Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000051

Physical Location & Seq

Genomic Coordinates Reverse (-)

3990941 .. 3996471

Gene Structure 5531 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1059700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGAATTCCAAACCTTCCAAGAGAGAGTTTTCAAACATGACAAAAAACCCTATGATCAAACTCGTCTTTCTTTTCTTCATATTCTTTAGGATTTTCTTCGTCGGGGCACAAAACACAACATTAATCCCAGTACATGTGGGAGTGGTTCTTGACTTGGACACCGGGTTTGGGAAAATGGGGATGAGCTGCATTGACATGGCCCTCGCCGACTTCTACGCCTCTCATGCAGGCTACAAAACCAGGCTGGTCCTACACAAAAGGGACTCTGCAGAAGATGTTGTTGTTGCAGCTACTGCAGGTGTTGATTCCCAATTGGTTCAAATGGTCGGGGTCTTCCATGTTATTTGGTCTCTAGACTTGATAAAAAATGAGAAGGTGCAAGCCATCATAGGGTTAGAATCATCAATGCAAGCCAGCTTTGTAATTGAGCTTGGAGATAAAGCTCAGGTGCCCATAATATCATTTTCCGCAACAAGCCCTTCTCTTCAGGGTTCATACTTTTTCCGAATTGCGCAGAATGACTTATCTCAAGTTAAAGCCATTAGTAGTATCATCCAAGCCTTTGGTTGGACTGAAACAGTGCCCATCAGCGTTGACAATGAGTTCGGAAAGGGAGTAATACCGTACCTGACTACTGCTTTGCAAGCAGTTGGTGCTCGTGTGCCCTACTGGAGTTTCATTCCTTCAACTGCCACGGATGAGGAAATTGTTGCAGAGCTACGCAAGTTGATGTCAATGCGAACCAGGGTCTTCATAGTGCATATGTCCCTTTTTCTTGGCTCTCGGTTTTTCTCAAAAGCAAAAGACTTTGGCATGATGGATGAAGGCTTTGTTTGGATAATGACAAATGGGATGACTAACAGTTTTAGGTCCTCGAGTCCTTCTGTCGACATAGATGACATGAAAGGGGTGTTGGGTTTAAAGACTTATGTTCCCATTACAAAAGAGCTTGAAAGTTTTAGAGCTAGATGGCAAACAAAATTCCGACAAGACAATCCAGCTATCCCTAGTGTTAAATTGGATGTTTTTGGATTGTGGGCTTATGATGCTGCTTGCGCACTAGCCATGACAGTTGAAAAGGTTGGAACAACAAACTTCCGCTCCCAGAAGATGACCGATTCTGGCAATTCCACTGGTTTGGAGAGATTAGAGGTCTCTCAAAGTGGTCCTGAACTTGTCCGAGAATTATCATGTACAAGATTCAGAGGTCTTTCAGGAGATTTCAGCCTTATTAATAGGCAACTAAAATCATCAACGTTTCAGATTATTAATGTGAACGACAATGGGGAAAGAGGGATTGGGTATTGGACACCCAAAAATGGACTTGTAAGAAACATTAAATCGAACAAACATACAAGCAGATATTCTACTTCGAAATCCAATCTTGGACCTATTATATGGCCTGGGGACACCACCTTGGTTCCAAGGGGTTGGCAGAGTAGTAAGAACGGGACCTTAAAAATTCTAATCCCGGTGAAGATAGCGTATTATGAATTTGTAACTGTAAAACATGATTCTAGAACTGGCACAACTAATGTCAGTGGATACTGCATAGATGTCTTTAACGCTTTAATAGAGGCATTGCCGTACTCAGTTGCTTATGAGTATTATCCCTTTGAGGAGCCTAATGGTGAAAGAGCTGGAAGTTACAATGATATGGTCTATCAAGTAGCTCTCGGGAACTATGATGCTGTGGTTGGAGATATTACCATTAGAGCAAATAGATCCTTGTATGTTGACTTTACATTGCCGTACAGAGAAGCGGGGGTATCAATGGTTGTGCCTGTCAAAGGAAACAATGGTGCGAAAAACACCTGGGTGTTCTTGAAGCCCTTGACCTGGGACCTTTGGGTAACCAGCTGCTGCTTTTTTATTTTCATTGGTTTTGTGGTCTGGTTTCTTGAACACCCAAAAAACAAGGACTTTCAGGGGCCTCCACATCACCAAATTGGCACAAGCTTTTGGTTCTCCTTCTCAACCATGTTTTTCGCACCTCGGGAGCTAGTGTTGAGCAACTTGGCTAGATTTGTGGTGATCGTTTGGTACTTTGTTGTTCTCATACTAACGCAAAGTTATACTGCCAGTTTAACGTCAATATTAACAGTCCAGCAGCTCCAACCAACCGTCACTGATATAAACACGCTCATTAAGAATGGGGACAAGGTTGGCTACCGACGAAATTCTTTTGTTTATGGGATTTTGAAGCAAGTAGGATTTCAGGATGATAAACTTAGGGCCTATTATTCTCGAGAAGAATTGGATAAACTTCTTCGAGACGGGAATGAAAACGGAGGTATTTCCGCTGCTTTTGATGAAACGCCCTACATGAAACTTTTTGTTTCAACTTATTGTTCAAAATATACCATTGTCGAGCAAACATTCAAAACTGATGGTTTTGCATTTGTCTTTCAAAAAGGTTCGCTTCTCACGCGTGATTTTTCAACCGCAATCACTGTAGTGCACGAGGGAGATAAAATGATGGCCATCGAAGATAAGTGGTTGAAGAAAAATGAAAGTTGTTCAAACTCCGACACCGTGAGAAGTTCCAACACTCTTAGTCTTGATAGCTTTTGGGGCCTCTTCATCGTTGCTGGTGTTGTTTCATCACTAGCAATCCTCGTATTTACAGCTATATTCCTCTATGAGCATAGCTACATCTTTACACGCGCAGATACAGAAGCCTCATTTTGGACAAGAATTCATGAAATATTAAGAGTATATTACGGAAAAGACCTGACTTCGGATATTTCTAGGAAGAGCACGCTGCAAGATAGTGATCATGGTATTGCTGCGGCTGAATCTTCACCAAACAGCAGCTGTCCCCCAAGGTCATCATCAAGCTCTCCCGACCCCACTGGACCGCACATTGTTCATCAAGAGCTGGTGGGAACTCATAATTCTTCCGTGCATGGTGGTGATCTGAGTCCCAATGGTCAAGTAACCCAAGACAGTAGTACTCATGAATATGGTATTGAGTTAACTTCCTCCCCAAGTGGTGAGCGACAGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

1004

Amino Acids

112.02

Weight (kDa)

6.85

Isoelectric Point (pI)

33.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANF_receptor	PF01094	62 - 427	4e-73	Receptor family ligand binding region
Peripla_BP_6	PF13458	119 - 365	4e-13	Periplasmic binding protein
Lig_chan-Glu_bd	PF10613	499 - 595	3e-11	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	511 - 835	1.1e-07	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	613 - 866	6.6e-39	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24710 AT2G24710 AT2G24710 AT2G24720 AT2G29100 AT2G29100 AT2G29110 AT2G29110 AT2G29120 AT5G27100
fragaria_vesca	FvH4_2g11260 FvH4_2g11280 FvH4_2g11310 FvH4_2g11310 FvH4_2g11331 FvH4_2g11331 FvH4_2g11340 FvH4_2g11340 FvH4_2g11340 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31990 FvH4_2g37140 FvH4_2g37150 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37161 FvH4_2g37161 FvH4_2g37163 FvH4_2g37163 FvH4_2g37170 FvH4_2g37181 FvH4_2g37191 FvH4_3g22781 FvH4_3g22781 FvH4_3g22781 FvH4_3g22790 FvH4_3g22790 FvH4_3g22810 FvH4_5g13460 FvH4_5g34751 FvH4_7g09130 FvH4_7g27991 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010
malus_domestica	MD08G1047600.v1.1 MD08G1047700.v1.1 MD08G1048100.v1.1 MD08G1048200.v1.1 MD08G1048300.v1.1 MD08G1048500.v1.1 MD08G1048600.v1.1 MD08G1048700.v1.1 MD08G1048900.v1.1 MD08G1049000.v1.1 MD14G1064700.v1.1 MD15G1045900.v1.1 MD15G1046000.v1.1 MD15G1046200.v1.1 MD15G1046400.v1.1 MD15G1059200.v1.1 MD15G1059500.v1.1 MD15G1059600.v1.1 MD15G1059700.v1.1
prunus_persica	Prupe.1G399500_v2.0.a1 Prupe.1G399600_v2.0.a1 Prupe.1G399700_v2.0.a1 Prupe.1G399800_v2.0.a1 Prupe.1G399900_v2.0.a1 Prupe.1G400000_v2.0.a1 Prupe.1G400300_v2.0.a1 Prupe.1G400400_v2.0.a1 Prupe.1G400500_v2.0.a1 Prupe.1G400600_v2.0.a1 Prupe.1G400700_v2.0.a1 Prupe.1G412700_v2.0.a1 Prupe.1G412800_v2.0.a1
pyrus_communis	pycom02g01350 pycom08g04020 pycom08g04040 pycom08g04080 pycom08g04090 pycom08g04100 pycom14g05240 pycom15g04300 pycom15g04310 pycom15g04330 pycom15g04340 pycom15g04380 pycom15g05560 pycom15g05570 pycom15g05580 pycom15g05600
rosa_chinensis	RchiOBHm_Chr1g0345071 RchiOBHm_Chr1g0345081 RchiOBHm_Chr1g0345171 RchiOBHm_Chr3g0480811 RchiOBHm_Chr3g0492581 RchiOBHm_Chr5g0020871 RchiOBHm_Chr5g0039801 RchiOBHm_Chr5g0039811 RchiOBHm_Chr5g0039861 RchiOBHm_Chr5g0039911 RchiOBHm_Chr5g0039941 RchiOBHm_Chr5g0039951 RchiOBHm_Chr5g0040011 RchiOBHm_Chr6g0269601 RchiOBHm_Chr6g0269621 RchiOBHm_Chr6g0269631 RchiOBHm_Chr6g0269641 RchiOBHm_Chr6g0269671 RchiOBHm_Chr6g0269681 RchiOBHm_Chr6g0269731 RchiOBHm_Chr6g0269751 RchiOBHm_Chr6g0269761 RchiOBHm_Chr6g0269771 RchiOBHm_Chr6g0269781 RchiOBHm_Chr6g0269861 RchiOBHm_Chr6g0269871 RchiOBHm_Chr6g0269881 RchiOBHm_Chr6g0269911 RchiOBHm_Chr6g0269921 RchiOBHm_Chr6g0269941 RchiOBHm_Chr6g0269951 RchiOBHm_Chr6g0304511 RchiOBHm_Chr6g0304621 RchiOBHm_Chr6g0304641 RchiOBHm_Chr6g0304651 RchiOBHm_Chr6g0304661 RchiOBHm_Chr6g0310781 RchiOBHm_Chr6g0310801 RchiOBHm_Chr6g0310811 RchiOBHm_Chr6g0310821 RchiOBHm_Chr6g0310831 RchiOBHm_Chr7g0183621 RchiOBHm_Chr7g0183651
rosa_laevigata	RLG00000005035 RLG00000005036 RLG00000010458 RLG00000010459 RLG00000010460 RLG00000010961 RLG00000010962 RLG00000010963 RLG00000010964 RLG00000010966 RLG00000010970 RLG00000013824 RLG00000013825 RLG00000013826 RLG00000013828 RLG00000013829 RLG00000013831 RLG00000013833 RLG00000013835 RLG00000013837 RLG00000013840 RLG00000013842 RLG00000013844 RLG00000022758 RLG00000023449 RLG00000026705 RLG00000028849 RLG00000028853 RLG00000028854 RLG00000032569 RLG00000033944 RLG00000033945 RLG00000033947 RLG00000033949 RLG00000033951 RLG00000034239
rosa_multiflora	Rmu_co8287891.1_g000001 Rmu_co8428745.1_g000001 Rmu_co8521767.1_g000002 Rmu_sc0000103.1_g000022 Rmu_sc0000103.1_g000031 Rmu_sc0000103.1_g000041 Rmu_sc0000103.1_g000053 Rmu_sc0000247.1_g000028 Rmu_sc0000247.1_g000051 Rmu_sc0000893.1_g000003 Rmu_sc0000893.1_g000017 Rmu_sc0001143.1_g000008 Rmu_sc0001143.1_g000019 Rmu_sc0001143.1_g000023 Rmu_sc0001975.1_g000003 Rmu_sc0001975.1_g000004 Rmu_sc0001975.1_g000005 Rmu_sc0001975.1_g000012 Rmu_sc0002151.1_g000006 Rmu_sc0003610.1_g000021 Rmu_sc0003682.1_g000001 Rmu_sc0003863.1_g000004 Rmu_sc0003863.1_g000007 Rmu_sc0003863.1_g000013 Rmu_sc0004196.1_g000005 Rmu_sc0004515.1_g000013 Rmu_sc0004515.1_g000024 Rmu_sc0004688.1_g000001 Rmu_sc0004688.1_g000003 Rmu_sc0004688.1_g000004 Rmu_sc0004748.1_g000006 Rmu_sc0004838.1_g000016 Rmu_sc0004838.1_g000017 Rmu_sc0005532.1_g000006 Rmu_sc0005532.1_g000011 Rmu_sc0005950.1_g000004 Rmu_sc0006576.1_g000005 Rmu_sc0006576.1_g000021 Rmu_sc0008520.1_g000002 Rmu_sc0008520.1_g000004 Rmu_sc0009130.1_g000002 Rmu_sc0018719.1_g000001 Rmu_sc0027713.1_g000005 Rmu_sc0040804.1_g000001 Rmu_ssc0000218.1_g000059 Rmu_ssc0000218.1_g000062 Rmu_ssc0000218.1_g000068 Rmu_ssc0000395.1_g000008 Rmu_ssc0000395.1_g000009 Rmu_ssc0000395.1_g000010 Rmu_ssc0000395.1_g000011 Rmu_ssc0000395.1_g000013 Rmu_ssc0000395.1_g000014
rosa_roxburghii	Rroxscaffold_1G00041270 Rroxscaffold_1G00041290 Rroxscaffold_1G00041300 Rroxscaffold_1G00041360 Rroxscaffold_1G00041380 Rroxscaffold_1G00057500 Rroxscaffold_3G00270350 Rroxscaffold_4G00283160 Rroxscaffold_4G00309210 Rroxscaffold_4G00309240 Rroxscaffold_4G00309260 Rroxscaffold_4G00309290 Rroxscaffold_4G00309360 Rroxscaffold_6G00392210 Rroxscaffold_6G00401030 Rroxscaffold_7G00158260 Rroxscaffold_7G00163620 Rroxscaffold_7G00163630 Rroxscaffold_7G00163650 Rroxscaffold_7G00163680 Rroxscaffold_7G00198270 Rroxscaffold_7G00198280 Rroxscaffold_7G00198330 Rroxscaffold_7G00198340 Rroxscaffold_7G00198360 Rroxscaffold_7G00198390 Rroxscaffold_7G00198410 Rroxscaffold_7G00198450 Rroxscaffold_7G00198460 Rroxscaffold_7G00198490 Rroxscaffold_7G00198500 Rroxscaffold_7G00198510 Rroxscaffold_7G00198530 Rroxscaffold_7G00198550 Rroxscaffold_7G00198600
rosa_rugosa	Rorug01G0175900 Rorug01G0176200 Rorug03G0186700 Rorug03G0254500 Rorug05G0182700 Rorug05G0183000 Rorug05G0183000 Rorug06G0049000 Rorug06G0049500 Rorug06G0049600.1 Rorug06G0049700 Rorug06G0050000 Rorug06G0050000 Rorug06G0050100 Rorug06G0050300.1 Rorug06G0050400 Rorug06G0051100 Rorug06G0051200 Rorug06G0051300 Rorug06G0051500 Rorug06G0051600 Rorug06G0051700 Rorug06G0332600 Rorug06G0332700 Rorug06G0332700 Rorug06G0383100 Rorug06G0383200 Rorug06G0383300 Rorug06G0383400 Rorug06G0459100 Rorug06G0459200
rosa_samantha	Rh1BG160400 Rh1BG160500 Rh1BG160700 Rh3DG263900 Rh3DG338400 Rh5CG164400 Rh5CG164500 Rh5CG305500 Rh5CG305900 Rh5CG306000 Rh5DG280800 Rh5DG281300 Rh6AG445300 Rh6AG497000 Rh6BG174700 Rh6BG457200 Rh6CG168900 Rh6CG169800 Rh6CG512700 Rh6CG512900 Rh6DG034100 Rh6DG160500 Rh6DG160800 Rh6DG160900 Rh6DG161100 Rh6DG161400 Rh6DG161800 Rh6DG162000 Rh6DG162100 Rh6DG162200 Rh6DG444800 Rh6DG445000 Rh6DG445100 Rh6DG445200 Rh6DG445300 Rh6DG499100 Rh6DG499200 Rh6DG499300 Rh6DG499400 Rh6DG499500 Rh6DG499700 Rh7BG059800 Rh7BG060000 Rh7BG258500 Rh7BG258700
rosa_wichuraiana	Rw0G006180 Rw0G007950 Rw0G018490 Rw0G022090 Rw1G015970 Rw1G015990 Rw3G021330 Rw5G025120 Rw5G025140 Rw5G025150 Rw5G025160 Rw5G025180 Rw5G025830 Rw5G025850 Rw6G014550 Rw6G014570 Rw6G014580 Rw6G014600 Rw6G014620 Rw6G014650 Rw6G014670 Rw6G038650 Rw6G038670 Rw6G038680 Rw6G038700 Rw6G038710 Rw6G038720 Rw6G043280 Rw6G043290 Rw7G004960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	287
Acc16I	TGCGCA	2 cut(s)	513, 1057
Acc36I	ACCTGC	1 cut(s)	287
AccB1I	GGYRCC	1 cut(s)	448
AccBSI	CCGCTC	1 cut(s)	1101
AccI	GTMKAC	1 cut(s)	888
AccII	CGCG	2 cut(s)	2431, 2667
AciI	CCGC	7 cut(s)	468, 1099, 1763, 2301, 2445, 2792, 2861
AclI	AACGTT	1 cut(s)	1256
AclWI	GGATC	1 cut(s)	1719
AcoI	YGGCCR	1 cut(s)	2481
AcsI	RAATTY	6 cut(s)	4, 980, 1461, 1493, 2179, 2697
AcuI	CTGAAG	1 cut(s)	470
AfaI	GTAC	7 cut(s)	132, 626, 1195, 1589, 1754, 2045, 2956
AfiI	CCNNNNNNNGG	8 cut(s)	168, 390, 567, 1319, 1790, 1839, 2688, 2740
AflIII	ACRYGT	2 cut(s)	133, 2429
AjnI	CCWGG	5 cut(s)	239, 743, 1402, 1814, 1838
AjuI	GAANNNNNNNTTGG	4 cut(s)	896, 928, 1281, 1313
AleI	CACNNNNGTG	2 cut(s)	1817, 2456
Alw21I	GWGCWC	3 cut(s)	658, 2463, 2762
Alw26I	GTCTC	3 cut(s)	354, 1159, 2268
Alw44I	GTGCAC	1 cut(s)	2459
AlwI	GGATC	1 cut(s)	1719
AlwNI	CAGNNNCTG	3 cut(s)	293, 1863, 2817
Ama87I	CYCGRG	4 cut(s)	874, 1676, 1995, 2247
AoxI	GGCC	6 cut(s)	198, 1400, 1931, 2235, 2481, 2575
ApaLI	GTGCAC	1 cut(s)	2459
ApoI	RAATTY	6 cut(s)	4, 980, 1461, 1493, 2179, 2697
ArsI	GACNNNNNNTTYG	6 cut(s)	149, 181, 587, 619, 622, 654
AseI	ATTAAT	3 cut(s)	122, 1233, 1269
AspLEI	GCGC	3 cut(s)	514, 1058, 2669
AsuC2I	CCSGG	2 cut(s)	163, 1472
AsuHPI	GGTGA	7 cut(s)	1486, 1643, 1934, 2044, 2796, 2927, 3008
AsuII	TTCGAA	1 cut(s)	1373
AvaI	CYCGRG	4 cut(s)	874, 1676, 1995, 2247
AvaII	GGWCC	7 cut(s)	247, 870, 1166, 1388, 1452, 1843, 2858
BaeGI	GKGCMC	5 cut(s)	109, 453, 585, 666, 2463
BalI	TGGCCA	1 cut(s)	2483
BanI	GGYRCC	1 cut(s)	448
BauI	CACGAG	2 cut(s)	657, 2462
BbsI	GAAGAC	2 cut(s)	325, 742
Bbv12I	GWGCWC	3 cut(s)	658, 2463, 2762
BccI	CCATC	7 cut(s)	392, 593, 811, 963, 2384, 2473, 2492
BceAI	ACGGC	2 cut(s)	1570, 1735
BciT130I	CCWGG	5 cut(s)	241, 745, 1404, 1816, 1840
BciVI	GTATCC	1 cut(s)	1538
BclI	TGATCA	2 cut(s)	54, 2776
BcnI	CCSGG	2 cut(s)	163, 1472
BcoDI	GTCTC	3 cut(s)	354, 1159, 2268
BfaI	CTAG	9 cut(s)	353, 966, 1008, 1061, 1518, 2003, 2022, 2609, 2751
BfmI	CTRYAG	3 cut(s)	267, 294, 2454
BfuAI	ACCTGC	1 cut(s)	287
BfuI	GTATCC	1 cut(s)	1538
BmcAI	AGTACT	1 cut(s)	2956
Bme1390I	CCNGG	7 cut(s)	163, 241, 745, 1404, 1472, 1816, 1840
Bme18I	GGWCC	7 cut(s)	247, 870, 1166, 1388, 1452, 1843, 2858
BmeT110I	CYCGRG	4 cut(s)	874, 1676, 1995, 2247
BmiI	GGNNCC	7 cut(s)	450, 1422, 1453, 1623, 1844, 1932, 2576
BmrFI	CCNGG	7 cut(s)	163, 241, 745, 1404, 1472, 1816, 1840
BmrI	ACTGGG	1 cut(s)	122
BmsI	GCATC	2 cut(s)	1036, 1678
BmuI	ACTGGG	1 cut(s)	122
BpiI	GAAGAC	2 cut(s)	325, 742
BpmI	CTGGAG	1 cut(s)	691
Bpu10I	CCTNAGC	1 cut(s)	444
Bpu14I	TTCGAA	1 cut(s)	1373
BpuEI	CTTGAG	1 cut(s)	513
BpuMI	CCSGG	2 cut(s)	163, 1472
BsaBI	GATNNNNATC	2 cut(s)	2483, 2775
BsaI	GGTCTC	2 cut(s)	354, 1159
BsaJI	CCNNGG	9 cut(s)	693, 744, 1403, 1416, 1424, 1815, 1839, 1994, 2825
BsaXI	ACNNNNNCTCC	4 cut(s)	1085, 1115, 1991, 2021
Bsc4I	CCNNNNNNNGG	8 cut(s)	168, 390, 567, 1319, 1790, 1839, 2688, 2740
Bse1I	ACTGG	6 cut(s)	128, 674, 1138, 1528, 2082, 2860
Bse3DI	GCAATG	2 cut(s)	1580, 1745
Bse8I	GATNNNNATC	2 cut(s)	2483, 2775
BseBI	CCWGG	5 cut(s)	241, 745, 1404, 1816, 1840
BseDI	CCNNGG	9 cut(s)	693, 744, 1403, 1416, 1424, 1815, 1839, 1994, 2825
BseGI	GGATG	7 cut(s)	186, 552, 703, 826, 858, 1027, 2227
BseJI	GATNNNNATC	2 cut(s)	2483, 2775
BseLI	CCNNNNNNNGG	8 cut(s)	168, 390, 567, 1319, 1790, 1839, 2688, 2740
BseMI	GCAATG	2 cut(s)	1580, 1745
BseMII	CTCAG	3 cut(s)	458, 1605, 2912
BseNI	ACTGG	6 cut(s)	128, 674, 1138, 1528, 2082, 2860
BseRI	GAGGAG	1 cut(s)	1634
BseSI	GKGCMC	5 cut(s)	109, 453, 585, 666, 2463
Bsh1236I	CGCG	2 cut(s)	2431, 2667
BshFI	GGCC	6 cut(s)	200, 1402, 1933, 2237, 2483, 2577
BshNI	GGYRCC	1 cut(s)	448
BsiHKAI	GWGCWC	3 cut(s)	658, 2463, 2762
BsiHKCI	CYCGRG	4 cut(s)	874, 1676, 1995, 2247
BsiSI	CCGG	2 cut(s)	162, 1472
BslFI	GGGAC	8 cut(s)	275, 751, 1421, 1465, 1856, 2171, 2805, 2910
BslI	CCNNNNNNNGG	8 cut(s)	168, 390, 567, 1319, 1790, 1839, 2688, 2740
BsmAI	GTCTC	3 cut(s)	354, 1159, 2268
BsmBI	CGTCTC	1 cut(s)	2268
BsmFI	GGGAC	8 cut(s)	275, 751, 1421, 1465, 1856, 2171, 2805, 2910
BsnI	GGCC	6 cut(s)	200, 1402, 1933, 2237, 2483, 2577
Bso31I	GGTCTC	2 cut(s)	354, 1159
BsoBI	CYCGRG	4 cut(s)	874, 1676, 1995, 2247
Bsp119I	TTCGAA	1 cut(s)	1373
Bsp1286I	GDGCHC	7 cut(s)	109, 453, 585, 658, 666, 2463, 2762
Bsp1407I	TGTACA	1 cut(s)	1193
Bsp143I	GATC	5 cut(s)	54, 1724, 2034, 2776, 2917
BspACI	CCGC	7 cut(s)	468, 1099, 1763, 2301, 2445, 2792, 2861
BspANI	GGCC	6 cut(s)	200, 1402, 1933, 2237, 2483, 2577
BspCNI	CTCAG	3 cut(s)	457, 1604, 2913
BspFNI	CGCG	2 cut(s)	2431, 2667
BspHI	TCATGA	2 cut(s)	2701, 2959
BspLI	GGNNCC	7 cut(s)	450, 1422, 1453, 1623, 1844, 1932, 2576
BspMAI	CTGCAG	2 cut(s)	271, 298
BspMI	ACCTGC	1 cut(s)	287
BspPI	GGATC	1 cut(s)	1719
BspQI	GCTCTTC	1 cut(s)	2750
BspT104I	TTCGAA	1 cut(s)	1373
BspT107I	GGYRCC	1 cut(s)	448
BspTNI	GGTCTC	2 cut(s)	354, 1159
BsrBI	CCGCTC	1 cut(s)	1101
BsrDI	GCAATG	2 cut(s)	1580, 1745
BsrGI	TGTACA	1 cut(s)	1193
BsrI	ACTGG	6 cut(s)	128, 674, 1138, 1528, 2082, 2860
BssECI	CCNNGG	9 cut(s)	693, 744, 1403, 1416, 1424, 1815, 1839, 1994, 2825
BssMI	GATC	5 cut(s)	54, 1724, 2034, 2776, 2917
BssSI	CACGAG	2 cut(s)	657, 2462
BssT1I	CCWWGG	3 cut(s)	1416, 1424, 2825
Bst2BI	CACGAG	2 cut(s)	657, 2462
Bst2UI	CCWGG	5 cut(s)	241, 745, 1404, 1816, 1840
Bst6I	CTCTTC	3 cut(s)	489, 2585, 2750
BstAPI	GCANNNNNTGC	2 cut(s)	293, 653
BstAUI	TGTACA	1 cut(s)	1193
BstBI	TTCGAA	1 cut(s)	1373
BstC8I	GCNNGC	7 cut(s)	229, 381, 411, 415, 645, 1054, 2762
BstDEI	CTNAG	5 cut(s)	444, 1591, 2231, 2555, 2921
BstDSI	CCRYGG	1 cut(s)	693
BstEII	GGTNACC	1 cut(s)	1852
BstENI	CCTNNNNNAGG	1 cut(s)	1788
BstF5I	GGATG	7 cut(s)	186, 552, 703, 826, 858, 1027, 2227
BstFNI	CGCG	2 cut(s)	2431, 2667
BstHHI	GCGC	3 cut(s)	514, 1058, 2669
BstKTI	GATC	5 cut(s)	57, 1727, 2037, 2779, 2920
BstMAI	GTCTC	3 cut(s)	354, 1159, 2268
BstMBI	GATC	5 cut(s)	54, 1724, 2034, 2776, 2917
BstMWI	GCNNNNNNNGC	8 cut(s)	224, 293, 644, 653, 1046, 1055, 2428, 2574
BstNI	CCWGG	5 cut(s)	241, 745, 1404, 1816, 1840
BstNSI	RCATGY	1 cut(s)	137
BstPI	GGTNACC	1 cut(s)	1852
BstSCI	CCNGG	7 cut(s)	161, 239, 743, 1402, 1470, 1814, 1838
BstSFI	CTRYAG	3 cut(s)	267, 294, 2454
BstSLI	GKGCMC	5 cut(s)	109, 453, 585, 666, 2463
BstUI	CGCG	2 cut(s)	2431, 2667
BstV2I	GAAGAC	2 cut(s)	325, 742
BstX2I	RGATCY	1 cut(s)	1724
BstXI	CCANNNNNNTGG	3 cut(s)	563, 1138, 1431
BstYI	RGATCY	1 cut(s)	1724
BsuI	GTATCC	1 cut(s)	1538
BsuRI	GGCC	6 cut(s)	200, 1402, 1933, 2237, 2483, 2577
BtgI	CCRYGG	1 cut(s)	693
BtsCI	GGATG	7 cut(s)	186, 552, 703, 826, 858, 1027, 2227
BtsIMutI	CAGTG	6 cut(s)	585, 1131, 1546, 2127, 2451, 2853
BveI	ACCTGC	1 cut(s)	287
Cac8I	GCNNGC	7 cut(s)	229, 381, 411, 415, 645, 1054, 2762
CaiI	CAGNNNCTG	3 cut(s)	293, 1863, 2817
CciI	TCATGA	2 cut(s)	2701, 2959
CfoI	GCGC	3 cut(s)	514, 1058, 2669
Csp6I	GTAC	7 cut(s)	131, 625, 1194, 1588, 1753, 2044, 2955
CviQI	GTAC	7 cut(s)	131, 625, 1194, 1588, 1753, 2044, 2955
DdeI	CTNAG	5 cut(s)	444, 1591, 2231, 2555, 2921
DpnI	GATC	5 cut(s)	56, 1726, 2036, 2778, 2919
DpnII	GATC	5 cut(s)	54, 1724, 2034, 2776, 2917
DraI	TTTAAA	1 cut(s)	921
EaeI	YGGCCR	1 cut(s)	2481
Eam1104I	CTCTTC	3 cut(s)	489, 2585, 2750
EarI	CTCTTC	3 cut(s)	489, 2585, 2750
Eco130I	CCWWGG	3 cut(s)	1416, 1424, 2825
Eco31I	GGTCTC	2 cut(s)	354, 1159
Eco47I	GGWCC	7 cut(s)	247, 870, 1166, 1388, 1452, 1843, 2858
Eco57I	CTGAAG	1 cut(s)	470
Eco88I	CYCGRG	4 cut(s)	874, 1676, 1995, 2247
Eco91I	GGTNACC	1 cut(s)	1852
EcoNI	CCTNNNNNAGG	1 cut(s)	1788
EcoO109I	RGGNCCY	6 cut(s)	870, 1452, 1843, 1931, 2235, 2575
EcoO65I	GGTNACC	1 cut(s)	1852
EcoRI	GAATTC	2 cut(s)	4, 2697
EcoRII	CCWGG	5 cut(s)	239, 743, 1402, 1814, 1838
EcoT14I	CCWWGG	3 cut(s)	1416, 1424, 2825
ErhI	CCWWGG	3 cut(s)	1416, 1424, 2825
Esp3I	CGTCTC	1 cut(s)	2268
FalI	AAGNNNNNCTT	2 cut(s)	2406, 2438
FaqI	GGGAC	8 cut(s)	275, 751, 1421, 1465, 1856, 2171, 2805, 2910
FauI	CCCGC	1 cut(s)	1756
FauNDI	CATATG	1 cut(s)	762
FbaI	TGATCA	2 cut(s)	54, 2776
FblI	GTMKAC	1 cut(s)	888
FokI	GGATG	7 cut(s)	193, 539, 710, 833, 865, 1034, 2234
FspBI	CTAG	9 cut(s)	353, 966, 1008, 1061, 1518, 2003, 2022, 2609, 2751
FspI	TGCGCA	2 cut(s)	513, 1057
GlaI	GCGC	3 cut(s)	513, 1057, 2668
GsuI	CTGGAG	1 cut(s)	691
HaeIII	GGCC	6 cut(s)	200, 1402, 1933, 2237, 2483, 2577
HapII	CCGG	2 cut(s)	162, 1472
HhaI	GCGC	3 cut(s)	514, 1058, 2669
Hin6I	GCGC	3 cut(s)	512, 1056, 2667
HinP1I	GCGC	3 cut(s)	512, 1056, 2667
HincII	GTYRAC	4 cut(s)	595, 889, 1738, 2979
HindII	GTYRAC	4 cut(s)	595, 889, 1738, 2979
HindIII	AAGCTT	1 cut(s)	1957
HinfI	GANTC	9 cut(s)	263, 305, 398, 877, 1118, 1200, 1514, 2798, 2923
HpaI	GTTAAC	1 cut(s)	2979
HpaII	CCGG	2 cut(s)	162, 1472
HphI	GGTGA	7 cut(s)	1486, 1643, 1934, 2044, 2796, 2927, 3008
Hpy166II	GTNNAC	6 cut(s)	595, 889, 1276, 1738, 2461, 2979
Hpy188I	TCNGA	9 cut(s)	506, 608, 986, 1181, 1205, 1263, 2530, 2743, 2922
Hpy8I	GTNNAC	6 cut(s)	595, 889, 1276, 1738, 2461, 2979
Hpy99I	CGWCG	2 cut(s)	104, 2178
HpyAV	CCTTC	7 cut(s)	24, 367, 489, 693, 818, 891, 1981
HpyCH4IV	ACGT	2 cut(s)	1256, 2090
HpyF10VI	GCNNNNNNNGC	8 cut(s)	224, 293, 644, 653, 1046, 1055, 2428, 2574
HpyF3I	CTNAG	5 cut(s)	444, 1591, 2231, 2555, 2921
HpySE526I	ACGT	2 cut(s)	1256, 2090
HspAI	GCGC	3 cut(s)	512, 1056, 2667
Ksp22I	TGATCA	2 cut(s)	54, 2776
KspAI	GTTAAC	1 cut(s)	2979
Kzo9I	GATC	5 cut(s)	54, 1724, 2034, 2776, 2917
LguI	GCTCTTC	1 cut(s)	2750
LmnI	GCTCC	4 cut(s)	1106, 1621, 1999, 2118
LweI	GCATC	2 cut(s)	1036, 1678
MaeI	CTAG	9 cut(s)	353, 966, 1008, 1061, 1518, 2003, 2022, 2609, 2751
MaeII	ACGT	2 cut(s)	1256, 2090
MaeIII	GTNAC	5 cut(s)	1498, 1646, 1852, 2125, 2938
MalI	GATC	5 cut(s)	56, 1726, 2036, 2778, 2919
MbiI	CCGCTC	1 cut(s)	1101
MboI	GATC	5 cut(s)	54, 1724, 2034, 2776, 2917
MfeI	CAATTG	1 cut(s)	311
MflI	RGATCY	1 cut(s)	1724
MhlI	GDGCHC	7 cut(s)	109, 453, 585, 658, 666, 2463, 2762
MlsI	TGGCCA	1 cut(s)	2483
MluI	ACGCGT	1 cut(s)	2429
MluNI	TGGCCA	1 cut(s)	2483
MlyI	GAGTC	3 cut(s)	257, 886, 2932
MmeI	TCCRAC	7 cut(s)	548, 1009, 1063, 1678, 2140, 2553, 2571
Mox20I	TGGCCA	1 cut(s)	2483
MscI	TGGCCA	1 cut(s)	2483
MslI	CAYNNNNRTG	5 cut(s)	194, 1817, 2456, 2913, 2964
Msp20I	TGGCCA	1 cut(s)	2483
MspA1I	CMGCKG	3 cut(s)	1860, 2303, 2817
MspI	CCGG	2 cut(s)	162, 1472
MspR9I	CCNGG	7 cut(s)	163, 241, 745, 1404, 1472, 1816, 1840
MunI	CAATTG	1 cut(s)	311
MvaI	CCWGG	5 cut(s)	241, 745, 1404, 1816, 1840
MvnI	CGCG	2 cut(s)	2431, 2667
MwoI	GCNNNNNNNGC	8 cut(s)	224, 293, 644, 653, 1046, 1055, 2428, 2574
NciI	CCSGG	2 cut(s)	163, 1472
NdeI	CATATG	1 cut(s)	762
NdeII	GATC	5 cut(s)	54, 1724, 2034, 2776, 2917
NlaIV	GGNNCC	7 cut(s)	450, 1422, 1453, 1623, 1844, 1932, 2576
NmuCI	GTSAC	1 cut(s)	2125
NsbI	TGCGCA	2 cut(s)	513, 1057
NspI	RCATGY	1 cut(s)	137
NspV	TTCGAA	1 cut(s)	1373
OliI	CACNNNNGTG	2 cut(s)	1817, 2456
PaeR7I	CTCGAG	2 cut(s)	874, 2247
PagI	TCATGA	2 cut(s)	2701, 2959
PaqCI	CACCTGC	1 cut(s)	287
PciI	ACATGT	1 cut(s)	133
PciSI	GCTCTTC	1 cut(s)	2750
PfeI	GAWTC	6 cut(s)	305, 398, 1118, 1200, 1514, 2798
PleI	GAGTC	3 cut(s)	257, 885, 2931
PpsI	GAGTC	3 cut(s)	257, 885, 2931
PpuMI	RGGWCCY	3 cut(s)	870, 1452, 1843
PscI	ACATGT	1 cut(s)	133
PshBI	ATTAAT	3 cut(s)	122, 1233, 1269
Psp1406I	AACGTT	1 cut(s)	1256
Psp5II	RGGWCCY	3 cut(s)	870, 1452, 1843
Psp6I	CCWGG	5 cut(s)	239, 743, 1402, 1814, 1838
PspEI	GGTNACC	1 cut(s)	1852
PspGI	CCWGG	5 cut(s)	239, 743, 1402, 1814, 1838
PspN4I	GGNNCC	7 cut(s)	450, 1422, 1453, 1623, 1844, 1932, 2576
PspPPI	RGGWCCY	3 cut(s)	870, 1452, 1843
PspXI	VCTCGAGB	1 cut(s)	874
PstI	CTGCAG	2 cut(s)	271, 298
PstNI	CAGNNNCTG	3 cut(s)	293, 1863, 2817
PsuI	RGATCY	1 cut(s)	1724
PvuII	CAGCTG	2 cut(s)	1860, 2817
RsaI	GTAC	7 cut(s)	132, 626, 1195, 1589, 1754, 2045, 2956
RsaNI	GTAC	7 cut(s)	131, 625, 1194, 1588, 1753, 2044, 2955
RseI	CAYNNNNRTG	5 cut(s)	194, 1817, 2456, 2913, 2964
SalI	GTCGAC	1 cut(s)	887
SapI	GCTCTTC	1 cut(s)	2750
Sau3AI	GATC	5 cut(s)	54, 1724, 2034, 2776, 2917
ScaI	AGTACT	1 cut(s)	2956
SchI	GAGTC	3 cut(s)	257, 886, 2932
ScrFI	CCNGG	7 cut(s)	163, 241, 745, 1404, 1472, 1816, 1840
SduI	GDGCHC	7 cut(s)	109, 453, 585, 658, 666, 2463, 2762
SfaNI	GCATC	2 cut(s)	1036, 1678
SfcI	CTRYAG	3 cut(s)	267, 294, 2454
Sfr274I	CTCGAG	2 cut(s)	874, 2247
SfuI	TTCGAA	1 cut(s)	1373
SinI	GGWCC	7 cut(s)	247, 870, 1166, 1388, 1452, 1843, 2858
SlaI	CTCGAG	2 cut(s)	874, 2247
SmiMI	CAYNNNNRTG	5 cut(s)	194, 1817, 2456, 2913, 2964
SmlI	CTYRAG	3 cut(s)	528, 874, 2247
SmoI	CTYRAG	3 cut(s)	528, 874, 2247
SsiI	CCGC	7 cut(s)	468, 1099, 1763, 2301, 2445, 2792, 2861
SspI	AATATT	2 cut(s)	2097, 2709
SspMI	CTAG	9 cut(s)	353, 966, 1008, 1061, 1518, 2003, 2022, 2609, 2751
StyD4I	CCNGG	7 cut(s)	161, 239, 743, 1402, 1470, 1814, 1838
StyI	CCWWGG	3 cut(s)	1416, 1424, 2825
TaiI	ACGT	2 cut(s)	1259, 2093
TaqI	TCGA	8 cut(s)	875, 888, 1343, 1373, 2248, 2272, 2370, 2487
TaqII	GACCGA	1 cut(s)	1130
TatI	WGTACW	3 cut(s)	130, 1193, 2954
TauI	GCSGC	1 cut(s)	2795
TfiI	GAWTC	6 cut(s)	305, 398, 1118, 1200, 1514, 2798
TscAI	CASTG	6 cut(s)	585, 1138, 1546, 2134, 2458, 2860
TseFI	GTSAC	1 cut(s)	2125
Tsp45I	GTSAC	1 cut(s)	2125
TspGWI	ACGGA	4 cut(s)	710, 2304, 2739, 2893
TspRI	CASTG	6 cut(s)	585, 1138, 1546, 2134, 2458, 2860
VneI	GTGCAC	1 cut(s)	2459
VpaK11BI	GGWCC	7 cut(s)	247, 870, 1166, 1388, 1452, 1843, 2858
VspI	ATTAAT	3 cut(s)	122, 1233, 1269
XagI	CCTNNNNNAGG	1 cut(s)	1788
XapI	RAATTY	6 cut(s)	4, 980, 1461, 1493, 2179, 2697
XbaI	TCTAGA	2 cut(s)	352, 1517
XceI	RCATGY	1 cut(s)	137
XhoI	CTCGAG	2 cut(s)	874, 2247
XmiI	GTMKAC	1 cut(s)	888
XspI	CTAG	9 cut(s)	353, 966, 1008, 1061, 1518, 2003, 2022, 2609, 2751
ZrmI	AGTACT	1 cut(s)	2956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.