Rh2DG174900

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000039
Physical Location & Seq
Forward (+)
15377626 .. 15382228
4603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG174900.1

Sequence Viewer

Length: 2817 bp
ATGGAAACCGGAAAAGAAAGAAGCAAGCAGGGTGGCTCTCTCCAGTTCAGGTGTTATCAAATGAAGAAGAAGGTTCTTCTCGAGTGGTTGGCTCGGTCGCTCCGTCAGTTTTACATGTGCCTTTTACAGTCAATGGCCTCCAACTTGGAGCTTGCTGGGTCAGTCAAAGTCAATGGATTCCCAATTGGAAACTCGGAGCCTCACATTGAACATGTCCGTCCCCCTTTACAAGCTTTTTATTCGGGTCTTTGCATCACTGGACTTCATCAATTACCAAGATCAAGAATGGCGGACCTCGCTGCAGGTGGTGCTATGGGAGTGCCGTTTACTATACTGTATGAAGTGATTAAGGAAGTGGTGATCAAGACTTCGATGTTTAGACCTCTCCTTGAAGAGCTGAAGTCAAAGATAGAAGCTTTGAATCCACTGATTGCTCAGATGGACAGCTGGAATAAGGTGTTGGATCGTCCAGAGAAGGAACTTAGAGAATTTAAAGTACAAATGGAGAAGGGCCCTGAGCTTATTACCAAGTGCGCCAATCTTAGCAAGTGGAAAAGCTACAAAAAGTATAAATACAGCAACCAACTTCTTGAATTGCAGAACTCTCTTCAGAATCTGCTGTTTATACTGCAATGGCAACAAGCAAGGGATGTGAAGGAGACCTTGGTTGGAGTAAAGAATGTAGAGACGGTGGTGCAGCGAGTGGATGTGAGTGTTGCGGAATTCAAAGAAGAAGGGATGAGTGTCATGAAGGTGACCTTGGATGAGGTAAGAAATTTAGGGACGATGGTCCGGCGGATTGATAAGAAAGGTGAGGTACAGAACCCGTTAGATTGTGAAGTACCTGAACCTCCATTGGTTACAGTTGGATTGTATGTGCCTTTGAGGGAATTGAAGATGAAGCTTCTTAAGAATGATGATAATGTATCAATGCTTGTGCTAACTGCTCCTGGAGGCTGCGGGAAAACCACATTGGCCACAAAGTTTTGTCAAGATGAGGAAGTCAAAGATAGATTCAAGAACAATATCTTCTTCATCACTGTTACAAAGAAGTCAACCTTCAACCATATTGTACAAGGACTACATCAGCGTAAGAGTACCGACGTACCTATGTTCCAAGATGAAGTAACTGCTGTTAAGTGGCTGCGGAAATTTCTAATGGAAGAAGGACAGAACCCTGTACTGTTGGTCCTGGATGATGTTTGGTCTGAATCAAAATATATTCTTGAAAAATTTGATGAAGTGAAAATGCCAAATTTCAAGATTCTGGTGACATCAAGATCACAGTTTCCAGAATATGGCTCAATTTATCATCTAAAGTCATTGAATGATACAGATGCAATGACTCTTTTCCGCCATTCAGCATCCCTGGGAGATGGTCAATGGAATAGTCTTGCTAGAGAGATTGTAGCGCCTTGTAAGGGATATCCACTTGCAATTAAAGTGGTTGGAAAATCACTTCGCGGAAAACCGATTGAGTTCTGGCGCAAAAGAGCATTGGAGTGGTCTAAAGGTGATTCTATTATTGATTCCGAGACTGAAGTGCTTCTTCGCCTTCAAACTAGCTTAGATGCTTTGGATGAAAAAGAAGCTCTCATCAAAGAATGTTTTTTAGACCTTGGTTCATTTCCCCAAGACCAAAGAATTCCAGCAGGTGCTCTGATTGATATGTGGGCAGGGTTATATGAAGATCTCGATAAAGATTTTCTGGCCATTACAAACATGTATGAGCTTACCACCCGAAGTTTGGCTGATCTTGTAGTCACAAGGAAGGAGACAGTGGAGGATGATGACTACTACAGTGAACACTTTGTCACCCAACATGATTTGCTCAGACAGCTCGCTGTCTACCAAGCGAAACTAGACACAAGTAAAAGACGACTGATCATAGGCAACCGTGGAGACAATCTACCGAAGTGCTTGACTGAACAGAAGCATCAACCAATTAAACCTCGCCTGTTATCTATCTCCTCTGATGAAACGTTCTCGACAAATTTACACAACATTCAACTAGCTGATGTTGAGGTTCTAGTTTTGAATTTCAGCACAAAGAGTTATGCTTTACCCGTATTTGTGGAGAAAATGGTTAATCTGAAGGTTCTAATAGTCACAAATCATGGTTTCATACCTGCTGAATTGAGTAATTTTCACCTACTGGACTCTTTACCGAATCTGAAGAGAATCAGACTAGAGCGTGTTTCAATTCCTTCTATAACCAAGCATCCCATACAGTTGAAGAGTTTAAAGAAGATCTCTTTATTCATGTGCAGTATTGGTAAAGCTTTCTGCAATTGCTCCTTCGAAATTTCAAATGCATTGCCAAATTTAGAGGAACTGAATATTGACTATTGCAATGATTTGGTGAAATTGCCTGATGAGCTCTGCAATCTGGTTCGCCTAAAGAAGCTCAGTATCACCAACTGTCATAAGCTATCTGCCTTGCCTCAAGAGATTGGGAAACTGGAGAAGGTAGAAGTTTTGCGGCTAAGGTCTTGTACGGACTTGGTAAAACTTCCATGCTCAGTTAAAGACCTTGAAAGATTAGATTTTCTTGACATATCTGATTGCTTCAGCATTGTGGAGTTGCCTGAAGACATTGGTGAGCTGAGCAGCTTAAGAAAGATCAACATGAGACAGTGCTCTAGATTGCAAGAGCTCCCCTTATCAATTTTGGACCTTGAGAAACTAGAGGAAGTGATATGCGATGAAGAGACAGCAAGGCTGTGGGAACCCTTCCCATCCGGACTTCGCGACGGAGTGGTAAAGGAAGTCAAAGAAGAATTCAGCCTAGATTGGCTCCAAAAGATTCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

938

Amino Acids

107.29

Weight (kDa)

6.62

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 97 - 232 2.4e-29 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 295 - 451 4.4e-16 NB-ARC domain
LRR_14 PF23598 793 - 855 5.6e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 293, 1643
Acc36I ACCTGC 3 cut(s) 293, 1643, 2137
AccB7I CCANNNNNTGG 1 cut(s) 1298
AccI GTMKAC 1 cut(s) 1848
AccII CGCG 2 cut(s) 1464, 2751
AccIII TCCGGA 1 cut(s) 2741
AciI CCGC 8 cut(s) 290, 719, 796, 960, 1147, 1354, 1464, 2482
AclI AACGTT 1 cut(s) 1982
AclWI GGATC 1 cut(s) 471
AcoI YGGCCR 2 cut(s) 975, 1710
AcuI CTGAAG 7 cut(s) 419, 593, 1560, 2114, 2195, 2554, 2610
AfaI GTAC 8 cut(s) 498, 819, 843, 1074, 1099, 1107, 1182, 2497
AfiI CCNNNNNNNGG 3 cut(s) 1298, 1421, 1747
AflII CTTAAG 2 cut(s) 908, 2614
AflIII ACRYGT 3 cut(s) 114, 211, 1722
AjnI CCWGG 3 cut(s) 949, 1191, 1368
AjuI GAANNNNNNNTTGG 8 cut(s) 443, 475, 647, 679, 743, 775, 956, 988
Alw21I GWGCWC 4 cut(s) 1660, 2382, 2642, 2658
Alw26I GTCTC 7 cut(s) 653, 680, 1529, 1769, 1896, 2626, 2705
AlwI GGATC 1 cut(s) 471
AlwNI CAGNNNCTG 1 cut(s) 616
Ama87I CYCGRG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 2741
AoxI GGCC 4 cut(s) 135, 511, 975, 1710
ApaI GGGCCC 1 cut(s) 515
ApeKI GCWGC 5 cut(s) 299, 697, 957, 1144, 2610
Asp700I GAANNNNTTC 1 cut(s) 1545
AspLEI GCGC 3 cut(s) 536, 1414, 1488
AspS9I GGNCC 6 cut(s) 292, 511, 512, 790, 1189, 2674
AsuII TTCGAA 1 cut(s) 2301
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 4 cut(s) 292, 790, 1189, 2674
BaeGI GKGCMC 1 cut(s) 515
BaeI ACNNNNGTAYC 2 cut(s) 1089, 1122
BalI TGGCCA 2 cut(s) 977, 1712
BanII GRGCYC 3 cut(s) 515, 2382, 2658
BbsI GAAGAC 1 cut(s) 2598
Bbv12I GWGCWC 4 cut(s) 1660, 2382, 2642, 2658
BbvI GCAGC 5 cut(s) 286, 709, 944, 1131, 2622
BccI CCATC 4 cut(s) 433, 781, 1370, 2746
BceAI ACGGC 1 cut(s) 307
BciT130I CCWGG 3 cut(s) 951, 1193, 1370
BclI TGATCA 2 cut(s) 360, 1884
BcoDI GTCTC 7 cut(s) 653, 680, 1529, 1769, 1896, 2626, 2705
BfaI CTAG 9 cut(s) 1398, 1563, 1862, 2012, 2030, 2189, 2643, 2687, 2789
BfmI CTRYAG 2 cut(s) 300, 1798
BfoI RGCGCY 1 cut(s) 1415
BfrI CTTAAG 2 cut(s) 908, 2614
BfuAI ACCTGC 3 cut(s) 293, 1643, 2137
BglII AGATCT 2 cut(s) 1690, 2250
BisI GCNGC 6 cut(s) 300, 698, 958, 1145, 2483, 2611
BlpI GCTNAGC 1 cut(s) 2606
BlsI GCNGC 6 cut(s) 301, 699, 959, 1146, 2484, 2612
Bme1390I CCNGG 3 cut(s) 951, 1193, 1370
Bme18I GGWCC 4 cut(s) 292, 790, 1189, 2674
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 6 cut(s) 292, 511, 512, 790, 1189, 2674
BmiI GGNNCC 4 cut(s) 198, 513, 2730, 2798
BmrFI CCNGG 3 cut(s) 951, 1193, 1370
BmsI GCATC 6 cut(s) 261, 1327, 1373, 1561, 1945, 2230
BoxI GACNNNNGTC 1 cut(s) 788
BpiI GAAGAC 1 cut(s) 2598
BpmI CTGGAG 3 cut(s) 26, 972, 2483
Bpu10I CCTNAGC 2 cut(s) 516, 2486
Bpu1102I GCTNAGC 1 cut(s) 2606
Bpu14I TTCGAA 1 cut(s) 2301
BpuEI CTTGAG 2 cut(s) 2430, 2699
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 6 cut(s) 663, 759, 1368, 1369, 1618, 1897
BsaWI WCCGGW 2 cut(s) 8, 2741
Bsc4I CCNNNNNNNGG 3 cut(s) 1298, 1421, 1747
Bse1I ACTGG 4 cut(s) 43, 262, 2160, 2466
Bse3DI GCAATG 4 cut(s) 638, 1347, 2315, 2359
BseAI TCCGGA 1 cut(s) 2741
BseBI CCWGG 3 cut(s) 951, 1193, 1370
BseDI CCNNGG 6 cut(s) 663, 759, 1368, 1369, 1618, 1897
BseLI CCNNNNNNNGG 3 cut(s) 1298, 1421, 1747
BseMI GCAATG 4 cut(s) 638, 1347, 2315, 2359
BseMII CTCAG 6 cut(s) 449, 507, 1846, 2422, 2535, 2597
BseNI ACTGG 4 cut(s) 43, 262, 2160, 2466
BseRI GAGGAG 1 cut(s) 1960
BseSI GKGCMC 1 cut(s) 515
BseXI GCAGC 5 cut(s) 286, 709, 944, 1131, 2622
BseYI CCCAGC 1 cut(s) 155
BsgI GTGCAG 2 cut(s) 716, 2287
Bsh1236I CGCG 2 cut(s) 1464, 2751
Bsh1285I CGRYCG 1 cut(s) 98
BshFI GGCC 4 cut(s) 137, 513, 977, 1712
BsiEI CGRYCG 1 cut(s) 98
BsiHKAI GWGCWC 4 cut(s) 1660, 2382, 2642, 2658
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 3 cut(s) 9, 793, 2742
BslFI GGGAC 2 cut(s) 204, 796
BslI CCNNNNNNNGG 3 cut(s) 1298, 1421, 1747
BsmAI GTCTC 7 cut(s) 653, 680, 1529, 1769, 1896, 2626, 2705
BsmBI CGTCTC 1 cut(s) 680
BsmFI GGGAC 2 cut(s) 204, 796
BsnI GGCC 4 cut(s) 137, 513, 977, 1712
Bso31I GGTCTC 1 cut(s) 653
BsoBI CYCGRG 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 2301
Bsp120I GGGCCC 1 cut(s) 511
Bsp1286I GDGCHC 5 cut(s) 515, 1660, 2382, 2642, 2658
Bsp13I TCCGGA 1 cut(s) 2741
Bsp1407I TGTACA 1 cut(s) 1072
Bsp143I GATC 9 cut(s) 278, 360, 463, 1280, 1690, 1753, 1884, 2250, 2622
Bsp1720I GCTNAGC 1 cut(s) 2606
Bsp68I TCGCGA 1 cut(s) 2751
BspACI CCGC 8 cut(s) 290, 719, 796, 960, 1147, 1354, 1464, 2482
BspANI GGCC 4 cut(s) 137, 513, 977, 1712
BspCNI CTCAG 6 cut(s) 448, 508, 1845, 2421, 2534, 2598
BspEI TCCGGA 1 cut(s) 2741
BspFNI CGCG 2 cut(s) 1464, 2751
BspHI TCATGA 1 cut(s) 747
BspLI GGNNCC 4 cut(s) 198, 513, 2730, 2798
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 3 cut(s) 293, 1643, 2137
BspPI GGATC 1 cut(s) 471
BspQI GCTCTTC 1 cut(s) 387
BspT104I TTCGAA 1 cut(s) 2301
BspTI CTTAAG 2 cut(s) 908, 2614
BspTNI GGTCTC 1 cut(s) 653
BsrDI GCAATG 4 cut(s) 638, 1347, 2315, 2359
BsrGI TGTACA 1 cut(s) 1072
BsrI ACTGG 4 cut(s) 43, 262, 2160, 2466
BssECI CCNNGG 6 cut(s) 663, 759, 1368, 1369, 1618, 1897
BssMI GATC 9 cut(s) 278, 360, 463, 1280, 1690, 1753, 1884, 2250, 2622
BssT1I CCWWGG 3 cut(s) 663, 759, 1618
Bst2UI CCWGG 3 cut(s) 951, 1193, 1370
Bst6I CTCTTC 5 cut(s) 387, 612, 2171, 2231, 2703
BstAFI CTTAAG 2 cut(s) 908, 2614
BstAPI GCANNNNNTGC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 1072
BstBI TTCGAA 1 cut(s) 2301
BstC8I GCNNGC 3 cut(s) 26, 153, 1842
BstDSI CCRYGG 1 cut(s) 1897
BstEII GGTNACC 1 cut(s) 754
BstFNI CGCG 2 cut(s) 1464, 2751
BstH2I RGCGCY 1 cut(s) 1415
BstHHI GCGC 3 cut(s) 536, 1414, 1488
BstKTI GATC 9 cut(s) 281, 363, 466, 1283, 1693, 1756, 1887, 2253, 2625
BstMAI GTCTC 7 cut(s) 653, 680, 1529, 1769, 1896, 2626, 2705
BstMBI GATC 9 cut(s) 278, 360, 463, 1280, 1690, 1753, 1884, 2250, 2622
BstMCI CGRYCG 1 cut(s) 98
BstMWI GCNNNNNNNGC 4 cut(s) 296, 308, 1837, 2377
BstNI CCWGG 3 cut(s) 951, 1193, 1370
BstNSI RCATGY 3 cut(s) 118, 215, 1726
BstPAI GACNNNNGTC 1 cut(s) 788
BstPI GGTNACC 1 cut(s) 754
BstSCI CCNGG 3 cut(s) 949, 1191, 1368
BstSFI CTRYAG 2 cut(s) 300, 1798
BstSLI GKGCMC 1 cut(s) 515
BstUI CGCG 2 cut(s) 1464, 2751
BstV1I GCAGC 5 cut(s) 286, 709, 944, 1131, 2622
BstV2I GAAGAC 1 cut(s) 2598
BstX2I RGATCY 2 cut(s) 1690, 2250
BstYI RGATCY 2 cut(s) 1690, 2250
BsuRI GGCC 4 cut(s) 137, 513, 977, 1712
BtgI CCRYGG 1 cut(s) 1897
BtgZI GCGATG 1 cut(s) 2718
BtsIMutI CAGTG 6 cut(s) 255, 425, 1038, 1785, 1807, 2642
BtuMI TCGCGA 1 cut(s) 2751
BveI ACCTGC 3 cut(s) 293, 1643, 2137
Cac8I GCNNGC 3 cut(s) 26, 153, 1842
CaiI CAGNNNCTG 1 cut(s) 616
CciI TCATGA 1 cut(s) 747
CfoI GCGC 3 cut(s) 536, 1414, 1488
Cfr13I GGNCC 6 cut(s) 292, 511, 512, 790, 1189, 2674
Csp6I GTAC 8 cut(s) 497, 818, 842, 1073, 1098, 1106, 1181, 2496
CspCI CAANNNNNGTGG 4 cut(s) 13, 48, 1425, 1460
CviAII CATG 9 cut(s) 115, 212, 748, 1723, 1823, 2117, 2263, 2517, 2629
CviQI GTAC 8 cut(s) 497, 818, 842, 1073, 1098, 1106, 1181, 2496
DpnI GATC 9 cut(s) 280, 362, 465, 1282, 1692, 1755, 1886, 2252, 2624
DpnII GATC 9 cut(s) 278, 360, 463, 1280, 1690, 1753, 1884, 2250, 2622
DraI TTTAAA 2 cut(s) 493, 2244
EaeI YGGCCR 2 cut(s) 975, 1710
Eam1104I CTCTTC 5 cut(s) 387, 612, 2171, 2231, 2703
EarI CTCTTC 5 cut(s) 387, 612, 2171, 2231, 2703
EciI GGCGGA 3 cut(s) 305, 811, 1343
Ecl136II GAGCTC 2 cut(s) 2380, 2656
Eco130I CCWWGG 3 cut(s) 663, 759, 1618
Eco24I GRGCYC 3 cut(s) 515, 2382, 2658
Eco31I GGTCTC 1 cut(s) 653
Eco32I GATATC 1 cut(s) 1427
Eco47I GGWCC 4 cut(s) 292, 790, 1189, 2674
Eco53kI GAGCTC 2 cut(s) 2380, 2656
Eco57I CTGAAG 7 cut(s) 419, 593, 1560, 2114, 2195, 2554, 2610
Eco88I CYCGRG 1 cut(s) 80
Eco91I GGTNACC 1 cut(s) 754
EcoICRI GAGCTC 2 cut(s) 2380, 2656
EcoO109I RGGNCCY 2 cut(s) 511, 512
EcoO65I GGTNACC 1 cut(s) 754
EcoRI GAATTC 3 cut(s) 722, 1644, 2780
EcoRII CCWGG 3 cut(s) 949, 1191, 1368
EcoRV GATATC 1 cut(s) 1427
EcoT14I CCWWGG 3 cut(s) 663, 759, 1618
EcoT22I ATGCAT 1 cut(s) 2317
EcoT38I GRGCYC 3 cut(s) 515, 2382, 2658
ErhI CCWWGG 3 cut(s) 663, 759, 1618
Esp3I CGTCTC 1 cut(s) 680
FaeI CATG 9 cut(s) 118, 215, 751, 1726, 1826, 2120, 2266, 2520, 2632
FalI AAGNNNNNCTT 8 cut(s) 647, 679, 743, 775, 1043, 1075, 1533, 1565
FaqI GGGAC 2 cut(s) 204, 796
FatI CATG 9 cut(s) 114, 211, 747, 1722, 1822, 2116, 2262, 2516, 2628
FauI CCCGC 1 cut(s) 953
FbaI TGATCA 2 cut(s) 360, 1884
FblI GTMKAC 1 cut(s) 1848
Fnu4HI GCNGC 6 cut(s) 300, 698, 958, 1145, 2483, 2611
FriOI GRGCYC 3 cut(s) 515, 2382, 2658
Fsp4HI GCNGC 6 cut(s) 300, 698, 958, 1145, 2483, 2611
FspBI CTAG 9 cut(s) 1398, 1563, 1862, 2012, 2030, 2189, 2643, 2687, 2789
GlaI GCGC 3 cut(s) 535, 1413, 1487
GluI GCNGC 6 cut(s) 300, 698, 958, 1145, 2483, 2611
GsaI CCCAGC 1 cut(s) 159
GsuI CTGGAG 3 cut(s) 26, 972, 2483
HaeII RGCGCY 1 cut(s) 1415
HaeIII GGCC 4 cut(s) 137, 513, 977, 1712
HapII CCGG 3 cut(s) 9, 793, 2742
HhaI GCGC 3 cut(s) 536, 1414, 1488
Hin1II CATG 9 cut(s) 118, 215, 751, 1726, 1826, 2120, 2266, 2520, 2632
Hin6I GCGC 3 cut(s) 534, 1412, 1486
HinP1I GCGC 3 cut(s) 534, 1412, 1486
HincII GTYRAC 1 cut(s) 1056
HindII GTYRAC 1 cut(s) 1056
HindIII AAGCTT 4 cut(s) 231, 414, 902, 2280
HpaII CCGG 3 cut(s) 9, 793, 2742
Hpy166II GTNNAC 4 cut(s) 327, 1056, 1805, 1849
Hpy8I GTNNAC 4 cut(s) 327, 1056, 1805, 1849
Hpy99I CGWCG 2 cut(s) 1106, 2756
HpyCH4IV ACGT 2 cut(s) 1104, 1982
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 308, 1837, 2377
HpySE526I ACGT 2 cut(s) 1104, 1982
Hsp92II CATG 9 cut(s) 118, 215, 751, 1726, 1826, 2120, 2266, 2520, 2632
HspAI GCGC 3 cut(s) 534, 1412, 1486
Kpn2I TCCGGA 1 cut(s) 2741
Ksp22I TGATCA 2 cut(s) 360, 1884
Kzo9I GATC 9 cut(s) 278, 360, 463, 1280, 1690, 1753, 1884, 2250, 2622
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 7 cut(s) 105, 148, 196, 952, 2300, 2661, 2802
Lsp1109I GCAGC 5 cut(s) 286, 709, 944, 1131, 2622
LweI GCATC 6 cut(s) 261, 1327, 1373, 1561, 1945, 2230
MaeI CTAG 9 cut(s) 1398, 1563, 1862, 2012, 2030, 2189, 2643, 2687, 2789
MaeII ACGT 2 cut(s) 1104, 1982
MaeIII GTNAC 8 cut(s) 754, 859, 1042, 1126, 1270, 1762, 1813, 2107
MalI GATC 9 cut(s) 280, 362, 465, 1282, 1692, 1755, 1886, 2252, 2624
MboI GATC 9 cut(s) 278, 360, 463, 1280, 1690, 1753, 1884, 2250, 2622
MfeI CAATTG 2 cut(s) 183, 2290
MflI RGATCY 2 cut(s) 1690, 2250
MhlI GDGCHC 5 cut(s) 515, 1660, 2382, 2642, 2658
MlsI TGGCCA 2 cut(s) 977, 1712
MluNI TGGCCA 2 cut(s) 977, 1712
MlyI GAGTC 2 cut(s) 1339, 2153
MmeI TCCRAC 5 cut(s) 165, 441, 649, 847, 1429
Mox20I TGGCCA 2 cut(s) 977, 1712
Mph1103I ATGCAT 1 cut(s) 2317
MroI TCCGGA 1 cut(s) 2741
MroXI GAANNNNTTC 1 cut(s) 1545
MscI TGGCCA 2 cut(s) 977, 1712
MslI CAYNNNNRTG 2 cut(s) 752, 1501
Msp20I TGGCCA 2 cut(s) 977, 1712
MspA1I CMGCKG 1 cut(s) 447
MspCI CTTAAG 2 cut(s) 908, 2614
MspI CCGG 3 cut(s) 9, 793, 2742
MspR9I CCNGG 3 cut(s) 951, 1193, 1370
MunI CAATTG 2 cut(s) 183, 2290
MvaI CCWGG 3 cut(s) 951, 1193, 1370
MvnI CGCG 2 cut(s) 1464, 2751
MwoI GCNNNNNNNGC 4 cut(s) 296, 308, 1837, 2377
NdeII GATC 9 cut(s) 278, 360, 463, 1280, 1690, 1753, 1884, 2250, 2622
NlaIII CATG 9 cut(s) 118, 215, 751, 1726, 1826, 2120, 2266, 2520, 2632
NlaIV GGNNCC 4 cut(s) 198, 513, 2730, 2798
NmuCI GTSAC 5 cut(s) 754, 1270, 1762, 1813, 2107
NruI TCGCGA 1 cut(s) 2751
NsiI ATGCAT 1 cut(s) 2317
NspI RCATGY 3 cut(s) 118, 215, 1726
NspV TTCGAA 1 cut(s) 2301
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 1 cut(s) 747
PaqCI CACCTGC 2 cut(s) 293, 1643
PasI CCCWGGG 1 cut(s) 1369
PciI ACATGT 3 cut(s) 114, 211, 1722
PciSI GCTCTTC 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 1545
PflMI CCANNNNNTGG 1 cut(s) 1298
PfoI TCCNGGA 2 cut(s) 949, 1191
PkrI GCNGC 6 cut(s) 301, 699, 959, 1146, 2484, 2612
PleI GAGTC 2 cut(s) 1339, 2153
PpsI GAGTC 2 cut(s) 1339, 2153
PscI ACATGT 3 cut(s) 114, 211, 1722
PshAI GACNNNNGTC 1 cut(s) 788
Psp124BI GAGCTC 2 cut(s) 2382, 2658
Psp1406I AACGTT 1 cut(s) 1982
Psp6I CCWGG 3 cut(s) 949, 1191, 1368
PspEI GGTNACC 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 3 cut(s) 949, 1191, 1368
PspN4I GGNNCC 4 cut(s) 198, 513, 2730, 2798
PspOMI GGGCCC 1 cut(s) 511
PspPI GGNCC 6 cut(s) 292, 511, 512, 790, 1189, 2674
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 616
PsuI RGATCY 2 cut(s) 1690, 2250
PvuII CAGCTG 1 cut(s) 447
RruI TCGCGA 1 cut(s) 2751
RsaI GTAC 8 cut(s) 498, 819, 843, 1074, 1099, 1107, 1182, 2497
RsaNI GTAC 8 cut(s) 497, 818, 842, 1073, 1098, 1106, 1181, 2496
RseI CAYNNNNRTG 2 cut(s) 752, 1501
SacI GAGCTC 2 cut(s) 2382, 2658
SapI GCTCTTC 1 cut(s) 387
SatI GCNGC 6 cut(s) 300, 698, 958, 1145, 2483, 2611
Sau3AI GATC 9 cut(s) 278, 360, 463, 1280, 1690, 1753, 1884, 2250, 2622
Sau96I GGNCC 6 cut(s) 292, 511, 512, 790, 1189, 2674
SchI GAGTC 2 cut(s) 1339, 2153
ScrFI CCNGG 3 cut(s) 951, 1193, 1370
SduI GDGCHC 5 cut(s) 515, 1660, 2382, 2642, 2658
SfaNI GCATC 6 cut(s) 261, 1327, 1373, 1561, 1945, 2230
SfcI CTRYAG 2 cut(s) 300, 1798
Sfr274I CTCGAG 1 cut(s) 80
SfuI TTCGAA 1 cut(s) 2301
SinI GGWCC 4 cut(s) 292, 790, 1189, 2674
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 2 cut(s) 752, 1501
SmlI CTYRAG 5 cut(s) 80, 908, 2445, 2614, 2678
SmoI CTYRAG 5 cut(s) 80, 908, 2445, 2614, 2678
SsiI CCGC 8 cut(s) 290, 719, 796, 960, 1147, 1354, 1464, 2482
SspI AATATT 2 cut(s) 2341, 2813
SspMI CTAG 9 cut(s) 1398, 1563, 1862, 2012, 2030, 2189, 2643, 2687, 2789
SstI GAGCTC 2 cut(s) 2382, 2658
StyD4I CCNGG 3 cut(s) 949, 1191, 1368
StyI CCWWGG 3 cut(s) 663, 759, 1618
TaiI ACGT 2 cut(s) 1107, 1985
TaqI TCGA 5 cut(s) 81, 371, 1695, 1988, 2301
TaqII GACCGA 1 cut(s) 84
TatI WGTACW 3 cut(s) 496, 1072, 1180
TauI GCSGC 1 cut(s) 2485
TscAI CASTG 6 cut(s) 262, 432, 1045, 1785, 1807, 2642
TseFI GTSAC 5 cut(s) 754, 1270, 1762, 1813, 2107
TseI GCWGC 5 cut(s) 299, 697, 957, 1144, 2610
Tsp45I GTSAC 5 cut(s) 754, 1270, 1762, 1813, 2107
TspGWI ACGGA 4 cut(s) 92, 206, 2513, 2769
TspRI CASTG 6 cut(s) 262, 432, 1045, 1785, 1807, 2642
Van91I CCANNNNNTGG 1 cut(s) 1298
Vha464I CTTAAG 2 cut(s) 908, 2614
VpaK11BI GGWCC 4 cut(s) 292, 790, 1189, 2674
XbaI TCTAGA 1 cut(s) 2642
XceI RCATGY 3 cut(s) 118, 215, 1726
XcmI CCANNNNNNNNNTGG 1 cut(s) 1744
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 1848
XmnI GAANNNNTTC 1 cut(s) 1545
XspI CTAG 9 cut(s) 1398, 1563, 1862, 2012, 2030, 2189, 2643, 2687, 2789
Zsp2I ATGCAT 1 cut(s) 2317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.