Rh6DG499100

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000051
Physical Location & Seq
Reverse (-)
65596636 .. 65612816
16181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG499100.1

Sequence Viewer

Length: 2871 bp
ATGGAGAATCCTACCAACATGTTTTTTCTTCTCTTCTTGTTCTTCAGGATTTCCCTGGCATTGGCACAGAACATAACAATTCCAATAAATGTGGGTGTGGTTCTTGACTTGGATACCCAATTTGGCAAGATGGGGCTAAGCTGCATCAACATGGGCCTCTCTGATTTCTATGCCTCTCATGCTTACTACAGTACTAGGCTTGTCCTACACACCAGGGACTCCTCGAAAGATGTTGTTGTTGCAGCTGGTGCAGCTCTAGAGTTGATAAACAATGTTAAAGTGCAAGCCATCATAGGGCCACAATCATCAATGCAAGCAAGTTTTGTAACTGAACTTGGAGATAAAGCACAGGTTCCGATAATCTCATTTTCAGCTACAAGCTCTTCCCTTCAGAGTTCATATTTTCTCCGAATTGCACAAAACGACACATCTCAAGTCAAAGCCATAAGCGACCTCATCCAAGCTTTTAGTTGGAGTGAAGCAGTGCCCATCAGCGTTGACAACGAGTTTGGGAAGGGAATGATACCATATCTGACTACTGCTTTGCAAGCCATTGGTGCTCGCATCCCCTACTGGGTTGTCATTCCTTCACTGGCCACTGCTGACCAAATAGATGAAGAGCTTGAGAAGTTGATGAAAATGCAAACTAGGGTCTTCATAGTGCATATGTTGCCTTCACTTGGCTCTCGACTTTTTGCAAGGGCAAAAAAGTTGGGAATGATGGATGAAGGCTTTGCATGGATAATTACAAATGGGATGACTAACTATTTCAGTTCCGTAAATTCATCTTTCATAGATGACATGAATGGGGTGTTGGGTTTAAAGGCTTATATTCCCAAAACGAAAGAGCTCGGAAACTTTAGGGGTAGATGGCAAAGGAACTTCCAACAAGACAATTCAACTCTCCCGAGTGTCAACTTGGATGTTTTTGGATTCTGGGCCTATGATGCTGCATGGGCACTAGCCATGGCAGTTGAAAATGTTGACACTGCAAGCTTCAAGTTCCAAAACACGAACATTTCTGGCAGTTCCACTGGTTTAGAAAGATTGGAGATCTCTCTAAGTGGCCCTGAACTTGTCCGAGAATTATCAAGTACAAGGTTCATAGGAATTTCTGGAGATTTCAGTCTTCTTGATGGGCAACTGCAGTCATCAACTTTTGAGGTGGTTAATGTGAACAATAATGGGGAAAGAGGAATTGGATATTGGACACCCCGAAATGGATTTGTAAGAAACTTAAATTCAGAAAACGCAAGTACATATTCTACTTCCAATGCCAGTCTTGGTCCTATTATATGGCCGGGGGAGACCACCTCGGCTCCAAAAGGTTGGCAGATTCCTACTGGGGCAAAACTGAAGGTTCTAATCCCGGTCAAGACAGGTTTTACAGAATTTGTAAATATAAGTACAGATCCAAACACTCACACAACTGATATCACTGGATACGGCATAGAAGTCTTTAATGCTGTAACAAAATCATTACCATTCCCTGTTCCTTACGAGTTTCATCCCTTTGCAAAGCCTAATGGTGAGAGCGCCGGTAGTTACAATGATTTACTTGATCAAGTGTTTCTTGGGAATTATGATGCTGCAGTTGGAGACATAACCATCAGAGCAAATAGATCTTTATATGTTGACTTTACATTACCATACACAGAATCTGGGATATCAATGATAGTGCCTATCAAAGATCATAAAGTTGCTAAAACCACATGGGTGTTCTTGAAGCCTTTCACTTTGGAACTTTGGGTAACAAGCGGTTGTTTTTTCATATTCATTGGTTCTGTAGTCTGGGTTCTCGAACATCGGATAAACGAAGACTTTCGGGGGCCTCCATGTCACCAAATGGGCACAAGCTTGTGGTACTCATTCTCAACCATGGTCTTTGCACACCGGGAGCGAGTAATGAGCAACTTGGCTAGGTTTGTCGTGATCACATGGTGCTTTGTTGTTCTCATACTGACTCAAAGTTACACTGCCAGTTTCAGTTCAATTTTGACAGTTCAACAGCTCCAAAAGAACAGTACCGATGTAAACCAGCTCCTGAAGATTGGGGAAAACGTGGGTTACCAATTGGGTTCTTTTGTTCTGGGGACCTTGCAGCATCTTGGTTTTCGGGATGACAAACTTAGGGTGTATAATACTGTGGAAGAATTGAATAAACTTCTTCAAATGGGAAGTGAAAATAATGGTATTTCAGCTGCTTTTGATGAAACCCCCTACATGAAACTGTTTCTTGCAACTTATTGCTCAAAATATACCATGGTTGAGCCAACATTTAAAGCTGATGGCTTTGCCTTTGCCTTCCAAAAAGGTTCACTCCTCACACGTTATGTTTCAAGGGAAATCGAGGGAACTGATCTGACGAAAATTGAGGAAAAATGGCTCGTTAAAACTACAAGTTGTTCAGACTCCAATGCAGGGGACTCTTCCAACAATACTCTAACAGTTGATAGCTTTTGGGGGCTCTTTATCATTGCTGGGGTTGCTTCATCATTAGCTCTCCTCATATTTGCAGCCATGTTCTTTTATGAGCACAGGCACACCTTGCTGAACCTTGATCCGGAAACCACATTTTGGGGAAGAATTCGTGTGATATTGAGGATATATGACCAAAGAGACCCCACTTCGCACACTTACAACAGGAGAGGACTGCAAGATCCTGTTCATGGTCTAAGTGCAGTTGAATCCTTACCAGACACTAATTCTTCCGCGAGGGCAAGTGCGAACCGCATTGACTCACACATTGATCTTGAACAGCATGTCCGGACTGAGCATGCTGACTTGAATCCCAACACTCAAGCAACCCAAGAGATTGAAGATGCAATTGACTTGACCTCCTCCAGTGAACAGAGGCCAGTGCCTCGTTAG

Protein Analysis

956

Amino Acids

106.35

Weight (kDa)

5.46

Isoelectric Point (pI)

29.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 28 - 334 2.4e-17 Periplasmic binding protein
ANF_receptor PF01094 48 - 394 3.9e-75 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 473 - 561 2.6e-09 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 477 - 710 3.5e-06 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 580 - 830 5.4e-36 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24710 AT2G24710 AT2G24710 AT2G24720 AT2G29100 AT2G29100 AT2G29110 AT2G29110 AT2G29120 AT5G27100
fragaria_vesca FvH4_2g11260 FvH4_2g11280 FvH4_2g11310 FvH4_2g11310 FvH4_2g11331 FvH4_2g11331 FvH4_2g11340 FvH4_2g11340 FvH4_2g11340 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31990 FvH4_2g37140 FvH4_2g37150 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37161 FvH4_2g37161 FvH4_2g37163 FvH4_2g37163 FvH4_2g37170 FvH4_2g37181 FvH4_2g37191 FvH4_3g22781 FvH4_3g22781 FvH4_3g22781 FvH4_3g22790 FvH4_3g22790 FvH4_3g22810 FvH4_5g13460 FvH4_5g34751 FvH4_7g09130 FvH4_7g27991 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010
malus_domestica MD08G1047600.v1.1 MD08G1047700.v1.1 MD08G1048100.v1.1 MD08G1048200.v1.1 MD08G1048300.v1.1 MD08G1048500.v1.1 MD08G1048600.v1.1 MD08G1048700.v1.1 MD08G1048900.v1.1 MD08G1049000.v1.1 MD14G1064700.v1.1 MD15G1045900.v1.1 MD15G1046000.v1.1 MD15G1046200.v1.1 MD15G1046400.v1.1 MD15G1059200.v1.1 MD15G1059500.v1.1 MD15G1059600.v1.1 MD15G1059700.v1.1
prunus_persica Prupe.1G399500_v2.0.a1 Prupe.1G399600_v2.0.a1 Prupe.1G399700_v2.0.a1 Prupe.1G399800_v2.0.a1 Prupe.1G399900_v2.0.a1 Prupe.1G400000_v2.0.a1 Prupe.1G400300_v2.0.a1 Prupe.1G400400_v2.0.a1 Prupe.1G400500_v2.0.a1 Prupe.1G400600_v2.0.a1 Prupe.1G400700_v2.0.a1 Prupe.1G412700_v2.0.a1 Prupe.1G412800_v2.0.a1
pyrus_communis pycom02g01350 pycom08g04020 pycom08g04040 pycom08g04080 pycom08g04090 pycom08g04100 pycom14g05240 pycom15g04300 pycom15g04310 pycom15g04330 pycom15g04340 pycom15g04380 pycom15g05560 pycom15g05570 pycom15g05580 pycom15g05600
rosa_chinensis RchiOBHm_Chr1g0345071 RchiOBHm_Chr1g0345081 RchiOBHm_Chr1g0345171 RchiOBHm_Chr3g0480811 RchiOBHm_Chr3g0492581 RchiOBHm_Chr5g0020871 RchiOBHm_Chr5g0039801 RchiOBHm_Chr5g0039811 RchiOBHm_Chr5g0039861 RchiOBHm_Chr5g0039911 RchiOBHm_Chr5g0039941 RchiOBHm_Chr5g0039951 RchiOBHm_Chr5g0040011 RchiOBHm_Chr6g0269601 RchiOBHm_Chr6g0269621 RchiOBHm_Chr6g0269631 RchiOBHm_Chr6g0269641 RchiOBHm_Chr6g0269671 RchiOBHm_Chr6g0269681 RchiOBHm_Chr6g0269731 RchiOBHm_Chr6g0269751 RchiOBHm_Chr6g0269761 RchiOBHm_Chr6g0269771 RchiOBHm_Chr6g0269781 RchiOBHm_Chr6g0269861 RchiOBHm_Chr6g0269871 RchiOBHm_Chr6g0269881 RchiOBHm_Chr6g0269911 RchiOBHm_Chr6g0269921 RchiOBHm_Chr6g0269941 RchiOBHm_Chr6g0269951 RchiOBHm_Chr6g0304511 RchiOBHm_Chr6g0304621 RchiOBHm_Chr6g0304641 RchiOBHm_Chr6g0304651 RchiOBHm_Chr6g0304661 RchiOBHm_Chr6g0310781 RchiOBHm_Chr6g0310801 RchiOBHm_Chr6g0310811 RchiOBHm_Chr6g0310821 RchiOBHm_Chr6g0310831 RchiOBHm_Chr7g0183621 RchiOBHm_Chr7g0183651
rosa_laevigata RLG00000005035 RLG00000005036 RLG00000010458 RLG00000010459 RLG00000010460 RLG00000010961 RLG00000010962 RLG00000010963 RLG00000010964 RLG00000010966 RLG00000010970 RLG00000013824 RLG00000013825 RLG00000013826 RLG00000013828 RLG00000013829 RLG00000013831 RLG00000013833 RLG00000013835 RLG00000013837 RLG00000013840 RLG00000013842 RLG00000013844 RLG00000022758 RLG00000023449 RLG00000026705 RLG00000028849 RLG00000028853 RLG00000028854 RLG00000032569 RLG00000033944 RLG00000033945 RLG00000033947 RLG00000033949 RLG00000033951 RLG00000034239
rosa_multiflora Rmu_co8287891.1_g000001 Rmu_co8428745.1_g000001 Rmu_co8521767.1_g000002 Rmu_sc0000103.1_g000022 Rmu_sc0000103.1_g000031 Rmu_sc0000103.1_g000041 Rmu_sc0000103.1_g000053 Rmu_sc0000247.1_g000028 Rmu_sc0000247.1_g000051 Rmu_sc0000893.1_g000003 Rmu_sc0000893.1_g000017 Rmu_sc0001143.1_g000008 Rmu_sc0001143.1_g000019 Rmu_sc0001143.1_g000023 Rmu_sc0001975.1_g000003 Rmu_sc0001975.1_g000004 Rmu_sc0001975.1_g000005 Rmu_sc0001975.1_g000012 Rmu_sc0002151.1_g000006 Rmu_sc0003610.1_g000021 Rmu_sc0003682.1_g000001 Rmu_sc0003863.1_g000004 Rmu_sc0003863.1_g000007 Rmu_sc0003863.1_g000013 Rmu_sc0004196.1_g000005 Rmu_sc0004515.1_g000013 Rmu_sc0004515.1_g000024 Rmu_sc0004688.1_g000001 Rmu_sc0004688.1_g000003 Rmu_sc0004688.1_g000004 Rmu_sc0004748.1_g000006 Rmu_sc0004838.1_g000016 Rmu_sc0004838.1_g000017 Rmu_sc0005532.1_g000006 Rmu_sc0005532.1_g000011 Rmu_sc0005950.1_g000004 Rmu_sc0006576.1_g000005 Rmu_sc0006576.1_g000021 Rmu_sc0008520.1_g000002 Rmu_sc0008520.1_g000004 Rmu_sc0009130.1_g000002 Rmu_sc0018719.1_g000001 Rmu_sc0027713.1_g000005 Rmu_sc0040804.1_g000001 Rmu_ssc0000218.1_g000059 Rmu_ssc0000218.1_g000062 Rmu_ssc0000218.1_g000068 Rmu_ssc0000395.1_g000008 Rmu_ssc0000395.1_g000009 Rmu_ssc0000395.1_g000010 Rmu_ssc0000395.1_g000011 Rmu_ssc0000395.1_g000013 Rmu_ssc0000395.1_g000014
rosa_roxburghii Rroxscaffold_1G00041270 Rroxscaffold_1G00041290 Rroxscaffold_1G00041300 Rroxscaffold_1G00041360 Rroxscaffold_1G00041380 Rroxscaffold_1G00057500 Rroxscaffold_3G00270350 Rroxscaffold_4G00283160 Rroxscaffold_4G00309210 Rroxscaffold_4G00309240 Rroxscaffold_4G00309260 Rroxscaffold_4G00309290 Rroxscaffold_4G00309360 Rroxscaffold_6G00392210 Rroxscaffold_6G00401030 Rroxscaffold_7G00158260 Rroxscaffold_7G00163620 Rroxscaffold_7G00163630 Rroxscaffold_7G00163650 Rroxscaffold_7G00163680 Rroxscaffold_7G00198270 Rroxscaffold_7G00198280 Rroxscaffold_7G00198330 Rroxscaffold_7G00198340 Rroxscaffold_7G00198360 Rroxscaffold_7G00198390 Rroxscaffold_7G00198410 Rroxscaffold_7G00198450 Rroxscaffold_7G00198460 Rroxscaffold_7G00198490 Rroxscaffold_7G00198500 Rroxscaffold_7G00198510 Rroxscaffold_7G00198530 Rroxscaffold_7G00198550 Rroxscaffold_7G00198600
rosa_rugosa Rorug01G0175900 Rorug01G0176200 Rorug03G0186700 Rorug03G0254500 Rorug05G0182700 Rorug05G0183000 Rorug05G0183000 Rorug06G0049000 Rorug06G0049500 Rorug06G0049600.1 Rorug06G0049700 Rorug06G0050000 Rorug06G0050000 Rorug06G0050100 Rorug06G0050300.1 Rorug06G0050400 Rorug06G0051100 Rorug06G0051200 Rorug06G0051300 Rorug06G0051500 Rorug06G0051600 Rorug06G0051700 Rorug06G0332600 Rorug06G0332700 Rorug06G0332700 Rorug06G0383100 Rorug06G0383200 Rorug06G0383300 Rorug06G0383400 Rorug06G0459100 Rorug06G0459200
rosa_samantha Rh1BG160400 Rh1BG160500 Rh1BG160700 Rh3DG263900 Rh3DG338400 Rh5CG164400 Rh5CG164500 Rh5CG305500 Rh5CG305900 Rh5CG306000 Rh5DG280800 Rh5DG281300 Rh6AG445300 Rh6AG497000 Rh6BG174700 Rh6BG457200 Rh6CG168900 Rh6CG169800 Rh6CG512700 Rh6CG512900 Rh6DG034100 Rh6DG160500 Rh6DG160800 Rh6DG160900 Rh6DG161100 Rh6DG161400 Rh6DG161800 Rh6DG162000 Rh6DG162100 Rh6DG162200 Rh6DG444800 Rh6DG445000 Rh6DG445100 Rh6DG445200 Rh6DG445300 Rh6DG499100 Rh6DG499200 Rh6DG499300 Rh6DG499400 Rh6DG499500 Rh6DG499700 Rh7BG059800 Rh7BG060000 Rh7BG258500 Rh7BG258700
rosa_wichuraiana Rw0G006180 Rw0G007950 Rw0G018490 Rw0G022090 Rw1G015970 Rw1G015990 Rw3G021330 Rw5G025120 Rw5G025140 Rw5G025150 Rw5G025160 Rw5G025180 Rw5G025830 Rw5G025850 Rw6G014550 Rw6G014570 Rw6G014580 Rw6G014600 Rw6G014620 Rw6G014650 Rw6G014670 Rw6G038650 Rw6G038670 Rw6G038680 Rw6G038700 Rw6G038710 Rw6G038720 Rw6G043280 Rw6G043290 Rw7G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2577
AccII CGCG 1 cut(s) 2714
AccIII TCCGGA 2 cut(s) 2563, 2766
AciI CCGC 3 cut(s) 1758, 2712, 2731
AclWI GGATC 3 cut(s) 1406, 2555, 2654
AcoI YGGCCR 2 cut(s) 594, 1298
AcsI RAATTY 5 cut(s) 781, 1110, 1240, 1391, 2586
AcuI CTGAAG 4 cut(s) 28, 374, 1376, 2066
AdeI CACNNNGTG 1 cut(s) 1941
AfaI GTAC 6 cut(s) 193, 1096, 1258, 1408, 1865, 2026
AfiI CCNNNNNNNGG 9 cut(s) 61, 294, 574, 680, 1220, 2421, 2563, 2577, 2669
AflIII ACRYGT 2 cut(s) 18, 2327
AjnI CCWGG 2 cut(s) 54, 212
AjuI GAANNNNNNNTTGG 6 cut(s) 797, 829, 1182, 1189, 1214, 1221
AleI CACNNNNGTG 1 cut(s) 1715
AloI GAACNNNNNNTCC 2 cut(s) 2748, 2780
Alw21I GWGCWC 3 cut(s) 562, 852, 2538
Alw26I GTCTC 3 cut(s) 1301, 1593, 2613
AlwI GGATC 3 cut(s) 1406, 2555, 2654
AlwNI CAGNNNCTG 2 cut(s) 1661, 2044
Ama87I CYCGRG 1 cut(s) 907
Aor13HI TCCGGA 2 cut(s) 2563, 2766
AoxI GGCC 8 cut(s) 154, 296, 594, 939, 1066, 1298, 1829, 2855
ApeKI GCWGC 8 cut(s) 141, 242, 251, 950, 1589, 2101, 2201, 2516
ApoI RAATTY 5 cut(s) 781, 1110, 1240, 1391, 2586
ArsI GACNNNNNNTTYG 4 cut(s) 491, 523, 526, 558
Asp700I GAANNNNTTC 2 cut(s) 1730, 1821
AspLEI GCGC 1 cut(s) 1538
AspS9I GGNCC 7 cut(s) 154, 296, 939, 1067, 1286, 1829, 2094
AsuC2I CCSGG 3 cut(s) 1302, 1370, 1895
AsuHPI GGTGA 2 cut(s) 1541, 1832
AvaI CYCGRG 1 cut(s) 907
AvaII GGWCC 2 cut(s) 1286, 2094
BaeGI GKGCMC 3 cut(s) 489, 961, 1853
BalI TGGCCA 1 cut(s) 596
BanII GRGCYC 2 cut(s) 852, 2469
BbsI GAAGAC 3 cut(s) 646, 1121, 1824
Bbv12I GWGCWC 3 cut(s) 562, 852, 2538
BbvI GCAGC 8 cut(s) 128, 254, 263, 937, 1576, 2113, 2188, 2528
BccI CCATC 8 cut(s) 124, 296, 497, 715, 864, 1130, 1616, 2282
BceAI ACGGC 1 cut(s) 1462
BciT130I CCWGG 2 cut(s) 56, 214
BciVI GTATCC 2 cut(s) 106, 1436
BclI TGATCA 2 cut(s) 1561, 1932
BcnI CCSGG 3 cut(s) 1302, 1370, 1895
BcoDI GTCTC 3 cut(s) 1301, 1593, 2613
BfaI CTAG 5 cut(s) 195, 257, 648, 962, 1920
BfmI CTRYAG 4 cut(s) 187, 1145, 1590, 1785
BfoI RGCGCY 1 cut(s) 1539
BfuI GTATCC 2 cut(s) 106, 1436
BglII AGATCT 2 cut(s) 1053, 1622
BisI GCNGC 8 cut(s) 142, 243, 252, 951, 1590, 2102, 2202, 2517
BlpI GCTNAGC 1 cut(s) 137
BlsI GCNGC 8 cut(s) 143, 244, 253, 952, 1591, 2103, 2203, 2518
BmcAI AGTACT 1 cut(s) 193
Bme1390I CCNGG 5 cut(s) 56, 214, 1302, 1370, 1895
Bme18I GGWCC 2 cut(s) 1286, 2094
BmeT110I CYCGRG 1 cut(s) 907
BmgT120I GGNCC 7 cut(s) 154, 296, 939, 1067, 1286, 1829, 2094
BmiI GGNNCC 4 cut(s) 354, 1320, 1830, 2095
BmrFI CCNGG 5 cut(s) 56, 214, 1302, 1370, 1895
BmrI ACTGGG 2 cut(s) 583, 1353
BmsI GCATC 6 cut(s) 153, 573, 937, 1576, 2113, 2812
BmuI ACTGGG 2 cut(s) 583, 1353
BpiI GAAGAC 3 cut(s) 646, 1121, 1824
BplI GAGNNNNNCTC 2 cut(s) 1298, 1330
BpmI CTGGAG 2 cut(s) 1137, 2827
Bpu1102I GCTNAGC 1 cut(s) 137
BpuEI CTTGAG 3 cut(s) 417, 644, 2784
BpuMI CCSGG 3 cut(s) 1302, 1370, 1895
BsaI GGTCTC 2 cut(s) 1301, 2613
BsaJI CCNNGG 7 cut(s) 54, 213, 966, 1301, 1314, 1878, 2262
BsaWI WCCGGW 2 cut(s) 2563, 2766
BsaXI ACNNNNNCTCC 4 cut(s) 1303, 1333, 2822, 2852
Bsc4I CCNNNNNNNGG 9 cut(s) 61, 294, 574, 680, 1220, 2421, 2563, 2577, 2669
Bse118I RCCGGY 1 cut(s) 1538
Bse1I ACTGG 9 cut(s) 578, 597, 1039, 1278, 1348, 1444, 1980, 2844, 2858
Bse3DI GCAATG 1 cut(s) 2475
BseAI TCCGGA 2 cut(s) 2563, 2766
BseBI CCWGG 2 cut(s) 56, 214
BseDI CCNNGG 7 cut(s) 54, 213, 966, 1301, 1314, 1878, 2262
BseGI GGATG 7 cut(s) 456, 564, 730, 762, 928, 1507, 2125
BseLI CCNNNNNNNGG 9 cut(s) 61, 294, 574, 680, 1220, 2421, 2563, 2577, 2669
BseMI GCAATG 1 cut(s) 2475
BseMII CTCAG 1 cut(s) 2763
BseNI ACTGG 9 cut(s) 578, 597, 1039, 1278, 1348, 1444, 1980, 2844, 2858
BseRI GAGGAG 4 cut(s) 211, 2312, 2495, 2830
BseSI GKGCMC 3 cut(s) 489, 961, 1853
BseXI GCAGC 8 cut(s) 128, 254, 263, 937, 1576, 2113, 2188, 2528
BseYI CCCAGC 1 cut(s) 2480
BsgI GTGCAG 2 cut(s) 270, 2700
Bsh1236I CGCG 1 cut(s) 2714
BshFI GGCC 8 cut(s) 156, 298, 596, 941, 1068, 1300, 1831, 2857
BsiHKAI GWGCWC 3 cut(s) 562, 852, 2538
BsiHKCI CYCGRG 1 cut(s) 907
BsiSI CCGG 6 cut(s) 1301, 1370, 1539, 1894, 2564, 2767
BslFI GGGAC 3 cut(s) 230, 2107, 2438
BslI CCNNNNNNNGG 9 cut(s) 61, 294, 574, 680, 1220, 2421, 2563, 2577, 2669
BsmAI GTCTC 3 cut(s) 1301, 1593, 2613
BsmFI GGGAC 3 cut(s) 230, 2107, 2438
BsnI GGCC 8 cut(s) 156, 298, 596, 941, 1068, 1300, 1831, 2857
Bso31I GGTCTC 2 cut(s) 1301, 2613
BsoBI CYCGRG 1 cut(s) 907
Bsp1286I GDGCHC 7 cut(s) 489, 562, 852, 961, 1853, 2469, 2538
Bsp13I TCCGGA 2 cut(s) 2563, 2766
Bsp1720I GCTNAGC 1 cut(s) 137
Bsp19I CCATGG 3 cut(s) 966, 1878, 2262
BspACI CCGC 3 cut(s) 1758, 2712, 2731
BspANI GGCC 8 cut(s) 156, 298, 596, 941, 1068, 1300, 1831, 2857
BspCNI CTCAG 1 cut(s) 2764
BspEI TCCGGA 2 cut(s) 2563, 2766
BspFNI CGCG 1 cut(s) 2714
BspLI GGNNCC 4 cut(s) 354, 1320, 1830, 2095
BspMAI CTGCAG 2 cut(s) 1149, 1594
BspPI GGATC 3 cut(s) 1406, 2555, 2654
BspQI GCTCTTC 2 cut(s) 388, 612
BspTNI GGTCTC 2 cut(s) 1301, 2613
BsrDI GCAATG 1 cut(s) 2475
BsrFI RCCGGY 1 cut(s) 1538
BsrI ACTGG 9 cut(s) 578, 597, 1039, 1278, 1348, 1444, 1980, 2844, 2858
BssAI RCCGGY 1 cut(s) 1538
BssECI CCNNGG 7 cut(s) 54, 213, 966, 1301, 1314, 1878, 2262
BssT1I CCWWGG 3 cut(s) 966, 1878, 2262
Bst2UI CCWGG 2 cut(s) 56, 214
Bst4CI ACNGT 6 cut(s) 191, 2002, 2024, 2146, 2232, 2449
Bst6I CTCTTC 4 cut(s) 38, 388, 612, 2434
BstAPI GCANNNNNTGC 3 cut(s) 248, 670, 2548
BstC8I GCNNGC 6 cut(s) 285, 315, 549, 562, 994, 2778
BstDEI CTNAG 5 cut(s) 137, 1061, 2129, 2675, 2772
BstDSI CCRYGG 3 cut(s) 966, 1878, 2262
BstEII GGTNACC 1 cut(s) 2066
BstF5I GGATG 7 cut(s) 456, 564, 730, 762, 928, 1507, 2125
BstFNI CGCG 1 cut(s) 2714
BstH2I RGCGCY 1 cut(s) 1539
BstHHI GCGC 1 cut(s) 1538
BstMAI GTCTC 3 cut(s) 1301, 1593, 2613
BstNI CCWGG 2 cut(s) 56, 214
BstNSI RCATGY 3 cut(s) 22, 2765, 2780
BstPI GGTNACC 1 cut(s) 2066
BstSCI CCNGG 5 cut(s) 54, 212, 1300, 1368, 1893
BstSFI CTRYAG 4 cut(s) 187, 1145, 1590, 1785
BstSLI GKGCMC 3 cut(s) 489, 961, 1853
BstUI CGCG 1 cut(s) 2714
BstV1I GCAGC 8 cut(s) 128, 254, 263, 937, 1576, 2113, 2188, 2528
BstV2I GAAGAC 3 cut(s) 646, 1121, 1824
BstX2I RGATCY 4 cut(s) 1053, 1411, 1622, 2659
BstXI CCANNNNNNTGG 1 cut(s) 1329
BstYI RGATCY 4 cut(s) 1053, 1411, 1622, 2659
BsuI GTATCC 2 cut(s) 106, 1436
BsuRI GGCC 8 cut(s) 156, 298, 596, 941, 1068, 1300, 1831, 2857
BtgI CCRYGG 3 cut(s) 966, 1878, 2262
BtsCI GGATG 7 cut(s) 456, 564, 730, 762, 928, 1507, 2125
BtsI GCAGTG 4 cut(s) 489, 597, 987, 1974
BtsIMutI CAGTG 9 cut(s) 489, 590, 597, 987, 1032, 1437, 1974, 2851, 2865
Cac8I GCNNGC 6 cut(s) 285, 315, 549, 562, 994, 2778
CaiI CAGNNNCTG 2 cut(s) 1661, 2044
CfoI GCGC 1 cut(s) 1538
Cfr10I RCCGGY 1 cut(s) 1538
Cfr13I GGNCC 7 cut(s) 154, 296, 939, 1067, 1286, 1829, 2094
Csp6I GTAC 6 cut(s) 192, 1095, 1257, 1407, 1864, 2025
CspCI CAANNNNNGTGG 2 cut(s) 72, 107
CviQI GTAC 6 cut(s) 192, 1095, 1257, 1407, 1864, 2025
DdeI CTNAG 5 cut(s) 137, 1061, 2129, 2675, 2772
DraI TTTAAA 2 cut(s) 822, 2281
DraIII CACNNNGTG 1 cut(s) 1941
EaeI YGGCCR 2 cut(s) 594, 1298
Eam1104I CTCTTC 4 cut(s) 38, 388, 612, 2434
EarI CTCTTC 4 cut(s) 38, 388, 612, 2434
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 3 cut(s) 966, 1878, 2262
Eco24I GRGCYC 2 cut(s) 852, 2469
Eco31I GGTCTC 2 cut(s) 1301, 2613
Eco32I GATATC 2 cut(s) 1435, 1668
Eco47I GGWCC 2 cut(s) 1286, 2094
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 4 cut(s) 28, 374, 1376, 2066
Eco88I CYCGRG 1 cut(s) 907
Eco91I GGTNACC 1 cut(s) 2066
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 2 cut(s) 1829, 2094
EcoO65I GGTNACC 1 cut(s) 2066
EcoRI GAATTC 1 cut(s) 2586
EcoRII CCWGG 2 cut(s) 54, 212
EcoRV GATATC 2 cut(s) 1435, 1668
EcoT14I CCWWGG 3 cut(s) 966, 1878, 2262
EcoT38I GRGCYC 2 cut(s) 852, 2469
ErhI CCWWGG 3 cut(s) 966, 1878, 2262
FalI AAGNNNNNCTT 2 cut(s) 1557, 1589
FaqI GGGAC 3 cut(s) 230, 2107, 2438
FauNDI CATATG 1 cut(s) 666
FbaI TGATCA 2 cut(s) 1561, 1932
Fnu4HI GCNGC 8 cut(s) 142, 243, 252, 951, 1590, 2102, 2202, 2517
FokI GGATG 7 cut(s) 443, 551, 737, 769, 935, 1494, 2132
FriOI GRGCYC 2 cut(s) 852, 2469
Fsp4HI GCNGC 8 cut(s) 142, 243, 252, 951, 1590, 2102, 2202, 2517
FspBI CTAG 5 cut(s) 195, 257, 648, 962, 1920
GlaI GCGC 1 cut(s) 1537
GluI GCNGC 8 cut(s) 142, 243, 252, 951, 1590, 2102, 2202, 2517
GsaI CCCAGC 1 cut(s) 2484
GsuI CTGGAG 2 cut(s) 1137, 2827
HaeII RGCGCY 1 cut(s) 1539
HaeIII GGCC 8 cut(s) 156, 298, 596, 941, 1068, 1300, 1831, 2857
HapII CCGG 6 cut(s) 1301, 1370, 1539, 1894, 2564, 2767
HhaI GCGC 1 cut(s) 1538
Hin6I GCGC 1 cut(s) 1536
HinP1I GCGC 1 cut(s) 1536
HincII GTYRAC 4 cut(s) 499, 916, 985, 1636
HindII GTYRAC 4 cut(s) 499, 916, 985, 1636
HindIII AAGCTT 3 cut(s) 462, 994, 1855
HpaII CCGG 6 cut(s) 1301, 1370, 1539, 1894, 2564, 2767
HphI GGTGA 2 cut(s) 1541, 1832
Hpy166II GTNNAC 8 cut(s) 499, 916, 985, 1177, 1636, 2035, 2318, 2849
Hpy8I GTNNAC 8 cut(s) 499, 916, 985, 1177, 1636, 2035, 2318, 2849
HpyAV CCTTC 7 cut(s) 398, 508, 597, 684, 722, 1351, 2314
HpyCH4III ACNGT 6 cut(s) 191, 2002, 2024, 2146, 2232, 2449
HpyCH4IV ACGT 2 cut(s) 2061, 2329
HpyF3I CTNAG 5 cut(s) 137, 1061, 2129, 2675, 2772
HpySE526I ACGT 2 cut(s) 2061, 2329
HspAI GCGC 1 cut(s) 1536
Kpn2I TCCGGA 2 cut(s) 2563, 2766
Ksp22I TGATCA 2 cut(s) 1561, 1932
LguI GCTCTTC 2 cut(s) 388, 612
LmnI GCTCC 4 cut(s) 1324, 1897, 2016, 2046
Lsp1109I GCAGC 8 cut(s) 128, 254, 263, 937, 1576, 2113, 2188, 2528
LweI GCATC 6 cut(s) 153, 573, 937, 1576, 2113, 2812
MaeI CTAG 5 cut(s) 195, 257, 648, 962, 1920
MaeII ACGT 2 cut(s) 2061, 2329
MaeIII GTNAC 7 cut(s) 325, 1468, 1544, 1750, 1838, 1970, 2066
MfeI CAATTG 2 cut(s) 2072, 2826
MflI RGATCY 4 cut(s) 1053, 1411, 1622, 2659
MhlI GDGCHC 7 cut(s) 489, 562, 852, 961, 1853, 2469, 2538
MlsI TGGCCA 1 cut(s) 596
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 5 cut(s) 212, 1957, 2405, 2420, 2732
MmeI TCCRAC 4 cut(s) 452, 910, 1576, 2457
Mox20I TGGCCA 1 cut(s) 596
MroI TCCGGA 2 cut(s) 2563, 2766
MroXI GAANNNNTTC 2 cut(s) 1730, 1821
MscI TGGCCA 1 cut(s) 596
MseI TTAA 7 cut(s) 276, 821, 1170, 1238, 1461, 2280, 2391
MslI CAYNNNNRTG 2 cut(s) 149, 1715
Msp20I TGGCCA 1 cut(s) 596
MspA1I CMGCKG 2 cut(s) 245, 2201
MspI CCGG 6 cut(s) 1301, 1370, 1539, 1894, 2564, 2767
MspR9I CCNGG 5 cut(s) 56, 214, 1302, 1370, 1895
MunI CAATTG 2 cut(s) 2072, 2826
MvaI CCWGG 2 cut(s) 56, 214
MvnI CGCG 1 cut(s) 2714
NciI CCSGG 3 cut(s) 1302, 1370, 1895
NcoI CCATGG 3 cut(s) 966, 1878, 2262
NdeI CATATG 1 cut(s) 666
NlaIV GGNNCC 4 cut(s) 354, 1320, 1830, 2095
NmeAIII GCCGAG 1 cut(s) 1295
NmuCI GTSAC 1 cut(s) 1838
NspI RCATGY 3 cut(s) 22, 2765, 2780
OliI CACNNNNGTG 1 cut(s) 1715
PaeI GCATGC 1 cut(s) 2780
PciI ACATGT 1 cut(s) 18
PciSI GCTCTTC 2 cut(s) 388, 612
PdmI GAANNNNTTC 2 cut(s) 1730, 1821
PfeI GAWTC 6 cut(s) 7, 933, 1336, 1658, 2687, 2788
PflMI CCANNNNNTGG 1 cut(s) 2577
PkrI GCNGC 8 cut(s) 143, 244, 253, 952, 1591, 2103, 2203, 2518
PleI GAGTC 5 cut(s) 212, 1957, 2405, 2420, 2732
PpsI GAGTC 5 cut(s) 212, 1957, 2405, 2420, 2732
PpuMI RGGWCCY 1 cut(s) 2094
PscI ACATGT 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 2094
Psp6I CCWGG 2 cut(s) 54, 212
PspEI GGTNACC 1 cut(s) 2066
PspFI CCCAGC 1 cut(s) 2480
PspGI CCWGG 2 cut(s) 54, 212
PspN4I GGNNCC 4 cut(s) 354, 1320, 1830, 2095
PspPI GGNCC 7 cut(s) 154, 296, 939, 1067, 1286, 1829, 2094
PspPPI RGGWCCY 1 cut(s) 2094
PsrI GAACNNNNNNTAC 2 cut(s) 1779, 1811
PstI CTGCAG 2 cut(s) 1149, 1594
PstNI CAGNNNCTG 2 cut(s) 1661, 2044
PsuI RGATCY 4 cut(s) 1053, 1411, 1622, 2659
PvuII CAGCTG 2 cut(s) 245, 2201
RsaI GTAC 6 cut(s) 193, 1096, 1258, 1408, 1865, 2026
RsaNI GTAC 6 cut(s) 192, 1095, 1257, 1407, 1864, 2025
RseI CAYNNNNRTG 2 cut(s) 149, 1715
SacI GAGCTC 1 cut(s) 852
SapI GCTCTTC 2 cut(s) 388, 612
SaqAI TTAA 7 cut(s) 276, 821, 1170, 1238, 1461, 2280, 2391
SatI GCNGC 8 cut(s) 142, 243, 252, 951, 1590, 2102, 2202, 2517
Sau96I GGNCC 7 cut(s) 154, 296, 939, 1067, 1286, 1829, 2094
ScaI AGTACT 1 cut(s) 193
SchI GAGTC 5 cut(s) 212, 1957, 2405, 2420, 2732
ScrFI CCNGG 5 cut(s) 56, 214, 1302, 1370, 1895
SduI GDGCHC 7 cut(s) 489, 562, 852, 961, 1853, 2469, 2538
SfaNI GCATC 6 cut(s) 153, 573, 937, 1576, 2113, 2812
SfcI CTRYAG 4 cut(s) 187, 1145, 1590, 1785
SinI GGWCC 2 cut(s) 1286, 2094
SmiMI CAYNNNNRTG 2 cut(s) 149, 1715
SmlI CTYRAG 3 cut(s) 432, 623, 2799
SmoI CTYRAG 3 cut(s) 432, 623, 2799
SphI GCATGC 1 cut(s) 2780
SsiI CCGC 3 cut(s) 1758, 2712, 2731
SspMI CTAG 5 cut(s) 195, 257, 648, 962, 1920
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 5 cut(s) 54, 212, 1300, 1368, 1893
StyI CCWWGG 3 cut(s) 966, 1878, 2262
TaaI ACNGT 6 cut(s) 191, 2002, 2024, 2146, 2232, 2449
TaiI ACGT 2 cut(s) 2064, 2332
TaqI TCGA 4 cut(s) 224, 688, 1800, 2349
TatI WGTACW 4 cut(s) 191, 1094, 1256, 1406
TfiI GAWTC 6 cut(s) 7, 933, 1336, 1658, 2687, 2788
Tru1I TTAA 7 cut(s) 276, 821, 1170, 1238, 1461, 2280, 2391
Tru9I TTAA 7 cut(s) 276, 821, 1170, 1238, 1461, 2280, 2391
TscAI CASTG 9 cut(s) 489, 597, 604, 994, 1039, 1444, 1981, 2851, 2865
TseFI GTSAC 1 cut(s) 1838
TseI GCWGC 8 cut(s) 141, 242, 251, 950, 1589, 2101, 2201, 2516
Tsp45I GTSAC 1 cut(s) 1838
TspGWI ACGGA 1 cut(s) 766
TspRI CASTG 9 cut(s) 489, 597, 604, 994, 1039, 1444, 1981, 2851, 2865
Van91I CCANNNNNTGG 1 cut(s) 2577
VpaK11BI GGWCC 2 cut(s) 1286, 2094
XapI RAATTY 5 cut(s) 781, 1110, 1240, 1391, 2586
XbaI TCTAGA 1 cut(s) 256
XceI RCATGY 3 cut(s) 22, 2765, 2780
XmnI GAANNNNTTC 2 cut(s) 1730, 1821
XspI CTAG 5 cut(s) 195, 257, 648, 962, 1920
ZrmI AGTACT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.