FvH4_1g15090

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000039
Physical Location & Seq
Forward (+)
8522837 .. 8527445
4609 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15090.t1

Sequence Viewer

Length: 2397 bp
ATGGACCTTGTTGAAGGGGGTGCTATAGGAGTACCATTTTCTGCGCTTTATGACGTCATTAAGGAAGTGGTGGTCAAGACTAAGATGTTTAAACCTCTCCTCGTGGATCTTCGTTCGACGATAGAAACTTTGAAACCACTGATCGAGGAGATGGAAAAGAGCAATAAGGTGTTGGATCATCCAGAGAATGAACTTAGAGGATTTAAAGTACACATGCAAAAGGGCGCACGGCTGATTCACAATTGCTCTGAGGTTAGGTGGTGGAATATTTGCAAAACTATTAAATACACTAACCAACTTCATGGATTAAAGAGCTCCCTTGGGAGTTTGTATGATATACTGAAAGTGCAAGAAGCAAGGGATGTGAAAAATATAGAGACAGTGATACATCGGATTTTGGACATTTTGGTACACAATAAGAACCCGGTCCATGGTTGGTGTGCAGTACCTGTAGGTGCAACATTTACAGTTGGAATGGAAATGCCTTTGAGGGTATTGAAGACCAAGCTTCTTAAAGATGAGACGGTAACAATGCTTGTGCTCACTGCTGCAGGAGGATGTGGGAAAACCACTTTAGCAATTGAGTTTTGTGAAGATTCCGAAGTCAAAGACAAATTTAAGGACAATATCTTCTTTGTCACCGTCTCAAAGAAGTCCGATGAGCTTGTAGTACGAGAACTATATCTCAGCAAAGGTTCTGAAATACCTACTTTGCCCAACGGACTTGATGTGGGTAAGTGGCTAAAGAGTTTCCTGACGGCAGTAGGAGAGAATCCTGTACTTTTTGTCCTGGATGATGTTTGGTCTGGATCGGTATCCCTTCTTCAGAATTTTGTTGATATCAAATTGCCAAATTCCAAGATTTTGGTGACATCAAGATTTAGATTTCCATTTGGTTATCGACATAGTCTAGATGCGTTGAAGGTTGAAGATGCAACAACTCTTTTCAAACATGCAGCATTCCTGGGAGATAGGAGTGATGACATTCCTGAAGATATTGTGAGAGAGATAGTAAAGCATTACAAAAGTTCTCCACTTGCCATTACAGTGGTTGGACGATCTCTTTGTCAAAAATCGGTAGAGTGTTGGCTGAGTACAGCATCTTCACTAAAAGGTGCTTCTATTCTTGATTCTGAGACTTCTTTGCTACATGGCCTTCAGAGTTGTTTAGATGCCTTAAATGAAGAAGGTGATAGCTTGAGGGAATGCTTCTTAGACCTCGGTTCATTTCCTGAAGACCAAACAATCCCTGCCGCTGCTCTCATTGATATGTGGGCGGAGTTATATCAGCTAAAAGATTTTGAGTCCATTAACAATCTCCACAAGCTCGTTGATCGAAGTCTGGCTAATCTTCGAATTACAAGAAAGGAGAAGATGCAGGCAGATGGGTACTACACTGACCACTTTGTTACTCAGCATGATTTGCTTAGACAGTTGGCTATCTACCAGCCAAAGCTAGACCCGGATTATAAAAGACGGATCATAGACAAATGGGGAGACAATCTACCCAAATGCTACACAGAACAAAAACCCATCAAGGCCCGCCTATTATCTATCTCCTCTGATGGAGTGTTCTCGACAGATTTGCACAACATTGAATTGGCAGCGGTTGAAGTTCTAGTTTTGAATTTTAACACACAGAACTATGCTTTACCAGAATTTGTGGTCAAGATGGACGAACTGAAGGTTTTAATAGTCACAAATAATGGTATCATACCTGCTGAATTGTGTAACATTCAACTGCTGGGTTCTTCAACAAATCTGAAGAAAATCAGATTAGAGCGCATTTCAATTTCTTCCATAACCAAGAATCTAATAGAGTTGAAGAGTGTGACGAAGATATCTCTATTCATGTGCAAGGTTGGTCAAGCTTTCAGCAATTGTTCCCTTCAAATCTCAGCTGCATTGCCATGTTTACAGGAAATGAACATTGACTACTGTAATGATTTGGTGGAGTTGCCTGCCGTGCTTTGTGCTCTGGTTAACCTAAAGATGCTCAGTATCACCAACTCGCATAAGCTATCTGCCTTGCCGGAAGAGATTGGAAATCTGGACAATTTAGAAATATTGAGGCTAAGGTCTTGTACAGACTTGACAATGTTGCCAAACTCAAGTACGAACCTAAAGAAGCTAAAGTTTCTTGACATGGCTGATTGCTTCAGCATTCGGGAATTGCCTGAAGACTTTGGTGGACTGAGCAGTTTAGAAAACATCAACATGAGAATGTGTTCGAGATTGAGAGAACTGCCTCCATCAGTTATGGATCTTGAGAAGTTACAGAAAGTGGTATGTGATGCAGACACAATCAATCTATGGGAACCCTTCTTACCCCATCTCCCAAACCTCCGTATGAAGCTGGTCGATGAAAACGTCAACTTGGATTGGCTACTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

799

Amino Acids

89.91

Weight (kDa)

5.95

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 2 - 126 4.8e-28 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 162 - 319 5.5e-11 NB-ARC domain
LRR_14 PF23598 657 - 717 3.1e-09 Leucine-rich repeat region
LRR_14 PF23598 707 - 769 9.4e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1470
AatII GACGTC 1 cut(s) 57
Acc36I ACCTGC 1 cut(s) 1726
AciI CCGC 4 cut(s) 1254, 1277, 1543, 1607
AclWI GGATC 5 cut(s) 114, 183, 817, 1487, 2271
AcsI RAATTY 5 cut(s) 614, 829, 853, 1627, 1658
AcuI CTGAAG 8 cut(s) 809, 1011, 1142, 1254, 1703, 1784, 2143, 2199
AcyI GRCGYC 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 431
AjnI CCWGG 2 cut(s) 789, 963
Alw21I GWGCWC 3 cut(s) 317, 543, 1978
Alw26I GTCTC 5 cut(s) 371, 515, 649, 1130, 1491
AlwI GGATC 5 cut(s) 114, 183, 817, 1487, 2271
AlwNI CAGNNNCTG 1 cut(s) 449
AoxI GGCC 2 cut(s) 1153, 1539
ApeKI GCWGC 5 cut(s) 548, 956, 1256, 1604, 1901
ApoI RAATTY 5 cut(s) 614, 829, 853, 1627, 1658
ArsI GACNNNNNNTTYG 2 cut(s) 1047, 1079
Asp700I GAANNNNTTC 1 cut(s) 2227
AspLEI GCGC 3 cut(s) 46, 227, 1785
AspS9I GGNCC 3 cut(s) 4, 427, 1540
AsuC2I CCSGG 2 cut(s) 425, 1463
AsuHPI GGTGA 4 cut(s) 631, 880, 1202, 1996
AsuII TTCGAA 1 cut(s) 1354
AvaII GGWCC 2 cut(s) 4, 427
BanII GRGCYC 1 cut(s) 317
BarI GAAGNNNNNNTAC 4 cut(s) 807, 839, 1087, 1119
BauI CACGAG 1 cut(s) 101
BbsI GAAGAC 3 cut(s) 506, 1242, 2187
Bbv12I GWGCWC 3 cut(s) 317, 543, 1978
BbvI GCAGC 5 cut(s) 535, 968, 1243, 1616, 1888
BccI CCATC 7 cut(s) 145, 1379, 1541, 1559, 1666, 2260, 2340
BceAI ACGGC 3 cut(s) 245, 774, 1949
BcgI CGANNNNNNTGC 2 cut(s) 1567, 1601
BciT130I CCWGG 2 cut(s) 791, 965
BciVI GTATCC 1 cut(s) 826
BcnI CCSGG 2 cut(s) 425, 1463
BcoDI GTCTC 5 cut(s) 371, 515, 649, 1130, 1491
BfaI CTAG 3 cut(s) 911, 1457, 1619
BfmI CTRYAG 3 cut(s) 24, 450, 549
BfuAI ACCTGC 1 cut(s) 1726
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 6 cut(s) 549, 957, 1254, 1257, 1605, 1902
BlsI GCNGC 6 cut(s) 550, 958, 1255, 1258, 1606, 1903
Bme1390I CCNGG 4 cut(s) 425, 791, 965, 1463
Bme18I GGWCC 2 cut(s) 4, 427
BmgT120I GGNCC 3 cut(s) 4, 427, 1540
BmiI GGNNCC 1 cut(s) 2319
BmrFI CCNGG 4 cut(s) 425, 791, 965, 1463
BmsI GCATC 7 cut(s) 904, 922, 1109, 1162, 1365, 1983, 2284
BpiI GAAGAC 3 cut(s) 506, 1242, 2187
Bpu10I CCTNAGC 1 cut(s) 2075
Bpu14I TTCGAA 1 cut(s) 1354
BpuEI CTTGAG 3 cut(s) 1219, 2095, 2288
BpuMI CCSGG 2 cut(s) 425, 1463
BsaBI GATNNNNATC 1 cut(s) 814
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 4 cut(s) 319, 430, 964, 1219
BsaXI ACNNNNNCTCC 2 cut(s) 2319, 2349
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse3DI GCAATG 1 cut(s) 1904
Bse8I GATNNNNATC 1 cut(s) 814
BseBI CCWGG 2 cut(s) 791, 965
BseDI CCNNGG 4 cut(s) 319, 430, 964, 1219
BseGI GGATG 4 cut(s) 178, 367, 563, 799
BseJI GATNNNNATC 1 cut(s) 814
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMI GCAATG 1 cut(s) 1904
BseMII CTCAG 8 cut(s) 240, 700, 1082, 1125, 1427, 1911, 2011, 2186
BseRI GAGGAG 3 cut(s) 89, 161, 1549
BseXI GCAGC 5 cut(s) 535, 968, 1243, 1616, 1888
BseYI CCCAGC 1 cut(s) 1744
BsgI GTGCAG 1 cut(s) 462
BshFI GGCC 2 cut(s) 1155, 1541
BsiHKAI GWGCWC 3 cut(s) 317, 543, 1978
BsiSI CCGG 3 cut(s) 425, 1463, 2033
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 5 cut(s) 371, 515, 649, 1130, 1491
BsmBI CGTCTC 2 cut(s) 515, 649
BsmI GAATGC 3 cut(s) 959, 1211, 2163
BsnI GGCC 2 cut(s) 1155, 1541
Bsp119I TTCGAA 1 cut(s) 1354
Bsp1286I GDGCHC 3 cut(s) 317, 543, 1978
Bsp1407I TGTACA 1 cut(s) 2084
Bsp143I GATC 8 cut(s) 106, 141, 175, 809, 1058, 1333, 1479, 2263
Bsp19I CCATGG 1 cut(s) 430
BspACI CCGC 4 cut(s) 1254, 1277, 1543, 1607
BspANI GGCC 2 cut(s) 1155, 1541
BspCNI CTCAG 8 cut(s) 241, 699, 1083, 1126, 1426, 1910, 2010, 2187
BspLI GGNNCC 1 cut(s) 2319
BspMAI CTGCAG 1 cut(s) 553
BspMI ACCTGC 1 cut(s) 1726
BspPI GGATC 5 cut(s) 114, 183, 817, 1487, 2271
BspT104I TTCGAA 1 cut(s) 1354
BsrDI GCAATG 1 cut(s) 1904
BsrGI TGTACA 1 cut(s) 2084
BssECI CCNNGG 4 cut(s) 319, 430, 964, 1219
BssMI GATC 8 cut(s) 106, 141, 175, 809, 1058, 1333, 1479, 2263
BssNI GRCGYC 1 cut(s) 54
BssSI CACGAG 1 cut(s) 101
BssT1I CCWWGG 2 cut(s) 319, 430
Bst2BI CACGAG 1 cut(s) 101
Bst2UI CCWGG 2 cut(s) 791, 965
Bst4CI ACNGT 7 cut(s) 382, 469, 526, 643, 1048, 1434, 1940
Bst6I CTCTTC 2 cut(s) 1820, 2031
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 1423
BstAUI TGTACA 1 cut(s) 2084
BstBI TTCGAA 1 cut(s) 1354
BstC8I GCNNGC 3 cut(s) 1380, 1543, 1962
BstDSI CCRYGG 1 cut(s) 430
BstF5I GGATG 4 cut(s) 178, 367, 563, 799
BstHHI GCGC 3 cut(s) 46, 227, 1785
BstKTI GATC 8 cut(s) 109, 144, 178, 812, 1061, 1336, 1482, 2266
BstMAI GTCTC 5 cut(s) 371, 515, 649, 1130, 1491
BstMBI GATC 8 cut(s) 106, 141, 175, 809, 1058, 1333, 1479, 2263
BstMWI GCNNNNNNNGC 2 cut(s) 1423, 1966
BstNI CCWGG 2 cut(s) 791, 965
BstNSI RCATGY 2 cut(s) 217, 956
BstSCI CCNGG 4 cut(s) 423, 789, 963, 1461
BstSFI CTRYAG 3 cut(s) 24, 450, 549
BstV1I GCAGC 5 cut(s) 535, 968, 1243, 1616, 1888
BstV2I GAAGAC 3 cut(s) 506, 1242, 2187
BstX2I RGATCY 2 cut(s) 106, 2263
BstXI CCANNNNNNTGG 3 cut(s) 302, 865, 1048
BstYI RGATCY 2 cut(s) 106, 2263
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 2 cut(s) 1155, 1541
BtgI CCRYGG 1 cut(s) 430
BtsCI GGATG 4 cut(s) 178, 367, 563, 799
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 5 cut(s) 137, 387, 543, 1053, 1395
BveI ACCTGC 1 cut(s) 1726
Cac8I GCNNGC 3 cut(s) 1380, 1543, 1962
CaiI CAGNNNCTG 1 cut(s) 449
CfoI GCGC 3 cut(s) 46, 227, 1785
Cfr13I GGNCC 3 cut(s) 4, 427, 1540
DpnI GATC 8 cut(s) 108, 143, 177, 811, 1060, 1335, 1481, 2265
DpnII GATC 8 cut(s) 106, 141, 175, 809, 1058, 1333, 1479, 2263
DraI TTTAAA 2 cut(s) 91, 205
Eam1104I CTCTTC 2 cut(s) 1820, 2031
EarI CTCTTC 2 cut(s) 1820, 2031
EciI GGCGGA 1 cut(s) 1292
Ecl136II GAGCTC 1 cut(s) 315
Eco130I CCWWGG 2 cut(s) 319, 430
Eco24I GRGCYC 1 cut(s) 317
Eco32I GATATC 2 cut(s) 841, 1842
Eco47I GGWCC 2 cut(s) 4, 427
Eco53kI GAGCTC 1 cut(s) 315
Eco57I CTGAAG 8 cut(s) 809, 1011, 1142, 1254, 1703, 1784, 2143, 2199
EcoICRI GAGCTC 1 cut(s) 315
EcoRII CCWGG 2 cut(s) 789, 963
EcoRV GATATC 2 cut(s) 841, 1842
EcoT14I CCWWGG 2 cut(s) 319, 430
EcoT38I GRGCYC 1 cut(s) 317
ErhI CCWWGG 2 cut(s) 319, 430
Esp3I CGTCTC 2 cut(s) 515, 649
FauI CCCGC 1 cut(s) 1550
Fnu4HI GCNGC 6 cut(s) 549, 957, 1254, 1257, 1605, 1902
FokI GGATG 4 cut(s) 165, 374, 570, 806
FriOI GRGCYC 1 cut(s) 317
Fsp4HI GCNGC 6 cut(s) 549, 957, 1254, 1257, 1605, 1902
FspBI CTAG 3 cut(s) 911, 1457, 1619
GlaI GCGC 3 cut(s) 45, 226, 1784
GluI GCNGC 6 cut(s) 549, 957, 1254, 1257, 1605, 1902
GsaI CCCAGC 1 cut(s) 1748
HaeIII GGCC 2 cut(s) 1155, 1541
HapII CCGG 3 cut(s) 425, 1463, 2033
HhaI GCGC 3 cut(s) 46, 227, 1785
Hin1I GRCGYC 1 cut(s) 54
Hin6I GCGC 3 cut(s) 44, 225, 1783
HinP1I GCGC 3 cut(s) 44, 225, 1783
HincII GTYRAC 2 cut(s) 1984, 2374
HindII GTYRAC 2 cut(s) 1984, 2374
HindIII AAGCTT 2 cut(s) 506, 1869
HinfI GANTC 6 cut(s) 235, 596, 772, 1130, 1304, 1810
HpaI GTTAAC 1 cut(s) 1984
HpaII CCGG 3 cut(s) 425, 1463, 2033
HphI GGTGA 4 cut(s) 631, 880, 1202, 1996
Hpy166II GTNNAC 6 cut(s) 211, 412, 1916, 1984, 2192, 2374
Hpy8I GTNNAC 6 cut(s) 211, 412, 1916, 1984, 2192, 2374
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 8 cut(s) 8, 830, 916, 1166, 1181, 1678, 1898, 2332
HpyCH4III ACNGT 7 cut(s) 382, 469, 526, 643, 1048, 1434, 1940
HpyCH4IV ACGT 2 cut(s) 54, 2370
HpyF10VI GCNNNNNNNGC 2 cut(s) 1423, 1966
HpySE526I ACGT 2 cut(s) 54, 2370
Hsp92I GRCGYC 1 cut(s) 54
HspAI GCGC 3 cut(s) 44, 225, 1783
KspAI GTTAAC 1 cut(s) 1984
Kzo9I GATC 8 cut(s) 106, 141, 175, 809, 1058, 1333, 1479, 2263
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 5 cut(s) 535, 968, 1243, 1616, 1888
LweI GCATC 7 cut(s) 904, 922, 1109, 1162, 1365, 1983, 2284
MaeI CTAG 3 cut(s) 911, 1457, 1619
MaeII ACGT 2 cut(s) 54, 2370
MaeIII GTNAC 8 cut(s) 526, 637, 868, 1408, 1696, 1730, 1831, 2274
MalI GATC 8 cut(s) 108, 143, 177, 811, 1060, 1335, 1481, 2265
MboI GATC 8 cut(s) 106, 141, 175, 809, 1058, 1333, 1479, 2263
MfeI CAATTG 3 cut(s) 241, 579, 1879
MflI RGATCY 2 cut(s) 106, 2263
MhlI GDGCHC 3 cut(s) 317, 543, 1978
MlyI GAGTC 1 cut(s) 1313
MmeI TCCRAC 3 cut(s) 153, 451, 1033
MroXI GAANNNNTTC 1 cut(s) 2227
MslI CAYNNNNRTG 5 cut(s) 1046, 1268, 1909, 2216, 2222
MspA1I CMGCKG 3 cut(s) 1256, 1607, 1901
MspI CCGG 3 cut(s) 425, 1463, 2033
MspR9I CCNGG 4 cut(s) 425, 791, 965, 1463
MssI GTTTAAAC 1 cut(s) 91
MunI CAATTG 3 cut(s) 241, 579, 1879
Mva1269I GAATGC 3 cut(s) 959, 1211, 2163
MvaI CCWGG 2 cut(s) 791, 965
MwoI GCNNNNNNNGC 2 cut(s) 1423, 1966
NciI CCSGG 2 cut(s) 425, 1463
NcoI CCATGG 1 cut(s) 430
NdeII GATC 8 cut(s) 106, 141, 175, 809, 1058, 1333, 1479, 2263
NlaIV GGNNCC 1 cut(s) 2319
NmuCI GTSAC 4 cut(s) 637, 868, 1696, 1831
NspI RCATGY 2 cut(s) 217, 956
NspV TTCGAA 1 cut(s) 1354
PcsI WCGNNNNNNNCGW 1 cut(s) 2367
PctI GAATGC 3 cut(s) 959, 1211, 2163
PdmI GAANNNNTTC 1 cut(s) 2227
PfeI GAWTC 5 cut(s) 235, 596, 772, 1130, 1810
PflFI GACNNNGTC 1 cut(s) 906
PfoI TCCNGGA 1 cut(s) 789
PkrI GCNGC 6 cut(s) 550, 958, 1255, 1258, 1606, 1903
PleI GAGTC 1 cut(s) 1312
PmeI GTTTAAAC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 1312
PsiI TTATAA 1 cut(s) 1470
Psp124BI GAGCTC 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 789, 963
PspFI CCCAGC 1 cut(s) 1744
PspGI CCWGG 2 cut(s) 789, 963
PspN4I GGNNCC 1 cut(s) 2319
PspPI GGNCC 3 cut(s) 4, 427, 1540
PsrI GAACNNNNNNTAC 4 cut(s) 1919, 1951, 2310, 2342
PstI CTGCAG 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 2 cut(s) 106, 2263
PsyI GACNNNGTC 1 cut(s) 906
PvuII CAGCTG 1 cut(s) 1901
RseI CAYNNNNRTG 5 cut(s) 1046, 1268, 1909, 2216, 2222
SacI GAGCTC 1 cut(s) 317
SatI GCNGC 6 cut(s) 549, 957, 1254, 1257, 1605, 1902
Sau3AI GATC 8 cut(s) 106, 141, 175, 809, 1058, 1333, 1479, 2263
Sau96I GGNCC 3 cut(s) 4, 427, 1540
SchI GAGTC 1 cut(s) 1313
ScrFI CCNGG 4 cut(s) 425, 791, 965, 1463
SduI GDGCHC 3 cut(s) 317, 543, 1978
SfaNI GCATC 7 cut(s) 904, 922, 1109, 1162, 1365, 1983, 2284
SfcI CTRYAG 3 cut(s) 24, 450, 549
SfuI TTCGAA 1 cut(s) 1354
SinI GGWCC 2 cut(s) 4, 427
SmiMI CAYNNNNRTG 5 cut(s) 1046, 1268, 1909, 2216, 2222
SmlI CTYRAG 3 cut(s) 1198, 2110, 2267
SmoI CTYRAG 3 cut(s) 1198, 2110, 2267
SsiI CCGC 4 cut(s) 1254, 1277, 1543, 1607
SspI AATATT 2 cut(s) 268, 2067
SspMI CTAG 3 cut(s) 911, 1457, 1619
SstI GAGCTC 1 cut(s) 317
StyD4I CCNGG 4 cut(s) 423, 789, 963, 1461
StyI CCWWGG 2 cut(s) 319, 430
TaaI ACNGT 7 cut(s) 382, 469, 526, 643, 1048, 1434, 1940
TaiI ACGT 2 cut(s) 57, 2373
TaqI TCGA 8 cut(s) 116, 144, 901, 1336, 1354, 1577, 2231, 2361
TatI WGTACW 4 cut(s) 208, 778, 1094, 2084
TauI GCSGC 1 cut(s) 1256
TfiI GAWTC 5 cut(s) 235, 596, 772, 1130, 1810
TscAI CASTG 5 cut(s) 144, 387, 550, 1053, 1402
TseFI GTSAC 4 cut(s) 637, 868, 1696, 1831
TseI GCWGC 5 cut(s) 548, 956, 1256, 1604, 1901
Tsp45I GTSAC 4 cut(s) 637, 868, 1696, 1831
TspDTI ATGAA 8 cut(s) 204, 290, 1197, 1215, 1840, 1940, 2366, 2379
TspGWI ACGGA 3 cut(s) 735, 1492, 2336
TspRI CASTG 5 cut(s) 144, 387, 550, 1053, 1402
Tth111I GACNNNGTC 1 cut(s) 906
VpaK11BI GGWCC 2 cut(s) 4, 427
XapI RAATTY 5 cut(s) 614, 829, 853, 1627, 1658
XbaI TCTAGA 1 cut(s) 910
XceI RCATGY 2 cut(s) 217, 956
XmnI GAANNNNTTC 1 cut(s) 2227
XspI CTAG 3 cut(s) 911, 1457, 1619
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.