FvH4_1g15170

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000039
Physical Location & Seq
Forward (+)
8601655 .. 8606777
5123 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15170.t1

Sequence Viewer

Length: 2502 bp
ATGCAGTATTGGATTGAACAATTCCGTCAATTCGTATATAGCGTGCAAATACTAGTGGTGATCATCAGCGGAGAGGCTTTAGCAGTGGCATTTGGGGCGCTGTGTGATTCTGTTAAGCAAGTCAAGGACCAGATTTTGATGTCCAAACCCCAACTCGGTGATATCAAATCCACTCTGGAGACTCTGCAGCCACTGATCAAGGAGATGGCAGAATGTGAGAAAGAACTGGATGGTCCTGAGGAGGAACTAGAGAATCTTAGAGTGAAAATGGAGGAAGGAGCGGAACTTATTTGGAAATGCTCGAAAGCCAGCGCATTCAAAAAGTATAAATATAGCAGCAGACTTGATGAATTGCGCAAGTCACTTCAGAGGCTGTTGGGGATATTGAAAGTGCAGGGCATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGGAACATTGAGAATGTGGTGCAGAGAATTGAAGGGAATTGCGTGATACAGAACAATCAGTCTGAAAAAATACAAGGGTGGTGTGCTGTGCCTGAGCCTCCAACGATTACGGTTGGATTGGATGTGCCTTTTAGGGAATTGAAGATGAAACTCTTTACTGATGAAGTTTCCATGGTGATAGTTACTGCTCCCGGAGGATGTGGGAAGACTACCCTGGCAACAAAGTTGTGCCAAGATGCAGAAATCAAAGATAAATTCAAGAGCAATATCTTCTTTGTTACTGTATCAAAGAAGGCCAACTTATTTCTGATTGTCCAAGAGCTATATCATCGAAAGGGTGCCCCAGTCCCTGATTTCCAAAGTGAAGTAACAGCAGTTGGCTGGCTGCACCAATTTCTGAAGCAAATAGGACAAAACCCTATGTTGTTTGTACTGGATGATGTCTGGGCTGGATCGGAGTTTATTCTTGAGAAATTTGATGAATTAAAAATGACAAATTACAAGATATTGGTGACATCAAGGTCTGCTTTCCCAAGATTTGGTTCTCCATATTACTTGGAATGTTTGAAAGACGAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGAGACAGGAGCTCTTATATTTCAGAAGATCTTCCGAGAAAGATAGTAGAGCGTTGTATGGGATTTCCACTAGCAATTACAGTTGTTGGAAGATCACTCTGCGGTCAGCCAATAGAGATTTGGCAAAAAAGAGTACTGGAATGGTCTAGAGGTTCTTCTGTTCTTGAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGTAGCTTAGATGTTTTGGATGAAGAGAAGCCTCTTATCAGGGAATGCTTCATAGACCTTGGTTTATTTCCCGAAGATCAAAGAATCCCAGCTACTGTCCTCATTGATATGTGGGCAGAGTTATATGGCATACATGAAGACATTTCGTCAATTTCACACCTCTATGAACTTGCCTCTCAAAGTTTGGCTAATCTGGTGGTCACAAGGAAGGACAACAAGTATAGTGATGGCTATTACACTGAGCACTTCGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCGACAGATGAGTCAGGAACCGCTACAACAAAGAATGAGAGTTAAGATTGACATATGTGGAGACAACCTTCCTATGTGGTGGAAGGAGCAAAAGTATCAATCCCTCATCGCTCGCGTAGTATCTATCTCGACAGATGAAGAGTTCTCATCTACATGGCAAAACTTGCAACTACCAGAAGCAGAGGTTTTAATTTTGAATTTTCAAACAAAGTATTATGCCTTACCTGTGTTCGTAGAGAAAATGAATAAACTGAAGACTCTAGTACTCACCGGTTACAGTACTAGTCCTACCGAGTTGAATAACTTTCAACTAGTTGATTCGATACCAAGTTTAAAGAGAATCAGATTGGAGAGAATTTCAATTCCTTCCATAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTTGCATCGGCCAAGGTTTTGGTAACGGTTCCATACCGATATCAGAGGCGTTTCCAAATGTTGTTGAAATGAATATTGACTACTGCAATGATTTGGTTGAGTTGCCTGCCCAACTCTGTGACCTTATACAACTCAAGAAGCTCAGTGTTACCAATTCTCATAAGCTCTCTGCCTTACCTGAAGACATTGGAAATCTAGTCAATTTAGAGCTGCTGAGGCTTAGGTCTTGTACAGACTTGTCAGAGTTACCCGGCTCTATTAGGAACCTCAAGAAGTTGAACTTTCTTGACATATCGGATTGCTTTAGCATTATGGAATTGCCTGAAGACATTGGAGAACTGTGCAGTTTAGAAAGGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCTTCAGTTTTGGATCTTGAACACTTGAAGGAAGTCACATGCGACGAAGAGACAGAAATATTATGGGAACCCTTCTTACCCTATCTCACACACATGCACATAAAGGTTGTGAAAGAAGATATCAACCTCAATTGGCTCCACAAGTCTCATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

834

Amino Acids

95.3

Weight (kDa)

5.36

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 21 - 147 6.9e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 189 - 345 1.6e-15 NB-ARC domain
LRR_14 PF23598 674 - 750 1.1e-09 Leucine-rich repeat region
LRR_14 PF23598 753 - 802 1.6e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 952, 1536
Acc16I TGCGCA 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 1893
AccB1I GGYRCC 1 cut(s) 770
AccB7I CCANNNNNTGG 1 cut(s) 971
AccBSI CCGCTC 1 cut(s) 281
AccII CGCG 1 cut(s) 1641
AciI CCGC 4 cut(s) 69, 281, 1146, 1547
AclWI GGATC 2 cut(s) 892, 2367
AcoI YGGCCR 1 cut(s) 1960
AcsI RAATTY 4 cut(s) 686, 905, 1723, 1881
AcuI CTGAAG 6 cut(s) 350, 851, 1799, 2151, 2295, 2334
AfaI GTAC 5 cut(s) 864, 1179, 1791, 1807, 2182
AfiI CCNNNNNNNGG 3 cut(s) 155, 971, 1902
AgeI ACCGGT 1 cut(s) 1796
AhdI GACNNNNNGTC 1 cut(s) 1387
AhlI ACTAGT 3 cut(s) 52, 1808, 1837
AjnI CCWGG 1 cut(s) 645
AjuI GAANNNNNNNTTGG 4 cut(s) 404, 436, 958, 990
AluBI AGCT 7 cut(s) 754, 1056, 1248, 1334, 2092, 2116, 2161
AluI AGCT 7 cut(s) 754, 1056, 1248, 1334, 2092, 2116, 2161
Alw21I GWGCWC 3 cut(s) 1058, 1488, 2327
Alw26I GTCTC 6 cut(s) 173, 1041, 1581, 2311, 2390, 2496
AlwI GGATC 2 cut(s) 892, 2367
AlwNI CAGNNNCTG 4 cut(s) 193, 373, 782, 1337
Ama87I CYCGRG 1 cut(s) 2326
AoxI GGCC 2 cut(s) 726, 1960
ApeKI GCWGC 5 cut(s) 187, 336, 817, 1034, 2161
ApoI RAATTY 4 cut(s) 686, 905, 1723, 1881
AsiGI ACCGGT 1 cut(s) 1796
Asp700I GAANNNNTTC 1 cut(s) 1074
AspLEI GCGC 3 cut(s) 100, 314, 357
AspS9I GGNCC 2 cut(s) 127, 233
AsuC2I CCSGG 2 cut(s) 624, 2202
AsuHPI GGTGA 7 cut(s) 70, 170, 442, 619, 955, 1019, 1786
AvaI CYCGRG 1 cut(s) 2326
AvaII GGWCC 2 cut(s) 127, 233
AxyI CCTNAGG 2 cut(s) 237, 1042
BaeGI GKGCMC 1 cut(s) 775
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 1 cut(s) 1058
BbsI GAAGAC 5 cut(s) 644, 1386, 1787, 2139, 2283
Bbv12I GWGCWC 3 cut(s) 1058, 1488, 2327
BbvCI CCTCAGC 1 cut(s) 2165
BbvI GCAGC 5 cut(s) 199, 348, 804, 1046, 2148
BccI CCATC 3 cut(s) 199, 224, 1463
BcgI CGANNNNNNTGC 2 cut(s) 408, 442
BciT130I CCWGG 1 cut(s) 647
BciVI GTATCC 1 cut(s) 1917
BclI TGATCA 2 cut(s) 60, 195
BcnI CCSGG 2 cut(s) 624, 2202
BcoDI GTCTC 6 cut(s) 173, 1041, 1581, 2311, 2390, 2496
BcuI ACTAGT 3 cut(s) 52, 1808, 1837
BfaI CTAG 8 cut(s) 53, 248, 1115, 1191, 1787, 1809, 1838, 2147
BfmI CTRYAG 1 cut(s) 185
BfoI RGCGCY 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 1893
BfuI GTATCC 1 cut(s) 1917
BglII AGATCT 1 cut(s) 1072
BisI GCNGC 5 cut(s) 188, 337, 818, 1035, 2162
BlsI GCNGC 5 cut(s) 189, 338, 819, 1036, 2163
BmcAI AGTACT 3 cut(s) 1179, 1791, 1807
Bme1390I CCNGG 3 cut(s) 624, 647, 2202
Bme18I GGWCC 2 cut(s) 127, 233
BmeRI GACNNNNNGTC 1 cut(s) 1387
BmeT110I CYCGRG 1 cut(s) 2326
BmgT120I GGNCC 2 cut(s) 127, 233
BmiI GGNNCC 6 cut(s) 772, 1545, 1981, 2216, 2415, 2483
BmrFI CCNGG 3 cut(s) 624, 647, 2202
BmrI ACTGGG 1 cut(s) 770
BmsI GCATC 4 cut(s) 658, 1000, 1046, 1965
BmuI ACTGGG 1 cut(s) 770
BpiI GAAGAC 5 cut(s) 644, 1386, 1787, 2139, 2283
BpmI CTGGAG 1 cut(s) 197
Bpu10I CCTNAGC 4 cut(s) 525, 1511, 2165, 2171
BpuEI CTTGAG 4 cut(s) 920, 1229, 2069, 2204
BpuMI CCSGG 2 cut(s) 624, 2202
BsaJI CCNNGG 4 cut(s) 603, 645, 1300, 1963
BsaWI WCCGGW 1 cut(s) 1796
BsaXI ACNNNNNCTCC 2 cut(s) 1604, 1634
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 971, 1902
Bse118I RCCGGY 1 cut(s) 1796
Bse1I ACTGG 4 cut(s) 231, 776, 870, 1185
Bse21I CCTNAGG 2 cut(s) 237, 1042
Bse3DI GCAATG 2 cut(s) 1020, 2044
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 4 cut(s) 603, 645, 1300, 1963
BseGI GGATG 7 cut(s) 235, 412, 559, 635, 874, 1037, 1267
BseLI CCNNNNNNNGG 3 cut(s) 155, 971, 1902
BseMI GCAATG 2 cut(s) 1020, 2044
BseMII CTCAG 5 cut(s) 228, 516, 1473, 2107, 2156
BseNI ACTGG 4 cut(s) 231, 776, 870, 1185
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 5 cut(s) 199, 348, 804, 1046, 2148
BseYI CCCAGC 1 cut(s) 1330
BsgI GTGCAG 4 cut(s) 413, 473, 803, 2314
Bsh1236I CGCG 1 cut(s) 1641
BshFI GGCC 2 cut(s) 728, 1962
BshNI GGYRCC 1 cut(s) 770
BshTI ACCGGT 1 cut(s) 1796
BsiHKAI GWGCWC 3 cut(s) 1058, 1488, 2327
BsiHKCI CYCGRG 1 cut(s) 2326
BsiSI CCGG 3 cut(s) 624, 1797, 2202
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 3 cut(s) 155, 971, 1902
BsmAI GTCTC 6 cut(s) 173, 1041, 1581, 2311, 2390, 2496
BsmFI GGGAC 1 cut(s) 764
BsmI GAATGC 2 cut(s) 314, 1292
BsnI GGCC 2 cut(s) 728, 1962
BsoBI CYCGRG 1 cut(s) 2326
Bsp1286I GDGCHC 4 cut(s) 775, 1058, 1488, 2327
Bsp1407I TGTACA 1 cut(s) 2180
Bsp143I GATC 7 cut(s) 60, 195, 884, 1072, 1136, 1318, 2359
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 4 cut(s) 69, 281, 1146, 1547
BspANI GGCC 2 cut(s) 728, 1962
BspCNI CTCAG 5 cut(s) 229, 517, 1474, 2106, 2157
BspFNI CGCG 1 cut(s) 1641
BspLI GGNNCC 6 cut(s) 772, 1545, 1981, 2216, 2415, 2483
BspMAI CTGCAG 1 cut(s) 189
BspMI ACCTGC 1 cut(s) 1893
BspPI GGATC 2 cut(s) 892, 2367
BspT107I GGYRCC 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 281
BsrDI GCAATG 2 cut(s) 1020, 2044
BsrFI RCCGGY 1 cut(s) 1796
BsrGI TGTACA 1 cut(s) 2180
BsrI ACTGG 4 cut(s) 231, 776, 870, 1185
BssAI RCCGGY 1 cut(s) 1796
BssECI CCNNGG 4 cut(s) 603, 645, 1300, 1963
BssMI GATC 7 cut(s) 60, 195, 884, 1072, 1136, 1318, 2359
BssT1I CCWWGG 3 cut(s) 603, 1300, 1963
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 9 cut(s) 544, 715, 1126, 1339, 1805, 1917, 1979, 2292, 2322
Bst6I CTCTTC 3 cut(s) 1260, 1659, 2388
BstAPI GCANNNNNTGC 2 cut(s) 1507, 1690
BstAUI TGTACA 1 cut(s) 2180
BstC8I GCNNGC 5 cut(s) 44, 310, 815, 1639, 2058
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 7 cut(s) 235, 412, 559, 635, 874, 1037, 1267
BstFNI CGCG 1 cut(s) 1641
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 3 cut(s) 100, 314, 357
BstKTI GATC 7 cut(s) 63, 198, 887, 1075, 1139, 1321, 2362
BstMAI GTCTC 6 cut(s) 173, 1041, 1581, 2311, 2390, 2496
BstMBI GATC 7 cut(s) 60, 195, 884, 1072, 1136, 1318, 2359
BstMWI GCNNNNNNNGC 4 cut(s) 95, 1507, 1690, 2167
BstNI CCWGG 1 cut(s) 647
BstNSI RCATGY 3 cut(s) 1510, 2388, 2443
BstSCI CCNGG 3 cut(s) 622, 645, 2200
BstSFI CTRYAG 1 cut(s) 185
BstSLI GKGCMC 1 cut(s) 775
BstUI CGCG 1 cut(s) 1641
BstV1I GCAGC 5 cut(s) 199, 348, 804, 1046, 2148
BstV2I GAAGAC 5 cut(s) 644, 1386, 1787, 2139, 2283
BstX2I RGATCY 2 cut(s) 1072, 2359
BstXI CCANNNNNNTGG 1 cut(s) 1970
BstYI RGATCY 2 cut(s) 1072, 2359
Bsu36I CCTNAGG 2 cut(s) 237, 1042
BsuI GTATCC 1 cut(s) 1917
BsuRI GGCC 2 cut(s) 728, 1962
BtgI CCRYGG 1 cut(s) 603
BtgZI GCGATG 1 cut(s) 1618
BtsCI GGATG 7 cut(s) 235, 412, 559, 635, 874, 1037, 1267
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 5 cut(s) 90, 191, 1479, 2101, 2327
BveI ACCTGC 1 cut(s) 1893
Cac8I GCNNGC 5 cut(s) 44, 310, 815, 1639, 2058
CaiI CAGNNNCTG 4 cut(s) 193, 373, 782, 1337
CfoI GCGC 3 cut(s) 100, 314, 357
Cfr10I RCCGGY 1 cut(s) 1796
Cfr13I GGNCC 2 cut(s) 127, 233
Csp6I GTAC 5 cut(s) 863, 1178, 1790, 1806, 2181
CspAI ACCGGT 1 cut(s) 1796
CviQI GTAC 5 cut(s) 863, 1178, 1790, 1806, 2181
DpnI GATC 7 cut(s) 62, 197, 886, 1074, 1138, 1320, 2361
DpnII GATC 7 cut(s) 60, 195, 884, 1072, 1136, 1318, 2359
DraI TTTAAA 1 cut(s) 1860
DrdI GACNNNNNNGTC 2 cut(s) 952, 1536
DriI GACNNNNNGTC 1 cut(s) 1387
DseDI GACNNNNNNGTC 2 cut(s) 952, 1536
EaeI YGGCCR 1 cut(s) 1960
Eam1104I CTCTTC 3 cut(s) 1260, 1659, 2388
Eam1105I GACNNNNNGTC 1 cut(s) 1387
EarI CTCTTC 3 cut(s) 1260, 1659, 2388
Ecl136II GAGCTC 1 cut(s) 1056
Eco130I CCWWGG 3 cut(s) 603, 1300, 1963
Eco24I GRGCYC 1 cut(s) 1058
Eco32I GATATC 3 cut(s) 163, 1992, 2467
Eco47I GGWCC 2 cut(s) 127, 233
Eco53kI GAGCTC 1 cut(s) 1056
Eco57I CTGAAG 6 cut(s) 350, 851, 1799, 2151, 2295, 2334
Eco81I CCTNAGG 2 cut(s) 237, 1042
Eco88I CYCGRG 1 cut(s) 2326
EcoICRI GAGCTC 1 cut(s) 1056
EcoRII CCWGG 1 cut(s) 645
EcoRV GATATC 3 cut(s) 163, 1992, 2467
EcoT14I CCWWGG 3 cut(s) 603, 1300, 1963
EcoT38I GRGCYC 1 cut(s) 1058
ErhI CCWWGG 3 cut(s) 603, 1300, 1963
FalI AAGNNNNNCTT 6 cut(s) 716, 748, 943, 975, 2216, 2248
FaqI GGGAC 1 cut(s) 764
FauNDI CATATG 1 cut(s) 1580
FbaI TGATCA 2 cut(s) 60, 195
Fnu4HI GCNGC 5 cut(s) 188, 337, 818, 1035, 2162
FokI GGATG 7 cut(s) 242, 419, 566, 642, 881, 1024, 1274
FriOI GRGCYC 1 cut(s) 1058
Fsp4HI GCNGC 5 cut(s) 188, 337, 818, 1035, 2162
FspBI CTAG 8 cut(s) 53, 248, 1115, 1191, 1787, 1809, 1838, 2147
FspI TGCGCA 1 cut(s) 356
GlaI GCGC 3 cut(s) 99, 313, 356
GluI GCNGC 5 cut(s) 188, 337, 818, 1035, 2162
GsaI CCCAGC 1 cut(s) 1334
GsuI CTGGAG 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 2 cut(s) 728, 1962
HapII CCGG 3 cut(s) 624, 1797, 2202
HhaI GCGC 3 cut(s) 100, 314, 357
Hin6I GCGC 3 cut(s) 98, 312, 355
HinP1I GCGC 3 cut(s) 98, 312, 355
HinfI GANTC 9 cut(s) 107, 181, 253, 1211, 1326, 1537, 1783, 1844, 1866
HpaII CCGG 3 cut(s) 624, 1797, 2202
HphI GGTGA 7 cut(s) 70, 170, 442, 619, 955, 1019, 1786
Hpy99I CGWCG 1 cut(s) 2393
HpyCH4III ACNGT 9 cut(s) 544, 715, 1126, 1339, 1805, 1917, 1979, 2292, 2322
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 1507, 1690, 2167
HspAI GCGC 3 cut(s) 98, 312, 355
Ksp22I TGATCA 2 cut(s) 60, 195
Kzo9I GATC 7 cut(s) 60, 195, 884, 1072, 1136, 1318, 2359
LmnI GCTCC 5 cut(s) 278, 625, 1053, 1612, 2487
Lsp1109I GCAGC 5 cut(s) 199, 348, 804, 1046, 2148
LweI GCATC 4 cut(s) 658, 1000, 1046, 1965
MaeI CTAG 8 cut(s) 53, 248, 1115, 1191, 1787, 1809, 1838, 2147
MalI GATC 7 cut(s) 62, 197, 886, 1074, 1138, 1320, 2361
MbiI CCGCTC 1 cut(s) 281
MboI GATC 7 cut(s) 60, 195, 884, 1072, 1136, 1318, 2359
MfeI CAATTG 1 cut(s) 2476
MflI RGATCY 2 cut(s) 1072, 2359
MhlI GDGCHC 4 cut(s) 775, 1058, 1488, 2327
MlyI GAGTC 4 cut(s) 175, 1220, 1546, 1777
MmeI TCCRAC 4 cut(s) 526, 557, 1111, 1552
MroXI GAANNNNTTC 1 cut(s) 1074
MseI TTAA 5 cut(s) 114, 917, 1569, 1715, 1859
MslI CAYNNNNRTG 4 cut(s) 1349, 1404, 1678, 2438
MspA1I CMGCKG 1 cut(s) 69
MspI CCGG 3 cut(s) 624, 1797, 2202
MspR9I CCNGG 3 cut(s) 624, 647, 2202
MunI CAATTG 1 cut(s) 2476
Mva1269I GAATGC 2 cut(s) 314, 1292
MvaI CCWGG 1 cut(s) 647
MvnI CGCG 1 cut(s) 1641
MwoI GCNNNNNNNGC 4 cut(s) 95, 1507, 1690, 2167
NciI CCSGG 2 cut(s) 624, 2202
NcoI CCATGG 1 cut(s) 603
NdeI CATATG 1 cut(s) 1580
NdeII GATC 7 cut(s) 60, 195, 884, 1072, 1136, 1318, 2359
NlaIV GGNNCC 6 cut(s) 772, 1545, 1981, 2216, 2415, 2483
NmuCI GTSAC 5 cut(s) 360, 943, 1441, 2069, 2380
NsbI TGCGCA 1 cut(s) 356
NspI RCATGY 3 cut(s) 1510, 2388, 2443
PaeR7I CTCGAG 1 cut(s) 2326
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 2 cut(s) 314, 1292
PdmI GAANNNNTTC 1 cut(s) 1074
PfeI GAWTC 5 cut(s) 107, 253, 1326, 1844, 1866
PflMI CCANNNNNTGG 1 cut(s) 971
PfoI TCCNGGA 1 cut(s) 622
PinAI ACCGGT 1 cut(s) 1796
PkrI GCNGC 5 cut(s) 189, 338, 819, 1036, 2163
PleI GAGTC 4 cut(s) 175, 1219, 1545, 1777
PpsI GAGTC 4 cut(s) 175, 1219, 1545, 1777
Psp124BI GAGCTC 1 cut(s) 1058
Psp6I CCWGG 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 1330
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 6 cut(s) 772, 1545, 1981, 2216, 2415, 2483
PspPI GGNCC 2 cut(s) 127, 233
PsrI GAACNNNNNNTAC 2 cut(s) 2406, 2438
PstI CTGCAG 1 cut(s) 189
PstNI CAGNNNCTG 4 cut(s) 193, 373, 782, 1337
PsuI RGATCY 2 cut(s) 1072, 2359
RsaI GTAC 5 cut(s) 864, 1179, 1791, 1807, 2182
RsaNI GTAC 5 cut(s) 863, 1178, 1790, 1806, 2181
RseI CAYNNNNRTG 4 cut(s) 1349, 1404, 1678, 2438
SacI GAGCTC 1 cut(s) 1058
SaqAI TTAA 5 cut(s) 114, 917, 1569, 1715, 1859
SatI GCNGC 5 cut(s) 188, 337, 818, 1035, 2162
Sau3AI GATC 7 cut(s) 60, 195, 884, 1072, 1136, 1318, 2359
Sau96I GGNCC 2 cut(s) 127, 233
ScaI AGTACT 3 cut(s) 1179, 1791, 1807
SchI GAGTC 4 cut(s) 175, 1220, 1546, 1777
ScrFI CCNGG 3 cut(s) 624, 647, 2202
SduI GDGCHC 4 cut(s) 775, 1058, 1488, 2327
SfaNI GCATC 4 cut(s) 658, 1000, 1046, 1965
SfcI CTRYAG 1 cut(s) 185
Sfr274I CTCGAG 1 cut(s) 2326
SinI GGWCC 2 cut(s) 127, 233
SlaI CTCGAG 1 cut(s) 2326
SmiMI CAYNNNNRTG 4 cut(s) 1349, 1404, 1678, 2438
SmlI CTYRAG 5 cut(s) 899, 1208, 2084, 2219, 2326
SmoI CTYRAG 5 cut(s) 899, 1208, 2084, 2219, 2326
SpeI ACTAGT 3 cut(s) 52, 1808, 1837
SsiI CCGC 4 cut(s) 69, 281, 1146, 1547
SspI AATATT 2 cut(s) 2026, 2406
SspMI CTAG 8 cut(s) 53, 248, 1115, 1191, 1787, 1809, 1838, 2147
SstI GAGCTC 1 cut(s) 1058
StyD4I CCNGG 3 cut(s) 622, 645, 2200
StyI CCWWGG 3 cut(s) 603, 1300, 1963
TaaI ACNGT 9 cut(s) 544, 715, 1126, 1339, 1805, 1917, 1979, 2292, 2322
TaqI TCGA 5 cut(s) 302, 763, 1655, 1847, 2327
TatI WGTACW 5 cut(s) 862, 1177, 1789, 1805, 2180
TfiI GAWTC 5 cut(s) 107, 253, 1326, 1844, 1866
Tru1I TTAA 5 cut(s) 114, 917, 1569, 1715, 1859
Tru9I TTAA 5 cut(s) 114, 917, 1569, 1715, 1859
TscAI CASTG 5 cut(s) 90, 198, 1486, 2101, 2327
TseFI GTSAC 5 cut(s) 360, 943, 1441, 2069, 2380
TseI GCWGC 5 cut(s) 187, 336, 817, 1034, 2161
Tsp45I GTSAC 5 cut(s) 360, 943, 1441, 2069, 2380
TspGWI ACGGA 1 cut(s) 14
TspRI CASTG 5 cut(s) 90, 198, 1486, 2101, 2327
Van91I CCANNNNNTGG 1 cut(s) 971
VpaK11BI GGWCC 2 cut(s) 127, 233
XapI RAATTY 4 cut(s) 686, 905, 1723, 1881
XbaI TCTAGA 1 cut(s) 1190
XceI RCATGY 3 cut(s) 1510, 2388, 2443
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XhoI CTCGAG 1 cut(s) 2326
XmnI GAANNNNTTC 1 cut(s) 1074
XspI CTAG 8 cut(s) 53, 248, 1115, 1191, 1787, 1809, 1838, 2147
ZrmI AGTACT 3 cut(s) 1179, 1791, 1807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.