYGG 2 cut(s) 999, 2469
BstEII GGTNACC 1 cut(s) 2685
BstENI CCTNNNNNAGG 1 cut(s) 1126
BstF5I GGATG 6 cut(s) 509, 640, 756, 1361, 1414, 2119
BstHHI GCGC 1 cut(s) 845
BstMAI GTCTC 3 cut(s) 106, 1073, 2397
BstNI CCWGG 1 cut(s) 2111
BstNSI RCATGY 2 cut(s) 703, 2783
BstPAI GACNNNNGTC 1 cut(s) 98
BstPI GGTNACC 1 cut(s) 2685
BstSCI CCNGG 3 cut(s) 703, 1327, 2109
BstSFI CTRYAG 4 cut(s) 273, 1572, 2235, 2695
BstSLI GKGCMC 1 cut(s) 699
BstV2I GAAGAC 5 cut(s) 43, 98, 679, 1027, 1801
BstX2I RGATCY 2 cut(s) 1072, 1362
BstYI RGATCY 2 cut(s) 1072, 1362
Bsu36I CCTNAGG 1 cut(s) 1350
BsuRI GGCC 6 cut(s) 179, 708, 1004, 1327, 1867, 1893
BtgI CCRYGG 2 cut(s) 999, 2469
BtgZI GCGATG 1 cut(s) 916
BtrI CACGTC 1 cut(s) 1296
BtsCI GGATG 6 cut(s) 509, 640, 756, 1361, 1414, 2119
BtsI GCAGTG 2 cut(s) 1632, 2027
BtsIMutI CAGTG 7 cut(s) 111, 845, 1407, 1632, 1661, 1887, 2027
Cac8I GCNNGC 4 cut(s) 309, 1788, 2530, 2781
CciI TCATGA 1 cut(s) 777
CfoI GCGC 1 cut(s) 845
Cfr13I GGNCC 7 cut(s) 283, 320, 707, 858, 1326, 1865, 2306
CseI GACGC 2 cut(s) 581, 2521
Csp6I GTAC 6 cut(s) 18, 429, 541, 1436, 2363, 2742
CviQI GTAC 6 cut(s) 18, 429, 541, 1436, 2363, 2742
DrdI GACNNNNNNGTC 1 cut(s) 1294
DriI GACNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 1294
EaeI YGGCCR 2 cut(s) 177, 1002
Eam1105I GACNNNNNGTC 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 2640
Eco130I CCWWGG 5 cut(s) 732, 999, 1122, 1609, 2469
Eco24I GRGCYC 1 cut(s) 2642
Eco47I GGWCC 4 cut(s) 283, 320, 858, 2306
Eco53kI GAGCTC 1 cut(s) 2640
Eco57I CTGAAG 1 cut(s) 804
Eco81I CCTNAGG 1 cut(s) 1350
Eco91I GGTNACC 1 cut(s) 2685
EcoICRI GAGCTC 1 cut(s) 2640
EcoNI CCTNNNNNAGG 1 cut(s) 1126
EcoO109I RGGNCCY 1 cut(s) 858
EcoO65I GGTNACC 1 cut(s) 2685
EcoRII CCWGG 1 cut(s) 2109
EcoT14I CCWWGG 5 cut(s) 732, 999, 1122, 1609, 2469
EcoT22I ATGCAT 2 cut(s) 994, 1750
EcoT38I GRGCYC 1 cut(s) 2642
ErhI CCWWGG 5 cut(s) 732, 999, 1122, 1609, 2469
FaqI GGGAC 7 cut(s) 99, 254, 333, 584, 1033, 1790, 2292
FauI CCCGC 1 cut(s) 1923
FauNDI CATATG 3 cut(s) 976, 1322, 1522
FbaI TGATCA 3 cut(s) 637, 1275, 2671
FokI GGATG 6 cut(s) 516, 647, 763, 1368, 1421, 2126
FriOI GRGCYC 1 cut(s) 2642
FspBI CTAG 7 cut(s) 681, 713, 746, 953, 1076, 1956, 2805
GlaI GCGC 1 cut(s) 844
GsuI CTGGAG 2 cut(s) 229, 1242
HaeIII GGCC 6 cut(s) 179, 708, 1004, 1327, 1867, 1893
HapII CCGG 3 cut(s) 705, 1329, 1735
HgaI GACGC 2 cut(s) 581, 2521
HhaI GCGC 1 cut(s) 845
Hin1I GRCGYC 3 cut(s) 573, 1491, 2532
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HindIII AAGCTT 4 cut(s) 495, 1027, 2045, 2264
HpaII CCGG 3 cut(s) 705, 1329, 1735
HphI GGTGA 8 cut(s) 91, 263, 865, 1276, 1978, 2386, 2679, 2681
Hpy166II GTNNAC 2 cut(s) 697, 1672
Hpy188III TCNNGA 8 cut(s) 125, 246, 778, 1076, 1759, 1835, 2437, 2858
Hpy8I GTNNAC 2 cut(s) 697, 1672
Hpy99I CGWCG 2 cut(s) 482, 575
HpyAV CCTTC 7 cut(s) 417, 431, 755, 1399, 1505, 1813, 2407
HpyCH4III ACNGT 6 cut(s) 1179, 1665, 2038, 2489, 2627, 2741
HpyCH4IV ACGT 4 cut(s) 1295, 1438, 1491, 2146
HpySE526I ACGT 4 cut(s) 1295, 1438, 1491, 2146
Hsp92I GRCGYC 3 cut(s) 573, 1491, 2532
HspAI GCGC 1 cut(s) 843
Ksp22I TGATCA 3 cut(s) 637, 1275, 2671
LmnI GCTCC 6 cut(s) 652, 1223, 1346, 2291, 2645, 2848
LweI GCATC 4 cut(s) 177, 253, 970, 1612
MaeI CTAG 7 cut(s) 681, 713, 746, 953, 1076, 1956, 2805
MaeII ACGT 4 cut(s) 1295, 1438, 1491, 2146
MaeIII GTNAC 8 cut(s) 886, 1179, 1528, 1659, 2006, 2192, 2533, 2685
MbiI CCGCTC 1 cut(s) 2683
MfeI CAATTG 1 cut(s) 1233
MflI RGATCY 2 cut(s) 1072, 1362
MhlI GDGCHC 3 cut(s) 699, 1992, 2642
MlyI GAGTC 8 cut(s) 146, 236, 693, 1947, 1993, 2026, 2224, 2399
MmeI TCCRAC 2 cut(s) 956, 1372
Mph1103I ATGCAT 2 cut(s) 994, 1750
MroXI GAANNNNTTC 1 cut(s) 1857
MslI CAYNNNNRTG 2 cut(s) 2188, 2575
MspA1I CMGCKG 2 cut(s) 269, 1930
MspI CCGG 3 cut(s) 705, 1329, 1735
MspR9I CCNGG 3 cut(s) 705, 1329, 2111
MunI CAATTG 1 cut(s) 1233
MvaI CCWGG 1 cut(s) 2111
NciI CCSGG 2 cut(s) 705, 1329
NcoI CCATGG 2 cut(s) 999, 2469
NdeI CATATG 3 cut(s) 976, 1322, 1522
NlaIV GGNNCC 3 cut(s) 321, 1342, 2308
NmeAIII GCCGAG 1 cut(s) 700
NmuCI GTSAC 4 cut(s) 886, 1659, 2533, 2685
NsiI ATGCAT 2 cut(s) 994, 1750
NspI RCATGY 2 cut(s) 703, 2783
PaeI GCATGC 1 cut(s) 2783
PagI TCATGA 1 cut(s) 777
PcsI WCGNNNNNNNCGW 1 cut(s) 1292
PdmI GAANNNNTTC 1 cut(s) 1857
PfeI GAWTC 8 cut(s) 121, 173, 458, 1479, 1637, 1694, 2383, 2480
PflMI CCANNNNNTGG 1 cut(s) 1373
PleI GAGTC 8 cut(s) 145, 236, 692, 1946, 1993, 2025, 2223, 2399
PpsI GAGTC 8 cut(s) 145, 236, 692, 1946, 1993, 2025, 2223, 2399
Ppu21I YACGTR 1 cut(s) 1439
PpuMI RGGWCCY 1 cut(s) 858
PshAI GACNNNNGTC 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 1281
Psp124BI GAGCTC 1 cut(s) 2642
Psp1406I AACGTT 1 cut(s) 2146
Psp5II RGGWCCY 1 cut(s) 858
Psp6I CCWGG 1 cut(s) 2109
PspEI GGTNACC 1 cut(s) 2685
PspGI CCWGG 1 cut(s) 2109
PspN4I GGNNCC 3 cut(s) 321, 1342, 2308
PspPI GGNCC 7 cut(s) 283, 320, 707, 858, 1326, 1865, 2306
PspPPI RGGWCCY 1 cut(s) 858
PstI CTGCAG 4 cut(s) 277, 1576, 2239, 2699
PsuI RGATCY 2 cut(s) 1072, 1362
RsaI GTAC 6 cut(s) 19, 430, 542, 1437, 2364, 2743
RsaNI GTAC 6 cut(s) 18, 429, 541, 1436, 2363, 2742
RseI CAYNNNNRTG 2 cut(s) 2188, 2575
SacI GAGCTC 1 cut(s) 2642
Sau96I GGNCC 7 cut(s) 283, 320, 707, 858, 1326, 1865, 2306
SchI GAGTC 8 cut(s) 146, 236, 693, 1947, 1993, 2026, 2224, 2399
ScrFI CCNGG 3 cut(s) 705, 1329, 2111
SduI GDGCHC 3 cut(s) 699, 1992, 2642
SfaNI GCATC 4 cut(s) 177, 253, 970, 1612
SfcI CTRYAG 4 cut(s) 273, 1572, 2235, 2695
SinI GGWCC 4 cut(s) 283, 320, 858, 2306
SmiMI CAYNNNNRTG 2 cut(s) 2188, 2575
SmlI CTYRAG 3 cut(s) 134, 465, 2048
SmoI CTYRAG 3 cut(s) 134, 465, 2048
SphI GCATGC 1 cut(s) 2783
SsiI CCGC 3 cut(s) 267, 1930, 2683
SspI AATATT 4 cut(s) 799, 1203, 2164, 2775
SspMI CTAG 7 cut(s) 681, 713, 746, 953, 1076, 1956, 2805
SstI GAGCTC 1 cut(s) 2642
StyD4I CCNGG 3 cut(s) 703, 1327, 2109
StyI CCWWGG 5 cut(s) 732, 999, 1122, 1609, 2469
TaaI ACNGT 6 cut(s) 1179, 1665, 2038, 2489, 2627, 2741
TaiI ACGT 4 cut(s) 1298, 1441, 1494, 2149
TaqI TCGA 8 cut(s) 171, 261, 570, 607, 1286, 2381, 2408, 2720
TaqII GACCGA 1 cut(s) 337
TatI WGTACW 2 cut(s) 17, 428
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 8 cut(s) 121, 173, 458, 1479, 1637, 1694, 2383, 2480
TscAI CASTG 7 cut(s) 111, 852, 1407, 1632, 1668, 1894, 2034
TseFI GTSAC 4 cut(s) 886, 1659, 2533, 2685
Tsp45I GTSAC 4 cut(s) 886, 1659, 2533, 2685
TspGWI ACGGA 2 cut(s) 564, 647
TspRI CASTG 7 cut(s) 111, 852, 1407, 1632, 1668, 1894, 2034
Van91I CCANNNNNTGG 1 cut(s) 1373
VneI GTGCAC 1 cut(s) 695
VpaK11BI GGWCC 4 cut(s) 283, 320, 858, 2306
VspI ATTAAT 1 cut(s) 1281
XagI CCTNNNNNAGG 1 cut(s) 1126
XapI RAATTY 6 cut(s) 1136, 1156, 1306, 2662, 2722, 2795
XbaI TCTAGA 1 cut(s) 1075
XceI RCATGY 2 cut(s) 703, 2783
XmnI GAANNNNTTC 1 cut(s) 1857
XspI CTAG 7 cut(s) 681, 713, 746, 953, 1076, 1956, 2805
ZraI GACGTC 1 cut(s) 1492
Zsp2I ATGCAT 2 cut(s) 994, 1750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.