MD08G1048900.v1.1

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000051
Physical Location & Seq
Reverse (-)
3806490 .. 3808770
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1048900.v1.1.491

Sequence Viewer

Length: 1971 bp
ATGAAAAGGAGGACACGAACCAACCTGGTTTGTTCTTTTTTCTTGCTTATTTCTTTAAGCATTAATCTTTCCCTGGTCATGGGGCAGAAGTCACAGAACACAACAATCCCAGTGAAAGTTGGTGTGGTTCTTGATGACCTTGATTCATCAAGTACAAAAGTTTGGTTGAGCTGCATCGAAATGGCCCTCTCCGACTTTTATACCTCCCGTGCTTCCTCTTCAACTAGGCTGGCCTTGAGCATTAGAGACTCGAGAGAAGACGTTGTCGATGCAGCTGCTGCAGCTTTAGACCTGATAAAGAATCTTCAAGTGCAAGCAATCTTAGGACCGAAATCATCGATGCAAGCCAACTTTGTAATTGATCTTGGAGAGGAAGCCCAAGTGCCCATAATGTCATTTTCAGCTACAAGTCCTTCCCTCACTTCAATTCGGAGTCCATATTTTTTCCGAGCTGTGCAAAATGATTCCTCTCAAGTCAAAGCTATAAGTGCTATCATCGAGGCCTTTGGATGGAGAGAAGCGGTGCCAATCTATGTGGACAACGAATACGGTGAGGGAATAATACCTTACTTGGCCGATGCCTTGCAAGAAGTTGAAGCTCGTATCCCGTATCGAAGTGTCATACCTCCAGAGGCCAGTGATGTTCAAATTGAGGCAGAGCTTTACAAGTTAATGACGATGCAAACTAGAGTCTTCATTGTGCACATGTTGCCCTCTCTTGGTAATCGCCTTTTCGCCAAGGCGCAAGAGATGGGAATGATGGATGAAGGCTATGTTTGGATCATGACTGGTGGGCTAACCAATCATATAACAGCAATGAGTTCTTCAGTGAGGACTTATGTGATGGAAGGTGCATTGGGCGTGAGGACTTATGTGCCCCAAACGGTGGAGCTTAAGGATTTTAGAGTTCGATGGAAAAAGAAATTTCAAATGGAAAATCCAAGCATAATTGATGCTGAACTAGATGTTCCTGCACTCCGGGCTTATGATGCTGCTTTTGCATTAGCCATGGCAGTTGAAAATGTCGGGACTAGGAACTTTGGCTTCGAAAAGATCAATGGTTCCACCAACTCATCAACTGATCTTGAAGCTTTGGGGCTCTCTCAAAATGGTCCAGAACTTTGCAAGTCCTTGGCAAGTACTAAATTTATAGGCCTCGCTGGGGATTTCAGTTTTGTTGATGGACAATTACAACCTTCCATCTTTGAAATGGTGAATGTTATTGGTCATGCAGCAAAACCAATTGGGTTTTGGACACCACAGGGTGGACTTGTGAAAAAATTGAAATTCACCAACACAGAAAATACTACATATTCAACTTCTAAATCGAATCTGGGACCTATTTTATGGCCAGGAGATTCTACCTCGGTTCCCAAGGGATGGGAGAACCCGACATATGGCAAAAGGTTGAGAGTAGGAGTTCCCGTAATTGATGGTTTTGCTGATTTTGTGAGGGTGGTACCTGATCCTAGCAACAATACAACCGAAGTTTCCGGGTTCTGTATCGACGTCTTTGATGCTGCAATGCGAAAATTGCCATATGCTGTTTCTTATGACTTGATTCCCTTTGCAAAGCCTGATGGTACAAGTGCTGGCACTTACAGTGATTTGGTCCATCAAGTTTTTCTTGGGAAATTCGACGCGTTGGCAGCGGACACAACAATTAGAGCAAACAGGTCTATGTATGTGGACTTCACATTGCCATACACGGAATCAGGAGTGGTAATGGTAGTGCCAATGAAAGACGGCAAGAGCAAAAATGCATGGATCTTCTTGAAGCCTTTGACATGGGACCTCTGGCTGACAAGTTCTTGCTTTTTTATCTTCATTGGTTTTGTGGTTTGGGTTCTTGAACACCGTATTAACGAAGAATTTCGAGGGCCTCCCTTGCATCAAGTCGGGACGAGCTTTTGGTTCTCCTTCTCGACCATGGTTTTTGCACACCGTAAATACTACTATACAACTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

657

Amino Acids

72.73

Weight (kDa)

5.81

Isoelectric Point (pI)

33.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 37 - 344 2.8e-19 Periplasmic binding protein
ANF_receptor PF01094 56 - 408 2.2e-81 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 492 - 579 6.5e-08 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 493 - 583 1e-05 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 596 - 649 8.7e-10 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24710 AT2G24710 AT2G24710 AT2G24720 AT2G29100 AT2G29100 AT2G29110 AT2G29110 AT2G29120 AT5G27100
fragaria_vesca FvH4_2g11260 FvH4_2g11280 FvH4_2g11310 FvH4_2g11310 FvH4_2g11331 FvH4_2g11331 FvH4_2g11340 FvH4_2g11340 FvH4_2g11340 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31990 FvH4_2g37140 FvH4_2g37150 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37161 FvH4_2g37161 FvH4_2g37163 FvH4_2g37163 FvH4_2g37170 FvH4_2g37181 FvH4_2g37191 FvH4_3g22781 FvH4_3g22781 FvH4_3g22781 FvH4_3g22790 FvH4_3g22790 FvH4_3g22810 FvH4_5g13460 FvH4_5g34751 FvH4_7g09130 FvH4_7g27991 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010
malus_domestica MD08G1047600.v1.1 MD08G1047700.v1.1 MD08G1048100.v1.1 MD08G1048200.v1.1 MD08G1048300.v1.1 MD08G1048500.v1.1 MD08G1048600.v1.1 MD08G1048700.v1.1 MD08G1048900.v1.1 MD08G1049000.v1.1 MD14G1064700.v1.1 MD15G1045900.v1.1 MD15G1046000.v1.1 MD15G1046200.v1.1 MD15G1046400.v1.1 MD15G1059200.v1.1 MD15G1059500.v1.1 MD15G1059600.v1.1 MD15G1059700.v1.1
prunus_persica Prupe.1G399500_v2.0.a1 Prupe.1G399600_v2.0.a1 Prupe.1G399700_v2.0.a1 Prupe.1G399800_v2.0.a1 Prupe.1G399900_v2.0.a1 Prupe.1G400000_v2.0.a1 Prupe.1G400300_v2.0.a1 Prupe.1G400400_v2.0.a1 Prupe.1G400500_v2.0.a1 Prupe.1G400600_v2.0.a1 Prupe.1G400700_v2.0.a1 Prupe.1G412700_v2.0.a1 Prupe.1G412800_v2.0.a1
pyrus_communis pycom02g01350 pycom08g04020 pycom08g04040 pycom08g04080 pycom08g04090 pycom08g04100 pycom14g05240 pycom15g04300 pycom15g04310 pycom15g04330 pycom15g04340 pycom15g04380 pycom15g05560 pycom15g05570 pycom15g05580 pycom15g05600
rosa_chinensis RchiOBHm_Chr1g0345071 RchiOBHm_Chr1g0345081 RchiOBHm_Chr1g0345171 RchiOBHm_Chr3g0480811 RchiOBHm_Chr3g0492581 RchiOBHm_Chr5g0020871 RchiOBHm_Chr5g0039801 RchiOBHm_Chr5g0039811 RchiOBHm_Chr5g0039861 RchiOBHm_Chr5g0039911 RchiOBHm_Chr5g0039941 RchiOBHm_Chr5g0039951 RchiOBHm_Chr5g0040011 RchiOBHm_Chr6g0269601 RchiOBHm_Chr6g0269621 RchiOBHm_Chr6g0269631 RchiOBHm_Chr6g0269641 RchiOBHm_Chr6g0269671 RchiOBHm_Chr6g0269681 RchiOBHm_Chr6g0269731 RchiOBHm_Chr6g0269751 RchiOBHm_Chr6g0269761 RchiOBHm_Chr6g0269771 RchiOBHm_Chr6g0269781 RchiOBHm_Chr6g0269861 RchiOBHm_Chr6g0269871 RchiOBHm_Chr6g0269881 RchiOBHm_Chr6g0269911 RchiOBHm_Chr6g0269921 RchiOBHm_Chr6g0269941 RchiOBHm_Chr6g0269951 RchiOBHm_Chr6g0304511 RchiOBHm_Chr6g0304621 RchiOBHm_Chr6g0304641 RchiOBHm_Chr6g0304651 RchiOBHm_Chr6g0304661 RchiOBHm_Chr6g0310781 RchiOBHm_Chr6g0310801 RchiOBHm_Chr6g0310811 RchiOBHm_Chr6g0310821 RchiOBHm_Chr6g0310831 RchiOBHm_Chr7g0183621 RchiOBHm_Chr7g0183651
rosa_laevigata RLG00000005035 RLG00000005036 RLG00000010458 RLG00000010459 RLG00000010460 RLG00000010961 RLG00000010962 RLG00000010963 RLG00000010964 RLG00000010966 RLG00000010970 RLG00000013824 RLG00000013825 RLG00000013826 RLG00000013828 RLG00000013829 RLG00000013831 RLG00000013833 RLG00000013835 RLG00000013837 RLG00000013840 RLG00000013842 RLG00000013844 RLG00000022758 RLG00000023449 RLG00000026705 RLG00000028849 RLG00000028853 RLG00000028854 RLG00000032569 RLG00000033944 RLG00000033945 RLG00000033947 RLG00000033949 RLG00000033951 RLG00000034239
rosa_multiflora Rmu_co8287891.1_g000001 Rmu_co8428745.1_g000001 Rmu_co8521767.1_g000002 Rmu_sc0000103.1_g000022 Rmu_sc0000103.1_g000031 Rmu_sc0000103.1_g000041 Rmu_sc0000103.1_g000053 Rmu_sc0000247.1_g000028 Rmu_sc0000247.1_g000051 Rmu_sc0000893.1_g000003 Rmu_sc0000893.1_g000017 Rmu_sc0001143.1_g000008 Rmu_sc0001143.1_g000019 Rmu_sc0001143.1_g000023 Rmu_sc0001975.1_g000003 Rmu_sc0001975.1_g000004 Rmu_sc0001975.1_g000005 Rmu_sc0001975.1_g000012 Rmu_sc0002151.1_g000006 Rmu_sc0003610.1_g000021 Rmu_sc0003682.1_g000001 Rmu_sc0003863.1_g000004 Rmu_sc0003863.1_g000007 Rmu_sc0003863.1_g000013 Rmu_sc0004196.1_g000005 Rmu_sc0004515.1_g000013 Rmu_sc0004515.1_g000024 Rmu_sc0004688.1_g000001 Rmu_sc0004688.1_g000003 Rmu_sc0004688.1_g000004 Rmu_sc0004748.1_g000006 Rmu_sc0004838.1_g000016 Rmu_sc0004838.1_g000017 Rmu_sc0005532.1_g000006 Rmu_sc0005532.1_g000011 Rmu_sc0005950.1_g000004 Rmu_sc0006576.1_g000005 Rmu_sc0006576.1_g000021 Rmu_sc0008520.1_g000002 Rmu_sc0008520.1_g000004 Rmu_sc0009130.1_g000002 Rmu_sc0018719.1_g000001 Rmu_sc0027713.1_g000005 Rmu_sc0040804.1_g000001 Rmu_ssc0000218.1_g000059 Rmu_ssc0000218.1_g000062 Rmu_ssc0000218.1_g000068 Rmu_ssc0000395.1_g000008 Rmu_ssc0000395.1_g000009 Rmu_ssc0000395.1_g000010 Rmu_ssc0000395.1_g000011 Rmu_ssc0000395.1_g000013 Rmu_ssc0000395.1_g000014
rosa_roxburghii Rroxscaffold_1G00041270 Rroxscaffold_1G00041290 Rroxscaffold_1G00041300 Rroxscaffold_1G00041360 Rroxscaffold_1G00041380 Rroxscaffold_1G00057500 Rroxscaffold_3G00270350 Rroxscaffold_4G00283160 Rroxscaffold_4G00309210 Rroxscaffold_4G00309240 Rroxscaffold_4G00309260 Rroxscaffold_4G00309290 Rroxscaffold_4G00309360 Rroxscaffold_6G00392210 Rroxscaffold_6G00401030 Rroxscaffold_7G00158260 Rroxscaffold_7G00163620 Rroxscaffold_7G00163630 Rroxscaffold_7G00163650 Rroxscaffold_7G00163680 Rroxscaffold_7G00198270 Rroxscaffold_7G00198280 Rroxscaffold_7G00198330 Rroxscaffold_7G00198340 Rroxscaffold_7G00198360 Rroxscaffold_7G00198390 Rroxscaffold_7G00198410 Rroxscaffold_7G00198450 Rroxscaffold_7G00198460 Rroxscaffold_7G00198490 Rroxscaffold_7G00198500 Rroxscaffold_7G00198510 Rroxscaffold_7G00198530 Rroxscaffold_7G00198550 Rroxscaffold_7G00198600
rosa_rugosa Rorug01G0175900 Rorug01G0176200 Rorug03G0186700 Rorug03G0254500 Rorug05G0182700 Rorug05G0183000 Rorug05G0183000 Rorug06G0049000 Rorug06G0049500 Rorug06G0049600.1 Rorug06G0049700 Rorug06G0050000 Rorug06G0050000 Rorug06G0050100 Rorug06G0050300.1 Rorug06G0050400 Rorug06G0051100 Rorug06G0051200 Rorug06G0051300 Rorug06G0051500 Rorug06G0051600 Rorug06G0051700 Rorug06G0332600 Rorug06G0332700 Rorug06G0332700 Rorug06G0383100 Rorug06G0383200 Rorug06G0383300 Rorug06G0383400 Rorug06G0459100 Rorug06G0459200
rosa_samantha Rh1BG160400 Rh1BG160500 Rh1BG160700 Rh3DG263900 Rh3DG338400 Rh5CG164400 Rh5CG164500 Rh5CG305500 Rh5CG305900 Rh5CG306000 Rh5DG280800 Rh5DG281300 Rh6AG445300 Rh6AG497000 Rh6BG174700 Rh6BG457200 Rh6CG168900 Rh6CG169800 Rh6CG512700 Rh6CG512900 Rh6DG034100 Rh6DG160500 Rh6DG160800 Rh6DG160900 Rh6DG161100 Rh6DG161400 Rh6DG161800 Rh6DG162000 Rh6DG162100 Rh6DG162200 Rh6DG444800 Rh6DG445000 Rh6DG445100 Rh6DG445200 Rh6DG445300 Rh6DG499100 Rh6DG499200 Rh6DG499300 Rh6DG499400 Rh6DG499500 Rh6DG499700 Rh7BG059800 Rh7BG060000 Rh7BG258500 Rh7BG258700
rosa_wichuraiana Rw0G006180 Rw0G007950 Rw0G018490 Rw0G022090 Rw1G015970 Rw1G015990 Rw3G021330 Rw5G025120 Rw5G025140 Rw5G025150 Rw5G025160 Rw5G025180 Rw5G025830 Rw5G025850 Rw6G014550 Rw6G014570 Rw6G014580 Rw6G014600 Rw6G014620 Rw6G014650 Rw6G014670 Rw6G038650 Rw6G038670 Rw6G038680 Rw6G038700 Rw6G038710 Rw6G038720 Rw6G043280 Rw6G043290 Rw7G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1512
Acc65I GGTACC 1 cut(s) 1459
AccB1I GGYRCC 2 cut(s) 523, 1459
AccB7I CCANNNNNTGG 3 cut(s) 886, 1265, 1380
AccII CGCG 1 cut(s) 1643
AciI CCGC 2 cut(s) 521, 1652
AclWI GGATC 3 cut(s) 788, 1460, 1775
AcoI YGGCCR 2 cut(s) 573, 1349
AcsI RAATTY 5 cut(s) 923, 1145, 1286, 1634, 1871
AcuI CTGAAG 1 cut(s) 810
AcyI GRCGYC 1 cut(s) 1509
AdeI CACNNNGTG 1 cut(s) 1265
AfaI GTAC 4 cut(s) 154, 1141, 1461, 1585
AfiI CCNNNNNNNGG 8 cut(s) 79, 510, 719, 886, 1162, 1265, 1380, 1397
AflII CTTAAG 1 cut(s) 893
AflIII ACRYGT 2 cut(s) 705, 1641
AjnI CCWGG 3 cut(s) 24, 72, 1351
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 1 cut(s) 240
Alw44I GTGCAC 1 cut(s) 701
AlwI GGATC 3 cut(s) 788, 1460, 1775
AlwNI CAGNNNCTG 1 cut(s) 278
Ama87I CYCGRG 1 cut(s) 250
AoxI GGCC 8 cut(s) 183, 231, 501, 573, 633, 1153, 1349, 1880
ApaLI GTGCAC 1 cut(s) 701
ApeKI GCWGC 9 cut(s) 171, 272, 275, 278, 281, 992, 1232, 1520, 1649
ApoI RAATTY 5 cut(s) 923, 1145, 1286, 1634, 1871
AseI ATTAAT 1 cut(s) 63
Asp700I GAANNNNTTC 1 cut(s) 1872
Asp718I GGTACC 1 cut(s) 1459
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 7 cut(s) 184, 326, 1112, 1337, 1612, 1792, 1880
AsuC2I CCSGG 2 cut(s) 980, 1495
AsuHPI GGTGA 3 cut(s) 563, 1225, 1282
AsuII TTCGAA 1 cut(s) 1047
AvaI CYCGRG 1 cut(s) 250
AvaII GGWCC 5 cut(s) 326, 1112, 1337, 1612, 1792
BaeGI GKGCMC 3 cut(s) 387, 705, 879
BalI TGGCCA 1 cut(s) 1351
BanI GGYRCC 2 cut(s) 523, 1459
BanII GRGCYC 1 cut(s) 1101
BarI GAAGNNNNNNTAC 2 cut(s) 1676, 1708
BbsI GAAGAC 2 cut(s) 264, 685
Bbv12I GWGCWC 1 cut(s) 705
BbvI GCAGC 9 cut(s) 158, 262, 265, 284, 293, 979, 1244, 1507, 1661
BceAI ACGGC 1 cut(s) 1762
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 3 cut(s) 26, 74, 1353
BciVI GTATCC 1 cut(s) 614
BcnI CCSGG 2 cut(s) 980, 1495
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 5 cut(s) 225, 687, 962, 1032, 1470
BfmI CTRYAG 1 cut(s) 279
BfrI CTTAAG 1 cut(s) 893
BfuI GTATCC 1 cut(s) 614
BisI GCNGC 9 cut(s) 172, 273, 276, 279, 282, 993, 1233, 1521, 1650
BlsI GCNGC 9 cut(s) 173, 274, 277, 280, 283, 994, 1234, 1522, 1651
BmcAI AGTACT 1 cut(s) 1141
Bme1390I CCNGG 5 cut(s) 26, 74, 980, 1353, 1495
Bme18I GGWCC 5 cut(s) 326, 1112, 1337, 1612, 1792
BmeT110I CYCGRG 1 cut(s) 250
BmgT120I GGNCC 7 cut(s) 184, 326, 1112, 1337, 1612, 1792, 1880
BmiI GGNNCC 6 cut(s) 525, 1063, 1338, 1371, 1461, 1793
BmrFI CCNGG 5 cut(s) 26, 74, 980, 1353, 1495
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 9 cut(s) 183, 259, 330, 568, 669, 943, 979, 1507, 1900
BmuI ACTGGG 1 cut(s) 104
BpiI GAAGAC 2 cut(s) 264, 685
BpmI CTGGAG 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 1047
BpuEI CTTGAG 2 cut(s) 256, 456
BpuMI CCSGG 2 cut(s) 980, 1495
Bsa29I ATCGAT 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 1509
BsaJI CCNNGG 7 cut(s) 72, 738, 1008, 1131, 1365, 1374, 1929
BsaXI ACNNNNNCTCC 2 cut(s) 1410, 1440
Bsc4I CCNNNNNNNGG 8 cut(s) 79, 510, 719, 886, 1162, 1265, 1380, 1397
Bse1I ACTGG 3 cut(s) 110, 636, 793
Bse3DI GCAATG 3 cut(s) 822, 1530, 1697
BseBI CCWGG 3 cut(s) 26, 74, 1353
BseCI ATCGAT 1 cut(s) 338
BseDI CCNNGG 7 cut(s) 72, 738, 1008, 1131, 1365, 1374, 1929
BseGI GGATG 3 cut(s) 515, 769, 1385
BseLI CCNNNNNNNGG 8 cut(s) 79, 510, 719, 886, 1162, 1265, 1380, 1397
BseMI GCAATG 3 cut(s) 822, 1530, 1697
BseNI ACTGG 3 cut(s) 110, 636, 793
BseSI GKGCMC 3 cut(s) 387, 705, 879
BseXI GCAGC 9 cut(s) 158, 262, 265, 284, 293, 979, 1244, 1507, 1661
BseYI CCCAGC 1 cut(s) 1160
BsgI GTGCAG 1 cut(s) 957
Bsh1236I CGCG 1 cut(s) 1643
BshFI GGCC 8 cut(s) 185, 233, 503, 575, 635, 1155, 1351, 1882
BshNI GGYRCC 2 cut(s) 523, 1459
BshVI ATCGAT 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 705
BsiHKCI CYCGRG 1 cut(s) 250
BsiSI CCGG 2 cut(s) 979, 1494
BslFI GGGAC 4 cut(s) 1042, 1350, 1805, 1915
BslI CCNNNNNNNGG 8 cut(s) 79, 510, 719, 886, 1162, 1265, 1380, 1397
BsmAI GTCTC 1 cut(s) 240
BsmFI GGGAC 4 cut(s) 1042, 1350, 1805, 1915
BsnI GGCC 8 cut(s) 185, 233, 503, 575, 635, 1155, 1351, 1882
BsoBI CYCGRG 1 cut(s) 250
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 4 cut(s) 387, 705, 879, 1101
Bsp143I GATC 6 cut(s) 361, 780, 1053, 1081, 1465, 1767
Bsp19I CCATGG 2 cut(s) 1008, 1929
BspACI CCGC 2 cut(s) 521, 1652
BspANI GGCC 8 cut(s) 185, 233, 503, 575, 635, 1155, 1351, 1882
BspDI ATCGAT 1 cut(s) 338
BspFNI CGCG 1 cut(s) 1643
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 6 cut(s) 525, 1063, 1338, 1371, 1461, 1793
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 3 cut(s) 788, 1460, 1775
BspT104I TTCGAA 1 cut(s) 1047
BspT107I GGYRCC 2 cut(s) 523, 1459
BspTI CTTAAG 1 cut(s) 893
BsrDI GCAATG 3 cut(s) 822, 1530, 1697
BsrI ACTGG 3 cut(s) 110, 636, 793
BssECI CCNNGG 7 cut(s) 72, 738, 1008, 1131, 1365, 1374, 1929
BssMI GATC 6 cut(s) 361, 780, 1053, 1081, 1465, 1767
BssNI GRCGYC 1 cut(s) 1509
BssT1I CCWWGG 5 cut(s) 738, 1008, 1131, 1374, 1929
Bst2UI CCWGG 3 cut(s) 26, 74, 1353
Bst4CI ACNGT 5 cut(s) 551, 886, 1604, 1859, 1946
Bst6I CTCTTC 1 cut(s) 223
BstACI GRCGYC 1 cut(s) 1509
BstAFI CTTAAG 1 cut(s) 893
BstAPI GCANNNNNTGC 2 cut(s) 278, 709
BstBI TTCGAA 1 cut(s) 1047
BstC8I GCNNGC 4 cut(s) 231, 315, 345, 1594
BstDEI CTNAG 1 cut(s) 322
BstDSI CCRYGG 2 cut(s) 1008, 1929
BstF5I GGATG 3 cut(s) 515, 769, 1385
BstFNI CGCG 1 cut(s) 1643
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 6 cut(s) 364, 783, 1056, 1084, 1468, 1770
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 6 cut(s) 361, 780, 1053, 1081, 1465, 1767
BstNI CCWGG 3 cut(s) 26, 74, 1353
BstNSI RCATGY 1 cut(s) 709
BstSCI CCNGG 5 cut(s) 24, 72, 978, 1351, 1493
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 3 cut(s) 387, 705, 879
BstUI CGCG 1 cut(s) 1643
BstV1I GCAGC 9 cut(s) 158, 262, 265, 284, 293, 979, 1244, 1507, 1661
BstV2I GAAGAC 2 cut(s) 264, 685
BstX2I RGATCY 1 cut(s) 1767
BstYI RGATCY 1 cut(s) 1767
Bsu15I ATCGAT 1 cut(s) 338
BsuI GTATCC 1 cut(s) 614
BsuRI GGCC 8 cut(s) 185, 233, 503, 575, 635, 1155, 1351, 1882
BsuTUI ATCGAT 1 cut(s) 338
BtgI CCRYGG 2 cut(s) 1008, 1929
BtsCI GGATG 3 cut(s) 515, 769, 1385
BtsIMutI CAGTG 4 cut(s) 117, 643, 834, 1609
Cac8I GCNNGC 4 cut(s) 231, 315, 345, 1594
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 783
CfoI GCGC 1 cut(s) 745
Cfr13I GGNCC 7 cut(s) 184, 326, 1112, 1337, 1612, 1792, 1880
ClaI ATCGAT 1 cut(s) 338
CseI GACGC 1 cut(s) 1649
CsiI ACCWGGT 1 cut(s) 24
Csp6I GTAC 4 cut(s) 153, 1140, 1460, 1584
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 8 cut(s) 79, 706, 784, 1009, 1229, 1764, 1788, 1930
CviQI GTAC 4 cut(s) 153, 1140, 1460, 1584
DdeI CTNAG 1 cut(s) 322
DpnI GATC 6 cut(s) 363, 782, 1055, 1083, 1467, 1769
DpnII GATC 6 cut(s) 361, 780, 1053, 1081, 1465, 1767
DraIII CACNNNGTG 1 cut(s) 1265
EaeI YGGCCR 2 cut(s) 573, 1349
Eam1104I CTCTTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 223
Eco130I CCWWGG 5 cut(s) 738, 1008, 1131, 1374, 1929
Eco147I AGGCCT 2 cut(s) 503, 1155
Eco24I GRGCYC 1 cut(s) 1101
Eco47I GGWCC 5 cut(s) 326, 1112, 1337, 1612, 1792
Eco57I CTGAAG 1 cut(s) 810
Eco88I CYCGRG 1 cut(s) 250
EcoO109I RGGNCCY 3 cut(s) 1337, 1792, 1880
EcoRII CCWGG 3 cut(s) 24, 72, 1351
EcoT14I CCWWGG 5 cut(s) 738, 1008, 1131, 1374, 1929
EcoT22I ATGCAT 1 cut(s) 1765
EcoT38I GRGCYC 1 cut(s) 1101
ErhI CCWWGG 5 cut(s) 738, 1008, 1131, 1374, 1929
FaeI CATG 8 cut(s) 82, 709, 787, 1012, 1232, 1767, 1791, 1933
FalI AAGNNNNNCTT 2 cut(s) 1611, 1643
FaqI GGGAC 4 cut(s) 1042, 1350, 1805, 1915
FatI CATG 8 cut(s) 78, 705, 783, 1008, 1228, 1763, 1787, 1929
FauNDI CATATG 2 cut(s) 1396, 1540
Fnu4HI GCNGC 9 cut(s) 172, 273, 276, 279, 282, 993, 1233, 1521, 1650
FokI GGATG 3 cut(s) 522, 776, 1392
FriOI GRGCYC 1 cut(s) 1101
Fsp4HI GCNGC 9 cut(s) 172, 273, 276, 279, 282, 993, 1233, 1521, 1650
FspBI CTAG 5 cut(s) 225, 687, 962, 1032, 1470
GlaI GCGC 1 cut(s) 744
GluI GCNGC 9 cut(s) 172, 273, 276, 279, 282, 993, 1233, 1521, 1650
GsaI CCCAGC 1 cut(s) 1164
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 8 cut(s) 185, 233, 503, 575, 635, 1155, 1351, 1882
HapII CCGG 2 cut(s) 979, 1494
HgaI GACGC 1 cut(s) 1649
HhaI GCGC 1 cut(s) 745
Hin1I GRCGYC 1 cut(s) 1509
Hin1II CATG 8 cut(s) 82, 709, 787, 1012, 1232, 1767, 1791, 1933
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HindIII AAGCTT 1 cut(s) 1089
HpaII CCGG 2 cut(s) 979, 1494
HphI GGTGA 3 cut(s) 563, 1225, 1282
Hpy166II GTNNAC 4 cut(s) 538, 703, 1268, 1690
Hpy188I TCNGA 3 cut(s) 193, 432, 449
Hpy8I GTNNAC 4 cut(s) 538, 703, 1268, 1690
Hpy99I CGWCG 2 cut(s) 1511, 1643
HpyAV CCTTC 5 cut(s) 423, 761, 842, 1206, 1930
HpyCH4III ACNGT 5 cut(s) 551, 886, 1604, 1859, 1946
HpyCH4IV ACGT 2 cut(s) 261, 1509
HpyF3I CTNAG 1 cut(s) 322
HpySE526I ACGT 2 cut(s) 261, 1509
Hsp92I GRCGYC 1 cut(s) 1509
Hsp92II CATG 8 cut(s) 82, 709, 787, 1012, 1232, 1767, 1791, 1933
HspAI GCGC 1 cut(s) 743
KpnI GGTACC 1 cut(s) 1463
Kzo9I GATC 6 cut(s) 361, 780, 1053, 1081, 1465, 1767
LmnI GCTCC 1 cut(s) 889
Lsp1109I GCAGC 9 cut(s) 158, 262, 265, 284, 293, 979, 1244, 1507, 1661
LweI GCATC 9 cut(s) 183, 259, 330, 568, 669, 943, 979, 1507, 1900
MabI ACCWGGT 1 cut(s) 24
MaeI CTAG 5 cut(s) 225, 687, 962, 1032, 1470
MaeII ACGT 2 cut(s) 261, 1509
MaeIII GTNAC 1 cut(s) 90
MalI GATC 6 cut(s) 363, 782, 1055, 1083, 1467, 1769
MboI GATC 6 cut(s) 361, 780, 1053, 1081, 1465, 1767
MboII GAAGA 8 cut(s) 210, 269, 296, 685, 816, 1762, 1816, 1880
MfeI CAATTG 1 cut(s) 1242
MflI RGATCY 1 cut(s) 1767
MhlI GDGCHC 4 cut(s) 387, 705, 879, 1101
MlsI TGGCCA 1 cut(s) 1351
MluI ACGCGT 1 cut(s) 1641
MluNI TGGCCA 1 cut(s) 1351
MlyI GAGTC 3 cut(s) 242, 442, 699
MmeI TCCRAC 1 cut(s) 216
Mox20I TGGCCA 1 cut(s) 1351
Mph1103I ATGCAT 1 cut(s) 1765
MroXI GAANNNNTTC 1 cut(s) 1872
MscI TGGCCA 1 cut(s) 1351
MseI TTAA 5 cut(s) 56, 63, 671, 894, 1863
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 1351
MspA1I CMGCKG 2 cut(s) 275, 1652
MspCI CTTAAG 1 cut(s) 893
MspI CCGG 2 cut(s) 979, 1494
MspR9I CCNGG 5 cut(s) 26, 74, 980, 1353, 1495
MunI CAATTG 1 cut(s) 1242
MvaI CCWGG 3 cut(s) 26, 74, 1353
MvnI CGCG 1 cut(s) 1643
NciI CCSGG 2 cut(s) 980, 1495
NcoI CCATGG 2 cut(s) 1008, 1929
NdeI CATATG 2 cut(s) 1396, 1540
NdeII GATC 6 cut(s) 361, 780, 1053, 1081, 1465, 1767
NlaIII CATG 8 cut(s) 82, 709, 787, 1012, 1232, 1767, 1791, 1933
NlaIV GGNNCC 6 cut(s) 525, 1063, 1338, 1371, 1461, 1793
NmuCI GTSAC 1 cut(s) 90
NsiI ATGCAT 1 cut(s) 1765
NspI RCATGY 1 cut(s) 709
NspV TTCGAA 1 cut(s) 1047
PaeR7I CTCGAG 1 cut(s) 250
PagI TCATGA 1 cut(s) 783
PceI AGGCCT 2 cut(s) 503, 1155
PciI ACATGT 1 cut(s) 705
PdmI GAANNNNTTC 1 cut(s) 1872
PfeI GAWTC 7 cut(s) 143, 301, 464, 1330, 1358, 1561, 1712
PflFI GACNNNGTC 1 cut(s) 263
PflMI CCANNNNNTGG 3 cut(s) 886, 1265, 1380
PkrI GCNGC 9 cut(s) 173, 274, 277, 280, 283, 994, 1234, 1522, 1651
PleI GAGTC 3 cut(s) 242, 441, 698
PpsI GAGTC 3 cut(s) 242, 441, 698
PpuMI RGGWCCY 2 cut(s) 1337, 1792
PscI ACATGT 1 cut(s) 705
PshBI ATTAAT 1 cut(s) 63
Psp5II RGGWCCY 2 cut(s) 1337, 1792
Psp6I CCWGG 3 cut(s) 24, 72, 1351
PspFI CCCAGC 1 cut(s) 1160
PspGI CCWGG 3 cut(s) 24, 72, 1351
PspN4I GGNNCC 6 cut(s) 525, 1063, 1338, 1371, 1461, 1793
PspPI GGNCC 7 cut(s) 184, 326, 1112, 1337, 1612, 1792, 1880
PspPPI RGGWCCY 2 cut(s) 1337, 1792
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 1767
PsyI GACNNNGTC 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 4 cut(s) 154, 1141, 1461, 1585
RsaNI GTAC 4 cut(s) 153, 1140, 1460, 1584
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 5 cut(s) 56, 63, 671, 894, 1863
SatI GCNGC 9 cut(s) 172, 273, 276, 279, 282, 993, 1233, 1521, 1650
Sau3AI GATC 6 cut(s) 361, 780, 1053, 1081, 1465, 1767
Sau96I GGNCC 7 cut(s) 184, 326, 1112, 1337, 1612, 1792, 1880
ScaI AGTACT 1 cut(s) 1141
SchI GAGTC 3 cut(s) 242, 442, 699
ScrFI CCNGG 5 cut(s) 26, 74, 980, 1353, 1495
SduI GDGCHC 4 cut(s) 387, 705, 879, 1101
SexAI ACCWGGT 1 cut(s) 24
SfaNI GCATC 9 cut(s) 183, 259, 330, 568, 669, 943, 979, 1507, 1900
SfcI CTRYAG 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 250
SfuI TTCGAA 1 cut(s) 1047
SinI GGWCC 5 cut(s) 326, 1112, 1337, 1612, 1792
SlaI CTCGAG 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 4 cut(s) 235, 250, 471, 893
SmoI CTYRAG 4 cut(s) 235, 250, 471, 893
SseBI AGGCCT 2 cut(s) 503, 1155
SsiI CCGC 2 cut(s) 521, 1652
SspMI CTAG 5 cut(s) 225, 687, 962, 1032, 1470
StuI AGGCCT 2 cut(s) 503, 1155
StyD4I CCNGG 5 cut(s) 24, 72, 978, 1351, 1493
StyI CCWWGG 5 cut(s) 738, 1008, 1131, 1374, 1929
TaaI ACNGT 5 cut(s) 551, 886, 1604, 1859, 1946
TaiI ACGT 2 cut(s) 264, 1512
TaqII GACCGA 1 cut(s) 343
TatI WGTACW 2 cut(s) 152, 1139
TfiI GAWTC 7 cut(s) 143, 301, 464, 1330, 1358, 1561, 1712
Tru1I TTAA 5 cut(s) 56, 63, 671, 894, 1863
Tru9I TTAA 5 cut(s) 56, 63, 671, 894, 1863
TscAI CASTG 4 cut(s) 117, 643, 834, 1609
TseFI GTSAC 1 cut(s) 90
TseI GCWGC 9 cut(s) 171, 272, 275, 278, 281, 992, 1232, 1520, 1649
Tsp45I GTSAC 1 cut(s) 90
TspDTI ATGAA 7 cut(s) 17, 135, 685, 780, 1754, 1816, 1956
TspGWI ACGGA 1 cut(s) 1724
TspRI CASTG 4 cut(s) 117, 643, 834, 1609
Tth111I GACNNNGTC 1 cut(s) 263
Van91I CCANNNNNTGG 3 cut(s) 886, 1265, 1380
Vha464I CTTAAG 1 cut(s) 893
VneI GTGCAC 1 cut(s) 701
VpaK11BI GGWCC 5 cut(s) 326, 1112, 1337, 1612, 1792
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 5 cut(s) 923, 1145, 1286, 1634, 1871
XceI RCATGY 1 cut(s) 709
XcmI CCANNNNNNNNNTGG 2 cut(s) 1207, 1248
XhoI CTCGAG 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 1872
XspI CTAG 5 cut(s) 225, 687, 962, 1032, 1470
ZraI GACGTC 1 cut(s) 1510
ZrmI AGTACT 1 cut(s) 1141
Zsp2I ATGCAT 1 cut(s) 1765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.