RchiOBHm_Chr2g0104381

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000039
Physical Location & Seq
Forward (+)
15748231 .. 15755762
7532 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47865

Sequence Viewer

Length: 2373 bp
ATGTTTAAAGCCCTCCTTGGAGATATCAGATCTACACTAGATTCTATAGAACCACTGATCAAAGAGACAGCAGAGGATGACAAGGAGTTGGATTTTCCAGAGGATGAACTTCAGAATTTTGGAGTACAAATGGAAGAGGGGATTGAGCTAATTCGTAAGTGCTCTAAGGTTGGTCCATTGAGTACTTACAAAAAATATAAATACACAAACAAACTAGGTGAACTGGGAAAATCTTTGCAAAGACTGTTGGATATACTGAAAGTGCATAGCATAAGAGATGTGAAGAAAACCTTGGTTACCATAAAGAATATTGAGACTAAGGTCCAGCAAATTGATGTGAATATTGGGATACAAAATAATTCATCTGAAAGTATTGAAGGTTCATGTGCAATACCTGAACCTCCACCACTTGTAGTTGGATTGGATGGGCCTTTGAAGGAATTGAAGATGAAGCTTTTCAGTGATGAGATATCAATGCTTGTGCTAACTGCTCCTGGAGGATGTGGGAAAACTACATTGGCAACGAAGTTTTGTCAAGATGGAGACGTCAGAGATAAATTCAAGAACAATATCTTCTTTGTAACTGTTTCCAAGAAGCCCAACTTAAACCTTATTGTAGAAGAGCTATATCAACGCAAGGGTTCCCAGGTACCCGATTTCCAACATGAAGTAATTGCACTTAATTGGCTGTACGAATTTCTAAACCAAATGGGACAAGATCCTTTACTATTGGTTTTGGATGATGTTTGGTCTGAATCTGAATTTCTTCTTGAGAAGTTTGATGAATGGAGAATGTCTAACTGCAAGATCTTGGTGACATCAAGGTTTGCATTTCCAAGATTTGGTTCTCCATATAATTTGAAATCATTGAGTGATGAAGATGCATTTGCTCTTTTTAATCATTCAGCATACTTGGGAGATGTTCGCTCTCATGTTCCAGAAGACCTTGCAAGACAGGTAATAAAACATTGTAAAGGATTTCCTCTTGCAATTACAGTGGTTGGAAGATCACTTTGTGGGCAGCCTATAGAGATCTGGCAAAAAAGATTAATTGAATGGACTAAAGGTTCATCTATTCTTGATTCTGATCATGATTTGCTTGTTTGCCTCCAAAGCAGCCTTGATGCCTTGGAGAAACAAAGTATTACCATCAAGGAATGTTTCCTAGACCTTGGTTCATTTCCAGAAGACCAAAGAATACCTGCTACTACCCTCATTGATATATGGGCGGAGTCATATAAAATAGATGAAGACTGGCTTTGCATTGCAAACCTCTGTGAACTCACCAATAGAAATCTGGCTACTCTTATAATCACAAGGAAGGACCCCAGGGAGGTGGATGGCTATTACAGTGAACATTTTGTTACTCTGCATGACATTATTAGAGAATTGGCTATCTATCAGTCAAGTGTGGAATCAATAGAACAGAGAAAAAGGCTGATAATAGATATGTCTGGAGACAATTTTCCCGAGTGGTGGACGGAACAGAAATGTCAACCCATCAATGCTCACCTTTTATCTATCTCGACAGATGGAGACTTCTCATCTAAATGGCTCAACATGCAACTACCACAAGCAGAGGTTCTAATTCTGAACTTTCAGTCAAAGAAGTATGCCTTACCTGAATTTGTGGAGAAAATGGACAAATTAAAGGTTCTGATAGTCACAAAATACGGTCTTTTACCTGCTGAATTGGATAACTTTCCATTACTTGGTTCATTATCAAATCTAAAGAGAATTAGATTAGAACGAATAGCAATACCTTCCATGAGCAAGACCCCTGTACAGTTGAAGAGTTTAGAAAAGATATCATTATTCATGTGCAAAGTGGGTCAAGCCTTTGACAACTGTTCCATCGAACTTTTTAATCTATTGCCAAATGTAGTGGAAATGAACATTGATTACTGCAATGATTTGGTGAAATTGCCCGCCATGCTTTGTGACCTGATTTGCCTGAAAAAGCTTAGTATCACCAATTCTCACAAGCTATCTGTCTTACCTGAAGAAATAGGAAAGTTAGTGAACTTAAAAGTGTTGAGGCTTAGGTCTTGTACTGAATTGTCAGAGCTGCCAGACTCGATTCGGAACCTCAAATTGTTAAATTTTCTGGACATATCTGATTGCTTTAGTATGAAGGAGCTTCCTGAACATATTGGTGAAATGTGCTGCTTAGAAACGCTAAATATGAGACAATGCTCAAGACTGCAAGAGTTGCCTGCATCAGTTTTGGATCTTGAACAGTTGAAGGATGTGATTTGTGATGAAGAGACTGAAATATTATGGAACCCCTTCTTACCCATTCTCCACAACTTACATATCAGGGTAGTGAAAGAAGATATTAACCTGAATTGGCTCCACACGTTTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

790

Amino Acids

90.26

Weight (kDa)

5.17

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 1 - 109 1.4e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 144 - 303 6.2e-16 NB-ARC domain
LRR_14 PF23598 645 - 707 5.1e-08 Leucine-rich repeat region
LRR_14 PF23598 694 - 759 1.5e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1310
AatII GACGTC 1 cut(s) 549
Acc36I ACCTGC 2 cut(s) 1210, 1693
Acc65I GGTACC 1 cut(s) 649
AccB1I GGYRCC 1 cut(s) 649
AccB7I CCANNNNNTGG 2 cut(s) 842, 1712
AciI CCGC 2 cut(s) 1229, 1929
AclWI GGATC 2 cut(s) 713, 2238
AcsI RAATTY 6 cut(s) 115, 557, 695, 761, 1625, 2101
AcuI CTGAAG 2 cut(s) 95, 2022
AcyI GRCGYC 1 cut(s) 546
AdeI CACNNNGTG 1 cut(s) 1016
AfaI GTAC 6 cut(s) 126, 184, 651, 692, 1785, 2053
AfiI CCNNNNNNNGG 4 cut(s) 842, 1333, 1476, 1712
AflIII ACRYGT 1 cut(s) 2358
AgsI TTSAA 9 cut(s) 377, 436, 445, 562, 862, 1055, 1792, 2237, 2245
AjnI CCWGG 3 cut(s) 493, 645, 1328
AjuI GAANNNNNNNTTGG 6 cut(s) 275, 307, 500, 532, 829, 861
AloI GAACNNNNNNTCC 2 cut(s) 1051, 1083
AluBI AGCT 7 cut(s) 148, 454, 625, 1963, 1987, 2068, 2140
AluI AGCT 7 cut(s) 148, 454, 625, 1963, 1987, 2068, 2140
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 7 cut(s) 59, 308, 537, 1452, 1530, 2182, 2261
AlwI GGATC 2 cut(s) 713, 2238
Ama87I CYCGRG 1 cut(s) 1469
AoxI GGCC 1 cut(s) 428
ApeKI GCWGC 4 cut(s) 1021, 1116, 2068, 2166
ApoI RAATTY 6 cut(s) 115, 557, 695, 761, 1625, 2101
AseI ATTAAT 1 cut(s) 1049
Asp700I GAANNNNTTC 3 cut(s) 455, 765, 2288
Asp718I GGTACC 1 cut(s) 649
AspS9I GGNCC 4 cut(s) 173, 322, 428, 1324
AsuHPI GGTGA 7 cut(s) 230, 826, 1276, 1502, 1930, 1963, 2168
AvaI CYCGRG 1 cut(s) 1469
AvaII GGWCC 3 cut(s) 173, 322, 1324
BanI GGYRCC 1 cut(s) 649
BbsI GAAGAC 3 cut(s) 948, 1194, 1257
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 4 cut(s) 1033, 1128, 2055, 2153
BccI CCATC 7 cut(s) 419, 533, 1157, 1334, 1508, 1526, 1862
BciT130I CCWGG 3 cut(s) 495, 647, 1330
BciVI GTATCC 1 cut(s) 342
BclI TGATCA 2 cut(s) 57, 1087
BcoDI GTCTC 7 cut(s) 59, 308, 537, 1452, 1530, 2182, 2261
BfaI CTAG 3 cut(s) 38, 215, 1166
BfmI CTRYAG 2 cut(s) 45, 1026
BfuAI ACCTGC 2 cut(s) 1210, 1693
BfuI GTATCC 1 cut(s) 342
BglII AGATCT 3 cut(s) 29, 807, 1032
BisI GCNGC 4 cut(s) 1022, 1117, 2069, 2167
BlsI GCNGC 4 cut(s) 1023, 1118, 2070, 2168
BmcAI AGTACT 1 cut(s) 184
Bme1390I CCNGG 3 cut(s) 495, 647, 1330
Bme18I GGWCC 3 cut(s) 173, 322, 1324
BmeT110I CYCGRG 1 cut(s) 1469
BmgT120I GGNCC 4 cut(s) 173, 322, 428, 1324
BmiI GGNNCC 6 cut(s) 643, 651, 1326, 2087, 2285, 2354
BmrFI CCNGG 3 cut(s) 495, 647, 1330
BmrI ACTGGG 1 cut(s) 233
BmsI GCATC 3 cut(s) 871, 1114, 2228
BmuI ACTGGG 1 cut(s) 233
BoxI GACNNNNGTC 1 cut(s) 320
BpiI GAAGAC 3 cut(s) 948, 1194, 1257
BpmI CTGGAG 2 cut(s) 516, 1476
Bpu10I CCTNAGC 1 cut(s) 2042
BpuEI CTTGAG 2 cut(s) 791, 2182
BsaBI GATNNNNATC 1 cut(s) 1086
BsaHI GRCGYC 1 cut(s) 546
BsaJI CCNNGG 7 cut(s) 16, 291, 645, 1128, 1171, 1328, 1329
BsaXI ACNNNNNCTCC 2 cut(s) 2286, 2316
Bsc4I CCNNNNNNNGG 4 cut(s) 842, 1333, 1476, 1712
Bse1I ACTGG 2 cut(s) 228, 1259
Bse3DI GCAATG 2 cut(s) 1263, 1915
Bse8I GATNNNNATC 1 cut(s) 1086
BseBI CCWGG 3 cut(s) 495, 647, 1330
BseDI CCNNGG 7 cut(s) 16, 291, 645, 1128, 1171, 1328, 1329
BseGI GGATG 7 cut(s) 82, 109, 430, 506, 745, 1345, 2254
BseJI GATNNNNATC 1 cut(s) 1086
BseLI CCNNNNNNNGG 4 cut(s) 842, 1333, 1476, 1712
BseMI GCAATG 2 cut(s) 1263, 1915
BseNI ACTGG 2 cut(s) 228, 1259
BseXI GCAGC 4 cut(s) 1033, 1128, 2055, 2153
BshFI GGCC 1 cut(s) 430
BshNI GGYRCC 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 164
BsiHKCI CYCGRG 1 cut(s) 1469
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 4 cut(s) 842, 1333, 1476, 1712
BsmAI GTCTC 7 cut(s) 59, 308, 537, 1452, 1530, 2182, 2261
BsmBI CGTCTC 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 726
BsnI GGCC 1 cut(s) 430
BsoBI CYCGRG 1 cut(s) 1469
Bsp1286I GDGCHC 1 cut(s) 164
Bsp1407I TGTACA 1 cut(s) 1783
Bsp143I GATC 8 cut(s) 29, 57, 718, 807, 1007, 1032, 1087, 2230
BspACI CCGC 2 cut(s) 1229, 1929
BspANI GGCC 1 cut(s) 430
BspHI TCATGA 1 cut(s) 1090
BspLI GGNNCC 6 cut(s) 643, 651, 1326, 2087, 2285, 2354
BspMI ACCTGC 2 cut(s) 1210, 1693
BspPI GGATC 2 cut(s) 713, 2238
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 1 cut(s) 649
BsrDI GCAATG 2 cut(s) 1263, 1915
BsrGI TGTACA 1 cut(s) 1783
BsrI ACTGG 2 cut(s) 228, 1259
BssECI CCNNGG 7 cut(s) 16, 291, 645, 1128, 1171, 1328, 1329
BssMI GATC 8 cut(s) 29, 57, 718, 807, 1007, 1032, 1087, 2230
BssNI GRCGYC 1 cut(s) 546
BssT1I CCWWGG 4 cut(s) 16, 291, 1128, 1171
Bst2UI CCWGG 3 cut(s) 495, 647, 1330
Bst4CI ACNGT 8 cut(s) 246, 586, 997, 1352, 1676, 1788, 1850, 2241
Bst6I CTCTTC 4 cut(s) 129, 615, 1787, 2259
BstACI GRCGYC 1 cut(s) 546
BstAPI GCANNNNNTGC 1 cut(s) 2212
BstAUI TGTACA 1 cut(s) 1783
BstC8I GCNNGC 2 cut(s) 1929, 2217
BstDEI CTNAG 5 cut(s) 165, 318, 1964, 2042, 2170
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 7 cut(s) 82, 109, 430, 506, 745, 1345, 2254
BstKTI GATC 8 cut(s) 32, 60, 721, 810, 1010, 1035, 1090, 2233
BstMAI GTCTC 7 cut(s) 59, 308, 537, 1452, 1530, 2182, 2261
BstMBI GATC 8 cut(s) 29, 57, 718, 807, 1007, 1032, 1087, 2230
BstMWI GCNNNNNNNGC 4 cut(s) 1113, 1561, 1933, 2212
BstNI CCWGG 3 cut(s) 495, 647, 1330
BstNSI RCATGY 1 cut(s) 1564
BstPAI GACNNNNGTC 1 cut(s) 320
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 3 cut(s) 493, 645, 1328
BstSFI CTRYAG 2 cut(s) 45, 1026
BstV1I GCAGC 4 cut(s) 1033, 1128, 2055, 2153
BstV2I GAAGAC 3 cut(s) 948, 1194, 1257
BstX2I RGATCY 5 cut(s) 29, 718, 807, 1032, 2230
BstXI CCANNNNNNTGG 1 cut(s) 1336
BstYI RGATCY 5 cut(s) 29, 718, 807, 1032, 2230
BsuI GTATCC 1 cut(s) 342
BsuRI GGCC 1 cut(s) 430
BtsCI GGATG 7 cut(s) 82, 109, 430, 506, 745, 1345, 2254
BtsIMutI CAGTG 4 cut(s) 53, 466, 1002, 1357
BveI ACCTGC 2 cut(s) 1210, 1693
Cac8I GCNNGC 2 cut(s) 1929, 2217
CciI TCATGA 1 cut(s) 1090
Cfr13I GGNCC 4 cut(s) 173, 322, 428, 1324
Csp6I GTAC 6 cut(s) 125, 183, 650, 691, 1784, 2052
CspCI CAANNNNNGTGG 4 cut(s) 978, 1013, 1866, 1901
CviAII CATG 9 cut(s) 384, 665, 932, 1091, 1373, 1561, 1768, 1819, 1933
CviQI GTAC 6 cut(s) 125, 183, 650, 691, 1784, 2052
DdeI CTNAG 5 cut(s) 165, 318, 1964, 2042, 2170
DpnI GATC 8 cut(s) 31, 59, 720, 809, 1009, 1034, 1089, 2232
DpnII GATC 8 cut(s) 29, 57, 718, 807, 1007, 1032, 1087, 2230
DraI TTTAAA 1 cut(s) 7
DraIII CACNNNGTG 1 cut(s) 1016
Eam1104I CTCTTC 4 cut(s) 129, 615, 1787, 2259
EarI CTCTTC 4 cut(s) 129, 615, 1787, 2259
EciI GGCGGA 1 cut(s) 1244
Eco130I CCWWGG 4 cut(s) 16, 291, 1128, 1171
Eco32I GATATC 3 cut(s) 25, 471, 1809
Eco47I GGWCC 3 cut(s) 173, 322, 1324
Eco57I CTGAAG 2 cut(s) 95, 2022
Eco88I CYCGRG 1 cut(s) 1469
Eco91I GGTNACC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 1324
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 3 cut(s) 493, 645, 1328
EcoRV GATATC 3 cut(s) 25, 471, 1809
EcoT14I CCWWGG 4 cut(s) 16, 291, 1128, 1171
EcoT22I ATGCAT 1 cut(s) 886
ErhI CCWWGG 4 cut(s) 16, 291, 1128, 1171
Esp3I CGTCTC 1 cut(s) 537
FaeI CATG 9 cut(s) 387, 668, 935, 1094, 1376, 1564, 1771, 1822, 1936
FalI AAGNNNNNCTT 9 cut(s) 32, 275, 307, 587, 619, 1242, 1274, 1601, 1633
FaqI GGGAC 1 cut(s) 726
FatI CATG 9 cut(s) 383, 664, 931, 1090, 1372, 1560, 1767, 1818, 1932
FauI CCCGC 1 cut(s) 1936
FbaI TGATCA 2 cut(s) 57, 1087
Fnu4HI GCNGC 4 cut(s) 1022, 1117, 2069, 2167
FokI GGATG 7 cut(s) 89, 116, 437, 513, 752, 1352, 2261
Fsp4HI GCNGC 4 cut(s) 1022, 1117, 2069, 2167
FspBI CTAG 3 cut(s) 38, 215, 1166
GluI GCNGC 4 cut(s) 1022, 1117, 2069, 2167
GsuI CTGGAG 2 cut(s) 516, 1476
HaeIII GGCC 1 cut(s) 430
Hin1I GRCGYC 1 cut(s) 546
Hin1II CATG 9 cut(s) 387, 668, 935, 1094, 1376, 1564, 1771, 1822, 1936
HincII GTYRAC 1 cut(s) 1496
HindII GTYRAC 1 cut(s) 1496
HindIII AAGCTT 2 cut(s) 452, 1961
HinfI GANTC 7 cut(s) 41, 755, 1082, 1232, 1415, 2075, 2080
HphI GGTGA 7 cut(s) 230, 826, 1276, 1502, 1930, 1963, 2168
Hpy166II GTNNAC 6 cut(s) 221, 1280, 1355, 1479, 1496, 2023
Hpy8I GTNNAC 6 cut(s) 221, 1280, 1355, 1479, 1496, 2023
HpyAV CCTTC 7 cut(s) 371, 430, 1315, 1773, 2128, 2239, 2299
HpyCH4III ACNGT 8 cut(s) 246, 586, 997, 1352, 1676, 1788, 1850, 2241
HpyCH4IV ACGT 2 cut(s) 546, 2360
HpyF10VI GCNNNNNNNGC 4 cut(s) 1113, 1561, 1933, 2212
HpyF3I CTNAG 5 cut(s) 165, 318, 1964, 2042, 2170
HpySE526I ACGT 2 cut(s) 546, 2360
Hsp92I GRCGYC 1 cut(s) 546
Hsp92II CATG 9 cut(s) 387, 668, 935, 1094, 1376, 1564, 1771, 1822, 1936
KpnI GGTACC 1 cut(s) 653
Ksp22I TGATCA 2 cut(s) 57, 1087
Kzo9I GATC 8 cut(s) 29, 57, 718, 807, 1007, 1032, 1087, 2230
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 3 cut(s) 496, 2137, 2358
Lsp1109I GCAGC 4 cut(s) 1033, 1128, 2055, 2153
LweI GCATC 3 cut(s) 871, 1114, 2228
MaeI CTAG 3 cut(s) 38, 215, 1166
MaeII ACGT 2 cut(s) 546, 2360
MaeIII GTNAC 6 cut(s) 295, 580, 814, 1363, 1663, 1940
MalI GATC 8 cut(s) 31, 59, 720, 809, 1009, 1034, 1089, 2232
MboI GATC 8 cut(s) 29, 57, 718, 807, 1007, 1032, 1087, 2230
MflI RGATCY 5 cut(s) 29, 718, 807, 1032, 2230
MhlI GDGCHC 1 cut(s) 164
MlyI GAGTC 2 cut(s) 1241, 2069
MmeI TCCRAC 5 cut(s) 69, 228, 397, 685, 982
Mph1103I ATGCAT 1 cut(s) 886
MroXI GAANNNNTTC 3 cut(s) 455, 765, 2288
MslI CAYNNNNRTG 2 cut(s) 1549, 2154
MspR9I CCNGG 3 cut(s) 495, 647, 1330
MvaI CCWGG 3 cut(s) 495, 647, 1330
MwoI GCNNNNNNNGC 4 cut(s) 1113, 1561, 1933, 2212
NdeII GATC 8 cut(s) 29, 57, 718, 807, 1007, 1032, 1087, 2230
NlaIII CATG 9 cut(s) 387, 668, 935, 1094, 1376, 1564, 1771, 1822, 1936
NlaIV GGNNCC 6 cut(s) 643, 651, 1326, 2087, 2285, 2354
NmuCI GTSAC 3 cut(s) 814, 1663, 1940
NsiI ATGCAT 1 cut(s) 886
NspI RCATGY 1 cut(s) 1564
PagI TCATGA 1 cut(s) 1090
PasI CCCWGGG 1 cut(s) 1329
PciSI GCTCTTC 1 cut(s) 615
PdmI GAANNNNTTC 3 cut(s) 455, 765, 2288
PfeI GAWTC 5 cut(s) 41, 755, 1082, 1415, 2080
PflMI CCANNNNNTGG 2 cut(s) 842, 1712
PfoI TCCNGGA 1 cut(s) 493
PkrI GCNGC 4 cut(s) 1023, 1118, 2070, 2168
PleI GAGTC 2 cut(s) 1240, 2069
PpsI GAGTC 2 cut(s) 1240, 2069
PpuMI RGGWCCY 1 cut(s) 1324
PshAI GACNNNNGTC 1 cut(s) 320
PshBI ATTAAT 1 cut(s) 1049
PsiI TTATAA 1 cut(s) 1310
Psp5II RGGWCCY 1 cut(s) 1324
Psp6I CCWGG 3 cut(s) 493, 645, 1328
PspEI GGTNACC 1 cut(s) 295
PspGI CCWGG 3 cut(s) 493, 645, 1328
PspN4I GGNNCC 6 cut(s) 643, 651, 1326, 2087, 2285, 2354
PspPI GGNCC 4 cut(s) 173, 322, 428, 1324
PspPPI RGGWCCY 1 cut(s) 1324
PsrI GAACNNNNNNTAC 4 cut(s) 364, 396, 1886, 1918
PsuI RGATCY 5 cut(s) 29, 718, 807, 1032, 2230
RsaI GTAC 6 cut(s) 126, 184, 651, 692, 1785, 2053
RsaNI GTAC 6 cut(s) 125, 183, 650, 691, 1784, 2052
RseI CAYNNNNRTG 2 cut(s) 1549, 2154
SapI GCTCTTC 1 cut(s) 615
SatI GCNGC 4 cut(s) 1022, 1117, 2069, 2167
Sau3AI GATC 8 cut(s) 29, 57, 718, 807, 1007, 1032, 1087, 2230
Sau96I GGNCC 4 cut(s) 173, 322, 428, 1324
ScaI AGTACT 1 cut(s) 184
SchI GAGTC 2 cut(s) 1241, 2069
ScrFI CCNGG 3 cut(s) 495, 647, 1330
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 3 cut(s) 871, 1114, 2228
SfcI CTRYAG 2 cut(s) 45, 1026
SinI GGWCC 3 cut(s) 173, 322, 1324
SmiMI CAYNNNNRTG 2 cut(s) 1549, 2154
SmlI CTYRAG 2 cut(s) 770, 2197
SmoI CTYRAG 2 cut(s) 770, 2197
SsiI CCGC 2 cut(s) 1229, 1929
SspI AATATT 3 cut(s) 310, 343, 2277
SspMI CTAG 3 cut(s) 38, 215, 1166
StyD4I CCNGG 3 cut(s) 493, 645, 1328
StyI CCWWGG 4 cut(s) 16, 291, 1128, 1171
TaaI ACNGT 8 cut(s) 246, 586, 997, 1352, 1676, 1788, 1850, 2241
TaiI ACGT 2 cut(s) 549, 2363
TaqI TCGA 3 cut(s) 1526, 1857, 2078
TatI WGTACW 4 cut(s) 124, 182, 1783, 2051
TfiI GAWTC 5 cut(s) 41, 755, 1082, 1415, 2080
TscAI CASTG 4 cut(s) 60, 466, 1002, 1357
TseFI GTSAC 3 cut(s) 814, 1663, 1940
TseI GCWGC 4 cut(s) 1021, 1116, 2068, 2166
Tsp45I GTSAC 3 cut(s) 814, 1663, 1940
TspGWI ACGGA 1 cut(s) 1496
TspRI CASTG 4 cut(s) 60, 466, 1002, 1357
Van91I CCANNNNNTGG 2 cut(s) 842, 1712
VpaK11BI GGWCC 3 cut(s) 173, 322, 1324
VspI ATTAAT 1 cut(s) 1049
XapI RAATTY 6 cut(s) 115, 557, 695, 761, 1625, 2101
XceI RCATGY 1 cut(s) 1564
XcmI CCANNNNNNNNNTGG 1 cut(s) 1294
XmnI GAANNNNTTC 3 cut(s) 455, 765, 2288
XspI CTAG 3 cut(s) 38, 215, 1166
ZraI GACGTC 1 cut(s) 547
ZrmI AGTACT 1 cut(s) 184
Zsp2I ATGCAT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.