FvH4_6g50100

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000039
Physical Location & Seq
Reverse (-)
37342661 .. 37351274
8614 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50100.t2

Sequence Viewer

Length: 2625 bp
ATGGCTGTGGATTTAGTGGCAGGGAAAGCATTCGAGGCACTCTATGCCGGTGTTGAAGAGGTTATTCGCAAGAATCGGATATTCTCAGAACTCTGCAAGGACATCAAATACAGGCTCGACTGTTTAGCACCACTGATTGACGAGATGGCGAAATATAATCAAAAATTGGATCGCACAAAGGAGTTGGAAGCTTTAGAAAGACTCATAAAGCGGGGCATAGAGCTCGTCGGCAAATGCTCTAAGCTTCGTAAGTGGAACATCTACAAGAAGTGGAAGTACGCCAGCGAGTTTCTTGAATTCAAAGAATCACTCCAAAACGAGTTAGATGTGCTCAAGGTGCAGGTAGCAAGAGATGTGAAGAAGACTGAAGTTGGCGTAGAGAATATCCAGGTCGTGGTTCAAAACATACAGGAGGAGGTTTCTATGCTTGTGAAGCAAAATGGATCCGTAGAAATCAAAGCTTTAAGTGCAGTACTACCTGAACTTCCAGCGCTAATAGTCGGATTGGAAGTTCCTATAGAAGAATTGAAGGTGCAGCTTCTTAAGGATGAGGTATCCATTCTCATCGTGACTGCTCCTGGAGGATGTGGCAAGACAACTTTGGCAACAAAGTTTTTTCAAGATCAAGACATCATAGATAAATTCCAGAAGAACATCTTCTTCGTCACTTTTTCTAAAAAGCCCAGCCTGGAGCTTATTATGAAAGGGTTGTATGAACAAAAGGGTTTTGAGGTACCTACTCTCCAGAATGAAGCAACTCCAGTCAAATGGCTGAAAAAAGTTGAGATGGAAAAAGGTGATGATCCTTTATTGTTGGTCTTGGATGATGTTTGGTCAGGATCAGAATCTCTTCTTGAGAAGTTTGAGTATAGCATGTCAAATTACAAGATTTTAGTGACATCGAGGTCTGAATTTCCGAGATTTGGTTCCTCATATCATCTAAAACTACTGGATAGTGACAATGCTATGGAACTTTTTTGTCATACAGCATCCTTGGGAGACAAGAGCTCTTATATTCCAAAAGATCTTTTAGAAGAGATAGTAAAACATTGTAATGGATTTCCACTGGCCATTACAGTGGCTGGAAGATCACTTTGTGGGCAACCTGAACAAATGTGGCGAAGTAGAGTAGATGAATGGTCGGATGGTTCTTCCATTCTTGATTTCGAGACGGAACTGCTTCTTCAGCTCCAAAGCAGCATAGATGTCTTGGATAATGCAATGCCTAAGGTCAAAGAATGTTTTTTAGACCTAGGTGCTTTTCCTGAAGATGTTGAAATCCCAGTTGCCGCTCTCATTGATATGTGGGCAGAGTTGTATGATCTAAAGAAAGAGACGTCATGCATTGCCAAACTCTGTGAACTCAACAATCGCAGTCTAGCTCTTTTACAAGAATCAGAATGGTCTGCGGATTGGGAATTGGCACAGGAGTCAATAGATTACAGTAAGTACGTTGTTAAGCAGCATGATTTGCTCAGAGAACTTGCTATATACAACTCGAAGCGGGATCCGATTGAGCATAGAGAAAGACTGATTATAGAGATATGTGGAAACAATTTTCCAAAATGGTGGAGAGAACAAAAATATCCATACATGAATGCTCGCCTTGTTTCAATCACAACTGATGAAGAGTTCTCAGTGAAATTGCCCAACATGCAATTACCAGAGGCAGAGGTCCTAGTCTTGAATTTTCAATCCGACACGTATGCCTTGCCCGAATTTGTGGAGAGAATGGATAAACTAAAGATGCTTCTTGTCCTAAATTATTCTCTATATTGTGATGAATTGGATAATTTTCAACTACTGGGCTCCTTAACAAATCTTACCTGTGTCAGATTAGACGGCATCGATATTCGTTGCATAACCAAGAGCCCAGTACAAATGAAAAATCTACACAAGTTATATTTGAGGAATGGTAGCTATCTCGGTGAAGATCCTGTCAAATTATCATATGTATTTCCGAATCTGAAGGAAATGGTCATTGACTCCTACGGTATGAGTGAATGGCCTGAAGAGTTCTGCGAGTTGATTCATCTAACAAAGCTCAAAATCATTAAATGTCCCGATTTATCAGACTTGCCTGAAGCGATTGGGAATTTGGTCAGTTTAGAAGTGTTGGAGCTTGAAGCCTGTCCTCTTTCAGAGCTTCCTGACTCAATCGGGAATCTTAAGAAATTAAAATCTCTGAAGATTTTTTGTTCGGTAATTGAAAAGTTGCCTGAACACATGGGTGAGCTCAAGTGTCTAACACATCTCGGCTTAAACTGTTGCCATTTTCTATCTGCCTTGCCTGAAACGATTGGAAATCTGATCAATTTAGAAGTACTGAGTCTAAGGTACTGTTTTGAGTTGTCAGAGCTGCCGCAGTCGATCAGAAATCTTAAAAAGTTGAACATTCTTGACATCCACGCTTGCTGCAAAATGATGGAGTTGCCTGAACATATTGGTGAATTGAAGAGTTTAACGAAGCTGAAAATGGTTGATTGTTATGCATTGAGAAAATTGCCTGAATCAGTTTCAGATCTTGAGCAGTTAGAGGAAGTGATATGCGAAGACTGGGACGCAGAACATCTATGGACACCCTTGTTACCCACTCTCAAAAACCTTCACATCGTGGCGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

875

Amino Acids

100.09

Weight (kDa)

5.05

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 59 - 177 8e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 223 - 381 3.3e-15 NB-ARC domain
LRR_14 PF23598 723 - 807 6e-13 Leucine-rich repeat region
LRR_14 PF23598 792 - 921 4e-15 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 904
AatII GACGTC 1 cut(s) 1340
Acc36I ACCTGC 1 cut(s) 331
Acc65I GGTACC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 733
AccB7I CCANNNNNTGG 1 cut(s) 394
AccBSI CCGCTC 1 cut(s) 1292
AccII CGCG 1 cut(s) 2620
AciI CCGC 6 cut(s) 211, 1290, 1409, 1504, 2363, 2620
AclWI GGATC 8 cut(s) 177, 438, 451, 797, 847, 1502, 1515, 1928
AcoI YGGCCR 1 cut(s) 1068
AcsI RAATTY 6 cut(s) 296, 641, 911, 1687, 1718, 2095
AcuI CTGAAG 7 cut(s) 387, 1169, 1287, 1988, 2031, 2103, 2207
AcyI GRCGYC 1 cut(s) 1337
AdeI CACNNNGTG 2 cut(s) 1097, 2614
AfaI GTAC 7 cut(s) 278, 474, 735, 1451, 1878, 2325, 2339
AfeI AGCGCT 1 cut(s) 492
AfiI CCNNNNNNNGG 2 cut(s) 394, 923
AflII CTTAAG 2 cut(s) 542, 2168
AflIII ACRYGT 1 cut(s) 1701
AjnI CCWGG 3 cut(s) 387, 577, 687
AleI CACNNNNGTG 1 cut(s) 2229
AloI GAACNNNNNNTCC 2 cut(s) 495, 527
Alw21I GWGCWC 4 cut(s) 225, 333, 1010, 2238
Alw26I GTCTC 3 cut(s) 993, 1163, 1328
AlwI GGATC 8 cut(s) 177, 438, 451, 797, 847, 1502, 1515, 1928
AlwNI CAGNNNCTG 1 cut(s) 1082
Aor51HI AGCGCT 1 cut(s) 492
AoxI GGCC 2 cut(s) 1068, 2006
ApeKI GCWGC 5 cut(s) 535, 1197, 1462, 2359, 2415
ApoI RAATTY 6 cut(s) 296, 641, 911, 1687, 1718, 2095
Asp700I GAANNNNTTC 4 cut(s) 29, 656, 849, 1179
Asp718I GGTACC 1 cut(s) 733
AspA2I CCTAGG 1 cut(s) 1252
AspLEI GCGC 2 cut(s) 493, 2620
AspS9I GGNCC 1 cut(s) 1675
AsuHPI GGTGA 4 cut(s) 809, 1940, 2243, 2459
AvaII GGWCC 1 cut(s) 1675
AvrII CCTAGG 1 cut(s) 1252
AxyI CCTNAGG 1 cut(s) 1227
BalI TGGCCA 1 cut(s) 1070
BamHI GGATCC 2 cut(s) 443, 1507
BanI GGYRCC 1 cut(s) 733
BanII GRGCYC 5 cut(s) 225, 1010, 1811, 1874, 2238
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbsI GAAGAC 2 cut(s) 368, 2559
Bbv12I GWGCWC 4 cut(s) 225, 333, 1010, 2238
BbvI GCAGC 5 cut(s) 547, 1209, 1474, 2346, 2402
BccI CCATC 4 cut(s) 139, 781, 1139, 2419
BceAI ACGGC 1 cut(s) 1858
BcgI CGANNNNNNTGC 4 cut(s) 205, 239, 1688, 1722
BciT130I CCWGG 3 cut(s) 389, 579, 689
BciVI GTATCC 1 cut(s) 565
BclI TGATCA 1 cut(s) 2310
BcoDI GTCTC 3 cut(s) 993, 1163, 1328
BfaI CTAG 3 cut(s) 1253, 1379, 1679
BfmI CTRYAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 494
BfrI CTTAAG 2 cut(s) 542, 2168
BfuAI ACCTGC 1 cut(s) 331
BfuI GTATCC 1 cut(s) 565
BglII AGATCT 2 cut(s) 1024, 2521
BisI GCNGC 7 cut(s) 536, 1198, 1290, 1463, 2360, 2363, 2416
BlnI CCTAGG 1 cut(s) 1252
BlsI GCNGC 7 cut(s) 537, 1199, 1291, 1464, 2361, 2364, 2417
BmcAI AGTACT 2 cut(s) 474, 2325
Bme1390I CCNGG 3 cut(s) 389, 579, 689
Bme18I GGWCC 1 cut(s) 1675
BmgT120I GGNCC 1 cut(s) 1675
BmiI GGNNCC 5 cut(s) 445, 735, 928, 1509, 1810
BmrFI CCNGG 3 cut(s) 389, 579, 689
BmrI ACTGGG 4 cut(s) 1277, 1814, 1868, 2566
BmsI GCATC 3 cut(s) 998, 1737, 1854
BmuI ACTGGG 4 cut(s) 1277, 1814, 1868, 2566
BpiI GAAGAC 2 cut(s) 368, 2559
BpmI CTGGAG 4 cut(s) 600, 710, 728, 744
BpuEI CTTGAG 4 cut(s) 317, 875, 2222, 2546
Bsa29I ATCGAT 1 cut(s) 1848
BsaAI YACGTR 1 cut(s) 1704
BsaBI GATNNNNATC 1 cut(s) 844
BsaHI GRCGYC 1 cut(s) 1337
BsaJI CCNNGG 2 cut(s) 993, 1252
BsaXI ACNNNNNCTCC 2 cut(s) 726, 756
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 923
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 7 cut(s) 761, 954, 1071, 1283, 1809, 1874, 2561
Bse21I CCTNAGG 1 cut(s) 1227
Bse3DI GCAATG 2 cut(s) 1227, 1344
Bse8I GATNNNNATC 1 cut(s) 844
BseBI CCWGG 3 cut(s) 389, 579, 689
BseCI ATCGAT 1 cut(s) 1848
BseDI CCNNGG 2 cut(s) 993, 1252
BseGI GGATG 6 cut(s) 553, 590, 829, 989, 1150, 2403
BseJI GATNNNNATC 1 cut(s) 844
BseLI CCNNNNNNNGG 2 cut(s) 394, 923
BseMI GCAATG 2 cut(s) 1227, 1344
BseMII CTCAG 4 cut(s) 99, 1489, 1650, 2318
BseNI ACTGG 7 cut(s) 761, 954, 1071, 1283, 1809, 1874, 2561
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 5 cut(s) 547, 1209, 1474, 2346, 2402
BseYI CCCAGC 1 cut(s) 683
BsgI GTGCAG 3 cut(s) 359, 489, 554
Bsh1236I CGCG 1 cut(s) 2620
BshFI GGCC 2 cut(s) 1070, 2008
BshNI GGYRCC 1 cut(s) 733
BshVI ATCGAT 1 cut(s) 1848
BsiHKAI GWGCWC 4 cut(s) 225, 333, 1010, 2238
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 2 cut(s) 2046, 2573
BslI CCNNNNNNNGG 2 cut(s) 394, 923
BsmAI GTCTC 3 cut(s) 993, 1163, 1328
BsmBI CGTCTC 2 cut(s) 1163, 1328
BsmFI GGGAC 2 cut(s) 2046, 2573
BsmI GAATGC 2 cut(s) 29, 1603
BsnI GGCC 2 cut(s) 1070, 2008
Bsp1286I GDGCHC 6 cut(s) 225, 333, 1010, 1811, 1874, 2238
BspACI CCGC 6 cut(s) 211, 1290, 1409, 1504, 2363, 2620
BspANI GGCC 2 cut(s) 1070, 2008
BspCNI CTCAG 4 cut(s) 98, 1488, 1649, 2319
BspDI ATCGAT 1 cut(s) 1848
BspFNI CGCG 1 cut(s) 2620
BspLI GGNNCC 5 cut(s) 445, 735, 928, 1509, 1810
BspMI ACCTGC 1 cut(s) 331
BspPI GGATC 8 cut(s) 177, 438, 451, 797, 847, 1502, 1515, 1928
BspT107I GGYRCC 1 cut(s) 733
BspTI CTTAAG 2 cut(s) 542, 2168
BsrBI CCGCTC 1 cut(s) 1292
BsrDI GCAATG 2 cut(s) 1227, 1344
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 7 cut(s) 761, 954, 1071, 1283, 1809, 1874, 2561
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 993, 1252
BssNI GRCGYC 1 cut(s) 1337
BssT1I CCWWGG 2 cut(s) 993, 1252
Bst2UI CCWGG 3 cut(s) 389, 579, 689
Bst4CI ACNGT 6 cut(s) 122, 1078, 1445, 1994, 2267, 2342
Bst6I CTCTTC 6 cut(s) 51, 855, 1029, 1623, 2007, 2450
BstACI GRCGYC 1 cut(s) 1337
BstAFI CTTAAG 2 cut(s) 542, 2168
BstAPI GCANNNNNTGC 2 cut(s) 44, 1471
BstBAI YACGTR 1 cut(s) 1704
BstC8I GCNNGC 3 cut(s) 283, 1603, 2413
BstDEI CTNAG 7 cut(s) 85, 240, 1227, 1475, 1636, 2327, 2333
BstF5I GGATG 6 cut(s) 553, 590, 829, 989, 1150, 2403
BstFNI CGCG 1 cut(s) 2620
BstH2I RGCGCY 1 cut(s) 494
BstHHI GCGC 2 cut(s) 493, 2620
BstMAI GTCTC 3 cut(s) 993, 1163, 1328
BstMWI GCNNNNNNNGC 9 cut(s) 26, 35, 44, 337, 433, 467, 1186, 1471, 1654
BstNI CCWGG 3 cut(s) 389, 579, 689
BstNSI RCATGY 2 cut(s) 877, 1657
BstSCI CCNGG 3 cut(s) 387, 577, 687
BstSFI CTRYAG 1 cut(s) 516
BstUI CGCG 1 cut(s) 2620
BstV1I GCAGC 5 cut(s) 547, 1209, 1474, 2346, 2402
BstV2I GAAGAC 2 cut(s) 368, 2559
BstX2I RGATCY 5 cut(s) 443, 1024, 1507, 1933, 2521
BstXI CCANNNNNNTGG 3 cut(s) 768, 1078, 1569
BstYI RGATCY 5 cut(s) 443, 1024, 1507, 1933, 2521
Bsu15I ATCGAT 1 cut(s) 1848
Bsu36I CCTNAGG 1 cut(s) 1227
BsuI GTATCC 1 cut(s) 565
BsuRI GGCC 2 cut(s) 1070, 2008
BsuTUI ATCGAT 1 cut(s) 1848
BtsCI GGATG 6 cut(s) 553, 590, 829, 989, 1150, 2403
BtsIMutI CAGTG 4 cut(s) 131, 1064, 1083, 1644
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 3 cut(s) 283, 1603, 2413
CaiI CAGNNNCTG 1 cut(s) 1082
CfoI GCGC 2 cut(s) 493, 2620
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 1675
ClaI ATCGAT 1 cut(s) 1848
CseI GACGC 1 cut(s) 2570
Csp6I GTAC 7 cut(s) 277, 473, 734, 1450, 1877, 2324, 2338
CviAII CATG 6 cut(s) 874, 1341, 1466, 1594, 1654, 2227
CviQI GTAC 7 cut(s) 277, 473, 734, 1450, 1877, 2324, 2338
DdeI CTNAG 7 cut(s) 85, 240, 1227, 1475, 1636, 2327, 2333
DraIII CACNNNGTG 2 cut(s) 1097, 2614
DrdI GACNNNNNNGTC 1 cut(s) 904
DseDI GACNNNNNNGTC 1 cut(s) 904
EaeI YGGCCR 1 cut(s) 1068
Eam1104I CTCTTC 6 cut(s) 51, 855, 1029, 1623, 2007, 2450
EarI CTCTTC 6 cut(s) 51, 855, 1029, 1623, 2007, 2450
Ecl136II GAGCTC 3 cut(s) 223, 1008, 2236
Eco130I CCWWGG 2 cut(s) 993, 1252
Eco24I GRGCYC 5 cut(s) 225, 1010, 1811, 1874, 2238
Eco47I GGWCC 1 cut(s) 1675
Eco47III AGCGCT 1 cut(s) 492
Eco53kI GAGCTC 3 cut(s) 223, 1008, 2236
Eco57I CTGAAG 7 cut(s) 387, 1169, 1287, 1988, 2031, 2103, 2207
Eco81I CCTNAGG 1 cut(s) 1227
EcoICRI GAGCTC 3 cut(s) 223, 1008, 2236
EcoO109I RGGNCCY 1 cut(s) 1675
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 3 cut(s) 387, 577, 687
EcoT14I CCWWGG 2 cut(s) 993, 1252
EcoT22I ATGCAT 2 cut(s) 1346, 2494
EcoT38I GRGCYC 5 cut(s) 225, 1010, 1811, 1874, 2238
ErhI CCWWGG 2 cut(s) 993, 1252
Esp3I CGTCTC 2 cut(s) 1163, 1328
FaeI CATG 6 cut(s) 877, 1344, 1469, 1597, 1657, 2230
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FaqI GGGAC 2 cut(s) 2046, 2573
FatI CATG 6 cut(s) 873, 1340, 1465, 1593, 1653, 2226
FauI CCCGC 2 cut(s) 204, 1497
FauNDI CATATG 1 cut(s) 1951
FbaI TGATCA 1 cut(s) 2310
Fnu4HI GCNGC 7 cut(s) 536, 1198, 1290, 1463, 2360, 2363, 2416
FokI GGATG 6 cut(s) 560, 597, 836, 976, 1157, 2390
FriOI GRGCYC 5 cut(s) 225, 1010, 1811, 1874, 2238
Fsp4HI GCNGC 7 cut(s) 536, 1198, 1290, 1463, 2360, 2363, 2416
FspBI CTAG 3 cut(s) 1253, 1379, 1679
GlaI GCGC 2 cut(s) 492, 2619
GluI GCNGC 7 cut(s) 536, 1198, 1290, 1463, 2360, 2363, 2416
GsaI CCCAGC 1 cut(s) 687
GsuI CTGGAG 4 cut(s) 600, 710, 728, 744
HaeII RGCGCY 1 cut(s) 494
HaeIII GGCC 2 cut(s) 1070, 2008
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 2570
HhaI GCGC 2 cut(s) 493, 2620
Hin1I GRCGYC 1 cut(s) 1337
Hin1II CATG 6 cut(s) 877, 1344, 1469, 1597, 1657, 2230
Hin6I GCGC 2 cut(s) 491, 2618
HinP1I GCGC 2 cut(s) 491, 2618
HindIII AAGCTT 3 cut(s) 189, 242, 459
HpaII CCGG 1 cut(s) 48
HphI GGTGA 4 cut(s) 809, 1940, 2243, 2459
Hpy166II GTNNAC 1 cut(s) 1361
Hpy8I GTNNAC 1 cut(s) 1361
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 3 cut(s) 523, 1963, 2615
HpyCH4III ACNGT 6 cut(s) 122, 1078, 1445, 1994, 2267, 2342
HpyCH4IV ACGT 3 cut(s) 1337, 1452, 1703
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 35, 44, 337, 433, 467, 1186, 1471, 1654
HpyF3I CTNAG 7 cut(s) 85, 240, 1227, 1475, 1636, 2327, 2333
HpySE526I ACGT 3 cut(s) 1337, 1452, 1703
Hsp92I GRCGYC 1 cut(s) 1337
Hsp92II CATG 6 cut(s) 877, 1344, 1469, 1597, 1657, 2230
HspAI GCGC 2 cut(s) 491, 2618
KpnI GGTACC 1 cut(s) 737
Ksp22I TGATCA 1 cut(s) 2310
LmnI GCTCC 5 cut(s) 580, 691, 1194, 1814, 2119
Lsp1109I GCAGC 5 cut(s) 547, 1209, 1474, 2346, 2402
LweI GCATC 3 cut(s) 998, 1737, 1854
MaeI CTAG 3 cut(s) 1253, 1379, 1679
MaeII ACGT 3 cut(s) 1337, 1452, 1703
MaeIII GTNAC 5 cut(s) 568, 664, 895, 956, 2586
MbiI CCGCTC 1 cut(s) 1292
MflI RGATCY 5 cut(s) 443, 1024, 1507, 1933, 2521
MhlI GDGCHC 6 cut(s) 225, 333, 1010, 1811, 1874, 2238
MlsI TGGCCA 1 cut(s) 1070
MluNI TGGCCA 1 cut(s) 1070
MlyI GAGTC 5 cut(s) 195, 1439, 1979, 2147, 2338
MmeI TCCRAC 5 cut(s) 165, 481, 1122, 1722, 2097
Mox20I TGGCCA 1 cut(s) 1070
Mph1103I ATGCAT 2 cut(s) 1346, 2494
MroXI GAANNNNTTC 4 cut(s) 29, 656, 849, 1179
MscI TGGCCA 1 cut(s) 1070
MslI CAYNNNNRTG 5 cut(s) 1053, 1076, 1301, 2229, 2445
Msp20I TGGCCA 1 cut(s) 1070
MspCI CTTAAG 2 cut(s) 542, 2168
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 3 cut(s) 389, 579, 689
Mva1269I GAATGC 2 cut(s) 29, 1603
MvaI CCWGG 3 cut(s) 389, 579, 689
MvnI CGCG 1 cut(s) 2620
MwoI GCNNNNNNNGC 9 cut(s) 26, 35, 44, 337, 433, 467, 1186, 1471, 1654
NdeI CATATG 1 cut(s) 1951
NlaIII CATG 6 cut(s) 877, 1344, 1469, 1597, 1657, 2230
NlaIV GGNNCC 5 cut(s) 445, 735, 928, 1509, 1810
NmeAIII GCCGAG 1 cut(s) 2235
NmuCI GTSAC 4 cut(s) 568, 664, 895, 956
NsiI ATGCAT 2 cut(s) 1346, 2494
NspI RCATGY 2 cut(s) 877, 1657
OliI CACNNNNGTG 1 cut(s) 2229
PctI GAATGC 2 cut(s) 29, 1603
PdmI GAANNNNTTC 4 cut(s) 29, 656, 849, 1179
PfeI GAWTC 8 cut(s) 73, 305, 845, 1394, 1963, 2029, 2164, 2510
PflMI CCANNNNNTGG 1 cut(s) 394
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 7 cut(s) 537, 1199, 1291, 1464, 2361, 2364, 2417
PleI GAGTC 5 cut(s) 195, 1438, 1979, 2147, 2337
PpsI GAGTC 5 cut(s) 195, 1438, 1979, 2147, 2337
Ppu21I YACGTR 1 cut(s) 1704
PpuMI RGGWCCY 1 cut(s) 1675
Psp124BI GAGCTC 3 cut(s) 225, 1010, 2238
Psp5II RGGWCCY 1 cut(s) 1675
Psp6I CCWGG 3 cut(s) 387, 577, 687
PspFI CCCAGC 1 cut(s) 683
PspGI CCWGG 3 cut(s) 387, 577, 687
PspN4I GGNNCC 5 cut(s) 445, 735, 928, 1509, 1810
PspPI GGNCC 1 cut(s) 1675
PspPPI RGGWCCY 1 cut(s) 1675
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 5 cut(s) 443, 1024, 1507, 1933, 2521
RsaI GTAC 7 cut(s) 278, 474, 735, 1451, 1878, 2325, 2339
RsaNI GTAC 7 cut(s) 277, 473, 734, 1450, 1877, 2324, 2338
RseI CAYNNNNRTG 5 cut(s) 1053, 1076, 1301, 2229, 2445
SacI GAGCTC 3 cut(s) 225, 1010, 2238
SatI GCNGC 7 cut(s) 536, 1198, 1290, 1463, 2360, 2363, 2416
Sau96I GGNCC 1 cut(s) 1675
ScaI AGTACT 2 cut(s) 474, 2325
SchI GAGTC 5 cut(s) 195, 1439, 1979, 2147, 2338
ScrFI CCNGG 3 cut(s) 389, 579, 689
SduI GDGCHC 6 cut(s) 225, 333, 1010, 1811, 1874, 2238
SfaNI GCATC 3 cut(s) 998, 1737, 1854
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 1 cut(s) 1675
SmiMI CAYNNNNRTG 5 cut(s) 1053, 1076, 1301, 2229, 2445
SmlI CTYRAG 6 cut(s) 332, 542, 854, 2168, 2237, 2525
SmoI CTYRAG 6 cut(s) 332, 542, 854, 2168, 2237, 2525
SsiI CCGC 6 cut(s) 211, 1290, 1409, 1504, 2363, 2620
SspMI CTAG 3 cut(s) 1253, 1379, 1679
SstI GAGCTC 3 cut(s) 225, 1010, 2238
StyD4I CCNGG 3 cut(s) 387, 577, 687
StyI CCWWGG 2 cut(s) 993, 1252
TaaI ACNGT 6 cut(s) 122, 1078, 1445, 1994, 2267, 2342
TaiI ACGT 3 cut(s) 1340, 1455, 1706
TaqI TCGA 7 cut(s) 33, 117, 902, 1167, 1499, 1848, 2369
TatI WGTACW 3 cut(s) 472, 1876, 2323
TauI GCSGC 2 cut(s) 1292, 2365
TfiI GAWTC 8 cut(s) 73, 305, 845, 1394, 1963, 2029, 2164, 2510
TscAI CASTG 4 cut(s) 138, 1071, 1083, 1644
TseFI GTSAC 4 cut(s) 568, 664, 895, 956
TseI GCWGC 5 cut(s) 535, 1197, 1462, 2359, 2415
Tsp45I GTSAC 4 cut(s) 568, 664, 895, 956
TspDTI ATGAA 9 cut(s) 716, 729, 765, 1149, 1610, 1641, 1797, 1898, 2021
TspGWI ACGGA 2 cut(s) 436, 1187
TspRI CASTG 4 cut(s) 138, 1071, 1083, 1644
Van91I CCANNNNNTGG 1 cut(s) 394
Vha464I CTTAAG 2 cut(s) 542, 2168
VpaK11BI GGWCC 1 cut(s) 1675
XapI RAATTY 6 cut(s) 296, 641, 911, 1687, 1718, 2095
XceI RCATGY 2 cut(s) 877, 1657
XmaJI CCTAGG 1 cut(s) 1252
XmnI GAANNNNTTC 4 cut(s) 29, 656, 849, 1179
XspI CTAG 3 cut(s) 1253, 1379, 1679
ZraI GACGTC 1 cut(s) 1338
ZrmI AGTACT 2 cut(s) 474, 2325
Zsp2I ATGCAT 2 cut(s) 1346, 2494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.