MD08G1049000.v1.1

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000051
Physical Location & Seq
Reverse (-)
3829418 .. 3832823
3406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1049000.v1.1.491

Sequence Viewer

Length: 2874 bp
ATGAATTCGTTGGCTATGGGACACAAGTCCCCGAACATAACAATCGCAGTGGAAGTTGGTGTGATTCTTGATGACCTTGATTCACTCAGTGCAAAAGTTTGGTTGAGCTGCATTAACATGGCCCTCTCAGATTTCTATACCGGCTCTCATGCTTCCTCTTCTACCAGGGTGGTTCTAAACGTTAGGGACTCCGAAGAAGACACTGTCGATGCAGCTGCTGCAGCATTAGACCTGATAAAGAATGCTCAAGTGCAGGCAATCCTCGGCCCAAAGTCATCGATGCAAGCCAAATTTGTAATTGATCTGGGGAACAAAGCCCAGGTGCCCATCATCTCATTTTCAGCAACAGATCCTTCCCTCACTTCTATTCGAAGTCCATACTTTTACCGAGCCGCACAAAATGACTCATCTCAGGTGAAAGCCATAAGTGCTATCATCCAAGCCTTTGGATGGAGAGAAGCTGTGCCCATCTATGTAGACAACGAGTATGGCGAGGGAATAATACCTTACTTGGTAGATGCCTTGCAAGAAGTTGAAGCTCGTGTCCCATATCGAAGTGTTATACCTCCAGAAGCCACTGAGGACCAGCTTGATGCAGAGCTCTCCAAGCTAATGACTATGCAGACTAGAGTCTTCATTGTGCACATGTTGCCCTCTCTTGGCTCTCGCCTTTTTGCCAAGGCACGAGAGAAGGGAATGATGGATGAAGGGTATGTTTGGATAATGACCAATGGGTTAACCAATCTTTTGAGTACAACAAATGCTTCAGTCATCAATTCAATGCAAGGTGCACTGGGTGTGAGGACTTATGTGCCCAGAACAGAAGAATATATCAATTTTAGAAATCGATGGAAAAGGCAATTCCAAAAGAAGAATCCAACAATTATCGACGCTGAATTAGACGTTCCTTCACTCTGGGCTTATGATGCTGCTTTTGCATTAGCCATGGCAGTTGAAAATGTTGGGACTACAAGCTTTGTCTTCAAAAAGGCAAATGCTTCCACCAACTCATCAAATGATCTTGGATCAATTGGGATCTCCCAAAATGGTCCAGAGCTTTGCAAATCCTTGTCAGGTACTAAATTTAGAGGCCTTAGTGGGGATTTCAGTTTTGTTGATGGGCAATTACAAGCATCCGTCTTTGAAATGGTAAATGTGTTGGGTGATGCAACAAAAACAATTGGGTTTTGGACACCACAGAGTGGACTTGTGAAAAAATTGAACTTGATAAACACAAGTGGTCCTTATTCAACTTCTAAATCCAATGTTGGACCTATTATATGGCCCGGAGATTCTACCTCAGTTCCTAAGGGATGGGAGATCCCGACAAACGGCAAAAGATTGAGAGTAGGAGTTCCTGTGGAGAATGGCTATCCTCAATTTGTAGAAGTAGTATATGACTCTAGCACTAATACGATTCATGTTGCTGGGTACGTCATTGACATCTTCAACGCTGCAATGGGCAAATTAAAGTACAATGTTGCTTATGATTTCATTCCCTTCGCCAATCCAGATGGTACGAGTGCTGGCACTTACGATGATTTGGTGCATCAAGTATTTCTCGGGAATTTTGATGCAGTGGCAGCAGATGCAACAATTAGAGCAAACAGGTCCTTGTATGTGGACTTTACCTTGCCATACACAGAATCAGGGGTAGTGATGGTAGTGCCAACGAAAGATAGAAAGAGCAAAAGTGCATGGATTTTCTTAAAGCCTTTGACACTGAATCTTTGGTTGACAAGTGCTTTCTTTTTCGTCTTCGTTGGTTCAGTGGTTTGGGTTCTTGAACACCGTATCAATGATGAATTTCGCGGTCCTCCTGCTCATCAAGTTGGCACAGTCTTTTGGTTCTCCTTCTCAACCATGGTTTTTGCACAGCGAGAGAGGGTGATTGGCAACTTGGCAAGGTCTGTGGAGATTGTTTGGATGTTTGTAGTGCTGATACTAACGCAAAGTTACACAGCGAGTCTAACTTCATTGTTAACATCTCAAGAACTTCAGCCAACTTTTACGAATATATTCGATCTTATAAACAATAAGGAGTATATTGGGTACAAAAACGGTTCTTTCGTCCGCGACCTTCTGATAGACCGGGGTGTTGATCCCTCCAAGTTACGGCCTTACACGTCTAGGGAAGAATGTGACGTATTTTTGACAAATGGGAGTGCCAAAGGCGGTATTGCTGCTGCTATCGACGAAACCCCCAACATTAGGCTGTTTCTTGCCAAATATTGCAAGAAATACACCATGATTGGCCCAATTTTCAGAACTGATGGCTTTGGCTTTGTCTTCCCCAAAGGTTCACCTCTTGTGCCAGATGTTTCAAGGGCAATCCTGGTCGTGAATGAGGGAAATGATACAACTGATGCTGAAAACAAATGGTTCGCGCCGGAAGGCACATGTTCAGATACATCACCCACCATCTCCGATTCCAACAGTCTTGGACTTGATAGCTTTTGGAGTCTCTTCCTCATTGTCGGGGTCTCCGCATCTTTAGCTCTTCTCATTTTTGCAGTCTCTTTGTTTAATAGGCATTGGCATGTCTTCATCGCGACAAGAGGTGACTCTGTGTGGAGAAGATTTATGGTTATGCTTAGAACCTTTGACCAAAGAGACCTTAGCTCCCATACTTTTAGAAAGAGTAGTGGCTCCCGAGATGGAGAAACCTACAATTTTGGAGCTACGGCTGTCGAGGATTCACCAAACAGTTACAGCACCTGCCCACCAAGCCCTGCCTCAGGCCATTCAGACCACCCAGAAATACTGATTACAGCTGAAGGAGCTCACCCACGCATGGGTGAACAGAACAGGTCCAAGGCCCAAACTTCCGACACAGTTCCGCAACAGCCAGGAGAAATCCTTCTAACGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

958

Amino Acids

105.23

Weight (kDa)

5.62

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 28 - 325 2.7e-20 Periplasmic binding protein
ANF_receptor PF01094 35 - 386 1.4e-85 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 466 - 557 6.7e-07 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 471 - 796 1.1e-07 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 575 - 829 6.2e-36 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24710 AT2G24710 AT2G24710 AT2G24720 AT2G29100 AT2G29100 AT2G29110 AT2G29110 AT2G29120 AT5G27100
fragaria_vesca FvH4_2g11260 FvH4_2g11280 FvH4_2g11310 FvH4_2g11310 FvH4_2g11331 FvH4_2g11331 FvH4_2g11340 FvH4_2g11340 FvH4_2g11340 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31990 FvH4_2g37140 FvH4_2g37150 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37161 FvH4_2g37161 FvH4_2g37163 FvH4_2g37163 FvH4_2g37170 FvH4_2g37181 FvH4_2g37191 FvH4_3g22781 FvH4_3g22781 FvH4_3g22781 FvH4_3g22790 FvH4_3g22790 FvH4_3g22810 FvH4_5g13460 FvH4_5g34751 FvH4_7g09130 FvH4_7g27991 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010
malus_domestica MD08G1047600.v1.1 MD08G1047700.v1.1 MD08G1048100.v1.1 MD08G1048200.v1.1 MD08G1048300.v1.1 MD08G1048500.v1.1 MD08G1048600.v1.1 MD08G1048700.v1.1 MD08G1048900.v1.1 MD08G1049000.v1.1 MD14G1064700.v1.1 MD15G1045900.v1.1 MD15G1046000.v1.1 MD15G1046200.v1.1 MD15G1046400.v1.1 MD15G1059200.v1.1 MD15G1059500.v1.1 MD15G1059600.v1.1 MD15G1059700.v1.1
prunus_persica Prupe.1G399500_v2.0.a1 Prupe.1G399600_v2.0.a1 Prupe.1G399700_v2.0.a1 Prupe.1G399800_v2.0.a1 Prupe.1G399900_v2.0.a1 Prupe.1G400000_v2.0.a1 Prupe.1G400300_v2.0.a1 Prupe.1G400400_v2.0.a1 Prupe.1G400500_v2.0.a1 Prupe.1G400600_v2.0.a1 Prupe.1G400700_v2.0.a1 Prupe.1G412700_v2.0.a1 Prupe.1G412800_v2.0.a1
pyrus_communis pycom02g01350 pycom08g04020 pycom08g04040 pycom08g04080 pycom08g04090 pycom08g04100 pycom14g05240 pycom15g04300 pycom15g04310 pycom15g04330 pycom15g04340 pycom15g04380 pycom15g05560 pycom15g05570 pycom15g05580 pycom15g05600
rosa_chinensis RchiOBHm_Chr1g0345071 RchiOBHm_Chr1g0345081 RchiOBHm_Chr1g0345171 RchiOBHm_Chr3g0480811 RchiOBHm_Chr3g0492581 RchiOBHm_Chr5g0020871 RchiOBHm_Chr5g0039801 RchiOBHm_Chr5g0039811 RchiOBHm_Chr5g0039861 RchiOBHm_Chr5g0039911 RchiOBHm_Chr5g0039941 RchiOBHm_Chr5g0039951 RchiOBHm_Chr5g0040011 RchiOBHm_Chr6g0269601 RchiOBHm_Chr6g0269621 RchiOBHm_Chr6g0269631 RchiOBHm_Chr6g0269641 RchiOBHm_Chr6g0269671 RchiOBHm_Chr6g0269681 RchiOBHm_Chr6g0269731 RchiOBHm_Chr6g0269751 RchiOBHm_Chr6g0269761 RchiOBHm_Chr6g0269771 RchiOBHm_Chr6g0269781 RchiOBHm_Chr6g0269861 RchiOBHm_Chr6g0269871 RchiOBHm_Chr6g0269881 RchiOBHm_Chr6g0269911 RchiOBHm_Chr6g0269921 RchiOBHm_Chr6g0269941 RchiOBHm_Chr6g0269951 RchiOBHm_Chr6g0304511 RchiOBHm_Chr6g0304621 RchiOBHm_Chr6g0304641 RchiOBHm_Chr6g0304651 RchiOBHm_Chr6g0304661 RchiOBHm_Chr6g0310781 RchiOBHm_Chr6g0310801 RchiOBHm_Chr6g0310811 RchiOBHm_Chr6g0310821 RchiOBHm_Chr6g0310831 RchiOBHm_Chr7g0183621 RchiOBHm_Chr7g0183651
rosa_laevigata RLG00000005035 RLG00000005036 RLG00000010458 RLG00000010459 RLG00000010460 RLG00000010961 RLG00000010962 RLG00000010963 RLG00000010964 RLG00000010966 RLG00000010970 RLG00000013824 RLG00000013825 RLG00000013826 RLG00000013828 RLG00000013829 RLG00000013831 RLG00000013833 RLG00000013835 RLG00000013837 RLG00000013840 RLG00000013842 RLG00000013844 RLG00000022758 RLG00000023449 RLG00000026705 RLG00000028849 RLG00000028853 RLG00000028854 RLG00000032569 RLG00000033944 RLG00000033945 RLG00000033947 RLG00000033949 RLG00000033951 RLG00000034239
rosa_multiflora Rmu_co8287891.1_g000001 Rmu_co8428745.1_g000001 Rmu_co8521767.1_g000002 Rmu_sc0000103.1_g000022 Rmu_sc0000103.1_g000031 Rmu_sc0000103.1_g000041 Rmu_sc0000103.1_g000053 Rmu_sc0000247.1_g000028 Rmu_sc0000247.1_g000051 Rmu_sc0000893.1_g000003 Rmu_sc0000893.1_g000017 Rmu_sc0001143.1_g000008 Rmu_sc0001143.1_g000019 Rmu_sc0001143.1_g000023 Rmu_sc0001975.1_g000003 Rmu_sc0001975.1_g000004 Rmu_sc0001975.1_g000005 Rmu_sc0001975.1_g000012 Rmu_sc0002151.1_g000006 Rmu_sc0003610.1_g000021 Rmu_sc0003682.1_g000001 Rmu_sc0003863.1_g000004 Rmu_sc0003863.1_g000007 Rmu_sc0003863.1_g000013 Rmu_sc0004196.1_g000005 Rmu_sc0004515.1_g000013 Rmu_sc0004515.1_g000024 Rmu_sc0004688.1_g000001 Rmu_sc0004688.1_g000003 Rmu_sc0004688.1_g000004 Rmu_sc0004748.1_g000006 Rmu_sc0004838.1_g000016 Rmu_sc0004838.1_g000017 Rmu_sc0005532.1_g000006 Rmu_sc0005532.1_g000011 Rmu_sc0005950.1_g000004 Rmu_sc0006576.1_g000005 Rmu_sc0006576.1_g000021 Rmu_sc0008520.1_g000002 Rmu_sc0008520.1_g000004 Rmu_sc0009130.1_g000002 Rmu_sc0018719.1_g000001 Rmu_sc0027713.1_g000005 Rmu_sc0040804.1_g000001 Rmu_ssc0000218.1_g000059 Rmu_ssc0000218.1_g000062 Rmu_ssc0000218.1_g000068 Rmu_ssc0000395.1_g000008 Rmu_ssc0000395.1_g000009 Rmu_ssc0000395.1_g000010 Rmu_ssc0000395.1_g000011 Rmu_ssc0000395.1_g000013 Rmu_ssc0000395.1_g000014
rosa_roxburghii Rroxscaffold_1G00041270 Rroxscaffold_1G00041290 Rroxscaffold_1G00041300 Rroxscaffold_1G00041360 Rroxscaffold_1G00041380 Rroxscaffold_1G00057500 Rroxscaffold_3G00270350 Rroxscaffold_4G00283160 Rroxscaffold_4G00309210 Rroxscaffold_4G00309240 Rroxscaffold_4G00309260 Rroxscaffold_4G00309290 Rroxscaffold_4G00309360 Rroxscaffold_6G00392210 Rroxscaffold_6G00401030 Rroxscaffold_7G00158260 Rroxscaffold_7G00163620 Rroxscaffold_7G00163630 Rroxscaffold_7G00163650 Rroxscaffold_7G00163680 Rroxscaffold_7G00198270 Rroxscaffold_7G00198280 Rroxscaffold_7G00198330 Rroxscaffold_7G00198340 Rroxscaffold_7G00198360 Rroxscaffold_7G00198390 Rroxscaffold_7G00198410 Rroxscaffold_7G00198450 Rroxscaffold_7G00198460 Rroxscaffold_7G00198490 Rroxscaffold_7G00198500 Rroxscaffold_7G00198510 Rroxscaffold_7G00198530 Rroxscaffold_7G00198550 Rroxscaffold_7G00198600
rosa_rugosa Rorug01G0175900 Rorug01G0176200 Rorug03G0186700 Rorug03G0254500 Rorug05G0182700 Rorug05G0183000 Rorug05G0183000 Rorug06G0049000 Rorug06G0049500 Rorug06G0049600.1 Rorug06G0049700 Rorug06G0050000 Rorug06G0050000 Rorug06G0050100 Rorug06G0050300.1 Rorug06G0050400 Rorug06G0051100 Rorug06G0051200 Rorug06G0051300 Rorug06G0051500 Rorug06G0051600 Rorug06G0051700 Rorug06G0332600 Rorug06G0332700 Rorug06G0332700 Rorug06G0383100 Rorug06G0383200 Rorug06G0383300 Rorug06G0383400 Rorug06G0459100 Rorug06G0459200
rosa_samantha Rh1BG160400 Rh1BG160500 Rh1BG160700 Rh3DG263900 Rh3DG338400 Rh5CG164400 Rh5CG164500 Rh5CG305500 Rh5CG305900 Rh5CG306000 Rh5DG280800 Rh5DG281300 Rh6AG445300 Rh6AG497000 Rh6BG174700 Rh6BG457200 Rh6CG168900 Rh6CG169800 Rh6CG512700 Rh6CG512900 Rh6DG034100 Rh6DG160500 Rh6DG160800 Rh6DG160900 Rh6DG161100 Rh6DG161400 Rh6DG161800 Rh6DG162000 Rh6DG162100 Rh6DG162200 Rh6DG444800 Rh6DG445000 Rh6DG445100 Rh6DG445200 Rh6DG445300 Rh6DG499100 Rh6DG499200 Rh6DG499300 Rh6DG499400 Rh6DG499500 Rh6DG499700 Rh7BG059800 Rh7BG060000 Rh7BG258500 Rh7BG258700
rosa_wichuraiana Rw0G006180 Rw0G007950 Rw0G018490 Rw0G022090 Rw1G015970 Rw1G015990 Rw3G021330 Rw5G025120 Rw5G025140 Rw5G025150 Rw5G025160 Rw5G025180 Rw5G025830 Rw5G025850 Rw6G014550 Rw6G014570 Rw6G014580 Rw6G014600 Rw6G014620 Rw6G014650 Rw6G014670 Rw6G038650 Rw6G038670 Rw6G038680 Rw6G038700 Rw6G038710 Rw6G038720 Rw6G043280 Rw6G043290 Rw7G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2030
AarI CACCTGC 1 cut(s) 2726
Acc36I ACCTGC 1 cut(s) 2726
AccB1I GGYRCC 1 cut(s) 322
AccB7I CCANNNNNTGG 1 cut(s) 1202
AccI GTMKAC 1 cut(s) 477
AccII CGCG 4 cut(s) 1812, 2076, 2387, 2552
AciI CCGC 6 cut(s) 393, 1812, 2074, 2175, 2487, 2840
AclI AACGTT 1 cut(s) 180
AclWI GGATC 5 cut(s) 344, 1033, 1043, 1315, 2096
AcsI RAATTY 5 cut(s) 4, 290, 1082, 1567, 1805
AcuI CTGAAG 3 cut(s) 750, 1982, 2796
AdeI CACNNNGTG 3 cut(s) 89, 797, 1202
AfaI GTAC 6 cut(s) 754, 1078, 1433, 1475, 1519, 2054
AflIII ACRYGT 3 cut(s) 645, 2124, 2399
AjiI CACGTC 1 cut(s) 2127
AjnI CCWGG 4 cut(s) 164, 318, 2334, 2848
Alw21I GWGCWC 4 cut(s) 603, 645, 793, 2785
Alw26I GTCTC 4 cut(s) 2468, 2488, 2521, 2607
Alw44I GTGCAC 2 cut(s) 641, 789
AlwI GGATC 5 cut(s) 344, 1033, 1043, 1315, 2096
AlwNI CAGNNNCTG 2 cut(s) 218, 2718
Ama87I CYCGRG 2 cut(s) 1562, 2652
AoxI GGCC 8 cut(s) 120, 265, 1090, 1283, 2117, 2254, 2740, 2817
ApaLI GTGCAC 2 cut(s) 641, 789
ApoI RAATTY 5 cut(s) 4, 290, 1082, 1567, 1805
Asp700I GAANNNNTTC 2 cut(s) 2018, 2859
AspLEI GCGC 1 cut(s) 2389
AsuC2I CCSGG 2 cut(s) 1287, 2093
AsuHPI GGTGA 9 cut(s) 427, 1175, 1900, 2295, 2406, 2573, 2691, 2777, 2810
AsuII TTCGAA 1 cut(s) 370
AvaI CYCGRG 2 cut(s) 1562, 2652
AvaII GGWCC 7 cut(s) 583, 1049, 1241, 1271, 1611, 1814, 2811
AxyI CCTNAGG 2 cut(s) 1308, 2737
BaeGI GKGCMC 5 cut(s) 327, 468, 645, 793, 816
BanI GGYRCC 1 cut(s) 322
BanII GRGCYC 2 cut(s) 603, 2785
BauI CACGAG 2 cut(s) 540, 684
BbsI GAAGAC 6 cut(s) 204, 625, 973, 1750, 2281, 2536
Bbv12I GWGCWC 4 cut(s) 603, 645, 793, 2785
BceAI ACGGC 4 cut(s) 1348, 2132, 2700, 2854
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 4 cut(s) 166, 320, 2336, 2850
BcnI CCSGG 2 cut(s) 1287, 2093
BcoDI GTCTC 4 cut(s) 2468, 2488, 2521, 2607
BfaI CTAG 3 cut(s) 627, 1404, 2130
BfmI CTRYAG 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 2726
Bme1390I CCNGG 6 cut(s) 166, 320, 1287, 2093, 2336, 2850
Bme18I GGWCC 7 cut(s) 583, 1049, 1241, 1271, 1611, 1814, 2811
BmeT110I CYCGRG 2 cut(s) 1562, 2652
BmgBI CACGTC 1 cut(s) 2127
BmiI GGNNCC 2 cut(s) 324, 2650
BmrFI CCNGG 6 cut(s) 166, 320, 1287, 2093, 2336, 2850
BmrI ACTGGG 1 cut(s) 803
BmuI ACTGGG 1 cut(s) 803
BoxI GACNNNNGTC 2 cut(s) 25, 629
BpiI GAAGAC 6 cut(s) 204, 625, 973, 1750, 2281, 2536
BpmI CTGGAG 1 cut(s) 552
Bpu10I CCTNAGC 1 cut(s) 2618
Bpu14I TTCGAA 1 cut(s) 370
BpuEI CTTGAG 2 cut(s) 231, 1974
BpuMI CCSGG 2 cut(s) 1287, 2093
Bsa29I ATCGAT 2 cut(s) 278, 847
BsaI GGTCTC 2 cut(s) 2488, 2607
BsaJI CCNNGG 8 cut(s) 165, 262, 318, 678, 945, 1863, 2092, 2814
BsaXI ACNNNNNCTCC 4 cut(s) 1344, 1374, 2606, 2636
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 798
Bse21I CCTNAGG 2 cut(s) 1308, 2737
Bse3DI GCAATG 1 cut(s) 1464
BseBI CCWGG 4 cut(s) 166, 320, 2336, 2850
BseCI ATCGAT 2 cut(s) 278, 847
BseDI CCNNGG 8 cut(s) 165, 262, 318, 678, 945, 1863, 2092, 2814
BseGI GGATG 6 cut(s) 435, 455, 709, 1133, 1319, 1932
BseMI GCAATG 1 cut(s) 1464
BseMII CTCAG 6 cut(s) 100, 141, 425, 570, 1314, 2751
BseNI ACTGG 1 cut(s) 798
BseSI GKGCMC 5 cut(s) 327, 468, 645, 793, 816
BseYI CCCAGC 1 cut(s) 1427
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 4 cut(s) 1812, 2076, 2387, 2552
BshFI GGCC 8 cut(s) 122, 267, 1092, 1285, 2119, 2256, 2742, 2819
BshNI GGYRCC 1 cut(s) 322
BshVI ATCGAT 2 cut(s) 278, 847
BsiHKAI GWGCWC 4 cut(s) 603, 645, 793, 2785
BsiHKCI CYCGRG 2 cut(s) 1562, 2652
BsiSI CCGG 4 cut(s) 141, 1287, 2092, 2390
BslFI GGGAC 5 cut(s) 13, 33, 200, 530, 979
BsmAI GTCTC 4 cut(s) 2468, 2488, 2521, 2607
BsmFI GGGAC 5 cut(s) 13, 33, 200, 530, 979
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 8 cut(s) 122, 267, 1092, 1285, 2119, 2256, 2742, 2819
Bso31I GGTCTC 2 cut(s) 2488, 2607
BsoBI CYCGRG 2 cut(s) 1562, 2652
Bsp119I TTCGAA 1 cut(s) 370
Bsp1286I GDGCHC 7 cut(s) 327, 468, 603, 645, 793, 816, 2785
Bsp143I GATC 8 cut(s) 301, 349, 1018, 1025, 1035, 1320, 2023, 2101
Bsp19I CCATGG 2 cut(s) 945, 1863
Bsp68I TCGCGA 1 cut(s) 2552
BspACI CCGC 6 cut(s) 393, 1812, 2074, 2175, 2487, 2840
BspANI GGCC 8 cut(s) 122, 267, 1092, 1285, 2119, 2256, 2742, 2819
BspCNI CTCAG 6 cut(s) 99, 140, 424, 571, 1313, 2750
BspDI ATCGAT 2 cut(s) 278, 847
BspFNI CGCG 4 cut(s) 1812, 2076, 2387, 2552
BspLI GGNNCC 2 cut(s) 324, 2650
BspMAI CTGCAG 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 2726
BspPI GGATC 5 cut(s) 344, 1033, 1043, 1315, 2096
BspQI GCTCTTC 1 cut(s) 2505
BspT104I TTCGAA 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 322
BspTNI GGTCTC 2 cut(s) 2488, 2607
BsrDI GCAATG 1 cut(s) 1464
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 1 cut(s) 798
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 8 cut(s) 165, 262, 318, 678, 945, 1863, 2092, 2814
BssMI GATC 8 cut(s) 301, 349, 1018, 1025, 1035, 1320, 2023, 2101
BssSI CACGAG 2 cut(s) 540, 684
BssT1I CCWWGG 4 cut(s) 678, 945, 1863, 2814
Bst2BI CACGAG 2 cut(s) 540, 684
Bst2UI CCWGG 4 cut(s) 166, 320, 2336, 2850
Bst4CI ACNGT 7 cut(s) 205, 1793, 1840, 2063, 2438, 2708, 2836
Bst6I CTCTTC 3 cut(s) 163, 2471, 2505
BstAPI GCANNNNNTGC 3 cut(s) 218, 649, 1589
BstBI TTCGAA 1 cut(s) 370
BstC8I GCNNGC 3 cut(s) 255, 285, 1528
BstDSI CCRYGG 2 cut(s) 945, 1863
BstENI CCTNNNNNAGG 1 cut(s) 2736
BstF5I GGATG 6 cut(s) 435, 455, 709, 1133, 1319, 1932
BstFNI CGCG 4 cut(s) 1812, 2076, 2387, 2552
BstHHI GCGC 1 cut(s) 2389
BstKTI GATC 8 cut(s) 304, 352, 1021, 1028, 1038, 1323, 2026, 2104
BstMAI GTCTC 4 cut(s) 2468, 2488, 2521, 2607
BstMBI GATC 8 cut(s) 301, 349, 1018, 1025, 1035, 1320, 2023, 2101
BstNI CCWGG 4 cut(s) 166, 320, 2336, 2850
BstNSI RCATGY 3 cut(s) 649, 2403, 2543
BstPAI GACNNNNGTC 2 cut(s) 25, 629
BstSCI CCNGG 6 cut(s) 164, 318, 1285, 2091, 2334, 2848
BstSFI CTRYAG 1 cut(s) 219
BstSLI GKGCMC 5 cut(s) 327, 468, 645, 793, 816
BstUI CGCG 4 cut(s) 1812, 2076, 2387, 2552
BstV2I GAAGAC 6 cut(s) 204, 625, 973, 1750, 2281, 2536
BstX2I RGATCY 3 cut(s) 349, 1035, 1320
BstXI CCANNNNNNTGG 1 cut(s) 446
BstYI RGATCY 3 cut(s) 349, 1035, 1320
Bsu15I ATCGAT 2 cut(s) 278, 847
Bsu36I CCTNAGG 2 cut(s) 1308, 2737
BsuRI GGCC 8 cut(s) 122, 267, 1092, 1285, 2119, 2256, 2742, 2819
BsuTUI ATCGAT 2 cut(s) 278, 847
BtgI CCRYGG 2 cut(s) 945, 1863
BtgZI GCGATG 1 cut(s) 2533
BtrI CACGTC 1 cut(s) 2127
BtsCI GGATG 6 cut(s) 435, 455, 709, 1133, 1319, 1932
BtsI GCAGTG 2 cut(s) 54, 1584
BtsIMutI CAGTG 8 cut(s) 54, 94, 201, 576, 791, 1584, 1721, 1776
BtuMI TCGCGA 1 cut(s) 2552
BveI ACCTGC 1 cut(s) 2726
Cac8I GCNNGC 3 cut(s) 255, 285, 1528
CaiI CAGNNNCTG 2 cut(s) 218, 2718
CfoI GCGC 1 cut(s) 2389
Cfr10I RCCGGY 1 cut(s) 140
ClaI ATCGAT 2 cut(s) 278, 847
CseI GACGC 1 cut(s) 899
Csp6I GTAC 6 cut(s) 753, 1077, 1432, 1474, 1518, 2053
CspCI CAANNNNNGTGG 4 cut(s) 30, 65, 1893, 1928
CviQI GTAC 6 cut(s) 753, 1077, 1432, 1474, 1518, 2053
DpnI GATC 8 cut(s) 303, 351, 1020, 1027, 1037, 1322, 2025, 2103
DpnII GATC 8 cut(s) 301, 349, 1018, 1025, 1035, 1320, 2023, 2101
DraIII CACNNNGTG 3 cut(s) 89, 797, 1202
Eam1104I CTCTTC 3 cut(s) 163, 2471, 2505
EarI CTCTTC 3 cut(s) 163, 2471, 2505
Ecl136II GAGCTC 2 cut(s) 601, 2783
Eco130I CCWWGG 4 cut(s) 678, 945, 1863, 2814
Eco147I AGGCCT 1 cut(s) 1092
Eco24I GRGCYC 2 cut(s) 603, 2785
Eco31I GGTCTC 2 cut(s) 2488, 2607
Eco47I GGWCC 7 cut(s) 583, 1049, 1241, 1271, 1611, 1814, 2811
Eco53kI GAGCTC 2 cut(s) 601, 2783
Eco57I CTGAAG 3 cut(s) 750, 1982, 2796
Eco81I CCTNAGG 2 cut(s) 1308, 2737
Eco88I CYCGRG 2 cut(s) 1562, 2652
EcoICRI GAGCTC 2 cut(s) 601, 2783
EcoNI CCTNNNNNAGG 1 cut(s) 2736
EcoO109I RGGNCCY 1 cut(s) 1611
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 4 cut(s) 164, 318, 2334, 2848
EcoT14I CCWWGG 4 cut(s) 678, 945, 1863, 2814
EcoT38I GRGCYC 2 cut(s) 603, 2785
ErhI CCWWGG 4 cut(s) 678, 945, 1863, 2814
FalI AAGNNNNNCTT 2 cut(s) 1228, 1260
FaqI GGGAC 5 cut(s) 13, 33, 200, 530, 979
FblI GTMKAC 1 cut(s) 477
FokI GGATG 6 cut(s) 422, 462, 716, 1120, 1326, 1939
FriOI GRGCYC 2 cut(s) 603, 2785
FspBI CTAG 3 cut(s) 627, 1404, 2130
GlaI GCGC 1 cut(s) 2388
GsaI CCCAGC 1 cut(s) 1431
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 8 cut(s) 122, 267, 1092, 1285, 2119, 2256, 2742, 2819
HapII CCGG 4 cut(s) 141, 1287, 2092, 2390
HgaI GACGC 1 cut(s) 899
HhaI GCGC 1 cut(s) 2389
Hin6I GCGC 1 cut(s) 2387
HinP1I GCGC 1 cut(s) 2387
HincII GTYRAC 3 cut(s) 738, 1737, 1983
HindII GTYRAC 3 cut(s) 738, 1737, 1983
HindIII AAGCTT 1 cut(s) 973
HpaI GTTAAC 2 cut(s) 738, 1983
HpaII CCGG 4 cut(s) 141, 1287, 2092, 2390
HphI GGTGA 9 cut(s) 427, 1175, 1900, 2295, 2406, 2573, 2691, 2777, 2810
Hpy188I TCNGA 8 cut(s) 130, 193, 2085, 2267, 2407, 2428, 2749, 2830
Hpy99I CGWCG 2 cut(s) 893, 2198
HpyCH4III ACNGT 7 cut(s) 205, 1793, 1840, 2063, 2438, 2708, 2836
HpyCH4IV ACGT 5 cut(s) 180, 903, 1434, 2126, 2145
HpySE526I ACGT 5 cut(s) 180, 903, 1434, 2126, 2145
HspAI GCGC 1 cut(s) 2387
KspAI GTTAAC 2 cut(s) 738, 1983
Kzo9I GATC 8 cut(s) 301, 349, 1018, 1025, 1035, 1320, 2023, 2101
LguI GCTCTTC 1 cut(s) 2505
LmnI GCTCC 4 cut(s) 2627, 2654, 2678, 2780
MaeI CTAG 3 cut(s) 627, 1404, 2130
MaeII ACGT 5 cut(s) 180, 903, 1434, 2126, 2145
MaeIII GTNAC 5 cut(s) 1955, 2112, 2141, 2561, 2708
MalI GATC 8 cut(s) 303, 351, 1020, 1027, 1037, 1322, 2025, 2103
MboI GATC 8 cut(s) 301, 349, 1018, 1025, 1035, 1320, 2023, 2101
MfeI CAATTG 2 cut(s) 1029, 1179
MflI RGATCY 3 cut(s) 349, 1035, 1320
MhlI GDGCHC 7 cut(s) 327, 468, 603, 645, 793, 816, 2785
MlyI GAGTC 7 cut(s) 182, 398, 639, 1394, 1975, 2470, 2558
MmeI TCCRAC 4 cut(s) 902, 1249, 2457, 2853
MroXI GAANNNNTTC 2 cut(s) 2018, 2859
MseI TTAA 6 cut(s) 114, 737, 1469, 1709, 1982, 2526
MslI CAYNNNNRTG 2 cut(s) 116, 2538
MspA1I CMGCKG 2 cut(s) 215, 2774
MspI CCGG 4 cut(s) 141, 1287, 2092, 2390
MspR9I CCNGG 6 cut(s) 166, 320, 1287, 2093, 2336, 2850
MunI CAATTG 2 cut(s) 1029, 1179
Mva1269I GAATGC 1 cut(s) 247
MvaI CCWGG 4 cut(s) 166, 320, 2336, 2850
MvnI CGCG 4 cut(s) 1812, 2076, 2387, 2552
NciI CCSGG 2 cut(s) 1287, 2093
NcoI CCATGG 2 cut(s) 945, 1863
NdeII GATC 8 cut(s) 301, 349, 1018, 1025, 1035, 1320, 2023, 2101
NlaIV GGNNCC 2 cut(s) 324, 2650
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 2 cut(s) 2141, 2561
NruI TCGCGA 1 cut(s) 2552
NspI RCATGY 3 cut(s) 649, 2403, 2543
NspV TTCGAA 1 cut(s) 370
PaqCI CACCTGC 1 cut(s) 2726
PceI AGGCCT 1 cut(s) 1092
PciI ACATGT 2 cut(s) 645, 2399
PciSI GCTCTTC 1 cut(s) 2505
PcsI WCGNNNNNNNCGW 2 cut(s) 489, 2067
PctI GAATGC 1 cut(s) 247
PdmI GAANNNNTTC 2 cut(s) 2018, 2859
PfeI GAWTC 9 cut(s) 64, 80, 874, 1292, 1417, 1646, 1726, 2429, 2696
PflFI GACNNNGTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 1202
PleI GAGTC 7 cut(s) 182, 398, 638, 1394, 1974, 2469, 2558
PpsI GAGTC 7 cut(s) 182, 398, 638, 1394, 1974, 2469, 2558
PpuMI RGGWCCY 1 cut(s) 1611
PscI ACATGT 2 cut(s) 645, 2399
PshAI GACNNNNGTC 2 cut(s) 25, 629
PsiI TTATAA 1 cut(s) 2030
Psp124BI GAGCTC 2 cut(s) 603, 2785
Psp1406I AACGTT 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 1611
Psp6I CCWGG 4 cut(s) 164, 318, 2334, 2848
PspFI CCCAGC 1 cut(s) 1427
PspGI CCWGG 4 cut(s) 164, 318, 2334, 2848
PspN4I GGNNCC 2 cut(s) 324, 2650
PspPPI RGGWCCY 1 cut(s) 1611
PstI CTGCAG 1 cut(s) 223
PstNI CAGNNNCTG 2 cut(s) 218, 2718
PsuI RGATCY 3 cut(s) 349, 1035, 1320
PsyI GACNNNGTC 1 cut(s) 203
PvuII CAGCTG 2 cut(s) 215, 2774
RruI TCGCGA 1 cut(s) 2552
RsaI GTAC 6 cut(s) 754, 1078, 1433, 1475, 1519, 2054
RsaNI GTAC 6 cut(s) 753, 1077, 1432, 1474, 1518, 2053
RseI CAYNNNNRTG 2 cut(s) 116, 2538
SacI GAGCTC 2 cut(s) 603, 2785
SapI GCTCTTC 1 cut(s) 2505
SaqAI TTAA 6 cut(s) 114, 737, 1469, 1709, 1982, 2526
Sau3AI GATC 8 cut(s) 301, 349, 1018, 1025, 1035, 1320, 2023, 2101
SchI GAGTC 7 cut(s) 182, 398, 639, 1394, 1975, 2470, 2558
ScrFI CCNGG 6 cut(s) 166, 320, 1287, 2093, 2336, 2850
SduI GDGCHC 7 cut(s) 327, 468, 603, 645, 793, 816, 2785
SfcI CTRYAG 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 370
SinI GGWCC 7 cut(s) 583, 1049, 1241, 1271, 1611, 1814, 2811
SmiMI CAYNNNNRTG 2 cut(s) 116, 2538
SmlI CTYRAG 2 cut(s) 246, 1989
SmoI CTYRAG 2 cut(s) 246, 1989
SseBI AGGCCT 1 cut(s) 1092
SsiI CCGC 6 cut(s) 393, 1812, 2074, 2175, 2487, 2840
SspI AATATT 1 cut(s) 2231
SspMI CTAG 3 cut(s) 627, 1404, 2130
SstI GAGCTC 2 cut(s) 603, 2785
StuI AGGCCT 1 cut(s) 1092
StyD4I CCNGG 6 cut(s) 164, 318, 1285, 2091, 2334, 2848
StyI CCWWGG 4 cut(s) 678, 945, 1863, 2814
TaaI ACNGT 7 cut(s) 205, 1793, 1840, 2063, 2438, 2708, 2836
TaiI ACGT 5 cut(s) 183, 906, 1437, 2129, 2148
TaqI TCGA 9 cut(s) 207, 278, 370, 553, 847, 888, 2022, 2193, 2691
TatI WGTACW 2 cut(s) 752, 1473
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 9 cut(s) 64, 80, 874, 1292, 1417, 1646, 1726, 2429, 2696
Tru1I TTAA 6 cut(s) 114, 737, 1469, 1709, 1982, 2526
Tru9I TTAA 6 cut(s) 114, 737, 1469, 1709, 1982, 2526
TscAI CASTG 8 cut(s) 54, 94, 208, 583, 798, 1584, 1728, 1776
TseFI GTSAC 2 cut(s) 2141, 2561
Tsp45I GTSAC 2 cut(s) 2141, 2561
TspDTI ATGAA 8 cut(s) 17, 625, 720, 1409, 1483, 1818, 1965, 2536
TspGWI ACGGA 1 cut(s) 1126
TspRI CASTG 8 cut(s) 54, 94, 208, 583, 798, 1584, 1728, 1776
Tth111I GACNNNGTC 1 cut(s) 203
Van91I CCANNNNNTGG 1 cut(s) 1202
VneI GTGCAC 2 cut(s) 641, 789
VpaK11BI GGWCC 7 cut(s) 583, 1049, 1241, 1271, 1611, 1814, 2811
XagI CCTNNNNNAGG 1 cut(s) 2736
XapI RAATTY 5 cut(s) 4, 290, 1082, 1567, 1805
XceI RCATGY 3 cut(s) 649, 2403, 2543
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 2 cut(s) 2018, 2859
XspI CTAG 3 cut(s) 627, 1404, 2130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.