RchiOBHm_Chr1g0326451

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000039
Physical Location & Seq
Forward (+)
14992327 .. 14995980
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55608

Sequence Viewer

Length: 2571 bp
ATGGAGTTTATTGGAGTAGTATTTGTACAGCTGTTTAAGGTCGCTGTGGAACTTGCCAAATCCATGAAGTTTCAACCCATCCTAGGACAGATCGAATCCACTCTAGTCTCTTTGAAACCATTGATCGAAGAGATGGTAGGTCTAAACATGGGTTTGGAACTGAAAAGCCAGGCACTAGATAAGTTACAAGATGAAATCCTGGAGGGCCTAGTTCTTGTCCGCAAGTGCGTCAAGTGCTCCAAGGTCGACTTCCTCAAACGGATAAAATACAAGAAAAAACTTGAGAAATTGGATGAGTTCCTCAAAAGTCTGTTACAGGAACTCAAGGTAGAGGAAACGAGGAACGTAATGTTGAACTTACTTTTGACAAAGGAAACCTCCAAAAAAGTAGATGAAATGGCAAATGATGTGAAGGAGATTTTGCTTGGGACGGAGAAACATGGCGGCCTACTGGAAGAATTGCTACAGGGTGAGGAGGAGAGCTTGCTTTTGATCCGGAAAAACTACGACCAGATTGAACGATCGGAGAAAGAACAGAAGCAGTTCAGAGGGTTGGTCGAGTGGAGTATGGTCAATGTGCCTTCAAAGGATGACTTGCCTCCAATTAATTGGGATGAAGTCAAAGCTTCCACACTTCCCGTTGGATTTGATAAACCTATGGAGGAATTGAAGACAGATCTTCTCAAAAATGGGTTCCAAATGTTGGTTCTGACTGGTCTTCAAGGATCCGGGAAAACCACTTTGGCAAAACACTTTTGTGAAGATAAGGATGTCAAAGGTACATTCAAGGACAATATCTTCCTTGTCCATGTTTCGAAACAGCTCAGCTGTGGAGATATTGTAAGAAGGATTGTAAGAAAACTAAATAAGTACAAGAAAACCGAGTCACCAGACAACATAGATGACACAGTAATTGCAGTTGAGTGGCTGCAACAATTTCTGAATAAAGCAGGACAAAATCCTATACTGTTAGTCTTGGATGATGTTTGGCCTGGATCGGAATTCCTTCTTAAGAAGTTCAATGAATGCAAAATGTCGAATTGCAAGATTTTGGTGACATCAATTTACAATTTTCCAGGATTTTCTAGCCGATATTATTTTGAACCATTGGATGAATACAACGCAATCATTCTTCTTCAACATTCAGCATCCAGATATAAGCCCGCTTGTTTTACAGATGATTTTGCTAGAAAGGTAGTTGAGCGCTGCAGGGGGTTTCCACTCGCCATTGAGGAGGTCGGAAGATCACTAGGTGATCGAGCAACAGAATCCTGGGATAAAAGTCTAAGCAATTGGTATGAAAATTCGACTATTATTGACTCAGAGACTGATTTCTTTGGCTTTCTCCAAAGCAGCAAAGATACCTTGAATGAACAGATGGATACAGTAAAGGAATGTTTCATGGACCTTGGTTCATTTCCTGAAAATCAAAGAATACCTGTTGCTGCCCTCATTGAAATTTGGGCGGTGTTATATAAGCTAGAGGACGCTCCATCCATTTCGAACCTCTACGAGCTCACTAACCAGAGTTTGGCTAATCATATCTTCACAATGAAGGAGAAGATAGATGTCAATGGCTACTACAGTGAACACTTTGTTACCCAGCACTATCTGCTTAGACAGCTGGCCATATATGAAACGGGACAGGACCCAGTAGAACATAGAAAAAGGATGATTATAGATATCTGTGGAAACAATCTTCCCAAGTGGCTGAAGGAACATAAAGATCAACCAGTCAAGGCTCGCCTACTATCTATATCAACTGATAACAACTTCACAGAGAACTGGCACAACATGCAACTACCAGAGGTTGAAGTTCTAATTTTGAATTTTCATACAAAGGCCTATGCTTTACCTGGATTCGTGAAGACAATGGATAAATTGAAGGTTCTGATAGTCACAAATTATGGTTCCGAACCTGCTGAATTAAGACAATTTCATTTACTGGATAAGTTGTCAGATCTGAAGACAATCAGATTACAGAACATTTCATTAGCTTCCATCAGTAAGAACAGCCTTGCATTGAAGAGTCTACAGAAGATATCTTTATCCAGGTGCAGTATGGCTAAAGCTTTCAGTGCCCGTCGCATACAGATTTCAGATGCATTTCCAAATTTAGTGGAAATTAATATTGATTGTTGCAATGATTTGGAGAAATCGCTTGATAAGTTCTGTAGTCTTACTAGCCTAAAGAAACTCAGTATTACCAACTGTGATCTATCTATGTTATCGGAAGAGATTGGAGAGTTGAAAGATTTAGAATTGCTGAGGCTAAGGTCTTGTAGAGACTTGCACAAGTTGCCAGACTCAATTAGGAAACTCAAAATGCTAGACGTGCTTGACATATCTGATTGCTTGAGCCTAAGGACGTTGCCTGAAGACATTGGTGAAATGAAAAGTTTGAGAAAGCTCGACGTGAGACAGTGCAACGGACTGGAAAAGCTTCCTCAGTCAATTTTGAATCTTAAGCAGTTAGAGGAAGTGCTATGTGATGAAGTGACAGAAAATTTATGGGAGCCCTTCTTAGCCAAACTCAGCATAAAGGTGACCAAAGAAGATATTTCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

856

Amino Acids

98.2

Weight (kDa)

5.79

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 131 6.2e-15 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 217 - 354 3.6e-18 NB-ARC domain
AAA_16 PF13191 231 - 329 7.2e-06 AAA ATPase domain
AAA_22 PF13401 233 - 329 2.1e-06 AAA domain
nSTAND6 PF25201 233 - 313 5.5e-06 Novel STAND NTPase 6
LRR_14 PF23598 706 - 785 1.9e-08 Leucine-rich repeat region
LRR_14 PF23598 787 - 834 4.4e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1927
AccB7I CCANNNNNTGG 2 cut(s) 703, 1531
AccI GTMKAC 2 cut(s) 246, 2032
AccIII TCCGGA 1 cut(s) 495
AciI CCGC 4 cut(s) 220, 444, 1164, 1466
AclWI GGATC 4 cut(s) 487, 720, 733, 1003
AcoI YGGCCR 1 cut(s) 1626
AcsI RAATTY 6 cut(s) 1001, 1303, 1458, 1828, 2113, 2506
AcuI CTGAAG 3 cut(s) 1733, 1985, 2397
AdeI CACNNNGTG 1 cut(s) 1253
AfaI GTAC 3 cut(s) 27, 781, 872
AfeI AGCGCT 1 cut(s) 1205
AfiI CCNNNNNNNGG 3 cut(s) 83, 703, 1531
AflII CTTAAG 2 cut(s) 1010, 2465
AjiI CACGTC 2 cut(s) 2335, 2416
AjnI CCWGG 7 cut(s) 168, 198, 991, 1075, 1271, 1855, 2051
AjuI GAANNNNNNNTTGG 6 cut(s) 233, 265, 690, 722, 725, 757
AleI CACNNNNGTG 1 cut(s) 756
Alw21I GWGCWC 2 cut(s) 239, 1518
Alw26I GTCTC 4 cut(s) 112, 1319, 2280, 2413
AlwI GGATC 4 cut(s) 487, 720, 733, 1003
AlwNI CAGNNNCTG 1 cut(s) 1328
Aor13HI TCCGGA 1 cut(s) 495
Aor51HI AGCGCT 1 cut(s) 1205
AoxI GGCC 5 cut(s) 205, 445, 989, 1626, 1842
ApeKI GCWGC 4 cut(s) 928, 1206, 1353, 1445
ApoI RAATTY 6 cut(s) 1001, 1303, 1458, 1828, 2113, 2506
AseI ATTAAT 2 cut(s) 606, 2127
Asp700I GAANNNNTTC 3 cut(s) 542, 1005, 2442
AspA2I CCTAGG 1 cut(s) 82
AspLEI GCGC 1 cut(s) 1206
AspS9I GGNCC 3 cut(s) 205, 1405, 1648
AsuC2I CCSGG 1 cut(s) 730
AsuHPI GGTGA 6 cut(s) 482, 879, 1066, 1265, 2399, 2557
AsuII TTCGAA 2 cut(s) 815, 1502
AvaII GGWCC 2 cut(s) 1405, 1648
AvrII CCTAGG 1 cut(s) 82
AxyI CCTNAGG 1 cut(s) 2363
BaeGI GKGCMC 1 cut(s) 2083
BalI TGGCCA 1 cut(s) 1628
BamHI GGATCC 1 cut(s) 725
BanII GRGCYC 2 cut(s) 1518, 2520
BarI GAAGNNNNNNTAC 2 cut(s) 447, 479
BbsI GAAGAC 5 cut(s) 677, 710, 1874, 1973, 2385
Bbv12I GWGCWC 2 cut(s) 239, 1518
BbvCI CCTCAGC 1 cut(s) 2267
BbvI GCAGC 4 cut(s) 915, 1193, 1365, 1432
BccI CCATC 5 cut(s) 86, 127, 1372, 1501, 2009
BciT130I CCWGG 7 cut(s) 170, 200, 993, 1077, 1273, 1857, 2053
BciVI GTATCC 1 cut(s) 1375
BcnI CCSGG 1 cut(s) 730
BcoDI GTCTC 4 cut(s) 112, 1319, 2280, 2413
BfmI CTRYAG 5 cut(s) 464, 1207, 1582, 2033, 2173
BfoI RGCGCY 1 cut(s) 1207
BfrI CTTAAG 2 cut(s) 1010, 2465
BfuAI ACCTGC 1 cut(s) 1927
BfuI GTATCC 1 cut(s) 1375
BglII AGATCT 2 cut(s) 676, 1960
BisI GCNGC 5 cut(s) 445, 929, 1207, 1354, 1446
BlnI CCTAGG 1 cut(s) 82
BlpI GCTNAGC 1 cut(s) 824
BlsI GCNGC 5 cut(s) 446, 930, 1208, 1355, 1447
Bme1390I CCNGG 8 cut(s) 170, 200, 730, 993, 1077, 1273, 1857, 2053
Bme18I GGWCC 2 cut(s) 1405, 1648
BmgBI CACGTC 2 cut(s) 2335, 2416
BmgT120I GGNCC 3 cut(s) 205, 1405, 1648
BmiI GGNNCC 5 cut(s) 695, 727, 1650, 1912, 2517
BmrFI CCNGG 8 cut(s) 170, 200, 730, 993, 1077, 1273, 1857, 2053
BmrI ACTGGG 1 cut(s) 1646
BmsI GCATC 2 cut(s) 1157, 2092
BmuI ACTGGG 1 cut(s) 1646
BpiI GAAGAC 5 cut(s) 677, 710, 1874, 1973, 2385
BpmI CTGGAG 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 2267, 2273
Bpu1102I GCTNAGC 1 cut(s) 824
Bpu14I TTCGAA 2 cut(s) 815, 1502
BpuEI CTTGAG 3 cut(s) 302, 308, 2377
BpuMI CCSGG 1 cut(s) 730
BsaJI CCNNGG 4 cut(s) 82, 240, 1272, 1408
BsaWI WCCGGW 1 cut(s) 495
BsaXI ACNNNNNCTCC 2 cut(s) 825, 855
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 703, 1531
Bse1I ACTGG 7 cut(s) 456, 718, 1652, 1733, 1790, 1950, 2439
Bse21I CCTNAGG 1 cut(s) 2363
Bse3DI GCAATG 1 cut(s) 2149
BseAI TCCGGA 1 cut(s) 495
BseBI CCWGG 7 cut(s) 170, 200, 993, 1077, 1273, 1857, 2053
BseDI CCNNGG 4 cut(s) 82, 240, 1272, 1408
BseLI CCNNNNNNNGG 3 cut(s) 83, 703, 1531
BseMI GCAATG 1 cut(s) 2149
BseMII CTCAG 6 cut(s) 838, 1335, 2212, 2258, 2462, 2548
BseNI ACTGG 7 cut(s) 456, 718, 1652, 1733, 1790, 1950, 2439
BseRI GAGGAG 3 cut(s) 488, 491, 1247
BseSI GKGCMC 1 cut(s) 2083
BseXI GCAGC 4 cut(s) 915, 1193, 1365, 1432
BseYI CCCAGC 1 cut(s) 1602
BsgI GTGCAG 1 cut(s) 2077
Bsh1285I CGRYCG 1 cut(s) 524
BshFI GGCC 5 cut(s) 207, 447, 991, 1628, 1844
BsiEI CGRYCG 1 cut(s) 524
BsiHKAI GWGCWC 2 cut(s) 239, 1518
BsiSI CCGG 2 cut(s) 496, 729
BslFI GGGAC 2 cut(s) 442, 1656
BslI CCNNNNNNNGG 3 cut(s) 83, 703, 1531
BsmAI GTCTC 4 cut(s) 112, 1319, 2280, 2413
BsmFI GGGAC 2 cut(s) 442, 1656
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 5 cut(s) 207, 447, 991, 1628, 1844
Bsp119I TTCGAA 2 cut(s) 815, 1502
Bsp1286I GDGCHC 4 cut(s) 239, 1518, 2083, 2520
Bsp13I TCCGGA 1 cut(s) 495
Bsp1407I TGTACA 1 cut(s) 25
Bsp1720I GCTNAGC 1 cut(s) 824
BspACI CCGC 4 cut(s) 220, 444, 1164, 1466
BspANI GGCC 5 cut(s) 207, 447, 991, 1628, 1844
BspCNI CTCAG 6 cut(s) 837, 1334, 2211, 2259, 2461, 2547
BspEI TCCGGA 1 cut(s) 495
BspLI GGNNCC 5 cut(s) 695, 727, 1650, 1912, 2517
BspMAI CTGCAG 1 cut(s) 1211
BspMI ACCTGC 1 cut(s) 1927
BspPI GGATC 4 cut(s) 487, 720, 733, 1003
BspT104I TTCGAA 2 cut(s) 815, 1502
BspTI CTTAAG 2 cut(s) 1010, 2465
BsrDI GCAATG 1 cut(s) 2149
BsrGI TGTACA 1 cut(s) 25
BsrI ACTGG 7 cut(s) 456, 718, 1652, 1733, 1790, 1950, 2439
BssECI CCNNGG 4 cut(s) 82, 240, 1272, 1408
BssT1I CCWWGG 3 cut(s) 82, 240, 1408
Bst2UI CCWGG 7 cut(s) 170, 200, 993, 1077, 1273, 1857, 2053
Bst4CI ACNGT 6 cut(s) 910, 969, 1387, 1586, 2213, 2424
Bst6I CTCTTC 3 cut(s) 123, 2021, 2229
BstAFI CTTAAG 2 cut(s) 1010, 2465
BstAPI GCANNNNNTGC 3 cut(s) 1612, 1795, 2299
BstAUI TGTACA 1 cut(s) 25
BstBI TTCGAA 2 cut(s) 815, 1502
BstC8I GCNNGC 4 cut(s) 485, 1164, 1626, 1744
BstEII GGTNACC 1 cut(s) 2545
BstH2I RGCGCY 1 cut(s) 1207
BstHHI GCGC 1 cut(s) 1206
BstMAI GTCTC 4 cut(s) 112, 1319, 2280, 2413
BstMCI CGRYCG 1 cut(s) 524
BstMWI GCNNNNNNNGC 7 cut(s) 234, 1612, 1621, 1795, 2078, 2299, 2335
BstNI CCWGG 7 cut(s) 170, 200, 993, 1077, 1273, 1857, 2053
BstNSI RCATGY 1 cut(s) 1798
BstPI GGTNACC 1 cut(s) 2545
BstSCI CCNGG 8 cut(s) 168, 198, 728, 991, 1075, 1271, 1855, 2051
BstSFI CTRYAG 5 cut(s) 464, 1207, 1582, 2033, 2173
BstSLI GKGCMC 1 cut(s) 2083
BstV1I GCAGC 4 cut(s) 915, 1193, 1365, 1432
BstV2I GAAGAC 5 cut(s) 677, 710, 1874, 1973, 2385
BstX2I RGATCY 3 cut(s) 676, 725, 1960
BstXI CCANNNNNNTGG 1 cut(s) 609
BstYI RGATCY 3 cut(s) 676, 725, 1960
Bsu36I CCTNAGG 1 cut(s) 2363
BsuI GTATCC 1 cut(s) 1375
BsuRI GGCC 5 cut(s) 207, 447, 991, 1628, 1844
BtrI CACGTC 2 cut(s) 2335, 2416
BtsIMutI CAGTG 3 cut(s) 1591, 2083, 2429
BveI ACCTGC 1 cut(s) 1927
Cac8I GCNNGC 4 cut(s) 485, 1164, 1626, 1744
CaiI CAGNNNCTG 1 cut(s) 1328
CfoI GCGC 1 cut(s) 1206
Cfr13I GGNCC 3 cut(s) 205, 1405, 1648
CseI GACGC 2 cut(s) 217, 1496
Csp6I GTAC 3 cut(s) 26, 780, 871
CspCI CAANNNNNGTGG 2 cut(s) 2100, 2135
CviAII CATG 6 cut(s) 64, 148, 440, 809, 1402, 1795
CviQI GTAC 3 cut(s) 26, 780, 871
DraIII CACNNNGTG 1 cut(s) 1253
EaeI YGGCCR 1 cut(s) 1626
Eam1104I CTCTTC 3 cut(s) 123, 2021, 2229
EarI CTCTTC 3 cut(s) 123, 2021, 2229
Ecl136II GAGCTC 1 cut(s) 1516
Eco130I CCWWGG 3 cut(s) 82, 240, 1408
Eco147I AGGCCT 1 cut(s) 1844
Eco24I GRGCYC 2 cut(s) 1518, 2520
Eco32I GATATC 2 cut(s) 1684, 2043
Eco47I GGWCC 2 cut(s) 1405, 1648
Eco47III AGCGCT 1 cut(s) 1205
Eco53kI GAGCTC 1 cut(s) 1516
Eco57I CTGAAG 3 cut(s) 1733, 1985, 2397
Eco81I CCTNAGG 1 cut(s) 2363
Eco91I GGTNACC 1 cut(s) 2545
EcoICRI GAGCTC 1 cut(s) 1516
EcoO109I RGGNCCY 2 cut(s) 205, 1648
EcoO65I GGTNACC 1 cut(s) 2545
EcoRI GAATTC 1 cut(s) 1001
EcoRII CCWGG 7 cut(s) 168, 198, 991, 1075, 1271, 1855, 2051
EcoRV GATATC 2 cut(s) 1684, 2043
EcoT14I CCWWGG 3 cut(s) 82, 240, 1408
EcoT22I ATGCAT 1 cut(s) 2107
EcoT38I GRGCYC 2 cut(s) 1518, 2520
ErhI CCWWGG 3 cut(s) 82, 240, 1408
FaeI CATG 6 cut(s) 67, 151, 443, 812, 1405, 1798
FalI AAGNNNNNCTT 4 cut(s) 233, 265, 578, 610
FaqI GGGAC 2 cut(s) 442, 1656
FatI CATG 6 cut(s) 63, 147, 439, 808, 1401, 1794
FauI CCCGC 1 cut(s) 1171
FblI GTMKAC 2 cut(s) 246, 2032
Fnu4HI GCNGC 5 cut(s) 445, 929, 1207, 1354, 1446
FriOI GRGCYC 2 cut(s) 1518, 2520
Fsp4HI GCNGC 5 cut(s) 445, 929, 1207, 1354, 1446
GlaI GCGC 1 cut(s) 1205
GluI GCNGC 5 cut(s) 445, 929, 1207, 1354, 1446
GsaI CCCAGC 1 cut(s) 1606
GsuI CTGGAG 1 cut(s) 221
HaeII RGCGCY 1 cut(s) 1207
HaeIII GGCC 5 cut(s) 207, 447, 991, 1628, 1844
HapII CCGG 2 cut(s) 496, 729
HgaI GACGC 2 cut(s) 217, 1496
HhaI GCGC 1 cut(s) 1206
Hin1II CATG 6 cut(s) 67, 151, 443, 812, 1405, 1798
Hin6I GCGC 1 cut(s) 1204
HinP1I GCGC 1 cut(s) 1204
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 3 cut(s) 624, 2070, 2441
HinfI GANTC 8 cut(s) 95, 884, 1268, 1319, 1860, 2029, 2306, 2461
HpaII CCGG 2 cut(s) 496, 729
HphI GGTGA 6 cut(s) 482, 879, 1066, 1265, 2399, 2557
Hpy166II GTNNAC 3 cut(s) 247, 1589, 2033
Hpy188III TCNNGA 4 cut(s) 496, 1152, 1421, 1864
Hpy8I GTNNAC 3 cut(s) 247, 1589, 2033
Hpy99I CGWCG 2 cut(s) 2088, 2417
HpyAV CCTTC 8 cut(s) 406, 591, 840, 1016, 1549, 1708, 1879, 2530
HpyCH4III ACNGT 6 cut(s) 910, 969, 1387, 1586, 2213, 2424
HpyCH4IV ACGT 4 cut(s) 345, 2334, 2369, 2415
HpyF10VI GCNNNNNNNGC 7 cut(s) 234, 1612, 1621, 1795, 2078, 2299, 2335
HpySE526I ACGT 4 cut(s) 345, 2334, 2369, 2415
Hsp92II CATG 6 cut(s) 67, 151, 443, 812, 1405, 1798
HspAI GCGC 1 cut(s) 1204
Kpn2I TCCGGA 1 cut(s) 495
LmnI GCTCC 3 cut(s) 242, 1495, 2515
Lsp1109I GCAGC 4 cut(s) 915, 1193, 1365, 1432
LweI GCATC 2 cut(s) 1157, 2092
MaeII ACGT 4 cut(s) 345, 2334, 2369, 2415
MaeIII GTNAC 8 cut(s) 183, 312, 885, 1054, 1597, 1897, 2497, 2545
MfeI CAATTG 1 cut(s) 1291
MflI RGATCY 3 cut(s) 676, 725, 1960
MhlI GDGCHC 4 cut(s) 239, 1518, 2083, 2520
MlsI TGGCCA 1 cut(s) 1628
MluNI TGGCCA 1 cut(s) 1628
MlyI GAGTC 4 cut(s) 893, 1313, 2038, 2300
MmeI TCCRAC 2 cut(s) 622, 1219
Mox20I TGGCCA 1 cut(s) 1628
Mph1103I ATGCAT 1 cut(s) 2107
MroI TCCGGA 1 cut(s) 495
MroXI GAANNNNTTC 3 cut(s) 542, 1005, 2442
MscI TGGCCA 1 cut(s) 1628
MseI TTAA 6 cut(s) 36, 606, 1011, 1928, 2127, 2466
MslI CAYNNNNRTG 2 cut(s) 756, 2543
Msp20I TGGCCA 1 cut(s) 1628
MspA1I CMGCKG 3 cut(s) 31, 828, 1624
MspCI CTTAAG 2 cut(s) 1010, 2465
MspI CCGG 2 cut(s) 496, 729
MspR9I CCNGG 8 cut(s) 170, 200, 730, 993, 1077, 1273, 1857, 2053
MunI CAATTG 1 cut(s) 1291
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 7 cut(s) 170, 200, 993, 1077, 1273, 1857, 2053
MwoI GCNNNNNNNGC 7 cut(s) 234, 1612, 1621, 1795, 2078, 2299, 2335
NciI CCSGG 1 cut(s) 730
NlaIII CATG 6 cut(s) 67, 151, 443, 812, 1405, 1798
NlaIV GGNNCC 5 cut(s) 695, 727, 1650, 1912, 2517
NmuCI GTSAC 5 cut(s) 885, 1054, 1897, 2497, 2545
NsiI ATGCAT 1 cut(s) 2107
NspI RCATGY 1 cut(s) 1798
NspV TTCGAA 2 cut(s) 815, 1502
OliI CACNNNNGTG 1 cut(s) 756
PceI AGGCCT 1 cut(s) 1844
PctI GAATGC 1 cut(s) 1031
PdmI GAANNNNTTC 3 cut(s) 542, 1005, 2442
PfeI GAWTC 4 cut(s) 95, 1268, 1860, 2461
PflMI CCANNNNNTGG 2 cut(s) 703, 1531
PfoI TCCNGGA 3 cut(s) 198, 728, 1075
PkrI GCNGC 5 cut(s) 446, 930, 1208, 1355, 1447
Ple19I CGATCG 1 cut(s) 524
PleI GAGTC 4 cut(s) 892, 1313, 2037, 2300
PpsI GAGTC 4 cut(s) 892, 1313, 2037, 2300
PpuMI RGGWCCY 1 cut(s) 1648
PshBI ATTAAT 2 cut(s) 606, 2127
Psp124BI GAGCTC 1 cut(s) 1518
Psp5II RGGWCCY 1 cut(s) 1648
Psp6I CCWGG 7 cut(s) 168, 198, 991, 1075, 1271, 1855, 2051
PspEI GGTNACC 1 cut(s) 2545
PspFI CCCAGC 1 cut(s) 1602
PspGI CCWGG 7 cut(s) 168, 198, 991, 1075, 1271, 1855, 2051
PspN4I GGNNCC 5 cut(s) 695, 727, 1650, 1912, 2517
PspPI GGNCC 3 cut(s) 205, 1405, 1648
PspPPI RGGWCCY 1 cut(s) 1648
PstI CTGCAG 1 cut(s) 1211
PstNI CAGNNNCTG 1 cut(s) 1328
PsuI RGATCY 3 cut(s) 676, 725, 1960
PvuI CGATCG 1 cut(s) 524
PvuII CAGCTG 3 cut(s) 31, 828, 1624
RsaI GTAC 3 cut(s) 27, 781, 872
RsaNI GTAC 3 cut(s) 26, 780, 871
RseI CAYNNNNRTG 2 cut(s) 756, 2543
SacI GAGCTC 1 cut(s) 1518
SalI GTCGAC 1 cut(s) 245
SaqAI TTAA 6 cut(s) 36, 606, 1011, 1928, 2127, 2466
SatI GCNGC 5 cut(s) 445, 929, 1207, 1354, 1446
Sau96I GGNCC 3 cut(s) 205, 1405, 1648
SchI GAGTC 4 cut(s) 893, 1313, 2038, 2300
ScrFI CCNGG 8 cut(s) 170, 200, 730, 993, 1077, 1273, 1857, 2053
SduI GDGCHC 4 cut(s) 239, 1518, 2083, 2520
SfaNI GCATC 2 cut(s) 1157, 2092
SfcI CTRYAG 5 cut(s) 464, 1207, 1582, 2033, 2173
SfuI TTCGAA 2 cut(s) 815, 1502
SinI GGWCC 2 cut(s) 1405, 1648
SmiMI CAYNNNNRTG 2 cut(s) 756, 2543
SmlI CTYRAG 5 cut(s) 281, 323, 1010, 2356, 2465
SmoI CTYRAG 5 cut(s) 281, 323, 1010, 2356, 2465
SseBI AGGCCT 1 cut(s) 1844
SsiI CCGC 4 cut(s) 220, 444, 1164, 1466
SspI AATATT 1 cut(s) 2131
SstI GAGCTC 1 cut(s) 1518
StuI AGGCCT 1 cut(s) 1844
StyD4I CCNGG 8 cut(s) 168, 198, 728, 991, 1075, 1271, 1855, 2051
StyI CCWWGG 3 cut(s) 82, 240, 1408
TaaI ACNGT 6 cut(s) 910, 969, 1387, 1586, 2213, 2424
TaiI ACGT 4 cut(s) 348, 2337, 2372, 2418
TatI WGTACW 2 cut(s) 25, 870
TauI GCSGC 1 cut(s) 447
TfiI GAWTC 4 cut(s) 95, 1268, 1860, 2461
Tru1I TTAA 6 cut(s) 36, 606, 1011, 1928, 2127, 2466
Tru9I TTAA 6 cut(s) 36, 606, 1011, 1928, 2127, 2466
TscAI CASTG 3 cut(s) 1591, 2083, 2429
TseFI GTSAC 5 cut(s) 885, 1054, 1897, 2497, 2545
TseI GCWGC 4 cut(s) 928, 1206, 1353, 1445
Tsp45I GTSAC 5 cut(s) 885, 1054, 1897, 2497, 2545
TspGWI ACGGA 3 cut(s) 274, 446, 2445
TspRI CASTG 3 cut(s) 1591, 2083, 2429
Van91I CCANNNNNTGG 2 cut(s) 703, 1531
Vha464I CTTAAG 2 cut(s) 1010, 2465
VpaK11BI GGWCC 2 cut(s) 1405, 1648
VspI ATTAAT 2 cut(s) 606, 2127
XapI RAATTY 6 cut(s) 1001, 1303, 1458, 1828, 2113, 2506
XceI RCATGY 1 cut(s) 1798
XcmI CCANNNNNNNNNTGG 1 cut(s) 2059
XmaJI CCTAGG 1 cut(s) 82
XmiI GTMKAC 2 cut(s) 246, 2032
XmnI GAANNNNTTC 3 cut(s) 542, 1005, 2442
Zsp2I ATGCAT 1 cut(s) 2107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.