RchiOBHm_Chr2g0104611

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000039
Physical Location & Seq
Forward (+)
15934580 .. 15942444
7865 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47887

Sequence Viewer

Length: 5070 bp
ATGACAGTGGCATCATTAGAAGGACGAGTGGAAGCAGCATTTCTTGCCCTGTTTGGTATTGTTTTGGAAGTGAAGGACAAGAATGTGATGTATAGACCACTTATGGGATCTATCAAATCCAGTCTGGACTGCTTAAAGCCATTGATCGAAGACATGGCACAAGGAAACAGGGTCCTGGATCGTCCAGTTAAGGAACTAGAGTATTTCAGAATGCATATGGAGAAAGGTGCAGAGCTAGTCCGCAAGTGCTCCAAAAATGGTGTGTGCGCCAAATACAAAAAGAAGAAATACACCAACCAACTTCTTGAGTTGGATGCATGTCTTCAGAGGCAGTTCAATGTACTGAGAGCGCAGGTTGCAAGAGATGTGAGGGAGACCACAGCTTCGATAAAGAGTATACAGAAGGTGATCAAGCGAATTGAGGAGAGTGGTGTCATGAAGAATCAAATGGAAATGAATGGTCCGTGTGAAGTAGTTGAGGCTTCATCCCCTGATGAACAGAATGAGGGTTTGGTTGAACCTTCATCCCCTGATGAACAGAATAAGGGAGTTGGAAAAATGGAGGCATCAGATATAAGAGTTGAAGGCAGTGGTGAGGTCCAAGAAAAGAGTGAAGTTCCAAATTGTTTTGAAGTACCTGAACCTCCATTGTTTACAGTTGGGTTGGATGTGGCTTTGAAAGAGATGAAGATGAAGCTCCTGGAGGATGGGTTTACGAAGATTGTTGTCTGGGGTGTTGGAGGAGTCGGGAAGACCACATTGGTGAAAATGTTGTGTCATGATCAGGAAGTCAAAGATAAATTCAAGAAAATCTTCTTTACCACTGCTTCAAAATGGCAGAACCTGAACCTCATTATGCAAGAACTATATCGAGTCCTGTGTTCCCAGATTCCTACTTTCCAAAACAAAGAAGGGAGGCGTTATCTGGTTGTCCTGGATGATGTTGGGGATGGATCAGAATCCATTCTTCCGGAGTTTGATGGATTGAAAATTCCAAATAGCAAGATTTTGATTACATCAAGATATCCATTTCCAGGAGTTGGTTCTCAATACCATTTGAAATCTCTGAATGATGAGGATGCAATGTCTCTTTTTCATCACTCAGCATCATTGGGAGATCAGTGCTCTTATATTCCAGAAGATCTTTTGAGAAAGATAGTAGAGTGTTGTAAGGGACTTCCACTTGCAATCACTGTGAACGGAAGATCACTTTGCGGGCAACCTATTGAAATCTGGCAAAAGAGAATAGTAGAATGGGGGAGAGGTTCTTCTATTTTTGATACAGAACCAGAAATGATTGTTTGCCTCCAAAGAAGCTTGGATGCCTTACATAGAGAAAAGACTATCATCAAAGAATGTTTTGTAGACCTTGCTTCATTTCCTGAAGACCAAGTAATCCCTGCTGCTGCCCTCATTGATATGTGGGCAGAATTATATGGACTAGATGAAGAGTATTGGTCCATTGCAAACCTCCAGAAGCTCACCATCCGCGGATTTGCCAATATTGTCTCAAGGGAGGAGGAGATGGAGGTGGATGGCTATTACAGTGATCTCTTTGTTACCCTGCATCATATGCTTAGAGGGCTGGCAATCCACAAGGCTAGTGAGGACACAATAGAACAGAGAAATAGACTGATTATAGACATACATGGAGACAATCTTCCTAGCTGGTGGACAGAACAGAAGTATCAGCCTAGGAATGCTCGTCTACTATCTGTCTCAACTGATCGAGCGTTCTCAAGAAAATGGCACTACATGCAAGTACCAAAAGTTGAGGTTCTAGTTTTGAACTTCCAGACTGAGAAATATGAGTTACCTGATTTTCTGGATAAAATAGACAAATTAAAGGTTCTAGTAGTCACAAACTATGGCTCCTCGTCTGCTCAGTTAAGTAACTTTCAACTGGTAGGCTCATTGCGAAATCTGAAAAGAATCAGATTAGAGGGAGTTTCGATTACTTCCATAACCAAGAACCCCATACAACTGGATAGTCTAAAGAAGATAGCTTTTACCAAGTGTGACATGGGTCAAGCTTTTGGCAACTCTTCCTTTCAATTTTCTATAGCATTTCCAAATCTAGTGGAGATGTATGTCGAGCATTGCCACGATTTGAAGGAATTGCCTGCTGACCTTGGTAATCTTTCCCACCTAGAGACACTCTCTATCTCCAATTGCCATAGGCTGTCCACCTTGCCTGAAGGGATTGGAAATCTGAGCAAGTTGAAAAGGTTGAGGGTTAGGTATGGTACAAACTTTGTAAAGTTTCCAGGATCAATTAGGAAGCTCAATAGCTTAGAGTTTCTTGACATATCTGGTTGCATCAAAATCATGGGTTTCCCTGAAGACATTGGTGAAATGAGCAGATTAAGAAAGCTGAACATAAGTGGTTGCTCAAGATTGAAAGAGCTGCCACTGTCTGTTTTGAGTCTTGAGCAGTTACAGGAAGTGATAATGGATGAAGAGACAGAAAAGTCATGGAATCCTTTCCTACTCAAAAACATAGACACAAGGGTGGTTCCTCCACTTACAGTTGGATTTGATGTGGCTTTGGAGGAGTTGAACTGTCAGGTACTCAAGGATAGGATGTCCAGACGTGTCGAGTGTGGTATAGCACAAAATAAAATTCAAACCGAAGGTTGGTGTCCTGTACCTGTGCCTTCTTCGCTCACTATGGGATTAGATGTCCTCAACGTGTGGGGAGCAATGAAGATGGATGAGAAATTGACTTCAGAAGTGAGGGTCAAGCAAATTGAAGGGAGTGATGTGGTGAAAAAACAACTTGGAATTAAAGGGATATCCGGGAATCACAGCAGGAACAACATAGTTTCCAATGCGAGCAGCAGTGCAGGAGGAAGTCAATTGTTGAAGGCAGCAGAGACAGATGAGTCGCAGACTGATGAGCAAAGTTTGGAGACATTTGTCTTAAGACGTTTCAATTTTGAAGAGTTGAATGTTGCTACTGGGAATTTCAGGTCAGATGGAATAATTGGTAGGGGGCCCTTTGGTAACATTTTCAAGGGTTGGGTGGATGAGAAGACGCTTGCACCTTCTAAGGTTGGCTTTGGAATGCCTGTTGCTGTCAAGAAACTGAATCCAGAAAGAGTGCTAGATTTTGAAGAGTGGCAGTCAGAGTTGAACTTTCGAGGAAGGACTTCTCACCCCAACCTTGTAAAGCTATTGGGTTACTGTCGTGAGGAAAAGGAGATGCTCTTTGTTTATGATTTCATGCCTAATGGAAGCTTGGAAGATCATTTTCATAGAAGGAGCCCTGGCATTGAAACACTACCTTGGAACTACAGACTCGATGCAGCTTTTGGCATGGAACCCCTATCTTGGAACAAAAGACTGGATATAGCTTCTGGAGTAGCTCAAGCCCTAGCCTACTTGCATACTTTTTCACCTAATCAAATTGTCCACAGTGACCTTAAGGCCTCAAATATCTTGCTTGATAGGAATTACAATGCAAAACTCTCAAATTTTGGATTAGAAAGACTTGGGAAAACTGGTGAACTGTCACATCAATCAACTCGCTTTGTAGGAACATCTCCTTATATTGATCCAAAGGAAATTACAACAGGTCATTCTTATGTAAAGAGCGATGTTTATAGGTTTGGTGTTACACTACTTCAGTTGCTGACAGGGTTAAAGACCACTGATAGAAATCGTCCCAGCGAACGGCAAAATCTGGGGTTAAAGACCACTGATAGAAATTGTCCCAGCGAACGGCAAAATCTGGTTGAATGGGGTAAACCACTACTCTTGAATGAGAGAAATTTGAAAACCATCATGGACGTGCGGATAGAGGGCCAATATTCCTCCAAGGCAGCATTTCAAGTAGCACAACTTACTCTAAAATGCCTAGCATTGGATCCCAAAAGCCGACCTTCCACGACAGAAGTTGTGGAAGAACTGGAACAAATTCAGGCAATAGAGGAAAGAACTTTAAACACCAGTATGCCCAGCAGTGAGTGCCGGAGGAACATAGATTCGAACACTAGCAGTGGCGGAGGAACAAGTGTATTCTCGGAGGGAAAAACATCAACATTTCAAAGCAAAGATGATGATCAGTCAATTGATGGCCATATTTCAGAGACATCCAACTTGAGAGTTTTCAGTTTTGCAGAGTTGAAGACTGCGACAAGGAATTTCAGGGCAGACACAATGCTTGGTGAGGGAGGTTTTGGGATTGTATTCAAGGGCTGGTTGGATGAAACGTCGGTTTCACCTTCTAAAGCTGGCACTGGAATGATGATTGCTATCAAGAAACTGAAAGCAGAAAGTTTTCAAGGCTTTAAAGAGTGGCAGGCAGAGGTGAACTTTGTAGGAAGGCTTTCTCATCCCAACCTTGTGAAGCTGTTGGGATACTGTTGGGAAGATAAGGAGTTACTCCTTGTTTATGAGTTCATGAGCAGAGGAAGCTTGGAGAATCATCTTTTCAGACGGAATCCAAACTTTGAACCGCTATGTTGGGAAAAAAGAATCAAAATAGCTATCGGAGCAGCTCGAGGCCTTGCTTTCTTGCACAGTTTAGAAAAACAAGTTATATACAGAGATTTTAAGACCTCAAATATATTGCTTGATGGGAATTACAACGCAAAACTCTCAGATTTTGGCTTGGCGAAATTGGGGCCAACTGGTGGAGACTCGCATGTCTCAACTAGGATTATGGGAACTTACGGCTACGCAGCTCCGGAATACATTGCAACAGGTCATTTGTATGTGAAGAGTGATGTGTACAGTTTCGGTGTTGTACTACTTGAACTTCTCACAGGGTTACGAGCACTCGATACTAAACGCCCCAAGGAACAACTTAATCTGGTAAGCTGGGCTAATCGACTTCTTTCAAATAAAAGATATTTGAAAACCATCATCGATGGGCAGATGGAAGGCCAATATTCCTTCAAGGCAGCATCAAAGGCAGGACAGATTACTCTAAAATGTCTAGCACCAGATCCTAAAAACCGACCTTCCATGAAAGAAGTTGTGGAGGAACTGGAACAGATTCAGGCAATCGAGGAAAAACTTACGGCTACAGATTCAAGCAACTCCCATTTAGGTATGGTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

1689

Amino Acids

190.12

Weight (kDa)

6.64

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 11 - 138 4.1e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 226 - 367 4.2e-10 NB-ARC domain
LRR_RPS2 PF23247 682 - 816 4.8e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 703 - 794 2.1e-14 Leucine-rich repeat region
Pkinase PF00069 993 - 1302 1.5e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 995 - 1304 7.7e-36 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 1386 - 1665 6.8e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1386 - 1663 1.5e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 2470, 2884, 4649
Acc36I ACCTGC 1 cut(s) 343
AccB7I CCANNNNNTGG 2 cut(s) 1040, 4637
AccI GTMKAC 3 cut(s) 397, 1365, 1708
AccII CGCG 1 cut(s) 1491
AccIII TCCGGA 2 cut(s) 970, 4690
AciI CCGC 7 cut(s) 241, 1215, 1489, 1491, 3796, 4005, 4460
AclWI GGATC 8 cut(s) 115, 186, 961, 2278, 3551, 3863, 3876, 4946
AcoI YGGCCR 1 cut(s) 4078
AcsI RAATTY 8 cut(s) 800, 990, 2622, 2965, 3475, 3772, 3918, 4144
AcuI CTGAAG 6 cut(s) 308, 1404, 2217, 2361, 2712, 3611
AfaI GTAC 8 cut(s) 342, 636, 1764, 2248, 2571, 2649, 4736, 4752
AfiI CCNNNNNNNGG 9 cut(s) 104, 1034, 1040, 2637, 3333, 3675, 3723, 3865, 4637
AflII CTTAAG 2 cut(s) 2923, 3425
AflIII ACRYGT 2 cut(s) 2593, 2691
AjiI CACGTC 2 cut(s) 2594, 3793
AjnI CCWGG 6 cut(s) 174, 699, 933, 1033, 2266, 3268
AjuI GAANNNNNNNTTGG 4 cut(s) 494, 526, 743, 775
AleI CACNNNNGTG 2 cut(s) 761, 2510
Alw21I GWGCWC 3 cut(s) 251, 1127, 4783
AlwI GGATC 8 cut(s) 115, 186, 961, 2278, 3551, 3863, 3876, 4946
AlwNI CAGNNNCTG 2 cut(s) 844, 3634
Ama87I CYCGRG 1 cut(s) 4503
Aor13HI TCCGGA 2 cut(s) 970, 4690
AoxI GGCC 7 cut(s) 2996, 3429, 3805, 4078, 4507, 4628, 4888
ApaI GGGCCC 1 cut(s) 3000
ApoI RAATTY 8 cut(s) 800, 990, 2622, 2965, 3475, 3772, 3918, 4144
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
Asp700I GAANNNNTTC 8 cut(s) 812, 963, 2483, 3151, 3918, 4281, 4330, 5001
AspA2I CCTAGG 1 cut(s) 1694
AspLEI GCGC 2 cut(s) 269, 352
AspS9I GGNCC 8 cut(s) 172, 461, 598, 1458, 2996, 2997, 3805, 4628
AsuC2I CCSGG 1 cut(s) 2800
AsuII TTCGAA 1 cut(s) 3989
AvaI CYCGRG 1 cut(s) 4503
AvaII GGWCC 4 cut(s) 172, 461, 598, 1458
AvrII CCTAGG 1 cut(s) 1694
BaeGI GKGCMC 1 cut(s) 3000
BaeI ACNNNNGTAYC 2 cut(s) 1670, 1703
BalI TGGCCA 1 cut(s) 4080
BamHI GGATCC 1 cut(s) 3868
BanII GRGCYC 2 cut(s) 3000, 3269
BbsI GAAGAC 7 cut(s) 156, 314, 758, 1392, 2349, 3041, 4136
Bbv12I GWGCWC 3 cut(s) 251, 1127, 4783
BceAI ACGGC 4 cut(s) 3692, 3740, 4693, 5043
BciT130I CCWGG 6 cut(s) 176, 701, 935, 1035, 2268, 3270
BciVI GTATCC 1 cut(s) 4355
BclI TGATCA 3 cut(s) 408, 781, 4063
BcnI CCSGG 1 cut(s) 2800
BfmI CTRYAG 3 cut(s) 2061, 3295, 5031
BfrI CTTAAG 2 cut(s) 2923, 3425
BfuAI ACCTGC 1 cut(s) 343
BfuI GTATCC 1 cut(s) 4355
BglII AGATCT 1 cut(s) 1141
BlnI CCTAGG 1 cut(s) 1694
Bme1390I CCNGG 7 cut(s) 176, 701, 935, 1035, 2268, 2800, 3270
Bme18I GGWCC 4 cut(s) 172, 461, 598, 1458
BmeT110I CYCGRG 1 cut(s) 4503
BmgBI CACGTC 2 cut(s) 2594, 3793
BmgT120I GGNCC 8 cut(s) 172, 461, 598, 1458, 2996, 2997, 3805, 4628
BmiI GGNNCC 9 cut(s) 173, 1873, 2517, 2997, 2998, 3266, 3324, 3870, 4629
BmrFI CCNGG 7 cut(s) 176, 701, 935, 1035, 2268, 2800, 3270
BmrI ACTGGG 1 cut(s) 2970
BmuI ACTGGG 1 cut(s) 2970
BoxI GACNNNNGTC 2 cut(s) 2025, 2918
BpiI GAAGAC 7 cut(s) 156, 314, 758, 1392, 2349, 3041, 4136
BplI GAGNNNNNCTC 2 cut(s) 2144, 2176
BpmI CTGGAG 3 cut(s) 722, 1457, 3381
Bpu14I TTCGAA 1 cut(s) 3989
BpuEI CTTGAG 8 cut(s) 326, 1495, 1723, 2377, 2450, 2558, 3354, 4123
BpuMI CCSGG 1 cut(s) 2800
Bsa29I ATCGAT 1 cut(s) 4872
BsaBI GATNNNNATC 4 cut(s) 958, 3660, 4062, 4256
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 7 cut(s) 1489, 1694, 2131, 3268, 3287, 3819, 4800
BsaWI WCCGGW 2 cut(s) 970, 4690
BsaXI ACNNNNNCTCC 4 cut(s) 695, 725, 1856, 1886
Bsc4I CCNNNNNNNGG 9 cut(s) 104, 1034, 1040, 2637, 3333, 3675, 3723, 3865, 4637
Bse3DI GCAATG 6 cut(s) 1089, 1461, 1913, 2098, 2709, 4698
Bse8I GATNNNNATC 4 cut(s) 958, 3660, 4062, 4256
BseAI TCCGGA 2 cut(s) 970, 4690
BseBI CCWGG 6 cut(s) 176, 701, 935, 1035, 2268, 3270
BseCI ATCGAT 1 cut(s) 4872
BseDI CCNNGG 7 cut(s) 1489, 1694, 2131, 3268, 3287, 3819, 4800
BseJI GATNNNNATC 4 cut(s) 958, 3660, 4062, 4256
BseLI CCNNNNNNNGG 9 cut(s) 104, 1034, 1040, 2637, 3333, 3675, 3723, 3865, 4637
BseMI GCAATG 6 cut(s) 1089, 1461, 1913, 2098, 2709, 4698
BseMII CTCAG 6 cut(s) 335, 1116, 1791, 1898, 2204, 4617
BseRI GAGGAG 6 cut(s) 437, 756, 1532, 1535, 1864, 2567
BseSI GKGCMC 1 cut(s) 3000
BseYI CCCAGC 4 cut(s) 3668, 3716, 3959, 4824
BsgI GTGCAG 2 cut(s) 249, 2865
Bsh1236I CGCG 1 cut(s) 1491
BshFI GGCC 7 cut(s) 2998, 3431, 3807, 4080, 4509, 4630, 4890
BshVI ATCGAT 1 cut(s) 4872
BsiHKAI GWGCWC 3 cut(s) 251, 1127, 4783
BsiHKCI CYCGRG 1 cut(s) 4503
BsiSI CCGG 4 cut(s) 971, 2799, 3973, 4691
BslFI GGGAC 3 cut(s) 1188, 3651, 3699
BslI CCNNNNNNNGG 9 cut(s) 104, 1034, 1040, 2637, 3333, 3675, 3723, 3865, 4637
BsmFI GGGAC 3 cut(s) 1188, 3651, 3699
BsmI GAATGC 3 cut(s) 216, 1705, 3072
BsnI GGCC 7 cut(s) 2998, 3431, 3807, 4080, 4509, 4630, 4890
Bso31I GGTCTC 1 cut(s) 368
BsoBI CYCGRG 1 cut(s) 4503
Bsp119I TTCGAA 1 cut(s) 3989
Bsp120I GGGCCC 1 cut(s) 2996
Bsp1286I GDGCHC 5 cut(s) 251, 1127, 3000, 3269, 4783
Bsp13I TCCGGA 2 cut(s) 970, 4690
Bsp1407I TGTACA 1 cut(s) 4734
BspACI CCGC 7 cut(s) 241, 1215, 1489, 1491, 3796, 4005, 4460
BspANI GGCC 7 cut(s) 2998, 3431, 3807, 4080, 4509, 4630, 4890
BspCNI CTCAG 6 cut(s) 336, 1115, 1792, 1897, 2205, 4616
BspDI ATCGAT 1 cut(s) 4872
BspEI TCCGGA 2 cut(s) 970, 4690
BspFNI CGCG 1 cut(s) 1491
BspHI TCATGA 4 cut(s) 435, 778, 4404, 5066
BspLI GGNNCC 9 cut(s) 173, 1873, 2517, 2997, 2998, 3266, 3324, 3870, 4629
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 8 cut(s) 115, 186, 961, 2278, 3551, 3863, 3876, 4946
BspT104I TTCGAA 1 cut(s) 3989
BspTI CTTAAG 2 cut(s) 2923, 3425
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 6 cut(s) 1089, 1461, 1913, 2098, 2709, 4698
BsrGI TGTACA 1 cut(s) 4734
BssECI CCNNGG 7 cut(s) 1489, 1694, 2131, 3268, 3287, 3819, 4800
BssNAI GTATAC 1 cut(s) 398
BssT1I CCWWGG 5 cut(s) 1694, 2131, 3287, 3819, 4800
Bst1107I GTATAC 1 cut(s) 398
Bst2UI CCWGG 6 cut(s) 176, 701, 935, 1035, 2268, 3270
Bst6I CTCTTC 6 cut(s) 1443, 2050, 2454, 2937, 3111, 4718
BstAFI CTTAAG 2 cut(s) 2923, 3425
BstAPI GCANNNNNTGC 4 cut(s) 44, 1573, 1756, 3969
BstAUI TGTACA 1 cut(s) 4734
BstBI TTCGAA 1 cut(s) 3989
BstC8I GCNNGC 7 cut(s) 1217, 1587, 2124, 2836, 3042, 4237, 4305
BstDEI CTNAG 9 cut(s) 344, 1102, 1577, 1800, 1884, 2213, 2293, 3051, 4603
BstDSI CCRYGG 1 cut(s) 1489
BstFNI CGCG 1 cut(s) 1491
BstHHI GCGC 2 cut(s) 269, 352
BstNI CCWGG 6 cut(s) 176, 701, 935, 1035, 2268, 3270
BstNSI RCATGY 3 cut(s) 321, 1759, 4652
BstPAI GACNNNNGTC 2 cut(s) 2025, 2918
BstSCI CCNGG 7 cut(s) 174, 699, 933, 1033, 2266, 2798, 3268
BstSFI CTRYAG 3 cut(s) 2061, 3295, 5031
BstSLI GKGCMC 1 cut(s) 3000
BstUI CGCG 1 cut(s) 1491
BstV2I GAAGAC 7 cut(s) 156, 314, 758, 1392, 2349, 3041, 4136
BstX2I RGATCY 4 cut(s) 107, 1141, 3868, 4951
BstXI CCANNNNNNTGG 1 cut(s) 1984
BstYI RGATCY 4 cut(s) 107, 1141, 3868, 4951
BstZ17I GTATAC 1 cut(s) 398
Bsu15I ATCGAT 1 cut(s) 4872
BsuI GTATCC 1 cut(s) 4355
BsuRI GGCC 7 cut(s) 2998, 3431, 3807, 4080, 4509, 4630, 4890
BsuTUI ATCGAT 1 cut(s) 4872
BtgI CCRYGG 1 cut(s) 1489
BtgZI GCGATG 1 cut(s) 3612
BtrI CACGTC 2 cut(s) 2594, 3793
BtsI GCAGTG 5 cut(s) 595, 822, 2848, 3970, 4006
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 7 cut(s) 1217, 1587, 2124, 2836, 3042, 4237, 4305
CaiI CAGNNNCTG 2 cut(s) 844, 3634
CciI TCATGA 4 cut(s) 435, 778, 4404, 5066
CfoI GCGC 2 cut(s) 269, 352
Cfr13I GGNCC 8 cut(s) 172, 461, 598, 1458, 2996, 2997, 3805, 4628
Cfr42I CCGCGG 1 cut(s) 1492
ClaI ATCGAT 1 cut(s) 4872
CseI GACGC 1 cut(s) 3046
Csp6I GTAC 8 cut(s) 341, 635, 1763, 2247, 2570, 2648, 4735, 4751
CspCI CAANNNNNGTGG 4 cut(s) 2061, 2096, 3741, 3776
CviQI GTAC 8 cut(s) 341, 635, 1763, 2247, 2570, 2648, 4735, 4751
DdeI CTNAG 9 cut(s) 344, 1102, 1577, 1800, 1884, 2213, 2293, 3051, 4603
DraI TTTAAA 2 cut(s) 3945, 4294
DrdI GACNNNNNNGTC 3 cut(s) 2470, 2884, 4649
DseDI GACNNNNNNGTC 3 cut(s) 2470, 2884, 4649
EaeI YGGCCR 1 cut(s) 4078
Eam1104I CTCTTC 6 cut(s) 1443, 2050, 2454, 2937, 3111, 4718
EarI CTCTTC 6 cut(s) 1443, 2050, 2454, 2937, 3111, 4718
EciI GGCGGA 1 cut(s) 4020
Eco130I CCWWGG 5 cut(s) 1694, 2131, 3287, 3819, 4800
Eco147I AGGCCT 2 cut(s) 3431, 4509
Eco24I GRGCYC 2 cut(s) 3000, 3269
Eco31I GGTCTC 1 cut(s) 368
Eco32I GATATC 2 cut(s) 1025, 2796
Eco47I GGWCC 4 cut(s) 172, 461, 598, 1458
Eco57I CTGAAG 6 cut(s) 308, 1404, 2217, 2361, 2712, 3611
Eco88I CYCGRG 1 cut(s) 4503
EcoO109I RGGNCCY 3 cut(s) 172, 2996, 2997
EcoRII CCWGG 6 cut(s) 174, 699, 933, 1033, 2266, 3268
EcoRV GATATC 2 cut(s) 1025, 2796
EcoT14I CCWWGG 5 cut(s) 1694, 2131, 3287, 3819, 4800
EcoT22I ATGCAT 2 cut(s) 216, 319
EcoT38I GRGCYC 2 cut(s) 3000, 3269
ErhI CCWWGG 5 cut(s) 1694, 2131, 3287, 3819, 4800
FalI AAGNNNNNCTT 6 cut(s) 797, 829, 3044, 3076, 3868, 3900
FaqI GGGAC 3 cut(s) 1188, 3651, 3699
FauI CCCGC 1 cut(s) 1208
FauNDI CATATG 2 cut(s) 216, 1572
FbaI TGATCA 3 cut(s) 408, 781, 4063
FblI GTMKAC 3 cut(s) 397, 1365, 1708
FriOI GRGCYC 2 cut(s) 3000, 3269
GlaI GCGC 2 cut(s) 268, 351
GsaI CCCAGC 4 cut(s) 3672, 3720, 3963, 4828
GsuI CTGGAG 3 cut(s) 722, 1457, 3381
HaeIII GGCC 7 cut(s) 2998, 3431, 3807, 4080, 4509, 4630, 4890
HapII CCGG 4 cut(s) 971, 2799, 3973, 4691
HgaI GACGC 1 cut(s) 3046
HhaI GCGC 2 cut(s) 269, 352
Hin6I GCGC 2 cut(s) 267, 350
HinP1I GCGC 2 cut(s) 267, 350
HindIII AAGCTT 4 cut(s) 1315, 2031, 3238, 4417
HpaII CCGG 4 cut(s) 971, 2799, 3973, 4691
Hpy99I CGWCG 1 cut(s) 4219
HpyCH4IV ACGT 5 cut(s) 2593, 2691, 2929, 3792, 4214
HpyF3I CTNAG 9 cut(s) 344, 1102, 1577, 1800, 1884, 2213, 2293, 3051, 4603
HpySE526I ACGT 5 cut(s) 2593, 2691, 2929, 3792, 4214
HspAI GCGC 2 cut(s) 267, 350
Kpn2I TCCGGA 2 cut(s) 970, 4690
Ksp22I TGATCA 3 cut(s) 408, 781, 4063
KspI CCGCGG 1 cut(s) 1492
LmnI GCTCC 7 cut(s) 254, 702, 1877, 2699, 3264, 4496, 4693
MaeII ACGT 5 cut(s) 2593, 2691, 2929, 3792, 4214
MfeI CAATTG 3 cut(s) 2170, 2858, 4071
MflI RGATCY 4 cut(s) 107, 1141, 3868, 4951
MhlI GDGCHC 5 cut(s) 251, 1127, 3000, 3269, 4783
MlsI TGGCCA 1 cut(s) 4080
MluNI TGGCCA 1 cut(s) 4080
MlyI GAGTC 6 cut(s) 753, 882, 2434, 2894, 3294, 4637
MmeI TCCRAC 7 cut(s) 291, 532, 645, 718, 2512, 4122, 4185
Mox20I TGGCCA 1 cut(s) 4080
Mph1103I ATGCAT 2 cut(s) 216, 319
MroI TCCGGA 2 cut(s) 970, 4690
MroXI GAANNNNTTC 8 cut(s) 812, 963, 2483, 3151, 3918, 4281, 4330, 5001
MscI TGGCCA 1 cut(s) 4080
MslI CAYNNNNRTG 8 cut(s) 761, 1418, 2510, 3585, 3791, 3953, 4244, 4716
Msp20I TGGCCA 1 cut(s) 4080
MspA1I CMGCKG 1 cut(s) 1491
MspCI CTTAAG 2 cut(s) 2923, 3425
MspI CCGG 4 cut(s) 971, 2799, 3973, 4691
MspR9I CCNGG 7 cut(s) 176, 701, 935, 1035, 2268, 2800, 3270
MunI CAATTG 3 cut(s) 2170, 2858, 4071
Mva1269I GAATGC 3 cut(s) 216, 1705, 3072
MvaI CCWGG 6 cut(s) 176, 701, 935, 1035, 2268, 3270
MvnI CGCG 1 cut(s) 1491
NciI CCSGG 1 cut(s) 2800
NdeI CATATG 2 cut(s) 216, 1572
NlaIV GGNNCC 9 cut(s) 173, 1873, 2517, 2997, 2998, 3266, 3324, 3870, 4629
NmuCI GTSAC 4 cut(s) 1858, 2018, 3419, 3513
NsiI ATGCAT 2 cut(s) 216, 319
NspI RCATGY 3 cut(s) 321, 1759, 4652
NspV TTCGAA 1 cut(s) 3989
OliI CACNNNNGTG 2 cut(s) 761, 2510
PaeR7I CTCGAG 1 cut(s) 4503
PagI TCATGA 4 cut(s) 435, 778, 4404, 5066
PceI AGGCCT 2 cut(s) 3431, 4509
PctI GAATGC 3 cut(s) 216, 1705, 3072
PdmI GAANNNNTTC 8 cut(s) 812, 963, 2483, 3151, 3918, 4281, 4330, 5001
PflMI CCANNNNNTGG 2 cut(s) 1040, 4637
PfoI TCCNGGA 6 cut(s) 174, 699, 933, 1033, 2266, 2798
PleI GAGTC 6 cut(s) 752, 881, 2433, 2893, 3294, 4637
PpsI GAGTC 6 cut(s) 752, 881, 2433, 2893, 3294, 4637
PpuMI RGGWCCY 1 cut(s) 172
PshAI GACNNNNGTC 2 cut(s) 2025, 2918
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 6 cut(s) 174, 699, 933, 1033, 2266, 3268
PspFI CCCAGC 4 cut(s) 3668, 3716, 3959, 4824
PspGI CCWGG 6 cut(s) 174, 699, 933, 1033, 2266, 3268
PspN4I GGNNCC 9 cut(s) 173, 1873, 2517, 2997, 2998, 3266, 3324, 3870, 4629
PspOMI GGGCCC 1 cut(s) 2996
PspPI GGNCC 8 cut(s) 172, 461, 598, 1458, 2996, 2997, 3805, 4628
PspPPI RGGWCCY 1 cut(s) 172
PspXI VCTCGAGB 1 cut(s) 4503
PstNI CAGNNNCTG 2 cut(s) 844, 3634
PsuI RGATCY 4 cut(s) 107, 1141, 3868, 4951
RsaI GTAC 8 cut(s) 342, 636, 1764, 2248, 2571, 2649, 4736, 4752
RsaNI GTAC 8 cut(s) 341, 635, 1763, 2247, 2570, 2648, 4735, 4751
RseI CAYNNNNRTG 8 cut(s) 761, 1418, 2510, 3585, 3791, 3953, 4244, 4716
SacII CCGCGG 1 cut(s) 1492
Sau96I GGNCC 8 cut(s) 172, 461, 598, 1458, 2996, 2997, 3805, 4628
SchI GAGTC 6 cut(s) 753, 882, 2434, 2894, 3294, 4637
ScrFI CCNGG 7 cut(s) 176, 701, 935, 1035, 2268, 2800, 3270
SduI GDGCHC 5 cut(s) 251, 1127, 3000, 3269, 4783
SfcI CTRYAG 3 cut(s) 2061, 3295, 5031
Sfr274I CTCGAG 1 cut(s) 4503
Sfr303I CCGCGG 1 cut(s) 1492
SfuI TTCGAA 1 cut(s) 3989
SgrBI CCGCGG 1 cut(s) 1492
SinI GGWCC 4 cut(s) 172, 461, 598, 1458
SlaI CTCGAG 1 cut(s) 4503
SmiMI CAYNNNNRTG 8 cut(s) 761, 1418, 2510, 3585, 3791, 3953, 4244, 4716
SseBI AGGCCT 2 cut(s) 3431, 4509
SsiI CCGC 7 cut(s) 241, 1215, 1489, 1491, 3796, 4005, 4460
SspI AATATT 3 cut(s) 1504, 3812, 4895
StuI AGGCCT 2 cut(s) 3431, 4509
StyD4I CCNGG 7 cut(s) 174, 699, 933, 1033, 2266, 2798, 3268
StyI CCWWGG 5 cut(s) 1694, 2131, 3287, 3819, 4800
TaiI ACGT 5 cut(s) 2596, 2694, 2932, 3795, 4217
TatI WGTACW 3 cut(s) 340, 4734, 4750
TseFI GTSAC 4 cut(s) 1858, 2018, 3419, 3513
Tsp45I GTSAC 4 cut(s) 1858, 2018, 3419, 3513
TspGWI ACGGA 3 cut(s) 453, 1215, 4456
Van91I CCANNNNNTGG 2 cut(s) 1040, 4637
Vha464I CTTAAG 2 cut(s) 2923, 3425
VpaK11BI GGWCC 4 cut(s) 172, 461, 598, 1458
XapI RAATTY 8 cut(s) 800, 990, 2622, 2965, 3475, 3772, 3918, 4144
XceI RCATGY 3 cut(s) 321, 1759, 4652
XcmI CCANNNNNNNNNTGG 1 cut(s) 2020
XhoI CTCGAG 1 cut(s) 4503
XmaJI CCTAGG 1 cut(s) 1694
XmiI GTMKAC 3 cut(s) 397, 1365, 1708
XmnI GAANNNNTTC 8 cut(s) 812, 963, 2483, 3151, 3918, 4281, 4330, 5001
Zsp2I ATGCAT 2 cut(s) 216, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.