RLG00000017328

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000039
Physical Location & Seq
Forward (+)
15342723 .. 15363467
20745 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017328

Sequence Viewer

Length: 2433 bp
ATGGTGATAATCTGCGGAGCTGGTTTAGCGGTGACATTTTGGGCGCTGTGTGATTCTGTTAAGCAAGTGAAGGACCAGATACTGATGTTCAAACCCCAACTCGGGGATATCAAGTCCACTCTGGACTCTTTGCAGCCACTGATCAAGGAGATGGCAGAGTCTGAGAAGGAACTGGATGGTCCAGAGGAGGAACTAGAGAATCTTAGAGTGGAAATGCAGGAAGGAGTGGAGCTCATTCGCAAGTGCTCGGAAGCGAGCAGGTTCAAGAAGTATAAGTATGGCAGCAGACTTGTTGACTTGAGCAAGTCACTTCAGAGGCTGTTGGGGATATTGAAAGTGCAGGGCATCAGGGATGTGAAGAAGGCATTGGTTTCCGTGAGGAACGTTGAGAATGTGGTGCACAGGATTGAAGGGAATTGTGTGATGCAAAACAATCAGTCTGAAAAAATTGAAGGGTGGTGTGCTGTGCCTGACACTCCAACAATTACGGTTGGATTGGATTTGCCTTTGAGGGAATTGAAGATGCAGCTCTTTAAAGACGGAGTGTCGATGGTGATACTTACTGCTCCAGGAGGATGTGGGAAAACTACACTGGCAACAAAATTGTGTCATGATGCAGAAGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACCTGTACCTAATTGTAAAAGAGCTATATCATCGAACGGGTGCCCAGGTCCCCGATTTCCAAAGTGAAGTAAATGCAGTTAGCTGGCTGCAGCAATTTCTGAAGCAAACAGGTCAAAATCCTATGTTGTTGGTCCTGGATGATGTTTGGGCTGGATCAGAATTCATTCTTGAGAAGTTCGATGAATTAAAAATGACAAATTACAAGATATTGGTGACTTCAAGGTCTGCTTTTCCAAGGTTTGGTTCTCCATATTATTTGGAATGTTTGAACCGCGAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGTGATAGGAGCTCTTATATTTCAGAAGATCTTCCGAGAAAGATAGTAGAACGTTGTATGGGATTTCCACTTGCAATTAAAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTAATGGAATGGTCTAGAGGTTCTTCTGTTCTTCAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGTAGTTTAGATGCTTTGGATGCAGAGAAGCCTCTTATGAGGGAATGCTTTATAGACCTTGGTATATTTCCCGAAGATCAAAGAATCCCTACCACTGTCCTCATTGATATGTGGGCAGAATTATATGGCATACAAGAAGATATTTCGTCCATTGTGAACCTCTATGAACTTGCCTCTCGAAGTTTGGCTAATATGGTAGTCACAAGGAATGACAAGGATGGCGATGGCTATTACACTGAACACTTTGTTACACAGCATGACTTGCTTAGGGAGTTGGCTATCCAACAGATTAGTCGGGAACCACTACAACAGAGAATGAGACTTATGATTGACATAAGTGGAGACGACTTTCCTATGTGGTGGAAAGAACAAAAGTATCAATCCCTCATAGCTCGCCTTGTATCTATCTCAACAGACGAAGAGTTCTCATCTAAATGGCAAAACTTGCCACTACCAGAAGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTGGAGGAAATGAATAAACTGAAGACTCTAGTACTCACAAGTTCAGGTATCGGTCCTGCTGAGTTGAATAACTTTCAACTAGTTGCTTCGAAACCAAATCTAAAGAGAATCAGACTAGAACGAATTTCATTTCCTTCAATAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTAGCGTTGGCCAAGGTTTTGGTAACGGTTCCATACATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGTTGCCTGCCGATCTCTGTGACCTTATTCAACTGAAGATGCTCAGTGTCACCAATTCTCATAAGCTCTCTGTTTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGCTGCTGAGGCTTAGGTGTTGTACAGACTTGTCAGAGTTACCCGGCTCGATTAGGAACCTCAAGAACTTAAACTTGCTTGACATATCGAATTGCTTTAGCCTTAAGGAATTGCCTGAAGACATTGGAGAAATGTGCAGTTTAGAGAAGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCATCAGTTGTGGATCTTGAAAAGTTAATGGAAGTGACATGTGATGAGGAGACAGAAATCTTATGGGAGCCTTTCTTACCGTATCTCAGTAACATACACATAAAGGTTGTCAAAGAAGACATCAACCTGAATTGGCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

811

Amino Acids

92.24

Weight (kDa)

5.22

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 130 8.9e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 170 - 326 5.5e-16 NB-ARC domain
AAA_22 PF13401 183 - 279 1.3e-06 AAA domain
LRR_14 PF23598 649 - 732 4.3e-09 Leucine-rich repeat region
LRR_14 PF23598 734 - 782 3.9e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 898
Acc36I ACCTGC 2 cut(s) 249, 1836
AccB1I GGYRCC 1 cut(s) 716
AccB7I CCANNNNNTGG 1 cut(s) 917
AccII CGCG 1 cut(s) 951
AciI CCGC 4 cut(s) 15, 29, 949, 1092
AclI AACGTT 2 cut(s) 384, 1042
AclWI GGATC 2 cut(s) 838, 2310
AcoI YGGCCR 1 cut(s) 1903
AcsI RAATTY 4 cut(s) 632, 836, 1824, 2095
AcuI CTGAAG 7 cut(s) 296, 797, 1139, 1742, 2048, 2094, 2238
AfaI GTAC 3 cut(s) 683, 1734, 2125
AfiI CCNNNNNNNGG 4 cut(s) 101, 102, 103, 917
AflII CTTAAG 1 cut(s) 2204
AflIII ACRYGT 1 cut(s) 2327
AhdI GACNNNNNGTC 1 cut(s) 544
AhlI ACTAGT 1 cut(s) 1780
AjnI CCWGG 3 cut(s) 568, 720, 810
AjuI GAANNNNNNNTTGG 4 cut(s) 350, 382, 904, 936
Alw21I GWGCWC 5 cut(s) 234, 248, 402, 1004, 2270
Alw26I GTCTC 4 cut(s) 1501, 1524, 2254, 2333
Alw44I GTGCAC 1 cut(s) 398
AlwI GGATC 2 cut(s) 838, 2310
AlwNI CAGNNNCTG 4 cut(s) 82, 139, 161, 319
Ama87I CYCGRG 2 cut(s) 101, 2269
AoxI GGCC 1 cut(s) 1903
ApaLI GTGCAC 1 cut(s) 398
ApeKI GCWGC 8 cut(s) 133, 282, 526, 763, 766, 980, 1096, 2104
ApoI RAATTY 4 cut(s) 632, 836, 1824, 2095
Asp700I GAANNNNTTC 2 cut(s) 840, 1020
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 5 cut(s) 73, 179, 724, 808, 1754
AsuC2I CCSGG 1 cut(s) 2145
AsuHPI GGTGA 7 cut(s) 16, 43, 565, 901, 965, 1004, 2035
AsuII TTCGAA 1 cut(s) 1790
AvaI CYCGRG 2 cut(s) 101, 2269
AvaII GGWCC 5 cut(s) 73, 179, 724, 808, 1754
AxyI CCTNAGG 1 cut(s) 988
BaeGI GKGCMC 2 cut(s) 402, 721
BaeI ACNNNNGTAYC 4 cut(s) 71, 104, 1547, 1580
BalI TGGCCA 1 cut(s) 1905
BanI GGYRCC 1 cut(s) 716
BanII GRGCYC 2 cut(s) 234, 1004
BbsI GAAGAC 5 cut(s) 640, 1730, 2082, 2226, 2412
Bbv12I GWGCWC 5 cut(s) 234, 248, 402, 1004, 2270
BbvCI CCTCAGC 1 cut(s) 2108
BbvI GCAGC 8 cut(s) 145, 294, 538, 750, 778, 992, 1108, 2091
BccI CCATC 5 cut(s) 145, 170, 544, 1400, 1406
BciT130I CCWGG 3 cut(s) 570, 722, 812
BciVI GTATCC 1 cut(s) 1860
BclI TGATCA 1 cut(s) 141
BcnI CCSGG 1 cut(s) 2145
BcoDI GTCTC 4 cut(s) 1501, 1524, 2254, 2333
BcuI ACTAGT 1 cut(s) 1780
BfaI CTAG 7 cut(s) 194, 1137, 1730, 1781, 1817, 2090, 2431
BfmI CTRYAG 1 cut(s) 764
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 2204
BfuAI ACCTGC 2 cut(s) 249, 1836
BfuI GTATCC 1 cut(s) 1860
BglII AGATCT 1 cut(s) 1018
BisI GCNGC 8 cut(s) 134, 283, 527, 764, 767, 981, 1097, 2105
BlsI GCNGC 8 cut(s) 135, 284, 528, 765, 768, 982, 1098, 2106
BmcAI AGTACT 1 cut(s) 1734
Bme1390I CCNGG 4 cut(s) 570, 722, 812, 2145
Bme18I GGWCC 5 cut(s) 73, 179, 724, 808, 1754
BmeRI GACNNNNNGTC 1 cut(s) 544
BmeT110I CYCGRG 2 cut(s) 101, 2269
BmgT120I GGNCC 5 cut(s) 73, 179, 724, 808, 1754
BmiI GGNNCC 7 cut(s) 718, 726, 1488, 1924, 2159, 2358, 2426
BmrFI CCNGG 4 cut(s) 570, 722, 812, 2145
BmsI GCATC 9 cut(s) 354, 414, 513, 604, 946, 992, 1189, 1198, 2022
BpiI GAAGAC 5 cut(s) 640, 1730, 2082, 2226, 2412
BplI GAGNNNNNCTC 2 cut(s) 216, 248
BpmI CTGGAG 1 cut(s) 552
Bpu10I CCTNAGC 3 cut(s) 1454, 2108, 2114
Bpu14I TTCGAA 1 cut(s) 1790
BpuEI CTTGAG 3 cut(s) 319, 866, 2147
BpuMI CCSGG 1 cut(s) 2145
BsaJI CCNNGG 4 cut(s) 720, 911, 1246, 1906
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 102, 103, 917
Bse1I ACTGG 2 cut(s) 177, 597
Bse21I CCTNAGG 1 cut(s) 988
Bse3DI GCAATG 3 cut(s) 649, 966, 1987
BseBI CCWGG 3 cut(s) 570, 722, 812
BseDI CCNNGG 4 cut(s) 720, 911, 1246, 1906
BseGI GGATG 7 cut(s) 181, 358, 581, 820, 983, 1213, 1411
BseLI CCNNNNNNNGG 4 cut(s) 101, 102, 103, 917
BseMI GCAATG 3 cut(s) 649, 966, 1987
BseMII CTCAG 6 cut(s) 153, 1686, 1752, 2050, 2099, 2389
BseNI ACTGG 2 cut(s) 177, 597
BseRI GAGGAG 2 cut(s) 200, 2351
BseSI GKGCMC 2 cut(s) 402, 721
BseXI GCAGC 8 cut(s) 145, 294, 538, 750, 778, 992, 1108, 2091
BsgI GTGCAG 2 cut(s) 359, 2257
Bsh1236I CGCG 1 cut(s) 951
BshFI GGCC 1 cut(s) 1905
BshNI GGYRCC 1 cut(s) 716
BsiHKAI GWGCWC 5 cut(s) 234, 248, 402, 1004, 2270
BsiHKCI CYCGRG 2 cut(s) 101, 2269
BsiSI CCGG 1 cut(s) 2145
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 4 cut(s) 101, 102, 103, 917
BsmAI GTCTC 4 cut(s) 1501, 1524, 2254, 2333
BsmBI CGTCTC 1 cut(s) 1524
BsmFI GGGAC 1 cut(s) 710
BsmI GAATGC 1 cut(s) 1238
BsnI GGCC 1 cut(s) 1905
BsoBI CYCGRG 2 cut(s) 101, 2269
Bsp119I TTCGAA 1 cut(s) 1790
Bsp1286I GDGCHC 6 cut(s) 234, 248, 402, 721, 1004, 2270
Bsp1407I TGTACA 1 cut(s) 2123
Bsp143I GATC 7 cut(s) 141, 830, 1018, 1082, 1264, 2005, 2302
BspACI CCGC 4 cut(s) 15, 29, 949, 1092
BspANI GGCC 1 cut(s) 1905
BspCNI CTCAG 6 cut(s) 154, 1687, 1753, 2049, 2100, 2388
BspFNI CGCG 1 cut(s) 951
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 7 cut(s) 718, 726, 1488, 1924, 2159, 2358, 2426
BspMAI CTGCAG 1 cut(s) 768
BspMI ACCTGC 2 cut(s) 249, 1836
BspPI GGATC 2 cut(s) 838, 2310
BspT104I TTCGAA 1 cut(s) 1790
BspT107I GGYRCC 1 cut(s) 716
BspTI CTTAAG 1 cut(s) 2204
BsrDI GCAATG 3 cut(s) 649, 966, 1987
BsrGI TGTACA 1 cut(s) 2123
BsrI ACTGG 2 cut(s) 177, 597
BssECI CCNNGG 4 cut(s) 720, 911, 1246, 1906
BssMI GATC 7 cut(s) 141, 830, 1018, 1082, 1264, 2005, 2302
BssT1I CCWWGG 3 cut(s) 911, 1246, 1906
Bst2UI CCWGG 3 cut(s) 570, 722, 812
Bst4CI ACNGT 7 cut(s) 490, 661, 1285, 1860, 1922, 2265, 2370
Bst6I CTCTTC 1 cut(s) 1602
BstAFI CTTAAG 1 cut(s) 2204
BstAPI GCANNNNNTGC 2 cut(s) 1450, 1633
BstAUI TGTACA 1 cut(s) 2123
BstBI TTCGAA 1 cut(s) 1790
BstC8I GCNNGC 5 cut(s) 256, 761, 1094, 1582, 2001
BstF5I GGATG 7 cut(s) 181, 358, 581, 820, 983, 1213, 1411
BstFNI CGCG 1 cut(s) 951
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 7 cut(s) 144, 833, 1021, 1085, 1267, 2008, 2305
BstMAI GTCTC 4 cut(s) 1501, 1524, 2254, 2333
BstMBI GATC 7 cut(s) 141, 830, 1018, 1082, 1264, 2005, 2302
BstMWI GCNNNNNNNGC 5 cut(s) 26, 1208, 1450, 1633, 2110
BstNI CCWGG 3 cut(s) 570, 722, 812
BstNSI RCATGY 1 cut(s) 2331
BstSCI CCNGG 4 cut(s) 568, 720, 810, 2143
BstSFI CTRYAG 1 cut(s) 764
BstSLI GKGCMC 2 cut(s) 402, 721
BstUI CGCG 1 cut(s) 951
BstV1I GCAGC 8 cut(s) 145, 294, 538, 750, 778, 992, 1108, 2091
BstV2I GAAGAC 5 cut(s) 640, 1730, 2082, 2226, 2412
BstX2I RGATCY 2 cut(s) 1018, 2302
BstXI CCANNNNNNTGG 1 cut(s) 1913
BstYI RGATCY 2 cut(s) 1018, 2302
Bsu36I CCTNAGG 1 cut(s) 988
BsuI GTATCC 1 cut(s) 1860
BsuRI GGCC 1 cut(s) 1905
BtgZI GCGATG 1 cut(s) 1425
BtsCI GGATG 7 cut(s) 181, 358, 581, 820, 983, 1213, 1411
BtsIMutI CAGTG 6 cut(s) 137, 590, 1281, 1422, 2044, 2270
BveI ACCTGC 2 cut(s) 249, 1836
Cac8I GCNNGC 5 cut(s) 256, 761, 1094, 1582, 2001
CaiI CAGNNNCTG 4 cut(s) 82, 139, 161, 319
CciI TCATGA 1 cut(s) 610
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 5 cut(s) 73, 179, 724, 808, 1754
Csp6I GTAC 3 cut(s) 682, 1733, 2124
CviAII CATG 7 cut(s) 611, 977, 1166, 1445, 1891, 2257, 2328
CviQI GTAC 3 cut(s) 682, 1733, 2124
DpnI GATC 7 cut(s) 143, 832, 1020, 1084, 1266, 2007, 2304
DpnII GATC 7 cut(s) 141, 830, 1018, 1082, 1264, 2005, 2302
DraI TTTAAA 1 cut(s) 535
DrdI GACNNNNNNGTC 1 cut(s) 898
DriI GACNNNNNGTC 1 cut(s) 544
DseDI GACNNNNNNGTC 1 cut(s) 898
EaeI YGGCCR 1 cut(s) 1903
Eam1104I CTCTTC 1 cut(s) 1602
Eam1105I GACNNNNNGTC 1 cut(s) 544
EarI CTCTTC 1 cut(s) 1602
Ecl136II GAGCTC 2 cut(s) 232, 1002
Eco130I CCWWGG 3 cut(s) 911, 1246, 1906
Eco24I GRGCYC 2 cut(s) 234, 1004
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 5 cut(s) 73, 179, 724, 808, 1754
Eco53kI GAGCTC 2 cut(s) 232, 1002
Eco57I CTGAAG 7 cut(s) 296, 797, 1139, 1742, 2048, 2094, 2238
Eco81I CCTNAGG 1 cut(s) 988
Eco88I CYCGRG 2 cut(s) 101, 2269
EcoICRI GAGCTC 2 cut(s) 232, 1002
EcoO109I RGGNCCY 1 cut(s) 724
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 3 cut(s) 568, 720, 810
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 3 cut(s) 911, 1246, 1906
EcoT38I GRGCYC 2 cut(s) 234, 1004
ErhI CCWWGG 3 cut(s) 911, 1246, 1906
Esp3I CGTCTC 1 cut(s) 1524
FaeI CATG 7 cut(s) 614, 980, 1169, 1448, 1894, 2260, 2331
FalI AAGNNNNNCTT 2 cut(s) 889, 921
FaqI GGGAC 1 cut(s) 710
FatI CATG 7 cut(s) 610, 976, 1165, 1444, 1890, 2256, 2327
FauI CCCGC 1 cut(s) 1085
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 8 cut(s) 134, 283, 527, 764, 767, 981, 1097, 2105
FokI GGATG 7 cut(s) 188, 365, 588, 827, 970, 1220, 1418
FriOI GRGCYC 2 cut(s) 234, 1004
Fsp4HI GCNGC 8 cut(s) 134, 283, 527, 764, 767, 981, 1097, 2105
FspBI CTAG 7 cut(s) 194, 1137, 1730, 1781, 1817, 2090, 2431
GlaI GCGC 1 cut(s) 45
GluI GCNGC 8 cut(s) 134, 283, 527, 764, 767, 981, 1097, 2105
GsuI CTGGAG 1 cut(s) 552
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 1 cut(s) 1905
HapII CCGG 1 cut(s) 2145
HhaI GCGC 1 cut(s) 46
Hin1II CATG 7 cut(s) 614, 980, 1169, 1448, 1894, 2260, 2331
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 7 cut(s) 53, 125, 158, 199, 1272, 1726, 1809
HpaII CCGG 1 cut(s) 2145
HphI GGTGA 7 cut(s) 16, 43, 565, 901, 965, 1004, 2035
Hpy166II GTNNAC 4 cut(s) 117, 295, 400, 1345
Hpy8I GTNNAC 4 cut(s) 117, 295, 400, 1345
HpyAV CCTTC 8 cut(s) 64, 160, 215, 355, 404, 446, 1845, 2298
HpyCH4III ACNGT 7 cut(s) 490, 661, 1285, 1860, 1922, 2265, 2370
HpyCH4IV ACGT 2 cut(s) 384, 1042
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 1208, 1450, 1633, 2110
HpySE526I ACGT 2 cut(s) 384, 1042
Hsp92II CATG 7 cut(s) 614, 980, 1169, 1448, 1894, 2260, 2331
HspAI GCGC 1 cut(s) 44
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 7 cut(s) 141, 830, 1018, 1082, 1264, 2005, 2302
LmnI GCTCC 6 cut(s) 17, 229, 571, 999, 2356, 2430
Lsp1109I GCAGC 8 cut(s) 145, 294, 538, 750, 778, 992, 1108, 2091
LweI GCATC 9 cut(s) 354, 414, 513, 604, 946, 992, 1189, 1198, 2022
MaeI CTAG 7 cut(s) 194, 1137, 1730, 1781, 1817, 2090, 2431
MaeII ACGT 2 cut(s) 384, 1042
MalI GATC 7 cut(s) 143, 832, 1020, 1084, 1266, 2007, 2304
MboI GATC 7 cut(s) 141, 830, 1018, 1082, 1264, 2005, 2302
MflI RGATCY 2 cut(s) 1018, 2302
MhlI GDGCHC 6 cut(s) 234, 248, 402, 721, 1004, 2270
MlsI TGGCCA 1 cut(s) 1905
MluNI TGGCCA 1 cut(s) 1905
MlyI GAGTC 3 cut(s) 119, 167, 1720
MmeI TCCRAC 4 cut(s) 472, 503, 1057, 1495
Mox20I TGGCCA 1 cut(s) 1905
MroXI GAANNNNTTC 2 cut(s) 840, 1020
MscI TGGCCA 1 cut(s) 1905
MseI TTAA 7 cut(s) 60, 534, 863, 1068, 2171, 2205, 2315
MslI CAYNNNNRTG 2 cut(s) 1295, 1621
Msp20I TGGCCA 1 cut(s) 1905
MspCI CTTAAG 1 cut(s) 2204
MspI CCGG 1 cut(s) 2145
MspR9I CCNGG 4 cut(s) 570, 722, 812, 2145
Mva1269I GAATGC 1 cut(s) 1238
MvaI CCWGG 3 cut(s) 570, 722, 812
MvnI CGCG 1 cut(s) 951
MwoI GCNNNNNNNGC 5 cut(s) 26, 1208, 1450, 1633, 2110
NciI CCSGG 1 cut(s) 2145
NdeII GATC 7 cut(s) 141, 830, 1018, 1082, 1264, 2005, 2302
NlaIII CATG 7 cut(s) 614, 980, 1169, 1448, 1894, 2260, 2331
NlaIV GGNNCC 7 cut(s) 718, 726, 1488, 1924, 2159, 2358, 2426
NmuCI GTSAC 7 cut(s) 31, 306, 889, 1387, 2012, 2041, 2323
NspI RCATGY 1 cut(s) 2331
NspV TTCGAA 1 cut(s) 1790
PaeR7I CTCGAG 1 cut(s) 2269
PagI TCATGA 1 cut(s) 610
PciI ACATGT 1 cut(s) 2327
PctI GAATGC 1 cut(s) 1238
PdmI GAANNNNTTC 2 cut(s) 840, 1020
PfeI GAWTC 4 cut(s) 53, 199, 1272, 1809
PflMI CCANNNNNTGG 1 cut(s) 917
PfoI TCCNGGA 2 cut(s) 568, 810
PkrI GCNGC 8 cut(s) 135, 284, 528, 765, 768, 982, 1098, 2106
PleI GAGTC 3 cut(s) 119, 166, 1720
PpsI GAGTC 3 cut(s) 119, 166, 1720
PpuMI RGGWCCY 1 cut(s) 724
PscI ACATGT 1 cut(s) 2327
Psp124BI GAGCTC 2 cut(s) 234, 1004
Psp1406I AACGTT 2 cut(s) 384, 1042
Psp5II RGGWCCY 1 cut(s) 724
Psp6I CCWGG 3 cut(s) 568, 720, 810
PspGI CCWGG 3 cut(s) 568, 720, 810
PspN4I GGNNCC 7 cut(s) 718, 726, 1488, 1924, 2159, 2358, 2426
PspPI GGNCC 5 cut(s) 73, 179, 724, 808, 1754
PspPPI RGGWCCY 1 cut(s) 724
PstI CTGCAG 1 cut(s) 768
PstNI CAGNNNCTG 4 cut(s) 82, 139, 161, 319
PsuI RGATCY 2 cut(s) 1018, 2302
RsaI GTAC 3 cut(s) 683, 1734, 2125
RsaNI GTAC 3 cut(s) 682, 1733, 2124
RseI CAYNNNNRTG 2 cut(s) 1295, 1621
SacI GAGCTC 2 cut(s) 234, 1004
SaqAI TTAA 7 cut(s) 60, 534, 863, 1068, 2171, 2205, 2315
SatI GCNGC 8 cut(s) 134, 283, 527, 764, 767, 981, 1097, 2105
Sau3AI GATC 7 cut(s) 141, 830, 1018, 1082, 1264, 2005, 2302
Sau96I GGNCC 5 cut(s) 73, 179, 724, 808, 1754
ScaI AGTACT 1 cut(s) 1734
SchI GAGTC 3 cut(s) 119, 167, 1720
ScrFI CCNGG 4 cut(s) 570, 722, 812, 2145
SduI GDGCHC 6 cut(s) 234, 248, 402, 721, 1004, 2270
SfaNI GCATC 9 cut(s) 354, 414, 513, 604, 946, 992, 1189, 1198, 2022
SfcI CTRYAG 1 cut(s) 764
Sfr274I CTCGAG 1 cut(s) 2269
SfuI TTCGAA 1 cut(s) 1790
SinI GGWCC 5 cut(s) 73, 179, 724, 808, 1754
SlaI CTCGAG 1 cut(s) 2269
SmiMI CAYNNNNRTG 2 cut(s) 1295, 1621
SmlI CTYRAG 5 cut(s) 298, 845, 2162, 2204, 2269
SmoI CTYRAG 5 cut(s) 298, 845, 2162, 2204, 2269
SpeI ACTAGT 1 cut(s) 1780
SsiI CCGC 4 cut(s) 15, 29, 949, 1092
SspI AATATT 1 cut(s) 1969
SspMI CTAG 7 cut(s) 194, 1137, 1730, 1781, 1817, 2090, 2431
SstI GAGCTC 2 cut(s) 234, 1004
StyD4I CCNGG 4 cut(s) 568, 720, 810, 2143
StyI CCWWGG 3 cut(s) 911, 1246, 1906
TaaI ACNGT 7 cut(s) 490, 661, 1285, 1860, 1922, 2265, 2370
TaiI ACGT 2 cut(s) 387, 1045
TaqI TCGA 8 cut(s) 548, 709, 855, 1366, 1790, 2150, 2189, 2270
TaqII GACCGA 1 cut(s) 1742
TatI WGTACW 2 cut(s) 1732, 2123
TfiI GAWTC 4 cut(s) 53, 199, 1272, 1809
Tru1I TTAA 7 cut(s) 60, 534, 863, 1068, 2171, 2205, 2315
Tru9I TTAA 7 cut(s) 60, 534, 863, 1068, 2171, 2205, 2315
TscAI CASTG 6 cut(s) 144, 597, 1288, 1429, 2044, 2270
TseFI GTSAC 7 cut(s) 31, 306, 889, 1387, 2012, 2041, 2323
TseI GCWGC 8 cut(s) 133, 282, 526, 763, 766, 980, 1096, 2104
Tsp45I GTSAC 7 cut(s) 31, 306, 889, 1387, 2012, 2041, 2323
TspDTI ATGAA 8 cut(s) 829, 873, 1368, 1727, 1818, 1879, 1979, 2280
TspGWI ACGGA 2 cut(s) 364, 555
TspRI CASTG 6 cut(s) 144, 597, 1288, 1429, 2044, 2270
Van91I CCANNNNNTGG 1 cut(s) 917
Vha464I CTTAAG 1 cut(s) 2204
VneI GTGCAC 1 cut(s) 398
VpaK11BI GGWCC 5 cut(s) 73, 179, 724, 808, 1754
XapI RAATTY 4 cut(s) 632, 836, 1824, 2095
XbaI TCTAGA 1 cut(s) 1136
XceI RCATGY 1 cut(s) 2331
XcmI CCANNNNNNNNNTGG 1 cut(s) 1107
XhoI CTCGAG 1 cut(s) 2269
XmnI GAANNNNTTC 2 cut(s) 840, 1020
XspI CTAG 7 cut(s) 194, 1137, 1730, 1781, 1817, 2090, 2431
ZrmI AGTACT 1 cut(s) 1734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.