Rw2G044120

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000039
Physical Location & Seq
Forward (+)
70811472 .. 70818625
7154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G044120.1

Sequence Viewer

Length: 2580 bp
ATGGATATTAATCATGTGGAGTTTTTCAGAGGTGCTGTTCTAGGAACAGCATTCAGTGCACTCTATGCTGGCGTTGAAGATCTGATTGCCAAGAACCACATGTTCAGAAATCTCTTCAAAAACATCAAATCCGGGCTAGACACTCTGAATCCACTCATGGCCGAGATGACTCAAGGTTTAGAAGCAGTACTGAAGGTTGAGGAAGAGCTTGTTCTAAAGTGCTCAAATGTTAGTCGGTGGGATCTCTGCGGGAAGTACAAATATGCCACCAAACTTCGTGCATGGGAAGAAGATCTTGAAAGACAGTTGCAGCTGCTGCAGGTGCAGGGAATCAGGGATGGGAAGAAGACCGCGGTGGTAGTAGAGAGCATAGAGGTTTCTGTTGGGACTGTGAAGGAGGGGGTCATCAAAATTTCTACTACTATGGAGGAGGTGAACCAACGCATGATGGAGAATTTTGTGATACAAGACCAATCTGCTGATAATAAAGCTTGGTGTGCAGTACCTGAATTTCCAGAGATTGTGGTTGGATTGGAAGCTCCTTTGAAGGATTTGAAGACAAAGCTCCTTGGGAATGATGGATTATCAATGCTTGTGCTCACTGCTCCCGGTGGATGTGGGAAAACTACCTTGGCAACAAACTTTTGCAAAGATCAGGAAGTCAAAGATAAATTCGGCAAAAATATCTTCTTTGTCACTGTTTCGAAGAAGTATAGCTTAAATTCTATTTGGCTGCAAACATTTTTGAAGGAAGAAGGAGAGAAACCTTCACTGTTGGTCTTGGATGATGTTTGGTCTGGATCAGAATCTCTTCTTGACAAATTTGAATGCAGAATGGCAAATATTAAGATTCTTGTGACATCAAGATCTGAATTTCCCGCGTTCAGTCCTCTATATCGTTTAAACATGTTGGATAATGACGATGCAATGAAACTGTTTCGTCATTCAGCATCATTGAATGGTAAGAGCTCTCATATTCCAGCACATCTTTTGAAGAAAGTGTCCAAGTTTTTGCAGATAGTTGAGCGTTGTAAGGGATTTCCACTTGCCATTACAGTGGTGGGCAGATCGCTTCGTGCACAACCTTTAGAGATGTGTCAAAACAGACTAAGTCAATGGTCTAAAGGTTCAGTTATTTTTTATTTTGAGACTGATTTACTTCTTTCCCTCCAAAGCAGCTTGGATGCTTTGGATAAGGAAAGGGCTATCATCAAAGAATGTTTCTTGGACCTTGGTTCATTTCCTGAAGACAAAGAAATCCCCGTTGATGTTCTCATTGATATATGGTCCGAGGCTATCATCAGAGAATGTTTCTTGGACCTTGGTTCATTTCCTGAAGACAAAGAAATCCCCGTTGATGCCCTCATTGATATATGGTCCGAGTTATATGAAATTGATGAAGACACTTCATGTATTGCAAACCTCTATGAACTCAACAATCGGAGTCTTGCTAATCTTATAGACGACAGGTACTCGAAGTTGGATGGGGAAGGATATTATGGTTCACACTATGTCACCCAACATGATGTGCTTAGGGAGCTGGCAATTTATCACTCGAAACTAGATTCGGTCGAGCATAGAGAGAGATTGATCATAGACATATGTGGAAGCAATCTTCCCAAGTGGTGGAGGGAACAAAAGTATCAAGTTATGAAAGCTCGCCTTCTATCTATATCATTAGGTTTTCTCCCCACACTTACATCCAATAACATTTTGTATGTAGATGGAGAGTCTTCAACTTCGAAATGGCCCAGCATGCATCTGCCAGAAACAGAGGTACTGCTCTTCAATCTTCGGGAGAGATGGCATTATGATATACCCAAATTTGTGGAGAAAATGGACAAATTGAAGGTACTAGTAATCACAAGTGAGTATGTCTGGGGTAGAGCTTCCTTGCGTAATTTTCCAGTACTAAGTCGGTTATCCAATCTAAAGAGAATCAGATTAGAGGGCATATGGATGAAATTCAAGAACTCCATACAAGTGAAGAGTCTACGGAAGTTGTCTTTGATCGAATGTAAAACCAAAATCGGTTCAGCTGAACTTTCCCATGCTTTTCCAAATCTAGAAGAGATATACATCAAAACATCAGTATTAACTCACTCGCCAAGTGATATAAAAAGTGTGACACTAATTCACGGGCTATGTGATCTGATTTCCCTAAAGAAGGTCACCCTCACAGGTGTTGTTGTACCTCTATTATGGGATTTGCCTGACGAGATTGGAAATTTGGTCAATTTGCAAGTATTGAGGCTGATGTCATGTCCTTGGCTTTCAAAGGTTCCAAACTCGATCAAAAACCTCAAGAAGTTGAACTTTCTTGGCATCTATGATTGCAGAAACTTTCGCGAGTTACCTGAATGCATTGGTGAAGTGAGGAGTTTAAGAACGATCGACTTGAGAAGTTGCTTGAGATTGAAGAATCTGCCTCGATCAGTATTGGATTTGGAGCAGTTAGAGGAGGTGATATGTAATGAAGATACAGAAAGCTTTTGGAAATCCCTGTTGCCCAGTTTCAGAGACAGAAACCAAGACATACGCATAGTGATTGTGCCGTCACCATGGTCACCATGGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

859

Amino Acids

98.22

Weight (kDa)

6.16

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 123 5.4e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 179 - 316 8.6e-11 NB-ARC domain
LRR_14 PF23598 714 - 778 2.6e-06 Leucine-rich repeat region
LRR_14 PF23598 767 - 829 1.2e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 310
Acc36I ACCTGC 1 cut(s) 310
AccB7I CCANNNNNTGG 1 cut(s) 1626
AccI GTMKAC 1 cut(s) 1993
AccII CGCG 3 cut(s) 353, 881, 2349
AciI CCGC 4 cut(s) 249, 351, 353, 879
AclWI GGATC 2 cut(s) 249, 808
AcoI YGGCCR 1 cut(s) 159
AcuI CTGAAG 3 cut(s) 212, 1266, 1356
AfaI GTAC 8 cut(s) 189, 257, 504, 1472, 1779, 1854, 1911, 2193
AfiI CCNNNNNNNGG 2 cut(s) 1626, 2167
AflIII ACRYGT 2 cut(s) 99, 906
AhlI ACTAGT 1 cut(s) 1855
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
Alw21I GWGCWC 5 cut(s) 61, 224, 600, 971, 1081
Alw26I GTCTC 2 cut(s) 1142, 2514
Alw44I GTGCAC 2 cut(s) 57, 1077
AlwI GGATC 2 cut(s) 249, 808
AlwNI CAGNNNCTG 2 cut(s) 316, 506
AoxI GGCC 2 cut(s) 159, 1748
ApaLI GTGCAC 2 cut(s) 57, 1077
ApeKI GCWGC 5 cut(s) 310, 313, 316, 733, 1176
AseI ATTAAT 1 cut(s) 9
Asp700I GAANNNNTTC 1 cut(s) 810
AspS9I GGNCC 5 cut(s) 1228, 1287, 1318, 1377, 1749
AsuC2I CCSGG 2 cut(s) 133, 609
AsuHPI GGTGA 7 cut(s) 445, 1507, 2164, 2381, 2476, 2550, 2559
AsuII TTCGAA 2 cut(s) 704, 1742
AvaII GGWCC 4 cut(s) 1228, 1287, 1318, 1377
BaeGI GKGCMC 2 cut(s) 61, 1081
BaeI ACNNNNGTAYC 2 cut(s) 1625, 1658
BanII GRGCYC 1 cut(s) 971
BbsI GAAGAC 6 cut(s) 353, 563, 1254, 1344, 1407, 1725
Bbv12I GWGCWC 5 cut(s) 61, 224, 600, 971, 1081
BbvI GCAGC 5 cut(s) 300, 303, 322, 720, 1188
BccI CCATC 6 cut(s) 332, 442, 572, 1478, 1718, 1797
BceAI ACGGC 1 cut(s) 2539
BclI TGATCA 1 cut(s) 1590
BcnI CCSGG 2 cut(s) 133, 609
BcoDI GTCTC 2 cut(s) 1142, 2514
BcuI ACTAGT 1 cut(s) 1855
BfaI CTAG 5 cut(s) 41, 137, 1562, 1856, 2066
BfmI CTRYAG 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 310
BglII AGATCT 3 cut(s) 79, 292, 866
BisI GCNGC 5 cut(s) 311, 314, 317, 734, 1177
BlsI GCNGC 5 cut(s) 312, 315, 318, 735, 1178
BmcAI AGTACT 2 cut(s) 189, 1911
Bme1390I CCNGG 2 cut(s) 133, 609
Bme18I GGWCC 4 cut(s) 1228, 1287, 1318, 1377
BmgT120I GGNCC 5 cut(s) 1228, 1287, 1318, 1377, 1749
BmiI GGNNCC 1 cut(s) 2283
BmrFI CCNGG 2 cut(s) 133, 609
BmrI ACTGGG 1 cut(s) 2505
BmsI GCATC 6 cut(s) 913, 959, 1174, 1348, 1768, 2334
BmuI ACTGGG 1 cut(s) 2505
BpiI GAAGAC 6 cut(s) 353, 563, 1254, 1344, 1407, 1725
Bpu10I CCTNAGC 1 cut(s) 1532
Bpu14I TTCGAA 2 cut(s) 704, 1742
BpuEI CTTGAG 4 cut(s) 156, 2288, 2419, 2431
BpuMI CCSGG 2 cut(s) 133, 609
BsaBI GATNNNNATC 3 cut(s) 9, 805, 2078
BsaJI CCNNGG 9 cut(s) 351, 568, 630, 1231, 1290, 1321, 2267, 2561, 2570
Bsc4I CCNNNNNNNGG 2 cut(s) 1626, 2167
Bse1I ACTGG 2 cut(s) 1907, 2511
Bse3DI GCAATG 1 cut(s) 933
Bse8I GATNNNNATC 3 cut(s) 9, 805, 2078
BseDI CCNNGG 9 cut(s) 351, 568, 630, 1231, 1290, 1321, 2267, 2561, 2570
BseGI GGATG 7 cut(s) 343, 620, 790, 1189, 1489, 1700, 1965
BseJI GATNNNNATC 3 cut(s) 9, 805, 2078
BseLI CCNNNNNNNGG 2 cut(s) 1626, 2167
BseMI GCAATG 1 cut(s) 933
BseNI ACTGG 2 cut(s) 1907, 2511
BseRI GAGGAG 3 cut(s) 443, 2392, 2474
BseSI GKGCMC 2 cut(s) 61, 1081
BseXI GCAGC 5 cut(s) 300, 303, 322, 720, 1188
BseYI CCCAGC 1 cut(s) 1751
BsgI GTGCAG 2 cut(s) 344, 519
Bsh1236I CGCG 3 cut(s) 353, 881, 2349
Bsh1285I CGRYCG 2 cut(s) 1572, 2394
BshFI GGCC 2 cut(s) 161, 1750
BsiEI CGRYCG 2 cut(s) 1572, 2394
BsiHKAI GWGCWC 5 cut(s) 61, 224, 600, 971, 1081
BsiSI CCGG 2 cut(s) 132, 609
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 1626, 2167
BsmAI GTCTC 2 cut(s) 1142, 2514
BsmFI GGGAC 1 cut(s) 400
BsmI GAATGC 3 cut(s) 50, 833, 2366
BsnI GGCC 2 cut(s) 161, 1750
Bsp119I TTCGAA 2 cut(s) 704, 1742
Bsp1286I GDGCHC 5 cut(s) 61, 224, 600, 971, 1081
Bsp19I CCATGG 2 cut(s) 2561, 2570
Bsp68I TCGCGA 1 cut(s) 2349
BspACI CCGC 4 cut(s) 249, 351, 353, 879
BspANI GGCC 2 cut(s) 161, 1750
BspFNI CGCG 3 cut(s) 353, 881, 2349
BspLI GGNNCC 1 cut(s) 2283
BspMAI CTGCAG 1 cut(s) 321
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 2 cut(s) 249, 808
BspQI GCTCTTC 2 cut(s) 198, 1790
BspT104I TTCGAA 2 cut(s) 704, 1742
BsrDI GCAATG 1 cut(s) 933
BsrI ACTGG 2 cut(s) 1907, 2511
BssECI CCNNGG 9 cut(s) 351, 568, 630, 1231, 1290, 1321, 2267, 2561, 2570
BssT1I CCWWGG 7 cut(s) 568, 630, 1231, 1321, 2267, 2561, 2570
Bst4CI ACNGT 6 cut(s) 306, 391, 700, 774, 936, 1057
Bst6I CTCTTC 6 cut(s) 119, 198, 816, 1790, 1982, 2064
BstAPI GCANNNNNTGC 3 cut(s) 56, 65, 316
BstBI TTCGAA 2 cut(s) 704, 1742
BstC8I GCNNGC 4 cut(s) 70, 1542, 1660, 1757
BstDEI CTNAG 3 cut(s) 1109, 1532, 1913
BstDSI CCRYGG 3 cut(s) 351, 2561, 2570
BstEII GGTNACC 2 cut(s) 2170, 2565
BstENI CCTNNNNNAGG 1 cut(s) 2165
BstF5I GGATG 7 cut(s) 343, 620, 790, 1189, 1489, 1700, 1965
BstFNI CGCG 3 cut(s) 353, 881, 2349
BstMAI GTCTC 2 cut(s) 1142, 2514
BstMCI CGRYCG 2 cut(s) 1572, 2394
BstMWI GCNNNNNNNGC 7 cut(s) 56, 65, 316, 322, 497, 1537, 1756
BstNSI RCATGY 3 cut(s) 103, 910, 1759
BstPI GGTNACC 2 cut(s) 2170, 2565
BstSCI CCNGG 2 cut(s) 131, 607
BstSFI CTRYAG 1 cut(s) 317
BstSLI GKGCMC 2 cut(s) 61, 1081
BstUI CGCG 3 cut(s) 353, 881, 2349
BstV1I GCAGC 5 cut(s) 300, 303, 322, 720, 1188
BstV2I GAAGAC 6 cut(s) 353, 563, 1254, 1344, 1407, 1725
BstX2I RGATCY 4 cut(s) 79, 241, 292, 866
BstXI CCANNNNNNTGG 2 cut(s) 1057, 1828
BstYI RGATCY 4 cut(s) 79, 241, 292, 866
BsuRI GGCC 2 cut(s) 161, 1750
BtgI CCRYGG 3 cut(s) 351, 2561, 2570
BtsCI GGATG 7 cut(s) 343, 620, 790, 1189, 1489, 1700, 1965
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 5 cut(s) 61, 600, 696, 770, 1062
BtuMI TCGCGA 1 cut(s) 2349
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 4 cut(s) 70, 1542, 1660, 1757
CaiI CAGNNNCTG 2 cut(s) 316, 506
Cfr13I GGNCC 5 cut(s) 1228, 1287, 1318, 1377, 1749
Cfr42I CCGCGG 1 cut(s) 354
Csp6I GTAC 8 cut(s) 188, 256, 503, 1471, 1778, 1853, 1910, 2192
CviQI GTAC 8 cut(s) 188, 256, 503, 1471, 1778, 1853, 1910, 2192
DdeI CTNAG 3 cut(s) 1109, 1532, 1913
DraI TTTAAA 1 cut(s) 903
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 6 cut(s) 119, 198, 816, 1790, 1982, 2064
EarI CTCTTC 6 cut(s) 119, 198, 816, 1790, 1982, 2064
Ecl136II GAGCTC 1 cut(s) 969
Eco130I CCWWGG 7 cut(s) 568, 630, 1231, 1321, 2267, 2561, 2570
Eco24I GRGCYC 1 cut(s) 971
Eco47I GGWCC 4 cut(s) 1228, 1287, 1318, 1377
Eco53kI GAGCTC 1 cut(s) 969
Eco57I CTGAAG 3 cut(s) 212, 1266, 1356
Eco91I GGTNACC 2 cut(s) 2170, 2565
EcoICRI GAGCTC 1 cut(s) 969
EcoNI CCTNNNNNAGG 1 cut(s) 2165
EcoO65I GGTNACC 2 cut(s) 2170, 2565
EcoT14I CCWWGG 7 cut(s) 568, 630, 1231, 1321, 2267, 2561, 2570
EcoT22I ATGCAT 2 cut(s) 1761, 2366
EcoT38I GRGCYC 1 cut(s) 971
ErhI CCWWGG 7 cut(s) 568, 630, 1231, 1321, 2267, 2561, 2570
FalI AAGNNNNNCTT 8 cut(s) 279, 311, 701, 733, 1647, 1679, 2300, 2332
FaqI GGGAC 1 cut(s) 400
FauI CCCGC 2 cut(s) 242, 886
FauNDI CATATG 2 cut(s) 1601, 1955
FbaI TGATCA 1 cut(s) 1590
FblI GTMKAC 1 cut(s) 1993
Fnu4HI GCNGC 5 cut(s) 311, 314, 317, 734, 1177
FokI GGATG 7 cut(s) 350, 627, 797, 1196, 1496, 1687, 1972
FriOI GRGCYC 1 cut(s) 971
Fsp4HI GCNGC 5 cut(s) 311, 314, 317, 734, 1177
FspBI CTAG 5 cut(s) 41, 137, 1562, 1856, 2066
GluI GCNGC 5 cut(s) 311, 314, 317, 734, 1177
GsaI CCCAGC 1 cut(s) 1755
HaeIII GGCC 2 cut(s) 161, 1750
HapII CCGG 2 cut(s) 132, 609
HindIII AAGCTT 2 cut(s) 489, 2488
HpaII CCGG 2 cut(s) 132, 609
HphI GGTGA 7 cut(s) 445, 1507, 2164, 2381, 2476, 2550, 2559
Hpy166II GTNNAC 5 cut(s) 59, 436, 1079, 1505, 1994
Hpy8I GTNNAC 5 cut(s) 59, 436, 1079, 1505, 1994
HpyCH4III ACNGT 6 cut(s) 306, 391, 700, 774, 936, 1057
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 65, 316, 322, 497, 1537, 1756
HpyF3I CTNAG 3 cut(s) 1109, 1532, 1913
Ksp22I TGATCA 1 cut(s) 1590
KspI CCGCGG 1 cut(s) 354
LguI GCTCTTC 2 cut(s) 198, 1790
LmnI GCTCC 5 cut(s) 544, 570, 610, 1537, 2449
Lsp1109I GCAGC 5 cut(s) 300, 303, 322, 720, 1188
LweI GCATC 6 cut(s) 913, 959, 1174, 1348, 1768, 2334
MaeI CTAG 5 cut(s) 41, 137, 1562, 1856, 2066
MaeIII GTNAC 8 cut(s) 694, 856, 1513, 2125, 2170, 2352, 2556, 2565
MflI RGATCY 4 cut(s) 79, 241, 292, 866
MhlI GDGCHC 5 cut(s) 61, 224, 600, 971, 1081
MlyI GAGTC 4 cut(s) 163, 1453, 1739, 1999
MmeI TCCRAC 3 cut(s) 508, 891, 1461
Mph1103I ATGCAT 2 cut(s) 1761, 2366
MroXI GAANNNNTTC 1 cut(s) 810
MseI TTAA 6 cut(s) 9, 719, 846, 902, 2096, 2384
MslI CAYNNNNRTG 3 cut(s) 1055, 1958, 1982
MspA1I CMGCKG 3 cut(s) 313, 353, 2039
MspI CCGG 2 cut(s) 132, 609
MspR9I CCNGG 2 cut(s) 133, 609
MssI GTTTAAAC 1 cut(s) 903
Mva1269I GAATGC 3 cut(s) 50, 833, 2366
MvnI CGCG 3 cut(s) 353, 881, 2349
MwoI GCNNNNNNNGC 7 cut(s) 56, 65, 316, 322, 497, 1537, 1756
NciI CCSGG 2 cut(s) 133, 609
NcoI CCATGG 2 cut(s) 2561, 2570
NdeI CATATG 2 cut(s) 1601, 1955
NlaIV GGNNCC 1 cut(s) 2283
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 7 cut(s) 694, 856, 1513, 2125, 2170, 2556, 2565
NruI TCGCGA 1 cut(s) 2349
NsiI ATGCAT 2 cut(s) 1761, 2366
NspI RCATGY 3 cut(s) 103, 910, 1759
NspV TTCGAA 2 cut(s) 704, 1742
PaeI GCATGC 1 cut(s) 1759
PaqCI CACCTGC 1 cut(s) 310
PciI ACATGT 2 cut(s) 99, 906
PciSI GCTCTTC 2 cut(s) 198, 1790
PctI GAATGC 3 cut(s) 50, 833, 2366
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 7 cut(s) 148, 330, 806, 850, 1565, 1938, 2422
PflFI GACNNNGTC 1 cut(s) 1110
PflMI CCANNNNNTGG 1 cut(s) 1626
PkrI GCNGC 5 cut(s) 312, 315, 318, 735, 1178
Ple19I CGATCG 1 cut(s) 2394
PleI GAGTC 4 cut(s) 163, 1452, 1738, 1998
PmeI GTTTAAAC 1 cut(s) 903
PpsI GAGTC 4 cut(s) 163, 1452, 1738, 1998
PscI ACATGT 2 cut(s) 99, 906
PshBI ATTAAT 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 971
PspEI GGTNACC 2 cut(s) 2170, 2565
PspFI CCCAGC 1 cut(s) 1751
PspN4I GGNNCC 1 cut(s) 2283
PspPI GGNCC 5 cut(s) 1228, 1287, 1318, 1377, 1749
PstI CTGCAG 1 cut(s) 321
PstNI CAGNNNCTG 2 cut(s) 316, 506
PsuI RGATCY 4 cut(s) 79, 241, 292, 866
PsyI GACNNNGTC 1 cut(s) 1110
PvuI CGATCG 1 cut(s) 2394
PvuII CAGCTG 2 cut(s) 313, 2039
RruI TCGCGA 1 cut(s) 2349
RsaI GTAC 8 cut(s) 189, 257, 504, 1472, 1779, 1854, 1911, 2193
RsaNI GTAC 8 cut(s) 188, 256, 503, 1471, 1778, 1853, 1910, 2192
RseI CAYNNNNRTG 3 cut(s) 1055, 1958, 1982
SacI GAGCTC 1 cut(s) 971
SacII CCGCGG 1 cut(s) 354
SapI GCTCTTC 2 cut(s) 198, 1790
SaqAI TTAA 6 cut(s) 9, 719, 846, 902, 2096, 2384
SatI GCNGC 5 cut(s) 311, 314, 317, 734, 1177
Sau96I GGNCC 5 cut(s) 1228, 1287, 1318, 1377, 1749
ScaI AGTACT 2 cut(s) 189, 1911
SchI GAGTC 4 cut(s) 163, 1453, 1739, 1999
ScrFI CCNGG 2 cut(s) 133, 609
SduI GDGCHC 5 cut(s) 61, 224, 600, 971, 1081
SfaNI GCATC 6 cut(s) 913, 959, 1174, 1348, 1768, 2334
SfcI CTRYAG 1 cut(s) 317
Sfr303I CCGCGG 1 cut(s) 354
SfuI TTCGAA 2 cut(s) 704, 1742
SgrBI CCGCGG 1 cut(s) 354
SinI GGWCC 4 cut(s) 1228, 1287, 1318, 1377
SmiMI CAYNNNNRTG 3 cut(s) 1055, 1958, 1982
SmlI CTYRAG 4 cut(s) 171, 2303, 2398, 2410
SmoI CTYRAG 4 cut(s) 171, 2303, 2398, 2410
SpeI ACTAGT 1 cut(s) 1855
SphI GCATGC 1 cut(s) 1759
SsiI CCGC 4 cut(s) 249, 351, 353, 879
SspI AATATT 1 cut(s) 844
SspMI CTAG 5 cut(s) 41, 137, 1562, 1856, 2066
SstI GAGCTC 1 cut(s) 971
StyD4I CCNGG 2 cut(s) 131, 607
StyI CCWWGG 7 cut(s) 568, 630, 1231, 1321, 2267, 2561, 2570
TaaI ACNGT 6 cut(s) 306, 391, 700, 774, 936, 1057
TaqI TCGA 9 cut(s) 704, 1475, 1556, 1572, 1742, 2013, 2291, 2394, 2431
TaqII GACCGA 1 cut(s) 1558
TatI WGTACW 3 cut(s) 187, 255, 1909
TfiI GAWTC 7 cut(s) 148, 330, 806, 850, 1565, 1938, 2422
Tru1I TTAA 6 cut(s) 9, 719, 846, 902, 2096, 2384
Tru9I TTAA 6 cut(s) 9, 719, 846, 902, 2096, 2384
TscAI CASTG 5 cut(s) 61, 607, 703, 777, 1062
TseFI GTSAC 7 cut(s) 694, 856, 1513, 2125, 2170, 2556, 2565
TseI GCWGC 5 cut(s) 310, 313, 316, 733, 1176
Tsp45I GTSAC 7 cut(s) 694, 856, 1513, 2125, 2170, 2556, 2565
TspGWI ACGGA 1 cut(s) 2011
TspRI CASTG 5 cut(s) 61, 607, 703, 777, 1062
Tth111I GACNNNGTC 1 cut(s) 1110
Van91I CCANNNNNTGG 1 cut(s) 1626
VneI GTGCAC 2 cut(s) 57, 1077
VpaK11BI GGWCC 4 cut(s) 1228, 1287, 1318, 1377
VspI ATTAAT 1 cut(s) 9
XagI CCTNNNNNAGG 1 cut(s) 2165
XbaI TCTAGA 1 cut(s) 2065
XceI RCATGY 3 cut(s) 103, 910, 1759
XcmI CCANNNNNNNNNTGG 2 cut(s) 1057, 2568
XmiI GTMKAC 1 cut(s) 1993
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 5 cut(s) 41, 137, 1562, 1856, 2066
ZrmI AGTACT 2 cut(s) 189, 1911
Zsp2I ATGCAT 2 cut(s) 1761, 2366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.