RchiOBHm_Chr2g0104241

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000039
Physical Location & Seq
Forward (+)
15646593 .. 15650516
3924 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47851

Sequence Viewer

Length: 2565 bp
ATGGCCAATCCAGCGGATGCTTTCGCCGGGGCTGCTGCAGGATTTCTATTCAGTGTGCTCTATGATGGCCTCAAGGAGCTGACCACCAAGTATGTGCTGTTCAAACACGTCCTCAAAAGCGTCAAATCCACACTCGAATCTCTGGAGCAAGTGGTTCAAGACATAGCACGACTTAACGAGGATCTGGATCGCCCGAAGGAAGAACTAGCGAGTCTAGAAACCCAACTAGAGAAGGGCAAAGTGCTCGTTGCCAAGTGCGGCAAGGTTCGTGTGTGGAGTGTGTACAAGAAGTACAAGTACTCCAGGAAACTTCTTGGATGGAATGACAGTCTTAAAGAACAGTTGGAGATGCTCAAAGTTCATGCAATAAGAGATGGGAAGGAGACCCTAACAACGGTGAAGAGAACAAAGAAGACTGTGAGCCAAATCGAATCGACCAGTTTCAATATAAAGGAGGGAGTGGAGCAAATTGAGACCAACACTGCAAATCTACAGGTGGTGGTGAGCCGAGTGGAAGCGAGAGTTTCGAATATCGAGGCTGCAGGGATAAAGCAGATACAACCCGAAGCTAAAATTGAAGCTTGGTGTGCAGTACCTGAACTTCCAACTCTTACAGTTGGATTGGATGGGCCTTTGAACGAACTGAAGATGAAGTTGTTCAACAATGGAGTGTCAATGGTTGTGCTGACCGCTCCCGGAGGATGTGGAAAAACCACTTTGGCAACAAAGTTCTGTCAAGATGAGCAAGTCCAAGAGAAATTCAAGAACAATATATTTTTTGTCACTGTTTCCAAAATGCCAAACTTCTACCTTATTGTACAAGAGCTATACCAAAGCAAGGGTAACCAGGCACCTGATTTTAAAGATGATGAAAATGCACTCAAGTGGCTGCAAAAATTTATGAAGGAAGAAGGACAAAACCCTTTACTGTTGGTCTTGGATGATGTTTGGTCTGGATCAGATTCTTTACTTGCGAAGTTTGAATTCAAAATGCAAGATTACAAAGTTTTAGTGACATCAAGATCTGAATTTCCGAGATATGGCTCCTATGCTTTGCAGTCATTGGATTTTGACAATGCAATGAAACTCTTTCATCATGCAGCATCCTTGGGAGAAAAAAGTTCGCATATTCCACTAGAAATTTCAAGACAGGTAGTAGAGGGCTGTAAGGGATTTCCACTTGCTATAACAGTGGCCGGAAAATCACTTTGCGGACAGCCTGTAGAGACTTGGCAGAAAAGACTCATTGAATGGTCCAAAGGTTCTTCGATTCTTGATTCCGAGACTGAATTGCTTCTTCGCCTCCAAACCAGTTTAGATGTCTCGAATAAGGAAATGAGGATGGTCAAAGAATGTTTCTTAGACCTAGGTTCATTTCCTGAAGATCAAAGAATCCCTGTTGCTGCCCTCATTGATATCTGGACTGAGTCTTACGGTTCACTGGATGAAGACATTTTGTGTGTTGCAAACCTCCACCAGATCACAAACCGAAGTCTAGCTAATCTTGTAGTCACAAGGAAGGACAAGCTGCAGGGGGATGGATACTACAGTGAACATTTTGTTACCCAGCATGATCTGCTTAGGGAGCTGGCTATCTATGAGACAAAACAAGAGGACCCCGAACATAGAGAAAGACTAATTGTAAACATATTTGAAGACAATTTTCCCAAGTGGTGGAGCGAACAGAAGTATCAATGCATGAAGGCTCGCTTGTTATCTATCTCAACTGATGGGGCGTTTTCAACAAAATGGCACGACATGGAACTACCAGAAGCTGAGGTTCTAGTCTTGAATTTTAAGACCAAGAACTATCCATTACCCCAATTTGTGGAGAAAATGGTTAAGTTGAAGGTACTGGTAGTCACAAATTATGGTATTGTACCTTCAGAATTAACAAATTTCCAAATACTCTGTACGTCATCAAAGTTGAAGAGAATGAGATTAGAACGTATCTCAATTCCATCCATAGCCAAGCACCCCATGCAATTGTATAGTCTCCAGAAGATTTCTTTATTCATGTGTAATATTGGTCAAGCTTTCAGCAATGGGTCAATCCAATTTTCACATGCATGGCCAAATCTAGTGGAAGTGAACATTGACTATTGCAATGATTTGGTGGCATTGCCTGCTGACACCAGTGATTTGATTCATCTAAAGAAGCTCAGCATCACCAACTGTCATAAGCTACTTGCCTTGCCTGAAGATATTGGAAAGCTGGCCAAATTAGAATTGTTGAGGCTAAGGTCTTGTACAGACTTAGCAAAGTTGCCGGGTTCGACTGGGAACCTCAAGAAGCTAAAATCTCTTGATATATCTTACTGCTTCAGCATTAAGGAGTTGCCTGAAGACTTTGGTGAATTGAGCAGCTTAAGAGAGCTTAACATGAGAGACTGCTCAAGATTGCAAGAGCTGCCTCTATCAGTTTCAAATCTGAAGCATTTAAAAGTGATTGGTGATGATGAGACACAAAGTTTATGGCAACCCTTCTTACCTATCAAAGGCACACACATAGTAGTGGCCAAAGAGAATATCAACCTAAATTGGCTCCCCAAGTTTCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

854

Amino Acids

96.88

Weight (kDa)

6.79

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 138 3.8e-27 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 211 - 343 8.1e-17 NB-ARC domain
LRR_14 PF23598 713 - 817 3.5e-12 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 850
AccB7I CCANNNNNTGG 2 cut(s) 1674, 1828
AccBSI CCGCTC 1 cut(s) 692
AciI CCGC 4 cut(s) 14, 258, 690, 1212
AclWI GGATC 3 cut(s) 189, 195, 964
AcoI YGGCCR 5 cut(s) 3, 1194, 2072, 2217, 2517
AcsI RAATTY 7 cut(s) 758, 896, 983, 1028, 1140, 1792, 1897
AcuI CTGAAG 7 cut(s) 665, 1401, 1869, 2220, 2308, 2364, 2453
AfaI GTAC 9 cut(s) 284, 293, 299, 594, 819, 1854, 1881, 1915, 2251
AfiI CCNNNNNNNGG 6 cut(s) 394, 838, 1040, 1674, 1828, 2498
AflII CTTAAG 1 cut(s) 2368
AflIII ACRYGT 1 cut(s) 106
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 2 cut(s) 302, 846
AleI CACNNNNGTG 1 cut(s) 883
Alw21I GWGCWC 2 cut(s) 60, 246
Alw26I GTCTC 9 cut(s) 377, 467, 1220, 1277, 1327, 1595, 2000, 2382, 2456
AlwI GGATC 3 cut(s) 189, 195, 964
AlwNI CAGNNNCTG 2 cut(s) 596, 1775
AoxI GGCC 7 cut(s) 3, 67, 629, 1194, 2072, 2217, 2517
ApeKI GCWGC 9 cut(s) 32, 35, 539, 889, 1100, 1403, 1528, 2364, 2410
ApoI RAATTY 7 cut(s) 758, 896, 983, 1028, 1140, 1792, 1897
Asp700I GAANNNNTTC 2 cut(s) 656, 1293
AspA2I CCTAGG 1 cut(s) 1366
AspS9I GGNCC 3 cut(s) 629, 1254, 1615
AsuC2I CCSGG 3 cut(s) 28, 696, 2271
AsuHPI GGTGA 5 cut(s) 409, 514, 2161, 2366, 2465
AsuII TTCGAA 1 cut(s) 527
AvaII GGWCC 2 cut(s) 1254, 1615
AvrII CCTAGG 1 cut(s) 1366
BaeI ACNNNNGTAYC 4 cut(s) 1534, 1567, 1673, 1706
BalI TGGCCA 4 cut(s) 5, 2074, 2219, 2519
BanI GGYRCC 1 cut(s) 850
BarI GAAGNNNNNNTAC 6 cut(s) 281, 313, 585, 617, 1867, 1899
BbsI GAAGAC 4 cut(s) 419, 1455, 1662, 2352
Bbv12I GWGCWC 2 cut(s) 60, 246
BbvCI CCTCAGC 1 cut(s) 1776
BbvI GCAGC 9 cut(s) 19, 22, 526, 876, 1112, 1390, 1515, 2376, 2397
BccI CCATC 8 cut(s) 59, 312, 368, 620, 1336, 1532, 1724, 1969
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 2 cut(s) 304, 848
BciVI GTATCC 1 cut(s) 1535
BcnI CCSGG 3 cut(s) 28, 696, 2271
BcoDI GTCTC 9 cut(s) 377, 467, 1220, 1277, 1327, 1595, 2000, 2382, 2456
BfaI CTAG 8 cut(s) 206, 215, 227, 1136, 1367, 1496, 1784, 2081
BfmI CTRYAG 6 cut(s) 36, 491, 540, 1221, 1529, 1546
BfrI CTTAAG 1 cut(s) 2368
BfuI GTATCC 1 cut(s) 1535
BglII AGATCT 1 cut(s) 1022
BlnI CCTAGG 1 cut(s) 1366
BlpI GCTNAGC 1 cut(s) 2162
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 5 cut(s) 28, 304, 696, 848, 2271
Bme18I GGWCC 2 cut(s) 1254, 1615
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 629, 1254, 1615
BmiI GGNNCC 5 cut(s) 852, 1045, 1617, 2285, 2546
BmrFI CCNGG 5 cut(s) 28, 304, 696, 848, 2271
BmrI ACTGGG 1 cut(s) 2289
BmsI GCATC 4 cut(s) 7, 339, 1112, 2175
BmuI ACTGGG 1 cut(s) 2289
BpiI GAAGAC 4 cut(s) 419, 1455, 1662, 2352
BpmI CTGGAG 3 cut(s) 164, 286, 1982
Bpu10I CCTNAGC 3 cut(s) 1580, 1776, 2240
Bpu1102I GCTNAGC 1 cut(s) 2162
Bpu14I TTCGAA 1 cut(s) 527
BpuEI CTTGAG 4 cut(s) 56, 866, 2273, 2380
BpuMI CCSGG 3 cut(s) 28, 696, 2271
BsaBI GATNNNNATC 1 cut(s) 186
BsaI GGTCTC 2 cut(s) 377, 467
BsaJI CCNNGG 3 cut(s) 27, 1107, 1366
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 6 cut(s) 394, 838, 1040, 1674, 1828, 2498
Bse1I ACTGG 6 cut(s) 438, 1311, 1446, 1860, 2136, 2284
Bse3DI GCAATG 4 cut(s) 1086, 2050, 2113, 2120
Bse8I GATNNNNATC 1 cut(s) 186
BseBI CCWGG 2 cut(s) 304, 848
BseDI CCNNGG 3 cut(s) 27, 1107, 1366
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 6 cut(s) 394, 838, 1040, 1674, 1828, 2498
BseMI GCAATG 4 cut(s) 1086, 2050, 2113, 2120
BseMII CTCAG 3 cut(s) 1416, 1767, 2176
BseNI ACTGG 6 cut(s) 438, 1311, 1446, 1860, 2136, 2284
BseXI GCAGC 9 cut(s) 19, 22, 526, 876, 1112, 1390, 1515, 2376, 2397
BseYI CCCAGC 1 cut(s) 1566
BsgI GTGCAG 1 cut(s) 609
BshFI GGCC 7 cut(s) 5, 69, 631, 1196, 2074, 2219, 2519
BshNI GGYRCC 1 cut(s) 850
BsiHKAI GWGCWC 2 cut(s) 60, 246
BsiSI CCGG 4 cut(s) 27, 696, 1197, 2270
BslI CCNNNNNNNGG 6 cut(s) 394, 838, 1040, 1674, 1828, 2498
BsmAI GTCTC 9 cut(s) 377, 467, 1220, 1277, 1327, 1595, 2000, 2382, 2456
BsnI GGCC 7 cut(s) 5, 69, 631, 1196, 2074, 2219, 2519
Bso31I GGTCTC 2 cut(s) 377, 467
Bsp119I TTCGAA 1 cut(s) 527
Bsp1286I GDGCHC 2 cut(s) 60, 246
Bsp1407I TGTACA 3 cut(s) 282, 817, 2249
Bsp143I GATC 7 cut(s) 181, 187, 956, 1022, 1384, 1479, 1573
Bsp1720I GCTNAGC 1 cut(s) 2162
BspACI CCGC 4 cut(s) 14, 258, 690, 1212
BspANI GGCC 7 cut(s) 5, 69, 631, 1196, 2074, 2219, 2519
BspCNI CTCAG 3 cut(s) 1417, 1768, 2175
BspLI GGNNCC 5 cut(s) 852, 1045, 1617, 2285, 2546
BspMAI CTGCAG 3 cut(s) 40, 544, 1533
BspPI GGATC 3 cut(s) 189, 195, 964
BspT104I TTCGAA 1 cut(s) 527
BspT107I GGYRCC 1 cut(s) 850
BspTI CTTAAG 1 cut(s) 2368
BspTNI GGTCTC 2 cut(s) 377, 467
BsrBI CCGCTC 1 cut(s) 692
BsrDI GCAATG 4 cut(s) 1086, 2050, 2113, 2120
BsrGI TGTACA 3 cut(s) 282, 817, 2249
BsrI ACTGG 6 cut(s) 438, 1311, 1446, 1860, 2136, 2284
BssECI CCNNGG 3 cut(s) 27, 1107, 1366
BssMI GATC 7 cut(s) 181, 187, 956, 1022, 1384, 1479, 1573
BssT1I CCWWGG 2 cut(s) 1107, 1366
Bst2UI CCWGG 2 cut(s) 304, 848
Bst6I CTCTTC 2 cut(s) 395, 1925
BstAFI CTTAAG 1 cut(s) 2368
BstAPI GCANNNNNTGC 4 cut(s) 1576, 1981, 2126, 2410
BstAUI TGTACA 3 cut(s) 282, 817, 2249
BstBI TTCGAA 1 cut(s) 527
BstC8I GCNNGC 4 cut(s) 1590, 1708, 2127, 2217
BstDEI CTNAG 7 cut(s) 1360, 1425, 1580, 1776, 2162, 2240, 2257
BstEII GGTNACC 1 cut(s) 842
BstENI CCTNNNNNAGG 1 cut(s) 2496
BstKTI GATC 7 cut(s) 184, 190, 959, 1025, 1387, 1482, 1576
BstMAI GTCTC 9 cut(s) 377, 467, 1220, 1277, 1327, 1595, 2000, 2382, 2456
BstMBI GATC 7 cut(s) 181, 187, 956, 1022, 1384, 1479, 1573
BstMWI GCNNNNNNNGC 8 cut(s) 11, 32, 587, 1576, 1585, 1981, 2126, 2410
BstNI CCWGG 2 cut(s) 304, 848
BstNSI RCATGY 1 cut(s) 2069
BstPI GGTNACC 1 cut(s) 842
BstSCI CCNGG 5 cut(s) 26, 302, 694, 846, 2269
BstSFI CTRYAG 6 cut(s) 36, 491, 540, 1221, 1529, 1546
BstV1I GCAGC 9 cut(s) 19, 22, 526, 876, 1112, 1390, 1515, 2376, 2397
BstV2I GAAGAC 4 cut(s) 419, 1455, 1662, 2352
BstX2I RGATCY 2 cut(s) 181, 1022
BstYI RGATCY 2 cut(s) 181, 1022
BsuI GTATCC 1 cut(s) 1535
BsuRI GGCC 7 cut(s) 5, 69, 631, 1196, 2074, 2219, 2519
BtrI CACGTC 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 7 cut(s) 58, 480, 783, 1197, 1439, 1555, 2143
Cac8I GCNNGC 4 cut(s) 1590, 1708, 2127, 2217
CaiI CAGNNNCTG 2 cut(s) 596, 1775
Cfr13I GGNCC 3 cut(s) 629, 1254, 1615
CseI GACGC 1 cut(s) 109
Csp6I GTAC 9 cut(s) 283, 292, 298, 593, 818, 1853, 1880, 1914, 2250
CspCI CAANNNNNGTGG 2 cut(s) 2064, 2099
CviQI GTAC 9 cut(s) 283, 292, 298, 593, 818, 1853, 1880, 1914, 2250
DdeI CTNAG 7 cut(s) 1360, 1425, 1580, 1776, 2162, 2240, 2257
DpnI GATC 7 cut(s) 183, 189, 958, 1024, 1386, 1481, 1575
DpnII GATC 7 cut(s) 181, 187, 956, 1022, 1384, 1479, 1573
DraI TTTAAA 2 cut(s) 862, 2442
EaeI YGGCCR 5 cut(s) 3, 1194, 2072, 2217, 2517
Eam1104I CTCTTC 2 cut(s) 395, 1925
EarI CTCTTC 2 cut(s) 395, 1925
Eco130I CCWWGG 2 cut(s) 1107, 1366
Eco31I GGTCTC 2 cut(s) 377, 467
Eco32I GATATC 1 cut(s) 1417
Eco47I GGWCC 2 cut(s) 1254, 1615
Eco57I CTGAAG 7 cut(s) 665, 1401, 1869, 2220, 2308, 2364, 2453
Eco91I GGTNACC 1 cut(s) 842
EcoNI CCTNNNNNAGG 1 cut(s) 2496
EcoO109I RGGNCCY 1 cut(s) 1615
EcoO65I GGTNACC 1 cut(s) 842
EcoRI GAATTC 1 cut(s) 983
EcoRII CCWGG 2 cut(s) 302, 846
EcoRV GATATC 1 cut(s) 1417
EcoT14I CCWWGG 2 cut(s) 1107, 1366
EcoT22I ATGCAT 2 cut(s) 1700, 2071
ErhI CCWWGG 2 cut(s) 1107, 1366
FalI AAGNNNNNCTT 2 cut(s) 1694, 1726
FspBI CTAG 8 cut(s) 206, 215, 227, 1136, 1367, 1496, 1784, 2081
GsaI CCCAGC 1 cut(s) 1570
GsuI CTGGAG 3 cut(s) 164, 286, 1982
HaeIII GGCC 7 cut(s) 5, 69, 631, 1196, 2074, 2219, 2519
HapII CCGG 4 cut(s) 27, 696, 1197, 2270
HgaI GACGC 1 cut(s) 109
HindIII AAGCTT 2 cut(s) 579, 2034
HpaII CCGG 4 cut(s) 27, 696, 1197, 2270
HphI GGTGA 5 cut(s) 409, 514, 2161, 2366, 2465
Hpy166II GTNNAC 5 cut(s) 283, 1439, 1553, 1645, 2092
Hpy188I TCNGA 6 cut(s) 961, 1027, 1035, 1282, 1888, 2433
Hpy8I GTNNAC 5 cut(s) 283, 1439, 1553, 1645, 2092
HpyCH4IV ACGT 3 cut(s) 108, 1916, 1948
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 32, 587, 1576, 1585, 1981, 2126, 2410
HpyF3I CTNAG 7 cut(s) 1360, 1425, 1580, 1776, 2162, 2240, 2257
HpySE526I ACGT 3 cut(s) 108, 1916, 1948
Kzo9I GATC 7 cut(s) 181, 187, 956, 1022, 1384, 1479, 1573
LmnI GCTCC 8 cut(s) 76, 145, 463, 697, 1049, 1585, 1677, 2550
Lsp1109I GCAGC 9 cut(s) 19, 22, 526, 876, 1112, 1390, 1515, 2376, 2397
LweI GCATC 4 cut(s) 7, 339, 1112, 2175
MaeI CTAG 8 cut(s) 206, 215, 227, 1136, 1367, 1496, 1784, 2081
MaeII ACGT 3 cut(s) 108, 1916, 1948
MaeIII GTNAC 6 cut(s) 781, 842, 1012, 1510, 1561, 1861
MalI GATC 7 cut(s) 183, 189, 958, 1024, 1386, 1481, 1575
MbiI CCGCTC 1 cut(s) 692
MboI GATC 7 cut(s) 181, 187, 956, 1022, 1384, 1479, 1573
MfeI CAATTG 1 cut(s) 1985
MflI RGATCY 2 cut(s) 181, 1022
MhlI GDGCHC 2 cut(s) 60, 246
MlsI TGGCCA 4 cut(s) 5, 2074, 2219, 2519
MluNI TGGCCA 4 cut(s) 5, 2074, 2219, 2519
MlyI GAGTC 3 cut(s) 220, 1236, 1436
MmeI TCCRAC 3 cut(s) 324, 598, 629
Mox20I TGGCCA 4 cut(s) 5, 2074, 2219, 2519
Mph1103I ATGCAT 2 cut(s) 1700, 2071
MroXI GAANNNNTTC 2 cut(s) 656, 1293
MscI TGGCCA 4 cut(s) 5, 2074, 2219, 2519
MslI CAYNNNNRTG 3 cut(s) 883, 2068, 2513
Msp20I TGGCCA 4 cut(s) 5, 2074, 2219, 2519
MspA1I CMGCKG 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 2368
MspI CCGG 4 cut(s) 27, 696, 1197, 2270
MspR9I CCNGG 5 cut(s) 28, 304, 696, 848, 2271
MunI CAATTG 1 cut(s) 1985
MvaI CCWGG 2 cut(s) 304, 848
MwoI GCNNNNNNNGC 8 cut(s) 11, 32, 587, 1576, 1585, 1981, 2126, 2410
NciI CCSGG 3 cut(s) 28, 696, 2271
NdeII GATC 7 cut(s) 181, 187, 956, 1022, 1384, 1479, 1573
NlaIV GGNNCC 5 cut(s) 852, 1045, 1617, 2285, 2546
NmeAIII GCCGAG 1 cut(s) 533
NmuCI GTSAC 4 cut(s) 781, 1012, 1510, 1861
NsiI ATGCAT 2 cut(s) 1700, 2071
NspI RCATGY 1 cut(s) 2069
NspV TTCGAA 1 cut(s) 527
OliI CACNNNNGTG 1 cut(s) 883
PdmI GAANNNNTTC 2 cut(s) 656, 1293
PfeI GAWTC 7 cut(s) 137, 431, 962, 1270, 1277, 1392, 2146
PflFI GACNNNGTC 1 cut(s) 1426
PflMI CCANNNNNTGG 2 cut(s) 1674, 1828
PfoI TCCNGGA 2 cut(s) 302, 694
PleI GAGTC 3 cut(s) 219, 1236, 1435
PpsI GAGTC 3 cut(s) 219, 1236, 1435
PpuMI RGGWCCY 1 cut(s) 1615
Psp5II RGGWCCY 1 cut(s) 1615
Psp6I CCWGG 2 cut(s) 302, 846
PspEI GGTNACC 1 cut(s) 842
PspFI CCCAGC 1 cut(s) 1566
PspGI CCWGG 2 cut(s) 302, 846
PspN4I GGNNCC 5 cut(s) 852, 1045, 1617, 2285, 2546
PspPI GGNCC 3 cut(s) 629, 1254, 1615
PspPPI RGGWCCY 1 cut(s) 1615
PsrI GAACNNNNNNTAC 2 cut(s) 83, 115
PstI CTGCAG 3 cut(s) 40, 544, 1533
PstNI CAGNNNCTG 2 cut(s) 596, 1775
PsuI RGATCY 2 cut(s) 181, 1022
PsyI GACNNNGTC 1 cut(s) 1426
RsaI GTAC 9 cut(s) 284, 293, 299, 594, 819, 1854, 1881, 1915, 2251
RsaNI GTAC 9 cut(s) 283, 292, 298, 593, 818, 1853, 1880, 1914, 2250
RseI CAYNNNNRTG 3 cut(s) 883, 2068, 2513
Sau3AI GATC 7 cut(s) 181, 187, 956, 1022, 1384, 1479, 1573
Sau96I GGNCC 3 cut(s) 629, 1254, 1615
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 3 cut(s) 220, 1236, 1436
ScrFI CCNGG 5 cut(s) 28, 304, 696, 848, 2271
SduI GDGCHC 2 cut(s) 60, 246
SfaNI GCATC 4 cut(s) 7, 339, 1112, 2175
SfcI CTRYAG 6 cut(s) 36, 491, 540, 1221, 1529, 1546
SfuI TTCGAA 1 cut(s) 527
SinI GGWCC 2 cut(s) 1254, 1615
SmiMI CAYNNNNRTG 3 cut(s) 883, 2068, 2513
SmlI CTYRAG 5 cut(s) 71, 881, 2288, 2368, 2395
SmoI CTYRAG 5 cut(s) 71, 881, 2288, 2368, 2395
SsiI CCGC 4 cut(s) 14, 258, 690, 1212
SspI AATATT 1 cut(s) 2026
SspMI CTAG 8 cut(s) 206, 215, 227, 1136, 1367, 1496, 1784, 2081
StyD4I CCNGG 5 cut(s) 26, 302, 694, 846, 2269
StyI CCWWGG 2 cut(s) 1107, 1366
TaiI ACGT 3 cut(s) 111, 1919, 1951
TaqI TCGA 8 cut(s) 135, 429, 434, 527, 534, 1268, 1325, 2276
TatI WGTACW 5 cut(s) 282, 291, 297, 817, 2249
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 7 cut(s) 137, 431, 962, 1270, 1277, 1392, 2146
TscAI CASTG 7 cut(s) 58, 487, 790, 1197, 1446, 1555, 2143
TseFI GTSAC 4 cut(s) 781, 1012, 1510, 1861
TseI GCWGC 9 cut(s) 32, 35, 539, 889, 1100, 1403, 1528, 2364, 2410
Tsp45I GTSAC 4 cut(s) 781, 1012, 1510, 1861
TspRI CASTG 7 cut(s) 58, 487, 790, 1197, 1446, 1555, 2143
Tth111I GACNNNGTC 1 cut(s) 1426
Van91I CCANNNNNTGG 2 cut(s) 1674, 1828
Vha464I CTTAAG 1 cut(s) 2368
VpaK11BI GGWCC 2 cut(s) 1254, 1615
XagI CCTNNNNNAGG 1 cut(s) 2496
XapI RAATTY 7 cut(s) 758, 896, 983, 1028, 1140, 1792, 1897
XbaI TCTAGA 1 cut(s) 214
XceI RCATGY 1 cut(s) 2069
XmaJI CCTAGG 1 cut(s) 1366
XmnI GAANNNNTTC 2 cut(s) 656, 1293
XspI CTAG 8 cut(s) 206, 215, 227, 1136, 1367, 1496, 1784, 2081
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 2 cut(s) 1700, 2071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.