RchiOBHm_Chr3g0485291

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000039
Physical Location & Seq
Forward (+)
31963632 .. 31971286
7655 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44991

Sequence Viewer

Length: 2283 bp
ATGGAAGCTGTTACGCTAATCGGAGGGGCTGCTCTAGGAACAGCATTTACGGCTCTGTATGAGGTCGTGAAGGAGAGTAAAGAGTATAAGCCAATGCTTAAACGCCTCAAGGGGAGGCTAACGTTTTTAGATCCAATGATTGCAGAGGTCAGAAGAACTAATACGATGCTGCAGGGAGTCGAAGATATTATGAAAGAAATGGATGCGGGCAGACAACTCATTCAAGAGTGTTCCAGGGCTCATTGGTTAACTGGCACGTACACGAGGTACGGGTACGCCTCAAAACTTCGCTCATTAAATAGAGCTCTTAAAGATCTGATGCAAATACTGATTGCATACGGTGTATTGCAATTGACTGAGAACAATAACGTTCTCACGAGAATTTGCCAAAGTCCACTACCTGAACCTCCACAGTTTACGGTTGGACTGGATAAGCCTTTAGGGGAATTGAAGAAGCTTATTGGGATGGATGGGGAGTCAATGGTTGTGATAACTGCTCCGGGAGGATGCGGGAAAACCACTTTGGCAACAAAGTTTTGCCATGATCAGGAAGTTAAAGACAAATACAAGAACATCTTCTTTGTCACTGTTTCAGAGAATCCCAACTGGGACCGTATTGTAGAACAACTTTTCCAACGAAAGGGTTGGGACTATAAATTGCTCAAAAACGAAAGAAATACTGATTCGTGGCTGGAATATTTTCTGCGAGCAGACCAACAACCTTCCCTGTTGGTCCTCGATGAAGTTTGGTTGGGATGGGAATCCCGTAAGCTTCTTGACAAGTTTGATCGATTCAAAAACTCGAATTGCAAAATTTTGGTGACATCAAGATTCCATTTTCCAAAGTTTCGTTCATATTATTTGAAGTCATTGAATGATGAAGATGCAAAGGAACTTTTTCATCATTCAGCCTTATTGGGAGAGAGGACCTGTGCTATTCCAGAAAGTCTTTCAAAAAAGGTACTCGAGTTTTGTAAGGGATTACCGTTTGCCATTACAGTGGTTGGAGAATCACTTGCTCGGCAGGAGAGGGAGATCTGGGAAAGGGTTACGAAAGGTTCTATTCTTAAATTGGAGATGGTTGTACATAATCGACTCGAAAGCAGCTTAAAGGCCTTGGAATCAGAAAATCCTCTCTTAAAAGAATGTTTCCTAGACCTTGCTTTATTTTTTGAAGATCGATTAATCTCTGCTGCTGCACTCATTGATTTGTGGTCAGAGTTATACGACGATCTAAATGAGGACAGTTCGTGCATTGCAAACCTCACCGACCTCAAAAATAGGACTCTTGCTAATCTTGTATTCCCAAGAGATGAGTACACCTATGGCTACTACAGTGAACACTTCGTTACTCAACATGAAATGCTTCGTGAGCTGGCTCTTTATCACACAAGTCTGGTCCGGATAAGAGAGAGAAAAAGGCTAATTATGAACATCTGTGGCGACAATTTCCCCAGATGGAAGGAAGAACTGATGTGTCAACCTCAACCTCTCGAGGCTCACCTATTATCTATCACAACTGATCAAGCATTCTCAACCGAATGGTGCAATATTCAACCACCAAGAGCTGAGGTTCTAGTTTTGAATTGTCAAACAAAATGCTGTGCCTTGCCAAGACCTGTGCAGACAGAAGATTATACTTTACCTAAATTTGGGGGTAAAATAGATAACTTGAAGGTTTTAATAGTCACATATAATGGTTACTTACCTGCTACATTAAGTAATCCTCTGCTATTGGGTTCGATGTCAAATTTGAAGAGAATGAGATTAGAGCGCATTTCAATCATAATCCAGAACGGCTTAGCGCTGAAGAGTCTTTGTAAGATGTCGTTCTTCATGTGCAACATCAGTCAAACTTTCCGTGGAAGTTCCATCCAATTTTCAGATGCTTTTCCGAATCTAGTGGAACTTAACATTGACTATTGCAATGATTTGGTGGAATTGCCTGCCAGCCTCTGTGATCATATTGTGCTGAAGAAACTCGGTATCACGAACTGTCATATGCTATCTACCTTGCCTGAGGAGATTGGAAACTCGATCAACTTAGAGGAGTTGAGGCTATTGGCTTGTACAAAGTTGTCAGAGTTGCCCAACTCAATTACGATGCTCACGAAGTTGAAACTGCTTGACATATCTTGTTGCCGGAGTATTACAAAATTGCCTGATTGCATTGGTAAATTGGGCAGTTTAAGAAACCTCATCATGAGACATTGCTCAAATTTGCTAACGCTGCCTACAACAATTCTCGATCTTAATCACTTAGAGGAACTCCAATGGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

760

Amino Acids

87.0

Weight (kDa)

6.91

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 111 2.9e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 151 - 278 5.7e-14 NB-ARC domain
AAA_22 PF13401 158 - 249 5.6e-06 AAA domain
LRR_14 PF23598 629 - 758 9.2e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1717
AccIII TCCGGA 1 cut(s) 1401
AciI CCGC 2 cut(s) 206, 510
AclI AACGTT 2 cut(s) 122, 369
AclWI GGATC 1 cut(s) 125
AcsI RAATTY 5 cut(s) 381, 813, 1649, 1750, 2218
AcuI CTGAAG 2 cut(s) 1829, 1994
AfaI GTAC 7 cut(s) 260, 269, 275, 963, 1086, 1319, 2071
AfeI AGCGCT 1 cut(s) 1806
AfiI CCNNNNNNNGG 3 cut(s) 547, 640, 1652
AjnI CCWGG 1 cut(s) 233
AjuI GAANNNNNNNTTGG 4 cut(s) 506, 538, 835, 867
AloI GAACNNNNNNTCC 2 cut(s) 615, 647
AluBI AGCT 7 cut(s) 8, 305, 457, 772, 1107, 1375, 1568
AluI AGCT 7 cut(s) 8, 305, 457, 772, 1107, 1375, 1568
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 1 cut(s) 2200
AlwI GGATC 1 cut(s) 125
AlwNI CAGNNNCTG 1 cut(s) 1956
Ama87I CYCGRG 2 cut(s) 965, 1493
Aor13HI TCCGGA 1 cut(s) 1401
Aor51HI AGCGCT 1 cut(s) 1806
AoxI GGCC 2 cut(s) 1113, 2276
ApeKI GCWGC 6 cut(s) 29, 169, 1104, 1193, 1196, 2230
ApoI RAATTY 5 cut(s) 381, 813, 1649, 1750, 2218
AseI ATTAAT 1 cut(s) 1184
Asp700I GAANNNNTTC 4 cut(s) 575, 699, 897, 1365
AspLEI GCGC 2 cut(s) 1776, 1807
AspS9I GGNCC 5 cut(s) 610, 733, 927, 1399, 2277
AsuC2I CCSGG 1 cut(s) 501
AsuHPI GGTGA 3 cut(s) 832, 1258, 1493
AvaI CYCGRG 2 cut(s) 965, 1493
AvaII GGWCC 4 cut(s) 610, 733, 927, 1399
AxyI CCTNAGG 1 cut(s) 2019
BaeI ACNNNNGTAYC 2 cut(s) 953, 986
BanII GRGCYC 2 cut(s) 241, 307
BarI GAAGNNNNNNTAC 2 cut(s) 145, 177
BauI CACGAG 2 cut(s) 262, 376
Bbv12I GWGCWC 1 cut(s) 307
BbvCI CCTCAGC 1 cut(s) 1569
BbvI GCAGC 6 cut(s) 16, 156, 1116, 1180, 1183, 2217
BccI CCATC 6 cut(s) 460, 464, 750, 1072, 1452, 1880
BceAI ACGGC 2 cut(s) 66, 1813
BciT130I CCWGG 1 cut(s) 235
BclI TGATCA 3 cut(s) 544, 1522, 1960
BcnI CCSGG 1 cut(s) 501
BcoDI GTCTC 1 cut(s) 2200
BfaI CTAG 4 cut(s) 35, 1154, 1577, 1901
BfmI CTRYAG 2 cut(s) 170, 1333
BfoI RGCGCY 1 cut(s) 1808
BfuAI ACCTGC 1 cut(s) 1717
BglII AGATCT 2 cut(s) 313, 1035
BisI GCNGC 6 cut(s) 30, 170, 1105, 1194, 1197, 2231
BlpI GCTNAGC 1 cut(s) 1801
BlsI GCNGC 6 cut(s) 31, 171, 1106, 1195, 1198, 2232
Bme1390I CCNGG 2 cut(s) 235, 501
Bme18I GGWCC 4 cut(s) 610, 733, 927, 1399
BmeT110I CYCGRG 2 cut(s) 965, 1493
BmgT120I GGNCC 5 cut(s) 610, 733, 927, 1399, 2277
BmiI GGNNCC 1 cut(s) 611
BmrFI CCNGG 2 cut(s) 235, 501
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 7 cut(s) 156, 193, 309, 497, 874, 1876, 2094
BmuI ACTGGG 1 cut(s) 616
Bpu10I CCTNAGC 1 cut(s) 1569
Bpu1102I GCTNAGC 1 cut(s) 1801
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 501
Bsa29I ATCGAT 2 cut(s) 790, 1180
BsaAI YACGTR 1 cut(s) 258
BsaBI GATNNNNATC 2 cut(s) 760, 2253
BsaJI CCNNGG 3 cut(s) 234, 1116, 1861
BsaWI WCCGGW 1 cut(s) 1401
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 640, 1652
Bse1I ACTGG 3 cut(s) 256, 432, 611
Bse21I CCTNAGG 1 cut(s) 2019
Bse3DI GCAATG 3 cut(s) 1254, 1933, 2209
Bse8I GATNNNNATC 2 cut(s) 760, 2253
BseAI TCCGGA 1 cut(s) 1401
BseBI CCWGG 1 cut(s) 235
BseCI ATCGAT 2 cut(s) 790, 1180
BseDI CCNNGG 3 cut(s) 234, 1116, 1861
BseGI GGATG 6 cut(s) 208, 471, 475, 512, 761, 1872
BseJI GATNNNNATC 2 cut(s) 760, 2253
BseLI CCNNNNNNNGG 3 cut(s) 547, 640, 1652
BseMI GCAATG 3 cut(s) 1254, 1933, 2209
BseMII CTCAG 3 cut(s) 348, 1560, 2010
BseNI ACTGG 3 cut(s) 256, 432, 611
BseRI GAGGAG 2 cut(s) 2036, 2063
BseXI GCAGC 6 cut(s) 16, 156, 1116, 1180, 1183, 2217
BsgI GTGCAG 2 cut(s) 1182, 1643
BshFI GGCC 2 cut(s) 1115, 2278
BshVI ATCGAT 2 cut(s) 790, 1180
BsiHKAI GWGCWC 1 cut(s) 307
BsiHKCI CYCGRG 2 cut(s) 965, 1493
BsiSI CCGG 3 cut(s) 500, 1402, 2143
BslFI GGGAC 2 cut(s) 623, 662
BslI CCNNNNNNNGG 3 cut(s) 547, 640, 1652
BsmAI GTCTC 1 cut(s) 2200
BsmFI GGGAC 2 cut(s) 623, 662
BsmI GAATGC 1 cut(s) 1529
BsnI GGCC 2 cut(s) 1115, 2278
BsoBI CYCGRG 2 cut(s) 965, 1493
Bsp1286I GDGCHC 2 cut(s) 241, 307
Bsp13I TCCGGA 1 cut(s) 1401
Bsp1407I TGTACA 2 cut(s) 1084, 2069
Bsp1720I GCTNAGC 1 cut(s) 1801
BspACI CCGC 2 cut(s) 206, 510
BspANI GGCC 2 cut(s) 1115, 2278
BspCNI CTCAG 3 cut(s) 349, 1561, 2011
BspDI ATCGAT 2 cut(s) 790, 1180
BspEI TCCGGA 1 cut(s) 1401
BspHI TCATGA 1 cut(s) 2202
BspLI GGNNCC 1 cut(s) 611
BspMAI CTGCAG 1 cut(s) 174
BspMI ACCTGC 1 cut(s) 1717
BspPI GGATC 1 cut(s) 125
BsrDI GCAATG 3 cut(s) 1254, 1933, 2209
BsrGI TGTACA 2 cut(s) 1084, 2069
BsrI ACTGG 3 cut(s) 256, 432, 611
BssECI CCNNGG 3 cut(s) 234, 1116, 1861
BssSI CACGAG 2 cut(s) 262, 376
BssT1I CCWWGG 1 cut(s) 1116
Bst2BI CACGAG 2 cut(s) 262, 376
Bst2UI CCWGG 1 cut(s) 235
Bst6I CTCTTC 2 cut(s) 1751, 1805
BstAUI TGTACA 2 cut(s) 1084, 2069
BstBAI YACGTR 1 cut(s) 258
BstC8I GCNNGC 5 cut(s) 208, 708, 1377, 1947, 1951
BstDEI CTNAG 6 cut(s) 357, 1569, 1801, 2019, 2044, 2260
BstDSI CCRYGG 1 cut(s) 1861
BstF5I GGATG 6 cut(s) 208, 471, 475, 512, 761, 1872
BstH2I RGCGCY 1 cut(s) 1808
BstHHI GCGC 2 cut(s) 1776, 1807
BstMAI GTCTC 1 cut(s) 2200
BstMWI GCNNNNNNNGC 3 cut(s) 50, 1372, 2230
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 233, 499
BstSFI CTRYAG 2 cut(s) 170, 1333
BstV1I GCAGC 6 cut(s) 16, 156, 1116, 1180, 1183, 2217
BstX2I RGATCY 3 cut(s) 130, 313, 1035
BstXI CCANNNNNNTGG 1 cut(s) 1000
BstYI RGATCY 3 cut(s) 130, 313, 1035
Bsu15I ATCGAT 2 cut(s) 790, 1180
Bsu36I CCTNAGG 1 cut(s) 2019
BsuRI GGCC 2 cut(s) 1115, 2278
BsuTUI ATCGAT 2 cut(s) 790, 1180
BtgI CCRYGG 1 cut(s) 1861
BtsCI GGATG 6 cut(s) 208, 471, 475, 512, 761, 1872
BtsIMutI CAGTG 3 cut(s) 585, 1005, 1342
BveI ACCTGC 1 cut(s) 1717
Cac8I GCNNGC 5 cut(s) 208, 708, 1377, 1947, 1951
CaiI CAGNNNCTG 1 cut(s) 1956
CciI TCATGA 1 cut(s) 2202
CfoI GCGC 2 cut(s) 1776, 1807
Cfr13I GGNCC 5 cut(s) 610, 733, 927, 1399, 2277
ClaI ATCGAT 2 cut(s) 790, 1180
Csp6I GTAC 7 cut(s) 259, 268, 274, 962, 1085, 1318, 2070
CviAII CATG 4 cut(s) 542, 1358, 1837, 2203
CviQI GTAC 7 cut(s) 259, 268, 274, 962, 1085, 1318, 2070
DdeI CTNAG 6 cut(s) 357, 1569, 1801, 2019, 2044, 2260
Eam1104I CTCTTC 2 cut(s) 1751, 1805
EarI CTCTTC 2 cut(s) 1751, 1805
Ecl136II GAGCTC 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 1116
Eco147I AGGCCT 1 cut(s) 1115
Eco24I GRGCYC 2 cut(s) 241, 307
Eco47I GGWCC 4 cut(s) 610, 733, 927, 1399
Eco47III AGCGCT 1 cut(s) 1806
Eco53kI GAGCTC 1 cut(s) 305
Eco57I CTGAAG 2 cut(s) 1829, 1994
Eco81I CCTNAGG 1 cut(s) 2019
Eco88I CYCGRG 2 cut(s) 965, 1493
EcoICRI GAGCTC 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 927
EcoRII CCWGG 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 1116
EcoT38I GRGCYC 2 cut(s) 241, 307
ErhI CCWWGG 1 cut(s) 1116
FaeI CATG 4 cut(s) 545, 1361, 1840, 2206
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 2 cut(s) 623, 662
FatI CATG 4 cut(s) 541, 1357, 1836, 2202
FauI CCCGC 2 cut(s) 199, 503
FauNDI CATATG 1 cut(s) 2001
FbaI TGATCA 3 cut(s) 544, 1522, 1960
Fnu4HI GCNGC 6 cut(s) 30, 170, 1105, 1194, 1197, 2231
FokI GGATG 6 cut(s) 215, 478, 482, 519, 768, 1859
FriOI GRGCYC 2 cut(s) 241, 307
Fsp4HI GCNGC 6 cut(s) 30, 170, 1105, 1194, 1197, 2231
FspBI CTAG 4 cut(s) 35, 1154, 1577, 1901
GlaI GCGC 2 cut(s) 1775, 1806
GluI GCNGC 6 cut(s) 30, 170, 1105, 1194, 1197, 2231
HaeII RGCGCY 1 cut(s) 1808
HaeIII GGCC 2 cut(s) 1115, 2278
HapII CCGG 3 cut(s) 500, 1402, 2143
HhaI GCGC 2 cut(s) 1776, 1807
Hin1II CATG 4 cut(s) 545, 1361, 1840, 2206
Hin6I GCGC 2 cut(s) 1774, 1805
HinP1I GCGC 2 cut(s) 1774, 1805
HincII GTYRAC 2 cut(s) 249, 1481
HindII GTYRAC 2 cut(s) 249, 1481
HindIII AAGCTT 2 cut(s) 455, 770
HpaI GTTAAC 1 cut(s) 249
HpaII CCGG 3 cut(s) 500, 1402, 2143
HphI GGTGA 3 cut(s) 832, 1258, 1493
Hpy166II GTNNAC 7 cut(s) 249, 261, 395, 417, 1320, 1340, 1481
Hpy188I TCNGA 9 cut(s) 23, 152, 318, 595, 1126, 1219, 1885, 1896, 2083
Hpy8I GTNNAC 7 cut(s) 249, 261, 395, 417, 1320, 1340, 1481
Hpy99I CGWCG 1 cut(s) 1232
HpyAV CCTTC 4 cut(s) 64, 732, 1456, 1669
HpyCH4IV ACGT 3 cut(s) 122, 257, 369
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 1372, 2230
HpyF3I CTNAG 6 cut(s) 357, 1569, 1801, 2019, 2044, 2260
HpySE526I ACGT 3 cut(s) 122, 257, 369
Hsp92II CATG 4 cut(s) 545, 1361, 1840, 2206
HspAI GCGC 2 cut(s) 1774, 1805
Kpn2I TCCGGA 1 cut(s) 1401
Ksp22I TGATCA 3 cut(s) 544, 1522, 1960
KspAI GTTAAC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 502
Lsp1109I GCAGC 6 cut(s) 16, 156, 1116, 1180, 1183, 2217
LweI GCATC 7 cut(s) 156, 193, 309, 497, 874, 1876, 2094
MaeI CTAG 4 cut(s) 35, 1154, 1577, 1901
MaeII ACGT 3 cut(s) 122, 257, 369
MaeIII GTNAC 7 cut(s) 10, 583, 820, 1048, 1348, 1687, 1700
MfeI CAATTG 1 cut(s) 350
MflI RGATCY 3 cut(s) 130, 313, 1035
MhlI GDGCHC 2 cut(s) 241, 307
MlyI GAGTC 5 cut(s) 186, 485, 1089, 1279, 1822
MmeI TCCRAC 3 cut(s) 403, 658, 985
MroI TCCGGA 1 cut(s) 1401
MroXI GAANNNNTTC 4 cut(s) 575, 699, 897, 1365
MslI CAYNNNNRTG 1 cut(s) 998
MspI CCGG 3 cut(s) 500, 1402, 2143
MspR9I CCNGG 2 cut(s) 235, 501
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 1529
MvaI CCWGG 1 cut(s) 235
MwoI GCNNNNNNNGC 3 cut(s) 50, 1372, 2230
NciI CCSGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 2001
NlaIII CATG 4 cut(s) 545, 1361, 1840, 2206
NlaIV GGNNCC 1 cut(s) 611
NmeAIII GCCGAG 1 cut(s) 1000
NmuCI GTSAC 3 cut(s) 583, 820, 1687
PaeR7I CTCGAG 2 cut(s) 965, 1493
PagI TCATGA 1 cut(s) 2202
PceI AGGCCT 1 cut(s) 1115
PcsI WCGNNNNNNNCGW 1 cut(s) 2108
PctI GAATGC 1 cut(s) 1529
PdmI GAANNNNTTC 4 cut(s) 575, 699, 897, 1365
PfeI GAWTC 8 cut(s) 598, 683, 761, 792, 831, 1010, 1121, 1897
PfoI TCCNGGA 1 cut(s) 499
PkrI GCNGC 6 cut(s) 31, 171, 1106, 1195, 1198, 2232
PleI GAGTC 5 cut(s) 185, 484, 1089, 1279, 1821
PpsI GAGTC 5 cut(s) 185, 484, 1089, 1279, 1821
Ppu21I YACGTR 1 cut(s) 258
PpuMI RGGWCCY 1 cut(s) 927
PshBI ATTAAT 1 cut(s) 1184
Psp124BI GAGCTC 1 cut(s) 307
Psp1406I AACGTT 2 cut(s) 122, 369
Psp5II RGGWCCY 1 cut(s) 927
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 611
PspPI GGNCC 5 cut(s) 610, 733, 927, 1399, 2277
PspPPI RGGWCCY 1 cut(s) 927
PspXI VCTCGAGB 1 cut(s) 965
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 1956
PsuI RGATCY 3 cut(s) 130, 313, 1035
RsaI GTAC 7 cut(s) 260, 269, 275, 963, 1086, 1319, 2071
RsaNI GTAC 7 cut(s) 259, 268, 274, 962, 1085, 1318, 2070
RseI CAYNNNNRTG 1 cut(s) 998
SacI GAGCTC 1 cut(s) 307
SatI GCNGC 6 cut(s) 30, 170, 1105, 1194, 1197, 2231
Sau96I GGNCC 5 cut(s) 610, 733, 927, 1399, 2277
SchI GAGTC 5 cut(s) 186, 485, 1089, 1279, 1822
ScrFI CCNGG 2 cut(s) 235, 501
SduI GDGCHC 2 cut(s) 241, 307
SfaNI GCATC 7 cut(s) 156, 193, 309, 497, 874, 1876, 2094
SfcI CTRYAG 2 cut(s) 170, 1333
Sfr274I CTCGAG 2 cut(s) 965, 1493
SinI GGWCC 4 cut(s) 610, 733, 927, 1399
SlaI CTCGAG 2 cut(s) 965, 1493
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 3 cut(s) 107, 965, 1493
SmoI CTYRAG 3 cut(s) 107, 965, 1493
SseBI AGGCCT 1 cut(s) 1115
SsiI CCGC 2 cut(s) 206, 510
SspI AATATT 2 cut(s) 698, 1552
SspMI CTAG 4 cut(s) 35, 1154, 1577, 1901
SstI GAGCTC 1 cut(s) 307
StuI AGGCCT 1 cut(s) 1115
StyD4I CCNGG 2 cut(s) 233, 499
StyI CCWWGG 1 cut(s) 1116
TaiI ACGT 3 cut(s) 125, 260, 372
TatI WGTACW 3 cut(s) 1084, 1317, 2069
TfiI GAWTC 8 cut(s) 598, 683, 761, 792, 831, 1010, 1121, 1897
TscAI CASTG 3 cut(s) 592, 1005, 1342
TseFI GTSAC 3 cut(s) 583, 820, 1687
TseI GCWGC 6 cut(s) 29, 169, 1104, 1193, 1196, 2230
Tsp45I GTSAC 3 cut(s) 583, 820, 1687
TspDTI ATGAA 8 cut(s) 206, 756, 843, 890, 894, 1374, 1445, 1825
TspGWI ACGGA 1 cut(s) 1850
TspRI CASTG 3 cut(s) 592, 1005, 1342
VpaK11BI GGWCC 4 cut(s) 610, 733, 927, 1399
VspI ATTAAT 1 cut(s) 1184
XapI RAATTY 5 cut(s) 381, 813, 1649, 1750, 2218
XhoI CTCGAG 2 cut(s) 965, 1493
XmnI GAANNNNTTC 4 cut(s) 575, 699, 897, 1365
XspI CTAG 4 cut(s) 35, 1154, 1577, 1901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.