FvH4_1g15230

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000039
Physical Location & Seq
Forward (+)
8645057 .. 8654897
9841 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15230.t1

Sequence Viewer

Length: 3993 bp
ATGGCATCATTGAAGGAGTTTACTGCAAATTTTGTGAAACTTGATCGCTTTGACGGAGCAAATTTCAGACGTTGGCAAAAGAAGTTGCATTTCCTACTATCAAGCTTAAAGGTCGTCTACGTGCTTACTACTCCAAAACCTGTAGAAACAGAAGATGACACCCTTGCTGCACAACGTGAAAGACTGAAGTGGGAACAAGATGATTATGTCTGCAAGGGCCATATATGCAATTCCATGTCTGATGCCTTATTTGATATCTACCATGAGATGGCTACTGCAAAGGAAATATGGGACGCATTGGAGGCTAAGTATATCTCACATGATGCTACAAGTAAGAAATTTCTGGTCCAAAAGTTCTTCAATTATAAGATGACTGATGATAGATCAATTGTTGAACAATTCCATGAGATTATGCATATACACAATCAGTTTAATCAATTCAAAATGCATATGGATGATTCTATAATTGTTTCTGCAATTATGGATAAACTTCCACCATCTTGGAAGGAATGCAAGAAGAGCCTCAAACATAAGAAAGAGGACATGTCCATAGAGCAGTTGGGGACCCATCTTCGCATAGAAGAAGAATGCAGATCCCGAGAGAAAACTGATGATTCTGATTTCTCTTCCAAAGTTCATATGGTTGATGAAGGGCCTAAACATTCAAAAATCAGCCATGGAAATAATAAGAGAAAGGGCAACTTTTCCAAGAAAGGTTTCCAAAGTCAGAATGTAAAGAAGACAAAAGGAGGATGCTTCTTTTGTGGCAAGCCTGGTCACTACAAGAAAGATTGTCGTCATCTCAAGAACAAACAAGGAACTACCAATGAAAAGTATGTGGCAATGATTTCTGAAATCAATATGATGGAGGATATTGAAGCTTGGTGGATAGACTCTGGTGCTACAAAGCATGTCTGCAAGAACAAAGATATGTTCAAAACATATGAACCAAGTGGAGATGACAACGTACTCTACATGGGAAACTCTTCTACTTCTTCTATCAAAGGAAAAGGGACAATTGAATTGGAGTTCACATCAGGAAAAGTTCTTACTCTTACTGATGTCTACCATGTTCCAGATGTTAGAAAGAATTTGGTTTCTGCTAGTCTTTTGAACAAGTCTGGATTCAAGCTTGTCATGGAATCTAACAAGTTTATTCTGACCAAGGGTGGTACCTTTGTAGGAAAAGGGTATATGTATGATGGAATGTTCAAACTCAATATTAATAAAGTATCTACTATTTCTGTTTACATGCTTGATTCTTTATCTTTATGGCATAATAGATTAGGACTAGATAATATGGCAAATCTGGGTTTAATCCCAAAGCCTGTGAATGATATGCATGATAAATGTGTTACTTGCTTACAAACAAAACTAACTAGAAAGCCTTTTCCAAATAAGCCAAGAAACAATGATAGTGAATCATTGTTATCTGGGAGAACTAGACTCCATAGTGAGTCTGATGAACCAACTGAAATTCGAAGGAGCAAAAGAAGAAGGATAGAAAAATCCTTTGGACCAGATTTTGTTGTGTACCTTGTAGAAGTGGTATTCATCGGAGGAGCGCGTTTGTCAGTGACATTTTGGACGCTGTGTGATTCTCTCAAGCAAGTGAAGGATATGATGGTGTTCAAATCCCAACTCGGGGATATCGAATCCACTCTGCACTCTTTGGAACCACTGATCAAGGAGATGGCAGAGTGTGAGAAGGAGTTGGATGGTCAAGGGGAGGAGCTAGAGAATCTTAGTGTGGAAATGGAAGAAGGAGTGGAGCTTATTCGCAAGTGCTCGAAAGCGAGCAGGTTCAAGAAGTATAAGTATGGCAGCCGGCTTGTTGAATTGGGTAAGTCACTTCAGAGGCTGTTGGGGATACTGAAAGTGCAGGGCATAAGGGATGTTAAGAAGGCATTGATTTCGATGAGGAATATTGAGAATGTGGTTCAGAGAATTGAAGGGAATTGTGTGATACAGAACATTCAGTCTGAGAAAATTGAAGGGTGGTGTGCTGTGCCTGAACCTACGACTGTTACGGTTGGATTGGATGTGCCTCTGAGGGAACTGAAAATGAAGCTCTTTACTTATGGAGTTTCGATGGTGATAGTTACTGCTCCCGGAGGATGTGGGAAAACTACCCTGGCTACAAAGTTGTGCCAAGATGCAGAAATCAAAGATAAATTCAAGAGCAATATCTTCTTTGTTACTGTCACAAAGAAGCACAACTTATTTCTGATTGTCCAAGAGCTATATCATCGAACGGGTGCCCAGGTCCCCGATTTCCAAAGTGATGTAACTGCAGTTAGCTGGATGCACCAATTTCTGAAGAAAACTGGACAAAATCCTATGTTGTTGGTCCTGGATGATGTCTGGGCTGGATCAGAATTTATTCTGGAGAAGTTTGATGAATTAAAAATGACAAATTGCAAGATATTGGTTACATCAAGGTCTAGTTTTCCAAGATTTGGTTCTCCATATTATCTAGAATGTTTGAACGATGCAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGAGACAGGAGCTCTTATATATCAGAAGATCTTCCGAGAAAGATAGTTGAGCGTTGTATGGGATTTCCACTAGCAATTACAGTTCTCGGAAGATCACTATGCGGTCAGCCAATAGAGATATGGCAAAAAAGAGTATTGGAATGGTCTAGAGGTTCATCTGTTCTTGAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGTAGCTTAGATGCGTTGGATGAAGAGAAGCCTCTTATCAGGGAATGCTTCATCGACCTTGGTTTATTTCCCGAAGATCAAAGAATCCCTGCTACTGTCCTAGTTGATATGTGGGCAGAGTTATATGGCATACATGAAGATATTTTGTCAATTTCACACCTCTATGAACTTGCCTCTCAAAGTTTGGCTAATCTGGTGGTCACAAGCAGGAAGGACAACAAGTACAGTGATGGCTATTACACTGAACACTTCGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCGACAGACGAGTCAGGAACCGCTACAACAGAGAATGAGACTTATGATTGACATATGTGGAGACAACCTTCCTATGTGGTGGAAAGAACAAAAGTATCAATCCCTCATCGCTCGTGTAGTATCTATCTCGACAGATGAAGAGTTCTCATCTACATGGCAAAACTTGCAACTACCAGAAGCAGAGGTTTTGATTTTGAATTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTAGAGAAAATGAAAAAACTGAAGACTCTAGTACTCACAAGTTGCAGTAGTAGTCCTACCGAGTTGAATAACTTTCAACTAGTTGATTCGATACCAAGTTTAAAGAGAATCAGATTGGAGCGAATATCACTTCCTTCCATAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTTGCATCGGCCAAGGTTTTGGTAACGGTACCATACAGATATCAGAGGCGTTTCCAAATGTTGTTGAAATGAATATTGACTACTGCAATGATTTGGTTGAGTTGCCTGCCCAACTCTGTGACCTTATACAACTCAAGAAGCTCAGTGTTACCAATTCTCATAAGCTCTCTGCCTTACCTGAAGACATTGGAAATCTAGTCAATTTAGAGCTGCTGAGGCTTAGGTCTTGTACAGACTTGTCAGAGTTACCTGGCTCTATTAGGAACCTCAAGAAGTTGAACTTTCTTGACATATCGGATTGCTTTAGCATGAAGGAATTGCCTGAGAACATTGGAGAATTGTGCAGCTTAGAAAGGCTCAACATGAGACAGTGCTCGAGATTGAATGAGCTGCCTTCTTCAGTTTTGGATCTTGAACACTTGAAGGAAGTGACATGCGATGAAGAGACAGAAATTTTATGGGAACCCTTCTTACCCTATCTGACAAACATGCACATTAAGGTTGTGAAAGAAGATATCAACCTCAATTGGCTCCACAAGTCTCATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

1331

Amino Acids

152.35

Weight (kDa)

6.77

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 33 - 202 4.7e-29 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 295 - 376 1.4e-26 Pol polyprotein, beta-barrel domain
RPW8 PF05659 519 - 644 1.8e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 684 - 816 1.4e-14 NB-ARC domain
LRR_14 PF23598 1184 - 1248 2.5e-09 Leucine-rich repeat region
LRR_14 PF23598 1250 - 1299 3.3e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
AasI GACNNNNNNGTC 1 cut(s) 3027
Acc36I ACCTGC 2 cut(s) 1791, 3384
Acc65I GGTACC 2 cut(s) 1172, 3470
AccB1I GGYRCC 3 cut(s) 1172, 2258, 3470
AccB7I CCANNNNNTGG 2 cut(s) 268, 2459
AccI GTMKAC 2 cut(s) 117, 1065
AccII CGCG 1 cut(s) 1567
AciI CCGC 2 cut(s) 2634, 3038
AclWI GGATC 3 cut(s) 588, 2380, 3858
AcoI YGGCCR 1 cut(s) 3451
AcsI RAATTY 9 cut(s) 28, 61, 338, 1090, 1476, 2174, 2378, 3214, 3894
AcuI CTGAAG 6 cut(s) 206, 1838, 2339, 3290, 3642, 3825
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 7 cut(s) 969, 1174, 1535, 2954, 3282, 3472, 3673
AfiI CCNNNNNNNGG 5 cut(s) 268, 1644, 1645, 2459, 3393
AflIII ACRYGT 1 cut(s) 543
AhlI ACTAGT 1 cut(s) 3328
AjnI CCWGG 5 cut(s) 772, 2133, 2262, 2352, 3691
AjuI GAANNNNNNNTTGG 6 cut(s) 701, 733, 1497, 1529, 2446, 2478
Alw21I GWGCWC 3 cut(s) 1790, 2546, 3818
Alw26I GTCTC 6 cut(s) 2529, 3049, 3072, 3802, 3881, 3987
AlwI GGATC 3 cut(s) 588, 2380, 3858
AlwNI CAGNNNCTG 1 cut(s) 1861
Ama87I CYCGRG 3 cut(s) 597, 1643, 3817
AoxI GGCC 3 cut(s) 217, 653, 3451
ApeKI GCWGC 6 cut(s) 167, 1824, 2522, 3652, 3786, 3832
ApoI RAATTY 9 cut(s) 28, 61, 338, 1090, 1476, 2174, 2378, 3214, 3894
AseI ATTAAT 1 cut(s) 1224
Asp700I GAANNNNTTC 4 cut(s) 716, 985, 2382, 2562
Asp718I GGTACC 2 cut(s) 1172, 3470
AspLEI GCGC 1 cut(s) 1567
AspS9I GGNCC 7 cut(s) 217, 346, 564, 653, 1517, 2266, 2350
AsuC2I CCSGG 1 cut(s) 2112
AsuHPI GGTGA 2 cut(s) 2107, 2507
AsuII TTCGAA 1 cut(s) 1480
AvaI CYCGRG 3 cut(s) 597, 1643, 3817
AvaII GGWCC 5 cut(s) 346, 564, 1517, 2266, 2350
AxyI CCTNAGG 1 cut(s) 2530
BaeGI GKGCMC 1 cut(s) 2263
BaeI ACNNNNGTAYC 4 cut(s) 1164, 1197, 3095, 3128
BanI GGYRCC 3 cut(s) 1172, 2258, 3470
BanII GRGCYC 1 cut(s) 2546
BauI CACGAG 1 cut(s) 3129
BbsI GAAGAC 3 cut(s) 746, 3278, 3630
Bbv12I GWGCWC 3 cut(s) 1790, 2546, 3818
BbvCI CCTCAGC 1 cut(s) 3656
BbvI GCAGC 6 cut(s) 154, 1836, 2534, 3639, 3798, 3819
BcgI CGANNNNNNTGC 4 cut(s) 1896, 1930, 2480, 2514
BciT130I CCWGG 5 cut(s) 774, 2135, 2264, 2354, 3693
BciVI GTATCC 2 cut(s) 1863, 3408
BclI TGATCA 1 cut(s) 1683
BcnI CCSGG 1 cut(s) 2112
BcoDI GTCTC 6 cut(s) 2529, 3049, 3072, 3802, 3881, 3987
BcuI ACTAGT 1 cut(s) 3328
BfmI CTRYAG 2 cut(s) 141, 2292
BfuAI ACCTGC 2 cut(s) 1791, 3384
BfuI GTATCC 2 cut(s) 1863, 3408
BglII AGATCT 1 cut(s) 2560
BisI GCNGC 6 cut(s) 168, 1825, 2523, 3653, 3787, 3833
BlsI GCNGC 6 cut(s) 169, 1826, 2524, 3654, 3788, 3834
BmcAI AGTACT 1 cut(s) 3282
Bme1390I CCNGG 6 cut(s) 774, 2112, 2135, 2264, 2354, 3693
Bme18I GGWCC 5 cut(s) 346, 564, 1517, 2266, 2350
BmeT110I CYCGRG 3 cut(s) 597, 1643, 3817
BmgT120I GGNCC 7 cut(s) 217, 346, 564, 653, 1517, 2266, 2350
BmrFI CCNGG 6 cut(s) 774, 2112, 2135, 2264, 2354, 3693
BpiI GAAGAC 3 cut(s) 746, 3278, 3630
BpmI CTGGAG 1 cut(s) 2408
Bpu10I CCTNAGC 3 cut(s) 3002, 3656, 3662
Bpu14I TTCGAA 1 cut(s) 1480
BpuEI CTTGAG 5 cut(s) 788, 1589, 2717, 3560, 3695
BpuMI CCSGG 1 cut(s) 2112
BsaAI YACGTR 1 cut(s) 121
BsaJI CCNNGG 6 cut(s) 676, 1164, 2133, 2262, 2788, 3454
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 5 cut(s) 268, 1644, 1645, 2459, 3393
Bse118I RCCGGY 1 cut(s) 1827
Bse1I ACTGG 1 cut(s) 2332
Bse21I CCTNAGG 1 cut(s) 2530
Bse3DI GCAATG 3 cut(s) 849, 2508, 3535
BseBI CCWGG 5 cut(s) 774, 2135, 2264, 2354, 3693
BseDI CCNNGG 6 cut(s) 676, 1164, 2133, 2262, 2788, 3454
BseLI CCNNNNNNNGG 5 cut(s) 268, 1644, 1645, 2459, 3393
BseMI GCAATG 3 cut(s) 849, 2508, 3535
BseMII CTCAG 6 cut(s) 1974, 2042, 3234, 3598, 3647, 3756
BseNI ACTGG 1 cut(s) 2332
BseRI GAGGAG 2 cut(s) 1575, 1745
BseSI GKGCMC 1 cut(s) 2263
BseXI GCAGC 6 cut(s) 154, 1836, 2534, 3639, 3798, 3819
BsgI GTGCAG 4 cut(s) 153, 1649, 1901, 3805
Bsh1236I CGCG 1 cut(s) 1567
BshFI GGCC 3 cut(s) 219, 655, 3453
BshNI GGYRCC 3 cut(s) 1172, 2258, 3470
BsiHKAI GWGCWC 3 cut(s) 1790, 2546, 3818
BsiHKCI CYCGRG 3 cut(s) 597, 1643, 3817
BsiSI CCGG 2 cut(s) 1828, 2112
BslFI GGGAC 4 cut(s) 305, 577, 1027, 2252
BslI CCNNNNNNNGG 5 cut(s) 268, 1644, 1645, 2459, 3393
BsmAI GTCTC 6 cut(s) 2529, 3049, 3072, 3802, 3881, 3987
BsmFI GGGAC 4 cut(s) 305, 577, 1027, 2252
BsmI GAATGC 3 cut(s) 515, 593, 2780
BsnI GGCC 3 cut(s) 219, 655, 3453
BsoBI CYCGRG 3 cut(s) 597, 1643, 3817
Bsp119I TTCGAA 1 cut(s) 1480
Bsp1286I GDGCHC 4 cut(s) 1790, 2263, 2546, 3818
Bsp1407I TGTACA 1 cut(s) 3671
Bsp143I GATC 9 cut(s) 43, 383, 593, 1683, 2372, 2560, 2624, 2806, 3850
Bsp19I CCATGG 1 cut(s) 676
BspACI CCGC 2 cut(s) 2634, 3038
BspANI GGCC 3 cut(s) 219, 655, 3453
BspCNI CTCAG 6 cut(s) 1975, 2043, 3235, 3597, 3648, 3757
BspFNI CGCG 1 cut(s) 1567
BspMAI CTGCAG 1 cut(s) 2296
BspMI ACCTGC 2 cut(s) 1791, 3384
BspPI GGATC 3 cut(s) 588, 2380, 3858
BspQI GCTCTTC 1 cut(s) 512
BspT104I TTCGAA 1 cut(s) 1480
BspT107I GGYRCC 3 cut(s) 1172, 2258, 3470
BsrDI GCAATG 3 cut(s) 849, 2508, 3535
BsrFI RCCGGY 1 cut(s) 1827
BsrGI TGTACA 1 cut(s) 3671
BsrI ACTGG 1 cut(s) 2332
BssAI RCCGGY 1 cut(s) 1827
BssECI CCNNGG 6 cut(s) 676, 1164, 2133, 2262, 2788, 3454
BssMI GATC 9 cut(s) 43, 383, 593, 1683, 2372, 2560, 2624, 2806, 3850
BssSI CACGAG 1 cut(s) 3129
BssT1I CCWWGG 4 cut(s) 676, 1164, 2788, 3454
Bst2BI CACGAG 1 cut(s) 3129
Bst2UI CCWGG 5 cut(s) 774, 2135, 2264, 2354, 3693
Bst4CI ACNGT 9 cut(s) 2026, 2032, 2203, 2614, 2827, 2957, 3408, 3470, 3813
Bst6I CTCTTC 6 cut(s) 512, 631, 991, 2748, 3150, 3879
BstAPI GCANNNNNTGC 2 cut(s) 2998, 3181
BstAUI TGTACA 1 cut(s) 3671
BstBAI YACGTR 1 cut(s) 121
BstBI TTCGAA 1 cut(s) 1480
BstC8I GCNNGC 4 cut(s) 770, 1798, 1829, 3549
BstDSI CCRYGG 1 cut(s) 676
BstFNI CGCG 1 cut(s) 1567
BstHHI GCGC 1 cut(s) 1567
BstKTI GATC 9 cut(s) 46, 386, 596, 1686, 2375, 2563, 2627, 2809, 3853
BstMAI GTCTC 6 cut(s) 2529, 3049, 3072, 3802, 3881, 3987
BstMBI GATC 9 cut(s) 43, 383, 593, 1683, 2372, 2560, 2624, 2806, 3850
BstMWI GCNNNNNNNGC 6 cut(s) 225, 302, 519, 2998, 3181, 3658
BstNI CCWGG 5 cut(s) 774, 2135, 2264, 2354, 3693
BstNSI RCATGY 6 cut(s) 547, 914, 1255, 3001, 3879, 3934
BstSCI CCNGG 6 cut(s) 772, 2110, 2133, 2262, 2352, 3691
BstSFI CTRYAG 2 cut(s) 141, 2292
BstSLI GKGCMC 1 cut(s) 2263
BstUI CGCG 1 cut(s) 1567
BstV1I GCAGC 6 cut(s) 154, 1836, 2534, 3639, 3798, 3819
BstV2I GAAGAC 3 cut(s) 746, 3278, 3630
BstX2I RGATCY 3 cut(s) 593, 2560, 3850
BstXI CCANNNNNNTGG 2 cut(s) 501, 3461
BstYI RGATCY 3 cut(s) 593, 2560, 3850
Bsu36I CCTNAGG 1 cut(s) 2530
BsuI GTATCC 2 cut(s) 1863, 3408
BsuRI GGCC 3 cut(s) 219, 655, 3453
BtgI CCRYGG 1 cut(s) 676
BtgZI GCGATG 2 cut(s) 3109, 3894
BtsIMutI CAGTG 6 cut(s) 1581, 1679, 2962, 2970, 3592, 3818
BveI ACCTGC 2 cut(s) 1791, 3384
Cac8I GCNNGC 4 cut(s) 770, 1798, 1829, 3549
CaiI CAGNNNCTG 1 cut(s) 1861
CfoI GCGC 1 cut(s) 1567
Cfr10I RCCGGY 1 cut(s) 1827
Cfr13I GGNCC 7 cut(s) 217, 346, 564, 653, 1517, 2266, 2350
CseI GACGC 2 cut(s) 302, 1597
Csp6I GTAC 7 cut(s) 968, 1173, 1534, 2953, 3281, 3471, 3672
CviQI GTAC 7 cut(s) 968, 1173, 1534, 2953, 3281, 3471, 3672
DpnI GATC 9 cut(s) 45, 385, 595, 1685, 2374, 2562, 2626, 2808, 3852
DpnII GATC 9 cut(s) 43, 383, 593, 1683, 2372, 2560, 2624, 2806, 3850
DraI TTTAAA 1 cut(s) 3351
DraIII CACNNNGTG 1 cut(s) 176
DrdI GACNNNNNNGTC 1 cut(s) 3027
DseDI GACNNNNNNGTC 1 cut(s) 3027
EaeI YGGCCR 1 cut(s) 3451
Eam1104I CTCTTC 6 cut(s) 512, 631, 991, 2748, 3150, 3879
EarI CTCTTC 6 cut(s) 512, 631, 991, 2748, 3150, 3879
Ecl136II GAGCTC 1 cut(s) 2544
Eco130I CCWWGG 4 cut(s) 676, 1164, 2788, 3454
Eco24I GRGCYC 1 cut(s) 2546
Eco32I GATATC 4 cut(s) 256, 1651, 3483, 3958
Eco47I GGWCC 5 cut(s) 346, 564, 1517, 2266, 2350
Eco53kI GAGCTC 1 cut(s) 2544
Eco57I CTGAAG 6 cut(s) 206, 1838, 2339, 3290, 3642, 3825
Eco81I CCTNAGG 1 cut(s) 2530
Eco88I CYCGRG 3 cut(s) 597, 1643, 3817
EcoICRI GAGCTC 1 cut(s) 2544
EcoO109I RGGNCCY 3 cut(s) 564, 653, 2266
EcoRII CCWGG 5 cut(s) 772, 2133, 2262, 2352, 3691
EcoRV GATATC 4 cut(s) 256, 1651, 3483, 3958
EcoT14I CCWWGG 4 cut(s) 676, 1164, 2788, 3454
EcoT22I ATGCAT 3 cut(s) 417, 450, 1344
EcoT38I GRGCYC 1 cut(s) 2546
ErhI CCWWGG 4 cut(s) 676, 1164, 2788, 3454
FalI AAGNNNNNCTT 6 cut(s) 686, 718, 2204, 2236, 3707, 3739
FaqI GGGAC 4 cut(s) 305, 577, 1027, 2252
FauNDI CATATG 4 cut(s) 450, 639, 943, 3071
FbaI TGATCA 1 cut(s) 1683
FblI GTMKAC 2 cut(s) 117, 1065
Fnu4HI GCNGC 6 cut(s) 168, 1825, 2523, 3653, 3787, 3833
FriOI GRGCYC 1 cut(s) 2546
Fsp4HI GCNGC 6 cut(s) 168, 1825, 2523, 3653, 3787, 3833
GlaI GCGC 1 cut(s) 1566
GluI GCNGC 6 cut(s) 168, 1825, 2523, 3653, 3787, 3833
GsuI CTGGAG 1 cut(s) 2408
HaeIII GGCC 3 cut(s) 219, 655, 3453
HapII CCGG 2 cut(s) 1828, 2112
HgaI GACGC 2 cut(s) 302, 1597
HhaI GCGC 1 cut(s) 1567
Hin6I GCGC 1 cut(s) 1565
HinP1I GCGC 1 cut(s) 1565
HindIII AAGCTT 3 cut(s) 103, 879, 1130
HpaII CCGG 2 cut(s) 1828, 2112
HphI GGTGA 2 cut(s) 2107, 2507
Hpy166II GTNNAC 6 cut(s) 21, 118, 1032, 1066, 1249, 1534
Hpy8I GTNNAC 6 cut(s) 21, 118, 1032, 1066, 1249, 1534
HpyCH4III ACNGT 9 cut(s) 2026, 2032, 2203, 2614, 2827, 2957, 3408, 3470, 3813
HpyCH4IV ACGT 4 cut(s) 70, 120, 175, 966
HpyF10VI GCNNNNNNNGC 6 cut(s) 225, 302, 519, 2998, 3181, 3658
HpySE526I ACGT 4 cut(s) 70, 120, 175, 966
HspAI GCGC 1 cut(s) 1565
KflI GGGWCCC 1 cut(s) 564
KpnI GGTACC 2 cut(s) 1176, 3474
KroI GCCGGC 1 cut(s) 1827
KroNI GCCGGC 1 cut(s) 1829
Ksp22I TGATCA 1 cut(s) 1683
Kzo9I GATC 9 cut(s) 43, 383, 593, 1683, 2372, 2560, 2624, 2806, 3850
LguI GCTCTTC 1 cut(s) 512
LmnI GCTCC 9 cut(s) 56, 1485, 1562, 1732, 1771, 2113, 2541, 3367, 3978
Lsp1109I GCAGC 6 cut(s) 154, 1836, 2534, 3639, 3798, 3819
MaeII ACGT 4 cut(s) 70, 120, 175, 966
MalI GATC 9 cut(s) 45, 385, 595, 1685, 2374, 2562, 2626, 2808, 3852
MboI GATC 9 cut(s) 43, 383, 593, 1683, 2372, 2560, 2624, 2806, 3850
MfeI CAATTG 3 cut(s) 387, 1017, 3967
MflI RGATCY 3 cut(s) 593, 2560, 3850
MhlI GDGCHC 4 cut(s) 1790, 2263, 2546, 3818
MlyI GAGTC 6 cut(s) 887, 1440, 1466, 2708, 3037, 3268
MmeI TCCRAC 4 cut(s) 1695, 2014, 2727, 3043
Mph1103I ATGCAT 3 cut(s) 417, 450, 1344
MroNI GCCGGC 1 cut(s) 1827
MroXI GAANNNNTTC 4 cut(s) 716, 985, 2382, 2562
MseI TTAA 8 cut(s) 107, 432, 1224, 1316, 1899, 2405, 3350, 3939
MslI CAYNNNNRTG 3 cut(s) 453, 2892, 3169
MspI CCGG 2 cut(s) 1828, 2112
MspR9I CCNGG 6 cut(s) 774, 2112, 2135, 2264, 2354, 3693
MunI CAATTG 3 cut(s) 387, 1017, 3967
Mva1269I GAATGC 3 cut(s) 515, 593, 2780
MvaI CCWGG 5 cut(s) 774, 2135, 2264, 2354, 3693
MvnI CGCG 1 cut(s) 1567
MwoI GCNNNNNNNGC 6 cut(s) 225, 302, 519, 2998, 3181, 3658
NaeI GCCGGC 1 cut(s) 1829
NciI CCSGG 1 cut(s) 2112
NcoI CCATGG 1 cut(s) 676
NdeI CATATG 4 cut(s) 450, 639, 943, 3071
NdeII GATC 9 cut(s) 43, 383, 593, 1683, 2372, 2560, 2624, 2806, 3850
NgoMIV GCCGGC 1 cut(s) 1827
NmuCI GTSAC 7 cut(s) 776, 1576, 1848, 2203, 2929, 3560, 3871
NsiI ATGCAT 3 cut(s) 417, 450, 1344
NspI RCATGY 6 cut(s) 547, 914, 1255, 3001, 3879, 3934
NspV TTCGAA 1 cut(s) 1480
PaeR7I CTCGAG 1 cut(s) 3817
PciI ACATGT 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 512
PcsI WCGNNNNNNNCGW 1 cut(s) 1650
PctI GAATGC 3 cut(s) 515, 593, 2780
PdiI GCCGGC 1 cut(s) 1829
PdmI GAANNNNTTC 4 cut(s) 716, 985, 2382, 2562
PflMI CCANNNNNTGG 2 cut(s) 268, 2459
PfoI TCCNGGA 2 cut(s) 2110, 2352
PkrI GCNGC 6 cut(s) 169, 1826, 2524, 3654, 3788, 3834
PleI GAGTC 6 cut(s) 887, 1440, 1465, 2707, 3036, 3268
PpsI GAGTC 6 cut(s) 887, 1440, 1465, 2707, 3036, 3268
Ppu21I YACGTR 1 cut(s) 121
PpuMI RGGWCCY 2 cut(s) 564, 2266
PscI ACATGT 1 cut(s) 543
PshBI ATTAAT 1 cut(s) 1224
PsiI TTATAA 1 cut(s) 366
Psp124BI GAGCTC 1 cut(s) 2546
Psp5II RGGWCCY 2 cut(s) 564, 2266
Psp6I CCWGG 5 cut(s) 772, 2133, 2262, 2352, 3691
PspGI CCWGG 5 cut(s) 772, 2133, 2262, 2352, 3691
PspPI GGNCC 7 cut(s) 217, 346, 564, 653, 1517, 2266, 2350
PspPPI RGGWCCY 2 cut(s) 564, 2266
PsrI GAACNNNNNNTAC 2 cut(s) 3897, 3929
PstI CTGCAG 1 cut(s) 2296
PstNI CAGNNNCTG 1 cut(s) 1861
PsuI RGATCY 3 cut(s) 593, 2560, 3850
RsaI GTAC 7 cut(s) 969, 1174, 1535, 2954, 3282, 3472, 3673
RsaNI GTAC 7 cut(s) 968, 1173, 1534, 2953, 3281, 3471, 3672
RseI CAYNNNNRTG 3 cut(s) 453, 2892, 3169
SacI GAGCTC 1 cut(s) 2546
SapI GCTCTTC 1 cut(s) 512
SaqAI TTAA 8 cut(s) 107, 432, 1224, 1316, 1899, 2405, 3350, 3939
SatI GCNGC 6 cut(s) 168, 1825, 2523, 3653, 3787, 3833
Sau3AI GATC 9 cut(s) 43, 383, 593, 1683, 2372, 2560, 2624, 2806, 3850
Sau96I GGNCC 7 cut(s) 217, 346, 564, 653, 1517, 2266, 2350
ScaI AGTACT 1 cut(s) 3282
SchI GAGTC 6 cut(s) 887, 1440, 1466, 2708, 3037, 3268
ScrFI CCNGG 6 cut(s) 774, 2112, 2135, 2264, 2354, 3693
SduI GDGCHC 4 cut(s) 1790, 2263, 2546, 3818
SfcI CTRYAG 2 cut(s) 141, 2292
Sfr274I CTCGAG 1 cut(s) 3817
SfuI TTCGAA 1 cut(s) 1480
SinI GGWCC 5 cut(s) 346, 564, 1517, 2266, 2350
SlaI CTCGAG 1 cut(s) 3817
SmiMI CAYNNNNRTG 3 cut(s) 453, 2892, 3169
SmlI CTYRAG 6 cut(s) 803, 1604, 2696, 3575, 3710, 3817
SmoI CTYRAG 6 cut(s) 803, 1604, 2696, 3575, 3710, 3817
SpeI ACTAGT 1 cut(s) 3328
SsiI CCGC 2 cut(s) 2634, 3038
SspI AATATT 3 cut(s) 1222, 1927, 3517
SstI GAGCTC 1 cut(s) 2546
StyD4I CCNGG 6 cut(s) 772, 2110, 2133, 2262, 2352, 3691
StyI CCWWGG 4 cut(s) 676, 1164, 2788, 3454
TaaI ACNGT 9 cut(s) 2026, 2032, 2203, 2614, 2827, 2957, 3408, 3470, 3813
TaiI ACGT 4 cut(s) 73, 123, 178, 969
TatI WGTACW 3 cut(s) 2952, 3280, 3671
Tru1I TTAA 8 cut(s) 107, 432, 1224, 1316, 1899, 2405, 3350, 3939
Tru9I TTAA 8 cut(s) 107, 432, 1224, 1316, 1899, 2405, 3350, 3939
TscAI CASTG 6 cut(s) 1581, 1686, 2962, 2977, 3592, 3818
TseFI GTSAC 7 cut(s) 776, 1576, 1848, 2203, 2929, 3560, 3871
TseI GCWGC 6 cut(s) 167, 1824, 2522, 3652, 3786, 3832
Tsp45I GTSAC 7 cut(s) 776, 1576, 1848, 2203, 2929, 3560, 3871
TspGWI ACGGA 1 cut(s) 69
TspRI CASTG 6 cut(s) 1581, 1686, 2962, 2977, 3592, 3818
Van91I CCANNNNNTGG 2 cut(s) 268, 2459
VpaK11BI GGWCC 5 cut(s) 346, 564, 1517, 2266, 2350
VspI ATTAAT 1 cut(s) 1224
XapI RAATTY 9 cut(s) 28, 61, 338, 1090, 1476, 2174, 2378, 3214, 3894
XbaI TCTAGA 2 cut(s) 2476, 2678
XceI RCATGY 6 cut(s) 547, 914, 1255, 3001, 3879, 3934
XcmI CCANNNNNNNNNTGG 3 cut(s) 556, 637, 2649
XhoI CTCGAG 1 cut(s) 3817
XmiI GTMKAC 2 cut(s) 117, 1065
XmnI GAANNNNTTC 4 cut(s) 716, 985, 2382, 2562
ZrmI AGTACT 1 cut(s) 3282
Zsp2I ATGCAT 3 cut(s) 417, 450, 1344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.