MD02G1164300.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000039
Physical Location & Seq
Forward (+)
14027404 .. 14031542
4139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1164300.v1.1.491

Sequence Viewer

Length: 2439 bp
ATGGCAGAAGTTGGGGGGCTTTTTGCAGGGGCTGCTGTTGGAACATTGTTTACTCTGCTGTATGATGTTGTTAAGGATGTCAAGGACAAGACTATGATGTTTAAGGAGCTCCTCGGAGATCTCAATTCCACGCTAGAAGCGTTAAAGCCGCTGATCTATGACATAGCGAAGTACAATGCGGTGTTGGATCAATCAAACAAGGAAGTAGAAAAAATTCTGAAACAAATGAAGAATGGGAAAGAGTTAGTTCTCGAGTGCTCCAAGCCAAGCCGGTGGAAACTCTTCAAAAAGTATAAATACACCGAGCGACTTCTCAATTTGGACAAGTGTCTGAAAAGGTTGTTGATGATACTGGCGGTGGAGGGAACAAGGAATACGTTTGAGAACTTGGCTGTTTCAAGGGAGATAGTTGGTGTTGTGAGGGAGGACTTGGCTGTTGCAAGGGTGACATTTGGTGTTGCGAGGGAGAACTTGGCTGTTTCAAGGGAGTCATTTGGTGTTGCGAGGGCGAACTTGGCTGTTTCAGGGGAGACATTTGTTCCAGAACCTCCACATGTTACAGTTGGATTGGATGAGCCTTTGAGGTATATGAAGAAGATGCTTCTGAAGGGCGATGTCTCAATGTTAGTGCTGACTGCTACGGGAGGATGCGGGAAAACCACATTGGCAATGAAATTCTGTCAAGATGAGGACGTCAAAGGTAAATTCAAGGAAAATATATTCTTTGTCACTGTTTCGGAACACCCCAACTCAGATGTAGAAAAGCTATATCAACGCATAAAGTCTCAGGAAGCGGGACAAGATCTACTGTTGGTCCTGGATGATGTTTGGTCCAACACTGTTCTTCAGAAGTTTGTTGAATTGAAGTTGCAAAATTACAAGATCTTGGTGACATCGAGATCTGAATTTCCAGGATTTGGCACTCCGTATAAACTCAAACCTTTGGAGTACAAAGATTCAATGACTCTTTTGCGTCATTCAGCAGCCCTGGGAGATAGCTTGTCTGTTCCAGAAGATCTCCTGAGAGAGATACTAAAGCACTGTAAGGGAGTTCCGCTTGCCATTACAGTGACCGGGAACTCACTTAGTGGACAAGCAGCAGAATCCTGGCTTTCAAGACTAGAAGACTGGCGTAGAGGTTCTATTCTTGATTATGAGACTAAGTTGCTTCTTTGCCTTAAAAGTAGCATAGATGCCTTAGGTGACAAAGAGCCTATCATCAGGGAATGTTTCATGGACCTTGTTTCATTTCCTGAAGATCAAAGAATCTCTGCTGCTGCTTTCATTGATATGTGGTCAGAGTTATATATGCTAGATGGGGACTTTCGGTCCATCAAGAACCTCCACGAGCTCGCCAGGAAAGGTCTGGCAAATCTTGTGATCACTAGCAGGAATGAGAATATGGCGATTCTGGATAACTACTATAGCGAACACTTTGTTCGCCAGCATGATATGCTTAGAGATCTGGGTATCTACATTGCGAAACTAGACCCAATAGAAGATAGAAAAAGACTGATTGTCGTAAGTGGACGCAATCAACCCAAGTGGTTGAGGGAGCAGAAGTCTCAACCCATCAATGCTCGCCTATTATCTATCTCCTCTGATGGAACGTACCCAACACAATGGCAGAGTGTTCATCTACCACAAGTTGAGGTTCTAGTTTTGAATTTTAAGACAGAGAACAATGCCTTACCTGAATTTGTGCAGAAAATTGACAAACTGAAGGTTCTAATAGTCACAAACTATGGTTTATCACCTACTGAATTGAGTAATTTTAATCTTCTGGAATCATTATCAAATCTGAAGAGAATCAGATTAGAGAGCATTTCAATTCATTCCATAACCAAGAACTGGGTACCATTGAAGAGTCTAAAGAAGATATCGTTATTCATGTGTAATATGGGTCAAGCTTTCCACAATTGTTCCATCAAAATTTCTGATGCCTGGCCAGGTCTAGAGGAAATGAATATCGACTATTGCGATGATTTGGTGGAACTGCCTGCCGAGCTCTGTGATCTTGTTCATATGAAGGTGCTTAGTATCACCAACTGCCATCAGCTATCTTGCTTGCCTGAAGAGATCGGAAATCTGAAGAATTTGGATGTATTGAGACTAAGGTCCTGTACAGAGTTGGTAAGGCTTCCGGTTTCAATTGAAAACCTCACTAAGTTGCGCTGCCTTGACATGTATAATTGTTTCGGCATTAGGAAGTTGCCTGAAGGCATTGGTAAGATGAGAGGTTTAAGAAAGATCAACATGGGACAATGCTCAAGATTGGAGGAGTTGCCTCTATCAGTGTGGGATCTTGACGAGCAGTTAGAGGAAGTGATTTGTGATGAAGAGAAGAAACCTTTCTGGGATTCCTTCTTAGCCAGAGACAAAATCAAGGTGACAAAAGAAAAATCCAATCTGAATTGGCTCGAAAAGCCTCAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

813

Amino Acids

92.43

Weight (kDa)

6.88

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 130 1.3e-26 Arabidopsis broad-spectrum mildew resistance protein RPW8
LRR_14 PF23598 668 - 756 2.8e-15 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 696
Acc65I GGTACC 1 cut(s) 1855
AccB1I GGYRCC 1 cut(s) 1855
AccB7I CCANNNNNTGG 2 cut(s) 917, 1851
AciI CCGC 6 cut(s) 149, 179, 356, 651, 794, 1055
AclWI GGATC 2 cut(s) 195, 2310
AcoI YGGCCR 1 cut(s) 1946
AcsI RAATTY 8 cut(s) 213, 674, 704, 905, 1666, 1697, 1932, 2095
AcuI CTGAAG 8 cut(s) 626, 830, 1275, 1742, 1823, 2094, 2111, 2238
AcyI GRCGYC 1 cut(s) 693
AdeI CACNNNGTG 1 cut(s) 1088
AfaI GTAC 5 cut(s) 173, 950, 1613, 1857, 2125
AfiI CCNNNNNNNGG 2 cut(s) 917, 1851
AflIII ACRYGT 2 cut(s) 553, 2184
AhdI GACNNNNNGTC 2 cut(s) 1327, 1517
AjnI CCWGG 7 cut(s) 816, 910, 987, 1106, 1355, 1943, 1948
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AluBI AGCT 7 cut(s) 109, 766, 999, 1351, 1910, 2008, 2059
AluI AGCT 7 cut(s) 109, 766, 999, 1351, 1910, 2008, 2059
Alw21I GWGCWC 4 cut(s) 111, 260, 1353, 2010
Alw26I GTCTC 7 cut(s) 524, 622, 789, 1151, 1569, 2104, 2370
AlwI GGATC 2 cut(s) 195, 2310
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 1 cut(s) 1946
ApeKI GCWGC 6 cut(s) 32, 983, 1097, 1274, 1277, 2175
ApoI RAATTY 8 cut(s) 213, 674, 704, 905, 1666, 1697, 1932, 2095
Asp700I GAANNNNTTC 3 cut(s) 213, 281, 2351
Asp718I GGTACC 1 cut(s) 1855
AspLEI GCGC 1 cut(s) 2175
AspS9I GGNCC 5 cut(s) 814, 831, 1237, 1329, 2118
AsuC2I CCSGG 1 cut(s) 1075
AsuHPI GGTGA 6 cut(s) 457, 901, 1214, 1746, 2035, 2401
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 5 cut(s) 814, 831, 1237, 1329, 2118
AxyI CCTNAGG 1 cut(s) 1198
BalI TGGCCA 1 cut(s) 1948
BanI GGYRCC 1 cut(s) 1855
BanII GRGCYC 3 cut(s) 111, 1353, 2010
BauI CACGAG 1 cut(s) 1346
BbsI GAAGAC 1 cut(s) 1131
Bbv12I GWGCWC 4 cut(s) 111, 260, 1353, 2010
BbvI GCAGC 6 cut(s) 19, 995, 1109, 1261, 1264, 2162
BccI CCATC 6 cut(s) 1310, 1340, 1580, 1598, 1934, 2061
BciT130I CCWGG 7 cut(s) 818, 912, 989, 1108, 1357, 1945, 1950
BclI TGATCA 1 cut(s) 1380
BcnI CCSGG 1 cut(s) 1075
BcoDI GTCTC 7 cut(s) 524, 622, 789, 1151, 1569, 2104, 2370
BfaI CTAG 7 cut(s) 134, 1121, 1313, 1386, 1487, 1658, 1955
BfmI CTRYAG 1 cut(s) 1423
BglII AGATCT 6 cut(s) 118, 802, 882, 899, 1015, 1462
BisI GCNGC 7 cut(s) 33, 149, 984, 1098, 1275, 1278, 2176
BlsI GCNGC 7 cut(s) 34, 150, 985, 1099, 1276, 1279, 2177
Bme1390I CCNGG 8 cut(s) 818, 912, 989, 1075, 1108, 1357, 1945, 1950
Bme18I GGWCC 5 cut(s) 814, 831, 1237, 1329, 2118
BmeRI GACNNNNNGTC 2 cut(s) 1327, 1517
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 5 cut(s) 814, 831, 1237, 1329, 2118
BmiI GGNNCC 1 cut(s) 1857
BmrFI CCNGG 8 cut(s) 818, 912, 989, 1075, 1108, 1357, 1945, 1950
BmrI ACTGGG 1 cut(s) 1861
BmsI GCATC 4 cut(s) 588, 638, 1183, 1930
BmuI ACTGGG 1 cut(s) 1861
BoxI GACNNNNGTC 2 cut(s) 327, 2116
BpiI GAAGAC 1 cut(s) 1131
BpuEI CTTGAG 1 cut(s) 2254
BpuMI CCSGG 1 cut(s) 1075
BsaHI GRCGYC 1 cut(s) 693
BsaJI CCNNGG 3 cut(s) 112, 987, 988
BsaWI WCCGGW 1 cut(s) 2143
BsaXI ACNNNNNCTCC 2 cut(s) 1547, 1577
Bsc4I CCNNNNNNNGG 2 cut(s) 917, 1851
Bse118I RCCGGY 1 cut(s) 270
Bse1I ACTGG 3 cut(s) 357, 1133, 1856
Bse21I CCTNAGG 1 cut(s) 1198
Bse3DI GCAATG 2 cut(s) 675, 1476
BseBI CCWGG 7 cut(s) 818, 912, 989, 1108, 1357, 1945, 1950
BseDI CCNNGG 3 cut(s) 112, 987, 988
BseGI GGATG 5 cut(s) 82, 577, 653, 826, 2107
BseLI CCNNNNNNNGG 2 cut(s) 917, 1851
BseMI GCAATG 2 cut(s) 675, 1476
BseMII CTCAG 3 cut(s) 765, 800, 1013
BseNI ACTGG 3 cut(s) 357, 1133, 1856
BseRI GAGGAG 3 cut(s) 101, 1588, 2294
BseXI GCAGC 6 cut(s) 19, 995, 1109, 1261, 1264, 2162
BsgI GTGCAG 1 cut(s) 1724
BshFI GGCC 1 cut(s) 1948
BshNI GGYRCC 1 cut(s) 1855
BsiHKAI GWGCWC 4 cut(s) 111, 260, 1353, 2010
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 3 cut(s) 271, 1074, 2144
BslFI GGGAC 3 cut(s) 810, 1334, 2274
BslI CCNNNNNNNGG 2 cut(s) 917, 1851
BsmAI GTCTC 7 cut(s) 524, 622, 789, 1151, 1569, 2104, 2370
BsmFI GGGAC 3 cut(s) 810, 1334, 2274
BsnI GGCC 1 cut(s) 1948
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 4 cut(s) 111, 260, 1353, 2010
Bsp1407I TGTACA 1 cut(s) 2123
BspACI CCGC 6 cut(s) 149, 179, 356, 651, 794, 1055
BspANI GGCC 1 cut(s) 1948
BspCNI CTCAG 3 cut(s) 764, 799, 1014
BspLI GGNNCC 1 cut(s) 1857
BspPI GGATC 2 cut(s) 195, 2310
BspT107I GGYRCC 1 cut(s) 1855
BsrDI GCAATG 2 cut(s) 675, 1476
BsrFI RCCGGY 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 2123
BsrI ACTGG 3 cut(s) 357, 1133, 1856
BssAI RCCGGY 1 cut(s) 270
BssECI CCNNGG 3 cut(s) 112, 987, 988
BssNI GRCGYC 1 cut(s) 693
BssSI CACGAG 1 cut(s) 1346
Bst2BI CACGAG 1 cut(s) 1346
Bst2UI CCWGG 7 cut(s) 818, 912, 989, 1108, 1357, 1945, 1950
Bst4CI ACNGT 6 cut(s) 562, 733, 810, 841, 1043, 1069
Bst6I CTCTTC 5 cut(s) 287, 1799, 1859, 2070, 2334
BstACI GRCGYC 1 cut(s) 693
BstAPI GCANNNNNTGC 2 cut(s) 32, 1453
BstAUI TGTACA 1 cut(s) 2123
BstC8I GCNNGC 6 cut(s) 1059, 1353, 1445, 1582, 2001, 2069
BstF5I GGATG 5 cut(s) 82, 577, 653, 826, 2107
BstHHI GCGC 1 cut(s) 2175
BstMAI GTCTC 7 cut(s) 524, 622, 789, 1151, 1569, 2104, 2370
BstMWI GCNNNNNNNGC 5 cut(s) 32, 515, 1453, 2005, 2425
BstNI CCWGG 7 cut(s) 818, 912, 989, 1108, 1357, 1945, 1950
BstNSI RCATGY 2 cut(s) 557, 2188
BstPAI GACNNNNGTC 2 cut(s) 327, 2116
BstSCI CCNGG 8 cut(s) 816, 910, 987, 1073, 1106, 1355, 1943, 1948
BstSFI CTRYAG 1 cut(s) 1423
BstV1I GCAGC 6 cut(s) 19, 995, 1109, 1261, 1264, 2162
BstV2I GAAGAC 1 cut(s) 1131
BstX2I RGATCY 7 cut(s) 118, 802, 882, 899, 1015, 1462, 2302
BstXI CCANNNNNNTGG 2 cut(s) 273, 1623
BstYI RGATCY 7 cut(s) 118, 802, 882, 899, 1015, 1462, 2302
Bsu36I CCTNAGG 1 cut(s) 1198
BsuRI GGCC 1 cut(s) 1948
BtgZI GCGATG 2 cut(s) 627, 1995
BtsCI GGATG 5 cut(s) 82, 577, 653, 826, 2107
BtsIMutI CAGTG 5 cut(s) 729, 837, 1039, 1074, 2301
Cac8I GCNNGC 6 cut(s) 1059, 1353, 1445, 1582, 2001, 2069
CaiI CAGNNNCTG 1 cut(s) 32
CfoI GCGC 1 cut(s) 2175
Cfr10I RCCGGY 1 cut(s) 270
Cfr13I GGNCC 5 cut(s) 814, 831, 1237, 1329, 2118
CseI GACGC 2 cut(s) 962, 1539
Csp6I GTAC 5 cut(s) 172, 949, 1612, 1856, 2124
CspCI CAANNNNNGTGG 2 cut(s) 1526, 1561
CviAII CATG 6 cut(s) 554, 1234, 1448, 1891, 2185, 2257
CviQI GTAC 5 cut(s) 172, 949, 1612, 1856, 2124
DraIII CACNNNGTG 1 cut(s) 1088
DriI GACNNNNNGTC 2 cut(s) 1327, 1517
EaeI YGGCCR 1 cut(s) 1946
Eam1104I CTCTTC 5 cut(s) 287, 1799, 1859, 2070, 2334
Eam1105I GACNNNNNGTC 2 cut(s) 1327, 1517
EarI CTCTTC 5 cut(s) 287, 1799, 1859, 2070, 2334
Ecl136II GAGCTC 3 cut(s) 109, 1351, 2008
Eco24I GRGCYC 3 cut(s) 111, 1353, 2010
Eco32I GATATC 1 cut(s) 1881
Eco47I GGWCC 5 cut(s) 814, 831, 1237, 1329, 2118
Eco53kI GAGCTC 3 cut(s) 109, 1351, 2008
Eco57I CTGAAG 8 cut(s) 626, 830, 1275, 1742, 1823, 2094, 2111, 2238
Eco81I CCTNAGG 1 cut(s) 1198
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 3 cut(s) 109, 1351, 2008
EcoO109I RGGNCCY 1 cut(s) 2118
EcoRII CCWGG 7 cut(s) 816, 910, 987, 1106, 1355, 1943, 1948
EcoRV GATATC 1 cut(s) 1881
EcoT38I GRGCYC 3 cut(s) 111, 1353, 2010
FaeI CATG 6 cut(s) 557, 1237, 1451, 1894, 2188, 2260
FaqI GGGAC 3 cut(s) 810, 1334, 2274
FatI CATG 6 cut(s) 553, 1233, 1447, 1890, 2184, 2256
FauI CCCGC 2 cut(s) 644, 787
FauNDI CATATG 1 cut(s) 2025
FbaI TGATCA 1 cut(s) 1380
Fnu4HI GCNGC 7 cut(s) 33, 149, 984, 1098, 1275, 1278, 2176
FokI GGATG 5 cut(s) 89, 584, 660, 833, 2114
FriOI GRGCYC 3 cut(s) 111, 1353, 2010
Fsp4HI GCNGC 7 cut(s) 33, 149, 984, 1098, 1275, 1278, 2176
FspBI CTAG 7 cut(s) 134, 1121, 1313, 1386, 1487, 1658, 1955
GlaI GCGC 1 cut(s) 2174
GluI GCNGC 7 cut(s) 33, 149, 984, 1098, 1275, 1278, 2176
HaeIII GGCC 1 cut(s) 1948
HapII CCGG 3 cut(s) 271, 1074, 2144
HgaI GACGC 2 cut(s) 962, 1539
HhaI GCGC 1 cut(s) 2175
Hin1I GRCGYC 1 cut(s) 693
Hin1II CATG 6 cut(s) 557, 1237, 1451, 1894, 2188, 2260
Hin6I GCGC 1 cut(s) 2173
HinP1I GCGC 1 cut(s) 2173
HindIII AAGCTT 1 cut(s) 1908
HpaII CCGG 3 cut(s) 271, 1074, 2144
HphI GGTGA 6 cut(s) 457, 901, 1214, 1746, 2035, 2401
Hpy166II GTNNAC 3 cut(s) 51, 1091, 1529
Hpy8I GTNNAC 3 cut(s) 51, 1091, 1529
HpyAV CCTTC 5 cut(s) 601, 1717, 2023, 2213, 2374
HpyCH4III ACNGT 6 cut(s) 562, 733, 810, 841, 1043, 1069
HpyCH4IV ACGT 3 cut(s) 377, 693, 1610
HpyCH4V TGCA 4 cut(s) 26, 440, 871, 1705
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 515, 1453, 2005, 2425
HpySE526I ACGT 3 cut(s) 377, 693, 1610
Hsp92I GRCGYC 1 cut(s) 693
Hsp92II CATG 6 cut(s) 557, 1237, 1451, 1894, 2188, 2260
HspAI GCGC 1 cut(s) 2173
KpnI GGTACC 1 cut(s) 1859
Ksp22I TGATCA 1 cut(s) 1380
LmnI GCTCC 4 cut(s) 106, 114, 263, 1555
Lsp1109I GCAGC 6 cut(s) 19, 995, 1109, 1261, 1264, 2162
LweI GCATC 4 cut(s) 588, 638, 1183, 1930
MaeI CTAG 7 cut(s) 134, 1121, 1313, 1386, 1487, 1658, 1955
MaeII ACGT 3 cut(s) 377, 693, 1610
MaeIII GTNAC 8 cut(s) 445, 556, 727, 889, 1069, 1202, 1735, 2389
MfeI CAATTG 2 cut(s) 1918, 2151
MflI RGATCY 7 cut(s) 118, 802, 882, 899, 1015, 1462, 2302
MhlI GDGCHC 4 cut(s) 111, 260, 1353, 2010
MlsI TGGCCA 1 cut(s) 1948
MluNI TGGCCA 1 cut(s) 1948
MlyI GAGTC 3 cut(s) 497, 958, 1876
MmeI TCCRAC 4 cut(s) 19, 165, 544, 858
Mox20I TGGCCA 1 cut(s) 1948
MroXI GAANNNNTTC 3 cut(s) 213, 281, 2351
MscI TGGCCA 1 cut(s) 1948
MseI TTAA 7 cut(s) 72, 102, 143, 1179, 1671, 1776, 2243
MslI CAYNNNNRTG 2 cut(s) 1067, 1289
Msp20I TGGCCA 1 cut(s) 1948
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 3 cut(s) 271, 1074, 2144
MspR9I CCNGG 8 cut(s) 818, 912, 989, 1075, 1108, 1357, 1945, 1950
MunI CAATTG 2 cut(s) 1918, 2151
MvaI CCWGG 7 cut(s) 818, 912, 989, 1108, 1357, 1945, 1950
MwoI GCNNNNNNNGC 5 cut(s) 32, 515, 1453, 2005, 2425
NciI CCSGG 1 cut(s) 1075
NdeI CATATG 1 cut(s) 2025
NlaIII CATG 6 cut(s) 557, 1237, 1451, 1894, 2188, 2260
NlaIV GGNNCC 1 cut(s) 1857
NmeAIII GCCGAG 1 cut(s) 2029
NmuCI GTSAC 7 cut(s) 445, 727, 889, 1069, 1202, 1735, 2389
NspI RCATGY 2 cut(s) 557, 2188
PaeR7I CTCGAG 1 cut(s) 251
PasI CCCWGGG 1 cut(s) 988
PciI ACATGT 2 cut(s) 553, 2184
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 1977
PdmI GAANNNNTTC 3 cut(s) 213, 281, 2351
PfeI GAWTC 7 cut(s) 956, 1103, 1266, 1408, 1787, 1809, 2360
PflMI CCANNNNNTGG 2 cut(s) 917, 1851
PfoI TCCNGGA 2 cut(s) 816, 910
PkrI GCNGC 7 cut(s) 34, 150, 985, 1099, 1276, 1279, 2177
PleI GAGTC 3 cut(s) 496, 958, 1875
PpsI GAGTC 3 cut(s) 496, 958, 1875
PpuMI RGGWCCY 1 cut(s) 2118
PscI ACATGT 2 cut(s) 553, 2184
PshAI GACNNNNGTC 2 cut(s) 327, 2116
Psp124BI GAGCTC 3 cut(s) 111, 1353, 2010
Psp5II RGGWCCY 1 cut(s) 2118
Psp6I CCWGG 7 cut(s) 816, 910, 987, 1106, 1355, 1943, 1948
PspGI CCWGG 7 cut(s) 816, 910, 987, 1106, 1355, 1943, 1948
PspN4I GGNNCC 1 cut(s) 1857
PspPI GGNCC 5 cut(s) 814, 831, 1237, 1329, 2118
PspPPI RGGWCCY 1 cut(s) 2118
PsrI GAACNNNNNNTAC 4 cut(s) 34, 66, 358, 390
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 7 cut(s) 118, 802, 882, 899, 1015, 1462, 2302
RsaI GTAC 5 cut(s) 173, 950, 1613, 1857, 2125
RsaNI GTAC 5 cut(s) 172, 949, 1612, 1856, 2124
RseI CAYNNNNRTG 2 cut(s) 1067, 1289
SacI GAGCTC 3 cut(s) 111, 1353, 2010
SaqAI TTAA 7 cut(s) 72, 102, 143, 1179, 1671, 1776, 2243
SatI GCNGC 7 cut(s) 33, 149, 984, 1098, 1275, 1278, 2176
Sau96I GGNCC 5 cut(s) 814, 831, 1237, 1329, 2118
SchI GAGTC 3 cut(s) 497, 958, 1876
ScrFI CCNGG 8 cut(s) 818, 912, 989, 1075, 1108, 1357, 1945, 1950
SduI GDGCHC 4 cut(s) 111, 260, 1353, 2010
SfaNI GCATC 4 cut(s) 588, 638, 1183, 1930
SfcI CTRYAG 1 cut(s) 1423
Sfr274I CTCGAG 1 cut(s) 251
SinI GGWCC 5 cut(s) 814, 831, 1237, 1329, 2118
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 2 cut(s) 1067, 1289
SmlI CTYRAG 2 cut(s) 251, 2269
SmoI CTYRAG 2 cut(s) 251, 2269
SsiI CCGC 6 cut(s) 149, 179, 356, 651, 794, 1055
SspMI CTAG 7 cut(s) 134, 1121, 1313, 1386, 1487, 1658, 1955
SstI GAGCTC 3 cut(s) 111, 1353, 2010
StyD4I CCNGG 8 cut(s) 816, 910, 987, 1073, 1106, 1355, 1943, 1948
TaaI ACNGT 6 cut(s) 562, 733, 810, 841, 1043, 1069
TaiI ACGT 3 cut(s) 380, 696, 1613
TaqI TCGA 4 cut(s) 252, 896, 1971, 2421
TaqII GACCGA 1 cut(s) 1317
TatI WGTACW 3 cut(s) 171, 948, 2123
TauI GCSGC 1 cut(s) 151
TfiI GAWTC 7 cut(s) 956, 1103, 1266, 1408, 1787, 1809, 2360
Tru1I TTAA 7 cut(s) 72, 102, 143, 1179, 1671, 1776, 2243
Tru9I TTAA 7 cut(s) 72, 102, 143, 1179, 1671, 1776, 2243
TscAI CASTG 5 cut(s) 736, 844, 1046, 1074, 2301
TseFI GTSAC 7 cut(s) 445, 727, 889, 1069, 1202, 1735, 2389
TseI GCWGC 6 cut(s) 32, 983, 1097, 1274, 1277, 2175
Tsp45I GTSAC 7 cut(s) 445, 727, 889, 1069, 1202, 1735, 2389
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 5 cut(s) 736, 844, 1046, 1074, 2301
Van91I CCANNNNNTGG 2 cut(s) 917, 1851
VpaK11BI GGWCC 5 cut(s) 814, 831, 1237, 1329, 2118
XapI RAATTY 8 cut(s) 213, 674, 704, 905, 1666, 1697, 1932, 2095
XbaI TCTAGA 1 cut(s) 1954
XceI RCATGY 2 cut(s) 557, 2188
XcmI CCANNNNNNNNNTGG 1 cut(s) 1363
XhoI CTCGAG 1 cut(s) 251
XmnI GAANNNNTTC 3 cut(s) 213, 281, 2351
XspI CTAG 7 cut(s) 134, 1121, 1313, 1386, 1487, 1658, 1955
ZraI GACGTC 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.