Rh2DG176600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000039
Physical Location & Seq
Forward (+)
15527119 .. 15531276
4158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG176600.1

Sequence Viewer

Length: 2406 bp
ATGGAAGTGGTTACCACAGTTGTAGTGGCTGCTCTAGGAGTAGCGTTCCAGCAGCTCTTTGATAGTGTTATAGAGGCTAACAGAAAAGCTACAATGTTTGATACCGTCTTCAGGGATCTCGATCTCACGCTCCAGTCTTATCAAGAGTTGGTTAAAGGTATAGAACAAGTTGAAAATTTTCAACCCTTGCCGGGCCTGCTAGATGCTTTAAAAACGAAGATGGAGGAGGGCAGTCAGCTTGTTGACAAGTGCTCCAATATTCATCCCTCGTTGAAGATTAAATTGTTGAAAAAGGCGAAATATGTTGGAAAACTTCGTGCATTGGATGGATCTCTTAAACAACACTTCCACATATTGAAATGGCGAGTAGACATCAGTCTGGTGAACAAGGCGAGCAATTTGATTGGTAGGAAACGAACTGAAAATCAAATCAGCGGGTGCGTGGTACCCAATCCTCCGTCATTTACTGTTGGATTGGATAAGCCTTTGAAGGATTTGAAGATGAAGTTTCTAAATGATGATCAAGTGTCAATGCTTGTGCTCACTGCTCCTGGAGGATGGGGGAAAACCACTTTGGCTCAGATGTTCTGTCGAGATGAGGAAGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAATGAAGCCCAACTTATCCCAAATTGTACAAGAGCTGTATCATGGAACGGATGCCAAGGCCCCCGATTTCCAAAATGACGCAACTGTAGTTAGCTGGCTGCAGCAATTTCTGAAGCAAACAGGACAAAATCCTATGTTGTTGGTCCTGGATGATGTTTGGCCTGGATCAGAATTCATTCTTGAGAAGTTCTATGAATTAAAAATGACAAATTACAAGATATTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTATGTAGAATGTTTGAACGATGAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGAGACAGGAGCACATATATTTCAGAAGAACTTCCAAAAAAGATAGTAGAACGTTGTAAGGGATTTCCACTTGGAATTACAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTACTGGAATGGTCTAGAGGTTCTGTTCTTGAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGAAGCTTAGATGCTTTGGATGAAAAGAAGTCTCTTATCAGGGAATGCTTCATAGACCTTGGTTTATTTCCTGAAGATAAAAGAATCTCAGCTACTGTCCTCATTGATATGTGGGCAGAGTTATATGGCATAAATGAGGACATTTCGTCCATTGTGAACCTCTATGAACTTGCCTCTCGGAGTTTGGTTAATCTGGTACTCACAAGGAATGACAACGATGGCAATGGCTATTACACTGAACACTTTGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCAACAGATTAGTCGGGAACCACTACAACTGAGAATGAGACTTATGATTGACGTAAGTGGAGACAACTTTCCTAAGTGGTGGAAAGAACAAAAGTATCAATCCCTCATAGCTCGCCTTGTATCTATCTCAACAGACGAAGAGTTCTCATCTAAATGGCAAAACTTGCCACTACCAGAAGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTGGAGGAAATGAATAAACTGAAGACTCTAGTACTCACAAGTTCAGGTATCGGTCCTGCTGAGTTGAATAACTTTCAACTAGTTGATTCGAAACCAAATCTAAAGAGAATCAGACTAGAACGAATTTCATTTCCTTCATTAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTAGCGTTGGCCAAGGTTTTGGTAACGGTTCCATACATCTATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGTTGCCTGCCGATCTCTGTGACCTTATTCAACTGAAGAAGCTCAGTGTCACCAATTCTCATAAGCTCTCTGTCTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGCTGCTGAGGCTTAGGTGTTGTACAGACTTGTCAGAGTTACCCGGCTCGATTAGGAAGCTCAAGAACTTAATCTTGCTTGACATATCTGATTGCTTTACCATTGAGAAATTGCCTGAAGACATTGGAGAACTGTGCAGTTTAGAAAGGTTCAACATGAGACATTGCTCGAGATTGAATGAGTTGCCTTCTTCAGTTCGGAATCTTAAACAGTTGAAGGAAGTGACATGTGATAAAGAGACAGAAATCTTATGGGAGCCCTTCTTACAAAGGAACATACACATAAGGGTTGTGAAAGAAGATATCAACCTGAATTGGCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

801

Amino Acids

91.52

Weight (kDa)

5.85

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 10 - 118 3.6e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 162 - 321 3.3e-14 NB-ARC domain
AAA_22 PF13401 177 - 268 6.2e-07 AAA domain
LRR_14 PF23598 641 - 725 4.9e-09 Leucine-rich repeat region
LRR_14 PF23598 727 - 775 9.3e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 880
Acc36I ACCTGC 1 cut(s) 1815
Acc65I GGTACC 1 cut(s) 445
AccB1I GGYRCC 1 cut(s) 445
AccB7I CCANNNNNTGG 1 cut(s) 899
AccI GTMKAC 1 cut(s) 369
AciI CCGC 2 cut(s) 435, 1074
AclI AACGTT 1 cut(s) 1024
AclWI GGATC 3 cut(s) 123, 337, 820
AcoI YGGCCR 1 cut(s) 1882
AcsI RAATTY 5 cut(s) 175, 614, 818, 1803, 2074
AcuI CTGAAG 8 cut(s) 94, 779, 1260, 1721, 2027, 2073, 2217, 2256
AfaI GTAC 6 cut(s) 447, 675, 1107, 1365, 1713, 2104
AfiI CCNNNNNNNGG 3 cut(s) 111, 191, 899
AflIII ACRYGT 1 cut(s) 2306
AhdI GACNNNNNGTC 1 cut(s) 1312
AhlI ACTAGT 1 cut(s) 1759
AjnI CCWGG 3 cut(s) 550, 792, 808
AjuI GAANNNNNNNTTGG 4 cut(s) 557, 589, 886, 918
Alw21I GWGCWC 3 cut(s) 254, 543, 986
Alw26I GTCTC 6 cut(s) 969, 1203, 1480, 1503, 2233, 2312
AlwI GGATC 3 cut(s) 123, 337, 820
AlwNI CAGNNNCTG 1 cut(s) 1262
Ama87I CYCGRG 1 cut(s) 2248
AoxI GGCC 4 cut(s) 193, 705, 806, 1882
ApeKI GCWGC 7 cut(s) 29, 52, 745, 748, 962, 1078, 2083
ApoI RAATTY 5 cut(s) 175, 614, 818, 1803, 2074
Asp700I GAANNNNTTC 3 cut(s) 177, 822, 1002
Asp718I GGTACC 1 cut(s) 445
AspS9I GGNCC 4 cut(s) 193, 706, 790, 1733
AsuC2I CCSGG 2 cut(s) 192, 2124
AsuHPI GGTGA 4 cut(s) 394, 883, 947, 2014
AsuII TTCGAA 1 cut(s) 1769
AvaI CYCGRG 1 cut(s) 2248
AvaII GGWCC 2 cut(s) 790, 1733
AxyI CCTNAGG 1 cut(s) 970
BaeI ACNNNNGTAYC 2 cut(s) 1526, 1559
BalI TGGCCA 1 cut(s) 1884
BanI GGYRCC 1 cut(s) 445
BanII GRGCYC 1 cut(s) 2340
BbsI GAAGAC 5 cut(s) 100, 622, 1709, 2061, 2205
Bbv12I GWGCWC 3 cut(s) 254, 543, 986
BbvCI CCTCAGC 1 cut(s) 2087
BbvI GCAGC 7 cut(s) 16, 64, 732, 760, 974, 1090, 2070
BccI CCATC 4 cut(s) 214, 320, 552, 1379
BcgI CGANNNNNNTGC 2 cut(s) 920, 954
BciT130I CCWGG 3 cut(s) 552, 794, 810
BciVI GTATCC 1 cut(s) 1839
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 2 cut(s) 192, 2124
BcoDI GTCTC 6 cut(s) 969, 1203, 1480, 1503, 2233, 2312
BcuI ACTAGT 1 cut(s) 1759
BfaI CTAG 8 cut(s) 35, 200, 1119, 1709, 1760, 1796, 2069, 2404
BfmI CTRYAG 2 cut(s) 732, 746
BfuAI ACCTGC 1 cut(s) 1815
BfuI GTATCC 1 cut(s) 1839
BisI GCNGC 7 cut(s) 30, 53, 746, 749, 963, 1079, 2084
BlsI GCNGC 7 cut(s) 31, 54, 747, 750, 964, 1080, 2085
BmcAI AGTACT 2 cut(s) 1107, 1713
Bme1390I CCNGG 5 cut(s) 192, 552, 794, 810, 2124
Bme18I GGWCC 2 cut(s) 790, 1733
BmeRI GACNNNNNGTC 1 cut(s) 1312
BmeT110I CYCGRG 1 cut(s) 2248
BmgT120I GGNCC 4 cut(s) 193, 706, 790, 1733
BmiI GGNNCC 6 cut(s) 447, 708, 1467, 1903, 2337, 2399
BmrFI CCNGG 5 cut(s) 192, 552, 794, 810, 2124
BmsI GCATC 5 cut(s) 193, 688, 928, 974, 1168
BoxI GACNNNNGTC 1 cut(s) 375
BpiI GAAGAC 5 cut(s) 100, 622, 1709, 2061, 2205
BpmI CTGGAG 2 cut(s) 116, 573
Bpu10I CCTNAGC 3 cut(s) 1433, 2087, 2093
Bpu14I TTCGAA 1 cut(s) 1769
BpuEI CTTGAG 3 cut(s) 848, 1154, 2126
BpuMI CCSGG 2 cut(s) 192, 2124
BsaBI GATNNNNATC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 702, 1225, 1885
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 191, 899
Bse1I ACTGG 2 cut(s) 133, 1113
Bse21I CCTNAGG 1 cut(s) 970
Bse3DI GCAATG 5 cut(s) 631, 948, 1396, 1966, 2242
Bse8I GATNNNNATC 1 cut(s) 120
BseBI CCWGG 3 cut(s) 552, 794, 810
BseDI CCNNGG 3 cut(s) 702, 1225, 1885
BseGI GGATG 7 cut(s) 262, 331, 563, 703, 802, 965, 1192
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 3 cut(s) 111, 191, 899
BseMI GCAATG 5 cut(s) 631, 948, 1396, 1966, 2242
BseMII CTCAG 7 cut(s) 593, 1269, 1469, 1665, 1731, 2029, 2078
BseNI ACTGG 2 cut(s) 133, 1113
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 7 cut(s) 16, 64, 732, 760, 974, 1090, 2070
BsgI GTGCAG 1 cut(s) 2236
BshFI GGCC 4 cut(s) 195, 707, 808, 1884
BshNI GGYRCC 1 cut(s) 445
BsiHKAI GWGCWC 3 cut(s) 254, 543, 986
BsiHKCI CYCGRG 1 cut(s) 2248
BsiSI CCGG 2 cut(s) 191, 2124
BslI CCNNNNNNNGG 3 cut(s) 111, 191, 899
BsmAI GTCTC 6 cut(s) 969, 1203, 1480, 1503, 2233, 2312
BsmI GAATGC 1 cut(s) 1217
BsnI GGCC 4 cut(s) 195, 707, 808, 1884
BsoBI CYCGRG 1 cut(s) 2248
Bsp119I TTCGAA 1 cut(s) 1769
Bsp1286I GDGCHC 4 cut(s) 254, 543, 986, 2340
Bsp1407I TGTACA 2 cut(s) 673, 2102
Bsp143I GATC 7 cut(s) 115, 121, 329, 520, 812, 1064, 1984
BspACI CCGC 2 cut(s) 435, 1074
BspANI GGCC 4 cut(s) 195, 707, 808, 1884
BspCNI CTCAG 7 cut(s) 592, 1268, 1470, 1666, 1732, 2028, 2079
BspLI GGNNCC 6 cut(s) 447, 708, 1467, 1903, 2337, 2399
BspMAI CTGCAG 1 cut(s) 750
BspMI ACCTGC 1 cut(s) 1815
BspPI GGATC 3 cut(s) 123, 337, 820
BspT104I TTCGAA 1 cut(s) 1769
BspT107I GGYRCC 1 cut(s) 445
BsrDI GCAATG 5 cut(s) 631, 948, 1396, 1966, 2242
BsrGI TGTACA 2 cut(s) 673, 2102
BsrI ACTGG 2 cut(s) 133, 1113
BssECI CCNNGG 3 cut(s) 702, 1225, 1885
BssMI GATC 7 cut(s) 115, 121, 329, 520, 812, 1064, 1984
BssT1I CCWWGG 3 cut(s) 702, 1225, 1885
Bst2UI CCWGG 3 cut(s) 552, 794, 810
Bst6I CTCTTC 1 cut(s) 1581
BstAPI GCANNNNNTGC 2 cut(s) 1429, 1612
BstAUI TGTACA 2 cut(s) 673, 2102
BstBI TTCGAA 1 cut(s) 1769
BstC8I GCNNGC 6 cut(s) 197, 394, 743, 1076, 1561, 1980
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 7 cut(s) 262, 331, 563, 703, 802, 965, 1192
BstKTI GATC 7 cut(s) 118, 124, 332, 523, 815, 1067, 1987
BstMAI GTCTC 6 cut(s) 969, 1203, 1480, 1503, 2233, 2312
BstMBI GATC 7 cut(s) 115, 121, 329, 520, 812, 1064, 1984
BstMWI GCNNNNNNNGC 4 cut(s) 196, 1429, 1612, 2089
BstNI CCWGG 3 cut(s) 552, 794, 810
BstNSI RCATGY 2 cut(s) 1432, 2310
BstPAI GACNNNNGTC 1 cut(s) 375
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 5 cut(s) 190, 550, 792, 808, 2122
BstSFI CTRYAG 2 cut(s) 732, 746
BstV1I GCAGC 7 cut(s) 16, 64, 732, 760, 974, 1090, 2070
BstV2I GAAGAC 5 cut(s) 100, 622, 1709, 2061, 2205
BstX2I RGATCY 2 cut(s) 115, 329
BstXI CCANNNNNNTGG 1 cut(s) 1892
BstYI RGATCY 2 cut(s) 115, 329
Bsu36I CCTNAGG 1 cut(s) 970
BsuI GTATCC 1 cut(s) 1839
BsuRI GGCC 4 cut(s) 195, 707, 808, 1884
BtsCI GGATG 7 cut(s) 262, 331, 563, 703, 802, 965, 1192
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 3 cut(s) 543, 1401, 2023
BveI ACCTGC 1 cut(s) 1815
Cac8I GCNNGC 6 cut(s) 197, 394, 743, 1076, 1561, 1980
CaiI CAGNNNCTG 1 cut(s) 1262
Cfr13I GGNCC 4 cut(s) 193, 706, 790, 1733
CseI GACGC 1 cut(s) 734
Csp6I GTAC 6 cut(s) 446, 674, 1106, 1364, 1712, 2103
CviAII CATG 8 cut(s) 689, 959, 1145, 1424, 1429, 1870, 2236, 2307
CviQI GTAC 6 cut(s) 446, 674, 1106, 1364, 1712, 2103
DpnI GATC 7 cut(s) 117, 123, 331, 522, 814, 1066, 1986
DpnII GATC 7 cut(s) 115, 121, 329, 520, 812, 1064, 1984
DraI TTTAAA 1 cut(s) 210
DrdI GACNNNNNNGTC 1 cut(s) 880
DriI GACNNNNNGTC 1 cut(s) 1312
DseDI GACNNNNNNGTC 1 cut(s) 880
EaeI YGGCCR 1 cut(s) 1882
Eam1104I CTCTTC 1 cut(s) 1581
Eam1105I GACNNNNNGTC 1 cut(s) 1312
EarI CTCTTC 1 cut(s) 1581
Eco130I CCWWGG 3 cut(s) 702, 1225, 1885
Eco24I GRGCYC 1 cut(s) 2340
Eco32I GATATC 1 cut(s) 2383
Eco47I GGWCC 2 cut(s) 790, 1733
Eco57I CTGAAG 8 cut(s) 94, 779, 1260, 1721, 2027, 2073, 2217, 2256
Eco81I CCTNAGG 1 cut(s) 970
Eco88I CYCGRG 1 cut(s) 2248
Eco91I GGTNACC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 706
EcoO65I GGTNACC 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 818
EcoRII CCWGG 3 cut(s) 550, 792, 808
EcoRV GATATC 1 cut(s) 2383
EcoT14I CCWWGG 3 cut(s) 702, 1225, 1885
EcoT38I GRGCYC 1 cut(s) 2340
ErhI CCWWGG 3 cut(s) 702, 1225, 1885
FaeI CATG 8 cut(s) 692, 962, 1148, 1427, 1432, 1873, 2239, 2310
FalI AAGNNNNNCTT 4 cut(s) 644, 676, 871, 903
FatI CATG 8 cut(s) 688, 958, 1144, 1423, 1428, 1869, 2235, 2306
FauI CCCGC 2 cut(s) 428, 1067
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 7 cut(s) 30, 53, 746, 749, 963, 1079, 2084
FokI GGATG 7 cut(s) 249, 338, 570, 710, 809, 952, 1199
FriOI GRGCYC 1 cut(s) 2340
Fsp4HI GCNGC 7 cut(s) 30, 53, 746, 749, 963, 1079, 2084
FspBI CTAG 8 cut(s) 35, 200, 1119, 1709, 1760, 1796, 2069, 2404
GluI GCNGC 7 cut(s) 30, 53, 746, 749, 963, 1079, 2084
GsuI CTGGAG 2 cut(s) 116, 573
HaeIII GGCC 4 cut(s) 195, 707, 808, 1884
HapII CCGG 2 cut(s) 191, 2124
HgaI GACGC 1 cut(s) 734
Hin1II CATG 8 cut(s) 692, 962, 1148, 1427, 1432, 1873, 2239, 2310
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 1171
HinfI GANTC 6 cut(s) 1136, 1251, 1705, 1766, 1788, 2281
HpaII CCGG 2 cut(s) 191, 2124
HphI GGTGA 4 cut(s) 394, 883, 947, 2014
Hpy166II GTNNAC 4 cut(s) 244, 370, 385, 1324
Hpy8I GTNNAC 4 cut(s) 244, 370, 385, 1324
HpyAV CCTTC 5 cut(s) 484, 1824, 2277, 2290, 2350
HpyCH4IV ACGT 2 cut(s) 1024, 1500
HpyCH4V TGCA 6 cut(s) 320, 748, 941, 962, 1959, 2217
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 1429, 1612, 2089
HpySE526I ACGT 2 cut(s) 1024, 1500
Hsp92II CATG 8 cut(s) 692, 962, 1148, 1427, 1432, 1873, 2239, 2310
KpnI GGTACC 1 cut(s) 449
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 7 cut(s) 115, 121, 329, 520, 812, 1064, 1984
LmnI GCTCC 6 cut(s) 135, 257, 553, 981, 2335, 2403
Lsp1109I GCAGC 7 cut(s) 16, 64, 732, 760, 974, 1090, 2070
LweI GCATC 5 cut(s) 193, 688, 928, 974, 1168
MaeI CTAG 8 cut(s) 35, 200, 1119, 1709, 1760, 1796, 2069, 2404
MaeII ACGT 2 cut(s) 1024, 1500
MaeIII GTNAC 9 cut(s) 10, 637, 871, 1414, 1895, 1991, 2020, 2118, 2302
MalI GATC 7 cut(s) 117, 123, 331, 522, 814, 1066, 1986
MboI GATC 7 cut(s) 115, 121, 329, 520, 812, 1064, 1984
MflI RGATCY 2 cut(s) 115, 329
MhlI GDGCHC 4 cut(s) 254, 543, 986, 2340
MlsI TGGCCA 1 cut(s) 1884
MluNI TGGCCA 1 cut(s) 1884
MlyI GAGTC 2 cut(s) 1145, 1699
MmeI TCCRAC 4 cut(s) 286, 451, 1039, 1474
Mox20I TGGCCA 1 cut(s) 1884
MroXI GAANNNNTTC 3 cut(s) 177, 822, 1002
MscI TGGCCA 1 cut(s) 1884
MseI TTAA 9 cut(s) 153, 209, 279, 336, 845, 1356, 1820, 2150, 2286
MslI CAYNNNNRTG 2 cut(s) 1274, 1600
Msp20I TGGCCA 1 cut(s) 1884
MspA1I CMGCKG 1 cut(s) 435
MspI CCGG 2 cut(s) 191, 2124
MspR9I CCNGG 5 cut(s) 192, 552, 794, 810, 2124
Mva1269I GAATGC 1 cut(s) 1217
MvaI CCWGG 3 cut(s) 552, 794, 810
MwoI GCNNNNNNNGC 4 cut(s) 196, 1429, 1612, 2089
NciI CCSGG 2 cut(s) 192, 2124
NdeII GATC 7 cut(s) 115, 121, 329, 520, 812, 1064, 1984
NlaIII CATG 8 cut(s) 692, 962, 1148, 1427, 1432, 1873, 2239, 2310
NlaIV GGNNCC 6 cut(s) 447, 708, 1467, 1903, 2337, 2399
NmuCI GTSAC 4 cut(s) 871, 1991, 2020, 2302
NspI RCATGY 2 cut(s) 1432, 2310
NspV TTCGAA 1 cut(s) 1769
PaeR7I CTCGAG 1 cut(s) 2248
PciI ACATGT 1 cut(s) 2306
PctI GAATGC 1 cut(s) 1217
PdmI GAANNNNTTC 3 cut(s) 177, 822, 1002
PfeI GAWTC 4 cut(s) 1251, 1766, 1788, 2281
PflMI CCANNNNNTGG 1 cut(s) 899
PfoI TCCNGGA 2 cut(s) 550, 792
PkrI GCNGC 7 cut(s) 31, 54, 747, 750, 964, 1080, 2085
PleI GAGTC 2 cut(s) 1144, 1699
PpsI GAGTC 2 cut(s) 1144, 1699
PscI ACATGT 1 cut(s) 2306
PshAI GACNNNNGTC 1 cut(s) 375
Psp1406I AACGTT 1 cut(s) 1024
Psp6I CCWGG 3 cut(s) 550, 792, 808
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 3 cut(s) 550, 792, 808
PspN4I GGNNCC 6 cut(s) 447, 708, 1467, 1903, 2337, 2399
PspPI GGNCC 4 cut(s) 193, 706, 790, 1733
PstI CTGCAG 1 cut(s) 750
PstNI CAGNNNCTG 1 cut(s) 1262
PsuI RGATCY 2 cut(s) 115, 329
RsaI GTAC 6 cut(s) 447, 675, 1107, 1365, 1713, 2104
RsaNI GTAC 6 cut(s) 446, 674, 1106, 1364, 1712, 2103
RseI CAYNNNNRTG 2 cut(s) 1274, 1600
SaqAI TTAA 9 cut(s) 153, 209, 279, 336, 845, 1356, 1820, 2150, 2286
SatI GCNGC 7 cut(s) 30, 53, 746, 749, 963, 1079, 2084
Sau3AI GATC 7 cut(s) 115, 121, 329, 520, 812, 1064, 1984
Sau96I GGNCC 4 cut(s) 193, 706, 790, 1733
ScaI AGTACT 2 cut(s) 1107, 1713
SchI GAGTC 2 cut(s) 1145, 1699
ScrFI CCNGG 5 cut(s) 192, 552, 794, 810, 2124
SduI GDGCHC 4 cut(s) 254, 543, 986, 2340
SfaNI GCATC 5 cut(s) 193, 688, 928, 974, 1168
SfcI CTRYAG 2 cut(s) 732, 746
Sfr274I CTCGAG 1 cut(s) 2248
SfuI TTCGAA 1 cut(s) 1769
SinI GGWCC 2 cut(s) 790, 1733
SlaI CTCGAG 1 cut(s) 2248
SmiMI CAYNNNNRTG 2 cut(s) 1274, 1600
SmlI CTYRAG 4 cut(s) 827, 1133, 2141, 2248
SmoI CTYRAG 4 cut(s) 827, 1133, 2141, 2248
SpeI ACTAGT 1 cut(s) 1759
SsiI CCGC 2 cut(s) 435, 1074
SspI AATATT 2 cut(s) 259, 1948
SspMI CTAG 8 cut(s) 35, 200, 1119, 1709, 1760, 1796, 2069, 2404
StyD4I CCNGG 5 cut(s) 190, 550, 792, 808, 2122
StyI CCWWGG 3 cut(s) 702, 1225, 1885
TaiI ACGT 2 cut(s) 1027, 1503
TaqI TCGA 5 cut(s) 120, 592, 1769, 2129, 2249
TaqII GACCGA 1 cut(s) 1721
TatI WGTACW 4 cut(s) 673, 1105, 1711, 2102
TfiI GAWTC 4 cut(s) 1251, 1766, 1788, 2281
Tru1I TTAA 9 cut(s) 153, 209, 279, 336, 845, 1356, 1820, 2150, 2286
Tru9I TTAA 9 cut(s) 153, 209, 279, 336, 845, 1356, 1820, 2150, 2286
TscAI CASTG 3 cut(s) 550, 1408, 2023
TseFI GTSAC 4 cut(s) 871, 1991, 2020, 2302
TseI GCWGC 7 cut(s) 29, 52, 745, 748, 962, 1078, 2083
Tsp45I GTSAC 4 cut(s) 871, 1991, 2020, 2302
TspGWI ACGGA 2 cut(s) 447, 710
TspRI CASTG 3 cut(s) 550, 1408, 2023
Van91I CCANNNNNTGG 1 cut(s) 899
VpaK11BI GGWCC 2 cut(s) 790, 1733
XapI RAATTY 5 cut(s) 175, 614, 818, 1803, 2074
XbaI TCTAGA 1 cut(s) 1118
XceI RCATGY 2 cut(s) 1432, 2310
XcmI CCANNNNNNNNNTGG 2 cut(s) 22, 1089
XhoI CTCGAG 1 cut(s) 2248
XmiI GTMKAC 1 cut(s) 369
XmnI GAANNNNTTC 3 cut(s) 177, 822, 1002
XspI CTAG 8 cut(s) 35, 200, 1119, 1709, 1760, 1796, 2069, 2404
ZrmI AGTACT 2 cut(s) 1107, 1713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.