RchiOBHm_Chr3g0485281

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000039
Physical Location & Seq
Reverse (-)
31935776 .. 31940830
5055 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44990

Sequence Viewer

Length: 2421 bp
ATGGCAGAGGCCGCGGAGCCGGTAATGGGGACTGCTATATCGTATAATGCGCTGTATGAGGAAGTTAATGATATGATGGCCAAGAATTTAATTTTCCAGCCCCTCCTCAAAGATCTGAAATCCAAGCTAGACACCTTACAAGTATTCTTCCAAGACATGGCGCGATATAAGCATTCACACCCACAAAAGGAGGAACTAGAAGCATGTAGAACACAAATAGAACAGGGCAGAACGGTGATTCACAAGTGCTCCCACGTTAGCAAATGGAATATGTATAAAAAGAACAAGTATACTAACCAACTACTAGGATTGATAAAATCTCTTGAAAGATTGTCAGAAATACTGAAAGGTACTCAGGTGGAAATGGATGTGAAGGAGTCCTTGGTTGCGATAAGGAATATAGAAACCATGTTTGAGACAAAAGCTTTGTCCACAATACCTGAACTCCCATCCTTTGTTGTTGGATTTGATGTGCATTTGAGCGAACTGAAGATCAAGCTTCTCAGTAACGATGAAGCATCAATGCTAGTGCTGACTGGTATGGGAGGATGCGGGAAAACCCTATTGGCAACACTATTTTGTCAGGATGACGCAGTCAAAGATAAATTCAAGGACAACATCTTCTTTGTCACCGTATCAAGAAATCTCAACTTTGAACATATTGTTCAGCAACTATATCAACAGAAGCGTTCTCCGGTACCTGCTTTGGAACAGAAGCGTTCTCCGGTACCTGCTTTGGAACAGAAGCGTTCTCCGGTACCTACATTCCAAAGCAAGACAATGGCAGTTACTTGTCTACAACAGTTTTTAAAAGCGGAAGGAAATAAACCTTTATTGTTGGTATTGGATGATGTTTGGTCTGGATCCGAGTCCCTTCTTGAAGTAGTTGATGAATTGAAAATGCCAAATTACAAAATTTTGATTACATCAAGATCTTCATTTCCAAGATTTTCCTCTCCATACTACATGGAACCACTGAATTATGAGAATGCAATGACCCTGTTTCATCATTCAGCATCCTTGGGAGATAAGAGCTCTTATATTCCACACACTCTTTCAAGAAAGGTACTGGAGCAGTGTAAGGGGATTCCACTTGTCATCACAGCAATTGGAAGATCACTTTGTGCTCAACCTGTAGAGATGTGGAAAAGAAGAGTATCTGAATTGTCTAAAAATCACTCTGTTCTTGAATATGAGACTGAAGTGTTTCTTTGCCTCAAAAGTAGCCTAGATGCTTTGGACGAAATGCCTATCCTAAAAAAATGTTTCTTTGATCTTGCTTCATTTCCTAGCAACCGAAGAATCCCTGTTGCTGCTCTCATTGATATGTGGATAGAGTTATACGATCTAGAGGAAGAAATTTTGTGCATTGGGAACATTTATGATCTCACCACCCGAAGTTTAGCTAATGTTGTAGTCCCAAGGAAGCTGGAAGAAAAGGAAGGAGATGGATACTACATTGACTATTTTGTTACCCAGCATGATTTTCTTAGAGATCTAGCTATTTGTCATTGTAAACGAGAGAGCATACAGCACGGAGAAAAGCTGTTTATTGACTTACACGGAAACAATTTACCCAAGTGGTGGAGGGAACAGAAGTATCAGTCCATTGAAGCTCGCCTAGCGTCTATCTCAACAGATGAAAGCTTCTCAATGGCATGGCCCTACATGCACCTACCCGAAGCCGAGGTTCTAATCTTGAATTTTGATACAAACAACTATGCCTTGCCTGAGTTTGTGGAGGGCATGGTTGAGCTGAAGGTACTTGTGGTGACAAACTATGGTTCTTTACCTGTTGAATTAAGAAATTTTCAACTGTTGAGTTCGTTACCAAAACTGAAGAGAATCAGACTAGAGCGCATTTCAATTGCTTCCATAACCAACATCCCAATCGAATTGAAGAGAGTACAGAAGATATCATTGTTCATGTGCGGCATCGGTCAAGCTTTCAGCAATTGTCCTATCAAAATTTCAGATGCATTTCCAAATCTAGAAGAAATGAACATTGACTATTGCAATGATTTGGTGGAATTGCCTACAGAGCTTTGTGATCTTGTTCACCTTAAGAGACTCTGCATCACCAACTGTCATAAGCTATCTGCATTGCCTGTAGAGATAGGGAAGTTGATCAATTTGGAAGTTTTGAGACTAAGGTCTTGTAAAAACTTGGAAGGTTTACCATGCTCGATAAAGAACCTGGGAAAGTTAAACTTCTTGGATATAGCGGAATGCTCTAGGTTGCAAGAGCTGCCTGAATCAGTTTTGGCTCTTGAGCAACTAAAGGAAGTGATATGTGATGAAGATATGGAAGGACAATGGACAATCACTCAGCCCAACATAAGGATAAAGGTCCTCAAAGAAGAAGTCAACCAAAATTGGCTCTCACTCATGAAAGATGCCTTAAATGTTGGGCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

806

Amino Acids

92.17

Weight (kDa)

5.84

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 16 - 136 6.1e-13 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 159 - 313 3e-14 NB-ARC domain
NPHP3_N PF24883 169 - 311 3e-06 Nephrocystin 3, N-terminal
AAA_16 PF13191 173 - 285 2e-06 AAA ATPase domain
LRR_14 PF23598 659 - 766 3.4e-12 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 709, 739
Acc65I GGTACC 3 cut(s) 697, 727, 757
AccB1I GGYRCC 3 cut(s) 697, 727, 757
AccB7I CCANNNNNTGG 2 cut(s) 157, 1584
AccI GTMKAC 2 cut(s) 290, 796
AccII CGCG 2 cut(s) 14, 163
AciI CCGC 6 cut(s) 12, 14, 552, 815, 1932, 2225
AclWI GGATC 2 cut(s) 858, 871
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 7 cut(s) 85, 605, 915, 1359, 1702, 1807, 1968
AcuI CTGAAG 4 cut(s) 509, 1221, 1778, 1859
AdeI CACNNNGTG 1 cut(s) 1124
AfaI GTAC 7 cut(s) 352, 699, 729, 759, 1068, 1764, 1908
AfiI CCNNNNNNNGG 5 cut(s) 26, 157, 187, 1584, 2340
AflII CTTAAG 1 cut(s) 2063
AjnI CCWGG 1 cut(s) 2196
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 548, 580
Alw21I GWGCWC 3 cut(s) 251, 1037, 1129
Alw26I GTCTC 4 cut(s) 410, 1190, 2062, 2140
AlwI GGATC 2 cut(s) 858, 871
AoxI GGCC 3 cut(s) 9, 78, 1661
ApeKI GCWGC 2 cut(s) 1313, 2248
ApoI RAATTY 7 cut(s) 85, 605, 915, 1359, 1702, 1807, 1968
ArsI GACNNNNNNTTYG 6 cut(s) 409, 413, 441, 445, 2349, 2381
Asp700I GAANNNNTTC 1 cut(s) 1206
Asp718I GGTACC 3 cut(s) 697, 727, 757
AspLEI GCGC 3 cut(s) 52, 163, 1860
AspS9I GGNCC 2 cut(s) 1662, 2350
AsuHPI GGTGA 6 cut(s) 247, 622, 1381, 1783, 2051, 2071
AvaII GGWCC 1 cut(s) 2350
BaeI ACNNNNGTAYC 4 cut(s) 342, 375, 1583, 1616
BalI TGGCCA 1 cut(s) 80
BamHI GGATCC 1 cut(s) 863
BanI GGYRCC 3 cut(s) 697, 727, 757
BanII GRGCYC 1 cut(s) 1037
Bbv12I GWGCWC 3 cut(s) 251, 1037, 1129
BbvI GCAGC 2 cut(s) 1300, 2235
BccI CCATC 3 cut(s) 70, 457, 1442
BciT130I CCWGG 1 cut(s) 2198
BciVI GTATCC 1 cut(s) 1445
BclI TGATCA 1 cut(s) 2127
BcoDI GTCTC 4 cut(s) 410, 1190, 2062, 2140
BfmI CTRYAG 3 cut(s) 1134, 2037, 2109
BfrI CTTAAG 1 cut(s) 2063
BfuAI ACCTGC 2 cut(s) 709, 739
BfuI GTATCC 1 cut(s) 1445
BglII AGATCT 3 cut(s) 112, 932, 1495
BisI GCNGC 4 cut(s) 12, 1314, 1933, 2249
BlsI GCNGC 4 cut(s) 13, 1315, 1934, 2250
Bme1390I CCNGG 1 cut(s) 2198
Bme18I GGWCC 1 cut(s) 2350
BmgT120I GGNCC 2 cut(s) 1662, 2350
BmiI GGNNCC 6 cut(s) 18, 699, 729, 759, 865, 972
BmrFI CCNGG 1 cut(s) 2198
BmsI GCATC 8 cut(s) 527, 539, 1025, 1222, 1944, 1966, 2085, 2386
BoxI GACNNNNGTC 1 cut(s) 2152
BpmI CTGGAG 1 cut(s) 1091
BpuEI CTTGAG 1 cut(s) 2291
BsaJI CCNNGG 6 cut(s) 12, 381, 1020, 1421, 1686, 2197
BsaWI WCCGGW 3 cut(s) 694, 724, 754
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 429, 459
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 157, 187, 1584, 2340
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 541, 1074
Bse3DI GCAATG 4 cut(s) 999, 2023, 2102, 2420
BseBI CCWGG 1 cut(s) 2198
BseDI CCNNGG 6 cut(s) 12, 381, 1020, 1421, 1686, 2197
BseGI GGATG 7 cut(s) 373, 449, 554, 592, 853, 1016, 1884
BseLI CCNNNNNNNGG 5 cut(s) 26, 157, 187, 1584, 2340
BseMI GCAATG 4 cut(s) 999, 2023, 2102, 2420
BseMII CTCAG 4 cut(s) 368, 517, 1722, 2342
BseNI ACTGG 2 cut(s) 541, 1074
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 2 cut(s) 1300, 2235
BseYI CCCAGC 1 cut(s) 1476
Bsh1236I CGCG 2 cut(s) 14, 163
BshFI GGCC 3 cut(s) 11, 80, 1663
BshNI GGYRCC 3 cut(s) 697, 727, 757
BsiHKAI GWGCWC 3 cut(s) 251, 1037, 1129
BsiSI CCGG 4 cut(s) 20, 695, 725, 755
BslFI GGGAC 3 cut(s) 43, 856, 1403
BslI CCNNNNNNNGG 5 cut(s) 26, 157, 187, 1584, 2340
BsmAI GTCTC 4 cut(s) 410, 1190, 2062, 2140
BsmFI GGGAC 3 cut(s) 43, 856, 1403
BsmI GAATGC 3 cut(s) 172, 994, 2234
BsnI GGCC 3 cut(s) 11, 80, 1663
Bsp1286I GDGCHC 3 cut(s) 251, 1037, 1129
BspACI CCGC 6 cut(s) 12, 14, 552, 815, 1932, 2225
BspANI GGCC 3 cut(s) 11, 80, 1663
BspCNI CTCAG 4 cut(s) 367, 516, 1723, 2341
BspFNI CGCG 2 cut(s) 14, 163
BspHI TCATGA 1 cut(s) 2388
BspLI GGNNCC 6 cut(s) 18, 699, 729, 759, 865, 972
BspMI ACCTGC 2 cut(s) 709, 739
BspPI GGATC 2 cut(s) 858, 871
BspT107I GGYRCC 3 cut(s) 697, 727, 757
BspTI CTTAAG 1 cut(s) 2063
BsrDI GCAATG 4 cut(s) 999, 2023, 2102, 2420
BsrFI RCCGGY 1 cut(s) 19
BsrI ACTGG 2 cut(s) 541, 1074
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 6 cut(s) 12, 381, 1020, 1421, 1686, 2197
BssNAI GTATAC 1 cut(s) 291
BssT1I CCWWGG 3 cut(s) 381, 1020, 1421
Bst1107I GTATAC 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 2198
Bst4CI ACNGT 5 cut(s) 235, 634, 804, 1818, 2087
Bst6I CTCTTC 3 cut(s) 1147, 1835, 1895
BstAFI CTTAAG 1 cut(s) 2063
BstAPI GCANNNNNTGC 1 cut(s) 2248
BstC8I GCNNGC 1 cut(s) 1618
BstDEI CTNAG 6 cut(s) 354, 503, 1490, 1731, 2150, 2328
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 7 cut(s) 373, 449, 554, 592, 853, 1016, 1884
BstFNI CGCG 2 cut(s) 14, 163
BstHHI GCGC 3 cut(s) 52, 163, 1860
BstMAI GTCTC 4 cut(s) 410, 1190, 2062, 2140
BstMWI GCNNNNNNNGC 6 cut(s) 11, 169, 1622, 1669, 2041, 2248
BstNI CCWGG 1 cut(s) 2198
BstNSI RCATGY 2 cut(s) 207, 1672
BstPAI GACNNNNGTC 1 cut(s) 2152
BstSCI CCNGG 1 cut(s) 2196
BstSFI CTRYAG 3 cut(s) 1134, 2037, 2109
BstUI CGCG 2 cut(s) 14, 163
BstV1I GCAGC 2 cut(s) 1300, 2235
BstX2I RGATCY 4 cut(s) 112, 863, 932, 1495
BstYI RGATCY 4 cut(s) 112, 863, 932, 1495
BstZ17I GTATAC 1 cut(s) 291
BsuI GTATCC 1 cut(s) 1445
BsuRI GGCC 3 cut(s) 11, 80, 1663
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 7 cut(s) 373, 449, 554, 592, 853, 1016, 1884
BtsI GCAGTG 1 cut(s) 1082
BtsIMutI CAGTG 2 cut(s) 974, 1082
BveI ACCTGC 2 cut(s) 709, 739
Cac8I GCNNGC 1 cut(s) 1618
CciI TCATGA 1 cut(s) 2388
CfoI GCGC 3 cut(s) 52, 163, 1860
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 1662, 2350
Cfr42I CCGCGG 1 cut(s) 15
CseI GACGC 2 cut(s) 599, 1614
Csp6I GTAC 7 cut(s) 351, 698, 728, 758, 1067, 1763, 1907
CviQI GTAC 7 cut(s) 351, 698, 728, 758, 1067, 1763, 1907
DdeI CTNAG 6 cut(s) 354, 503, 1490, 1731, 2150, 2328
DraI TTTAAA 1 cut(s) 810
DraIII CACNNNGTG 1 cut(s) 1124
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 3 cut(s) 1147, 1835, 1895
EarI CTCTTC 3 cut(s) 1147, 1835, 1895
Ecl136II GAGCTC 1 cut(s) 1035
Eco130I CCWWGG 3 cut(s) 381, 1020, 1421
Eco24I GRGCYC 1 cut(s) 1037
Eco32I GATATC 1 cut(s) 1917
Eco47I GGWCC 1 cut(s) 2350
Eco53kI GAGCTC 1 cut(s) 1035
Eco57I CTGAAG 4 cut(s) 509, 1221, 1778, 1859
EcoICRI GAGCTC 1 cut(s) 1035
EcoO109I RGGNCCY 1 cut(s) 2350
EcoRII CCWGG 1 cut(s) 2196
EcoRV GATATC 1 cut(s) 1917
EcoT14I CCWWGG 3 cut(s) 381, 1020, 1421
EcoT22I ATGCAT 1 cut(s) 1981
EcoT38I GRGCYC 1 cut(s) 1037
ErhI CCWWGG 3 cut(s) 381, 1020, 1421
FalI AAGNNNNNCTT 6 cut(s) 365, 397, 1194, 1226, 2195, 2227
FaqI GGGAC 3 cut(s) 43, 856, 1403
FauI CCCGC 1 cut(s) 545
FbaI TGATCA 1 cut(s) 2127
FblI GTMKAC 2 cut(s) 290, 796
Fnu4HI GCNGC 4 cut(s) 12, 1314, 1933, 2249
FokI GGATG 7 cut(s) 380, 436, 561, 599, 860, 1003, 1871
FriOI GRGCYC 1 cut(s) 1037
Fsp4HI GCNGC 4 cut(s) 12, 1314, 1933, 2249
GlaI GCGC 3 cut(s) 51, 162, 1859
GluI GCNGC 4 cut(s) 12, 1314, 1933, 2249
GsaI CCCAGC 1 cut(s) 1480
GsuI CTGGAG 1 cut(s) 1091
HaeIII GGCC 3 cut(s) 11, 80, 1663
HapII CCGG 4 cut(s) 20, 695, 725, 755
HgaI GACGC 2 cut(s) 599, 1614
HhaI GCGC 3 cut(s) 52, 163, 1860
Hin6I GCGC 3 cut(s) 50, 161, 1858
HinP1I GCGC 3 cut(s) 50, 161, 1858
HincII GTYRAC 1 cut(s) 2368
HindII GTYRAC 1 cut(s) 2368
HindIII AAGCTT 4 cut(s) 423, 497, 1645, 1944
HinfI GANTC 8 cut(s) 238, 377, 869, 1087, 1302, 1845, 2070, 2255
HpaII CCGG 4 cut(s) 20, 695, 725, 755
HphI GGTGA 6 cut(s) 247, 622, 1381, 1783, 2051, 2071
Hpy166II GTNNAC 7 cut(s) 291, 432, 797, 1517, 2059, 2177, 2368
Hpy188I TCNGA 6 cut(s) 117, 337, 868, 1162, 1850, 1975
Hpy8I GTNNAC 7 cut(s) 291, 432, 797, 1517, 2059, 2177, 2368
HpyAV CCTTC 7 cut(s) 367, 812, 884, 1436, 1753, 2165, 2303
HpyCH4III ACNGT 5 cut(s) 235, 634, 804, 1818, 2087
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 9 cut(s) 475, 992, 1368, 1672, 1979, 2016, 2076, 2102, 2242
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 169, 1622, 1669, 2041, 2248
HpyF3I CTNAG 6 cut(s) 354, 503, 1490, 1731, 2150, 2328
HpySE526I ACGT 1 cut(s) 255
HspAI GCGC 3 cut(s) 50, 161, 1858
KpnI GGTACC 3 cut(s) 701, 731, 761
Ksp22I TGATCA 1 cut(s) 2127
KspI CCGCGG 1 cut(s) 15
LmnI GCTCC 3 cut(s) 16, 254, 1072
Lsp1109I GCAGC 2 cut(s) 1300, 2235
LweI GCATC 8 cut(s) 527, 539, 1025, 1222, 1944, 1966, 2085, 2386
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 6 cut(s) 506, 628, 787, 1471, 1771, 1827
MfeI CAATTG 3 cut(s) 1107, 1866, 1954
MflI RGATCY 4 cut(s) 112, 863, 932, 1495
MhlI GDGCHC 3 cut(s) 251, 1037, 1129
MlsI TGGCCA 1 cut(s) 80
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 3 cut(s) 386, 878, 2064
MmeI TCCRAC 1 cut(s) 442
Mox20I TGGCCA 1 cut(s) 80
Mph1103I ATGCAT 1 cut(s) 1981
MroXI GAANNNNTTC 1 cut(s) 1206
MscI TGGCCA 1 cut(s) 80
MseI TTAA 8 cut(s) 66, 89, 809, 1802, 2064, 2207, 2402, 2419
MslI CAYNNNNRTG 1 cut(s) 1325
Msp20I TGGCCA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 2063
MspI CCGG 4 cut(s) 20, 695, 725, 755
MspR9I CCNGG 1 cut(s) 2198
MunI CAATTG 3 cut(s) 1107, 1866, 1954
Mva1269I GAATGC 3 cut(s) 172, 994, 2234
MvaI CCWGG 1 cut(s) 2198
MvnI CGCG 2 cut(s) 14, 163
MwoI GCNNNNNNNGC 6 cut(s) 11, 169, 1622, 1669, 2041, 2248
NlaIV GGNNCC 6 cut(s) 18, 699, 729, 759, 865, 972
NmeAIII GCCGAG 1 cut(s) 1711
NmuCI GTSAC 2 cut(s) 628, 1771
NsiI ATGCAT 1 cut(s) 1981
NspI RCATGY 2 cut(s) 207, 1672
PagI TCATGA 1 cut(s) 2388
PctI GAATGC 3 cut(s) 172, 994, 2234
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 5 cut(s) 238, 1087, 1302, 1845, 2255
PflFI GACNNNGTC 1 cut(s) 593
PflMI CCANNNNNTGG 2 cut(s) 157, 1584
PkrI GCNGC 4 cut(s) 13, 1315, 1934, 2250
PleI GAGTC 3 cut(s) 385, 877, 2064
PpsI GAGTC 3 cut(s) 385, 877, 2064
PpuMI RGGWCCY 1 cut(s) 2350
PshAI GACNNNNGTC 1 cut(s) 2152
Psp124BI GAGCTC 1 cut(s) 1037
Psp5II RGGWCCY 1 cut(s) 2350
Psp6I CCWGG 1 cut(s) 2196
PspFI CCCAGC 1 cut(s) 1476
PspGI CCWGG 1 cut(s) 2196
PspN4I GGNNCC 6 cut(s) 18, 699, 729, 759, 865, 972
PspPI GGNCC 2 cut(s) 1662, 2350
PspPPI RGGWCCY 1 cut(s) 2350
PsuI RGATCY 4 cut(s) 112, 863, 932, 1495
PsyI GACNNNGTC 1 cut(s) 593
RsaI GTAC 7 cut(s) 352, 699, 729, 759, 1068, 1764, 1908
RsaNI GTAC 7 cut(s) 351, 698, 728, 758, 1067, 1763, 1907
RseI CAYNNNNRTG 1 cut(s) 1325
SacI GAGCTC 1 cut(s) 1037
SacII CCGCGG 1 cut(s) 15
SaqAI TTAA 8 cut(s) 66, 89, 809, 1802, 2064, 2207, 2402, 2419
SatI GCNGC 4 cut(s) 12, 1314, 1933, 2249
Sau96I GGNCC 2 cut(s) 1662, 2350
SchI GAGTC 3 cut(s) 386, 878, 2064
ScrFI CCNGG 1 cut(s) 2198
SduI GDGCHC 3 cut(s) 251, 1037, 1129
SfaNI GCATC 8 cut(s) 527, 539, 1025, 1222, 1944, 1966, 2085, 2386
SfcI CTRYAG 3 cut(s) 1134, 2037, 2109
Sfr303I CCGCGG 1 cut(s) 15
SgrBI CCGCGG 1 cut(s) 15
SinI GGWCC 1 cut(s) 2350
SmiMI CAYNNNNRTG 1 cut(s) 1325
SmlI CTYRAG 2 cut(s) 2063, 2270
SmoI CTYRAG 2 cut(s) 2063, 2270
SsiI CCGC 6 cut(s) 12, 14, 552, 815, 1932, 2225
SstI GAGCTC 1 cut(s) 1037
StyD4I CCNGG 1 cut(s) 2196
StyI CCWWGG 3 cut(s) 381, 1020, 1421
TaaI ACNGT 5 cut(s) 235, 634, 804, 1818, 2087
TaiI ACGT 1 cut(s) 258
TaqI TCGA 2 cut(s) 1893, 2186
TaqII GACCGA 1 cut(s) 1928
TatI WGTACW 1 cut(s) 1906
TauI GCSGC 2 cut(s) 14, 1935
TfiI GAWTC 5 cut(s) 238, 1087, 1302, 1845, 2255
Tru1I TTAA 8 cut(s) 66, 89, 809, 1802, 2064, 2207, 2402, 2419
Tru9I TTAA 8 cut(s) 66, 89, 809, 1802, 2064, 2207, 2402, 2419
TscAI CASTG 2 cut(s) 981, 1082
TseFI GTSAC 2 cut(s) 628, 1771
TseI GCWGC 2 cut(s) 1313, 2248
Tsp45I GTSAC 2 cut(s) 628, 1771
TspGWI ACGGA 2 cut(s) 1551, 1578
TspRI CASTG 2 cut(s) 981, 1082
Tth111I GACNNNGTC 1 cut(s) 593
Van91I CCANNNNNTGG 2 cut(s) 157, 1584
Vha464I CTTAAG 1 cut(s) 2063
VpaK11BI GGWCC 1 cut(s) 2350
XapI RAATTY 7 cut(s) 85, 605, 915, 1359, 1702, 1807, 1968
XbaI TCTAGA 2 cut(s) 1348, 1990
XceI RCATGY 2 cut(s) 207, 1672
XmiI GTMKAC 2 cut(s) 290, 796
XmnI GAANNNNTTC 1 cut(s) 1206
Zsp2I ATGCAT 1 cut(s) 1981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.