Rw4G014030

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000039
Physical Location & Seq
Reverse (-)
32746260 .. 32749569
3310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014030.1

Sequence Viewer

Length: 2547 bp
ATGGTTGTAGATGTTGCCGCATCTGCTGTAGGAGTAGCATTATCGCTGCTGTGTAAGGCCATTGAAGAAGTTATCAGAAAGTGCGTGATCGACAAGATTGTGCAGTTCAATCCCCTGCTCGAGAAGATCAATACCAAGCTGAAGACAGTGAAGCCAGGGATAGAAGAGATCGAGAAAAGCAATCTAGAATTGGATCGCACAGGTGAATTGACAGATTTCAAACAAGTAATCGAGGATATCACAGCGCTTGTTCTTAAGTTACAAGAGGTTCCCCATTGGTACAAGAAACCTAAATACACCGACAAACTCAAAGAACTCAATGAATCTCTGGATACACAGATAATGTTACTGCAGGTGCCGACAGCAAGTAAGGTCTCTAACCTTTCGGTTATAACAAGTATTATCGAGGGGGACACCAGAAATATCCGTATGCAAATGGGGAATATGAGTGTGCAAGTGGGGAGTATGAGTATGCAAATAGAGGATTTGAATACGAACCTTGAGATGATCAGGGATATGCGACAGAATGGCCTAGTGCAAAATCAAGTTAAAGGTTGTTGGTCTGGAGTACCTGAACCGCCATCATTTACAGTTGGATTGGATTTACGTTTGGATGAATTGAAGAAGAAACTTCTTGAGGATGGGGTGTCAATGGGTCTGGTCACTGGTCATCCAGGCTCTGGGAAAACCACTTTGGCAAAAGCTCTTTGTCAAGATAAGAAAGTGAAAGACAAATTCAAGGGCATCATCTTTGTCGACGTTTCAAAAAGACGCAACACTGAGGTTGTGCAGGAAATATGTGAAAAAAAGGGTACCCAAATACTTTCTTTCCAATCCGAAGAAATTGCACTTGACAGGCTGCAACAACTTCTGAATAACACAGAAGAAGATCCTGTGCTTTTGGTCCTGGATAACGTTTGGCCCGGAAACAGGACAGAATCCCTCCTGGCGAGCTTTAAGTTCAAAGAATCGGAAATGTCAAATTACAAGATTTTGGTGACATCAAGATATATGTTGCCAGGATTTGATGTTGAATACCACGTAGAAGAATTGAATGACAATGATGCAATGAGTCTTCTCCGTAATTCAGCACGCCTCACAAGTTCTTCTTCTATTCCAGAAGATCTTCAGAGAAGACTTCCACTGGCTATTGAAGTGGTCGGAAGATCACTTTTCGGCAAGAATATTGTGATCTGGAAAGATAGAGTAGAAGCATGGTCTAGCGGTTCTTCTGTCATTAAGTCTGAAAAACATTTGCTTGTTTGCCTCCAAAGTAGTTTAGATGCCCTGGGTGAAGAAAATGCCCTCATCAAGGAATGTTTCATGGACCTTGCTTCATTTCCTCAAGGCCGAAGGATTCCTGCTTCTGTCCTGATTGATATCTGGAGTGTCCTCATTGATATGTGGGCTGCGGATAAAGATAGTTCATGTGTTGCAAAACTCTACGAGCTCACCTACTGGAGGCTGGCTAATCTTGTAATCACGAGGAATGAGAAGGGGGAGGTGGATGACTACTATGGTGACCACTTCGTTACCCAACATGATCTCCTTAGAGAGCTGGCTGTCCACCAGACAGATGAGGGCCCTGTAAAACTGAGGAGAAGACTGATTATAAACATACGTGGAAACAAACTTCCTAAATGGTGGAAAAAACAGATGGATCAGCCCTTCGAAGAAGTTCTCCTAGTATCTATTTCAACTGATAGTCTCTTCTCATCAAAATGGCGCGACATACAGCTACCGAAAGCTAAAGTTCTAGTTCTGAATTTCCACACAAAGAACTATGCATTGCCCGAATTTGTGAAGAAAATGGAGAAATTGAAGGTTCTAATAGTCACAAATTATGGGTTCTTACCGGCTGAAGTGAGTAATTTTACATTGCTGGGTTCTTCATCTAAACTAAAGAGGATAAGATTAGAGCGGATTTCTCTTTCTTCCATTACCAAGAACCCCATAGTATTGAAGAGACTACAGAAGATATCTTTCTTCATGTGTAACATGGGGCAAGCTTTTAGCAAAGGTTCCATCAAATTTTTTGAGGCATTCCCAGACCTTCAAGAAATCAACATTGACTATTGCAGTGATTTGGTGGAATTACCTGCTGACATCTGTTTTCTAATCCATCTGAAAAAGCTCAGCATCACCAACTGTCATAAGCTATCTGCCTTGCCTGAAATGATTGGAGAGCTAGTCAATTTAGAAGTTTTGAGGTTAAGGTCCTGCACAGACTTGTCCGAGCTGCCAAGTTCAGTTAGGAAACTCAAAAAACTAAAGGTTCTTGACATATCCAATTGCTACAGCATTAAGGAGTTGCCTGAACACATTGGTGAAATGTGCTGCTTACAAAAGCTCAACATGAGGCAGTGCTCGAGAATCCAAGAGCTGCCTGAATCAATTTTGGATCTTGAAACGTTGACTGATGTGATATGTGATGAAGAGACAAAATTGTTTTGGGAAACTTACTCGCCCTGTCTCAGCAACCACATACAGATAACGGCGGTCAAAGAGGAAATCAACCTAGATTGGCTTTATATGCGTCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

848

Amino Acids

96.59

Weight (kDa)

6.32

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 112 8.9e-13 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 204 - 363 7.5e-17 NB-ARC domain
AAA_22 PF13401 217 - 306 3.3e-08 AAA domain
LRR_14 PF23598 702 - 765 2e-10 Leucine-rich repeat region
LRR_14 PF23598 757 - 816 4.9e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 392, 1613
AarI CACCTGC 1 cut(s) 343
Acc36I ACCTGC 2 cut(s) 343, 2107
Acc65I GGTACC 1 cut(s) 812
AccB1I GGYRCC 2 cut(s) 355, 812
AccB7I CCANNNNNTGG 1 cut(s) 680
AccBSI CCGCTC 1 cut(s) 1921
AccI GTMKAC 1 cut(s) 756
AccII CGCG 1 cut(s) 1728
AciI CCGC 6 cut(s) 18, 578, 1224, 1412, 1921, 2498
AclI AACGTT 2 cut(s) 915, 2411
AclWI GGATC 4 cut(s) 201, 884, 1668, 2409
AcsI RAATTY 4 cut(s) 734, 1765, 1796, 2030
AcuI CTGAAG 3 cut(s) 161, 1112, 1881
AfaI GTAC 3 cut(s) 281, 570, 814
AfeI AGCGCT 1 cut(s) 246
AfiI CCNNNNNNNGG 4 cut(s) 680, 930, 1312, 1461
AflII CTTAAG 1 cut(s) 254
AjnI CCWGG 6 cut(s) 154, 673, 906, 945, 1018, 1287
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
AleI CACNNNNGTG 1 cut(s) 2325
Alw21I GWGCWC 2 cut(s) 1452, 2369
Alw26I GTCTC 5 cut(s) 379, 1712, 1960, 2432, 2477
AlwI GGATC 4 cut(s) 201, 884, 1668, 2409
AlwNI CAGNNNCTG 1 cut(s) 680
Ama87I CYCGRG 2 cut(s) 119, 2368
Aor51HI AGCGCT 1 cut(s) 246
AoxI GGCC 5 cut(s) 57, 529, 920, 1348, 1582
ApaI GGGCCC 1 cut(s) 1586
ApeKI GCWGC 6 cut(s) 46, 859, 1409, 2239, 2337, 2383
ApoI RAATTY 4 cut(s) 734, 1765, 1796, 2030
Asp700I GAANNNNTTC 2 cut(s) 1125, 1677
Asp718I GGTACC 1 cut(s) 812
AspLEI GCGC 2 cut(s) 247, 1728
AspS9I GGNCC 6 cut(s) 904, 921, 1327, 1582, 1583, 2217
AsuC2I CCSGG 1 cut(s) 924
AsuHPI GGTGA 7 cut(s) 215, 1009, 1304, 1444, 1532, 2134, 2339
AsuII TTCGAA 1 cut(s) 1671
AvaI CYCGRG 2 cut(s) 119, 2368
AvaII GGWCC 3 cut(s) 904, 1327, 2217
BaeGI GKGCMC 1 cut(s) 1586
BanI GGYRCC 2 cut(s) 355, 812
BanII GRGCYC 2 cut(s) 1452, 1586
BauI CACGAG 1 cut(s) 1483
BbsI GAAGAC 4 cut(s) 149, 1067, 1141, 1609
Bbv12I GWGCWC 2 cut(s) 1452, 2369
BbvI GCAGC 6 cut(s) 33, 846, 1396, 2226, 2324, 2370
BccI CCATC 5 cut(s) 589, 635, 1651, 2033, 2130
BceAI ACGGC 1 cut(s) 2511
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BciT130I CCWGG 6 cut(s) 156, 675, 908, 947, 1020, 1289
BciVI GTATCC 1 cut(s) 325
BclI TGATCA 1 cut(s) 507
BcnI CCSGG 1 cut(s) 924
BcoDI GTCTC 5 cut(s) 379, 1712, 1960, 2432, 2477
BfaI CTAG 7 cut(s) 185, 533, 1221, 1685, 1757, 2189, 2519
BfmI CTRYAG 4 cut(s) 27, 350, 1970, 2296
BfoI RGCGCY 1 cut(s) 248
BfrI CTTAAG 1 cut(s) 254
BfuAI ACCTGC 2 cut(s) 343, 2107
BfuI GTATCC 1 cut(s) 325
BglII AGATCT 1 cut(s) 1123
BisI GCNGC 7 cut(s) 18, 47, 860, 1410, 2240, 2338, 2384
BlpI GCTNAGC 1 cut(s) 2135
BlsI GCNGC 7 cut(s) 19, 48, 861, 1411, 2241, 2339, 2385
Bme1390I CCNGG 7 cut(s) 156, 675, 908, 924, 947, 1020, 1289
Bme18I GGWCC 3 cut(s) 904, 1327, 2217
BmeT110I CYCGRG 2 cut(s) 119, 2368
BmgT120I GGNCC 6 cut(s) 904, 921, 1327, 1582, 1583, 2217
BmiI GGNNCC 5 cut(s) 270, 357, 814, 1584, 2023
BmrFI CCNGG 7 cut(s) 156, 675, 908, 924, 947, 1020, 1289
BmsI GCATC 5 cut(s) 29, 753, 1054, 1273, 2148
BpiI GAAGAC 4 cut(s) 149, 1067, 1141, 1609
BpmI CTGGAG 3 cut(s) 585, 1405, 1480
Bpu1102I GCTNAGC 1 cut(s) 2135
Bpu14I TTCGAA 1 cut(s) 1671
BpuEI CTTGAG 3 cut(s) 521, 656, 1329
BpuMI CCSGG 1 cut(s) 924
BsaAI YACGTR 2 cut(s) 1042, 1622
BsaBI GATNNNNATC 1 cut(s) 1379
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 3 cut(s) 155, 1287, 1288
BsaXI ACNNNNNCTCC 6 cut(s) 454, 484, 1530, 1560, 2175, 2205
Bsc4I CCNNNNNNNGG 4 cut(s) 680, 930, 1312, 1461
Bse118I RCCGGY 1 cut(s) 1855
Bse1I ACTGG 3 cut(s) 670, 1149, 1463
Bse3DI GCAATG 3 cut(s) 1074, 1787, 1877
Bse8I GATNNNNATC 1 cut(s) 1379
BseBI CCWGG 6 cut(s) 156, 675, 908, 947, 1020, 1289
BseDI CCNNGG 3 cut(s) 155, 1287, 1288
BseGI GGATG 4 cut(s) 619, 646, 670, 1513
BseJI GATNNNNATC 1 cut(s) 1379
BseLI CCNNNNNNNGG 4 cut(s) 680, 930, 1312, 1461
BseMI GCAATG 3 cut(s) 1074, 1787, 1877
BseMII CTCAG 4 cut(s) 771, 1586, 2149, 2488
BseNI ACTGG 3 cut(s) 670, 1149, 1463
BseRI GAGGAG 1 cut(s) 1612
BseSI GKGCMC 1 cut(s) 1586
BseXI GCAGC 6 cut(s) 33, 846, 1396, 2226, 2324, 2370
BseYI CCCAGC 1 cut(s) 1882
BsgI GTGCAG 3 cut(s) 122, 809, 2206
Bsh1236I CGCG 1 cut(s) 1728
BshFI GGCC 5 cut(s) 59, 531, 922, 1350, 1584
BshNI GGYRCC 2 cut(s) 355, 812
BsiHKAI GWGCWC 2 cut(s) 1452, 2369
BsiHKCI CYCGRG 2 cut(s) 119, 2368
BsiSI CCGG 2 cut(s) 924, 1856
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 4 cut(s) 680, 930, 1312, 1461
BsmAI GTCTC 5 cut(s) 379, 1712, 1960, 2432, 2477
BsmFI GGGAC 1 cut(s) 425
BsmI GAATGC 1 cut(s) 2042
BsnI GGCC 5 cut(s) 59, 531, 922, 1350, 1584
Bso31I GGTCTC 1 cut(s) 379
BsoBI CYCGRG 2 cut(s) 119, 2368
Bsp119I TTCGAA 1 cut(s) 1671
Bsp120I GGGCCC 1 cut(s) 1582
Bsp1286I GDGCHC 3 cut(s) 1452, 1586, 2369
Bsp1720I GCTNAGC 1 cut(s) 2135
BspACI CCGC 6 cut(s) 18, 578, 1224, 1412, 1921, 2498
BspANI GGCC 5 cut(s) 59, 531, 922, 1350, 1584
BspCNI CTCAG 4 cut(s) 772, 1587, 2148, 2487
BspFNI CGCG 1 cut(s) 1728
BspLI GGNNCC 5 cut(s) 270, 357, 814, 1584, 2023
BspMAI CTGCAG 1 cut(s) 354
BspMI ACCTGC 2 cut(s) 343, 2107
BspPI GGATC 4 cut(s) 201, 884, 1668, 2409
BspT104I TTCGAA 1 cut(s) 1671
BspT107I GGYRCC 2 cut(s) 355, 812
BspTI CTTAAG 1 cut(s) 254
BspTNI GGTCTC 1 cut(s) 379
BsrBI CCGCTC 1 cut(s) 1921
BsrDI GCAATG 3 cut(s) 1074, 1787, 1877
BsrFI RCCGGY 1 cut(s) 1855
BsrI ACTGG 3 cut(s) 670, 1149, 1463
BssAI RCCGGY 1 cut(s) 1855
BssECI CCNNGG 3 cut(s) 155, 1287, 1288
BssSI CACGAG 1 cut(s) 1483
Bst2BI CACGAG 1 cut(s) 1483
Bst2UI CCWGG 6 cut(s) 156, 675, 908, 947, 1020, 1289
Bst4CI ACNGT 3 cut(s) 148, 592, 2150
Bst6I CTCTTC 4 cut(s) 159, 1715, 1958, 2430
BstAFI CTTAAG 1 cut(s) 254
BstBAI YACGTR 2 cut(s) 1042, 1622
BstBI TTCGAA 1 cut(s) 1671
BstC8I GCNNGC 5 cut(s) 952, 1093, 1467, 1560, 2007
BstDEI CTNAG 5 cut(s) 780, 1550, 1595, 2135, 2474
BstEII GGTNACC 1 cut(s) 1520
BstENI CCTNNNNNAGG 2 cut(s) 1310, 1459
BstF5I GGATG 4 cut(s) 619, 646, 670, 1513
BstFNI CGCG 1 cut(s) 1728
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 2 cut(s) 247, 1728
BstMAI GTCTC 5 cut(s) 379, 1712, 1960, 2432, 2477
BstMWI GCNNNNNNNGC 2 cut(s) 23, 2533
BstNI CCWGG 6 cut(s) 156, 675, 908, 947, 1020, 1289
BstPI GGTNACC 1 cut(s) 1520
BstSCI CCNGG 7 cut(s) 154, 673, 906, 922, 945, 1018, 1287
BstSFI CTRYAG 4 cut(s) 27, 350, 1970, 2296
BstSLI GKGCMC 1 cut(s) 1586
BstUI CGCG 1 cut(s) 1728
BstV1I GCAGC 6 cut(s) 33, 846, 1396, 2226, 2324, 2370
BstV2I GAAGAC 4 cut(s) 149, 1067, 1141, 1609
BstX2I RGATCY 3 cut(s) 889, 1123, 2401
BstYI RGATCY 3 cut(s) 889, 1123, 2401
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 5 cut(s) 59, 531, 922, 1350, 1584
BtsCI GGATG 4 cut(s) 619, 646, 670, 1513
BtsI GCAGTG 2 cut(s) 2086, 2369
BtsIMutI CAGTG 6 cut(s) 153, 663, 777, 1142, 2086, 2369
BveI ACCTGC 2 cut(s) 343, 2107
Cac8I GCNNGC 5 cut(s) 952, 1093, 1467, 1560, 2007
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 2 cut(s) 247, 1728
Cfr10I RCCGGY 1 cut(s) 1855
Cfr13I GGNCC 6 cut(s) 904, 921, 1327, 1582, 1583, 2217
CseI GACGC 2 cut(s) 780, 2525
Csp6I GTAC 3 cut(s) 280, 569, 813
CviAII CATG 7 cut(s) 1215, 1324, 1428, 1541, 1990, 1999, 2356
CviQI GTAC 3 cut(s) 280, 569, 813
DdeI CTNAG 5 cut(s) 780, 1550, 1595, 2135, 2474
Eam1104I CTCTTC 4 cut(s) 159, 1715, 1958, 2430
EarI CTCTTC 4 cut(s) 159, 1715, 1958, 2430
Ecl136II GAGCTC 1 cut(s) 1450
Eco24I GRGCYC 2 cut(s) 1452, 1586
Eco31I GGTCTC 1 cut(s) 379
Eco32I GATATC 3 cut(s) 238, 1381, 1980
Eco47I GGWCC 3 cut(s) 904, 1327, 2217
Eco47III AGCGCT 1 cut(s) 246
Eco53kI GAGCTC 1 cut(s) 1450
Eco57I CTGAAG 3 cut(s) 161, 1112, 1881
Eco88I CYCGRG 2 cut(s) 119, 2368
Eco91I GGTNACC 1 cut(s) 1520
EcoICRI GAGCTC 1 cut(s) 1450
EcoNI CCTNNNNNAGG 2 cut(s) 1310, 1459
EcoO109I RGGNCCY 3 cut(s) 1582, 1583, 2217
EcoO65I GGTNACC 1 cut(s) 1520
EcoRII CCWGG 6 cut(s) 154, 673, 906, 945, 1018, 1287
EcoRV GATATC 3 cut(s) 238, 1381, 1980
EcoT22I ATGCAT 1 cut(s) 1789
EcoT38I GRGCYC 2 cut(s) 1452, 1586
FaeI CATG 7 cut(s) 1218, 1327, 1431, 1544, 1993, 2002, 2359
FalI AAGNNNNNCTT 2 cut(s) 1093, 1125
FaqI GGGAC 1 cut(s) 425
FatI CATG 7 cut(s) 1214, 1323, 1427, 1540, 1989, 1998, 2355
FbaI TGATCA 1 cut(s) 507
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 7 cut(s) 18, 47, 860, 1410, 2240, 2338, 2384
FokI GGATG 4 cut(s) 626, 653, 657, 1520
FriOI GRGCYC 2 cut(s) 1452, 1586
Fsp4HI GCNGC 7 cut(s) 18, 47, 860, 1410, 2240, 2338, 2384
FspBI CTAG 7 cut(s) 185, 533, 1221, 1685, 1757, 2189, 2519
GlaI GCGC 2 cut(s) 246, 1727
GluI GCNGC 7 cut(s) 18, 47, 860, 1410, 2240, 2338, 2384
GsaI CCCAGC 1 cut(s) 1886
GsuI CTGGAG 3 cut(s) 585, 1405, 1480
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 5 cut(s) 59, 531, 922, 1350, 1584
HapII CCGG 2 cut(s) 924, 1856
HgaI GACGC 2 cut(s) 780, 2525
HhaI GCGC 2 cut(s) 247, 1728
Hin1II CATG 7 cut(s) 1218, 1327, 1431, 1544, 1993, 2002, 2359
Hin6I GCGC 2 cut(s) 245, 1726
HinP1I GCGC 2 cut(s) 245, 1726
HincII GTYRAC 2 cut(s) 757, 2415
HindII GTYRAC 2 cut(s) 757, 2415
HindIII AAGCTT 1 cut(s) 2007
HinfI GANTC 7 cut(s) 323, 938, 968, 1072, 1357, 2373, 2390
HpaII CCGG 2 cut(s) 924, 1856
HphI GGTGA 7 cut(s) 215, 1009, 1304, 1444, 1532, 2134, 2339
Hpy166II GTNNAC 3 cut(s) 757, 1567, 2415
Hpy8I GTNNAC 3 cut(s) 757, 1567, 2415
Hpy99I CGWCG 1 cut(s) 761
HpyAV CCTTC 5 cut(s) 1347, 1489, 1678, 1816, 2063
HpyCH4III ACNGT 3 cut(s) 148, 592, 2150
HpyCH4IV ACGT 6 cut(s) 607, 759, 915, 1041, 1621, 2411
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 2533
HpyF3I CTNAG 5 cut(s) 780, 1550, 1595, 2135, 2474
HpySE526I ACGT 6 cut(s) 607, 759, 915, 1041, 1621, 2411
Hsp92II CATG 7 cut(s) 1218, 1327, 1431, 1544, 1993, 2002, 2359
HspAI GCGC 2 cut(s) 245, 1726
KpnI GGTACC 1 cut(s) 816
Ksp22I TGATCA 1 cut(s) 507
Lsp1109I GCAGC 6 cut(s) 33, 846, 1396, 2226, 2324, 2370
LweI GCATC 5 cut(s) 29, 753, 1054, 1273, 2148
MaeI CTAG 7 cut(s) 185, 533, 1221, 1685, 1757, 2189, 2519
MaeII ACGT 6 cut(s) 607, 759, 915, 1041, 1621, 2411
MaeIII GTNAC 9 cut(s) 258, 345, 661, 997, 1520, 1531, 1834, 1994, 2537
MbiI CCGCTC 1 cut(s) 1921
MfeI CAATTG 1 cut(s) 2290
MflI RGATCY 3 cut(s) 889, 1123, 2401
MhlI GDGCHC 3 cut(s) 1452, 1586, 2369
MlyI GAGTC 1 cut(s) 1081
MmeI TCCRAC 2 cut(s) 574, 1141
Mph1103I ATGCAT 1 cut(s) 1789
MroXI GAANNNNTTC 2 cut(s) 1125, 1677
MseI TTAA 6 cut(s) 255, 549, 957, 1239, 2213, 2304
MslI CAYNNNNRTG 3 cut(s) 1400, 1720, 2325
MspCI CTTAAG 1 cut(s) 254
MspI CCGG 2 cut(s) 924, 1856
MspR9I CCNGG 7 cut(s) 156, 675, 908, 924, 947, 1020, 1289
MunI CAATTG 1 cut(s) 2290
Mva1269I GAATGC 1 cut(s) 2042
MvaI CCWGG 6 cut(s) 156, 675, 908, 947, 1020, 1289
MvnI CGCG 1 cut(s) 1728
MwoI GCNNNNNNNGC 2 cut(s) 23, 2533
NciI CCSGG 1 cut(s) 924
NlaIII CATG 7 cut(s) 1218, 1327, 1431, 1544, 1993, 2002, 2359
NlaIV GGNNCC 5 cut(s) 270, 357, 814, 1584, 2023
NmuCI GTSAC 5 cut(s) 661, 997, 1520, 1834, 2537
NsiI ATGCAT 1 cut(s) 1789
NspV TTCGAA 1 cut(s) 1671
OliI CACNNNNGTG 1 cut(s) 2325
PaeR7I CTCGAG 2 cut(s) 119, 2368
PaqCI CACCTGC 1 cut(s) 343
PasI CCCWGGG 1 cut(s) 1288
PctI GAATGC 1 cut(s) 2042
PdmI GAANNNNTTC 2 cut(s) 1125, 1677
PfeI GAWTC 6 cut(s) 323, 938, 968, 1357, 2373, 2390
PflMI CCANNNNNTGG 1 cut(s) 680
PfoI TCCNGGA 1 cut(s) 906
PkrI GCNGC 7 cut(s) 19, 48, 861, 1411, 2241, 2339, 2385
PleI GAGTC 1 cut(s) 1080
PpsI GAGTC 1 cut(s) 1080
Ppu21I YACGTR 2 cut(s) 1042, 1622
PpuMI RGGWCCY 1 cut(s) 2217
PsiI TTATAA 2 cut(s) 392, 1613
Psp124BI GAGCTC 1 cut(s) 1452
Psp1406I AACGTT 2 cut(s) 915, 2411
Psp5II RGGWCCY 1 cut(s) 2217
Psp6I CCWGG 6 cut(s) 154, 673, 906, 945, 1018, 1287
PspEI GGTNACC 1 cut(s) 1520
PspFI CCCAGC 1 cut(s) 1882
PspGI CCWGG 6 cut(s) 154, 673, 906, 945, 1018, 1287
PspN4I GGNNCC 5 cut(s) 270, 357, 814, 1584, 2023
PspOMI GGGCCC 1 cut(s) 1582
PspPI GGNCC 6 cut(s) 904, 921, 1327, 1582, 1583, 2217
PspPPI RGGWCCY 1 cut(s) 2217
PstI CTGCAG 1 cut(s) 354
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 3 cut(s) 889, 1123, 2401
RsaI GTAC 3 cut(s) 281, 570, 814
RsaNI GTAC 3 cut(s) 280, 569, 813
RseI CAYNNNNRTG 3 cut(s) 1400, 1720, 2325
SacI GAGCTC 1 cut(s) 1452
SalI GTCGAC 1 cut(s) 755
SaqAI TTAA 6 cut(s) 255, 549, 957, 1239, 2213, 2304
SatI GCNGC 7 cut(s) 18, 47, 860, 1410, 2240, 2338, 2384
Sau96I GGNCC 6 cut(s) 904, 921, 1327, 1582, 1583, 2217
SchI GAGTC 1 cut(s) 1081
ScrFI CCNGG 7 cut(s) 156, 675, 908, 924, 947, 1020, 1289
SduI GDGCHC 3 cut(s) 1452, 1586, 2369
SfaNI GCATC 5 cut(s) 29, 753, 1054, 1273, 2148
SfcI CTRYAG 4 cut(s) 27, 350, 1970, 2296
Sfr274I CTCGAG 2 cut(s) 119, 2368
SfuI TTCGAA 1 cut(s) 1671
SinI GGWCC 3 cut(s) 904, 1327, 2217
SlaI CTCGAG 2 cut(s) 119, 2368
SmiMI CAYNNNNRTG 3 cut(s) 1400, 1720, 2325
SmlI CTYRAG 6 cut(s) 119, 254, 500, 635, 1344, 2368
SmoI CTYRAG 6 cut(s) 119, 254, 500, 635, 1344, 2368
SsiI CCGC 6 cut(s) 18, 578, 1224, 1412, 1921, 2498
SspI AATATT 1 cut(s) 1186
SspMI CTAG 7 cut(s) 185, 533, 1221, 1685, 1757, 2189, 2519
SstI GAGCTC 1 cut(s) 1452
StyD4I CCNGG 7 cut(s) 154, 673, 906, 922, 945, 1018, 1287
TaaI ACNGT 3 cut(s) 148, 592, 2150
TaiI ACGT 6 cut(s) 610, 762, 918, 1044, 1624, 2414
TaqI TCGA 8 cut(s) 90, 120, 171, 231, 405, 756, 1671, 2369
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 6 cut(s) 323, 938, 968, 1357, 2373, 2390
Tru1I TTAA 6 cut(s) 255, 549, 957, 1239, 2213, 2304
Tru9I TTAA 6 cut(s) 255, 549, 957, 1239, 2213, 2304
TscAI CASTG 6 cut(s) 153, 670, 784, 1149, 2086, 2369
TseFI GTSAC 5 cut(s) 661, 997, 1520, 1834, 2537
TseI GCWGC 6 cut(s) 46, 859, 1409, 2239, 2337, 2383
Tsp45I GTSAC 5 cut(s) 661, 997, 1520, 1834, 2537
TspDTI ATGAA 8 cut(s) 336, 630, 1312, 1326, 1416, 1881, 1978, 2448
TspGWI ACGGA 2 cut(s) 416, 1070
TspRI CASTG 6 cut(s) 153, 670, 784, 1149, 2086, 2369
Van91I CCANNNNNTGG 1 cut(s) 680
Vha464I CTTAAG 1 cut(s) 254
VpaK11BI GGWCC 3 cut(s) 904, 1327, 2217
XagI CCTNNNNNAGG 2 cut(s) 1310, 1459
XapI RAATTY 4 cut(s) 734, 1765, 1796, 2030
XbaI TCTAGA 1 cut(s) 184
XhoI CTCGAG 2 cut(s) 119, 2368
XmiI GTMKAC 1 cut(s) 756
XmnI GAANNNNTTC 2 cut(s) 1125, 1677
XspI CTAG 7 cut(s) 185, 533, 1221, 1685, 1757, 2189, 2519
Zsp2I ATGCAT 1 cut(s) 1789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.