Rroxscaffold_2G00139000

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000039
Physical Location & Seq
Reverse (-)
77249969 .. 77254307
4339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139000.1

Sequence Viewer

Length: 1974 bp
ATGGAAGTGGTTACCACAATTGTAGGGGCTGCTCTAGGAGCAGCGTTCCAGCAGCTCTTTGATAGTGTTTTAAAGGCTAACAGAAAAGCTACAATGTTTGATTCCGTCTTCAAGGATCTCGATCTCACGCTCCGGTCTTATCAAGAATTGGTTAAAGATATAGAACAAGTTGAAAATTTTCAACCCTTGCCGGGCCTGCTAGATGGTTTAAAAACGAAGATGGAGGAGGGCAGCCAGCTTGTTGACAAGTGCTCCAATATTCATCCCTTGTTGAAGATTAAAAAGAAGACATATGTTGGAAAACTTCGTGCATTGGATGAATCTCTTCAACGACTCTTCTTCATATTGAAATGGCAAGTAGACATCAATCTGGGGAACAGGGTGAGCAATTTGCAGAGGTCCGTGGACCGAATTGATAGGAACGTGGCGGTGCAAAACCGAACTGAAAATCAAATCAGAGGTTGGTGCGTGGTACCCAATCCTCCGTCATTTACTGTTGGATTGGATAAGCCTTTGAAGGATTTGAAGATGAAGTTTCTAAATGATGATCAAGTGTCAATGCTTGTGCTCACTGCTCCTGGAGGATGCGGGAAAACCACTTTGGCTCAGATGTTCTGTCGAGATGAGGAAGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAATGAAGCCCAACTTATCCCAAATTGTACAAGAGCTGTATCATGGAACGGGTGCCCGGGCCCCCGATTTCCAAAATGACGCAACTGCAGTTAGCTGGCTGCAGCAATTTCTGAAGCAAACAGGACAAAATCCTATGTTGTTGGTCCTGGATGATGTTTGGGCTGGATCAGAATTCATTCTTGAGAAGTTCTATGAATTAAAAATGACAAATTACAAGATATTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTATGTAGAATGTTTGAACGATGAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGAGACAGGAGCTCTTATATTTCAGAAGATCTTCCAAGAAAGATAGTAGAGCGTTGTATAGGATTTCCGCTTGCAATTACAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGCGATTTGGCAAAAAAGAGTATTGGAATGGTCTAGAGGTTCTTCTGTTCTTGAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGAAGCTTAGATGCTTTGGACGAAGAGAAGTCTCTTATCAGGGAATGCTTCATAGACCTTGGTTTATTTCCTGAAGATAAAAGAATCTCAGCTACTCTCCTCATTGATATGTGGGCAGAGTTATATGGCATAAATGAGGACATTTCGTCCATTGTGAACCTCTATGAACTTGCCTCTCGGAGTTTGGTTAATCTGGTACTCACAAGGAATGACAAGGATGGCAATGGCTATTACACTGAACACTTTGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCAACAGATTAGTCGGGAACCACTACAACTGAGAATGAGACTTATGATTGACGTAAGTGGAGACAACTTTCCTATGTGGTGGAAAGAACAAAAGTATCAATCCCTCATAGCTCGCCTTGTATCTATCTCAACAGACGAAGAGTTCTCATCTAAATGGCAAAACTTGCCACTACCAGAAGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTGGAGGAAATGAATAAACTGAAGACTCTAGTACTCACAAGTTCAGGTATCGGTCCTGCTGAGTTGAATAACTTTCAACTAGTTGATTCGAAACCAAATCTAAAGAGAATCAGACTAGAACGAATTTCATTTCCTTCAATAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAGATTTCTCTATTCATGTGTCGCGTTGGCCAAGGTTTTGGTAACGGTTCCATACATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

657

Amino Acids

75.28

Weight (kDa)

6.71

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 121 2.5e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 171 - 330 1.9e-15 NB-ARC domain
AAA_22 PF13401 186 - 288 6.5e-08 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 907
Acc36I ACCTGC 1 cut(s) 1845
Acc65I GGTACC 1 cut(s) 472
AccB1I GGYRCC 2 cut(s) 472, 725
AccB7I CCANNNNNTGG 1 cut(s) 926
AccI GTMKAC 1 cut(s) 360
AccII CGCG 1 cut(s) 1907
AciI CCGC 4 cut(s) 428, 588, 1067, 1101
AclWI GGATC 2 cut(s) 123, 847
AcoI YGGCCR 1 cut(s) 1911
AcsI RAATTY 4 cut(s) 175, 641, 845, 1833
AcuI CTGAAG 3 cut(s) 806, 1290, 1751
AfaI GTAC 4 cut(s) 474, 702, 1395, 1743
AfiI CCNNNNNNNGG 2 cut(s) 191, 926
AhdI GACNNNNNGTC 1 cut(s) 1342
AhlI ACTAGT 1 cut(s) 1789
AjnI CCWGG 2 cut(s) 577, 819
AjuI GAANNNNNNNTTGG 4 cut(s) 584, 616, 913, 945
AluBI AGCT 9 cut(s) 55, 89, 238, 709, 768, 1011, 1203, 1289, 1589
AluI AGCT 9 cut(s) 55, 89, 238, 709, 768, 1011, 1203, 1289, 1589
Alw21I GWGCWC 3 cut(s) 254, 570, 1013
Alw26I GTCTC 4 cut(s) 996, 1233, 1510, 1533
AlwI GGATC 2 cut(s) 123, 847
Ama87I CYCGRG 1 cut(s) 729
AoxI GGCC 3 cut(s) 193, 732, 1911
ApaI GGGCCC 1 cut(s) 736
ApeKI GCWGC 8 cut(s) 29, 41, 52, 231, 772, 775, 989, 1105
ApoI RAATTY 4 cut(s) 175, 641, 845, 1833
Asp700I GAANNNNTTC 4 cut(s) 177, 324, 849, 1029
Asp718I GGTACC 1 cut(s) 472
AspS9I GGNCC 7 cut(s) 193, 399, 406, 732, 733, 817, 1763
AsuC2I CCSGG 3 cut(s) 192, 730, 731
AsuHPI GGTGA 3 cut(s) 394, 910, 974
AsuII TTCGAA 1 cut(s) 1799
AvaI CYCGRG 1 cut(s) 729
AvaII GGWCC 4 cut(s) 399, 406, 817, 1763
AxyI CCTNAGG 1 cut(s) 997
BaeGI GKGCMC 2 cut(s) 730, 736
BaeI ACNNNNGTAYC 2 cut(s) 1556, 1589
BalI TGGCCA 1 cut(s) 1913
BanI GGYRCC 2 cut(s) 472, 725
BanII GRGCYC 2 cut(s) 736, 1013
BbsI GAAGAC 4 cut(s) 100, 293, 649, 1739
Bbv12I GWGCWC 3 cut(s) 254, 570, 1013
BbvI GCAGC 8 cut(s) 16, 53, 64, 243, 759, 787, 1001, 1117
BccI CCATC 3 cut(s) 197, 214, 1409
BcgI CGANNNNNNTGC 2 cut(s) 947, 981
BciT130I CCWGG 2 cut(s) 579, 821
BciVI GTATCC 1 cut(s) 1869
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 3 cut(s) 192, 730, 731
BcoDI GTCTC 4 cut(s) 996, 1233, 1510, 1533
BcuI ACTAGT 1 cut(s) 1789
BfaI CTAG 6 cut(s) 35, 200, 1146, 1739, 1790, 1826
BfmI CTRYAG 2 cut(s) 759, 773
BfuAI ACCTGC 1 cut(s) 1845
BfuI GTATCC 1 cut(s) 1869
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 8 cut(s) 30, 42, 53, 232, 773, 776, 990, 1106
BlsI GCNGC 8 cut(s) 31, 43, 54, 233, 774, 777, 991, 1107
BmcAI AGTACT 1 cut(s) 1743
Bme1390I CCNGG 5 cut(s) 192, 579, 730, 731, 821
Bme18I GGWCC 4 cut(s) 399, 406, 817, 1763
BmeRI GACNNNNNGTC 1 cut(s) 1342
BmeT110I CYCGRG 1 cut(s) 729
BmgT120I GGNCC 7 cut(s) 193, 399, 406, 732, 733, 817, 1763
BmiI GGNNCC 6 cut(s) 474, 727, 734, 735, 1497, 1932
BmrFI CCNGG 5 cut(s) 192, 579, 730, 731, 821
BmsI GCATC 4 cut(s) 575, 955, 1001, 1198
BpiI GAAGAC 4 cut(s) 100, 293, 649, 1739
BpmI CTGGAG 1 cut(s) 600
Bpu10I CCTNAGC 1 cut(s) 1463
Bpu14I TTCGAA 1 cut(s) 1799
BpuEI CTTGAG 2 cut(s) 875, 1184
BpuMI CCSGG 3 cut(s) 192, 730, 731
BsaBI GATNNNNATC 1 cut(s) 120
BsaJI CCNNGG 4 cut(s) 402, 729, 1255, 1914
BsaWI WCCGGW 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 926
Bse21I CCTNAGG 1 cut(s) 997
Bse3DI GCAATG 3 cut(s) 658, 975, 1426
Bse8I GATNNNNATC 1 cut(s) 120
BseBI CCWGG 2 cut(s) 579, 821
BseDI CCNNGG 4 cut(s) 402, 729, 1255, 1914
BseGI GGATG 6 cut(s) 262, 322, 590, 829, 992, 1420
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 191, 926
BseMI GCAATG 3 cut(s) 658, 975, 1426
BseMII CTCAG 5 cut(s) 620, 1299, 1499, 1695, 1761
BseRI GAGGAG 2 cut(s) 239, 1286
BseSI GKGCMC 2 cut(s) 730, 736
BseXI GCAGC 8 cut(s) 16, 53, 64, 243, 759, 787, 1001, 1117
Bsh1236I CGCG 1 cut(s) 1907
BshFI GGCC 3 cut(s) 195, 734, 1913
BshNI GGYRCC 2 cut(s) 472, 725
BsiHKAI GWGCWC 3 cut(s) 254, 570, 1013
BsiHKCI CYCGRG 1 cut(s) 729
BsiSI CCGG 3 cut(s) 133, 191, 730
BslI CCNNNNNNNGG 2 cut(s) 191, 926
BsmAI GTCTC 4 cut(s) 996, 1233, 1510, 1533
BsmI GAATGC 1 cut(s) 1247
BsnI GGCC 3 cut(s) 195, 734, 1913
BsoBI CYCGRG 1 cut(s) 729
Bsp119I TTCGAA 1 cut(s) 1799
Bsp120I GGGCCC 1 cut(s) 732
Bsp1286I GDGCHC 5 cut(s) 254, 570, 730, 736, 1013
Bsp1407I TGTACA 1 cut(s) 700
Bsp143I GATC 6 cut(s) 115, 121, 547, 839, 1027, 1091
BspACI CCGC 4 cut(s) 428, 588, 1067, 1101
BspANI GGCC 3 cut(s) 195, 734, 1913
BspCNI CTCAG 5 cut(s) 619, 1298, 1500, 1696, 1762
BspFNI CGCG 1 cut(s) 1907
BspLI GGNNCC 6 cut(s) 474, 727, 734, 735, 1497, 1932
BspMAI CTGCAG 2 cut(s) 763, 777
BspMI ACCTGC 1 cut(s) 1845
BspPI GGATC 2 cut(s) 123, 847
BspT104I TTCGAA 1 cut(s) 1799
BspT107I GGYRCC 2 cut(s) 472, 725
BsrDI GCAATG 3 cut(s) 658, 975, 1426
BsrGI TGTACA 1 cut(s) 700
BssECI CCNNGG 4 cut(s) 402, 729, 1255, 1914
BssMI GATC 6 cut(s) 115, 121, 547, 839, 1027, 1091
BssT1I CCWWGG 2 cut(s) 1255, 1914
Bst2UI CCWGG 2 cut(s) 579, 821
Bst4CI ACNGT 5 cut(s) 496, 670, 1081, 1869, 1930
Bst6I CTCTTC 4 cut(s) 330, 341, 1215, 1611
BstAPI GCANNNNNTGC 2 cut(s) 1459, 1642
BstAUI TGTACA 1 cut(s) 700
BstBI TTCGAA 1 cut(s) 1799
BstC8I GCNNGC 6 cut(s) 197, 236, 770, 1071, 1103, 1591
BstDEI CTNAG 8 cut(s) 606, 997, 1204, 1285, 1463, 1508, 1704, 1770
BstDSI CCRYGG 1 cut(s) 402
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 6 cut(s) 262, 322, 590, 829, 992, 1420
BstFNI CGCG 1 cut(s) 1907
BstKTI GATC 6 cut(s) 118, 124, 550, 842, 1030, 1094
BstMAI GTCTC 4 cut(s) 996, 1233, 1510, 1533
BstMBI GATC 6 cut(s) 115, 121, 547, 839, 1027, 1091
BstMWI GCNNNNNNNGC 4 cut(s) 38, 196, 1459, 1642
BstNI CCWGG 2 cut(s) 579, 821
BstNSI RCATGY 1 cut(s) 1462
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 5 cut(s) 190, 577, 728, 729, 819
BstSFI CTRYAG 2 cut(s) 759, 773
BstSLI GKGCMC 2 cut(s) 730, 736
BstUI CGCG 1 cut(s) 1907
BstV1I GCAGC 8 cut(s) 16, 53, 64, 243, 759, 787, 1001, 1117
BstV2I GAAGAC 4 cut(s) 100, 293, 649, 1739
BstX2I RGATCY 2 cut(s) 115, 1027
BstXI CCANNNNNNTGG 1 cut(s) 1921
BstYI RGATCY 2 cut(s) 115, 1027
Bsu36I CCTNAGG 1 cut(s) 997
BsuI GTATCC 1 cut(s) 1869
BsuRI GGCC 3 cut(s) 195, 734, 1913
BtgI CCRYGG 1 cut(s) 402
BtsCI GGATG 6 cut(s) 262, 322, 590, 829, 992, 1420
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 2 cut(s) 570, 1431
BveI ACCTGC 1 cut(s) 1845
Cac8I GCNNGC 6 cut(s) 197, 236, 770, 1071, 1103, 1591
Cfr13I GGNCC 7 cut(s) 193, 399, 406, 732, 733, 817, 1763
Cfr9I CCCGGG 1 cut(s) 729
CseI GACGC 1 cut(s) 761
Csp6I GTAC 4 cut(s) 473, 701, 1394, 1742
CviAII CATG 6 cut(s) 716, 986, 1175, 1454, 1459, 1899
CviQI GTAC 4 cut(s) 473, 701, 1394, 1742
DdeI CTNAG 8 cut(s) 606, 997, 1204, 1285, 1463, 1508, 1704, 1770
DpnI GATC 6 cut(s) 117, 123, 549, 841, 1029, 1093
DpnII GATC 6 cut(s) 115, 121, 547, 839, 1027, 1091
DraI TTTAAA 2 cut(s) 72, 210
DrdI GACNNNNNNGTC 1 cut(s) 907
DriI GACNNNNNGTC 1 cut(s) 1342
DseDI GACNNNNNNGTC 1 cut(s) 907
EaeI YGGCCR 1 cut(s) 1911
Eam1104I CTCTTC 4 cut(s) 330, 341, 1215, 1611
Eam1105I GACNNNNNGTC 1 cut(s) 1342
EarI CTCTTC 4 cut(s) 330, 341, 1215, 1611
Ecl136II GAGCTC 1 cut(s) 1011
Eco130I CCWWGG 2 cut(s) 1255, 1914
Eco24I GRGCYC 2 cut(s) 736, 1013
Eco47I GGWCC 4 cut(s) 399, 406, 817, 1763
Eco53kI GAGCTC 1 cut(s) 1011
Eco57I CTGAAG 3 cut(s) 806, 1290, 1751
Eco81I CCTNAGG 1 cut(s) 997
Eco88I CYCGRG 1 cut(s) 729
Eco91I GGTNACC 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 1011
EcoO109I RGGNCCY 1 cut(s) 733
EcoO65I GGTNACC 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 845
EcoRII CCWGG 2 cut(s) 577, 819
EcoT14I CCWWGG 2 cut(s) 1255, 1914
EcoT38I GRGCYC 2 cut(s) 736, 1013
ErhI CCWWGG 2 cut(s) 1255, 1914
FaeI CATG 6 cut(s) 719, 989, 1178, 1457, 1462, 1902
FalI AAGNNNNNCTT 4 cut(s) 671, 703, 898, 930
FatI CATG 6 cut(s) 715, 985, 1174, 1453, 1458, 1898
FauI CCCGC 2 cut(s) 581, 1094
FauNDI CATATG 1 cut(s) 292
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 8 cut(s) 30, 42, 53, 232, 773, 776, 990, 1106
FokI GGATG 6 cut(s) 249, 329, 597, 836, 979, 1427
FriOI GRGCYC 2 cut(s) 736, 1013
Fsp4HI GCNGC 8 cut(s) 30, 42, 53, 232, 773, 776, 990, 1106
FspBI CTAG 6 cut(s) 35, 200, 1146, 1739, 1790, 1826
GluI GCNGC 8 cut(s) 30, 42, 53, 232, 773, 776, 990, 1106
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 3 cut(s) 195, 734, 1913
HapII CCGG 3 cut(s) 133, 191, 730
HgaI GACGC 1 cut(s) 761
Hin1II CATG 6 cut(s) 719, 989, 1178, 1457, 1462, 1902
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 1201
HinfI GANTC 8 cut(s) 101, 320, 333, 1166, 1281, 1735, 1796, 1818
HpaII CCGG 3 cut(s) 133, 191, 730
HphI GGTGA 3 cut(s) 394, 910, 974
Hpy166II GTNNAC 4 cut(s) 244, 361, 406, 1354
Hpy188III TCNNGA 9 cut(s) 119, 143, 620, 646, 854, 1146, 1163, 1268, 1493
Hpy8I GTNNAC 4 cut(s) 244, 361, 406, 1354
HpyAV CCTTC 2 cut(s) 511, 1854
HpyCH4III ACNGT 5 cut(s) 496, 670, 1081, 1869, 1930
HpyCH4IV ACGT 2 cut(s) 423, 1530
HpyCH4V TGCA 8 cut(s) 311, 394, 433, 761, 775, 968, 989, 1073
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 196, 1459, 1642
HpyF3I CTNAG 8 cut(s) 606, 997, 1204, 1285, 1463, 1508, 1704, 1770
HpySE526I ACGT 2 cut(s) 423, 1530
Hsp92II CATG 6 cut(s) 719, 989, 1178, 1457, 1462, 1902
KpnI GGTACC 1 cut(s) 476
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 6 cut(s) 115, 121, 547, 839, 1027, 1091
LmnI GCTCC 5 cut(s) 38, 135, 257, 580, 1008
Lsp1109I GCAGC 8 cut(s) 16, 53, 64, 243, 759, 787, 1001, 1117
LweI GCATC 4 cut(s) 575, 955, 1001, 1198
MaeI CTAG 6 cut(s) 35, 200, 1146, 1739, 1790, 1826
MaeII ACGT 2 cut(s) 423, 1530
MaeIII GTNAC 5 cut(s) 10, 664, 898, 1444, 1924
MalI GATC 6 cut(s) 117, 123, 549, 841, 1029, 1093
MboI GATC 6 cut(s) 115, 121, 547, 839, 1027, 1091
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 2 cut(s) 115, 1027
MhlI GDGCHC 5 cut(s) 254, 570, 730, 736, 1013
MlsI TGGCCA 1 cut(s) 1913
MluNI TGGCCA 1 cut(s) 1913
MlyI GAGTC 3 cut(s) 327, 1175, 1729
MmeI TCCRAC 4 cut(s) 277, 478, 1066, 1504
Mox20I TGGCCA 1 cut(s) 1913
MroXI GAANNNNTTC 4 cut(s) 177, 324, 849, 1029
MscI TGGCCA 1 cut(s) 1913
MseI TTAA 6 cut(s) 71, 153, 209, 279, 872, 1386
MslI CAYNNNNRTG 2 cut(s) 1304, 1630
Msp20I TGGCCA 1 cut(s) 1913
MspI CCGG 3 cut(s) 133, 191, 730
MspR9I CCNGG 5 cut(s) 192, 579, 730, 731, 821
MunI CAATTG 1 cut(s) 18
Mva1269I GAATGC 1 cut(s) 1247
MvaI CCWGG 2 cut(s) 579, 821
MvnI CGCG 1 cut(s) 1907
MwoI GCNNNNNNNGC 4 cut(s) 38, 196, 1459, 1642
NciI CCSGG 3 cut(s) 192, 730, 731
NdeI CATATG 1 cut(s) 292
NdeII GATC 6 cut(s) 115, 121, 547, 839, 1027, 1091
NlaIII CATG 6 cut(s) 719, 989, 1178, 1457, 1462, 1902
NlaIV GGNNCC 6 cut(s) 474, 727, 734, 735, 1497, 1932
NmuCI GTSAC 1 cut(s) 898
NspI RCATGY 1 cut(s) 1462
NspV TTCGAA 1 cut(s) 1799
PctI GAATGC 1 cut(s) 1247
PdmI GAANNNNTTC 4 cut(s) 177, 324, 849, 1029
PfeI GAWTC 5 cut(s) 101, 320, 1281, 1796, 1818
PflMI CCANNNNNTGG 1 cut(s) 926
PfoI TCCNGGA 2 cut(s) 577, 819
PkrI GCNGC 8 cut(s) 31, 43, 54, 233, 774, 777, 991, 1107
PleI GAGTC 3 cut(s) 327, 1174, 1729
PpsI GAGTC 3 cut(s) 327, 1174, 1729
Psp124BI GAGCTC 1 cut(s) 1013
Psp6I CCWGG 2 cut(s) 577, 819
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 2 cut(s) 577, 819
PspN4I GGNNCC 6 cut(s) 474, 727, 734, 735, 1497, 1932
PspOMI GGGCCC 1 cut(s) 732
PspPI GGNCC 7 cut(s) 193, 399, 406, 732, 733, 817, 1763
PstI CTGCAG 2 cut(s) 763, 777
PsuI RGATCY 2 cut(s) 115, 1027
RsaI GTAC 4 cut(s) 474, 702, 1395, 1743
RsaNI GTAC 4 cut(s) 473, 701, 1394, 1742
RseI CAYNNNNRTG 2 cut(s) 1304, 1630
SacI GAGCTC 1 cut(s) 1013
SaqAI TTAA 6 cut(s) 71, 153, 209, 279, 872, 1386
SatI GCNGC 8 cut(s) 30, 42, 53, 232, 773, 776, 990, 1106
Sau3AI GATC 6 cut(s) 115, 121, 547, 839, 1027, 1091
Sau96I GGNCC 7 cut(s) 193, 399, 406, 732, 733, 817, 1763
ScaI AGTACT 1 cut(s) 1743
SchI GAGTC 3 cut(s) 327, 1175, 1729
ScrFI CCNGG 5 cut(s) 192, 579, 730, 731, 821
SduI GDGCHC 5 cut(s) 254, 570, 730, 736, 1013
SfaNI GCATC 4 cut(s) 575, 955, 1001, 1198
SfcI CTRYAG 2 cut(s) 759, 773
SfuI TTCGAA 1 cut(s) 1799
SinI GGWCC 4 cut(s) 399, 406, 817, 1763
SmaI CCCGGG 1 cut(s) 731
SmiMI CAYNNNNRTG 2 cut(s) 1304, 1630
SmlI CTYRAG 2 cut(s) 854, 1163
SmoI CTYRAG 2 cut(s) 854, 1163
SpeI ACTAGT 1 cut(s) 1789
SrfI GCCCGGGC 1 cut(s) 731
SsiI CCGC 4 cut(s) 428, 588, 1067, 1101
SspI AATATT 1 cut(s) 259
SspMI CTAG 6 cut(s) 35, 200, 1146, 1739, 1790, 1826
SstI GAGCTC 1 cut(s) 1013
StyD4I CCNGG 5 cut(s) 190, 577, 728, 729, 819
StyI CCWWGG 2 cut(s) 1255, 1914
TaaI ACNGT 5 cut(s) 496, 670, 1081, 1869, 1930
TaiI ACGT 2 cut(s) 426, 1533
TaqI TCGA 3 cut(s) 120, 619, 1799
TaqII GACCGA 2 cut(s) 423, 1751
TatI WGTACW 2 cut(s) 700, 1741
TfiI GAWTC 5 cut(s) 101, 320, 1281, 1796, 1818
Tru1I TTAA 6 cut(s) 71, 153, 209, 279, 872, 1386
Tru9I TTAA 6 cut(s) 71, 153, 209, 279, 872, 1386
TscAI CASTG 2 cut(s) 577, 1438
TseFI GTSAC 1 cut(s) 898
TseI GCWGC 8 cut(s) 29, 41, 52, 231, 772, 775, 989, 1105
Tsp45I GTSAC 1 cut(s) 898
TspGWI ACGGA 3 cut(s) 94, 391, 474
TspMI CCCGGG 1 cut(s) 729
TspRI CASTG 2 cut(s) 577, 1438
Van91I CCANNNNNTGG 1 cut(s) 926
VpaK11BI GGWCC 4 cut(s) 399, 406, 817, 1763
XapI RAATTY 4 cut(s) 175, 641, 845, 1833
XbaI TCTAGA 1 cut(s) 1145
XceI RCATGY 1 cut(s) 1462
XcmI CCANNNNNNNNNTGG 1 cut(s) 1116
XmaI CCCGGG 1 cut(s) 729
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 4 cut(s) 177, 324, 849, 1029
XspI CTAG 6 cut(s) 35, 200, 1146, 1739, 1790, 1826
ZrmI AGTACT 1 cut(s) 1743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.