Rh2BG177900

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000039
Physical Location & Seq
Forward (+)
15798294 .. 15803790
5497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG177900.1

Sequence Viewer

Length: 2541 bp
ATGGCTTCTGAGGGCGCTACGAAATCGAGCTCAGCGGCCCTCGGCTCCGTCGTCGTGGTTGGCTCCGCTGGAGCCGGTGATGGCCGCGAAGCCGCAAGAGAAAACAAGGCAGTGGCATTGATCGGAGGAACTGGTTTGCCAGTAACATTTTGGACGCTGTGTGATTCTGTTAAGCAAATGAAGGATATACTGATGTTCAAACCCCAACTAAGGGATATCAAATCCACTTTGGACTCTTTGGAACCACTGATCAAAGAGATGGCAGAGTGTGAGAAGGAATTGGATGGTCAAGAGGAGGAGCTAGAGAATCTTAGAGTGGAAATGGAGGGAGGAGTGGAACTTATCCGCAAGTGCTCGAAAGCAAGCAGGTTCAAGCAGTATAAGTACGGCAGCAGGCTTGTTGAATTCAGCAAGTCACTTCAGAGGCTGTTGGGGATATTGAAAGTGCAGGGAATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGAAATATCGAGAATGTGGTGCATAGAATTGAAGGAAATTGTGTGACACAGAACAATCAGTCTGAAAAAATTGCGGGGTGGTGTGCTGTGCCTGAGCCTCCAACGATTACTGTTGGATTGGATGTGCCTTTGTGGGAATTGAAGAAGAAGCTTTTTACTGACGGAGTATCGATGGTGATAATTACTGCTCCTGGAGGATGTGGAAAAACTACATTGGCAACAAAGTTTTGCAAGGATGCAGAAGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACTTATACCTAATTGTACAAGAGCTATATCATGGAACGGGTGCCCAGGTCCCCGATTTCCAAAATGAAGCAACTGCAGTTAGCTGGCTGCAGCAATTTCTGAAGCAAACAGGACAAAATCCTATGTTGTTGGTCCTGGATGATGTCTGGGCAGGATCAGAATTCATTCTTGAGAAGTTTGATGAATTAAAGATGTCAGATTACAAGATATTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTATTTAGAATGTTTGAACAACAAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGTGATAGGAGCTCTTATATTTCAGAAGATCTTCAGAGAAAGATAGTAGAACGTTGTATGGGATTTCCACTTGCAATTACAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTACTGGAATGGTCTAGAGGTTGTTCTGTACTTGAGTCAGAACATGATTTGCTTGATTGCCTCCAAAGAAGCTTAGATGCTTTGGATGAAGAGAAGTCTCTTATCAGGGAATGCTTCATAGACCTTGGTTTATTTCCTGAAGATAAAAGAATCTCAGCTACTGTCCTCATTGATATGTGGGCAGAGTTATATGGCATAAATGAGGACATTTCGTCCATTGTGAACCTCTATGAACTTGCCTCTCGGAGTTTGGTTAATCTTGTACTCACAAGGAATGACAAGGATGGCGATGGCTATTACACTGAACACTTTGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCAACAGATTAGTCGGGAACCACTACAACAGAGAATGAGACTTATGATTGACATAAGTGGAGACAACTTTCCTATGTGGTGGAAAGAACAAAAGTATCAATCCCTCATAGCTCGCCTTGTATCTATCTCAACAGACGAAGAGTTCTCATCTAAATGGCAAAACTTGCCACTACCAGAAGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTGGAGGAAATGAATAAACTGAAGACTCTAGTACTCACAAGTTCAGGTATCGGTCCTGCTGAGTTGAATAACTTTCAACTAGTTGATTCGAAACCAAATCTAAAGAGAATCAGACTAGAACGAATTTCATTTCCTTCATTAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTAGCGTTGGCCAAGGTTTTGGTAACGGTTCCATACATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGTTGCCTGCCGATCTCTGTGACCTTATTCAACTGAAGAAGCTCAGTGTCACCAATTCTCATAAGCTCTCTGTCTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGCTGCTGAGGCTTAGGTGTTGTACAGACTTGTCAGAGTTACCCGGCTCGATTAGGAAGCTCAAGAACTTAAACTTGCTTGACATATCGAATTGCTTTAGCCTTAAGGAATTGCCTGAAGACATTGGAGAAATGTGCAGTTTAGAGAAGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCATCAGTTGTGGATCTTGAAAAGTTAATGGAAGTGACATGTGATGAAGTGACAGAAATCTTATGGGAGCCTTGCTTGCCGTATCTCAGTAACATACACATAAAGGTTTTGAAAGAAGATATCAACCTGAATTGGCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

846

Amino Acids

95.54

Weight (kDa)

5.31

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 40 - 164 5.4e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 208 - 362 1.4e-15 NB-ARC domain
AAA_22 PF13401 219 - 314 7e-07 AAA domain
LRR_14 PF23598 685 - 768 6.8e-09 Leucine-rich repeat region
LRR_14 PF23598 770 - 818 6.8e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1006
Acc36I ACCTGC 2 cut(s) 357, 1944
AccB1I GGYRCC 1 cut(s) 824
AccB7I CCANNNNNTGG 1 cut(s) 1025
AccII CGCG 1 cut(s) 87
AciI CCGC 7 cut(s) 35, 66, 85, 93, 346, 560, 1200
AclI AACGTT 1 cut(s) 1150
AclWI GGATC 2 cut(s) 946, 2418
AcoI YGGCCR 2 cut(s) 82, 2011
AcsI RAATTY 5 cut(s) 404, 740, 944, 1932, 2203
AcuI CTGAAG 8 cut(s) 404, 905, 1115, 1389, 1850, 2156, 2202, 2346
AfaI GTAC 7 cut(s) 386, 801, 1233, 1260, 1494, 1842, 2233
AfiI CCNNNNNNNGG 3 cut(s) 210, 211, 1025
AflII CTTAAG 1 cut(s) 2312
AflIII ACRYGT 1 cut(s) 2435
AhdI GACNNNNNGTC 1 cut(s) 1441
AhlI ACTAGT 1 cut(s) 1888
AjnI CCWGG 3 cut(s) 676, 828, 918
AjuI GAANNNNNNNTTGG 4 cut(s) 458, 490, 1012, 1044
Alw21I GWGCWC 4 cut(s) 32, 356, 1112, 2378
Alw26I GTCTC 4 cut(s) 1332, 1609, 1632, 2362
AlwI GGATC 2 cut(s) 946, 2418
AlwNI CAGNNNCTG 2 cut(s) 427, 1391
Ama87I CYCGRG 1 cut(s) 2377
AoxI GGCC 3 cut(s) 36, 82, 2011
ApeKI GCWGC 6 cut(s) 390, 871, 874, 1088, 1204, 2212
ApoI RAATTY 5 cut(s) 404, 740, 944, 1932, 2203
Asp700I GAANNNNTTC 2 cut(s) 948, 1128
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 4 cut(s) 37, 832, 916, 1862
AsuC2I CCSGG 1 cut(s) 2253
AsuHPI GGTGA 7 cut(s) 89, 496, 673, 1009, 1073, 1112, 2143
AsuII TTCGAA 1 cut(s) 1898
AvaI CYCGRG 1 cut(s) 2377
AvaII GGWCC 3 cut(s) 832, 916, 1862
AxyI CCTNAGG 1 cut(s) 1096
BaeGI GKGCMC 1 cut(s) 829
BaeI ACNNNNGTAYC 2 cut(s) 1655, 1688
BalI TGGCCA 1 cut(s) 2013
BanI GGYRCC 1 cut(s) 824
BanII GRGCYC 2 cut(s) 32, 1112
BbsI GAAGAC 4 cut(s) 748, 1838, 2190, 2334
Bbv12I GWGCWC 4 cut(s) 32, 356, 1112, 2378
BbvCI CCTCAGC 1 cut(s) 2216
BbvI GCAGC 6 cut(s) 402, 858, 886, 1100, 1216, 2199
BccI CCATC 6 cut(s) 74, 253, 278, 652, 1508, 1514
BceAI ACGGC 2 cut(s) 403, 2461
BcgI CGANNNNNNTGC 2 cut(s) 462, 496
BciT130I CCWGG 3 cut(s) 678, 830, 920
BciVI GTATCC 1 cut(s) 1968
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 1 cut(s) 2253
BcoDI GTCTC 4 cut(s) 1332, 1609, 1632, 2362
BcuI ACTAGT 1 cut(s) 1888
BfaI CTAG 7 cut(s) 302, 1245, 1838, 1889, 1925, 2198, 2539
BfmI CTRYAG 2 cut(s) 858, 872
BfoI RGCGCY 1 cut(s) 18
BfrI CTTAAG 1 cut(s) 2312
BfuAI ACCTGC 2 cut(s) 357, 1944
BfuI GTATCC 1 cut(s) 1968
BglII AGATCT 1 cut(s) 1126
BisI GCNGC 9 cut(s) 36, 85, 93, 391, 872, 875, 1089, 1205, 2213
BlpI GCTNAGC 1 cut(s) 31
BlsI GCNGC 9 cut(s) 37, 86, 94, 392, 873, 876, 1090, 1206, 2214
BmcAI AGTACT 2 cut(s) 1233, 1842
Bme1390I CCNGG 4 cut(s) 678, 830, 920, 2253
Bme18I GGWCC 3 cut(s) 832, 916, 1862
BmeRI GACNNNNNGTC 1 cut(s) 1441
BmeT110I CYCGRG 1 cut(s) 2377
BmgT120I GGNCC 4 cut(s) 37, 832, 916, 1862
BmrFI CCNGG 4 cut(s) 678, 830, 920, 2253
BmsI GCATC 4 cut(s) 712, 1054, 1100, 1297
BpiI GAAGAC 4 cut(s) 748, 1838, 2190, 2334
BpmI CTGGAG 2 cut(s) 90, 699
Bpu10I CCTNAGC 4 cut(s) 579, 1562, 2216, 2222
Bpu1102I GCTNAGC 1 cut(s) 31
Bpu14I TTCGAA 1 cut(s) 1898
BpuEI CTTGAG 3 cut(s) 974, 1283, 2255
BpuMI CCSGG 1 cut(s) 2253
Bsa29I ATCGAT 1 cut(s) 656
BsaJI CCNNGG 4 cut(s) 40, 828, 1354, 2014
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 211, 1025
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 136, 140, 1239
Bse21I CCTNAGG 1 cut(s) 1096
Bse3DI GCAATG 3 cut(s) 757, 1074, 2095
BseBI CCWGG 3 cut(s) 678, 830, 920
BseCI ATCGAT 1 cut(s) 656
BseDI CCNNGG 4 cut(s) 40, 828, 1354, 2014
BseGI GGATG 9 cut(s) 289, 466, 613, 689, 727, 928, 1091, 1321, 1519
BseLI CCNNNNNNNGG 3 cut(s) 210, 211, 1025
BseMI GCAATG 3 cut(s) 757, 1074, 2095
BseMII CTCAG 8 cut(s) 45, 570, 1398, 1794, 1860, 2158, 2207, 2497
BseNI ACTGG 3 cut(s) 136, 140, 1239
BseRI GAGGAG 3 cut(s) 308, 311, 345
BseSI GKGCMC 1 cut(s) 829
BseXI GCAGC 6 cut(s) 402, 858, 886, 1100, 1216, 2199
BsgI GTGCAG 2 cut(s) 467, 2365
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 3 cut(s) 38, 84, 2013
BshNI GGYRCC 1 cut(s) 824
BshVI ATCGAT 1 cut(s) 656
BsiHKAI GWGCWC 4 cut(s) 32, 356, 1112, 2378
BsiHKCI CYCGRG 1 cut(s) 2377
BsiSI CCGG 2 cut(s) 75, 2253
BslFI GGGAC 1 cut(s) 818
BslI CCNNNNNNNGG 3 cut(s) 210, 211, 1025
BsmAI GTCTC 4 cut(s) 1332, 1609, 1632, 2362
BsmFI GGGAC 1 cut(s) 818
BsmI GAATGC 1 cut(s) 1346
BsnI GGCC 3 cut(s) 38, 84, 2013
BsoBI CYCGRG 1 cut(s) 2377
Bsp119I TTCGAA 1 cut(s) 1898
Bsp1286I GDGCHC 5 cut(s) 32, 356, 829, 1112, 2378
Bsp1407I TGTACA 2 cut(s) 799, 2231
Bsp143I GATC 7 cut(s) 120, 249, 938, 1126, 1190, 2113, 2410
Bsp1720I GCTNAGC 1 cut(s) 31
BspACI CCGC 7 cut(s) 35, 66, 85, 93, 346, 560, 1200
BspANI GGCC 3 cut(s) 38, 84, 2013
BspCNI CTCAG 8 cut(s) 44, 571, 1397, 1795, 1861, 2157, 2208, 2496
BspDI ATCGAT 1 cut(s) 656
BspFNI CGCG 1 cut(s) 87
BspMAI CTGCAG 2 cut(s) 862, 876
BspMI ACCTGC 2 cut(s) 357, 1944
BspPI GGATC 2 cut(s) 946, 2418
BspT104I TTCGAA 1 cut(s) 1898
BspT107I GGYRCC 1 cut(s) 824
BspTI CTTAAG 1 cut(s) 2312
BsrDI GCAATG 3 cut(s) 757, 1074, 2095
BsrFI RCCGGY 1 cut(s) 74
BsrGI TGTACA 2 cut(s) 799, 2231
BsrI ACTGG 3 cut(s) 136, 140, 1239
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 4 cut(s) 40, 828, 1354, 2014
BssMI GATC 7 cut(s) 120, 249, 938, 1126, 1190, 2113, 2410
BssT1I CCWWGG 2 cut(s) 1354, 2014
Bst2UI CCWGG 3 cut(s) 678, 830, 920
Bst4CI ACNGT 7 cut(s) 598, 769, 1180, 1393, 1968, 2030, 2373
Bst6I CTCTTC 2 cut(s) 1314, 1710
BstAFI CTTAAG 1 cut(s) 2312
BstAPI GCANNNNNTGC 2 cut(s) 1558, 1741
BstAUI TGTACA 2 cut(s) 799, 2231
BstBI TTCGAA 1 cut(s) 1898
BstC8I GCNNGC 7 cut(s) 364, 395, 869, 1202, 1690, 2109, 2474
BstF5I GGATG 9 cut(s) 289, 466, 613, 689, 727, 928, 1091, 1321, 1519
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 7 cut(s) 123, 252, 941, 1129, 1193, 2116, 2413
BstMAI GTCTC 4 cut(s) 1332, 1609, 1632, 2362
BstMBI GATC 7 cut(s) 120, 249, 938, 1126, 1190, 2113, 2410
BstMWI GCNNNNNNNGC 4 cut(s) 1558, 1741, 2218, 2473
BstNI CCWGG 3 cut(s) 678, 830, 920
BstNSI RCATGY 2 cut(s) 1561, 2439
BstSCI CCNGG 4 cut(s) 676, 828, 918, 2251
BstSFI CTRYAG 2 cut(s) 858, 872
BstSLI GKGCMC 1 cut(s) 829
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 6 cut(s) 402, 858, 886, 1100, 1216, 2199
BstV2I GAAGAC 4 cut(s) 748, 1838, 2190, 2334
BstX2I RGATCY 2 cut(s) 1126, 2410
BstXI CCANNNNNNTGG 1 cut(s) 2021
BstYI RGATCY 2 cut(s) 1126, 2410
Bsu15I ATCGAT 1 cut(s) 656
Bsu36I CCTNAGG 1 cut(s) 1096
BsuI GTATCC 1 cut(s) 1968
BsuRI GGCC 3 cut(s) 38, 84, 2013
BsuTUI ATCGAT 1 cut(s) 656
BtgZI GCGATG 1 cut(s) 1533
BtsCI GGATG 9 cut(s) 289, 466, 613, 689, 727, 928, 1091, 1321, 1519
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 5 cut(s) 117, 245, 1530, 2152, 2378
BveI ACCTGC 2 cut(s) 357, 1944
Cac8I GCNNGC 7 cut(s) 364, 395, 869, 1202, 1690, 2109, 2474
CaiI CAGNNNCTG 2 cut(s) 427, 1391
CfoI GCGC 1 cut(s) 17
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 4 cut(s) 37, 832, 916, 1862
ClaI ATCGAT 1 cut(s) 656
CseI GACGC 1 cut(s) 163
Csp6I GTAC 7 cut(s) 385, 800, 1232, 1259, 1493, 1841, 2232
CviAII CATG 8 cut(s) 815, 1085, 1274, 1553, 1558, 1999, 2365, 2436
CviQI GTAC 7 cut(s) 385, 800, 1232, 1259, 1493, 1841, 2232
DpnI GATC 7 cut(s) 122, 251, 940, 1128, 1192, 2115, 2412
DpnII GATC 7 cut(s) 120, 249, 938, 1126, 1190, 2113, 2410
DrdI GACNNNNNNGTC 1 cut(s) 1006
DriI GACNNNNNGTC 1 cut(s) 1441
DseDI GACNNNNNNGTC 1 cut(s) 1006
EaeI YGGCCR 2 cut(s) 82, 2011
Eam1104I CTCTTC 2 cut(s) 1314, 1710
Eam1105I GACNNNNNGTC 1 cut(s) 1441
EarI CTCTTC 2 cut(s) 1314, 1710
Ecl136II GAGCTC 2 cut(s) 30, 1110
Eco130I CCWWGG 2 cut(s) 1354, 2014
Eco24I GRGCYC 2 cut(s) 32, 1112
Eco32I GATATC 2 cut(s) 217, 2518
Eco47I GGWCC 3 cut(s) 832, 916, 1862
Eco53kI GAGCTC 2 cut(s) 30, 1110
Eco57I CTGAAG 8 cut(s) 404, 905, 1115, 1389, 1850, 2156, 2202, 2346
Eco81I CCTNAGG 1 cut(s) 1096
Eco88I CYCGRG 1 cut(s) 2377
EcoICRI GAGCTC 2 cut(s) 30, 1110
EcoO109I RGGNCCY 1 cut(s) 832
EcoRI GAATTC 2 cut(s) 404, 944
EcoRII CCWGG 3 cut(s) 676, 828, 918
EcoRV GATATC 2 cut(s) 217, 2518
EcoT14I CCWWGG 2 cut(s) 1354, 2014
EcoT38I GRGCYC 2 cut(s) 32, 1112
ErhI CCWWGG 2 cut(s) 1354, 2014
FaeI CATG 8 cut(s) 818, 1088, 1277, 1556, 1561, 2002, 2368, 2439
FalI AAGNNNNNCTT 4 cut(s) 770, 802, 997, 1029
FaqI GGGAC 1 cut(s) 818
FatI CATG 8 cut(s) 814, 1084, 1273, 1552, 1557, 1998, 2364, 2435
FauI CCCGC 2 cut(s) 553, 1193
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 9 cut(s) 36, 85, 93, 391, 872, 875, 1089, 1205, 2213
FokI GGATG 9 cut(s) 296, 473, 620, 696, 734, 935, 1078, 1328, 1526
FriOI GRGCYC 2 cut(s) 32, 1112
Fsp4HI GCNGC 9 cut(s) 36, 85, 93, 391, 872, 875, 1089, 1205, 2213
FspBI CTAG 7 cut(s) 302, 1245, 1838, 1889, 1925, 2198, 2539
GlaI GCGC 1 cut(s) 16
GluI GCNGC 9 cut(s) 36, 85, 93, 391, 872, 875, 1089, 1205, 2213
GsuI CTGGAG 2 cut(s) 90, 699
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 3 cut(s) 38, 84, 2013
HapII CCGG 2 cut(s) 75, 2253
HgaI GACGC 1 cut(s) 163
HhaI GCGC 1 cut(s) 17
Hin1II CATG 8 cut(s) 818, 1088, 1277, 1556, 1561, 2002, 2368, 2439
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HindIII AAGCTT 2 cut(s) 635, 1300
HinfI GANTC 8 cut(s) 164, 233, 307, 1265, 1380, 1834, 1895, 1917
HpaII CCGG 2 cut(s) 75, 2253
HphI GGTGA 7 cut(s) 89, 496, 673, 1009, 1073, 1112, 2143
Hpy166II GTNNAC 1 cut(s) 1453
Hpy8I GTNNAC 1 cut(s) 1453
Hpy99I CGWCG 2 cut(s) 53, 56
HpyAV CCTTC 6 cut(s) 175, 268, 463, 512, 1953, 2406
HpyCH4III ACNGT 7 cut(s) 598, 769, 1180, 1393, 1968, 2030, 2373
HpyCH4IV ACGT 1 cut(s) 1150
HpyF10VI GCNNNNNNNGC 4 cut(s) 1558, 1741, 2218, 2473
HpySE526I ACGT 1 cut(s) 1150
Hsp92II CATG 8 cut(s) 818, 1088, 1277, 1556, 1561, 2002, 2368, 2439
HspAI GCGC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 7 cut(s) 120, 249, 938, 1126, 1190, 2113, 2410
LmnI GCTCC 8 cut(s) 50, 68, 71, 298, 679, 1107, 2464, 2538
Lsp1109I GCAGC 6 cut(s) 402, 858, 886, 1100, 1216, 2199
LweI GCATC 4 cut(s) 712, 1054, 1100, 1297
MaeI CTAG 7 cut(s) 302, 1245, 1838, 1889, 1925, 2198, 2539
MaeII ACGT 1 cut(s) 1150
MalI GATC 7 cut(s) 122, 251, 940, 1128, 1192, 2115, 2412
MboI GATC 7 cut(s) 120, 249, 938, 1126, 1190, 2113, 2410
MflI RGATCY 2 cut(s) 1126, 2410
MhlI GDGCHC 5 cut(s) 32, 356, 829, 1112, 2378
MlsI TGGCCA 1 cut(s) 2013
MluNI TGGCCA 1 cut(s) 2013
MlyI GAGTC 3 cut(s) 227, 1274, 1828
MmeI TCCRAC 4 cut(s) 580, 611, 1165, 1603
Mox20I TGGCCA 1 cut(s) 2013
MroXI GAANNNNTTC 2 cut(s) 948, 1128
MscI TGGCCA 1 cut(s) 2013
MseI TTAA 7 cut(s) 171, 971, 1485, 1949, 2279, 2313, 2423
MslI CAYNNNNRTG 2 cut(s) 1403, 1729
Msp20I TGGCCA 1 cut(s) 2013
MspA1I CMGCKG 2 cut(s) 35, 68
MspCI CTTAAG 1 cut(s) 2312
MspI CCGG 2 cut(s) 75, 2253
MspR9I CCNGG 4 cut(s) 678, 830, 920, 2253
Mva1269I GAATGC 1 cut(s) 1346
MvaI CCWGG 3 cut(s) 678, 830, 920
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 4 cut(s) 1558, 1741, 2218, 2473
NciI CCSGG 1 cut(s) 2253
NdeII GATC 7 cut(s) 120, 249, 938, 1126, 1190, 2113, 2410
NlaIII CATG 8 cut(s) 818, 1088, 1277, 1556, 1561, 2002, 2368, 2439
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 7 cut(s) 414, 529, 997, 2120, 2149, 2431, 2446
NspI RCATGY 2 cut(s) 1561, 2439
NspV TTCGAA 1 cut(s) 1898
PaeR7I CTCGAG 1 cut(s) 2377
PciI ACATGT 1 cut(s) 2435
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 1346
PdmI GAANNNNTTC 2 cut(s) 948, 1128
PfeI GAWTC 5 cut(s) 164, 307, 1380, 1895, 1917
PflMI CCANNNNNTGG 1 cut(s) 1025
PfoI TCCNGGA 2 cut(s) 676, 918
PkrI GCNGC 9 cut(s) 37, 86, 94, 392, 873, 876, 1090, 1206, 2214
PleI GAGTC 3 cut(s) 227, 1273, 1828
PpsI GAGTC 3 cut(s) 227, 1273, 1828
PpuMI RGGWCCY 1 cut(s) 832
PscI ACATGT 1 cut(s) 2435
Psp124BI GAGCTC 2 cut(s) 32, 1112
Psp1406I AACGTT 1 cut(s) 1150
Psp5II RGGWCCY 1 cut(s) 832
Psp6I CCWGG 3 cut(s) 676, 828, 918
PspGI CCWGG 3 cut(s) 676, 828, 918
PspPI GGNCC 4 cut(s) 37, 832, 916, 1862
PspPPI RGGWCCY 1 cut(s) 832
PstI CTGCAG 2 cut(s) 862, 876
PstNI CAGNNNCTG 2 cut(s) 427, 1391
PsuI RGATCY 2 cut(s) 1126, 2410
RsaI GTAC 7 cut(s) 386, 801, 1233, 1260, 1494, 1842, 2233
RsaNI GTAC 7 cut(s) 385, 800, 1232, 1259, 1493, 1841, 2232
RseI CAYNNNNRTG 2 cut(s) 1403, 1729
SacI GAGCTC 2 cut(s) 32, 1112
SaqAI TTAA 7 cut(s) 171, 971, 1485, 1949, 2279, 2313, 2423
SatI GCNGC 9 cut(s) 36, 85, 93, 391, 872, 875, 1089, 1205, 2213
Sau3AI GATC 7 cut(s) 120, 249, 938, 1126, 1190, 2113, 2410
Sau96I GGNCC 4 cut(s) 37, 832, 916, 1862
ScaI AGTACT 2 cut(s) 1233, 1842
SchI GAGTC 3 cut(s) 227, 1274, 1828
ScrFI CCNGG 4 cut(s) 678, 830, 920, 2253
SduI GDGCHC 5 cut(s) 32, 356, 829, 1112, 2378
SfaNI GCATC 4 cut(s) 712, 1054, 1100, 1297
SfcI CTRYAG 2 cut(s) 858, 872
Sfr274I CTCGAG 1 cut(s) 2377
SfuI TTCGAA 1 cut(s) 1898
SinI GGWCC 3 cut(s) 832, 916, 1862
SlaI CTCGAG 1 cut(s) 2377
SmiMI CAYNNNNRTG 2 cut(s) 1403, 1729
SmlI CTYRAG 5 cut(s) 953, 1262, 2270, 2312, 2377
SmoI CTYRAG 5 cut(s) 953, 1262, 2270, 2312, 2377
SpeI ACTAGT 1 cut(s) 1888
SsiI CCGC 7 cut(s) 35, 66, 85, 93, 346, 560, 1200
SspI AATATT 1 cut(s) 2077
SspMI CTAG 7 cut(s) 302, 1245, 1838, 1889, 1925, 2198, 2539
SstI GAGCTC 2 cut(s) 32, 1112
StyD4I CCNGG 4 cut(s) 676, 828, 918, 2251
StyI CCWWGG 2 cut(s) 1354, 2014
TaaI ACNGT 7 cut(s) 598, 769, 1180, 1393, 1968, 2030, 2373
TaiI ACGT 1 cut(s) 1153
TaqI TCGA 8 cut(s) 26, 356, 495, 656, 1898, 2258, 2297, 2378
TaqII GACCGA 1 cut(s) 1850
TatI WGTACW 6 cut(s) 799, 1231, 1258, 1492, 1840, 2231
TauI GCSGC 3 cut(s) 38, 87, 95
TfiI GAWTC 5 cut(s) 164, 307, 1380, 1895, 1917
Tru1I TTAA 7 cut(s) 171, 971, 1485, 1949, 2279, 2313, 2423
Tru9I TTAA 7 cut(s) 171, 971, 1485, 1949, 2279, 2313, 2423
TscAI CASTG 5 cut(s) 117, 252, 1537, 2152, 2378
TseFI GTSAC 7 cut(s) 414, 529, 997, 2120, 2149, 2431, 2446
TseI GCWGC 6 cut(s) 390, 871, 874, 1088, 1204, 2212
Tsp45I GTSAC 7 cut(s) 414, 529, 997, 2120, 2149, 2431, 2446
TspGWI ACGGA 2 cut(s) 37, 663
TspRI CASTG 5 cut(s) 117, 252, 1537, 2152, 2378
Van91I CCANNNNNTGG 1 cut(s) 1025
Vha464I CTTAAG 1 cut(s) 2312
VpaK11BI GGWCC 3 cut(s) 832, 916, 1862
XapI RAATTY 5 cut(s) 404, 740, 944, 1932, 2203
XbaI TCTAGA 1 cut(s) 1244
XceI RCATGY 2 cut(s) 1561, 2439
XcmI CCANNNNNNNNNTGG 2 cut(s) 147, 1215
XhoI CTCGAG 1 cut(s) 2377
XmnI GAANNNNTTC 2 cut(s) 948, 1128
XspI CTAG 7 cut(s) 302, 1245, 1838, 1889, 1925, 2198, 2539
ZrmI AGTACT 2 cut(s) 1233, 1842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.