Rh2DG176400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000039
Physical Location & Seq
Forward (+)
15493500 .. 15497762
4263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG176400.1

Sequence Viewer

Length: 2457 bp
ATGATGACAGTGGTGATAATCTGCGGAGCTGGTTTAGCGGTGACATTTTGGACGCTGTGTGATTCTGTTAAGCAAGTGAAGGACCAGATACTGATGTTCAAACCCCAACTCGGGGATATCAAGTCCACTCTGGACTCTTTGCAGCCACTGATCAAGGAGATGGCAGAGTGTGAGAAGGAACTGGATGGTCCAGAGGAGGAACTAGAGAATCTTAGAGTGGAAATGCAGGAAGGAGCGGAGCTCATTCGCAAGTGCTCGGAAGCGAGCAGGTTCAAGAAGTATAAGTATGGCAGCAGACTTGTTGAATTGAGCAAGTCACTTCAGAGGCTGTTGGGGATATTGAAAGTGCAGGGCATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGGAACGTTGAGAATGTGGTGCACAGGATTGAAGGGAATTGTGTGATGCAGAACAATCAGTCTGAAAAAATTGAAGGGTGGTGTGCTGTGCCTGAGCCTCCAACAATTACGGTTGGATTGGATTTGCCTTTGAGGGAATTGAAGATGAAGCTCTTTAAGGACGGAGTGTCGATGGTGATACTTACTGCTCCAGGAGGATGCGGGAAAACTACATTGGCAACAAAGTTATGTCAAGATGCAGAAGTCAAAGATAAATTCAAAAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACTTGTACCTAATTGTAAAAGAGCTATATCATCGAACGGGTGCCCAGGTCCCCGATTTCCAAAGTGAAGTAACTGCAGTTAGCTGGCTGCAGAAATTTCTGAAGCAAACAGGTCAAAATCCTATGTTGTTGGTCCTGGATGATGTTTGGGCTGGATCAGAATTCATTCTTGAGAAGTTCGATGAATTAAAAATGACAAATTACAAGATATTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTTTTTGGAATGTTTGAACCGCAAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGTGATAGGAGCTCTTATATTTCTGAAGATCTTCCGAGAAAGATAGTAGAACGTTGTATGGGATATCCACTTGCAATTACAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTGTTGGAATGGTCTAGAGGTTCTTCTGTTCTTAAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGAAGCTTAGATGCTTTGTTTGAAGAGAAGTCTCTTATCAGGGAATGCTTCATAGACCTTGGTTTATTTCCTGAAGATAAAAGAATCTCAGCTACTGTCCTCATTGATATGTGGGCAGAGGTATATGGCATAAATGAGGACATTTCGTCCATTGTGAACCTCTATGAACTTGCCTCTCGGAGTTTGGCTAATCTGGTAGTCACAAGGAAGGACAACAAGGATAGTGATGGCTATTACACTGAACACTTTGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCTGCAGATGAGTCAGGAACCACTGCAACATAGAATGAGGCTCATGATTGACATATGTGGAGACAACCTTCCTATGTGGTGGAAAGAACAAAACTATGAATCCCTCATTGCTCGCCTTGTATCTATCTCGACAGATGAAGAGTTCTCATCTAAATGGCAAAACTTGCAACTACCAGAGGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTGGAGAAAATGAATAAATTGAAGACTCTAGTACTCACAAGTTATGGTATCACTCCGGCTGAGTTGAATAACTTTCAACTAGTTGATTCAAAACCGAATCTGAAGAGAATCAGATTAGAACGAATTTCAATTCCTTCCATAAGCAGGTATCCTATACAGTTGAAAAGTTTGCAAAAGATTTCTCTATTCATGTGTAGAATTGGCCAAGGTTTTGGTAATGGTTCCATCCATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGTTGCCTCCTCAATTGTGTGACCTTGTTCAATTGAAGAAACTCAGTGTCACCAATTCTCATAAACTCTCTGCCTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGCTGTTGAGGCTAAGGTGTTGTACAGACTTGTCAGAGTTACCTGGCTCGGTCAGGAACCTCAAGAAGTTAAACTTGCTTGACATATCTGATTGCTTTAGCATTAAGGAATTGCCTGAAGACATTGGAGAATTGTGCAATTTAGAAAGGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCTTCAGTTTTGGATCTTGAACAGTTGAAGGAAGTGACATGTGATGAAGAGACTGAAATTTTATGGGAACCCTTTTTACCCTTTCTCAAGAACATACACATGAAGGTTGTGAAAGAAGATATCAACCTGAATTGGCTCCACAAGGCACATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

818

Amino Acids

93.49

Weight (kDa)

5.7

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 133 5.4e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 173 - 305 1.4e-15 NB-ARC domain
AAA_22 PF13401 186 - 288 1.7e-06 AAA domain
LRR_14 PF23598 653 - 734 5.1e-09 Leucine-rich repeat region
LRR_14 PF23598 738 - 786 4.8e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 907
Acc36I ACCTGC 2 cut(s) 258, 1848
AccB1I GGYRCC 1 cut(s) 725
AccB7I CCANNNNNTGG 1 cut(s) 926
AccBSI CCGCTC 1 cut(s) 236
AciI CCGC 6 cut(s) 24, 38, 236, 588, 958, 1101
AclI AACGTT 2 cut(s) 393, 1051
AclWI GGATC 2 cut(s) 847, 2322
AcoI YGGCCR 1 cut(s) 1915
AcsI RAATTY 6 cut(s) 641, 779, 845, 1836, 2107, 2358
AcuI CTGAAG 8 cut(s) 305, 806, 1044, 1290, 1835, 2106, 2250, 2289
AfaI GTAC 3 cut(s) 692, 1746, 2137
AfiI CCNNNNNNNGG 5 cut(s) 110, 111, 112, 926, 1857
AflII CTTAAG 1 cut(s) 1163
AflIII ACRYGT 1 cut(s) 2339
AhdI GACNNNNNGTC 2 cut(s) 553, 1342
AhlI ACTAGT 1 cut(s) 1792
AjnI CCWGG 4 cut(s) 577, 729, 819, 2155
AjuI GAANNNNNNNTTGG 6 cut(s) 359, 391, 584, 616, 913, 945
AluBI AGCT 9 cut(s) 29, 241, 538, 709, 768, 1011, 1203, 1289, 2116
AluI AGCT 9 cut(s) 29, 241, 538, 709, 768, 1011, 1203, 1289, 2116
Alw21I GWGCWC 5 cut(s) 243, 257, 411, 1013, 2282
Alw26I GTCTC 4 cut(s) 1233, 1536, 2266, 2345
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 2 cut(s) 847, 2322
AlwNI CAGNNNCTG 4 cut(s) 91, 148, 328, 1292
Ama87I CYCGRG 2 cut(s) 110, 2281
AoxI GGCC 1 cut(s) 1915
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 5 cut(s) 142, 291, 772, 989, 1105
ApoI RAATTY 6 cut(s) 641, 779, 845, 1836, 2107, 2358
ArsI GACNNNNNNTTYG 2 cut(s) 639, 671
Asp700I GAANNNNTTC 2 cut(s) 849, 1029
AspS9I GGNCC 4 cut(s) 82, 188, 733, 817
AsuHPI GGTGA 8 cut(s) 25, 52, 397, 574, 910, 974, 1013, 2047
AvaI CYCGRG 2 cut(s) 110, 2281
AvaII GGWCC 4 cut(s) 82, 188, 733, 817
AxyI CCTNAGG 1 cut(s) 997
BaeGI GKGCMC 2 cut(s) 411, 730
BaeI ACNNNNGTAYC 2 cut(s) 80, 113
BalI TGGCCA 1 cut(s) 1917
BanI GGYRCC 1 cut(s) 725
BanII GRGCYC 2 cut(s) 243, 1013
BbsI GAAGAC 4 cut(s) 649, 1742, 2094, 2238
Bbv12I GWGCWC 5 cut(s) 243, 257, 411, 1013, 2282
BbvI GCAGC 5 cut(s) 154, 303, 759, 1001, 1117
BccI CCATC 5 cut(s) 154, 179, 553, 1418, 1946
BcgI CGANNNNNNTGC 2 cut(s) 363, 397
BciT130I CCWGG 4 cut(s) 579, 731, 821, 2157
BciVI GTATCC 1 cut(s) 1872
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 4 cut(s) 1233, 1536, 2266, 2345
BcuI ACTAGT 1 cut(s) 1792
BfaI CTAG 5 cut(s) 203, 1146, 1742, 1793, 2102
BfmI CTRYAG 3 cut(s) 759, 773, 1484
BfrI CTTAAG 1 cut(s) 1163
BfuAI ACCTGC 2 cut(s) 258, 1848
BfuI GTATCC 1 cut(s) 1872
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 5 cut(s) 143, 292, 773, 990, 1106
BlsI GCNGC 5 cut(s) 144, 293, 774, 991, 1107
BmcAI AGTACT 1 cut(s) 1746
Bme1390I CCNGG 4 cut(s) 579, 731, 821, 2157
Bme18I GGWCC 4 cut(s) 82, 188, 733, 817
BmeRI GACNNNNNGTC 2 cut(s) 553, 1342
BmeT110I CYCGRG 2 cut(s) 110, 2281
BmgT120I GGNCC 4 cut(s) 82, 188, 733, 817
BmiI GGNNCC 7 cut(s) 727, 735, 1500, 1936, 2171, 2370, 2438
BmrFI CCNGG 4 cut(s) 579, 731, 821, 2157
BmsI GCATC 6 cut(s) 423, 575, 613, 955, 1001, 1198
BpiI GAAGAC 4 cut(s) 649, 1742, 2094, 2238
BplI GAGNNNNNCTC 2 cut(s) 225, 257
BpmI CTGGAG 1 cut(s) 561
Bpu10I CCTNAGC 3 cut(s) 480, 1466, 2126
BpuEI CTTGAG 3 cut(s) 875, 2159, 2372
BsaJI CCNNGG 3 cut(s) 729, 1255, 1918
Bsc4I CCNNNNNNNGG 5 cut(s) 110, 111, 112, 926, 1857
Bse1I ACTGG 1 cut(s) 186
Bse21I CCTNAGG 1 cut(s) 997
Bse3DI GCAATG 5 cut(s) 658, 975, 1587, 1999, 2449
BseBI CCWGG 4 cut(s) 579, 731, 821, 2157
BseDI CCNNGG 3 cut(s) 729, 1255, 1918
BseGI GGATG 6 cut(s) 190, 367, 590, 829, 992, 1938
BseLI CCNNNNNNNGG 5 cut(s) 110, 111, 112, 926, 1857
BseMI GCAATG 5 cut(s) 658, 975, 1587, 1999, 2449
BseMII CTCAG 5 cut(s) 471, 1299, 1698, 1764, 2062
BseNI ACTGG 1 cut(s) 186
BseRI GAGGAG 2 cut(s) 209, 2004
BseSI GKGCMC 2 cut(s) 411, 730
BseXI GCAGC 5 cut(s) 154, 303, 759, 1001, 1117
BsgI GTGCAG 1 cut(s) 368
BshFI GGCC 1 cut(s) 1917
BshNI GGYRCC 1 cut(s) 725
BsiHKAI GWGCWC 5 cut(s) 243, 257, 411, 1013, 2282
BsiHKCI CYCGRG 2 cut(s) 110, 2281
BsiSI CCGG 1 cut(s) 1769
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 5 cut(s) 110, 111, 112, 926, 1857
BsmAI GTCTC 4 cut(s) 1233, 1536, 2266, 2345
BsmFI GGGAC 1 cut(s) 719
BsmI GAATGC 1 cut(s) 1247
BsnI GGCC 1 cut(s) 1917
BsoBI CYCGRG 2 cut(s) 110, 2281
Bsp1286I GDGCHC 6 cut(s) 243, 257, 411, 730, 1013, 2282
Bsp1407I TGTACA 1 cut(s) 2135
Bsp143I GATC 5 cut(s) 150, 839, 1027, 1091, 2314
BspACI CCGC 6 cut(s) 24, 38, 236, 588, 958, 1101
BspANI GGCC 1 cut(s) 1917
BspCNI CTCAG 5 cut(s) 472, 1298, 1699, 1765, 2061
BspHI TCATGA 1 cut(s) 1524
BspLI GGNNCC 7 cut(s) 727, 735, 1500, 1936, 2171, 2370, 2438
BspMAI CTGCAG 3 cut(s) 763, 777, 1488
BspMI ACCTGC 2 cut(s) 258, 1848
BspPI GGATC 2 cut(s) 847, 2322
BspT107I GGYRCC 1 cut(s) 725
BspTI CTTAAG 1 cut(s) 1163
BsrBI CCGCTC 1 cut(s) 236
BsrDI GCAATG 5 cut(s) 658, 975, 1587, 1999, 2449
BsrGI TGTACA 1 cut(s) 2135
BsrI ACTGG 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 729, 1255, 1918
BssMI GATC 5 cut(s) 150, 839, 1027, 1091, 2314
BssT1I CCWWGG 2 cut(s) 1255, 1918
Bst2UI CCWGG 4 cut(s) 579, 731, 821, 2157
Bst4CI ACNGT 8 cut(s) 10, 499, 670, 1081, 1294, 1872, 2277, 2325
Bst6I CTCTTC 4 cut(s) 1215, 1614, 1811, 2343
BstAFI CTTAAG 1 cut(s) 1163
BstAPI GCANNNNNTGC 2 cut(s) 1462, 1645
BstAUI TGTACA 1 cut(s) 2135
BstC8I GCNNGC 4 cut(s) 265, 770, 1103, 1594
BstF5I GGATG 6 cut(s) 190, 367, 590, 829, 992, 1938
BstKTI GATC 5 cut(s) 153, 842, 1030, 1094, 2317
BstMAI GTCTC 4 cut(s) 1233, 1536, 2266, 2345
BstMBI GATC 5 cut(s) 150, 839, 1027, 1091, 2314
BstMWI GCNNNNNNNGC 4 cut(s) 35, 1462, 1645, 2122
BstNI CCWGG 4 cut(s) 579, 731, 821, 2157
BstNSI RCATGY 2 cut(s) 1465, 2343
BstSCI CCNGG 4 cut(s) 577, 729, 819, 2155
BstSFI CTRYAG 3 cut(s) 759, 773, 1484
BstSLI GKGCMC 2 cut(s) 411, 730
BstV1I GCAGC 5 cut(s) 154, 303, 759, 1001, 1117
BstV2I GAAGAC 4 cut(s) 649, 1742, 2094, 2238
BstX2I RGATCY 2 cut(s) 1027, 2314
BstXI CCANNNNNNTGG 1 cut(s) 1925
BstYI RGATCY 2 cut(s) 1027, 2314
Bsu36I CCTNAGG 1 cut(s) 997
BsuI GTATCC 1 cut(s) 1872
BsuRI GGCC 1 cut(s) 1917
BtsCI GGATG 6 cut(s) 190, 367, 590, 829, 992, 1938
BtsI GCAGTG 1 cut(s) 1502
BtsIMutI CAGTG 6 cut(s) 15, 146, 1434, 1502, 2056, 2282
BveI ACCTGC 2 cut(s) 258, 1848
Cac8I GCNNGC 4 cut(s) 265, 770, 1103, 1594
CaiI CAGNNNCTG 4 cut(s) 91, 148, 328, 1292
CciI TCATGA 1 cut(s) 1524
Cfr13I GGNCC 4 cut(s) 82, 188, 733, 817
CseI GACGC 1 cut(s) 61
Csp6I GTAC 3 cut(s) 691, 1745, 2136
CviAII CATG 9 cut(s) 986, 1175, 1457, 1462, 1525, 1903, 2269, 2340, 2401
CviQI GTAC 3 cut(s) 691, 1745, 2136
DpnI GATC 5 cut(s) 152, 841, 1029, 1093, 2316
DpnII GATC 5 cut(s) 150, 839, 1027, 1091, 2314
DrdI GACNNNNNNGTC 1 cut(s) 907
DriI GACNNNNNGTC 2 cut(s) 553, 1342
DseDI GACNNNNNNGTC 1 cut(s) 907
EaeI YGGCCR 1 cut(s) 1915
Eam1104I CTCTTC 4 cut(s) 1215, 1614, 1811, 2343
Eam1105I GACNNNNNGTC 2 cut(s) 553, 1342
EarI CTCTTC 4 cut(s) 1215, 1614, 1811, 2343
Ecl136II GAGCTC 2 cut(s) 241, 1011
Eco130I CCWWGG 2 cut(s) 1255, 1918
Eco24I GRGCYC 2 cut(s) 243, 1013
Eco32I GATATC 3 cut(s) 118, 1064, 2422
Eco47I GGWCC 4 cut(s) 82, 188, 733, 817
Eco53kI GAGCTC 2 cut(s) 241, 1011
Eco57I CTGAAG 8 cut(s) 305, 806, 1044, 1290, 1835, 2106, 2250, 2289
Eco81I CCTNAGG 1 cut(s) 997
Eco88I CYCGRG 2 cut(s) 110, 2281
EcoICRI GAGCTC 2 cut(s) 241, 1011
EcoO109I RGGNCCY 1 cut(s) 733
EcoRI GAATTC 1 cut(s) 845
EcoRII CCWGG 4 cut(s) 577, 729, 819, 2155
EcoRV GATATC 3 cut(s) 118, 1064, 2422
EcoT14I CCWWGG 2 cut(s) 1255, 1918
EcoT38I GRGCYC 2 cut(s) 243, 1013
ErhI CCWWGG 2 cut(s) 1255, 1918
FaeI CATG 9 cut(s) 989, 1178, 1460, 1465, 1528, 1906, 2272, 2343, 2404
FalI AAGNNNNNCTT 6 cut(s) 671, 703, 898, 930, 2171, 2203
FaqI GGGAC 1 cut(s) 719
FatI CATG 9 cut(s) 985, 1174, 1456, 1461, 1524, 1902, 2268, 2339, 2400
FauI CCCGC 2 cut(s) 581, 1094
FauNDI CATATG 1 cut(s) 1535
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 5 cut(s) 143, 292, 773, 990, 1106
FokI GGATG 6 cut(s) 197, 374, 597, 836, 979, 1925
FriOI GRGCYC 2 cut(s) 243, 1013
Fsp4HI GCNGC 5 cut(s) 143, 292, 773, 990, 1106
FspBI CTAG 5 cut(s) 203, 1146, 1742, 1793, 2102
GluI GCNGC 5 cut(s) 143, 292, 773, 990, 1106
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 1 cut(s) 1917
HapII CCGG 1 cut(s) 1769
HgaI GACGC 1 cut(s) 61
Hin1II CATG 9 cut(s) 989, 1178, 1460, 1465, 1528, 1906, 2272, 2343, 2404
HindIII AAGCTT 1 cut(s) 1201
HpaII CCGG 1 cut(s) 1769
HphI GGTGA 8 cut(s) 25, 52, 397, 574, 910, 974, 1013, 2047
Hpy166II GTNNAC 3 cut(s) 126, 409, 1354
Hpy8I GTNNAC 3 cut(s) 126, 409, 1354
HpyCH4III ACNGT 8 cut(s) 10, 499, 670, 1081, 1294, 1872, 2277, 2325
HpyCH4IV ACGT 2 cut(s) 393, 1051
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 1462, 1645, 2122
HpySE526I ACGT 2 cut(s) 393, 1051
Hsp92II CATG 9 cut(s) 989, 1178, 1460, 1465, 1528, 1906, 2272, 2343, 2404
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 5 cut(s) 150, 839, 1027, 1091, 2314
LmnI GCTCC 6 cut(s) 26, 233, 238, 580, 1008, 2442
Lsp1109I GCAGC 5 cut(s) 154, 303, 759, 1001, 1117
LweI GCATC 6 cut(s) 423, 575, 613, 955, 1001, 1198
MaeI CTAG 5 cut(s) 203, 1146, 1742, 1793, 2102
MaeII ACGT 2 cut(s) 393, 1051
MalI GATC 5 cut(s) 152, 841, 1029, 1093, 2316
MbiI CCGCTC 1 cut(s) 236
MboI GATC 5 cut(s) 150, 839, 1027, 1091, 2314
MfeI CAATTG 2 cut(s) 2018, 2036
MflI RGATCY 2 cut(s) 1027, 2314
MhlI GDGCHC 6 cut(s) 243, 257, 411, 730, 1013, 2282
MlsI TGGCCA 1 cut(s) 1917
MluNI TGGCCA 1 cut(s) 1917
MlyI GAGTC 3 cut(s) 128, 1501, 1732
MmeI TCCRAC 4 cut(s) 481, 512, 1066, 1116
Mox20I TGGCCA 1 cut(s) 1917
MroXI GAANNNNTTC 2 cut(s) 849, 1029
MscI TGGCCA 1 cut(s) 1917
MseI TTAA 6 cut(s) 69, 543, 872, 1164, 2183, 2217
MslI CAYNNNNRTG 3 cut(s) 1304, 1633, 2399
Msp20I TGGCCA 1 cut(s) 1917
MspCI CTTAAG 1 cut(s) 1163
MspI CCGG 1 cut(s) 1769
MspR9I CCNGG 4 cut(s) 579, 731, 821, 2157
MunI CAATTG 2 cut(s) 2018, 2036
Mva1269I GAATGC 1 cut(s) 1247
MvaI CCWGG 4 cut(s) 579, 731, 821, 2157
MwoI GCNNNNNNNGC 4 cut(s) 35, 1462, 1645, 2122
NdeI CATATG 1 cut(s) 1535
NdeII GATC 5 cut(s) 150, 839, 1027, 1091, 2314
NlaIII CATG 9 cut(s) 989, 1178, 1460, 1465, 1528, 1906, 2272, 2343, 2404
NlaIV GGNNCC 7 cut(s) 727, 735, 1500, 1936, 2171, 2370, 2438
NmuCI GTSAC 7 cut(s) 40, 315, 898, 1396, 2024, 2053, 2335
NspI RCATGY 2 cut(s) 1465, 2343
PaeR7I CTCGAG 1 cut(s) 2281
PagI TCATGA 1 cut(s) 1524
PciI ACATGT 1 cut(s) 2339
PctI GAATGC 1 cut(s) 1247
PdmI GAANNNNTTC 2 cut(s) 849, 1029
PfeI GAWTC 7 cut(s) 62, 208, 1281, 1580, 1799, 1810, 1821
PflMI CCANNNNNTGG 1 cut(s) 926
PfoI TCCNGGA 2 cut(s) 577, 819
PkrI GCNGC 5 cut(s) 144, 293, 774, 991, 1107
PleI GAGTC 3 cut(s) 128, 1500, 1732
PpsI GAGTC 3 cut(s) 128, 1500, 1732
PpuMI RGGWCCY 1 cut(s) 733
PscI ACATGT 1 cut(s) 2339
Psp124BI GAGCTC 2 cut(s) 243, 1013
Psp1406I AACGTT 2 cut(s) 393, 1051
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 4 cut(s) 577, 729, 819, 2155
PspGI CCWGG 4 cut(s) 577, 729, 819, 2155
PspN4I GGNNCC 7 cut(s) 727, 735, 1500, 1936, 2171, 2370, 2438
PspPI GGNCC 4 cut(s) 82, 188, 733, 817
PspPPI RGGWCCY 1 cut(s) 733
PsrI GAACNNNNNNTAC 2 cut(s) 2361, 2393
PstI CTGCAG 3 cut(s) 763, 777, 1488
PstNI CAGNNNCTG 4 cut(s) 91, 148, 328, 1292
PsuI RGATCY 2 cut(s) 1027, 2314
RsaI GTAC 3 cut(s) 692, 1746, 2137
RsaNI GTAC 3 cut(s) 691, 1745, 2136
RseI CAYNNNNRTG 3 cut(s) 1304, 1633, 2399
SacI GAGCTC 2 cut(s) 243, 1013
SaqAI TTAA 6 cut(s) 69, 543, 872, 1164, 2183, 2217
SatI GCNGC 5 cut(s) 143, 292, 773, 990, 1106
Sau3AI GATC 5 cut(s) 150, 839, 1027, 1091, 2314
Sau96I GGNCC 4 cut(s) 82, 188, 733, 817
ScaI AGTACT 1 cut(s) 1746
SchI GAGTC 3 cut(s) 128, 1501, 1732
ScrFI CCNGG 4 cut(s) 579, 731, 821, 2157
SduI GDGCHC 6 cut(s) 243, 257, 411, 730, 1013, 2282
SfaNI GCATC 6 cut(s) 423, 575, 613, 955, 1001, 1198
SfcI CTRYAG 3 cut(s) 759, 773, 1484
Sfr274I CTCGAG 1 cut(s) 2281
SinI GGWCC 4 cut(s) 82, 188, 733, 817
SlaI CTCGAG 1 cut(s) 2281
SmiMI CAYNNNNRTG 3 cut(s) 1304, 1633, 2399
SmlI CTYRAG 5 cut(s) 854, 1163, 2174, 2281, 2387
SmoI CTYRAG 5 cut(s) 854, 1163, 2174, 2281, 2387
SpeI ACTAGT 1 cut(s) 1792
SsiI CCGC 6 cut(s) 24, 38, 236, 588, 958, 1101
SspI AATATT 1 cut(s) 1981
SspMI CTAG 5 cut(s) 203, 1146, 1742, 1793, 2102
SstI GAGCTC 2 cut(s) 243, 1013
StyD4I CCNGG 4 cut(s) 577, 729, 819, 2155
StyI CCWWGG 2 cut(s) 1255, 1918
TaaI ACNGT 8 cut(s) 10, 499, 670, 1081, 1294, 1872, 2277, 2325
TaiI ACGT 2 cut(s) 396, 1054
TaqI TCGA 5 cut(s) 557, 718, 864, 1610, 2282
TaqII GACCGA 1 cut(s) 2152
TatI WGTACW 2 cut(s) 1744, 2135
TfiI GAWTC 7 cut(s) 62, 208, 1281, 1580, 1799, 1810, 1821
Tru1I TTAA 6 cut(s) 69, 543, 872, 1164, 2183, 2217
Tru9I TTAA 6 cut(s) 69, 543, 872, 1164, 2183, 2217
TscAI CASTG 6 cut(s) 15, 153, 1441, 1509, 2056, 2282
TseFI GTSAC 7 cut(s) 40, 315, 898, 1396, 2024, 2053, 2335
TseI GCWGC 5 cut(s) 142, 291, 772, 989, 1105
Tsp45I GTSAC 7 cut(s) 40, 315, 898, 1396, 2024, 2053, 2335
TspGWI ACGGA 1 cut(s) 564
TspRI CASTG 6 cut(s) 15, 153, 1441, 1509, 2056, 2282
Van91I CCANNNNNTGG 1 cut(s) 926
Vha464I CTTAAG 1 cut(s) 1163
VneI GTGCAC 1 cut(s) 407
VpaK11BI GGWCC 4 cut(s) 82, 188, 733, 817
XapI RAATTY 6 cut(s) 641, 779, 845, 1836, 2107, 2358
XbaI TCTAGA 1 cut(s) 1145
XceI RCATGY 2 cut(s) 1465, 2343
XcmI CCANNNNNNNNNTGG 1 cut(s) 1116
XhoI CTCGAG 1 cut(s) 2281
XmnI GAANNNNTTC 2 cut(s) 849, 1029
XspI CTAG 5 cut(s) 203, 1146, 1742, 1793, 2102
ZrmI AGTACT 1 cut(s) 1746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.