RchiOBHm_Chr2g0104121

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000039
Physical Location & Seq
Forward (+)
15538102 .. 15541790
3689 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47839

Sequence Viewer

Length: 2526 bp
ATGGCGGCGAACCTCGCTGCAGGTGGTGCTATGGGAGTACCATTTAATGCGCTCTATGAAGCCATTAAGCAAGTGGTGGTCAAGACTAGAATGTTTAAACCCCTTGTTGAAGACCTGAGGTCGAAGATAAACGCCTTGAAACCCCTCATCGATGCAATGGAGATCTGCAATATAGCTCTGGGGATTTCAGAGGACGAACTTAGAGATTTCAAAATGCATATGGAGAAGGGACCCAAGCTTATTCTCAAGTGCTCCAATCTTAGTGCATGGAAGACTTACAAAAAGTATAAATACAGTAACCAACTTCTTGAATTGCAGAACTCTCTTCAGAATCTGCTACTTGTACTGTCAGTGCAACAAGCAAGGGATTTGAAGGTGAACTTGGTTGCAACAAGGAATATAGAGACGGTGGTGCAGCGGGTGGATGTGAATGTTGCGGCATTGAGACAACACCAAGAGATGAATGTTACGAATCAATTAAGAAGCATAAGCAGTACCGCGATCCAGCATATTGAAAACCAAGGTGAGGTACAATTTCGAATAGATTGTGAAGCACCTGAACCGCCACGGGTTACAGTTGGATTGGAAGGGCCTTTGATGGAATTGAAGATGAAGCTTCTTAAGAATAATAATGTGTCAATGGTTGTGCTGACTGCTCCCGGAGGATCCGGGAAAACCACATTGGCCACAAAGTTTTGTCAAGATGAGGAAGTGAAAGATATATTCAAGAACAACATCTTCTTTGTCACTGTTTCAAAGAAGGCAAACTTCAACCTTATTGTACAAGGACTACATCAACGCAAGGGTACCGAAATACCTACTTTCGAAGAAGAAGCAACTGCCCTCAAGTGGTTGCATAAGTATCTGAAGGAAGAAGGACAGACACCTGTACTGTTGGTCCTGGATGATGTTTGGCCTGAATCAAAATCTCTTCTTGAAATTTTTGATGAAGTGAAAGGGCCGAATTTCAAGATTTTGGTGACATCAAGATCAAAATTTGCAGGATATGGTTCTCTATACCATCTAAAATCATTGAATGAGGCAGATGCAATGACTCTTTTCCGTAATTCAGCATCCCTGGGAGATGGGAGCTCTAGTGTTCAATGGACCAAACTTATTACAAAGATAGTAAAGCGCTGTAAGGGACATCCACTTGCCATTAAAGTGGTCGGAAAATCTCTTCGTGGACAATCTTTTGAGTTCTGGAAGAAAAGAGAAATGGAGTGGTCTAAAGGTGATTCTATTATTGATTCTGAAACTGAAGTGCTTCGTTCCCTTCAAAGTAGCTTCGATGCCTTGGATGAAAAAGGAGCTATCATAAAGGAATGTTTTTTAGACCTTGGTTCATTTCCTGAAGACCAAAGAATTCCTGTTGCTGCTCTAATTGATATGTGGGCAGAGTTGCATGAGCTAGATAAAGATTTCCTGGCTGTTACACACCTGCAAGAGCTTACCACACGAAGTCTTGCTGATCTAGTCACAAGGAAAGATGCAGTGGAGGGTGATGAGTACTACAATGAACACTTCGTCACTCAACATGACTTGCTTAGAGAGCTGGCTATCTACCAAACCAAGTTAGATCCAAATAAACAAAGACTGATAATAGGCAACAATGGAGACGATCTACCCAAGTGCTTGAGAGAACAAACGGATCGACCCATCAAGCCTCGCCTATTATCTATCTCCTCTGATGGAGTGTTCTCAACAAGTTTGCACAACATTGAACTAGCAGAGGTTGAAGTTCTGGTTTTGAATTTCGACACAAAGGACTATGCATTTCCCGAATTTGTGGAGAAAATGGACAATCTGAAGGTTCTAATAGTCACAAATAATGGTTTCTTACCTGCTGAGCTGAGAAATTTTCACCTACTGGATTCTTTATCAAATCTGAAGAGAATCAGATTAGAGCGTGTTTCAATTCCTTCCATAACCAAGAACCCCCTACAGTTGAAGAGTCTAAAGAAGATCTCGTTATTCATGTGTAGCATCGGAGAAGCTTTCAATTGTTCCTTCCAAGTTTCACATGCATTTCCAAATTTAGAGGAAATGCATATTGACTATTGCAATGATTTGGTGGAATTACCTGATGAGCTATGCAATCTGGTTCACCTAAAAAAGCTCAGTATCACACACTGTCCTATTCTATCTGCCTTGCCTGAAAAGATTGGAGAGTTGGAAAACTTGGAAGTGTTAAGGCTAAGGTCCTGCACAGATTTAGCAGAATTGCCAAGCTCAACTACCAACCTCAAGAAGTTAAACTTTCTTGACATATCCTATTGCTTGAGCATCAAGGAACTGCCTAAACAGATTGGAGAAATGAGCAGTTTAATTAGGATCAATATGAGAGAATGCTCAAGATTGAAAGCACTGCCTATATCAATTTGTGATCTTGAGAATAATCTAGAGGAAGTGATATGCGATGACGAGACAGAAGAGTTGTGGGAACCCTTCTTACCTAGACTGAACAAAGGAGTAGTAAGGGCTGTCGAGGAAGAATTCAACCTAACTTGGCTCCAAAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

841

Amino Acids

95.43

Weight (kDa)

5.8

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 136 4.5e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 198 - 358 4e-17 NB-ARC domain
LRR_14 PF23598 673 - 784 3.7e-15 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 11, 1449
Acc36I ACCTGC 3 cut(s) 11, 1449, 1852
Acc65I GGTACC 1 cut(s) 806
AccB1I GGYRCC 1 cut(s) 806
AccII CGCG 1 cut(s) 500
AciI CCGC 5 cut(s) 5, 418, 437, 498, 563
AclWI GGATC 6 cut(s) 496, 660, 673, 1574, 1659, 2342
AcoI YGGCCR 1 cut(s) 684
AcsI RAATTY 9 cut(s) 939, 964, 995, 1365, 1753, 1784, 1858, 2035, 2495
AcuI CTGAAG 6 cut(s) 311, 887, 1281, 1374, 1829, 1910
AfaI GTAC 8 cut(s) 39, 345, 496, 531, 783, 808, 891, 1511
AfeI AGCGCT 1 cut(s) 1136
AfiI CCNNNNNNNGG 2 cut(s) 526, 849
AflII CTTAAG 1 cut(s) 620
AhdI GACNNNNNGTC 1 cut(s) 118
AjnI CCWGG 3 cut(s) 900, 1077, 1425
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 665, 697
Alw21I GWGCWC 2 cut(s) 254, 1094
Alw26I GTCTC 4 cut(s) 398, 439, 1611, 2420
AlwI GGATC 6 cut(s) 496, 660, 673, 1574, 1659, 2342
AlwNI CAGNNNCTG 1 cut(s) 334
Aor51HI AGCGCT 1 cut(s) 1136
AoxI GGCC 4 cut(s) 590, 684, 914, 959
ApeKI GCWGC 3 cut(s) 17, 415, 1376
ApoI RAATTY 9 cut(s) 939, 964, 995, 1365, 1753, 1784, 1858, 2035, 2495
Asp700I GAANNNNTTC 1 cut(s) 1266
Asp718I GGTACC 1 cut(s) 806
AspLEI GCGC 2 cut(s) 52, 1137
AspS9I GGNCC 6 cut(s) 230, 590, 898, 959, 1107, 2202
AsuC2I CCSGG 2 cut(s) 660, 670
AsuHPI GGTGA 7 cut(s) 388, 536, 991, 1247, 1514, 1856, 2099
AsuII TTCGAA 2 cut(s) 538, 825
AvaII GGWCC 4 cut(s) 230, 898, 1107, 2202
AxyI CCTNAGG 1 cut(s) 116
BalI TGGCCA 1 cut(s) 686
BamHI GGATCC 1 cut(s) 665
BanI GGYRCC 1 cut(s) 806
BanII GRGCYC 1 cut(s) 1094
BbsI GAAGAC 3 cut(s) 117, 278, 1362
Bbv12I GWGCWC 2 cut(s) 254, 1094
BbvI GCAGC 3 cut(s) 4, 427, 1363
BccI CCATC 5 cut(s) 592, 1029, 1079, 1667, 1685
BciT130I CCWGG 3 cut(s) 902, 1079, 1427
BcnI CCSGG 2 cut(s) 660, 670
BcoDI GTCTC 4 cut(s) 398, 439, 1611, 2420
BfaI CTAG 7 cut(s) 87, 1095, 1412, 1475, 1727, 2402, 2457
BfmI CTRYAG 2 cut(s) 18, 1943
BfoI RGCGCY 1 cut(s) 1138
BfrI CTTAAG 1 cut(s) 620
BfuAI ACCTGC 3 cut(s) 11, 1449, 1852
BglII AGATCT 2 cut(s) 162, 1965
BisI GCNGC 5 cut(s) 6, 18, 416, 438, 1377
BlpI GCTNAGC 1 cut(s) 1848
BlsI GCNGC 5 cut(s) 7, 19, 417, 439, 1378
BmcAI AGTACT 1 cut(s) 1511
Bme1390I CCNGG 5 cut(s) 660, 670, 902, 1079, 1427
Bme18I GGWCC 4 cut(s) 230, 898, 1107, 2202
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgT120I GGNCC 6 cut(s) 230, 590, 898, 959, 1107, 2202
BmiI GGNNCC 6 cut(s) 231, 232, 667, 808, 2445, 2513
BmrFI CCNGG 5 cut(s) 660, 670, 902, 1079, 1427
BmsI GCATC 7 cut(s) 142, 1036, 1082, 1282, 1480, 1995, 2295
BpiI GAAGAC 3 cut(s) 117, 278, 1362
Bpu10I CCTNAGC 1 cut(s) 2198
Bpu1102I GCTNAGC 1 cut(s) 1848
Bpu14I TTCGAA 2 cut(s) 538, 825
BpuEI CTTGAG 7 cut(s) 230, 830, 1657, 2231, 2302, 2338, 2411
BpuMI CCSGG 2 cut(s) 660, 670
Bsa29I ATCGAT 1 cut(s) 150
BsaJI CCNNGG 6 cut(s) 520, 566, 1077, 1078, 1296, 1339
BsaXI ACNNNNNCTCC 2 cut(s) 1081, 1111
Bsc4I CCNNNNNNNGG 2 cut(s) 526, 849
Bse1I ACTGG 1 cut(s) 1875
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 3 cut(s) 162, 1056, 2071
BseBI CCWGG 3 cut(s) 902, 1079, 1427
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 6 cut(s) 520, 566, 1077, 1078, 1296, 1339
BseGI GGATG 5 cut(s) 430, 910, 1073, 1147, 1306
BseLI CCNNNNNNNGG 2 cut(s) 526, 849
BseMI GCAATG 3 cut(s) 162, 1056, 2071
BseMII CTCAG 4 cut(s) 107, 1839, 1844, 2134
BseNI ACTGG 1 cut(s) 1875
BseRI GAGGAG 1 cut(s) 1675
BseXI GCAGC 3 cut(s) 4, 427, 1363
BsgI GTGCAG 2 cut(s) 434, 2191
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 4 cut(s) 592, 686, 916, 961
BshNI GGYRCC 1 cut(s) 806
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 254, 1094
BsiSI CCGG 2 cut(s) 660, 669
BslFI GGGAC 2 cut(s) 243, 1158
BslI CCNNNNNNNGG 2 cut(s) 526, 849
BsmAI GTCTC 4 cut(s) 398, 439, 1611, 2420
BsmBI CGTCTC 2 cut(s) 398, 1611
BsmFI GGGAC 2 cut(s) 243, 1158
BsmI GAATGC 1 cut(s) 2354
BsnI GGCC 4 cut(s) 592, 686, 916, 961
Bsp119I TTCGAA 2 cut(s) 538, 825
Bsp1286I GDGCHC 2 cut(s) 254, 1094
Bsp1407I TGTACA 1 cut(s) 781
Bsp1720I GCTNAGC 1 cut(s) 1848
BspACI CCGC 5 cut(s) 5, 418, 437, 498, 563
BspANI GGCC 4 cut(s) 592, 686, 916, 961
BspCNI CTCAG 4 cut(s) 108, 1840, 1845, 2133
BspDI ATCGAT 1 cut(s) 150
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 6 cut(s) 231, 232, 667, 808, 2445, 2513
BspMAI CTGCAG 1 cut(s) 22
BspMI ACCTGC 3 cut(s) 11, 1449, 1852
BspPI GGATC 6 cut(s) 496, 660, 673, 1574, 1659, 2342
BspT104I TTCGAA 2 cut(s) 538, 825
BspT107I GGYRCC 1 cut(s) 806
BspTI CTTAAG 1 cut(s) 620
BsrDI GCAATG 3 cut(s) 162, 1056, 2071
BsrGI TGTACA 1 cut(s) 781
BsrI ACTGG 1 cut(s) 1875
BssECI CCNNGG 6 cut(s) 520, 566, 1077, 1078, 1296, 1339
BssT1I CCWWGG 3 cut(s) 520, 1296, 1339
Bst2UI CCWGG 3 cut(s) 902, 1079, 1427
Bst4CI ACNGT 8 cut(s) 296, 348, 409, 577, 751, 894, 1947, 2135
Bst6I CTCTTC 6 cut(s) 330, 936, 1185, 1886, 1946, 2427
BstAFI CTTAAG 1 cut(s) 620
BstAPI GCANNNNNTGC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 781
BstBI TTCGAA 2 cut(s) 538, 825
BstC8I GCNNGC 1 cut(s) 1557
BstDEI CTNAG 8 cut(s) 116, 200, 260, 1547, 1848, 1853, 2120, 2198
BstDSI CCRYGG 1 cut(s) 566
BstF5I GGATG 5 cut(s) 430, 910, 1073, 1147, 1306
BstFNI CGCG 1 cut(s) 500
BstH2I RGCGCY 1 cut(s) 1138
BstHHI GCGC 2 cut(s) 52, 1137
BstMAI GTCTC 4 cut(s) 398, 439, 1611, 2420
BstMWI GCNNNNNNNGC 3 cut(s) 14, 26, 1552
BstNI CCWGG 3 cut(s) 902, 1079, 1427
BstNSI RCATGY 1 cut(s) 2027
BstSCI CCNGG 5 cut(s) 658, 668, 900, 1077, 1425
BstSFI CTRYAG 2 cut(s) 18, 1943
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 3 cut(s) 4, 427, 1363
BstV2I GAAGAC 3 cut(s) 117, 278, 1362
BstX2I RGATCY 4 cut(s) 162, 665, 1579, 1965
BstXI CCANNNNNNTGG 1 cut(s) 1165
BstYI RGATCY 4 cut(s) 162, 665, 1579, 1965
Bsu15I ATCGAT 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 116
BsuRI GGCC 4 cut(s) 592, 686, 916, 961
BsuTUI ATCGAT 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 566
BtgZI GCGATG 1 cut(s) 2433
BtsCI GGATG 5 cut(s) 430, 910, 1073, 1147, 1306
BtsI GCAGTG 2 cut(s) 1500, 2366
BtsIMutI CAGTG 5 cut(s) 357, 747, 1500, 2131, 2366
BveI ACCTGC 3 cut(s) 11, 1449, 1852
Cac8I GCNNGC 1 cut(s) 1557
CaiI CAGNNNCTG 1 cut(s) 334
CfoI GCGC 2 cut(s) 52, 1137
Cfr13I GGNCC 6 cut(s) 230, 590, 898, 959, 1107, 2202
ClaI ATCGAT 1 cut(s) 150
Csp6I GTAC 8 cut(s) 38, 344, 495, 530, 782, 807, 890, 1510
CviAII CATG 5 cut(s) 267, 1406, 1538, 1978, 2024
CviQI GTAC 8 cut(s) 38, 344, 495, 530, 782, 807, 890, 1510
DdeI CTNAG 8 cut(s) 116, 200, 260, 1547, 1848, 1853, 2120, 2198
DraI TTTAAA 1 cut(s) 97
DriI GACNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 6 cut(s) 330, 936, 1185, 1886, 1946, 2427
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 6 cut(s) 330, 936, 1185, 1886, 1946, 2427
Ecl136II GAGCTC 1 cut(s) 1092
Eco130I CCWWGG 3 cut(s) 520, 1296, 1339
Eco24I GRGCYC 1 cut(s) 1094
Eco47I GGWCC 4 cut(s) 230, 898, 1107, 2202
Eco47III AGCGCT 1 cut(s) 1136
Eco53kI GAGCTC 1 cut(s) 1092
Eco57I CTGAAG 6 cut(s) 311, 887, 1281, 1374, 1829, 1910
Eco81I CCTNAGG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 1092
EcoO109I RGGNCCY 3 cut(s) 230, 590, 2202
EcoRI GAATTC 2 cut(s) 1365, 2495
EcoRII CCWGG 3 cut(s) 900, 1077, 1425
EcoT14I CCWWGG 3 cut(s) 520, 1296, 1339
EcoT22I ATGCAT 4 cut(s) 219, 1777, 2029, 2052
EcoT38I GRGCYC 1 cut(s) 1094
ErhI CCWWGG 3 cut(s) 520, 1296, 1339
Esp3I CGTCTC 2 cut(s) 398, 1611
FaeI CATG 5 cut(s) 270, 1409, 1541, 1981, 2027
FalI AAGNNNNNCTT 6 cut(s) 365, 397, 752, 784, 2243, 2275
FaqI GGGAC 2 cut(s) 243, 1158
FatI CATG 5 cut(s) 266, 1405, 1537, 1977, 2023
FauI CCCGC 1 cut(s) 411
FauNDI CATATG 1 cut(s) 219
Fnu4HI GCNGC 5 cut(s) 6, 18, 416, 438, 1377
FokI GGATG 5 cut(s) 437, 917, 1060, 1134, 1313
FriOI GRGCYC 1 cut(s) 1094
Fsp4HI GCNGC 5 cut(s) 6, 18, 416, 438, 1377
FspBI CTAG 7 cut(s) 87, 1095, 1412, 1475, 1727, 2402, 2457
GlaI GCGC 2 cut(s) 51, 1136
GluI GCNGC 5 cut(s) 6, 18, 416, 438, 1377
HaeII RGCGCY 1 cut(s) 1138
HaeIII GGCC 4 cut(s) 592, 686, 916, 961
HapII CCGG 2 cut(s) 660, 669
HhaI GCGC 2 cut(s) 52, 1137
Hin1II CATG 5 cut(s) 270, 1409, 1541, 1981, 2027
Hin6I GCGC 2 cut(s) 50, 1135
HinP1I GCGC 2 cut(s) 50, 1135
HindIII AAGCTT 3 cut(s) 236, 614, 1995
HinfI GANTC 9 cut(s) 331, 472, 920, 1054, 1238, 1250, 1874, 1896, 1954
HpaII CCGG 2 cut(s) 660, 669
HphI GGTGA 7 cut(s) 388, 536, 991, 1247, 1514, 1856, 2099
Hpy166II GTNNAC 3 cut(s) 379, 1187, 2107
Hpy8I GTNNAC 3 cut(s) 379, 1187, 2107
HpyCH4III ACNGT 8 cut(s) 296, 348, 409, 577, 751, 894, 1947, 2135
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 26, 1552
HpyF3I CTNAG 8 cut(s) 116, 200, 260, 1547, 1848, 1853, 2120, 2198
Hsp92II CATG 5 cut(s) 270, 1409, 1541, 1981, 2027
HspAI GCGC 2 cut(s) 50, 1135
KflI GGGWCCC 1 cut(s) 230
KpnI GGTACC 1 cut(s) 810
LmnI GCTCC 5 cut(s) 257, 661, 1089, 1310, 2517
Lsp1109I GCAGC 3 cut(s) 4, 427, 1363
LweI GCATC 7 cut(s) 142, 1036, 1082, 1282, 1480, 1995, 2295
MaeI CTAG 7 cut(s) 87, 1095, 1412, 1475, 1727, 2402, 2457
MaeIII GTNAC 9 cut(s) 296, 466, 571, 745, 979, 1432, 1477, 1528, 1822
MfeI CAATTG 1 cut(s) 2002
MflI RGATCY 4 cut(s) 162, 665, 1579, 1965
MhlI GDGCHC 2 cut(s) 254, 1094
MlsI TGGCCA 1 cut(s) 686
MluNI TGGCCA 1 cut(s) 686
MlyI GAGTC 2 cut(s) 1048, 1963
MmeI TCCRAC 3 cut(s) 559, 1150, 2154
Mox20I TGGCCA 1 cut(s) 686
Mph1103I ATGCAT 4 cut(s) 219, 1777, 2029, 2052
MroXI GAANNNNTTC 1 cut(s) 1266
MscI TGGCCA 1 cut(s) 686
MseI TTAA 9 cut(s) 45, 66, 96, 479, 621, 1161, 2192, 2255, 2327
MslI CAYNNNNRTG 1 cut(s) 1163
Msp20I TGGCCA 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 418
MspCI CTTAAG 1 cut(s) 620
MspI CCGG 2 cut(s) 660, 669
MspR9I CCNGG 5 cut(s) 660, 670, 902, 1079, 1427
MssI GTTTAAAC 1 cut(s) 97
MunI CAATTG 1 cut(s) 2002
Mva1269I GAATGC 1 cut(s) 2354
MvaI CCWGG 3 cut(s) 902, 1079, 1427
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 3 cut(s) 14, 26, 1552
NciI CCSGG 2 cut(s) 660, 670
NdeI CATATG 1 cut(s) 219
NlaIII CATG 5 cut(s) 270, 1409, 1541, 1981, 2027
NlaIV GGNNCC 6 cut(s) 231, 232, 667, 808, 2445, 2513
NmuCI GTSAC 5 cut(s) 745, 979, 1477, 1528, 1822
NsiI ATGCAT 4 cut(s) 219, 1777, 2029, 2052
NspI RCATGY 1 cut(s) 2027
NspV TTCGAA 2 cut(s) 538, 825
PaqCI CACCTGC 2 cut(s) 11, 1449
PasI CCCWGGG 1 cut(s) 1078
PctI GAATGC 1 cut(s) 2354
PdmI GAANNNNTTC 1 cut(s) 1266
PfeI GAWTC 7 cut(s) 331, 472, 920, 1238, 1250, 1874, 1896
PfoI TCCNGGA 3 cut(s) 658, 668, 900
PkrI GCNGC 5 cut(s) 7, 19, 417, 439, 1378
PleI GAGTC 2 cut(s) 1048, 1962
PmeI GTTTAAAC 1 cut(s) 97
PpsI GAGTC 2 cut(s) 1048, 1962
PpuMI RGGWCCY 2 cut(s) 230, 2202
Psp124BI GAGCTC 1 cut(s) 1094
Psp5II RGGWCCY 2 cut(s) 230, 2202
Psp6I CCWGG 3 cut(s) 900, 1077, 1425
PspGI CCWGG 3 cut(s) 900, 1077, 1425
PspN4I GGNNCC 6 cut(s) 231, 232, 667, 808, 2445, 2513
PspPI GGNCC 6 cut(s) 230, 590, 898, 959, 1107, 2202
PspPPI RGGWCCY 2 cut(s) 230, 2202
PsrI GAACNNNNNNTAC 2 cut(s) 2436, 2468
PstI CTGCAG 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 334
PsuI RGATCY 4 cut(s) 162, 665, 1579, 1965
RsaI GTAC 8 cut(s) 39, 345, 496, 531, 783, 808, 891, 1511
RsaNI GTAC 8 cut(s) 38, 344, 495, 530, 782, 807, 890, 1510
RseI CAYNNNNRTG 1 cut(s) 1163
SacI GAGCTC 1 cut(s) 1094
SaqAI TTAA 9 cut(s) 45, 66, 96, 479, 621, 1161, 2192, 2255, 2327
SatI GCNGC 5 cut(s) 6, 18, 416, 438, 1377
Sau96I GGNCC 6 cut(s) 230, 590, 898, 959, 1107, 2202
ScaI AGTACT 1 cut(s) 1511
SchI GAGTC 2 cut(s) 1048, 1963
ScrFI CCNGG 5 cut(s) 660, 670, 902, 1079, 1427
SduI GDGCHC 2 cut(s) 254, 1094
SfaNI GCATC 7 cut(s) 142, 1036, 1082, 1282, 1480, 1995, 2295
SfcI CTRYAG 2 cut(s) 18, 1943
SfuI TTCGAA 2 cut(s) 538, 825
SinI GGWCC 4 cut(s) 230, 898, 1107, 2202
SmiMI CAYNNNNRTG 1 cut(s) 1163
SmlI CTYRAG 8 cut(s) 245, 620, 845, 1636, 2246, 2281, 2353, 2390
SmoI CTYRAG 8 cut(s) 245, 620, 845, 1636, 2246, 2281, 2353, 2390
SsiI CCGC 5 cut(s) 5, 418, 437, 498, 563
SspMI CTAG 7 cut(s) 87, 1095, 1412, 1475, 1727, 2402, 2457
SstI GAGCTC 1 cut(s) 1094
StyD4I CCNGG 5 cut(s) 658, 668, 900, 1077, 1425
StyI CCWWGG 3 cut(s) 520, 1296, 1339
TaaI ACNGT 8 cut(s) 296, 348, 409, 577, 751, 894, 1947, 2135
TaqI TCGA 8 cut(s) 122, 150, 538, 825, 1290, 1654, 1758, 2487
TatI WGTACW 4 cut(s) 343, 781, 889, 1509
TauI GCSGC 2 cut(s) 8, 440
TfiI GAWTC 7 cut(s) 331, 472, 920, 1238, 1250, 1874, 1896
Tru1I TTAA 9 cut(s) 45, 66, 96, 479, 621, 1161, 2192, 2255, 2327
Tru9I TTAA 9 cut(s) 45, 66, 96, 479, 621, 1161, 2192, 2255, 2327
TscAI CASTG 5 cut(s) 357, 754, 1500, 2138, 2373
TseFI GTSAC 5 cut(s) 745, 979, 1477, 1528, 1822
TseI GCWGC 3 cut(s) 17, 415, 1376
Tsp45I GTSAC 5 cut(s) 745, 979, 1477, 1528, 1822
TspDTI ATGAA 8 cut(s) 72, 476, 626, 963, 1317, 1335, 1533, 1966
TspGWI ACGGA 2 cut(s) 1052, 1664
TspRI CASTG 5 cut(s) 357, 754, 1500, 2138, 2373
Vha464I CTTAAG 1 cut(s) 620
VpaK11BI GGWCC 4 cut(s) 230, 898, 1107, 2202
XapI RAATTY 9 cut(s) 939, 964, 995, 1365, 1753, 1784, 1858, 2035, 2495
XbaI TCTAGA 1 cut(s) 2401
XceI RCATGY 1 cut(s) 2027
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XmnI GAANNNNTTC 1 cut(s) 1266
XspI CTAG 7 cut(s) 87, 1095, 1412, 1475, 1727, 2402, 2457
ZrmI AGTACT 1 cut(s) 1511
Zsp2I ATGCAT 4 cut(s) 219, 1777, 2029, 2052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.