Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000051

Physical Location & Seq

Genomic Coordinates Reverse (-)

3966898 .. 3969838

Gene Structure 2941 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1059500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGCAAGCCATCATAGGGTTAGAATCATCAATGCAAGCCAGCTTTGTAATTGAGCTTGGAGATAAAGCTCAGGTGCCCATAATCTCATTTTCCGCAACAAGCCCTTCTTTTCAAGGTTCATACTTTTTCCGAATTGCACAAAATGACTCATCTCAAGTTAAAGCCATTAGTAATATCATCCAAGCCTTTGGTTGGAGTGAAGCAGTGCCCATCAGCGTTGACAATGAGTTCGGAAAAGGAGTAATACCTTACCTGACTACTGCTTTGCAATCAGTTGGTGCTCGTGTGCCCTACTGGAATTCCATTCCTTCAATCTCCACTGATGAGGAAATTGTTGCAGAGCTTCGCAAGTTGATGTCAATGAGAACCAGGGTCTTCATAGTGCATATGTCCCCATATCTTGGCTCTCGGTTTTTCTCTAAAGCAAAAGACTTTGGCATGATGGATGAAGGCTATGTTTGGATACTGACAAATGGGATGACTAACGGTTTTATTTCCTCGAGTTCTTATGTCAACATAGATGACATGCAAGGGGTAGCTAGATGGCAAACAAAATTCCAACAAGACAATCCAACTATCCCTAGTGTTAAACTGGATGTTTTTGGATTGTGGGCATACGATGCCGCTTGGGCACTAGCCATGGCTGCTGAGAAGGTTGGAATAACAAACTTCCGCGTCCAGAAGATTAACAATTCTGGCAATTCCATTGGTTCAAAGAGATTAGAGGTCTCTCAAAGTGGTACTGAACTTGTCCGAGAACTATCAGGTACAAGATTCCAAGGTCTTTTGGGAGATTTCAGCCTTATTAATAAGCAACTACAATCATCAACTTTTCAGGTAGTTAATGTGAATGATAATGGGGAAAGAGGGATTGGATATTGGACACCCAAAACTGGACTTGTAAGAAACATTAAATCGGACAAAAATACAAGCAGACACTCTACTTCTAATGCCAGTCTTGGACCTTTTATATGGCCTGAAGACACCACCTCGGTTCCAAGGGGTTGGCAGATTAGTGCGAATGGGACCTTAAGAATTGGAGTCCCGGTGAAGGGAGCATATGACGAACTTGTAAGTGTGAAACATGATCCAAGCACTAATACAACCAATATCACTGGATACTGCATTGACGTCTTTACTGCGGTAATAGAAGTATTGCCGTACTTTGTTCCTTATGAGTTTCATCCCTTTACAAGGCCTGATGGTACGAGAGCTAGTTACAATTATTTGCTCAATCAAGTAATTCTTGGGAAATATGATGCTGCAGTTGGAGATATTACCATTAGAGGAAATAAATCTTTGTTTGTTGACTTTACATTGCCATACACAGAAGCATGGGTATCGATGGTCGTGCCTTTCAAAGGAAATACAGGTGCCAAAAACACCTGGGTGTTCCTGAAGCCTTTGACTTGGGACCTTTGGGCAACCAGTGCCTGTTTTTTCATATTCATTGGTTATGTGCAAGTATTGAGCAACTTGGCTAGATTTGTAGTGATTATATGGTACTTTGTTGTTCTTGTACTAACACAAAATTATACAGCCAGTTTAACATCAATGTTAACAATCCAACAGCTTCAACCAGTCGTTACTGATGTAAACCTTCTCATAAAGAATGGGGACAAGGTTGGTTACGACGATAAACTCAAGCCCTATCATTCTAGAGAAGAATTGAAGAAACTTCTTCGCAACAGGAACAAAAATGGAGGTATTTCTGCGGCTTTTGATGAAACTCCCTACATGAAACCTTTTATTGCAACATATTGCTCAGAATGTACCCTTGTTACACCAACATTCAACTCTGATGGGTTTGCATTTGTCTTTCAAAAAGGTTTGCTTCTCACGCGTGATTTTTCAACAGCAATCACTAAAGTGAGCGAGGGAGGTAAAATGGAGGCCATCGAAGATAAGTGGTTCAAGAAAAATGAAAGTTGTTCAAACTCCAACACCATGGAAAGTTCCAACACTCTTAGTCTTGATAGCTTTAGGGGCCTCTTCATCGTTGCTGGTGTTGCTTCATCACTAGCTATCCTCGTATTTACAGCTATGTTCCTGTATGAGCATGGCCACAACTTGACACGGGCAGATTCAGAAGCCTCATTTTGGACAAGAATTCATGAAATATTAGGAGTATATTACGGAAATGACCTCACTTCAGATACTTCTAAGAATAGCACGCTGCAAAATAGTGATCATGGTATAGCTGTGGCTGAATCTTCAGCAAACAGCAAATGTCCCCCTAAGTCATCATCAAGCTCTCCCGACGCCACTGGACCGCACATTGTTCATCAAGAGTTGGTGGAAACTCCTAATTCTTCCGTGCATGGTGGTGATCGGAGTCCCAATGGTCAAGCAACCCAAGACAGTGGTACTCATGAATATGGTATTGAGTTAACTTCCTTCCCAAGTGATGAGAAACAGACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

808

Amino Acids

89.17

Weight (kDa)

5.97

Isoelectric Point (pI)

34.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANF_receptor	PF01094	2 - 285	4.3e-59	Receptor family ligand binding region
Peripla_BP_6	PF13458	2 - 284	3.9e-10	Periplasmic binding protein
Lig_chan-Glu_bd	PF10613	364 - 451	6.8e-07	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	367 - 639	2.6e-07	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	473 - 671	9.5e-20	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Image

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Publication-ready

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Orthologous Genes (Group: OG0000051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24710 AT2G24710 AT2G24710 AT2G24720 AT2G29100 AT2G29100 AT2G29110 AT2G29110 AT2G29120 AT5G27100
fragaria_vesca	FvH4_2g11260 FvH4_2g11280 FvH4_2g11310 FvH4_2g11310 FvH4_2g11331 FvH4_2g11331 FvH4_2g11340 FvH4_2g11340 FvH4_2g11340 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31990 FvH4_2g37140 FvH4_2g37150 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37161 FvH4_2g37161 FvH4_2g37163 FvH4_2g37163 FvH4_2g37170 FvH4_2g37181 FvH4_2g37191 FvH4_3g22781 FvH4_3g22781 FvH4_3g22781 FvH4_3g22790 FvH4_3g22790 FvH4_3g22810 FvH4_5g13460 FvH4_5g34751 FvH4_7g09130 FvH4_7g27991 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010
malus_domestica	MD08G1047600.v1.1 MD08G1047700.v1.1 MD08G1048100.v1.1 MD08G1048200.v1.1 MD08G1048300.v1.1 MD08G1048500.v1.1 MD08G1048600.v1.1 MD08G1048700.v1.1 MD08G1048900.v1.1 MD08G1049000.v1.1 MD14G1064700.v1.1 MD15G1045900.v1.1 MD15G1046000.v1.1 MD15G1046200.v1.1 MD15G1046400.v1.1 MD15G1059200.v1.1 MD15G1059500.v1.1 MD15G1059600.v1.1 MD15G1059700.v1.1
prunus_persica	Prupe.1G399500_v2.0.a1 Prupe.1G399600_v2.0.a1 Prupe.1G399700_v2.0.a1 Prupe.1G399800_v2.0.a1 Prupe.1G399900_v2.0.a1 Prupe.1G400000_v2.0.a1 Prupe.1G400300_v2.0.a1 Prupe.1G400400_v2.0.a1 Prupe.1G400500_v2.0.a1 Prupe.1G400600_v2.0.a1 Prupe.1G400700_v2.0.a1 Prupe.1G412700_v2.0.a1 Prupe.1G412800_v2.0.a1
pyrus_communis	pycom02g01350 pycom08g04020 pycom08g04040 pycom08g04080 pycom08g04090 pycom08g04100 pycom14g05240 pycom15g04300 pycom15g04310 pycom15g04330 pycom15g04340 pycom15g04380 pycom15g05560 pycom15g05570 pycom15g05580 pycom15g05600
rosa_chinensis	RchiOBHm_Chr1g0345071 RchiOBHm_Chr1g0345081 RchiOBHm_Chr1g0345171 RchiOBHm_Chr3g0480811 RchiOBHm_Chr3g0492581 RchiOBHm_Chr5g0020871 RchiOBHm_Chr5g0039801 RchiOBHm_Chr5g0039811 RchiOBHm_Chr5g0039861 RchiOBHm_Chr5g0039911 RchiOBHm_Chr5g0039941 RchiOBHm_Chr5g0039951 RchiOBHm_Chr5g0040011 RchiOBHm_Chr6g0269601 RchiOBHm_Chr6g0269621 RchiOBHm_Chr6g0269631 RchiOBHm_Chr6g0269641 RchiOBHm_Chr6g0269671 RchiOBHm_Chr6g0269681 RchiOBHm_Chr6g0269731 RchiOBHm_Chr6g0269751 RchiOBHm_Chr6g0269761 RchiOBHm_Chr6g0269771 RchiOBHm_Chr6g0269781 RchiOBHm_Chr6g0269861 RchiOBHm_Chr6g0269871 RchiOBHm_Chr6g0269881 RchiOBHm_Chr6g0269911 RchiOBHm_Chr6g0269921 RchiOBHm_Chr6g0269941 RchiOBHm_Chr6g0269951 RchiOBHm_Chr6g0304511 RchiOBHm_Chr6g0304621 RchiOBHm_Chr6g0304641 RchiOBHm_Chr6g0304651 RchiOBHm_Chr6g0304661 RchiOBHm_Chr6g0310781 RchiOBHm_Chr6g0310801 RchiOBHm_Chr6g0310811 RchiOBHm_Chr6g0310821 RchiOBHm_Chr6g0310831 RchiOBHm_Chr7g0183621 RchiOBHm_Chr7g0183651
rosa_laevigata	RLG00000005035 RLG00000005036 RLG00000010458 RLG00000010459 RLG00000010460 RLG00000010961 RLG00000010962 RLG00000010963 RLG00000010964 RLG00000010966 RLG00000010970 RLG00000013824 RLG00000013825 RLG00000013826 RLG00000013828 RLG00000013829 RLG00000013831 RLG00000013833 RLG00000013835 RLG00000013837 RLG00000013840 RLG00000013842 RLG00000013844 RLG00000022758 RLG00000023449 RLG00000026705 RLG00000028849 RLG00000028853 RLG00000028854 RLG00000032569 RLG00000033944 RLG00000033945 RLG00000033947 RLG00000033949 RLG00000033951 RLG00000034239
rosa_multiflora	Rmu_co8287891.1_g000001 Rmu_co8428745.1_g000001 Rmu_co8521767.1_g000002 Rmu_sc0000103.1_g000022 Rmu_sc0000103.1_g000031 Rmu_sc0000103.1_g000041 Rmu_sc0000103.1_g000053 Rmu_sc0000247.1_g000028 Rmu_sc0000247.1_g000051 Rmu_sc0000893.1_g000003 Rmu_sc0000893.1_g000017 Rmu_sc0001143.1_g000008 Rmu_sc0001143.1_g000019 Rmu_sc0001143.1_g000023 Rmu_sc0001975.1_g000003 Rmu_sc0001975.1_g000004 Rmu_sc0001975.1_g000005 Rmu_sc0001975.1_g000012 Rmu_sc0002151.1_g000006 Rmu_sc0003610.1_g000021 Rmu_sc0003682.1_g000001 Rmu_sc0003863.1_g000004 Rmu_sc0003863.1_g000007 Rmu_sc0003863.1_g000013 Rmu_sc0004196.1_g000005 Rmu_sc0004515.1_g000013 Rmu_sc0004515.1_g000024 Rmu_sc0004688.1_g000001 Rmu_sc0004688.1_g000003 Rmu_sc0004688.1_g000004 Rmu_sc0004748.1_g000006 Rmu_sc0004838.1_g000016 Rmu_sc0004838.1_g000017 Rmu_sc0005532.1_g000006 Rmu_sc0005532.1_g000011 Rmu_sc0005950.1_g000004 Rmu_sc0006576.1_g000005 Rmu_sc0006576.1_g000021 Rmu_sc0008520.1_g000002 Rmu_sc0008520.1_g000004 Rmu_sc0009130.1_g000002 Rmu_sc0018719.1_g000001 Rmu_sc0027713.1_g000005 Rmu_sc0040804.1_g000001 Rmu_ssc0000218.1_g000059 Rmu_ssc0000218.1_g000062 Rmu_ssc0000218.1_g000068 Rmu_ssc0000395.1_g000008 Rmu_ssc0000395.1_g000009 Rmu_ssc0000395.1_g000010 Rmu_ssc0000395.1_g000011 Rmu_ssc0000395.1_g000013 Rmu_ssc0000395.1_g000014
rosa_roxburghii	Rroxscaffold_1G00041270 Rroxscaffold_1G00041290 Rroxscaffold_1G00041300 Rroxscaffold_1G00041360 Rroxscaffold_1G00041380 Rroxscaffold_1G00057500 Rroxscaffold_3G00270350 Rroxscaffold_4G00283160 Rroxscaffold_4G00309210 Rroxscaffold_4G00309240 Rroxscaffold_4G00309260 Rroxscaffold_4G00309290 Rroxscaffold_4G00309360 Rroxscaffold_6G00392210 Rroxscaffold_6G00401030 Rroxscaffold_7G00158260 Rroxscaffold_7G00163620 Rroxscaffold_7G00163630 Rroxscaffold_7G00163650 Rroxscaffold_7G00163680 Rroxscaffold_7G00198270 Rroxscaffold_7G00198280 Rroxscaffold_7G00198330 Rroxscaffold_7G00198340 Rroxscaffold_7G00198360 Rroxscaffold_7G00198390 Rroxscaffold_7G00198410 Rroxscaffold_7G00198450 Rroxscaffold_7G00198460 Rroxscaffold_7G00198490 Rroxscaffold_7G00198500 Rroxscaffold_7G00198510 Rroxscaffold_7G00198530 Rroxscaffold_7G00198550 Rroxscaffold_7G00198600
rosa_rugosa	Rorug01G0175900 Rorug01G0176200 Rorug03G0186700 Rorug03G0254500 Rorug05G0182700 Rorug05G0183000 Rorug05G0183000 Rorug06G0049000 Rorug06G0049500 Rorug06G0049600.1 Rorug06G0049700 Rorug06G0050000 Rorug06G0050000 Rorug06G0050100 Rorug06G0050300.1 Rorug06G0050400 Rorug06G0051100 Rorug06G0051200 Rorug06G0051300 Rorug06G0051500 Rorug06G0051600 Rorug06G0051700 Rorug06G0332600 Rorug06G0332700 Rorug06G0332700 Rorug06G0383100 Rorug06G0383200 Rorug06G0383300 Rorug06G0383400 Rorug06G0459100 Rorug06G0459200
rosa_samantha	Rh1BG160400 Rh1BG160500 Rh1BG160700 Rh3DG263900 Rh3DG338400 Rh5CG164400 Rh5CG164500 Rh5CG305500 Rh5CG305900 Rh5CG306000 Rh5DG280800 Rh5DG281300 Rh6AG445300 Rh6AG497000 Rh6BG174700 Rh6BG457200 Rh6CG168900 Rh6CG169800 Rh6CG512700 Rh6CG512900 Rh6DG034100 Rh6DG160500 Rh6DG160800 Rh6DG160900 Rh6DG161100 Rh6DG161400 Rh6DG161800 Rh6DG162000 Rh6DG162100 Rh6DG162200 Rh6DG444800 Rh6DG445000 Rh6DG445100 Rh6DG445200 Rh6DG445300 Rh6DG499100 Rh6DG499200 Rh6DG499300 Rh6DG499400 Rh6DG499500 Rh6DG499700 Rh7BG059800 Rh7BG060000 Rh7BG258500 Rh7BG258700
rosa_wichuraiana	Rw0G006180 Rw0G007950 Rw0G018490 Rw0G022090 Rw1G015970 Rw1G015990 Rw3G021330 Rw5G025120 Rw5G025140 Rw5G025150 Rw5G025160 Rw5G025180 Rw5G025830 Rw5G025850 Rw6G014550 Rw6G014570 Rw6G014580 Rw6G014600 Rw6G014620 Rw6G014650 Rw6G014670 Rw6G038650 Rw6G038670 Rw6G038680 Rw6G038700 Rw6G038710 Rw6G038720 Rw6G043280 Rw6G043290 Rw7G004960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1134
AccB1I	GGYRCC	2 cut(s)	73, 1373
AccB7I	CCANNNNNTGG	1 cut(s)	401
AccII	CGCG	2 cut(s)	675, 1843
AciI	CCGC	6 cut(s)	93, 624, 673, 1142, 1715, 2273
AclWI	GGATC	1 cut(s)	1082
AcoI	YGGCCR	1 cut(s)	2062
AcsI	RAATTY	3 cut(s)	298, 554, 2109
AcuI	CTGAAG	4 cut(s)	999, 1418, 2136, 2199
AcyI	GRCGYC	2 cut(s)	1131, 2262
AfaI	GTAC	8 cut(s)	742, 769, 1163, 1207, 1505, 1521, 1774, 2368
AfiI	CCNNNNNNNGG	9 cut(s)	15, 192, 401, 893, 1051, 1052, 1194, 1361, 2100
AflII	CTTAAG	1 cut(s)	1030
AflIII	ACRYGT	1 cut(s)	1841
AjnI	CCWGG	2 cut(s)	368, 1385
AjuI	GAANNNNNNNTTGG	2 cut(s)	855, 887
AleI	CACNNNNGTG	2 cut(s)	1388, 1868
Alw21I	GWGCWC	1 cut(s)	283
Alw26I	GTCTC	1 cut(s)	733
AlwI	GGATC	1 cut(s)	1082
AlwNI	CAGNNNCTG	1 cut(s)	1434
Ama87I	CYCGRG	1 cut(s)	499
AoxI	GGCC	5 cut(s)	974, 1196, 1893, 1987, 2062
ApeKI	GCWGC	3 cut(s)	644, 1262, 2176
ApoI	RAATTY	3 cut(s)	298, 554, 2109
ArsI	GACNNNNNNTTYG	4 cut(s)	212, 244, 247, 279
AseI	ATTAAT	1 cut(s)	807
AspS9I	GGNCC	5 cut(s)	962, 1026, 1414, 1987, 2270
AsuC2I	CCSGG	1 cut(s)	1046
AsuHPI	GGTGA	2 cut(s)	1060, 2339
AvaI	CYCGRG	1 cut(s)	499
AvaII	GGWCC	4 cut(s)	962, 1026, 1414, 2270
BaeGI	GKGCMC	4 cut(s)	78, 210, 291, 634
BalI	TGGCCA	1 cut(s)	2064
BanI	GGYRCC	2 cut(s)	73, 1373
BauI	CACGAG	1 cut(s)	282
BbsI	GAAGAC	2 cut(s)	367, 987
Bbv12I	GWGCWC	1 cut(s)	283
BbvI	GCAGC	3 cut(s)	631, 1249, 2163
BccI	CCATC	8 cut(s)	17, 218, 436, 537, 1196, 1339, 1796, 1904
BceAI	ACGGC	1 cut(s)	1144
BcgI	CGANNNNNNTGC	4 cut(s)	1323, 1333, 1357, 1367
BciT130I	CCWGG	2 cut(s)	370, 1387
BciVI	GTATCC	2 cut(s)	456, 1112
BclI	TGATCA	1 cut(s)	2188
BcnI	CCSGG	1 cut(s)	1046
BcoDI	GTCTC	1 cut(s)	733
BfaI	CTAG	7 cut(s)	540, 582, 635, 1215, 1482, 1659, 2021
BfmI	CTRYAG	1 cut(s)	1263
BfrI	CTTAAG	1 cut(s)	1030
BfuI	GTATCC	2 cut(s)	456, 1112
BglI	GCCNNNNNGGC	1 cut(s)	629
BisI	GCNGC	5 cut(s)	624, 645, 1263, 1716, 2177
BlsI	GCNGC	5 cut(s)	625, 646, 1264, 1717, 2178
Bme1390I	CCNGG	3 cut(s)	370, 1046, 1387
Bme18I	GGWCC	4 cut(s)	962, 1026, 1414, 2270
BmeT110I	CYCGRG	1 cut(s)	499
BmgT120I	GGNCC	5 cut(s)	962, 1026, 1414, 1987, 2270
BmiI	GGNNCC	6 cut(s)	75, 996, 1027, 1375, 1415, 1988
BmrFI	CCNGG	3 cut(s)	370, 1046, 1387
BmsI	GCATC	2 cut(s)	610, 1249
BpiI	GAAGAC	2 cut(s)	367, 987
Bpu10I	CCTNAGC	1 cut(s)	69
BpuEI	CTTGAG	2 cut(s)	138, 1628
BpuMI	CCSGG	1 cut(s)	1046
Bsa29I	ATCGAT	1 cut(s)	1343
BsaHI	GRCGYC	2 cut(s)	1131, 2262
BsaI	GGTCTC	1 cut(s)	733
BsaJI	CCNNGG	7 cut(s)	369, 639, 778, 990, 998, 1386, 1947
BsaXI	ACNNNNNCTCC	2 cut(s)	1032, 1062
Bsc4I	CCNNNNNNNGG	9 cut(s)	15, 192, 401, 893, 1051, 1052, 1194, 1361, 2100
Bse1I	ACTGG	9 cut(s)	299, 597, 898, 954, 1120, 1428, 1542, 1580, 2272
Bse3DI	GCAATG	1 cut(s)	1316
BseBI	CCWGG	2 cut(s)	370, 1387
BseCI	ATCGAT	1 cut(s)	1343
BseDI	CCNNGG	7 cut(s)	369, 639, 778, 990, 998, 1386, 1947
BseGI	GGATG	5 cut(s)	177, 451, 483, 601, 1183
BseLI	CCNNNNNNNGG	9 cut(s)	15, 192, 401, 893, 1051, 1052, 1194, 1361, 2100
BseMI	GCAATG	1 cut(s)	1316
BseMII	CTCAG	3 cut(s)	83, 639, 1779
BseNI	ACTGG	9 cut(s)	299, 597, 898, 954, 1120, 1428, 1542, 1580, 2272
BseSI	GKGCMC	4 cut(s)	78, 210, 291, 634
BseXI	GCAGC	3 cut(s)	631, 1249, 2163
Bsh1236I	CGCG	2 cut(s)	675, 1843
BshFI	GGCC	5 cut(s)	976, 1198, 1895, 1989, 2064
BshNI	GGYRCC	2 cut(s)	73, 1373
BshVI	ATCGAT	1 cut(s)	1343
BsiHKAI	GWGCWC	1 cut(s)	283
BsiHKCI	CYCGRG	1 cut(s)	499
BsiSI	CCGG	1 cut(s)	1046
BslFI	GGGAC	7 cut(s)	376, 1028, 1039, 1427, 1631, 2217, 2322
BslI	CCNNNNNNNGG	9 cut(s)	15, 192, 401, 893, 1051, 1052, 1194, 1361, 2100
BsmAI	GTCTC	1 cut(s)	733
BsmFI	GGGAC	7 cut(s)	376, 1028, 1039, 1427, 1631, 2217, 2322
BsnI	GGCC	5 cut(s)	976, 1198, 1895, 1989, 2064
Bso31I	GGTCTC	1 cut(s)	733
BsoBI	CYCGRG	1 cut(s)	499
Bsp1286I	GDGCHC	5 cut(s)	78, 210, 283, 291, 634
Bsp143I	GATC	3 cut(s)	1087, 2188, 2329
Bsp19I	CCATGG	2 cut(s)	639, 1947
BspACI	CCGC	6 cut(s)	93, 624, 673, 1142, 1715, 2273
BspANI	GGCC	5 cut(s)	976, 1198, 1895, 1989, 2064
BspCNI	CTCAG	3 cut(s)	82, 640, 1778
BspDI	ATCGAT	1 cut(s)	1343
BspFNI	CGCG	2 cut(s)	675, 1843
BspHI	TCATGA	2 cut(s)	2113, 2371
BspLI	GGNNCC	6 cut(s)	75, 996, 1027, 1375, 1415, 1988
BspMAI	CTGCAG	1 cut(s)	1267
BspPI	GGATC	1 cut(s)	1082
BspT107I	GGYRCC	2 cut(s)	73, 1373
BspTI	CTTAAG	1 cut(s)	1030
BspTNI	GGTCTC	1 cut(s)	733
BsrDI	GCAATG	1 cut(s)	1316
BsrI	ACTGG	9 cut(s)	299, 597, 898, 954, 1120, 1428, 1542, 1580, 2272
BssECI	CCNNGG	7 cut(s)	369, 639, 778, 990, 998, 1386, 1947
BssMI	GATC	3 cut(s)	1087, 2188, 2329
BssNI	GRCGYC	2 cut(s)	1131, 2262
BssSI	CACGAG	1 cut(s)	282
BssT1I	CCWWGG	4 cut(s)	639, 778, 998, 1947
Bst2BI	CACGAG	1 cut(s)	282
Bst2UI	CCWGG	2 cut(s)	370, 1387
Bst4CI	ACNGT	2 cut(s)	488, 2363
Bst6I	CTCTTC	1 cut(s)	1997
BstACI	GRCGYC	2 cut(s)	1131, 2262
BstAFI	CTTAAG	1 cut(s)	1030
BstAPI	GCANNNNNTGC	2 cut(s)	620, 1430
BstC8I	GCNNGC	4 cut(s)	6, 36, 40, 2174
BstDEI	CTNAG	6 cut(s)	69, 648, 1765, 1967, 2163, 2238
BstDSI	CCRYGG	2 cut(s)	639, 1947
BstENI	CCTNNNNNAGG	2 cut(s)	1192, 1359
BstF5I	GGATG	5 cut(s)	177, 451, 483, 601, 1183
BstFNI	CGCG	2 cut(s)	675, 1843
BstKTI	GATC	3 cut(s)	1090, 2191, 2332
BstMAI	GTCTC	1 cut(s)	733
BstMBI	GATC	3 cut(s)	1087, 2188, 2329
BstMWI	GCNNNNNNNGC	7 cut(s)	620, 629, 644, 1430, 1840, 1986, 2009
BstNI	CCWGG	2 cut(s)	370, 1387
BstNSI	RCATGY	1 cut(s)	529
BstSCI	CCNGG	3 cut(s)	368, 1044, 1385
BstSFI	CTRYAG	1 cut(s)	1263
BstSLI	GKGCMC	4 cut(s)	78, 210, 291, 634
BstUI	CGCG	2 cut(s)	675, 1843
BstV1I	GCAGC	3 cut(s)	631, 1249, 2163
BstV2I	GAAGAC	2 cut(s)	367, 987
BstXI	CCANNNNNNTGG	4 cut(s)	188, 1005, 1948, 2363
Bsu15I	ATCGAT	1 cut(s)	1343
BsuI	GTATCC	2 cut(s)	456, 1112
BsuRI	GGCC	5 cut(s)	976, 1198, 1895, 1989, 2064
BsuTUI	ATCGAT	1 cut(s)	1343
BtgI	CCRYGG	2 cut(s)	639, 1947
BtsCI	GGATG	5 cut(s)	177, 451, 483, 601, 1183
BtsI	GCAGTG	1 cut(s)	210
BtsIMutI	CAGTG	6 cut(s)	210, 318, 1113, 1435, 2265, 2368
Cac8I	GCNNGC	4 cut(s)	6, 36, 40, 2174
CaiI	CAGNNNCTG	1 cut(s)	1434
CciI	TCATGA	2 cut(s)	2113, 2371
Cfr13I	GGNCC	5 cut(s)	962, 1026, 1414, 1987, 2270
ClaI	ATCGAT	1 cut(s)	1343
CseI	GACGC	2 cut(s)	664, 2270
Csp6I	GTAC	8 cut(s)	741, 768, 1162, 1206, 1504, 1520, 1773, 2367
CviQI	GTAC	8 cut(s)	741, 768, 1162, 1206, 1504, 1520, 1773, 2367
DdeI	CTNAG	6 cut(s)	69, 648, 1765, 1967, 2163, 2238
DpnI	GATC	3 cut(s)	1089, 2190, 2331
DpnII	GATC	3 cut(s)	1087, 2188, 2329
EaeI	YGGCCR	1 cut(s)	2062
Eam1104I	CTCTTC	1 cut(s)	1997
EarI	CTCTTC	1 cut(s)	1997
Eco130I	CCWWGG	4 cut(s)	639, 778, 998, 1947
Eco147I	AGGCCT	1 cut(s)	1198
Eco31I	GGTCTC	1 cut(s)	733
Eco47I	GGWCC	4 cut(s)	962, 1026, 1414, 2270
Eco57I	CTGAAG	4 cut(s)	999, 1418, 2136, 2199
Eco88I	CYCGRG	1 cut(s)	499
EcoNI	CCTNNNNNAGG	2 cut(s)	1192, 1359
EcoO109I	RGGNCCY	3 cut(s)	1026, 1414, 1987
EcoRI	GAATTC	2 cut(s)	298, 2109
EcoRII	CCWGG	2 cut(s)	368, 1385
EcoT14I	CCWWGG	4 cut(s)	639, 778, 998, 1947
ErhI	CCWWGG	4 cut(s)	639, 778, 998, 1947
FalI	AAGNNNNNCTT	6 cut(s)	91, 123, 1230, 1262, 1818, 1850
FaqI	GGGAC	7 cut(s)	376, 1028, 1039, 1427, 1631, 2217, 2322
FauNDI	CATATG	2 cut(s)	387, 1060
FbaI	TGATCA	1 cut(s)	2188
Fnu4HI	GCNGC	5 cut(s)	624, 645, 1263, 1716, 2177
FokI	GGATG	5 cut(s)	164, 458, 490, 608, 1170
Fsp4HI	GCNGC	5 cut(s)	624, 645, 1263, 1716, 2177
FspBI	CTAG	7 cut(s)	540, 582, 635, 1215, 1482, 1659, 2021
GluI	GCNGC	5 cut(s)	624, 645, 1263, 1716, 2177
HaeIII	GGCC	5 cut(s)	976, 1198, 1895, 1989, 2064
HapII	CCGG	1 cut(s)	1046
HgaI	GACGC	2 cut(s)	664, 2270
Hin1I	GRCGYC	2 cut(s)	1131, 2262
HincII	GTYRAC	5 cut(s)	220, 514, 1309, 1560, 2391
HindII	GTYRAC	5 cut(s)	220, 514, 1309, 1560, 2391
HinfI	GANTC	7 cut(s)	23, 146, 774, 1041, 2084, 2210, 2335
HpaI	GTTAAC	2 cut(s)	1560, 2391
HpaII	CCGG	1 cut(s)	1046
HphI	GGTGA	2 cut(s)	1060, 2339
Hpy166II	GTNNAC	6 cut(s)	220, 514, 1309, 1560, 1597, 2391
Hpy188I	TCNGA	9 cut(s)	131, 233, 755, 919, 1768, 1801, 2089, 2155, 2334
Hpy188III	TCNNGA	9 cut(s)	679, 1396, 1659, 1915, 1973, 2114, 2258, 2288, 2372
Hpy8I	GTNNAC	6 cut(s)	220, 514, 1309, 1560, 1597, 2391
Hpy99I	CGWCG	2 cut(s)	1637, 2264
HpyAV	CCTTC	7 cut(s)	114, 318, 443, 646, 1045, 1610, 2407
HpyCH4III	ACNGT	2 cut(s)	488, 2363
HpyCH4IV	ACGT	1 cut(s)	1131
HpyF10VI	GCNNNNNNNGC	7 cut(s)	620, 629, 644, 1430, 1840, 1986, 2009
HpyF3I	CTNAG	6 cut(s)	69, 648, 1765, 1967, 2163, 2238
HpySE526I	ACGT	1 cut(s)	1131
Hsp92I	GRCGYC	2 cut(s)	1131, 2262
Ksp22I	TGATCA	1 cut(s)	2188
KspAI	GTTAAC	2 cut(s)	1560, 2391
Kzo9I	GATC	3 cut(s)	1087, 2188, 2329
LmnI	GCTCC	1 cut(s)	1055
Lsp1109I	GCAGC	3 cut(s)	631, 1249, 2163
LweI	GCATC	2 cut(s)	610, 1249
MaeI	CTAG	7 cut(s)	540, 582, 635, 1215, 1482, 1659, 2021
MaeII	ACGT	1 cut(s)	1131
MaeIII	GTNAC	4 cut(s)	1217, 1585, 1628, 1780
MalI	GATC	3 cut(s)	1089, 2190, 2331
MboI	GATC	3 cut(s)	1087, 2188, 2329
MhlI	GDGCHC	5 cut(s)	78, 210, 283, 291, 634
MlsI	TGGCCA	1 cut(s)	2064
MluI	ACGCGT	1 cut(s)	1841
MluNI	TGGCCA	1 cut(s)	2064
MlyI	GAGTC	3 cut(s)	140, 1050, 2344
MmeI	TCCRAC	8 cut(s)	173, 583, 596, 637, 1249, 1591, 1965, 1983
Mox20I	TGGCCA	1 cut(s)	2064
MscI	TGGCCA	1 cut(s)	2064
MslI	CAYNNNNRTG	4 cut(s)	1388, 1868, 2325, 2376
Msp20I	TGGCCA	1 cut(s)	2064
MspCI	CTTAAG	1 cut(s)	1030
MspI	CCGG	1 cut(s)	1046
MspR9I	CCNGG	3 cut(s)	370, 1046, 1387
MvaI	CCWGG	2 cut(s)	370, 1387
MvnI	CGCG	2 cut(s)	675, 1843
MwoI	GCNNNNNNNGC	7 cut(s)	620, 629, 644, 1430, 1840, 1986, 2009
NciI	CCSGG	1 cut(s)	1046
NcoI	CCATGG	2 cut(s)	639, 1947
NdeI	CATATG	2 cut(s)	387, 1060
NdeII	GATC	3 cut(s)	1087, 2188, 2329
NlaIV	GGNNCC	6 cut(s)	75, 996, 1027, 1375, 1415, 1988
NspI	RCATGY	1 cut(s)	529
OliI	CACNNNNGTG	2 cut(s)	1388, 1868
PaeR7I	CTCGAG	1 cut(s)	499
PagI	TCATGA	2 cut(s)	2113, 2371
PceI	AGGCCT	1 cut(s)	1198
PfeI	GAWTC	4 cut(s)	23, 774, 2084, 2210
PflMI	CCANNNNNTGG	1 cut(s)	401
PkrI	GCNGC	5 cut(s)	625, 646, 1264, 1717, 2178
PleI	GAGTC	3 cut(s)	140, 1049, 2343
PpsI	GAGTC	3 cut(s)	140, 1049, 2343
PpuMI	RGGWCCY	2 cut(s)	1026, 1414
PshBI	ATTAAT	1 cut(s)	807
Psp5II	RGGWCCY	2 cut(s)	1026, 1414
Psp6I	CCWGG	2 cut(s)	368, 1385
PspGI	CCWGG	2 cut(s)	368, 1385
PspN4I	GGNNCC	6 cut(s)	75, 996, 1027, 1375, 1415, 1988
PspPI	GGNCC	5 cut(s)	962, 1026, 1414, 1987, 2270
PspPPI	RGGWCCY	2 cut(s)	1026, 1414
PspXI	VCTCGAGB	1 cut(s)	499
PstI	CTGCAG	1 cut(s)	1267
PstNI	CAGNNNCTG	1 cut(s)	1434
RsaI	GTAC	8 cut(s)	742, 769, 1163, 1207, 1505, 1521, 1774, 2368
RsaNI	GTAC	8 cut(s)	741, 768, 1162, 1206, 1504, 1520, 1773, 2367
RseI	CAYNNNNRTG	4 cut(s)	1388, 1868, 2325, 2376
SatI	GCNGC	5 cut(s)	624, 645, 1263, 1716, 2177
Sau3AI	GATC	3 cut(s)	1087, 2188, 2329
Sau96I	GGNCC	5 cut(s)	962, 1026, 1414, 1987, 2270
SchI	GAGTC	3 cut(s)	140, 1050, 2344
ScrFI	CCNGG	3 cut(s)	370, 1046, 1387
SduI	GDGCHC	5 cut(s)	78, 210, 283, 291, 634
SfaNI	GCATC	2 cut(s)	610, 1249
SfcI	CTRYAG	1 cut(s)	1263
Sfr274I	CTCGAG	1 cut(s)	499
SinI	GGWCC	4 cut(s)	962, 1026, 1414, 2270
SlaI	CTCGAG	1 cut(s)	499
SmiMI	CAYNNNNRTG	4 cut(s)	1388, 1868, 2325, 2376
SmlI	CTYRAG	4 cut(s)	153, 499, 1030, 1643
SmoI	CTYRAG	4 cut(s)	153, 499, 1030, 1643
SseBI	AGGCCT	1 cut(s)	1198
SsiI	CCGC	6 cut(s)	93, 624, 673, 1142, 1715, 2273
SspI	AATATT	1 cut(s)	2121
SspMI	CTAG	7 cut(s)	540, 582, 635, 1215, 1482, 1659, 2021
StuI	AGGCCT	1 cut(s)	1198
StyD4I	CCNGG	3 cut(s)	368, 1044, 1385
StyI	CCWWGG	4 cut(s)	639, 778, 998, 1947
TaaI	ACNGT	2 cut(s)	488, 2363
TaiI	ACGT	1 cut(s)	1134
TaqI	TCGA	3 cut(s)	500, 1343, 1899
TatI	WGTACW	1 cut(s)	1519
TauI	GCSGC	2 cut(s)	626, 1718
TfiI	GAWTC	4 cut(s)	23, 774, 2084, 2210
TscAI	CASTG	6 cut(s)	210, 325, 1120, 1435, 2272, 2368
TseI	GCWGC	3 cut(s)	644, 1262, 2176
TspGWI	ACGGA	2 cut(s)	2151, 2305
TspRI	CASTG	6 cut(s)	210, 325, 1120, 1435, 2272, 2368
Van91I	CCANNNNNTGG	1 cut(s)	401
Vha464I	CTTAAG	1 cut(s)	1030
VpaK11BI	GGWCC	4 cut(s)	962, 1026, 1414, 2270
VspI	ATTAAT	1 cut(s)	807
XagI	CCTNNNNNAGG	2 cut(s)	1192, 1359
XapI	RAATTY	3 cut(s)	298, 554, 2109
XbaI	TCTAGA	1 cut(s)	1658
XceI	RCATGY	1 cut(s)	529
XhoI	CTCGAG	1 cut(s)	499
XspI	CTAG	7 cut(s)	540, 582, 635, 1215, 1482, 1659, 2021
ZraI	GACGTC	1 cut(s)	1132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.