RchiOBHm_Chr3g0480811

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000051
Physical Location & Seq
Reverse (-)
26667021 .. 26670944
3924 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44582

Sequence Viewer

Length: 2625 bp
ATGATTGGTATTTTTTCTGTTAGATCTGTAGACTTGATAAAGAATTTAGAAGTGCAAGCCATCATAATAGGGCCGGAATCATCAATGCAAGCAAAGTATGCAATGAATCTTGGAGATAACGCTCAGGTGCCCATAATATCATTTTCAACAACAAGCTCTTCCATAACTACTTTCCCAACTTCGTATTTTTTCCGAGTTGCACAGAATGACTCATCTCAAGTGAAAGTCATAAGATCCATCATCCAAAACTTTGGATGGAGTGAAGCAGTACCCATCTACGTTGATTATGAGTTTGGGGAGGGAGTAATACCTTATCTGACTAATGCTTTGCAAGAGGTAGATATTCGTATCCCTTACTGGTGTGTCATTCCTTCAATGGCCAGTGATGACCAAATTGTTGCAAAGCTTCACCAGTTGATGACAATGCAAACAAGGGTATTCATAGTACACATGTTACCCTCTCTTGGCTCTCGGCTATTTGCAATCGCTAAGGAGATGGGTATGATGGGTGAAGGTTATGCTTGGATAACGACAGACAGGATGACAAACTTTTTTGGTTCCATAAAATCTTCTGTCACAGATAACATGCTAGGTGTGATAGGTTTGAAATCTTATGTTCCGAATACAGAAGAGCTTGAACATTTCAAACTTAGATGGCAAATGAAATTCCAAAAGGATAATCCGACAATCCTTAATGTTAAATTGGATGTTTTTGGATTGTGGGCTTATGATGCTGTTTGGGCATTGGCCATGGCAGTTGAAAAGGTTAGGGCTGCAAACTTCAATTTCCAAAAGTTGAACATTTCCGACCATTCCACTAATATTTTAGAAAGATTAGGTGTCTCACAAAGTGATCCTGAACTAGTACAAGCACTTTCAAGTACAGTATTTAGAGGCCTTTCAGGAAACTTTAGTCTTCTTTATGGGCAACTGCGGTCAACAAATTTTCAGATAGTTAATGTAAATGGTAATGGAGAAAGAGAGATTGGATACTGGACACTCCAAGAGGGACTTGTAAGAAACTTAAATTCAACTAGCAGAAGCACAAACACAAGCAGATTCTCTTTTATGGATGCCAATGCGTTTGGACCAATTATTTGGCCTGGAGACACGATCACCACTCCCAAAGGTTGGGAGATTCCTAAACATGGGAATTGGATGAGAGTTCTAGTTCCGGTGAATCATGGGTTTCAAGAGTTCGTACGTGTGAGCCGTGCTCCTGCTACCACTTTCAGTGGATACTGCATAGATGTATTTACAGCAGTGGTAGAAAGATTACCATATGAAGTTTCTTACGAGTTTATCCCCTTTGAAAGGCCTAATGGTGACCCAGCTGGCAGCTATGATGATTTGATCTTTCAAGTTTTTCGTGGGAATTATGATGCTGCAGTGGGAGATCTATCAAATAGACCAAACAGGTCCTTGTATGTTGACTTTACATTGCCTTACACTGATACTGGGATATCAATGATTTTGCCTGTCAAGCGCAGCAAAAGTAGTAAAAATGCATGGGTGTTCTTGAAGCCTTTGACCTGGGAGGTGTGGTTATTAAGTGTTTGTTTTTTTATATTCATGGGTTTTGTCGTCTGGGTTCTGGAGCATCGGATAAACAAAGGCTTTCGCGGGCCTCTGCAAAACCAAATTGGCACAAGTTTGTGGTTCTCCTTCTCAACCATGGTTTTCGCACACGGGGATCAACTAGTGAGCAACTTAGCTAGGTTTGTAGTGATCGTGTGGTGCTTTGTTGTCCTCGTACTGACTCAAAGTTATACCGCCAGTTTATCATCACTTTTAACAGTTCAACAGCTGCAACCAACGGATGTAAGCCTTCTCCTGAAGAATGGTGACATTGTTGGCTTACAAGAAGATTCTTTTGTCCAGGGGATTTTGGAACAAGTAGGATTTTATCCAGACAAGCTTAGGACCTATAATTCCATAGAAAGGTTGCATGAACTGTTCGAACAAGGGAGTATATCTGCTGCATTTGATGAAGCCCCTTATATGAAGGTTTTTGTTTCAACTTATTGCTCAAAATATACCGTGGTTGAGACGGCATTTTCTAAAACTGACAATGGTTTTTCCTTTGTCTTCCGAAAGGGTTCGCATCTCACACGTGATGTCTCAGGAGTGATCACTAATCTGCAAAAGGAAGATCAAATTAGAACAATCGAGGACAAGTGTTTCGGGAAGCAAGCAATTTGTATGGGCCCCAACATCACGGGCTCTTCCAACAGGCCTCTTACTCTTAGCCTTGATAGCTTCTGGGGCCTCTTCCTGATTGCTTGGCTTTCTTCATCATTAGCTCTCCTCATATTTGTAGCTATGTTCCTCTATCAGCATCGGCACATTTTGATATGCTCTGATCCAAACATCTCATTTTGGAGAAGAATTTGCATCCTACTCCAAATGTACGACCAAGAGGACATCAGTTCCCATACTATTACGCTGCAAGAAAGTGTTCATAGTCTGGTTGCAGTGGAATCCTCAGCAAGCTCCCCCAGGCCATTATCCAGTCACTCAAGCCAGACCGCATCAAACATTATTGTTTTGGAAATGCAGGAAAGATTGTCTACTAGGCTCGGTGATCTGAATCCTAATGGTCAAACAACTCAAGAGATTGCCTAG

Protein Analysis

874

Amino Acids

98.47

Weight (kDa)

5.9

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 9 - 324 1.4e-64 Receptor family ligand binding region
Peripla_BP_6 PF13458 13 - 257 3.6e-08 Periplasmic binding protein
Lig_chan-Glu_bd PF10613 409 - 493 6e-09 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 409 - 730 8.5e-08 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 511 - 762 4.9e-33 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24710 AT2G24710 AT2G24710 AT2G24720 AT2G29100 AT2G29100 AT2G29110 AT2G29110 AT2G29120 AT5G27100
fragaria_vesca FvH4_2g11260 FvH4_2g11280 FvH4_2g11310 FvH4_2g11310 FvH4_2g11331 FvH4_2g11331 FvH4_2g11340 FvH4_2g11340 FvH4_2g11340 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g11350 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31980 FvH4_2g31990 FvH4_2g37140 FvH4_2g37150 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37160 FvH4_2g37161 FvH4_2g37161 FvH4_2g37163 FvH4_2g37163 FvH4_2g37170 FvH4_2g37181 FvH4_2g37191 FvH4_3g22781 FvH4_3g22781 FvH4_3g22781 FvH4_3g22790 FvH4_3g22790 FvH4_3g22810 FvH4_5g13460 FvH4_5g34751 FvH4_7g09130 FvH4_7g27991 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010 FvH4_7g28010
malus_domestica MD08G1047600.v1.1 MD08G1047700.v1.1 MD08G1048100.v1.1 MD08G1048200.v1.1 MD08G1048300.v1.1 MD08G1048500.v1.1 MD08G1048600.v1.1 MD08G1048700.v1.1 MD08G1048900.v1.1 MD08G1049000.v1.1 MD14G1064700.v1.1 MD15G1045900.v1.1 MD15G1046000.v1.1 MD15G1046200.v1.1 MD15G1046400.v1.1 MD15G1059200.v1.1 MD15G1059500.v1.1 MD15G1059600.v1.1 MD15G1059700.v1.1
prunus_persica Prupe.1G399500_v2.0.a1 Prupe.1G399600_v2.0.a1 Prupe.1G399700_v2.0.a1 Prupe.1G399800_v2.0.a1 Prupe.1G399900_v2.0.a1 Prupe.1G400000_v2.0.a1 Prupe.1G400300_v2.0.a1 Prupe.1G400400_v2.0.a1 Prupe.1G400500_v2.0.a1 Prupe.1G400600_v2.0.a1 Prupe.1G400700_v2.0.a1 Prupe.1G412700_v2.0.a1 Prupe.1G412800_v2.0.a1
pyrus_communis pycom02g01350 pycom08g04020 pycom08g04040 pycom08g04080 pycom08g04090 pycom08g04100 pycom14g05240 pycom15g04300 pycom15g04310 pycom15g04330 pycom15g04340 pycom15g04380 pycom15g05560 pycom15g05570 pycom15g05580 pycom15g05600
rosa_chinensis RchiOBHm_Chr1g0345071 RchiOBHm_Chr1g0345081 RchiOBHm_Chr1g0345171 RchiOBHm_Chr3g0480811 RchiOBHm_Chr3g0492581 RchiOBHm_Chr5g0020871 RchiOBHm_Chr5g0039801 RchiOBHm_Chr5g0039811 RchiOBHm_Chr5g0039861 RchiOBHm_Chr5g0039911 RchiOBHm_Chr5g0039941 RchiOBHm_Chr5g0039951 RchiOBHm_Chr5g0040011 RchiOBHm_Chr6g0269601 RchiOBHm_Chr6g0269621 RchiOBHm_Chr6g0269631 RchiOBHm_Chr6g0269641 RchiOBHm_Chr6g0269671 RchiOBHm_Chr6g0269681 RchiOBHm_Chr6g0269731 RchiOBHm_Chr6g0269751 RchiOBHm_Chr6g0269761 RchiOBHm_Chr6g0269771 RchiOBHm_Chr6g0269781 RchiOBHm_Chr6g0269861 RchiOBHm_Chr6g0269871 RchiOBHm_Chr6g0269881 RchiOBHm_Chr6g0269911 RchiOBHm_Chr6g0269921 RchiOBHm_Chr6g0269941 RchiOBHm_Chr6g0269951 RchiOBHm_Chr6g0304511 RchiOBHm_Chr6g0304621 RchiOBHm_Chr6g0304641 RchiOBHm_Chr6g0304651 RchiOBHm_Chr6g0304661 RchiOBHm_Chr6g0310781 RchiOBHm_Chr6g0310801 RchiOBHm_Chr6g0310811 RchiOBHm_Chr6g0310821 RchiOBHm_Chr6g0310831 RchiOBHm_Chr7g0183621 RchiOBHm_Chr7g0183651
rosa_laevigata RLG00000005035 RLG00000005036 RLG00000010458 RLG00000010459 RLG00000010460 RLG00000010961 RLG00000010962 RLG00000010963 RLG00000010964 RLG00000010966 RLG00000010970 RLG00000013824 RLG00000013825 RLG00000013826 RLG00000013828 RLG00000013829 RLG00000013831 RLG00000013833 RLG00000013835 RLG00000013837 RLG00000013840 RLG00000013842 RLG00000013844 RLG00000022758 RLG00000023449 RLG00000026705 RLG00000028849 RLG00000028853 RLG00000028854 RLG00000032569 RLG00000033944 RLG00000033945 RLG00000033947 RLG00000033949 RLG00000033951 RLG00000034239
rosa_multiflora Rmu_co8287891.1_g000001 Rmu_co8428745.1_g000001 Rmu_co8521767.1_g000002 Rmu_sc0000103.1_g000022 Rmu_sc0000103.1_g000031 Rmu_sc0000103.1_g000041 Rmu_sc0000103.1_g000053 Rmu_sc0000247.1_g000028 Rmu_sc0000247.1_g000051 Rmu_sc0000893.1_g000003 Rmu_sc0000893.1_g000017 Rmu_sc0001143.1_g000008 Rmu_sc0001143.1_g000019 Rmu_sc0001143.1_g000023 Rmu_sc0001975.1_g000003 Rmu_sc0001975.1_g000004 Rmu_sc0001975.1_g000005 Rmu_sc0001975.1_g000012 Rmu_sc0002151.1_g000006 Rmu_sc0003610.1_g000021 Rmu_sc0003682.1_g000001 Rmu_sc0003863.1_g000004 Rmu_sc0003863.1_g000007 Rmu_sc0003863.1_g000013 Rmu_sc0004196.1_g000005 Rmu_sc0004515.1_g000013 Rmu_sc0004515.1_g000024 Rmu_sc0004688.1_g000001 Rmu_sc0004688.1_g000003 Rmu_sc0004688.1_g000004 Rmu_sc0004748.1_g000006 Rmu_sc0004838.1_g000016 Rmu_sc0004838.1_g000017 Rmu_sc0005532.1_g000006 Rmu_sc0005532.1_g000011 Rmu_sc0005950.1_g000004 Rmu_sc0006576.1_g000005 Rmu_sc0006576.1_g000021 Rmu_sc0008520.1_g000002 Rmu_sc0008520.1_g000004 Rmu_sc0009130.1_g000002 Rmu_sc0018719.1_g000001 Rmu_sc0027713.1_g000005 Rmu_sc0040804.1_g000001 Rmu_ssc0000218.1_g000059 Rmu_ssc0000218.1_g000062 Rmu_ssc0000218.1_g000068 Rmu_ssc0000395.1_g000008 Rmu_ssc0000395.1_g000009 Rmu_ssc0000395.1_g000010 Rmu_ssc0000395.1_g000011 Rmu_ssc0000395.1_g000013 Rmu_ssc0000395.1_g000014
rosa_roxburghii Rroxscaffold_1G00041270 Rroxscaffold_1G00041290 Rroxscaffold_1G00041300 Rroxscaffold_1G00041360 Rroxscaffold_1G00041380 Rroxscaffold_1G00057500 Rroxscaffold_3G00270350 Rroxscaffold_4G00283160 Rroxscaffold_4G00309210 Rroxscaffold_4G00309240 Rroxscaffold_4G00309260 Rroxscaffold_4G00309290 Rroxscaffold_4G00309360 Rroxscaffold_6G00392210 Rroxscaffold_6G00401030 Rroxscaffold_7G00158260 Rroxscaffold_7G00163620 Rroxscaffold_7G00163630 Rroxscaffold_7G00163650 Rroxscaffold_7G00163680 Rroxscaffold_7G00198270 Rroxscaffold_7G00198280 Rroxscaffold_7G00198330 Rroxscaffold_7G00198340 Rroxscaffold_7G00198360 Rroxscaffold_7G00198390 Rroxscaffold_7G00198410 Rroxscaffold_7G00198450 Rroxscaffold_7G00198460 Rroxscaffold_7G00198490 Rroxscaffold_7G00198500 Rroxscaffold_7G00198510 Rroxscaffold_7G00198530 Rroxscaffold_7G00198550 Rroxscaffold_7G00198600
rosa_rugosa Rorug01G0175900 Rorug01G0176200 Rorug03G0186700 Rorug03G0254500 Rorug05G0182700 Rorug05G0183000 Rorug05G0183000 Rorug06G0049000 Rorug06G0049500 Rorug06G0049600.1 Rorug06G0049700 Rorug06G0050000 Rorug06G0050000 Rorug06G0050100 Rorug06G0050300.1 Rorug06G0050400 Rorug06G0051100 Rorug06G0051200 Rorug06G0051300 Rorug06G0051500 Rorug06G0051600 Rorug06G0051700 Rorug06G0332600 Rorug06G0332700 Rorug06G0332700 Rorug06G0383100 Rorug06G0383200 Rorug06G0383300 Rorug06G0383400 Rorug06G0459100 Rorug06G0459200
rosa_samantha Rh1BG160400 Rh1BG160500 Rh1BG160700 Rh3DG263900 Rh3DG338400 Rh5CG164400 Rh5CG164500 Rh5CG305500 Rh5CG305900 Rh5CG306000 Rh5DG280800 Rh5DG281300 Rh6AG445300 Rh6AG497000 Rh6BG174700 Rh6BG457200 Rh6CG168900 Rh6CG169800 Rh6CG512700 Rh6CG512900 Rh6DG034100 Rh6DG160500 Rh6DG160800 Rh6DG160900 Rh6DG161100 Rh6DG161400 Rh6DG161800 Rh6DG162000 Rh6DG162100 Rh6DG162200 Rh6DG444800 Rh6DG445000 Rh6DG445100 Rh6DG445200 Rh6DG445300 Rh6DG499100 Rh6DG499200 Rh6DG499300 Rh6DG499400 Rh6DG499500 Rh6DG499700 Rh7BG059800 Rh7BG060000 Rh7BG258500 Rh7BG258700
rosa_wichuraiana Rw0G006180 Rw0G007950 Rw0G018490 Rw0G022090 Rw1G015970 Rw1G015990 Rw3G021330 Rw5G025120 Rw5G025140 Rw5G025150 Rw5G025160 Rw5G025180 Rw5G025830 Rw5G025850 Rw6G014550 Rw6G014570 Rw6G014580 Rw6G014600 Rw6G014620 Rw6G014650 Rw6G014670 Rw6G038650 Rw6G038670 Rw6G038680 Rw6G038700 Rw6G038710 Rw6G038720 Rw6G043280 Rw6G043290 Rw7G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 127
AccB7I CCANNNNNTGG 1 cut(s) 1131
AccI GTMKAC 2 cut(s) 30, 2570
AccII CGCG 1 cut(s) 1623
AciI CCGC 4 cut(s) 934, 1623, 1773, 2529
AclWI GGATC 4 cut(s) 228, 848, 1701, 2357
AcoI YGGCCR 2 cut(s) 378, 747
AcsI RAATTY 5 cut(s) 43, 665, 943, 1027, 2388
AcuI CTGAAG 1 cut(s) 1856
AcvI CACGTG 1 cut(s) 2114
AdeI CACNNNGTG 1 cut(s) 851
AfaI GTAC 7 cut(s) 270, 447, 867, 883, 1203, 1755, 2411
AfiI CCNNNNNNNGG 5 cut(s) 464, 1131, 1148, 1314, 1941
AflIII ACRYGT 3 cut(s) 450, 1204, 2111
AhlI ACTAGT 2 cut(s) 862, 1699
AjnI CCWGG 4 cut(s) 1102, 1532, 1878, 2498
AjuI GAANNNNNNNTTGG 2 cut(s) 969, 1001
Alw21I GWGCWC 1 cut(s) 1219
Alw26I GTCTC 4 cut(s) 847, 1101, 2042, 2125
AlwI GGATC 4 cut(s) 228, 848, 1701, 2357
ApaI GGGCCC 1 cut(s) 2210
ApeKI GCWGC 7 cut(s) 773, 1338, 1385, 1488, 1807, 1979, 2446
ApoI RAATTY 5 cut(s) 43, 665, 943, 1027, 2388
ArsI GACNNNNNNTTYG 6 cut(s) 310, 342, 1080, 1112, 2165, 2197
Asp700I GAANNNNTTC 2 cut(s) 2098, 2457
AspLEI GCGC 1 cut(s) 1488
AspS9I GGNCC 8 cut(s) 71, 1088, 1419, 1625, 1923, 2206, 2207, 2266
AsuHPI GGTGA 7 cut(s) 401, 521, 1108, 1189, 1337, 1856, 2594
AsuII TTCGAA 1 cut(s) 1959
AvaII GGWCC 3 cut(s) 1088, 1419, 1923
BaeGI GKGCMC 2 cut(s) 132, 2210
BalI TGGCCA 2 cut(s) 380, 749
BanI GGYRCC 1 cut(s) 127
BanII GRGCYC 2 cut(s) 2210, 2225
BbrPI CACGTG 1 cut(s) 2114
BbsI GAAGAC 2 cut(s) 908, 2080
Bbv12I GWGCWC 1 cut(s) 1219
BbvCI CCTCAGC 1 cut(s) 2485
BbvI GCAGC 7 cut(s) 760, 1350, 1372, 1500, 1794, 1966, 2433
BccI CCATC 7 cut(s) 68, 245, 249, 281, 490, 499, 648
BceAI ACGGC 2 cut(s) 1197, 2067
BciT130I CCWGG 4 cut(s) 1104, 1534, 1880, 2500
BciVI GTATCC 3 cut(s) 359, 983, 1232
BclI TGATCA 1 cut(s) 2130
BcoDI GTCTC 4 cut(s) 847, 1101, 2042, 2125
BcuI ACTAGT 2 cut(s) 862, 1699
BfaI CTAG 8 cut(s) 590, 863, 1035, 1169, 1700, 1716, 2574, 2623
BfmI CTRYAG 2 cut(s) 27, 1386
BfuI GTATCC 3 cut(s) 359, 983, 1232
BglII AGATCT 2 cut(s) 23, 1396
BisI GCNGC 7 cut(s) 774, 1339, 1386, 1489, 1808, 1980, 2447
BlsI GCNGC 7 cut(s) 775, 1340, 1387, 1490, 1809, 1981, 2448
Bme1390I CCNGG 4 cut(s) 1104, 1534, 1880, 2500
Bme18I GGWCC 3 cut(s) 1088, 1419, 1923
BmgT120I GGNCC 8 cut(s) 71, 1088, 1419, 1625, 1923, 2206, 2207, 2266
BmiI GGNNCC 5 cut(s) 129, 559, 2208, 2209, 2267
BmrFI CCNGG 4 cut(s) 1104, 1534, 1880, 2500
BmrI ACTGGG 1 cut(s) 1467
BmsI GCATC 8 cut(s) 721, 1063, 1372, 1609, 2113, 2347, 2403, 2540
BmuI ACTGGG 1 cut(s) 1467
BpiI GAAGAC 2 cut(s) 908, 2080
BplI GAGNNNNNCTC 2 cut(s) 1201, 1233
BpmI CTGGAG 2 cut(s) 1125, 1616
Bpu10I CCTNAGC 4 cut(s) 123, 489, 1919, 2485
Bpu14I TTCGAA 1 cut(s) 1959
BpuEI CTTGAG 3 cut(s) 201, 2503, 2595
BsaAI YACGTR 2 cut(s) 1205, 2114
BsaBI GATNNNNATC 1 cut(s) 2589
BsaJI CCNNGG 6 cut(s) 750, 1533, 1674, 1879, 2040, 2498
BsaWI WCCGGW 1 cut(s) 1174
BsaXI ACNNNNNCTCC 2 cut(s) 1529, 1559
Bsc4I CCNNNNNNNGG 5 cut(s) 464, 1131, 1148, 1314, 1941
Bse1I ACTGG 7 cut(s) 362, 381, 412, 998, 1462, 1776, 2511
Bse3DI GCAATG 2 cut(s) 108, 1439
Bse8I GATNNNNATC 1 cut(s) 2589
BseBI CCWGG 4 cut(s) 1104, 1534, 1880, 2500
BseDI CCNNGG 6 cut(s) 750, 1533, 1674, 1879, 2040, 2498
BseGI GGATG 8 cut(s) 240, 260, 546, 712, 1078, 1164, 1825, 2394
BseJI GATNNNNATC 1 cut(s) 2589
BseLI CCNNNNNNNGG 5 cut(s) 464, 1131, 1148, 1314, 1941
BseMI GCAATG 2 cut(s) 108, 1439
BseMII CTCAG 3 cut(s) 137, 2136, 2499
BseNI ACTGG 7 cut(s) 362, 381, 412, 998, 1462, 1776, 2511
BseRI GAGGAG 1 cut(s) 2297
BseSI GKGCMC 2 cut(s) 132, 2210
BseXI GCAGC 7 cut(s) 760, 1350, 1372, 1500, 1794, 1966, 2433
BseYI CCCAGC 1 cut(s) 1330
Bsh1236I CGCG 1 cut(s) 1623
BshNI GGYRCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 1219
BsiSI CCGG 2 cut(s) 74, 1175
BsiWI CGTACG 1 cut(s) 1201
BslFI GGGAC 1 cut(s) 1023
BslI CCNNNNNNNGG 5 cut(s) 464, 1131, 1148, 1314, 1941
BsmAI GTCTC 4 cut(s) 847, 1101, 2042, 2125
BsmBI CGTCTC 1 cut(s) 2042
BsmFI GGGAC 1 cut(s) 1023
Bsp119I TTCGAA 1 cut(s) 1959
Bsp120I GGGCCC 1 cut(s) 2206
Bsp1286I GDGCHC 4 cut(s) 132, 1219, 2210, 2225
Bsp19I CCATGG 2 cut(s) 750, 1674
BspACI CCGC 4 cut(s) 934, 1623, 1773, 2529
BspCNI CTCAG 3 cut(s) 136, 2135, 2498
BspFNI CGCG 1 cut(s) 1623
BspLI GGNNCC 5 cut(s) 129, 559, 2208, 2209, 2267
BspMAI CTGCAG 1 cut(s) 1390
BspPI GGATC 4 cut(s) 228, 848, 1701, 2357
BspQI GCTCTTC 3 cut(s) 163, 624, 2230
BspT104I TTCGAA 1 cut(s) 1959
BspT107I GGYRCC 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 108, 1439
BsrI ACTGG 7 cut(s) 362, 381, 412, 998, 1462, 1776, 2511
BssECI CCNNGG 6 cut(s) 750, 1533, 1674, 1879, 2040, 2498
BssT1I CCWWGG 2 cut(s) 750, 1674
Bst2UI CCWGG 4 cut(s) 1104, 1534, 1880, 2500
Bst4CI ACNGT 4 cut(s) 886, 1798, 1956, 2041
Bst6I CTCTTC 4 cut(s) 163, 624, 2230, 2276
BstAPI GCANNNNNTGC 1 cut(s) 98
BstBAI YACGTR 2 cut(s) 1205, 2114
BstBI TTCGAA 1 cut(s) 1959
BstC8I GCNNGC 6 cut(s) 57, 90, 1336, 1625, 2193, 2491
BstDEI CTNAG 8 cut(s) 123, 489, 650, 1711, 1919, 2122, 2246, 2485
BstDSI CCRYGG 3 cut(s) 750, 1674, 2040
BstEII GGTNACC 1 cut(s) 1325
BstENI CCTNNNNNAGG 1 cut(s) 1312
BstF5I GGATG 8 cut(s) 240, 260, 546, 712, 1078, 1164, 1825, 2394
BstFNI CGCG 1 cut(s) 1623
BstHHI GCGC 1 cut(s) 1488
BstMAI GTCTC 4 cut(s) 847, 1101, 2042, 2125
BstMWI GCNNNNNNNGC 6 cut(s) 98, 731, 740, 1483, 2256, 2265
BstNI CCWGG 4 cut(s) 1104, 1534, 1880, 2500
BstNSI RCATGY 2 cut(s) 454, 589
BstPI GGTNACC 1 cut(s) 1325
BstSCI CCNGG 4 cut(s) 1102, 1532, 1878, 2498
BstSFI CTRYAG 2 cut(s) 27, 1386
BstSLI GKGCMC 2 cut(s) 132, 2210
BstUI CGCG 1 cut(s) 1623
BstV1I GCAGC 7 cut(s) 760, 1350, 1372, 1500, 1794, 1966, 2433
BstV2I GAAGAC 2 cut(s) 908, 2080
BstX2I RGATCY 3 cut(s) 23, 233, 1396
BstXI CCANNNNNNTGG 2 cut(s) 251, 1098
BstYI RGATCY 3 cut(s) 23, 233, 1396
BsuI GTATCC 3 cut(s) 359, 983, 1232
BtgI CCRYGG 3 cut(s) 750, 1674, 2040
BtsCI GGATG 8 cut(s) 240, 260, 546, 712, 1078, 1164, 1825, 2394
BtsI GCAGTG 3 cut(s) 1269, 1395, 2481
BtsIMutI CAGTG 6 cut(s) 388, 1240, 1269, 1395, 1449, 2481
Cac8I GCNNGC 6 cut(s) 57, 90, 1336, 1625, 2193, 2491
CfoI GCGC 1 cut(s) 1488
Cfr13I GGNCC 8 cut(s) 71, 1088, 1419, 1625, 1923, 2206, 2207, 2266
Csp6I GTAC 7 cut(s) 269, 446, 866, 882, 1202, 1754, 2410
CviAII CATG 9 cut(s) 451, 586, 751, 1148, 1184, 1509, 1573, 1675, 1949
CviQI GTAC 7 cut(s) 269, 446, 866, 882, 1202, 1754, 2410
DdeI CTNAG 8 cut(s) 123, 489, 650, 1711, 1919, 2122, 2246, 2485
DraIII CACNNNGTG 1 cut(s) 851
EaeI YGGCCR 2 cut(s) 378, 747
Eam1104I CTCTTC 4 cut(s) 163, 624, 2230, 2276
EarI CTCTTC 4 cut(s) 163, 624, 2230, 2276
Eco130I CCWWGG 2 cut(s) 750, 1674
Eco147I AGGCCT 3 cut(s) 897, 1318, 2236
Eco24I GRGCYC 2 cut(s) 2210, 2225
Eco32I GATATC 1 cut(s) 1464
Eco47I GGWCC 3 cut(s) 1088, 1419, 1923
Eco57I CTGAAG 1 cut(s) 1856
Eco72I CACGTG 1 cut(s) 2114
Eco91I GGTNACC 1 cut(s) 1325
EcoNI CCTNNNNNAGG 1 cut(s) 1312
EcoO109I RGGNCCY 4 cut(s) 1419, 1923, 2207, 2266
EcoO65I GGTNACC 1 cut(s) 1325
EcoRII CCWGG 4 cut(s) 1102, 1532, 1878, 2498
EcoRV GATATC 1 cut(s) 1464
EcoT14I CCWWGG 2 cut(s) 750, 1674
EcoT22I ATGCAT 1 cut(s) 1510
EcoT38I GRGCYC 2 cut(s) 2210, 2225
ErhI CCWWGG 2 cut(s) 750, 1674
Esp3I CGTCTC 1 cut(s) 2042
FaeI CATG 9 cut(s) 454, 589, 754, 1151, 1187, 1512, 1576, 1678, 1952
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 1 cut(s) 1023
FatI CATG 9 cut(s) 450, 585, 750, 1147, 1183, 1508, 1572, 1674, 1948
FauI CCCGC 1 cut(s) 1616
FauNDI CATATG 1 cut(s) 1282
FbaI TGATCA 1 cut(s) 2130
FblI GTMKAC 2 cut(s) 30, 2570
Fnu4HI GCNGC 7 cut(s) 774, 1339, 1386, 1489, 1808, 1980, 2447
FokI GGATG 8 cut(s) 227, 267, 553, 719, 1085, 1171, 1832, 2381
FriOI GRGCYC 2 cut(s) 2210, 2225
Fsp4HI GCNGC 7 cut(s) 774, 1339, 1386, 1489, 1808, 1980, 2447
FspBI CTAG 8 cut(s) 590, 863, 1035, 1169, 1700, 1716, 2574, 2623
GlaI GCGC 1 cut(s) 1487
GluI GCNGC 7 cut(s) 774, 1339, 1386, 1489, 1808, 1980, 2447
GsaI CCCAGC 1 cut(s) 1334
GsuI CTGGAG 2 cut(s) 1125, 1616
HapII CCGG 2 cut(s) 74, 1175
HhaI GCGC 1 cut(s) 1488
Hin1II CATG 9 cut(s) 454, 589, 754, 1151, 1187, 1512, 1576, 1678, 1952
Hin6I GCGC 1 cut(s) 1486
HinP1I GCGC 1 cut(s) 1486
HincII GTYRAC 2 cut(s) 939, 1432
HindII GTYRAC 2 cut(s) 939, 1432
HindIII AAGCTT 2 cut(s) 404, 1916
HpaII CCGG 2 cut(s) 74, 1175
HphI GGTGA 7 cut(s) 401, 521, 1108, 1189, 1337, 1856, 2594
Hpy166II GTNNAC 5 cut(s) 31, 448, 939, 1432, 2571
Hpy8I GTNNAC 5 cut(s) 31, 448, 939, 1432, 2571
HpyAV CCTTC 5 cut(s) 381, 506, 1675, 1838, 1999
HpyCH4III ACNGT 4 cut(s) 886, 1798, 1956, 2041
HpyCH4IV ACGT 3 cut(s) 279, 1204, 2113
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 731, 740, 1483, 2256, 2265
HpyF3I CTNAG 8 cut(s) 123, 489, 650, 1711, 1919, 2122, 2246, 2485
HpySE526I ACGT 3 cut(s) 279, 1204, 2113
Hsp92II CATG 9 cut(s) 454, 589, 754, 1151, 1187, 1512, 1576, 1678, 1952
HspAI GCGC 1 cut(s) 1486
Ksp22I TGATCA 1 cut(s) 2130
LguI GCTCTTC 3 cut(s) 163, 624, 2230
LmnI GCTCC 3 cut(s) 1222, 1597, 2498
Lsp1109I GCAGC 7 cut(s) 760, 1350, 1372, 1500, 1794, 1966, 2433
LweI GCATC 8 cut(s) 721, 1063, 1372, 1609, 2113, 2347, 2403, 2540
MaeI CTAG 8 cut(s) 590, 863, 1035, 1169, 1700, 1716, 2574, 2623
MaeII ACGT 3 cut(s) 279, 1204, 2113
MaeIII GTNAC 5 cut(s) 453, 574, 1325, 1844, 2513
MflI RGATCY 3 cut(s) 23, 233, 1396
MhlI GDGCHC 4 cut(s) 132, 1219, 2210, 2225
MlsI TGGCCA 2 cut(s) 380, 749
MluNI TGGCCA 2 cut(s) 380, 749
MlyI GAGTC 2 cut(s) 203, 1753
MmeI TCCRAC 3 cut(s) 707, 831, 2253
Mox20I TGGCCA 2 cut(s) 380, 749
Mph1103I ATGCAT 1 cut(s) 1510
MroXI GAANNNNTTC 2 cut(s) 2098, 2457
MscI TGGCCA 2 cut(s) 380, 749
MseI TTAA 6 cut(s) 693, 699, 957, 1025, 1550, 1793
Msp20I TGGCCA 2 cut(s) 380, 749
MspA1I CMGCKG 2 cut(s) 1334, 1807
MspI CCGG 2 cut(s) 74, 1175
MspR9I CCNGG 4 cut(s) 1104, 1534, 1880, 2500
MvaI CCWGG 4 cut(s) 1104, 1534, 1880, 2500
MvnI CGCG 1 cut(s) 1623
MwoI GCNNNNNNNGC 6 cut(s) 98, 731, 740, 1483, 2256, 2265
NcoI CCATGG 2 cut(s) 750, 1674
NdeI CATATG 1 cut(s) 1282
NlaIII CATG 9 cut(s) 454, 589, 754, 1151, 1187, 1512, 1576, 1678, 1952
NlaIV GGNNCC 5 cut(s) 129, 559, 2208, 2209, 2267
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 4 cut(s) 574, 1325, 1844, 2513
NsiI ATGCAT 1 cut(s) 1510
NspI RCATGY 2 cut(s) 454, 589
NspV TTCGAA 1 cut(s) 1959
PceI AGGCCT 3 cut(s) 897, 1318, 2236
PciI ACATGT 1 cut(s) 450
PciSI GCTCTTC 3 cut(s) 163, 624, 2230
PcsI WCGNNNNNNNCGW 1 cut(s) 1210
PdmI GAANNNNTTC 2 cut(s) 2098, 2457
PfeI GAWTC 8 cut(s) 77, 106, 1059, 1138, 1180, 1868, 2480, 2590
Pfl23II CGTACG 1 cut(s) 1201
PflMI CCANNNNNTGG 1 cut(s) 1131
PkrI GCNGC 7 cut(s) 775, 1340, 1387, 1490, 1809, 1981, 2448
PleI GAGTC 2 cut(s) 203, 1753
PmaCI CACGTG 1 cut(s) 2114
PmlI CACGTG 1 cut(s) 2114
PpsI GAGTC 2 cut(s) 203, 1753
Ppu21I YACGTR 2 cut(s) 1205, 2114
PpuMI RGGWCCY 2 cut(s) 1419, 1923
PscI ACATGT 1 cut(s) 450
Psp5II RGGWCCY 2 cut(s) 1419, 1923
Psp6I CCWGG 4 cut(s) 1102, 1532, 1878, 2498
PspCI CACGTG 1 cut(s) 2114
PspEI GGTNACC 1 cut(s) 1325
PspFI CCCAGC 1 cut(s) 1330
PspGI CCWGG 4 cut(s) 1102, 1532, 1878, 2498
PspLI CGTACG 1 cut(s) 1201
PspN4I GGNNCC 5 cut(s) 129, 559, 2208, 2209, 2267
PspOMI GGGCCC 1 cut(s) 2206
PspPI GGNCC 8 cut(s) 71, 1088, 1419, 1625, 1923, 2206, 2207, 2266
PspPPI RGGWCCY 2 cut(s) 1419, 1923
PstI CTGCAG 1 cut(s) 1390
PsuI RGATCY 3 cut(s) 23, 233, 1396
PvuII CAGCTG 2 cut(s) 1334, 1807
RsaI GTAC 7 cut(s) 270, 447, 867, 883, 1203, 1755, 2411
RsaNI GTAC 7 cut(s) 269, 446, 866, 882, 1202, 1754, 2410
SapI GCTCTTC 3 cut(s) 163, 624, 2230
SaqAI TTAA 6 cut(s) 693, 699, 957, 1025, 1550, 1793
SatI GCNGC 7 cut(s) 774, 1339, 1386, 1489, 1808, 1980, 2447
Sau96I GGNCC 8 cut(s) 71, 1088, 1419, 1625, 1923, 2206, 2207, 2266
SchI GAGTC 2 cut(s) 203, 1753
ScrFI CCNGG 4 cut(s) 1104, 1534, 1880, 2500
SduI GDGCHC 4 cut(s) 132, 1219, 2210, 2225
SfaNI GCATC 8 cut(s) 721, 1063, 1372, 1609, 2113, 2347, 2403, 2540
SfcI CTRYAG 2 cut(s) 27, 1386
SfuI TTCGAA 1 cut(s) 1959
SinI GGWCC 3 cut(s) 1088, 1419, 1923
SmlI CTYRAG 3 cut(s) 216, 2518, 2610
SmoI CTYRAG 3 cut(s) 216, 2518, 2610
SpeI ACTAGT 2 cut(s) 862, 1699
SseBI AGGCCT 3 cut(s) 897, 1318, 2236
SsiI CCGC 4 cut(s) 934, 1623, 1773, 2529
SspI AATATT 1 cut(s) 823
SspMI CTAG 8 cut(s) 590, 863, 1035, 1169, 1700, 1716, 2574, 2623
StuI AGGCCT 3 cut(s) 897, 1318, 2236
StyD4I CCNGG 4 cut(s) 1102, 1532, 1878, 2498
StyI CCWWGG 2 cut(s) 750, 1674
TaaI ACNGT 4 cut(s) 886, 1798, 1956, 2041
TaiI ACGT 3 cut(s) 282, 1207, 2116
TaqI TCGA 2 cut(s) 1959, 2169
TatI WGTACW 3 cut(s) 445, 865, 881
TfiI GAWTC 8 cut(s) 77, 106, 1059, 1138, 1180, 1868, 2480, 2590
Tru1I TTAA 6 cut(s) 693, 699, 957, 1025, 1550, 1793
Tru9I TTAA 6 cut(s) 693, 699, 957, 1025, 1550, 1793
TscAI CASTG 6 cut(s) 388, 1240, 1269, 1395, 1456, 2481
TseFI GTSAC 4 cut(s) 574, 1325, 1844, 2513
TseI GCWGC 7 cut(s) 773, 1338, 1385, 1488, 1807, 1979, 2446
Tsp45I GTSAC 4 cut(s) 574, 1325, 1844, 2513
TspGWI ACGGA 1 cut(s) 1832
TspRI CASTG 6 cut(s) 388, 1240, 1269, 1395, 1456, 2481
Van91I CCANNNNNTGG 1 cut(s) 1131
VpaK11BI GGWCC 3 cut(s) 1088, 1419, 1923
XagI CCTNNNNNAGG 1 cut(s) 1312
XapI RAATTY 5 cut(s) 43, 665, 943, 1027, 2388
XceI RCATGY 2 cut(s) 454, 589
XmiI GTMKAC 2 cut(s) 30, 2570
XmnI GAANNNNTTC 2 cut(s) 2098, 2457
XspI CTAG 8 cut(s) 590, 863, 1035, 1169, 1700, 1716, 2574, 2623
Zsp2I ATGCAT 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.