RchiOBHm_Chr2g0170631

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000039
Physical Location & Seq
Forward (+)
84523308 .. 84527350
4043 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53813

Sequence Viewer

Length: 2481 bp
ATGGCTATGGTGGCGGACTTTGTACTAGAGAAAGCATTCGGAGCACTGTATGCTGGCGTTGAAGACCTGATCATCAAGAATTTCATTTTTAAGTCTCTCTTCAACAAAGTAAAATCCAGGCTCGATGATTGGGCACCACTGATTAAGGAGATGGCCCAATACACCCAGAAAATGGATCGCCCAGAGAAGGATCTGGAAGCTTTAAAGGGAGTCATGCAGACGGGCCAAGAGCTCGTCAACGAGTGCTCAAAGATTACGCGATGGAGCCCCTTCAAAAAGTACAAGTACGCCAAGAAACTTCAGGAATGGCAGGAATCTCTTCAAAGCCCGTTGGACAGACTGAAACTGCAAGACACAATGAATATAATGGAGATCAGGGTCGTGGTTGATGAAATGCAACGTGACATGCTGAAACAACAATCTGCAGAAAGTTCAAGTAGTACACGACTTGAACTTCCACCGCTTCCAAAGCTTATAGTTGGACTGGATGGTCCTTTGAATGATTTGAAGGAAAAGCTTCTTGCGGATGATGGGGTGTCGAGGCTTGTGCTCACTGCTCCTGGAGGCTGTGGGAAAACCACTCTCGCCACAATGTTTTGTGAAGATGAAGAGGTCAAAGGTATATTCAAGAAAAATATTATCTTCATCACTGTTTCACAAAAAGGCAATTTAGAACTTGTTGTGAAGCGACTTTGTGAATACTTGACGAGTGTGAGGTCTCCGGTTCCTACTTTCCAAAACAAAGTACATATGCTTCAATGGATGCAAACATTTTTCAAGTCTTCCCAAAATCCTTTGCTATTGGTCCTCGATGATATTTGGTCCGGATCAGAATCCATTCTTCATAGCTTTCAATTTGAATTGTCCAATTACAAGATTTTAGTAACATCAAGATCTGAATTTCCAAATTTTGGTTCTTCATATCATTTAAAATTATTGGATGATGACAAAGCTATGGAACTTTTTTATCACTCAACATCATTGGGAGATAAGTGCTCTCCTGATCAAGAAGAACTCTCAAGAAAGATAGTAAAGCGTTGCAACGGATTTCCACTAGCTATTACAGTGGTTGGCAGATCACTTTCTGGGAAACCTATAGAGATCTGGAGACAGAGAGAATCTGAATGGTCTAAAGATTCTTCAATTCTAGATTCTGATCCTGAGACGGAATTGTTTCTTTGTCTCCAAAGCAGCTTAGATGCTCTGGATAAAAGACTTGCAATTGTCAAAGATTGTTACTTGGACCTGGGATCTTTTCCTGAAGACAAAGAAATCCGAGTTGATGACCTCATTGATATGTGGGCGGAGTTATATGATATAGACGATGACAGTGTGTGCTTTGCAAATATCTATGAACTTGCCAATCGAAATCTGGCTAATCTGGTTGACAAAAGGGACAGCTATATGAAGATGGAGGGGGACGATTTCTACGGTGAACGGTTTGTCACCCAACATGATTTGCTTAGAGAGCTAGCTATTTACAATGCTAAGCTAGACCCGGTAGAGCAAAGACAAAGAATGAACATAGAAATAAGGGGAAACAAAATCCCCAAGTGGTGGAGAGAACAAAAATGTCACCCTATCAAGGCTCGCTTGTTATCTATTACTACAACTACTGACTTCGATATGGAATGGCCCAACTTGCAACTACCAGAAGCAGAAGTTTTTGTCTTGCATTTTCAGAGCCCAAGTAGTTACAGCTTGCCCGCGTTTGTACAGAAAATGGATAAATTGAAGGTACTGGTAATAGACGGCGCTGAGGAATTAATAAATTTTGAACTACTCGGTTTGTTACCAAATCTAAAGAGAATCAGTTTAATTCGTCTCCAAATTGGTTTCTTGACCCACAAGCCCATACAACTGAAAAGTCTAAAGAAGATATCTTTCGCGTCATGTGGCGCGGAGGGTATCGACGAAGGTTTTTTCAGAATGTCGGATGCATTTCCGAATTTGGAGGAATTATACATTCAGTTCGGAGTAAGGCTGGTTTCTGATTTCTTCCAACAGTTAAACGGTAGAGAATTTGTTTTGGAGTGCTGCAATCTGATTCATTTAAAGAAGATTAGTATCGTCAGAGGTACTGGGCTAGATCACTTGCCCGAAGAAATCGGAAATTTGGTCAACTTAGAAGTACTGAGGCTAAGGTCTTGTTTGTATCTTTCAAAGCTTCCCAGTTCCATCAGCAACCTCAAAAAGTTAAACTTCCTTGACATATATGGATGCTGGAGCATTGAGTTGCCTAAAAACATTGGTGAATTGAGCAGTTTAAGAAAGTTGGACATGAGGTGTTGCGACGAAGTGAAAGAGCTGCCTCCATCAGTTTCGGATCTTCATCAGTTAGAGGAAGTGATATGCAACCGTGAAGACCAAGGCAGTTTATGGGAACCCTTTCGACCCACTCTCACAAACCTGCGCGTGGTGGTGATTCCTCGAACCCAACCAAATTGGTTGGAAACTTTCAGCGACGGTTGGAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

826

Amino Acids

94.98

Weight (kDa)

5.38

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 135 1.3e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 165 - 323 7.5e-15 NB-ARC domain
AAA_22 PF13401 182 - 273 5.9e-06 AAA domain
LRR_14 PF23598 679 - 804 4e-17 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 489
Acc36I ACCTGC 1 cut(s) 2418
AccB1I GGYRCC 1 cut(s) 133
AccB7I CCANNNNNTGG 3 cut(s) 172, 911, 1557
AccII CGCG 5 cut(s) 259, 1709, 1889, 1901, 2415
AccIII TCCGGA 1 cut(s) 824
AciI CCGC 6 cut(s) 14, 461, 524, 1304, 1707, 1901
AclWI GGATC 6 cut(s) 183, 198, 835, 1151, 1258, 2334
AcsI RAATTY 7 cut(s) 79, 899, 907, 1771, 1948, 2021, 2113
AcuI CTGAAG 2 cut(s) 284, 1281
AfaI GTAC 9 cut(s) 24, 281, 287, 442, 747, 1716, 1740, 2080, 2133
AfiI CCNNNNNNNGG 6 cut(s) 172, 187, 911, 1557, 1585, 2416
AjnI CCWGG 3 cut(s) 116, 559, 1245
AjuI GAANNNNNNNTTGG 2 cut(s) 898, 930
Alw21I GWGCWC 5 cut(s) 46, 234, 248, 552, 998
Alw26I GTCTC 6 cut(s) 99, 723, 1102, 1157, 1187, 1829
AlwI GGATC 6 cut(s) 183, 198, 835, 1151, 1258, 2334
Aor13HI TCCGGA 1 cut(s) 824
AoxI GGCC 3 cut(s) 153, 223, 1634
ApeKI GCWGC 4 cut(s) 1191, 2037, 2308, 2475
ApoI RAATTY 7 cut(s) 79, 899, 907, 1771, 1948, 2021, 2113
AseI ATTAAT 1 cut(s) 1766
Asp700I GAANNNNTTC 6 cut(s) 35, 318, 516, 837, 1173, 2388
AspLEI GCGC 3 cut(s) 1757, 1901, 2415
AspS9I GGNCC 7 cut(s) 154, 223, 491, 805, 822, 1243, 1635
AsuC2I CCSGG 1 cut(s) 1499
AsuHPI GGTGA 5 cut(s) 1438, 1445, 1568, 2264, 2434
AsuNHI GCTAGC 1 cut(s) 1471
AvaII GGWCC 4 cut(s) 491, 805, 822, 1243
BaeGI GKGCMC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 3 cut(s) 234, 269, 1688
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
BbsI GAAGAC 4 cut(s) 69, 774, 1269, 2370
Bbv12I GWGCWC 5 cut(s) 46, 234, 248, 552, 998
BbvCI CCTCAGC 1 cut(s) 1758
BbvI GCAGC 3 cut(s) 1203, 2024, 2295
BccI CCATC 7 cut(s) 145, 255, 482, 524, 1405, 2186, 2323
BceAI ACGGC 1 cut(s) 1768
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BciT130I CCWGG 3 cut(s) 118, 561, 1247
BclI TGATCA 2 cut(s) 69, 1003
BcnI CCSGG 1 cut(s) 1499
BcoDI GTCTC 6 cut(s) 99, 723, 1102, 1157, 1187, 1829
BfaI CTAG 7 cut(s) 26, 1055, 1148, 1472, 1493, 2087, 2479
BfmI CTRYAG 2 cut(s) 423, 1095
BfoI RGCGCY 1 cut(s) 1758
BfuAI ACCTGC 1 cut(s) 2418
BglII AGATCT 2 cut(s) 893, 1101
BisI GCNGC 4 cut(s) 1192, 2038, 2309, 2476
BlpI GCTNAGC 1 cut(s) 1488
BlsI GCNGC 4 cut(s) 1193, 2039, 2310, 2477
BmcAI AGTACT 1 cut(s) 2133
Bme1390I CCNGG 4 cut(s) 118, 561, 1247, 1499
Bme18I GGWCC 4 cut(s) 491, 805, 822, 1243
BmgT120I GGNCC 7 cut(s) 154, 223, 491, 805, 822, 1243, 1635
BmiI GGNNCC 4 cut(s) 135, 266, 726, 2385
BmrFI CCNGG 4 cut(s) 118, 561, 1247, 1499
BmrI ACTGGG 2 cut(s) 2091, 2166
BmsI GCATC 4 cut(s) 753, 1189, 1927, 2210
BmtI GCTAGC 1 cut(s) 1475
BmuI ACTGGG 2 cut(s) 2091, 2166
BpiI GAAGAC 4 cut(s) 69, 774, 1269, 2370
BpmI CTGGAG 3 cut(s) 582, 1126, 2245
Bpu10I CCTNAGC 2 cut(s) 1758, 2141
Bpu1102I GCTNAGC 1 cut(s) 1488
BpuEI CTTGAG 1 cut(s) 1003
BpuMI CCSGG 1 cut(s) 1499
BsaBI GATNNNNATC 4 cut(s) 832, 1155, 2066, 2331
BsaI GGTCTC 1 cut(s) 723
BsaJI CCNNGG 2 cut(s) 1246, 2368
BsaWI WCCGGW 2 cut(s) 721, 824
Bsc4I CCNNNNNNNGG 6 cut(s) 172, 187, 911, 1557, 1585, 2416
Bse1I ACTGG 4 cut(s) 489, 1746, 2086, 2172
Bse8I GATNNNNATC 4 cut(s) 832, 1155, 2066, 2331
BseAI TCCGGA 1 cut(s) 824
BseBI CCWGG 3 cut(s) 118, 561, 1247
BseDI CCNNGG 2 cut(s) 1246, 2368
BseGI GGATG 6 cut(s) 493, 532, 768, 946, 1942, 2225
BseJI GATNNNNATC 4 cut(s) 832, 1155, 2066, 2331
BseLI CCNNNNNNNGG 6 cut(s) 172, 187, 911, 1557, 1585, 2416
BseMII CTCAG 3 cut(s) 1152, 1749, 2126
BseNI ACTGG 4 cut(s) 489, 1746, 2086, 2172
BseSI GKGCMC 1 cut(s) 136
BseXI GCAGC 3 cut(s) 1203, 2024, 2295
Bsh1236I CGCG 5 cut(s) 259, 1709, 1889, 1901, 2415
BshFI GGCC 3 cut(s) 155, 225, 1636
BshNI GGYRCC 1 cut(s) 133
BsiHKAI GWGCWC 5 cut(s) 46, 234, 248, 552, 998
BsiSI CCGG 3 cut(s) 722, 825, 1499
BslFI GGGAC 2 cut(s) 1409, 1433
BslI CCNNNNNNNGG 6 cut(s) 172, 187, 911, 1557, 1585, 2416
BsmAI GTCTC 6 cut(s) 99, 723, 1102, 1157, 1187, 1829
BsmBI CGTCTC 2 cut(s) 1157, 1829
BsmFI GGGAC 2 cut(s) 1409, 1433
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 3 cut(s) 155, 225, 1636
Bso31I GGTCTC 1 cut(s) 723
Bsp1286I GDGCHC 8 cut(s) 46, 136, 234, 248, 269, 552, 998, 1688
Bsp13I TCCGGA 1 cut(s) 824
Bsp1407I TGTACA 1 cut(s) 1714
Bsp1720I GCTNAGC 1 cut(s) 1488
BspACI CCGC 6 cut(s) 14, 461, 524, 1304, 1707, 1901
BspANI GGCC 3 cut(s) 155, 225, 1636
BspCNI CTCAG 3 cut(s) 1153, 1750, 2127
BspEI TCCGGA 1 cut(s) 824
BspFNI CGCG 5 cut(s) 259, 1709, 1889, 1901, 2415
BspLI GGNNCC 4 cut(s) 135, 266, 726, 2385
BspMAI CTGCAG 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 2418
BspOI GCTAGC 1 cut(s) 1475
BspPI GGATC 6 cut(s) 183, 198, 835, 1151, 1258, 2334
BspT107I GGYRCC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 723
BsrGI TGTACA 1 cut(s) 1714
BsrI ACTGG 4 cut(s) 489, 1746, 2086, 2172
BssECI CCNNGG 2 cut(s) 1246, 2368
BssT1I CCWWGG 1 cut(s) 2368
Bst2UI CCWGG 3 cut(s) 118, 561, 1247
Bst6I CTCTTC 3 cut(s) 104, 324, 603
BstAPI GCANNNNNTGC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 1714
BstC8I GCNNGC 5 cut(s) 55, 1473, 1591, 1703, 1707
BstDEI CTNAG 8 cut(s) 1161, 1195, 1463, 1488, 1758, 2125, 2135, 2141
BstF5I GGATG 6 cut(s) 493, 532, 768, 946, 1942, 2225
BstFNI CGCG 5 cut(s) 259, 1709, 1889, 1901, 2415
BstH2I RGCGCY 1 cut(s) 1758
BstHHI GCGC 3 cut(s) 1757, 1901, 2415
BstMAI GTCTC 6 cut(s) 99, 723, 1102, 1157, 1187, 1829
BstMWI GCNNNNNNNGC 6 cut(s) 11, 41, 50, 469, 1468, 1642
BstNI CCWGG 3 cut(s) 118, 561, 1247
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 4 cut(s) 116, 559, 1245, 1497
BstSFI CTRYAG 2 cut(s) 423, 1095
BstSLI GKGCMC 1 cut(s) 136
BstUI CGCG 5 cut(s) 259, 1709, 1889, 1901, 2415
BstV1I GCAGC 3 cut(s) 1203, 2024, 2295
BstV2I GAAGAC 4 cut(s) 69, 774, 1269, 2370
BstX2I RGATCY 5 cut(s) 190, 893, 1101, 1250, 2326
BstYI RGATCY 5 cut(s) 190, 893, 1101, 1250, 2326
BsuRI GGCC 3 cut(s) 155, 225, 1636
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 6 cut(s) 493, 532, 768, 946, 1942, 2225
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 6 cut(s) 44, 137, 552, 648, 1071, 1336
BveI ACCTGC 1 cut(s) 2418
Cac8I GCNNGC 5 cut(s) 55, 1473, 1591, 1703, 1707
CfoI GCGC 3 cut(s) 1757, 1901, 2415
Cfr13I GGNCC 7 cut(s) 154, 223, 491, 805, 822, 1243, 1635
CseI GACGC 1 cut(s) 1878
Csp6I GTAC 9 cut(s) 23, 280, 286, 441, 746, 1715, 1739, 2079, 2132
CviAII CATG 5 cut(s) 214, 406, 1454, 1893, 2281
CviQI GTAC 9 cut(s) 23, 280, 286, 441, 746, 1715, 1739, 2079, 2132
DdeI CTNAG 8 cut(s) 1161, 1195, 1463, 1488, 1758, 2125, 2135, 2141
DraI TTTAAA 3 cut(s) 204, 930, 2055
DrdI GACNNNNNNGTC 1 cut(s) 489
DseDI GACNNNNNNGTC 1 cut(s) 489
Eam1104I CTCTTC 3 cut(s) 104, 324, 603
EarI CTCTTC 3 cut(s) 104, 324, 603
EciI GGCGGA 2 cut(s) 29, 1319
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 2368
Eco24I GRGCYC 3 cut(s) 234, 269, 1688
Eco31I GGTCTC 1 cut(s) 723
Eco32I GATATC 1 cut(s) 1881
Eco47I GGWCC 4 cut(s) 491, 805, 822, 1243
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 284, 1281
EcoICRI GAGCTC 1 cut(s) 232
EcoRII CCWGG 3 cut(s) 116, 559, 1245
EcoRV GATATC 1 cut(s) 1881
EcoT14I CCWWGG 1 cut(s) 2368
EcoT22I ATGCAT 1 cut(s) 1942
EcoT38I GRGCYC 3 cut(s) 234, 269, 1688
ErhI CCWWGG 1 cut(s) 2368
Esp3I CGTCTC 2 cut(s) 1157, 1829
FaeI CATG 5 cut(s) 217, 409, 1457, 1896, 2284
FalI AAGNNNNNCTT 6 cut(s) 83, 115, 1577, 1609, 2186, 2218
FaqI GGGAC 2 cut(s) 1409, 1433
FatI CATG 5 cut(s) 213, 405, 1453, 1892, 2280
FauI CCCGC 1 cut(s) 1714
FauNDI CATATG 1 cut(s) 750
FbaI TGATCA 2 cut(s) 69, 1003
Fnu4HI GCNGC 4 cut(s) 1192, 2038, 2309, 2476
FokI GGATG 6 cut(s) 500, 539, 775, 953, 1949, 2232
FriOI GRGCYC 3 cut(s) 234, 269, 1688
Fsp4HI GCNGC 4 cut(s) 1192, 2038, 2309, 2476
FspBI CTAG 7 cut(s) 26, 1055, 1148, 1472, 1493, 2087, 2479
GlaI GCGC 3 cut(s) 1756, 1900, 2414
GluI GCNGC 4 cut(s) 1192, 2038, 2309, 2476
GsuI CTGGAG 3 cut(s) 582, 1126, 2245
HaeII RGCGCY 1 cut(s) 1758
HaeIII GGCC 3 cut(s) 155, 225, 1636
HapII CCGG 3 cut(s) 722, 825, 1499
HgaI GACGC 1 cut(s) 1878
HhaI GCGC 3 cut(s) 1757, 1901, 2415
Hin1II CATG 5 cut(s) 217, 409, 1457, 1896, 2284
Hin6I GCGC 3 cut(s) 1755, 1899, 2413
HinP1I GCGC 3 cut(s) 1755, 1899, 2413
HincII GTYRAC 3 cut(s) 238, 1387, 2122
HindII GTYRAC 3 cut(s) 238, 1387, 2122
HindIII AAGCTT 4 cut(s) 198, 470, 515, 2165
HinfI GANTC 9 cut(s) 210, 314, 833, 1118, 1136, 1151, 1809, 2047, 2425
HpaII CCGG 3 cut(s) 722, 825, 1499
HphI GGTGA 5 cut(s) 1438, 1445, 1568, 2264, 2434
Hpy166II GTNNAC 5 cut(s) 238, 443, 1387, 1436, 2122
Hpy8I GTNNAC 5 cut(s) 238, 443, 1387, 1436, 2122
Hpy99I CGWCG 3 cut(s) 1916, 2297, 2468
HpyAV CCTTC 5 cut(s) 181, 280, 502, 1729, 1910
HpyCH4IV ACGT 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 41, 50, 469, 1468, 1642
HpyF3I CTNAG 8 cut(s) 1161, 1195, 1463, 1488, 1758, 2125, 2135, 2141
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 5 cut(s) 217, 409, 1457, 1896, 2284
HspAI GCGC 3 cut(s) 1755, 1899, 2413
Kpn2I TCCGGA 1 cut(s) 824
Ksp22I TGATCA 2 cut(s) 69, 1003
LmnI GCTCC 5 cut(s) 41, 264, 562, 2226, 2472
Lsp1109I GCAGC 3 cut(s) 1203, 2024, 2295
LweI GCATC 4 cut(s) 753, 1189, 1927, 2210
MaeI CTAG 7 cut(s) 26, 1055, 1148, 1472, 1493, 2087, 2479
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 7 cut(s) 401, 883, 1235, 1444, 1574, 1694, 1791
MfeI CAATTG 1 cut(s) 1221
MflI RGATCY 5 cut(s) 190, 893, 1101, 1250, 2326
MhlI GDGCHC 8 cut(s) 46, 136, 234, 248, 269, 552, 998, 1688
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 7 cut(s) 312, 460, 1914, 2026, 2256, 2430, 2450
Mph1103I ATGCAT 1 cut(s) 1942
MroI TCCGGA 1 cut(s) 824
MroXI GAANNNNTTC 6 cut(s) 35, 318, 516, 837, 1173, 2388
MslI CAYNNNNRTG 1 cut(s) 1295
MspI CCGG 3 cut(s) 722, 825, 1499
MspR9I CCNGG 4 cut(s) 118, 561, 1247, 1499
MunI CAATTG 1 cut(s) 1221
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 3 cut(s) 118, 561, 1247
MvnI CGCG 5 cut(s) 259, 1709, 1889, 1901, 2415
MwoI GCNNNNNNNGC 6 cut(s) 11, 41, 50, 469, 1468, 1642
NciI CCSGG 1 cut(s) 1499
NdeI CATATG 1 cut(s) 750
NheI GCTAGC 1 cut(s) 1471
NlaIII CATG 5 cut(s) 217, 409, 1457, 1896, 2284
NlaIV GGNNCC 4 cut(s) 135, 266, 726, 2385
NmuCI GTSAC 3 cut(s) 401, 1444, 1574
NsiI ATGCAT 1 cut(s) 1942
NspI RCATGY 1 cut(s) 409
PctI GAATGC 1 cut(s) 35
PdmI GAANNNNTTC 6 cut(s) 35, 318, 516, 837, 1173, 2388
PfeI GAWTC 8 cut(s) 314, 833, 1118, 1136, 1151, 1809, 2047, 2425
PflMI CCANNNNNTGG 3 cut(s) 172, 911, 1557
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 4 cut(s) 1193, 2039, 2310, 2477
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PshBI ATTAAT 1 cut(s) 1766
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 3 cut(s) 116, 559, 1245
PspGI CCWGG 3 cut(s) 116, 559, 1245
PspN4I GGNNCC 4 cut(s) 135, 266, 726, 2385
PspPI GGNCC 7 cut(s) 154, 223, 491, 805, 822, 1243, 1635
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 5 cut(s) 190, 893, 1101, 1250, 2326
RsaI GTAC 9 cut(s) 24, 281, 287, 442, 747, 1716, 1740, 2080, 2133
RsaNI GTAC 9 cut(s) 23, 280, 286, 441, 746, 1715, 1739, 2079, 2132
RseI CAYNNNNRTG 1 cut(s) 1295
SacI GAGCTC 1 cut(s) 234
SatI GCNGC 4 cut(s) 1192, 2038, 2309, 2476
Sau96I GGNCC 7 cut(s) 154, 223, 491, 805, 822, 1243, 1635
ScaI AGTACT 1 cut(s) 2133
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 4 cut(s) 118, 561, 1247, 1499
SduI GDGCHC 8 cut(s) 46, 136, 234, 248, 269, 552, 998, 1688
SfaNI GCATC 4 cut(s) 753, 1189, 1927, 2210
SfcI CTRYAG 2 cut(s) 423, 1095
SinI GGWCC 4 cut(s) 491, 805, 822, 1243
SmiMI CAYNNNNRTG 1 cut(s) 1295
SmlI CTYRAG 1 cut(s) 1018
SmoI CTYRAG 1 cut(s) 1018
SsiI CCGC 6 cut(s) 14, 461, 524, 1304, 1707, 1901
SspI AATATT 1 cut(s) 637
SspMI CTAG 7 cut(s) 26, 1055, 1148, 1472, 1493, 2087, 2479
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 4 cut(s) 116, 559, 1245, 1497
StyI CCWWGG 1 cut(s) 2368
TaiI ACGT 1 cut(s) 403
TaqI TCGA 8 cut(s) 123, 539, 810, 1366, 1623, 1911, 2392, 2431
TatI WGTACW 6 cut(s) 22, 279, 440, 745, 1714, 2131
TfiI GAWTC 8 cut(s) 314, 833, 1118, 1136, 1151, 1809, 2047, 2425
TscAI CASTG 6 cut(s) 51, 144, 559, 655, 1071, 1336
TseFI GTSAC 3 cut(s) 401, 1444, 1574
TseI GCWGC 4 cut(s) 1191, 2037, 2308, 2475
Tsp45I GTSAC 3 cut(s) 401, 1444, 1574
TspGWI ACGGA 2 cut(s) 1059, 1181
TspRI CASTG 6 cut(s) 51, 144, 559, 655, 1071, 1336
Van91I CCANNNNNTGG 3 cut(s) 172, 911, 1557
VpaK11BI GGWCC 4 cut(s) 491, 805, 822, 1243
VspI ATTAAT 1 cut(s) 1766
XapI RAATTY 7 cut(s) 79, 899, 907, 1771, 1948, 2021, 2113
XbaI TCTAGA 1 cut(s) 1147
XceI RCATGY 1 cut(s) 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 1369
XmnI GAANNNNTTC 6 cut(s) 35, 318, 516, 837, 1173, 2388
XspI CTAG 7 cut(s) 26, 1055, 1148, 1472, 1493, 2087, 2479
ZrmI AGTACT 1 cut(s) 2133
Zsp2I ATGCAT 1 cut(s) 1942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.