FvH4_1g15171

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000039
Physical Location & Seq
Forward (+)
8607336 .. 8611134
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15171.t1

Sequence Viewer

Length: 2472 bp
ATGGAACTGGCTATTACCGCAGTTACCACAATTGTAGGGGCTGCTCTAGGAGCAGCGTTCCAGCAGCTCTTTGATAGTGTTATAAAGGCACACAAAAAAGCAACAAAGTTCAAATCCATCTTCAACGAGCTCAATTTGACACTCGAGTCTTATCAAGCATTTGTTAAGTATATAGAGCAAGACATCAATGTTCAACCAATGCACGGCCTAGATGCTTTTAAAGTGAAGATGGAGGAGGGCATACAGCTTGTTGAAAAGTGCTCCAATATTCATCCCCTGCGGTTGAAGATGAGTTCTTTCAAAAGTCGCAATGTGGGAAAACTTCGTGCATTGAACATCTCTCTTCAAACACTCTTCCGCATACTAAAAATGCATGTAGAAATCAACCTGACGGGCAAGGTGAGCAAGTTGGAGACGTCGGTCAATCGAATAGATCAAAACGTGCTGGCTCAAAGACAAACAGGAAATGAAATTAGAGGCTGGTGCGCAGTACTTAATCCGCCATCGTTTACTGTTGGATTGGATGAGCCTTTGAACGATTTGAAGATGAAGTTACTATATAATGATCAACTGTCAATGCTTGTGCTCACTGCTCCTGGAGGATGCGGGAAAACCACTTTGGCACAGAAGTTCTGTCAAGATAAGCAAGTCCAAGAAAAATTCAAGAAGAATATTTACTTTGTCACTGTTTCGGAAAAGCCCAACGTGGAGCGTATTGTAGAAGAAATGTGCCAGAGCACTGGATCCCAAGTTCCTGAATTCCAGAATGAAGCTAATGCGCTAGGCTGGCTGCAAGAGTTTTTGGTAAAAGCAGGACAGAATCCTCTGCTTTTGGTCTTGGATGATGTTTGGCTTGGCTCTTCAGAATCCCTGCTTGATAAGTGTCATGAATTCAAAAGGCCAAATTACAAGATTTTGGTGACATCAAGATTTCATTTCAAAAGATTTCGTTCTTCATATGAATTAAAACCACTAAATGAAAAAGATGCAATGTCCCTTTTCCGTGATACAACATCGTTGCAAGATAGCGAGTATGATATCCCTACAGAATTGGTGACAAAGACTGTACAGCTCTGTAAAGGATTTCCACTTGCCATTACTGTGATCGGAAAAGATCTTTGCAACCAACCAGCAGAGATCTGGGAAAGAAGAGTGGCAGAATTGTCTAAAGGTTCTTCTATTCTTGATTGTGAGAAATTGTTGCTTGATCGACTACAGAGTAGCCTAGATGCTTTGGGTAAAGGAGCCACTTTAGTCAAGGAATGTTTCATAGACCTCGGTTCCTTTCCTGAAGACCGAAGAATCCCCGTTGCTGCCCTCATTGATATGTGGGCGGAGTTGCATGACCTACACTTGGATTCTTCATCCATAGCTTACCTGTATGATCTCAGCACCCGCAGTCTAGCGAACCTTATAGTCATCAGGAACGCAAAGGAGGATACAAATGGCTACTATAGCCAACATTTTGTTACCCAGCATGATATACTTAGGAGCCTGGCTATTCATCAGACTGGCCATGACACAATAGGACATAGCAAAAGGCTGATCATAGACATTTGTAAAGATGTTATTCCTAATTGGTGGACAGAACATAAAAGACCCACGAAGACTCGCTTATTATCTATCTCAACTGATGGGATGTTTTCATCAAAATGGCACAACATGCATCTGCCAAAAGCGAAAGTTCTGGTCTTGAATTTTCAGACAAAGAACTATGCATTTCCCAAGTTCAAGAAAACAATGTCAAAGTCGTTGAAGGTTCTAATAGTCACAAATTATGGTGTCACACCTGCCGAAGTGAGTAACTTTACATCATTGGGTTCTTTATCCGAGCTGAAGAGAATAAGACTAGAGCGCATTTTAGTCCCTCCTATTATCAAGAACTACATACAGTTGAATAGTCTACAAAAGTTATCTTTGTTCATGTGTAGCATCGGCCGAGCTTTTAGCAACGATTCCCTCAATTTTTTTGAAGCATTCCCAGGCCTTGAAGAAATCAACATTGACTATTGCAGTGATCTGATGAAATTACCTGCTGACCTTTGTCTTCTGAATCATCTAAGGAAGCTCAGTATCACCAACAGTCATAAATTATCTGACTTGCCTAAAGAAATTGGAAAACTGGAGAATTTAGAACTTTTGAGGTTAAGGTCATGCGCAGAATTGTCAGAGTTTCCGGGTTCGGTTAGGAAACTCAAAAAGTTGAAGGTTCTTGACATATCCAATTGCTACAGCATTAAGGAATTGCCTGAACATATTGGTGAACTGTGCAGCTTAGAAAAGCTCAACATGAGGCAGTGCTCAAGATTGCAAGAGTTGCCTGAATCAATTCTGGATCTTGAAAGTTTAACTGATGTGATATGCGATGAGGAGACAGAACGGCTATGGGAACCTTACTTGCTCAGTCTCAGAAACAACATAAAGATCAGGGTGGTCAAAGAGGAAATCAGCCTAGATTGGCTTCATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

824

Amino Acids

93.78

Weight (kDa)

8.33

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 11 - 121 5.2e-16 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 176 - 311 5.2e-15 NB-ARC domain
AAA_22 PF13401 192 - 283 6.9e-08 AAA domain
LRR_14 PF23598 659 - 742 2.7e-10 Leucine-rich repeat region
LRR_14 PF23598 744 - 793 6e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
AarI CACCTGC 1 cut(s) 1798
AasI GACNNNNNNGTC 1 cut(s) 145
AatII GACGTC 1 cut(s) 419
Acc16I TGCGCA 2 cut(s) 487, 2158
Acc36I ACCTGC 2 cut(s) 1798, 2041
AccI GTMKAC 1 cut(s) 1903
AciI CCGC 7 cut(s) 18, 280, 358, 500, 606, 1334, 1396
AclWI GGATC 3 cut(s) 738, 751, 2343
AcoI YGGCCR 2 cut(s) 1513, 1936
AcsI RAATTY 5 cut(s) 659, 758, 890, 1696, 2128
AcuI CTGAAG 3 cut(s) 846, 1311, 1856
AcyI GRCGYC 1 cut(s) 416
AfaI GTAC 2 cut(s) 492, 1068
AfiI CCNNNNNNNGG 2 cut(s) 203, 1354
AjnI CCWGG 3 cut(s) 595, 1494, 1981
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AloI GAACNNNNNNTCC 2 cut(s) 735, 767
Alw21I GWGCWC 5 cut(s) 132, 263, 588, 740, 2303
Alw26I GTCTC 3 cut(s) 407, 2366, 2411
AlwI GGATC 3 cut(s) 738, 751, 2343
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 5 cut(s) 205, 899, 1513, 1936, 1984
ApeKI GCWGC 6 cut(s) 41, 53, 64, 790, 1313, 2271
ApoI RAATTY 5 cut(s) 659, 758, 890, 1696, 2128
Asp700I GAANNNNTTC 1 cut(s) 2328
AspLEI GCGC 4 cut(s) 488, 781, 1857, 2159
AsuC2I CCSGG 1 cut(s) 2178
AsuHPI GGTGA 5 cut(s) 412, 931, 1066, 2068, 2273
AvaI CYCGRG 1 cut(s) 143
BalI TGGCCA 1 cut(s) 1515
BamHI GGATCC 1 cut(s) 743
BanII GRGCYC 1 cut(s) 132
BarI GAAGNNNNNNTAC 2 cut(s) 659, 691
BbsI GAAGAC 3 cut(s) 1299, 1613, 2039
Bbv12I GWGCWC 5 cut(s) 132, 263, 588, 740, 2303
BbvI GCAGC 6 cut(s) 28, 65, 76, 777, 1300, 2283
BccI CCATC 4 cut(s) 125, 223, 511, 1628
BceAI ACGGC 2 cut(s) 220, 2396
BciT130I CCWGG 3 cut(s) 597, 1496, 1983
BciVI GTATCC 1 cut(s) 1432
BclI TGATCA 2 cut(s) 565, 1545
BcnI CCSGG 1 cut(s) 2178
BcoDI GTCTC 3 cut(s) 407, 2366, 2411
BfaI CTAG 7 cut(s) 47, 209, 782, 1226, 1403, 1850, 2453
BfmI CTRYAG 4 cut(s) 1044, 1214, 1453, 2230
BfuAI ACCTGC 2 cut(s) 1798, 2041
BfuI GTATCC 1 cut(s) 1432
BglII AGATCT 2 cut(s) 1114, 1137
BisI GCNGC 6 cut(s) 42, 54, 65, 791, 1314, 2272
BlsI GCNGC 6 cut(s) 43, 55, 66, 792, 1315, 2273
BmcAI AGTACT 1 cut(s) 492
Bme1390I CCNGG 4 cut(s) 597, 1496, 1983, 2178
BmeT110I CYCGRG 1 cut(s) 143
BmiI GGNNCC 5 cut(s) 745, 1246, 1282, 1493, 2391
BmrFI CCNGG 4 cut(s) 597, 1496, 1983, 2178
BmsI GCATC 6 cut(s) 202, 593, 976, 1219, 1675, 1941
BoxI GACNNNNGTC 2 cut(s) 419, 2043
BpiI GAAGAC 3 cut(s) 1299, 1613, 2039
BpmI CTGGAG 2 cut(s) 618, 2144
BpuEI CTTGAG 1 cut(s) 2287
BpuMI CCSGG 1 cut(s) 2178
BsaHI GRCGYC 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 1276, 1981
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 1354
Bse1I ACTGG 4 cut(s) 12, 745, 1516, 2127
Bse3DI GCAATG 2 cut(s) 316, 996
BseBI CCWGG 3 cut(s) 597, 1496, 1983
BseDI CCNNGG 2 cut(s) 1276, 1981
BseGI GGATG 6 cut(s) 271, 529, 608, 847, 1364, 1644
BseLI CCNNNNNNNGG 2 cut(s) 203, 1354
BseMI GCAATG 2 cut(s) 316, 996
BseMII CTCAG 4 cut(s) 1402, 2083, 2416, 2422
BseNI ACTGG 4 cut(s) 12, 745, 1516, 2127
BseRI GAGGAG 2 cut(s) 248, 2384
BseX3I CGGCCG 1 cut(s) 1936
BseXI GCAGC 6 cut(s) 28, 65, 76, 777, 1300, 2283
BseYI CCCAGC 1 cut(s) 1473
BsgI GTGCAG 1 cut(s) 2290
Bsh1285I CGRYCG 1 cut(s) 1939
BshFI GGCC 5 cut(s) 207, 901, 1515, 1938, 1986
BsiEI CGRYCG 1 cut(s) 1939
BsiHKAI GWGCWC 5 cut(s) 132, 263, 588, 740, 2303
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 2177
BslFI GGGAC 2 cut(s) 979, 1850
BslI CCNNNNNNNGG 2 cut(s) 203, 1354
BsmAI GTCTC 3 cut(s) 407, 2366, 2411
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 979, 1850
BsmI GAATGC 1 cut(s) 1976
BsnI GGCC 5 cut(s) 207, 901, 1515, 1938, 1986
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 5 cut(s) 132, 263, 588, 740, 2303
Bsp1407I TGTACA 1 cut(s) 1066
BspACI CCGC 7 cut(s) 18, 280, 358, 500, 606, 1334, 1396
BspANI GGCC 5 cut(s) 207, 901, 1515, 1938, 1986
BspCNI CTCAG 4 cut(s) 1401, 2082, 2415, 2421
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 5 cut(s) 745, 1246, 1282, 1493, 2391
BspMI ACCTGC 2 cut(s) 1798, 2041
BspPI GGATC 3 cut(s) 738, 751, 2343
BspQI GCTCTTC 1 cut(s) 865
BsrDI GCAATG 2 cut(s) 316, 996
BsrGI TGTACA 1 cut(s) 1066
BsrI ACTGG 4 cut(s) 12, 745, 1516, 2127
BssECI CCNNGG 2 cut(s) 1276, 1981
BssNI GRCGYC 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 597, 1496, 1983
Bst4CI ACNGT 8 cut(s) 514, 573, 688, 1066, 1102, 1893, 2084, 2268
Bst6I CTCTTC 5 cut(s) 348, 359, 865, 1144, 1832
BstACI GRCGYC 1 cut(s) 416
BstAPI GCANNNNNTGC 2 cut(s) 1663, 2317
BstAUI TGTACA 1 cut(s) 1066
BstC8I GCNNGC 2 cut(s) 447, 788
BstDEI CTNAG 7 cut(s) 1388, 1487, 2060, 2069, 2275, 2402, 2408
BstF5I GGATG 6 cut(s) 271, 529, 608, 847, 1364, 1644
BstHHI GCGC 4 cut(s) 488, 781, 1857, 2159
BstMAI GTCTC 3 cut(s) 407, 2366, 2411
BstMCI CGRYCG 1 cut(s) 1939
BstMWI GCNNNNNNNGC 7 cut(s) 17, 50, 402, 787, 1455, 1663, 2317
BstNI CCWGG 3 cut(s) 597, 1496, 1983
BstNSI RCATGY 2 cut(s) 377, 1666
BstPAI GACNNNNGTC 2 cut(s) 419, 2043
BstSCI CCNGG 4 cut(s) 595, 1494, 1981, 2176
BstSFI CTRYAG 4 cut(s) 1044, 1214, 1453, 2230
BstV1I GCAGC 6 cut(s) 28, 65, 76, 777, 1300, 2283
BstV2I GAAGAC 3 cut(s) 1299, 1613, 2039
BstX2I RGATCY 4 cut(s) 743, 1114, 1137, 2335
BstXI CCANNNNNNTGG 1 cut(s) 740
BstYI RGATCY 4 cut(s) 743, 1114, 1137, 2335
BstZI CGGCCG 1 cut(s) 1936
BsuI GTATCC 1 cut(s) 1432
BsuRI GGCC 5 cut(s) 207, 901, 1515, 1938, 1986
BtgZI GCGATG 1 cut(s) 2379
BtsCI GGATG 6 cut(s) 271, 529, 608, 847, 1364, 1644
BtsI GCAGTG 3 cut(s) 588, 2020, 2303
BtsIMutI CAGTG 5 cut(s) 588, 684, 738, 2020, 2303
BveI ACCTGC 2 cut(s) 1798, 2041
Cac8I GCNNGC 2 cut(s) 447, 788
CciI TCATGA 1 cut(s) 886
CfoI GCGC 4 cut(s) 488, 781, 1857, 2159
Csp6I GTAC 2 cut(s) 491, 1067
CviAII CATG 9 cut(s) 374, 887, 1343, 1478, 1517, 1663, 1924, 2154, 2290
CviQI GTAC 2 cut(s) 491, 1067
DdeI CTNAG 7 cut(s) 1388, 1487, 2060, 2069, 2275, 2402, 2408
DraI TTTAAA 1 cut(s) 220
DrdI GACNNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 145
EaeI YGGCCR 2 cut(s) 1513, 1936
EagI CGGCCG 1 cut(s) 1936
Eam1104I CTCTTC 5 cut(s) 348, 359, 865, 1144, 1832
EarI CTCTTC 5 cut(s) 348, 359, 865, 1144, 1832
EciI GGCGGA 2 cut(s) 489, 1349
Ecl136II GAGCTC 1 cut(s) 130
EclXI CGGCCG 1 cut(s) 1936
Eco147I AGGCCT 1 cut(s) 1986
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 1039
Eco52I CGGCCG 1 cut(s) 1936
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 3 cut(s) 846, 1311, 1856
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 130
EcoRI GAATTC 2 cut(s) 758, 890
EcoRII CCWGG 3 cut(s) 595, 1494, 1981
EcoRV GATATC 1 cut(s) 1039
EcoT22I ATGCAT 3 cut(s) 375, 1668, 1720
EcoT38I GRGCYC 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 9 cut(s) 377, 890, 1346, 1481, 1520, 1666, 1927, 2157, 2293
FalI AAGNNNNNCTT 2 cut(s) 1598, 1630
FaqI GGGAC 2 cut(s) 979, 1850
FatI CATG 9 cut(s) 373, 886, 1342, 1477, 1516, 1662, 1923, 2153, 2289
FauI CCCGC 2 cut(s) 599, 1403
FauNDI CATATG 1 cut(s) 958
FbaI TGATCA 2 cut(s) 565, 1545
FblI GTMKAC 1 cut(s) 1903
Fnu4HI GCNGC 6 cut(s) 42, 54, 65, 791, 1314, 2272
FokI GGATG 6 cut(s) 258, 536, 615, 854, 1351, 1651
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 6 cut(s) 42, 54, 65, 791, 1314, 2272
FspBI CTAG 7 cut(s) 47, 209, 782, 1226, 1403, 1850, 2453
FspI TGCGCA 2 cut(s) 487, 2158
GlaI GCGC 4 cut(s) 487, 780, 1856, 2158
GluI GCNGC 6 cut(s) 42, 54, 65, 791, 1314, 2272
GsaI CCCAGC 1 cut(s) 1477
GsuI CTGGAG 2 cut(s) 618, 2144
HaeIII GGCC 5 cut(s) 207, 901, 1515, 1938, 1986
HapII CCGG 1 cut(s) 2177
HhaI GCGC 4 cut(s) 488, 781, 1857, 2159
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 9 cut(s) 377, 890, 1346, 1481, 1520, 1666, 1927, 2157, 2293
Hin6I GCGC 4 cut(s) 486, 779, 1855, 2157
HinP1I GCGC 4 cut(s) 486, 779, 1855, 2157
HinfI GANTC 9 cut(s) 146, 820, 866, 1302, 1358, 1609, 1955, 2053, 2324
HpaII CCGG 1 cut(s) 2177
HphI GGTGA 5 cut(s) 412, 931, 1066, 2068, 2273
Hpy166II GTNNAC 4 cut(s) 510, 1584, 1904, 2264
Hpy8I GTNNAC 4 cut(s) 510, 1584, 1904, 2264
Hpy99I CGWCG 1 cut(s) 421
HpyAV CCTTC 2 cut(s) 1750, 2200
HpyCH4III ACNGT 8 cut(s) 514, 573, 688, 1066, 1102, 1893, 2084, 2268
HpyCH4IV ACGT 3 cut(s) 416, 441, 705
HpyF10VI GCNNNNNNNGC 7 cut(s) 17, 50, 402, 787, 1455, 1663, 2317
HpyF3I CTNAG 7 cut(s) 1388, 1487, 2060, 2069, 2275, 2402, 2408
HpySE526I ACGT 3 cut(s) 416, 441, 705
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 9 cut(s) 377, 890, 1346, 1481, 1520, 1666, 1927, 2157, 2293
HspAI GCGC 4 cut(s) 486, 779, 1855, 2157
Ksp22I TGATCA 2 cut(s) 565, 1545
LguI GCTCTTC 1 cut(s) 865
LmnI GCTCC 6 cut(s) 50, 266, 598, 709, 1244, 1491
Lsp1109I GCAGC 6 cut(s) 28, 65, 76, 777, 1300, 2283
LweI GCATC 6 cut(s) 202, 593, 976, 1219, 1675, 1941
MaeI CTAG 7 cut(s) 47, 209, 782, 1226, 1403, 1850, 2453
MaeII ACGT 3 cut(s) 416, 441, 705
MaeIII GTNAC 9 cut(s) 22, 552, 682, 919, 1054, 1468, 1768, 1783, 1802
MfeI CAATTG 2 cut(s) 30, 2224
MflI RGATCY 4 cut(s) 743, 1114, 1137, 2335
MhlI GDGCHC 5 cut(s) 132, 263, 588, 740, 2303
MlsI TGGCCA 1 cut(s) 1515
MluNI TGGCCA 1 cut(s) 1515
MlyI GAGTC 2 cut(s) 155, 1603
MmeI TCCRAC 2 cut(s) 390, 496
Mox20I TGGCCA 1 cut(s) 1515
Mph1103I ATGCAT 3 cut(s) 375, 1668, 1720
MroXI GAANNNNTTC 1 cut(s) 2328
MscI TGGCCA 1 cut(s) 1515
MseI TTAA 7 cut(s) 165, 219, 495, 965, 2147, 2238, 2348
MslI CAYNNNNRTG 4 cut(s) 1100, 1325, 1651, 2259
Msp20I TGGCCA 1 cut(s) 1515
MspI CCGG 1 cut(s) 2177
MspR9I CCNGG 4 cut(s) 597, 1496, 1983, 2178
MunI CAATTG 2 cut(s) 30, 2224
Mva1269I GAATGC 1 cut(s) 1976
MvaI CCWGG 3 cut(s) 597, 1496, 1983
MwoI GCNNNNNNNGC 7 cut(s) 17, 50, 402, 787, 1455, 1663, 2317
NciI CCSGG 1 cut(s) 2178
NdeI CATATG 1 cut(s) 958
NlaIII CATG 9 cut(s) 377, 890, 1346, 1481, 1520, 1666, 1927, 2157, 2293
NlaIV GGNNCC 5 cut(s) 745, 1246, 1282, 1493, 2391
NmeAIII GCCGAG 1 cut(s) 1964
NmuCI GTSAC 5 cut(s) 682, 919, 1054, 1768, 1783
NsbI TGCGCA 2 cut(s) 487, 2158
NsiI ATGCAT 3 cut(s) 375, 1668, 1720
NspI RCATGY 2 cut(s) 377, 1666
PaeR7I CTCGAG 1 cut(s) 143
PagI TCATGA 1 cut(s) 886
PaqCI CACCTGC 1 cut(s) 1798
PceI AGGCCT 1 cut(s) 1986
PciSI GCTCTTC 1 cut(s) 865
PctI GAATGC 1 cut(s) 1976
PdmI GAANNNNTTC 1 cut(s) 2328
PfeI GAWTC 7 cut(s) 820, 866, 1302, 1358, 1955, 2053, 2324
PfoI TCCNGGA 1 cut(s) 595
PkrI GCNGC 6 cut(s) 43, 55, 66, 792, 1315, 2273
PleI GAGTC 2 cut(s) 154, 1603
PpsI GAGTC 2 cut(s) 154, 1603
PshAI GACNNNNGTC 2 cut(s) 419, 2043
PsiI TTATAA 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 3 cut(s) 595, 1494, 1981
PspFI CCCAGC 1 cut(s) 1473
PspGI CCWGG 3 cut(s) 595, 1494, 1981
PspN4I GGNNCC 5 cut(s) 745, 1246, 1282, 1493, 2391
PspXI VCTCGAGB 1 cut(s) 143
PsuI RGATCY 4 cut(s) 743, 1114, 1137, 2335
RsaI GTAC 2 cut(s) 492, 1068
RsaNI GTAC 2 cut(s) 491, 1067
RseI CAYNNNNRTG 4 cut(s) 1100, 1325, 1651, 2259
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 865
SaqAI TTAA 7 cut(s) 165, 219, 495, 965, 2147, 2238, 2348
SatI GCNGC 6 cut(s) 42, 54, 65, 791, 1314, 2272
ScaI AGTACT 1 cut(s) 492
SchI GAGTC 2 cut(s) 155, 1603
ScrFI CCNGG 4 cut(s) 597, 1496, 1983, 2178
SduI GDGCHC 5 cut(s) 132, 263, 588, 740, 2303
SfaNI GCATC 6 cut(s) 202, 593, 976, 1219, 1675, 1941
SfcI CTRYAG 4 cut(s) 1044, 1214, 1453, 2230
Sfr274I CTCGAG 1 cut(s) 143
SlaI CTCGAG 1 cut(s) 143
SmiMI CAYNNNNRTG 4 cut(s) 1100, 1325, 1651, 2259
SmlI CTYRAG 2 cut(s) 143, 2302
SmoI CTYRAG 2 cut(s) 143, 2302
SseBI AGGCCT 1 cut(s) 1986
SsiI CCGC 7 cut(s) 18, 280, 358, 500, 606, 1334, 1396
SspI AATATT 2 cut(s) 268, 673
SspMI CTAG 7 cut(s) 47, 209, 782, 1226, 1403, 1850, 2453
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 1 cut(s) 1986
StyD4I CCNGG 4 cut(s) 595, 1494, 1981, 2176
TaaI ACNGT 8 cut(s) 514, 573, 688, 1066, 1102, 1893, 2084, 2268
TaiI ACGT 3 cut(s) 419, 444, 708
TaqI TCGA 3 cut(s) 144, 427, 1210
TaqII GACCGA 2 cut(s) 409, 1311
TatI WGTACW 2 cut(s) 490, 1066
TfiI GAWTC 7 cut(s) 820, 866, 1302, 1358, 1955, 2053, 2324
Tru1I TTAA 7 cut(s) 165, 219, 495, 965, 2147, 2238, 2348
Tru9I TTAA 7 cut(s) 165, 219, 495, 965, 2147, 2238, 2348
TscAI CASTG 5 cut(s) 595, 691, 745, 2020, 2303
TseFI GTSAC 5 cut(s) 682, 919, 1054, 1768, 1783
TseI GCWGC 6 cut(s) 41, 53, 64, 790, 1313, 2271
Tsp45I GTSAC 5 cut(s) 682, 919, 1054, 1768, 1783
TspGWI ACGGA 1 cut(s) 992
TspRI CASTG 5 cut(s) 595, 691, 745, 2020, 2303
XapI RAATTY 5 cut(s) 659, 758, 890, 1696, 2128
XceI RCATGY 2 cut(s) 377, 1666
XcmI CCANNNNNNNNNTGG 1 cut(s) 1137
XhoI CTCGAG 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 1903
XmnI GAANNNNTTC 1 cut(s) 2328
XspI CTAG 7 cut(s) 47, 209, 782, 1226, 1403, 1850, 2453
ZraI GACGTC 1 cut(s) 417
ZrmI AGTACT 1 cut(s) 492
Zsp2I ATGCAT 3 cut(s) 375, 1668, 1720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.