MD02G1166300.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000039
Physical Location & Seq
Forward (+)
14253360 .. 14256557
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1166300.v1.1.491

Sequence Viewer

Length: 2439 bp
ATGTCTGTGGCTTCTGTCGGAATGGGTGCTCTGAGAATGGCATTTCAAAAACTGTTTGATGCTGTAAAATCAGTGCAGGAGGAGACCACAGTGTTCGAGCACCTCCTTGGAGACATCAAATCCACTCTGGACTCTCTACAGCCACTCATCAAAGACATCGAAAAATATAACTCGAAAGAGGGACTGGAATATATTGCAATACAAATGGAGGAGGGAGCAAATCTTGTTCAAAAGTGCAGCAAAGTTGGCGTGTGGAGAAAATACAAATACACCAACAAGCTTCATCAACTGGACAAATCTCTTCGAAGAGTGTTTGATCTACTCAAAGTGCAGAGCATAATGGATGCGAGGGAGACATTGGTTTCTTTGAGGAATATCGAGATGGCGCTCTCTGGAATAGAAGAGAATATGGTGATCAAAATTAATCACCCTGAAAAGGTTGAATGTTTGTCTGCTGTGCCTGAACCACCATGCATTACAGTCGGGTTGGATTTGCCGTTGAAGGAGTTGAAGATGAAGCTTCTCAAGGATGAAAAGGTGTCGATGCTTGTGCTGACTGCTGCTGGAGGATGTGGGAAAACCACCTTAGCTAAAATGTTTTGTCAAGATCAAGAAGTCAAAGATACATTCAAGAACAACATCTTCTTTGTCAATGTCTCGAAAAATCCCAACTTGGACCTTATTGTACATGAACTATATCAACGGAAGGGGTCCGAGCTGCCTGCTTTCCAAAACGAATTCATGGCAGTTAAATGGTTGCAAAATTTTTTGAAGGAATCAAGACACAACCCTCTGTTGTTTGTCCTGGATGATGTTTGGTCAGGATCAGAGTCCCTTCTTGATAGGTTTGATCAATTCAAGTTTTCAAATTACAAGATTTTGGTCACATCAAGATTTGCATTTTCGAGATTTGGTTCTCCATACCATTTGGCATTATTGAATGATGAGGATGCAATGACTCTTTTTCACCATTCAGCATCCTTGGAGGATAAAAGCTCTTATACTTCCGAAGATCTTTCGAGAAAGATTATCAAGTGCTGTAAGGGATGCCCACTTGCCATTGCATTGGTCGGGAGGTCACTTCGCAGCCAGCCTATAGAAATCTGGCAGAAAAGAGTGCTGGAATGGTGCAAAGGTTCATCTATTCTTGATTCTGACAAGGATCTGCTTGCTTGCCTCCAAAGCAGCTTAGATGCCTTGGATAAAGAAAAGACAATCATCAAGGAATGTTTCCTCGATCTAGGTTCATTTCCCGAGGACCAAAGAATTGCTGCTGCCGTCCTCATTGACATGTGGGCGGAGTTATATGGTCTAGATAAAGAAATTCTGACCATTGCCAACCTTCACGAACTCACCACTCGAAGTTTGGCAAATCTTGTTGTCACAAGGCATGAGAGGGAGGCGGACGGCTATTACACTGAACACTTTGTCACCCAGCATGAGATGCTTAGAGAATTGGCGATCCATCAGGCAAGTCAAGACCCAATAGACCAGAGGAAAAGACTAGTTATAAACATATGTGGGGATAATCTTCCCAAGTGGTTGACGGAACAAAAGTATCAAGCTTTCAAGGCTCGACTATTATCTATCTCCACAGATGGAGCCTTCTCAGCAAAATGGCCAAACATGCAAGTACCCGAAGCAGAGGTTCTAGTTCTGAATTTTCAAACAAACAACTATGCCTTACCTGAGTTTGTGGTGAATATGGATAAACTAAAGGTTCTAATAATCACAAATTACGGTTTGTCACCTGCTGAAGTAGATAATTTTGAACTACTTGGCTCGTTATCAAATCTTAAGAGAATTAGATTAGAACGCATTTCAATTCCCTCCATAAGCAAGGTCCTCAAACAGTTGAAAAGTTTGCAAAAGATGTCTCTTTTCTTGTGCGATATCAGTCAAGGTTTCGTCCAAATTTTAGATGCACTGCCAAATCTAGTGGAATTGAACATAGATTATTGCCACGACCTGGTGGAACTTCCTGCCAACCTTGGTGACCTTATTCACCTTACAAAGCTTAGCATCACCAACTGTCATAAGCTATCCACGTTGCCTGAGGACATCGGAAAGTTAGTCAATTTAGAAGTGTTGAGGCTTAGGTCATGTGCAGACTTGTTAGAGTTGCCACGCTCGATTAGGAACCTTAATAATGTAAAACTTTTCGACATATCTGATTGCTTTAGCATAAAGGAGTTGCCCGAGGATATTGGTGCAATGCGCAGTTTGGAAAAGATCAACGTTAGCCAGTGCTCAGGATTGAAAGAGCTGCCTGCTTCTGTTTTGGATCTTGAGCAGTTGAATCAAGTGATATGCGACGAGGAGACGGAAACTTTATGGGAACCGTTCTTACCATATTTGACAGACATACACATAAAGGTGATCAAAGAAGTTATGAACCTAAATTGGCTCAACAAGCTTCCTGGAGGCCATGGTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

813

Amino Acids

92.23

Weight (kDa)

5.7

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 130 7.2e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 167 - 326 2.9e-14 NB-ARC domain
AAA_22 PF13401 181 - 272 6e-06 AAA domain
LRR_14 PF23598 663 - 719 1.5e-06 Leucine-rich repeat region
LRR_14 PF23598 713 - 776 1.5e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1511
AarI CACCTGC 1 cut(s) 1759
Acc16I TGCGCA 1 cut(s) 2219
Acc36I ACCTGC 1 cut(s) 1759
AccB7I CCANNNNNTGG 1 cut(s) 1969
AciI CCGC 2 cut(s) 1298, 1403
AclI AACGTT 1 cut(s) 2238
AclWI GGATC 4 cut(s) 832, 1170, 1456, 2292
AcoI YGGCCR 1 cut(s) 1619
AcsI RAATTY 5 cut(s) 737, 763, 1323, 1660, 1914
AcuI CTGAAG 1 cut(s) 1776
AfaI GTAC 2 cut(s) 687, 1635
AfiI CCNNNNNNNGG 2 cut(s) 436, 1969
AflII CTTAAG 1 cut(s) 1796
AflIII ACRYGT 1 cut(s) 1290
AhlI ACTAGT 1 cut(s) 1504
AjnI CCWGG 3 cut(s) 804, 1968, 2419
Alw21I GWGCWC 3 cut(s) 31, 102, 2252
Alw26I GTCTC 6 cut(s) 77, 105, 347, 661, 1881, 2315
AlwI GGATC 4 cut(s) 832, 1170, 1456, 2292
Ama87I CYCGRG 2 cut(s) 1253, 2198
AoxI GGCC 2 cut(s) 1619, 2425
ApeKI GCWGC 8 cut(s) 237, 560, 718, 1086, 1185, 1271, 1274, 2266
ApoI RAATTY 5 cut(s) 737, 763, 1323, 1660, 1914
AseI ATTAAT 1 cut(s) 423
AspLEI GCGC 2 cut(s) 388, 2220
AspS9I GGNCC 4 cut(s) 676, 711, 1258, 1843
AsuII TTCGAA 1 cut(s) 304
AvaI CYCGRG 2 cut(s) 1253, 2198
AvaII GGWCC 4 cut(s) 676, 711, 1258, 1843
AxyI CCTNAGG 1 cut(s) 2055
BaeI ACNNNNGTAYC 2 cut(s) 1541, 1574
BalI TGGCCA 1 cut(s) 1621
Bbv12I GWGCWC 3 cut(s) 31, 102, 2252
BbvI GCAGC 8 cut(s) 249, 547, 705, 1098, 1197, 1258, 1261, 2253
BccI CCATC 3 cut(s) 376, 1473, 1592
BceAI ACGGC 3 cut(s) 481, 1262, 1423
BcgI CGANNNNNNTGC 6 cut(s) 463, 497, 532, 566, 704, 738
BciT130I CCWGG 3 cut(s) 806, 1970, 2421
BclI TGATCA 3 cut(s) 414, 850, 2379
BcoDI GTCTC 6 cut(s) 77, 105, 347, 661, 1881, 2315
BcuI ACTAGT 1 cut(s) 1504
BfaI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1937
BfmI CTRYAG 2 cut(s) 137, 1095
BfoI RGCGCY 1 cut(s) 389
BfrI CTTAAG 1 cut(s) 1796
BfuAI ACCTGC 1 cut(s) 1759
BglII AGATCT 1 cut(s) 1012
BisI GCNGC 8 cut(s) 238, 561, 719, 1087, 1186, 1272, 1275, 2267
BlpI GCTNAGC 1 cut(s) 2018
BlsI GCNGC 8 cut(s) 239, 562, 720, 1088, 1187, 1273, 1276, 2268
Bme1390I CCNGG 3 cut(s) 806, 1970, 2421
Bme18I GGWCC 4 cut(s) 676, 711, 1258, 1843
BmeT110I CYCGRG 2 cut(s) 1253, 2198
BmgT120I GGNCC 4 cut(s) 676, 711, 1258, 1843
BmiI GGNNCC 4 cut(s) 712, 1603, 2141, 2340
BmrFI CCNGG 3 cut(s) 806, 1970, 2421
BpmI CTGGAG 1 cut(s) 585
Bpu10I CCTNAGC 3 cut(s) 586, 2096, 2251
Bpu1102I GCTNAGC 1 cut(s) 2018
Bpu14I TTCGAA 1 cut(s) 304
BpuEI CTTGAG 2 cut(s) 509, 2309
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 7 cut(s) 106, 981, 1197, 1254, 1990, 2199, 2428
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 1969
Bse1I ACTGG 3 cut(s) 189, 294, 2245
Bse21I CCTNAGG 1 cut(s) 2055
Bse3DI GCAATG 4 cut(s) 960, 1059, 1332, 2220
BseBI CCWGG 3 cut(s) 806, 1970, 2421
BseDI CCNNGG 7 cut(s) 106, 981, 1197, 1254, 1990, 2199, 2428
BseGI GGATG 7 cut(s) 349, 535, 575, 814, 955, 977, 1052
BseLI CCNNNNNNNGG 2 cut(s) 436, 1969
BseMI GCAATG 4 cut(s) 960, 1059, 1332, 2220
BseMII CTCAG 5 cut(s) 23, 1623, 1680, 2046, 2265
BseNI ACTGG 3 cut(s) 189, 294, 2245
BseRI GAGGAG 3 cut(s) 95, 224, 2333
BseXI GCAGC 8 cut(s) 249, 547, 705, 1098, 1197, 1258, 1261, 2253
BseYI CCCAGC 1 cut(s) 1434
BsgI GTGCAG 4 cut(s) 95, 256, 350, 2127
BshFI GGCC 2 cut(s) 1621, 2427
BsiHKAI GWGCWC 3 cut(s) 31, 102, 2252
BsiHKCI CYCGRG 2 cut(s) 1253, 2198
BslFI GGGAC 2 cut(s) 195, 817
BslI CCNNNNNNNGG 2 cut(s) 436, 1969
BsmAI GTCTC 6 cut(s) 77, 105, 347, 661, 1881, 2315
BsmBI CGTCTC 1 cut(s) 2315
BsmFI GGGAC 2 cut(s) 195, 817
BsnI GGCC 2 cut(s) 1621, 2427
Bso31I GGTCTC 1 cut(s) 77
BsoBI CYCGRG 2 cut(s) 1253, 2198
Bsp119I TTCGAA 1 cut(s) 304
Bsp1286I GDGCHC 3 cut(s) 31, 102, 2252
Bsp1407I TGTACA 1 cut(s) 685
Bsp1720I GCTNAGC 1 cut(s) 2018
Bsp19I CCATGG 1 cut(s) 2428
BspACI CCGC 2 cut(s) 1298, 1403
BspANI GGCC 2 cut(s) 1621, 2427
BspCNI CTCAG 5 cut(s) 24, 1622, 1681, 2047, 2264
BspLI GGNNCC 4 cut(s) 712, 1603, 2141, 2340
BspMI ACCTGC 1 cut(s) 1759
BspPI GGATC 4 cut(s) 832, 1170, 1456, 2292
BspT104I TTCGAA 1 cut(s) 304
BspTI CTTAAG 1 cut(s) 1796
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 4 cut(s) 960, 1059, 1332, 2220
BsrGI TGTACA 1 cut(s) 685
BsrI ACTGG 3 cut(s) 189, 294, 2245
BssECI CCNNGG 7 cut(s) 106, 981, 1197, 1254, 1990, 2199, 2428
BssT1I CCWWGG 5 cut(s) 106, 981, 1197, 1990, 2428
Bst2UI CCWGG 3 cut(s) 806, 1970, 2421
Bst4CI ACNGT 7 cut(s) 54, 91, 481, 1742, 1854, 2033, 2343
Bst6I CTCTTC 3 cut(s) 301, 306, 396
BstAFI CTTAAG 1 cut(s) 1796
BstAPI GCANNNNNTGC 1 cut(s) 1444
BstAUI TGTACA 1 cut(s) 685
BstBI TTCGAA 1 cut(s) 304
BstC8I GCNNGC 5 cut(s) 723, 1091, 1170, 1174, 2271
BstDSI CCRYGG 1 cut(s) 2428
BstEII GGTNACC 1 cut(s) 1994
BstF5I GGATG 7 cut(s) 349, 535, 575, 814, 955, 977, 1052
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 2 cut(s) 388, 2220
BstMAI GTCTC 6 cut(s) 77, 105, 347, 661, 1881, 2315
BstMWI GCNNNNNNNGC 7 cut(s) 246, 1182, 1444, 1571, 1610, 1627, 2413
BstNI CCWGG 3 cut(s) 806, 1970, 2421
BstNSI RCATGY 2 cut(s) 1294, 1630
BstPI GGTNACC 1 cut(s) 1994
BstSCI CCNGG 3 cut(s) 804, 1968, 2419
BstSFI CTRYAG 2 cut(s) 137, 1095
BstV1I GCAGC 8 cut(s) 249, 547, 705, 1098, 1197, 1258, 1261, 2253
BstX2I RGATCY 3 cut(s) 1012, 1162, 2284
BstXI CCANNNNNNTGG 1 cut(s) 1066
BstYI RGATCY 3 cut(s) 1012, 1162, 2284
Bsu36I CCTNAGG 1 cut(s) 2055
BsuRI GGCC 2 cut(s) 1621, 2427
BtgI CCRYGG 1 cut(s) 2428
BtsCI GGATG 7 cut(s) 349, 535, 575, 814, 955, 977, 1052
BtsI GCAGTG 1 cut(s) 1925
BtsIMutI CAGTG 5 cut(s) 78, 96, 1416, 1925, 2252
BveI ACCTGC 1 cut(s) 1759
Cac8I GCNNGC 5 cut(s) 723, 1091, 1170, 1174, 2271
CfoI GCGC 2 cut(s) 388, 2220
Cfr13I GGNCC 4 cut(s) 676, 711, 1258, 1843
CsiI ACCWGGT 1 cut(s) 1968
Csp6I GTAC 2 cut(s) 686, 1634
CspCI CAANNNNNGTGG 2 cut(s) 1920, 1955
CviAII CATG 9 cut(s) 471, 689, 742, 1291, 1391, 1439, 1627, 2103, 2429
CviQI GTAC 2 cut(s) 686, 1634
EaeI YGGCCR 1 cut(s) 1619
Eam1104I CTCTTC 3 cut(s) 301, 306, 396
EarI CTCTTC 3 cut(s) 301, 306, 396
EciI GGCGGA 2 cut(s) 1313, 1418
Eco130I CCWWGG 5 cut(s) 106, 981, 1197, 1990, 2428
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 1894
Eco47I GGWCC 4 cut(s) 676, 711, 1258, 1843
Eco57I CTGAAG 1 cut(s) 1776
Eco81I CCTNAGG 1 cut(s) 2055
Eco88I CYCGRG 2 cut(s) 1253, 2198
Eco91I GGTNACC 1 cut(s) 1994
EcoO109I RGGNCCY 1 cut(s) 1843
EcoO65I GGTNACC 1 cut(s) 1994
EcoRI GAATTC 1 cut(s) 737
EcoRII CCWGG 3 cut(s) 804, 1968, 2419
EcoRV GATATC 1 cut(s) 1894
EcoT14I CCWWGG 5 cut(s) 106, 981, 1197, 1990, 2428
EcoT22I ATGCAT 1 cut(s) 476
ErhI CCWWGG 5 cut(s) 106, 981, 1197, 1990, 2428
Esp3I CGTCTC 1 cut(s) 2315
FaeI CATG 9 cut(s) 474, 692, 745, 1294, 1394, 1442, 1630, 2106, 2432
FaqI GGGAC 2 cut(s) 195, 817
FatI CATG 9 cut(s) 470, 688, 741, 1290, 1390, 1438, 1626, 2102, 2428
FauNDI CATATG 1 cut(s) 1517
FbaI TGATCA 3 cut(s) 414, 850, 2379
Fnu4HI GCNGC 8 cut(s) 238, 561, 719, 1087, 1186, 1272, 1275, 2267
FokI GGATG 7 cut(s) 356, 542, 582, 821, 962, 964, 1059
Fsp4HI GCNGC 8 cut(s) 238, 561, 719, 1087, 1186, 1272, 1275, 2267
FspBI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1937
FspI TGCGCA 1 cut(s) 2219
GlaI GCGC 2 cut(s) 387, 2219
GluI GCNGC 8 cut(s) 238, 561, 719, 1087, 1186, 1272, 1275, 2267
GsaI CCCAGC 1 cut(s) 1438
GsuI CTGGAG 1 cut(s) 585
HaeII RGCGCY 1 cut(s) 389
HaeIII GGCC 2 cut(s) 1621, 2427
HhaI GCGC 2 cut(s) 388, 2220
Hin1II CATG 9 cut(s) 474, 692, 745, 1294, 1394, 1442, 1630, 2106, 2432
Hin6I GCGC 2 cut(s) 386, 2218
HinP1I GCGC 2 cut(s) 386, 2218
HincII GTYRAC 1 cut(s) 1545
HindII GTYRAC 1 cut(s) 1545
HindIII AAGCTT 5 cut(s) 278, 518, 1563, 2015, 2414
HinfI GANTC 6 cut(s) 131, 776, 830, 958, 1151, 2299
Hpy166II GTNNAC 1 cut(s) 1545
Hpy8I GTNNAC 1 cut(s) 1545
Hpy99I CGWCG 1 cut(s) 2318
HpyAV CCTTC 6 cut(s) 496, 700, 766, 845, 1352, 1615
HpyCH4III ACNGT 7 cut(s) 54, 91, 481, 1742, 1854, 2033, 2343
HpyCH4IV ACGT 2 cut(s) 2048, 2238
HpyF10VI GCNNNNNNNGC 7 cut(s) 246, 1182, 1444, 1571, 1610, 1627, 2413
HpySE526I ACGT 2 cut(s) 2048, 2238
Hsp92II CATG 9 cut(s) 474, 692, 745, 1294, 1394, 1442, 1630, 2106, 2432
HspAI GCGC 2 cut(s) 386, 2218
Ksp22I TGATCA 3 cut(s) 414, 850, 2379
LmnI GCTCC 2 cut(s) 215, 1601
Lsp1109I GCAGC 8 cut(s) 249, 547, 705, 1098, 1197, 1258, 1261, 2253
MabI ACCWGGT 1 cut(s) 1968
MaeI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1937
MaeII ACGT 2 cut(s) 2048, 2238
MaeIII GTNAC 6 cut(s) 883, 1077, 1381, 1429, 1746, 1994
MboII GAAGA 7 cut(s) 293, 318, 413, 523, 634, 1022, 1523
MflI RGATCY 3 cut(s) 1012, 1162, 2284
MhlI GDGCHC 3 cut(s) 31, 102, 2252
MlsI TGGCCA 1 cut(s) 1621
MluNI TGGCCA 1 cut(s) 1621
MlyI GAGTC 3 cut(s) 125, 839, 952
MmeI TCCRAC 1 cut(s) 468
Mox20I TGGCCA 1 cut(s) 1621
Mph1103I ATGCAT 1 cut(s) 476
MscI TGGCCA 1 cut(s) 1621
MseI TTAA 4 cut(s) 423, 750, 1797, 2145
MslI CAYNNNNRTG 2 cut(s) 1289, 2375
Msp20I TGGCCA 1 cut(s) 1621
MspCI CTTAAG 1 cut(s) 1796
MspR9I CCNGG 3 cut(s) 806, 1970, 2421
MvaI CCWGG 3 cut(s) 806, 1970, 2421
MwoI GCNNNNNNNGC 7 cut(s) 246, 1182, 1444, 1571, 1610, 1627, 2413
NcoI CCATGG 1 cut(s) 2428
NdeI CATATG 1 cut(s) 1517
NlaIII CATG 9 cut(s) 474, 692, 745, 1294, 1394, 1442, 1630, 2106, 2432
NlaIV GGNNCC 4 cut(s) 712, 1603, 2141, 2340
NmuCI GTSAC 6 cut(s) 883, 1077, 1381, 1429, 1746, 1994
NsbI TGCGCA 1 cut(s) 2219
NsiI ATGCAT 1 cut(s) 476
NspI RCATGY 2 cut(s) 1294, 1630
NspV TTCGAA 1 cut(s) 304
PaqCI CACCTGC 1 cut(s) 1759
PciI ACATGT 1 cut(s) 1290
PfeI GAWTC 3 cut(s) 776, 1151, 2299
PflMI CCANNNNNTGG 1 cut(s) 1969
PfoI TCCNGGA 2 cut(s) 804, 2419
PkrI GCNGC 8 cut(s) 239, 562, 720, 1088, 1187, 1273, 1276, 2268
PleI GAGTC 3 cut(s) 125, 838, 952
PpsI GAGTC 3 cut(s) 125, 838, 952
PpuMI RGGWCCY 1 cut(s) 1843
PscI ACATGT 1 cut(s) 1290
PshBI ATTAAT 1 cut(s) 423
PsiI TTATAA 1 cut(s) 1511
Psp1406I AACGTT 1 cut(s) 2238
Psp5II RGGWCCY 1 cut(s) 1843
Psp6I CCWGG 3 cut(s) 804, 1968, 2419
PspEI GGTNACC 1 cut(s) 1994
PspFI CCCAGC 1 cut(s) 1434
PspGI CCWGG 3 cut(s) 804, 1968, 2419
PspN4I GGNNCC 4 cut(s) 712, 1603, 2141, 2340
PspPI GGNCC 4 cut(s) 676, 711, 1258, 1843
PspPPI RGGWCCY 1 cut(s) 1843
PsrI GAACNNNNNNTAC 2 cut(s) 2331, 2363
PsuI RGATCY 3 cut(s) 1012, 1162, 2284
RsaI GTAC 2 cut(s) 687, 1635
RsaNI GTAC 2 cut(s) 686, 1634
RseI CAYNNNNRTG 2 cut(s) 1289, 2375
SaqAI TTAA 4 cut(s) 423, 750, 1797, 2145
SatI GCNGC 8 cut(s) 238, 561, 719, 1087, 1186, 1272, 1275, 2267
Sau96I GGNCC 4 cut(s) 676, 711, 1258, 1843
SchI GAGTC 3 cut(s) 125, 839, 952
ScrFI CCNGG 3 cut(s) 806, 1970, 2421
SduI GDGCHC 3 cut(s) 31, 102, 2252
SexAI ACCWGGT 1 cut(s) 1968
SfcI CTRYAG 2 cut(s) 137, 1095
SfuI TTCGAA 1 cut(s) 304
SinI GGWCC 4 cut(s) 676, 711, 1258, 1843
SmiMI CAYNNNNRTG 2 cut(s) 1289, 2375
SmlI CTYRAG 3 cut(s) 524, 1796, 2288
SmoI CTYRAG 3 cut(s) 524, 1796, 2288
SpeI ACTAGT 1 cut(s) 1504
SsiI CCGC 2 cut(s) 1298, 1403
SspMI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1937
StyD4I CCNGG 3 cut(s) 804, 1968, 2419
StyI CCWWGG 5 cut(s) 106, 981, 1197, 1990, 2428
TaaI ACNGT 7 cut(s) 54, 91, 481, 1742, 1854, 2033, 2343
TaiI ACGT 2 cut(s) 2051, 2241
TatI WGTACW 1 cut(s) 685
TfiI GAWTC 3 cut(s) 776, 1151, 2299
Tru1I TTAA 4 cut(s) 423, 750, 1797, 2145
Tru9I TTAA 4 cut(s) 423, 750, 1797, 2145
TscAI CASTG 5 cut(s) 78, 96, 1423, 1932, 2252
TseFI GTSAC 6 cut(s) 883, 1077, 1381, 1429, 1746, 1994
TseI GCWGC 8 cut(s) 237, 560, 718, 1086, 1185, 1271, 1274, 2266
Tsp45I GTSAC 6 cut(s) 883, 1077, 1381, 1429, 1746, 1994
TspDTI ATGAA 8 cut(s) 272, 530, 546, 705, 730, 1128, 1236, 2408
TspGWI ACGGA 3 cut(s) 718, 1562, 2339
TspRI CASTG 5 cut(s) 78, 96, 1423, 1932, 2252
Van91I CCANNNNNTGG 1 cut(s) 1969
Vha464I CTTAAG 1 cut(s) 1796
VpaK11BI GGWCC 4 cut(s) 676, 711, 1258, 1843
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 5 cut(s) 737, 763, 1323, 1660, 1914
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 2 cut(s) 1294, 1630
XcmI CCANNNNNNNNNTGG 1 cut(s) 1363
XspI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1937
Zsp2I ATGCAT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.