Rw2G013280

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000039
Physical Location & Seq
Forward (+)
14017271 .. 14020893
3623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013280.1

Sequence Viewer

Length: 2646 bp
ATGGTTGGTGGAGGTGATGTTTTTGGTGGGGCTGCTGGAGGATTGCTGTTCAATGCTCTCTATGATGTGCTTAAAGAGCTCATCAGCAAGTCTATGATATATAGACCTCTTCTCAAAGACATTCAATCTTCGCTAGACTCATATCGACCACTGATCAAACAGATAGAAGCAAGCAATGAGGAACTGGGTTTTCCACAAGAGGAAGTAGAAAGATTCAAATTATATATGGAGGAGGGCTTAGAGATAGTCCAGAAGTGCTCTAAGGTTCGCAAGTGGAACATCTACAAGAAGTACAAGTACGCCAACAAACTTCTTGGATGGGATGCTGCTCTTGTAAGGCAGTTGGATGTGCTGAAGGTGCAGGGAGTAAGAGATGGGAAGAAGGCCTTGGCTTCGGTAGAGCAAATAAAGAGGGTGGTCACTGGGATTGAAGCGAGTAGTATCTTTTTAGGGGCAGGGGTTGCGCATATTGCAAAATCCAGTAGGCATATGGAGAAGGCGCTCAGCGGAGTTGAAATGAGAAGTGGAGATATAGTGGAGGGGATGTGCCGAATTGAAGACCGAGTTGAAACAAGCTGTAGCATTGTGGAGGCAGGGGTCAAGCGGATTGAAGACCGACTTGATACACGAAATGGAGCTAAAGTTGGAGCTTGGTGTGTTGTGCCTGAACTTACACAGTTTACGGTTGGATTGGATGAGCCTTTGAAGGAATTGAAGATGAAACTTTTTAAGGATGGAGTCTCGATGCTTGTGGTCACTGCTCCCGGAGGATGTGGGAAAACTACATTGGCAACAAAGTTTTGTCAAGATGAGCAAGTCAAAGGTAAATTCAAGAATAATATCTTCTTTGTCACTGTGTCAAACACACCAAACCTGAGCCTTATTGTGCAAGAGCTATACCAATGCAAGGGTTCCCAGGTGCCTACTTTCCAAGACGAAATAACTGCAGTCAAGTGGCTGCAAACATTTCTGAAGGAAGAAGGACAAAACCCTTTACTATTAGTCTTGGATGATGTTTGGTCTGGATCAGAATCCCTACTAGACAAGTTTCAGTTCAAGATGCCAGATTACAAGATTTTGGTCACATCAAGATCTGAATTTCCGAGATTTGGTTCCTCATATCATTTGCAATCGTTGGATTATGACAATGCCATGAAACTTTTTCATCATTCAGCATCTTTGGGAGATAAAAGCTCACATATTCCAGAATACCTCTCGAGTCAGATAGTAAAGGGCTGTAAGGGATTTCCACTTGCAATTACGGTGGCAGGAGGATCACTTTGCGGACAAAGAATAGAGATCTGGCAACAAATACTAATTGAATGGTCCAGAGGGTCTTCTATTCTTGATTCTGAGACCAAATTGCTTCTTCGCCTCCAAAGCAGCTTAGTTGTCTCGGACTCGGATAAAGAAATGGCTATCATCAGGAAGTGTTTCTTAGACCTTGCTTCATTTCCTGAAGACCAAAGAATCCCAGTTACTGCCCTCATTGATATGTGGACAGAGTCATATGATCAGCTAGATGAAGATACTTTGTGTGTTGCATACCTCCAGAGGCTGACTAACCGAAGTCTGGCTAATCTTGTAGTCACACGGAAGGACCAGAGCCTGGGGGATGGATACTACAGCGAACATTTTGTTACCCAGCACGATCTGCTTAGAGAGTTGGCTATCTATGAGACAAAACAAGAAGACCCAGGACATAGAGAAAGACTGATTATAAACATATCTGGAGACAATTTTCCCAAGTGGTGGAGTGAAAAGAAGCATGAATCCTTGAAAGCTCGCTTATTATCCATCTCAACTGATGGAAATTTCTCATCAAAATGGCACCATATGGAACTACCATATGCTGAGGTTCTAGTCTTGAATTTTCAGACGAAGAACTACGCCTTACCCAAATTTGTGGACACTATGCGTAAATTGAAGGTACTTGTTGTCACAAATTATGGTTTCTCGCCTGCTGAACTAAGTAATATCCCACTACTGGGTTCATTATCTCATCTAAAGAGAATTAGATTGGAGCGTGTCTCAATACCATCAATGTCCAAGAACCCCATACAATTGGTGGGTCTAAAAAAGATATCTCTATTCATGTGTACCATTGGGAAAGCTTTCGGTGACGGTTCCATCCAGATTTCATACGCATTTCCAAATCTAGAGGAAATTAGCATTGACTATTGCAATGACTTGGTGGAAATGCCTGCCGACGTCTGTGATCTTAGTCATCTACGGAAGCTCAGTATCACCAACTCACATAAGCTTCTTGCCTTGCCTGAAGAAATTGGAAAACTGGAAAAGTTGGAACTGCTGAGACTAAGGTCATGTACAGACTTGGCACAGTTGCCAGGCTCGAGCAGGAACCTAAAGAAGTTGATCTTTCTTGACATTTCTTATTGCTTTAGCATCAAGGAGTTGCCTGGAGATATTGGTGAAATGAGCAGCTTAAGAAAGCTCAACATGAGAGAATGCTCAAGATTGCAAGAAGTGCCTGTGTCGGTTTTGGATCTTGAGCATTTGGAGGAAGTGATATGTGATGACGAGACAAGAAATTTATGGGAACCATTCTTACCTCTCGGAAACACAAGAATAGTGGTGGAAAAAGAAAATATCAACCTAAATTGGCTCGATAAGCTTCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

881

Amino Acids

99.5

Weight (kDa)

6.23

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 136 3.9e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 233 - 390 1e-15 NB-ARC domain
LRR_14 PF23598 735 - 841 2.1e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1721
AatII GACGTC 1 cut(s) 2214
Acc16I TGCGCA 1 cut(s) 465
AccB1I GGYRCC 2 cut(s) 919, 1830
AccB7I CCANNNNNTGG 2 cut(s) 1609, 1752
AciI CCGC 3 cut(s) 507, 604, 1284
AclWI GGATC 3 cut(s) 1033, 1282, 2514
AcsI RAATTY 6 cut(s) 827, 1097, 1813, 1870, 1901, 2551
AcuI CTGAAG 4 cut(s) 374, 992, 1479, 2298
AcyI GRCGYC 1 cut(s) 2211
AfaI GTAC 5 cut(s) 293, 299, 1932, 2101, 2329
AfiI CCNNNNNNNGG 7 cut(s) 907, 1109, 1573, 1609, 1752, 1987, 1988
AflII CTTAAG 1 cut(s) 2446
AjnI CCWGG 5 cut(s) 915, 1608, 1696, 2347, 2419
AjuI GAANNNNNNNTTGG 4 cut(s) 371, 403, 770, 802
Alw21I GWGCWC 2 cut(s) 81, 260
Alw26I GTCTC 8 cut(s) 745, 1349, 1399, 1673, 1728, 2035, 2308, 2537
AlwI GGATC 3 cut(s) 1033, 1282, 2514
AlwNI CAGNNNCTG 3 cut(s) 1481, 1558, 1609
Ama87I CYCGRG 2 cut(s) 1216, 2353
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 5 cut(s) 32, 326, 958, 1383, 2442
ApoI RAATTY 6 cut(s) 827, 1097, 1813, 1870, 1901, 2551
Asp700I GAANNNNTTC 2 cut(s) 1433, 2114
AspLEI GCGC 2 cut(s) 466, 502
AspS9I GGNCC 2 cut(s) 1326, 1600
AsuC2I CCSGG 1 cut(s) 765
AsuHPI GGTGA 4 cut(s) 26, 2132, 2239, 2444
AvaI CYCGRG 2 cut(s) 1216, 2353
AvaII GGWCC 2 cut(s) 1326, 1600
BanI GGYRCC 2 cut(s) 919, 1830
BanII GRGCYC 1 cut(s) 81
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
BbsI GAAGAC 5 cut(s) 564, 618, 1329, 1467, 1698
Bbv12I GWGCWC 2 cut(s) 81, 260
BbvCI CCTCAGC 1 cut(s) 1854
BbvI GCAGC 5 cut(s) 19, 313, 945, 1395, 2454
BccI CCATC 8 cut(s) 312, 368, 728, 1610, 1802, 1805, 2047, 2138
BcgI CGANNNNNNTGC 2 cut(s) 926, 960
BciT130I CCWGG 5 cut(s) 917, 1610, 1698, 2349, 2421
BciVI GTATCC 1 cut(s) 1613
BclI TGATCA 2 cut(s) 153, 1513
BcnI CCSGG 1 cut(s) 765
BcoDI GTCTC 8 cut(s) 745, 1349, 1399, 1673, 1728, 2035, 2308, 2537
BfaI CTAG 5 cut(s) 134, 1040, 1520, 1862, 2159
BfmI CTRYAG 3 cut(s) 577, 945, 1624
BfoI RGCGCY 1 cut(s) 503
BfrI CTTAAG 1 cut(s) 2446
BfuI GTATCC 1 cut(s) 1613
BglII AGATCT 2 cut(s) 1091, 1299
BisI GCNGC 5 cut(s) 33, 327, 959, 1384, 2443
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 5 cut(s) 34, 328, 960, 1385, 2444
Bme1390I CCNGG 6 cut(s) 765, 917, 1610, 1698, 2349, 2421
Bme18I GGWCC 2 cut(s) 1326, 1600
BmeT110I CYCGRG 2 cut(s) 1216, 2353
BmgT120I GGNCC 2 cut(s) 1326, 1600
BmiI GGNNCC 7 cut(s) 913, 921, 1114, 1832, 2128, 2363, 2562
BmrFI CCNGG 6 cut(s) 765, 917, 1610, 1698, 2349, 2421
BmrI ACTGGG 4 cut(s) 194, 432, 1469, 1997
BmsI GCATC 5 cut(s) 313, 735, 1050, 1184, 2415
BmuI ACTGGG 4 cut(s) 194, 432, 1469, 1997
BoxI GACNNNNGTC 1 cut(s) 2320
BpiI GAAGAC 5 cut(s) 564, 618, 1329, 1467, 1698
BplI GAGNNNNNCTC 2 cut(s) 2015, 2047
BpmI CTGGAG 4 cut(s) 57, 1535, 1752, 2442
Bpu10I CCTNAGC 2 cut(s) 875, 1854
Bpu1102I GCTNAGC 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 2458, 2531
BpuMI CCSGG 1 cut(s) 765
BsaBI GATNNNNATC 1 cut(s) 1030
BsaHI GRCGYC 1 cut(s) 2211
BsaI GGTCTC 1 cut(s) 1349
BsaJI CCNNGG 4 cut(s) 387, 915, 1609, 1696
BsaXI ACNNNNNCTCC 7 cut(s) 33, 519, 530, 549, 560, 627, 657
Bsc4I CCNNNNNNNGG 7 cut(s) 907, 1109, 1573, 1609, 1752, 1987, 1988
Bse1I ACTGG 6 cut(s) 189, 427, 480, 1475, 1992, 2298
Bse3DI GCAATG 2 cut(s) 181, 2191
Bse8I GATNNNNATC 1 cut(s) 1030
BseBI CCWGG 5 cut(s) 917, 1610, 1698, 2349, 2421
BseDI CCNNGG 4 cut(s) 387, 915, 1609, 1696
BseJI GATNNNNATC 1 cut(s) 1030
BseLI CCNNNNNNNGG 7 cut(s) 907, 1109, 1573, 1609, 1752, 1987, 1988
BseMI GCAATG 2 cut(s) 181, 2191
BseMII CTCAG 6 cut(s) 517, 866, 1344, 1845, 2254, 2303
BseNI ACTGG 6 cut(s) 189, 427, 480, 1475, 1992, 2298
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 5 cut(s) 19, 313, 945, 1395, 2454
BseYI CCCAGC 1 cut(s) 1644
BsgI GTGCAG 1 cut(s) 380
BshFI GGCC 1 cut(s) 386
BshNI GGYRCC 2 cut(s) 919, 1830
BsiHKAI GWGCWC 2 cut(s) 81, 260
BsiHKCI CYCGRG 2 cut(s) 1216, 2353
BsiSI CCGG 1 cut(s) 765
BslI CCNNNNNNNGG 7 cut(s) 907, 1109, 1573, 1609, 1752, 1987, 1988
BsmAI GTCTC 8 cut(s) 745, 1349, 1399, 1673, 1728, 2035, 2308, 2537
BsmI GAATGC 1 cut(s) 2474
BsnI GGCC 1 cut(s) 386
Bso31I GGTCTC 1 cut(s) 1349
BsoBI CYCGRG 2 cut(s) 1216, 2353
Bsp1286I GDGCHC 2 cut(s) 81, 260
Bsp1407I TGTACA 1 cut(s) 2327
Bsp1720I GCTNAGC 1 cut(s) 503
BspACI CCGC 3 cut(s) 507, 604, 1284
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 6 cut(s) 516, 867, 1345, 1846, 2253, 2304
BspLI GGNNCC 7 cut(s) 913, 921, 1114, 1832, 2128, 2363, 2562
BspMAI CTGCAG 1 cut(s) 949
BspPI GGATC 3 cut(s) 1033, 1282, 2514
BspT107I GGYRCC 2 cut(s) 919, 1830
BspTI CTTAAG 1 cut(s) 2446
BspTNI GGTCTC 1 cut(s) 1349
BsrDI GCAATG 2 cut(s) 181, 2191
BsrGI TGTACA 1 cut(s) 2327
BsrI ACTGG 6 cut(s) 189, 427, 480, 1475, 1992, 2298
BssECI CCNNGG 4 cut(s) 387, 915, 1609, 1696
BssNI GRCGYC 1 cut(s) 2211
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 5 cut(s) 917, 1610, 1698, 2349, 2421
Bst4CI ACNGT 6 cut(s) 678, 685, 856, 1264, 2126, 2343
Bst6I CTCTTC 1 cut(s) 114
BstACI GRCGYC 1 cut(s) 2211
BstAFI CTTAAG 1 cut(s) 2446
BstAPI GCANNNNNTGC 3 cut(s) 461, 1654, 2488
BstAUI TGTACA 1 cut(s) 2327
BstC8I GCNNGC 4 cut(s) 172, 1786, 1962, 2205
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 2 cut(s) 466, 502
BstMAI GTCTC 8 cut(s) 745, 1349, 1399, 1673, 1728, 2035, 2308, 2537
BstMWI GCNNNNNNNGC 8 cut(s) 76, 358, 461, 470, 1380, 1654, 2488, 2632
BstNI CCWGG 5 cut(s) 917, 1610, 1698, 2349, 2421
BstPAI GACNNNNGTC 1 cut(s) 2320
BstSCI CCNGG 6 cut(s) 763, 915, 1608, 1696, 2347, 2419
BstSFI CTRYAG 3 cut(s) 577, 945, 1624
BstV1I GCAGC 5 cut(s) 19, 313, 945, 1395, 2454
BstV2I GAAGAC 5 cut(s) 564, 618, 1329, 1467, 1698
BstX2I RGATCY 3 cut(s) 1091, 1299, 2506
BstXI CCANNNNNNTGG 2 cut(s) 1906, 2065
BstYI RGATCY 3 cut(s) 1091, 1299, 2506
BsuI GTATCC 1 cut(s) 1613
BsuRI GGCC 1 cut(s) 386
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 4 cut(s) 149, 420, 756, 852
Cac8I GCNNGC 4 cut(s) 172, 1786, 1962, 2205
CaiI CAGNNNCTG 3 cut(s) 1481, 1558, 1609
CfoI GCGC 2 cut(s) 466, 502
Cfr13I GGNCC 2 cut(s) 1326, 1600
Csp6I GTAC 5 cut(s) 292, 298, 1931, 2100, 2328
CspCI CAANNNNNGTGG 4 cut(s) 1245, 1280, 2574, 2609
CviAII CATG 5 cut(s) 1153, 1769, 2095, 2325, 2461
CviQI GTAC 5 cut(s) 292, 298, 1931, 2100, 2328
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 387
Eco147I AGGCCT 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 1349
Eco32I GATATC 1 cut(s) 2085
Eco47I GGWCC 2 cut(s) 1326, 1600
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 4 cut(s) 374, 992, 1479, 2298
Eco88I CYCGRG 2 cut(s) 1216, 2353
EcoICRI GAGCTC 1 cut(s) 79
EcoRII CCWGG 5 cut(s) 915, 1608, 1696, 2347, 2419
EcoRV GATATC 1 cut(s) 2085
EcoT14I CCWWGG 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 1156, 1772, 2098, 2328, 2464
FalI AAGNNNNNCTT 8 cut(s) 371, 403, 603, 635, 1421, 1453, 2363, 2395
FatI CATG 5 cut(s) 1152, 1768, 2094, 2324, 2460
FauNDI CATATG 4 cut(s) 489, 1510, 1836, 1849
FbaI TGATCA 2 cut(s) 153, 1513
Fnu4HI GCNGC 5 cut(s) 33, 327, 959, 1384, 2443
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 5 cut(s) 33, 327, 959, 1384, 2443
FspBI CTAG 5 cut(s) 134, 1040, 1520, 1862, 2159
FspI TGCGCA 1 cut(s) 465
GlaI GCGC 2 cut(s) 465, 501
GluI GCNGC 5 cut(s) 33, 327, 959, 1384, 2443
GsaI CCCAGC 1 cut(s) 1648
GsuI CTGGAG 4 cut(s) 57, 1535, 1752, 2442
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 1 cut(s) 386
HapII CCGG 1 cut(s) 765
HhaI GCGC 2 cut(s) 466, 502
Hin1I GRCGYC 1 cut(s) 2211
Hin1II CATG 5 cut(s) 1156, 1772, 2098, 2328, 2464
Hin6I GCGC 2 cut(s) 464, 500
HinP1I GCGC 2 cut(s) 464, 500
HindIII AAGCTT 3 cut(s) 2112, 2261, 2633
HpaII CCGG 1 cut(s) 765
HphI GGTGA 4 cut(s) 26, 2132, 2239, 2444
Hpy166II GTNNAC 4 cut(s) 681, 1500, 1909, 2100
Hpy8I GTNNAC 4 cut(s) 681, 1500, 1909, 2100
Hpy99I CGWCG 1 cut(s) 2213
HpyAV CCTTC 8 cut(s) 349, 376, 490, 700, 967, 974, 1591, 1921
HpyCH4III ACNGT 6 cut(s) 678, 685, 856, 1264, 2126, 2343
HpyCH4IV ACGT 1 cut(s) 2211
HpyF10VI GCNNNNNNNGC 8 cut(s) 76, 358, 461, 470, 1380, 1654, 2488, 2632
HpySE526I ACGT 1 cut(s) 2211
Hsp92I GRCGYC 1 cut(s) 2211
Hsp92II CATG 5 cut(s) 1156, 1772, 2098, 2328, 2464
HspAI GCGC 2 cut(s) 464, 500
Ksp22I TGATCA 2 cut(s) 153, 1513
LmnI GCTCC 4 cut(s) 635, 647, 766, 2023
Lsp1109I GCAGC 5 cut(s) 19, 313, 945, 1395, 2454
LweI GCATC 5 cut(s) 313, 735, 1050, 1184, 2415
MaeI CTAG 5 cut(s) 134, 1040, 1520, 1862, 2159
MaeII ACGT 1 cut(s) 2211
MaeIII GTNAC 9 cut(s) 418, 754, 850, 1081, 1477, 1588, 1639, 1939, 2120
MfeI CAATTG 1 cut(s) 2063
MflI RGATCY 3 cut(s) 1091, 1299, 2506
MhlI GDGCHC 2 cut(s) 81, 260
MlyI GAGTC 5 cut(s) 131, 747, 1228, 1394, 1514
MmeI TCCRAC 5 cut(s) 324, 625, 667, 1116, 2283
MroXI GAANNNNTTC 2 cut(s) 1433, 2114
MseI TTAA 3 cut(s) 72, 729, 2447
MslI CAYNNNNRTG 2 cut(s) 1493, 1825
MspA1I CMGCKG 1 cut(s) 507
MspCI CTTAAG 1 cut(s) 2446
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 6 cut(s) 765, 917, 1610, 1698, 2349, 2421
MunI CAATTG 1 cut(s) 2063
Mva1269I GAATGC 1 cut(s) 2474
MvaI CCWGG 5 cut(s) 917, 1610, 1698, 2349, 2421
MwoI GCNNNNNNNGC 8 cut(s) 76, 358, 461, 470, 1380, 1654, 2488, 2632
NciI CCSGG 1 cut(s) 765
NdeI CATATG 4 cut(s) 489, 1510, 1836, 1849
NlaIII CATG 5 cut(s) 1156, 1772, 2098, 2328, 2464
NlaIV GGNNCC 7 cut(s) 913, 921, 1114, 1832, 2128, 2363, 2562
NmuCI GTSAC 7 cut(s) 418, 754, 850, 1081, 1588, 1939, 2120
NsbI TGCGCA 1 cut(s) 465
PaeR7I CTCGAG 2 cut(s) 1216, 2353
PceI AGGCCT 1 cut(s) 386
PctI GAATGC 1 cut(s) 2474
PdmI GAANNNNTTC 2 cut(s) 1433, 2114
PfeI GAWTC 5 cut(s) 213, 1031, 1349, 1470, 1772
PflFI GACNNNGTC 1 cut(s) 1504
PflMI CCANNNNNTGG 2 cut(s) 1609, 1752
PfoI TCCNGGA 1 cut(s) 763
PkrI GCNGC 5 cut(s) 34, 328, 960, 1385, 2444
PleI GAGTC 5 cut(s) 131, 746, 1227, 1394, 1513
PpsI GAGTC 5 cut(s) 131, 746, 1227, 1394, 1513
PshAI GACNNNNGTC 1 cut(s) 2320
PsiI TTATAA 1 cut(s) 1721
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 5 cut(s) 915, 1608, 1696, 2347, 2419
PspFI CCCAGC 1 cut(s) 1644
PspGI CCWGG 5 cut(s) 915, 1608, 1696, 2347, 2419
PspN4I GGNNCC 7 cut(s) 913, 921, 1114, 1832, 2128, 2363, 2562
PspPI GGNCC 2 cut(s) 1326, 1600
PspXI VCTCGAGB 1 cut(s) 2353
PsrI GAACNNNNNNTAC 2 cut(s) 2553, 2585
PstI CTGCAG 1 cut(s) 949
PstNI CAGNNNCTG 3 cut(s) 1481, 1558, 1609
PsuI RGATCY 3 cut(s) 1091, 1299, 2506
PsyI GACNNNGTC 1 cut(s) 1504
RsaI GTAC 5 cut(s) 293, 299, 1932, 2101, 2329
RsaNI GTAC 5 cut(s) 292, 298, 1931, 2100, 2328
RseI CAYNNNNRTG 2 cut(s) 1493, 1825
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 72, 729, 2447
SatI GCNGC 5 cut(s) 33, 327, 959, 1384, 2443
Sau96I GGNCC 2 cut(s) 1326, 1600
SchI GAGTC 5 cut(s) 131, 747, 1228, 1394, 1514
ScrFI CCNGG 6 cut(s) 765, 917, 1610, 1698, 2349, 2421
SduI GDGCHC 2 cut(s) 81, 260
SfaNI GCATC 5 cut(s) 313, 735, 1050, 1184, 2415
SfcI CTRYAG 3 cut(s) 577, 945, 1624
Sfr274I CTCGAG 2 cut(s) 1216, 2353
SinI GGWCC 2 cut(s) 1326, 1600
SlaI CTCGAG 2 cut(s) 1216, 2353
SmiMI CAYNNNNRTG 2 cut(s) 1493, 1825
SmlI CTYRAG 5 cut(s) 1216, 2353, 2446, 2473, 2510
SmoI CTYRAG 5 cut(s) 1216, 2353, 2446, 2473, 2510
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 3 cut(s) 507, 604, 1284
SspMI CTAG 5 cut(s) 134, 1040, 1520, 1862, 2159
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 6 cut(s) 763, 915, 1608, 1696, 2347, 2419
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 6 cut(s) 678, 685, 856, 1264, 2126, 2343
TaiI ACGT 1 cut(s) 2214
TaqI TCGA 5 cut(s) 145, 743, 1217, 2354, 2628
TaqII GACCGA 2 cut(s) 576, 630
TatI WGTACW 2 cut(s) 291, 2327
TfiI GAWTC 5 cut(s) 213, 1031, 1349, 1470, 1772
Tru1I TTAA 3 cut(s) 72, 729, 2447
Tru9I TTAA 3 cut(s) 72, 729, 2447
TscAI CASTG 4 cut(s) 156, 427, 763, 859
TseFI GTSAC 7 cut(s) 418, 754, 850, 1081, 1588, 1939, 2120
TseI GCWGC 5 cut(s) 32, 326, 958, 1383, 2442
Tsp45I GTSAC 7 cut(s) 418, 754, 850, 1081, 1588, 1939, 2120
TspDTI ATGAA 9 cut(s) 734, 1154, 1169, 1440, 1539, 1785, 1983, 2083, 2130
TspGWI ACGGA 2 cut(s) 1609, 2248
TspRI CASTG 4 cut(s) 156, 427, 763, 859
Tth111I GACNNNGTC 1 cut(s) 1504
Van91I CCANNNNNTGG 2 cut(s) 1609, 1752
Vha464I CTTAAG 1 cut(s) 2446
VpaK11BI GGWCC 2 cut(s) 1326, 1600
XapI RAATTY 6 cut(s) 827, 1097, 1813, 1870, 1901, 2551
XbaI TCTAGA 1 cut(s) 2158
XcmI CCANNNNNNNNNTGG 2 cut(s) 487, 2065
XhoI CTCGAG 2 cut(s) 1216, 2353
XmnI GAANNNNTTC 2 cut(s) 1433, 2114
XspI CTAG 5 cut(s) 134, 1040, 1520, 1862, 2159
ZraI GACGTC 1 cut(s) 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.