MD02G1165400.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000039
Physical Location & Seq
Forward (+)
14150715 .. 14155598
4884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1165400.v1.1.491

Sequence Viewer

Length: 2397 bp
ATGGAGCTGATCGCCAAGAATGTAAGGATTAAGCCCCTCCTCAAAGACATCAAATCCAGGTTAGACTCTTTGGAACCATTGTTGAAATATATAGAAAAAGATATAGAAAAATCCAAGAAATTGGATCTTCCGGTTGTGGAACTGGAAAACTTAGAAGTGCATTTGGAAGAGGGCATAGAGCTCGTTCAAAAGTGCATAAAGATTCGACGGTGGACCACATACAAACGGTACAAATACTCCAACAAGCTGACTGGATGGGATAAATCTCTTCAACGACTTTTGGAAAAGCTAGACGTTCAGGGAATAAGGGATATGAAGGACAGCCCGGTTTTGGTGAAGAATATAGAGAAGGCGGTCAGCAAAATTCAATCGAATCTGGGGATACCAAAACAACCCGACAGTGAAACTTGGTGTGCAGCACCTGAACTTCCACCGCTTGTGGTTGGGTTGGATTTTCCTCTAAAGGAATTGAAGAGGAAGCTTCTAAAGGACGAAAAGGTATCAATGGTTGTGCTGACTGCTCCCGGAGGATGTGGGAAAACCACTTTGGCTACAAAGTTTTGTCAAGACAAGGATGTCAAAGATACATTCAAGGATAATATCTTCTTTGTCACGGTTTCAAAAAAGCCTAAATTGGAAAACATTGTTCAAGATCTATATCAACGAAAGGGTTTCCAGGTACCTACTTTCCAAAACGAAGTTAGTACAGTGACGTGGCTGCAACATTTTCTTAAGAAAGAAGGGCAGAATCCTATACTGTTTGTCCTGGATGATGTTTGGTCTGGATCAGAATCCCTTGTGCAGAAGTTTGATCAATTCAAAATGGAAAATTACAAGTTTTTGGTGACATCTAGGTCTGAATTTCGGGGATATGGTTCCCCTTATTATTTGCGATCATTGGATCGTGACAATGCGATGAAACTTTTTCATCATTCAGCATCCCTTGGAGATAAGAGCTCTCATATTCCAAAAGTTCTTGCAGAAAAGATAGTCGAACGTTGCAAGGGATTTCCGCTTGCTATTACAGTGGTGGGGAGATCGCTTTGCGGGGAGCCTATAGAGATATGGCAAAAAAGAGAACTTGAATGGTCTAAAGGTTTTTCTATTCTTGATTCAGAGACTGAATTGCTGCTCTGCCTTGAACGAAGCTTGGATGCCATAGATAAAGAAATCATCAAGGAATGTTTCTTAGACTTGGGTTCATTTCCAGAAGACCAAAGAATCCCTGTTGCTGCCCTCATTGATATCTGGGCAGAGTTATATGATGTAGATACAGATATAGAGTGCATTGCAAACCTCTATGAGCTAACCAAACGTAGTCTAGCTAATCTTGTTGTTACAAGGAAGGACAATATGGTGGGGGACGGGTACTACAGTGAGCACTTTGTTACCCATCATGATGTTGTTAGGGAGCTGGCTATCTACAACACAAAAAAAGGACCAGTAGAACATAGAAAAAAACTGATTATTGACATATGTGGAGACAATCTTCCAAAATGGTGGAGAGAACAAAAGCATCAGCCAATGAAGGCCCGCTTAATATCCATAACAACTGATGAAGGGTTTTCAACAAAGTGGCACGACATGCAAATGCCAGAAGCTAAGGTTTTAGTATTGAATTTCCAGACAAAGAATTATGCCTTACCCGAATCTTTGGTGAACATGGATGAATTGAAGGCTGTGATAGTCACAAATTATGGTTTCTTACCTGCTGAATTGAGTAACTTTCAACTTTTGAGTTCGTTACCAAATCTTAAGAGAATCAGATTAGAACGCATCTCAATTCCTTCTATAACCAACAATCCCAAACAATTGGCGGGTCTGAAGAAGATATCCTTGTTCATGTGTAACATTGGTCAAGCTTTCAACAACGGTTCCATCTCAATCTCATCAGCATTCCCAAATCTAGAGGAAATGAACATCGACTATTGCAGTGATTTAATTGAATTGCCCGTCGAGGTTTGTGATCTTAATAAACTAAAGAAGCTCGGTGTCACTAGCTGTCATAAACTATCTGCCTTGCCTGAAAGGATTGGAAAGTTGGAAAATTTAGAAATGTTAAGGCTAAGATCCTGCACAGAACTGTCAAAGCTTCCACCCTCAATGAAGAACCTGGGAAAGGTAAACTTTCTTGACATATCTGATTGCTTCAACATCAAGGTGTTGCCCGAAGACATTGGTGAAATGAGTAGTTTAAGAAAGATCAACATGAGACAATGTTCAAGGTTGCAAGAGCTGCCTCCATCAGTCATGGATCTTGAGCAGTTAGAGGAAGTGGTCTGTGATGAAGAGACAAAATATTTGTGGGAGTCTTTCTCGTCGTGTCTCAACAACCTGAAGATTATTGTGACCAAAGAGAATATCAACCTAAATTGGCTCCACAAGACTCAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

799

Amino Acids

91.7

Weight (kDa)

7.44

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 113 9.7e-14 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 154 - 312 5.8e-16 NB-ARC domain
LRR_14 PF23598 630 - 782 1.6e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 575, 853
Acc36I ACCTGC 1 cut(s) 1717
Acc65I GGTACC 1 cut(s) 679
AccB1I GGYRCC 1 cut(s) 679
AciI CCGC 6 cut(s) 353, 434, 1013, 1047, 1534, 1817
AclI AACGTT 1 cut(s) 997
AclWI GGATC 5 cut(s) 132, 793, 909, 2064, 2262
AcsI RAATTY 4 cut(s) 363, 860, 1618, 2047
AcuI CTGAAG 2 cut(s) 1844, 2357
AfaI GTAC 4 cut(s) 230, 681, 706, 1370
AfiI CCNNNNNNNGG 2 cut(s) 331, 2119
AflII CTTAAG 2 cut(s) 731, 1754
AjiI CACGTC 1 cut(s) 714
AjnI CCWGG 4 cut(s) 56, 675, 765, 2112
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
Alw21I GWGCWC 3 cut(s) 183, 959, 1383
Alw26I GTCTC 5 cut(s) 1112, 1476, 2206, 2285, 2330
AlwI GGATC 5 cut(s) 132, 793, 909, 2064, 2262
AlwNI CAGNNNCTG 2 cut(s) 422, 1121
AoxI GGCC 1 cut(s) 1530
ApeKI GCWGC 5 cut(s) 416, 718, 1129, 1232, 2236
ApoI RAATTY 4 cut(s) 363, 860, 1618, 2047
ArsI GACNNNNNNTTYG 2 cut(s) 2284, 2316
Asp718I GGTACC 1 cut(s) 679
AspS9I GGNCC 3 cut(s) 213, 1439, 1531
AsuC2I CCSGG 2 cut(s) 326, 525
AsuHPI GGTGA 4 cut(s) 346, 856, 1669, 2192
AvaII GGWCC 2 cut(s) 213, 1439
BaeI ACNNNNGTAYC 2 cut(s) 1360, 1393
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 2 cut(s) 183, 959
BbsI GAAGAC 2 cut(s) 1218, 2178
Bbv12I GWGCWC 3 cut(s) 183, 959, 1383
BbvI GCAGC 5 cut(s) 428, 705, 1116, 1219, 2223
BccI CCATC 4 cut(s) 249, 1401, 1886, 2251
BcgI CGANNNNNNTGC 2 cut(s) 163, 197
BciT130I CCWGG 4 cut(s) 58, 677, 767, 2114
BciVI GTATCC 1 cut(s) 375
BclI TGATCA 1 cut(s) 811
BcnI CCSGG 2 cut(s) 326, 525
BcoDI GTCTC 5 cut(s) 1112, 1476, 2206, 2285, 2330
BfaI CTAG 5 cut(s) 290, 852, 1322, 1907, 1998
BfmI CTRYAG 2 cut(s) 1056, 1372
BfrI CTTAAG 2 cut(s) 731, 1754
BfuAI ACCTGC 1 cut(s) 1717
BfuI GTATCC 1 cut(s) 375
BglII AGATCT 1 cut(s) 652
BisI GCNGC 5 cut(s) 417, 719, 1130, 1233, 2237
BlsI GCNGC 5 cut(s) 418, 720, 1131, 1234, 2238
Bme1390I CCNGG 6 cut(s) 58, 326, 525, 677, 767, 2114
Bme18I GGWCC 2 cut(s) 213, 1439
BmgBI CACGTC 1 cut(s) 714
BmgT120I GGNCC 3 cut(s) 213, 1439, 1531
BmiI GGNNCC 6 cut(s) 75, 681, 877, 1053, 1876, 2378
BmrFI CCNGG 6 cut(s) 58, 326, 525, 677, 767, 2114
BmsI GCATC 4 cut(s) 947, 1144, 1525, 1785
BpiI GAAGAC 2 cut(s) 1218, 2178
BplI GAGNNNNNCTC 2 cut(s) 2300, 2332
Bpu10I CCTNAGC 1 cut(s) 1602
BpuEI CTTGAG 1 cut(s) 2279
BpuMI CCSGG 2 cut(s) 326, 525
BsaBI GATNNNNATC 2 cut(s) 657, 790
BsaJI CCNNGG 2 cut(s) 943, 2113
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 2119
Bse1I ACTGG 3 cut(s) 147, 256, 1442
Bse3DI GCAATG 1 cut(s) 1287
Bse8I GATNNNNATC 2 cut(s) 657, 790
BseBI CCWGG 4 cut(s) 58, 677, 767, 2114
BseDI CCNNGG 2 cut(s) 943, 2113
BseGI GGATG 7 cut(s) 260, 536, 580, 775, 938, 1159, 1672
BseJI GATNNNNATC 2 cut(s) 657, 790
BseLI CCNNNNNNNGG 2 cut(s) 331, 2119
BseMI GCAATG 1 cut(s) 1287
BseNI ACTGG 3 cut(s) 147, 256, 1442
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 5 cut(s) 428, 705, 1116, 1219, 2223
BsgI GTGCAG 3 cut(s) 435, 821, 2059
BshFI GGCC 1 cut(s) 1532
BshNI GGYRCC 1 cut(s) 679
BsiHKAI GWGCWC 3 cut(s) 183, 959, 1383
BsiSI CCGG 3 cut(s) 131, 326, 525
BslFI GGGAC 1 cut(s) 1376
BslI CCNNNNNNNGG 2 cut(s) 331, 2119
BsmAI GTCTC 5 cut(s) 1112, 1476, 2206, 2285, 2330
BsmFI GGGAC 1 cut(s) 1376
BsmI GAATGC 1 cut(s) 1895
BsnI GGCC 1 cut(s) 1532
Bsp1286I GDGCHC 3 cut(s) 183, 959, 1383
BspACI CCGC 6 cut(s) 353, 434, 1013, 1047, 1534, 1817
BspANI GGCC 1 cut(s) 1532
BspHI TCATGA 1 cut(s) 1396
BspLI GGNNCC 6 cut(s) 75, 681, 877, 1053, 1876, 2378
BspMI ACCTGC 1 cut(s) 1717
BspPI GGATC 5 cut(s) 132, 793, 909, 2064, 2262
BspT107I GGYRCC 1 cut(s) 679
BspTI CTTAAG 2 cut(s) 731, 1754
BsrDI GCAATG 1 cut(s) 1287
BsrI ACTGG 3 cut(s) 147, 256, 1442
BssECI CCNNGG 2 cut(s) 943, 2113
BssT1I CCWWGG 1 cut(s) 943
Bst2UI CCWGG 4 cut(s) 58, 677, 767, 2114
Bst6I CTCTTC 4 cut(s) 162, 273, 467, 2283
BstAFI CTTAAG 2 cut(s) 731, 1754
BstAPI GCANNNNNTGC 2 cut(s) 1585, 2236
BstC8I GCNNGC 3 cut(s) 1017, 1416, 1534
BstDEI CTNAG 5 cut(s) 151, 1189, 1602, 2066, 2388
BstENI CCTNNNNNAGG 1 cut(s) 2117
BstF5I GGATG 7 cut(s) 260, 536, 580, 775, 938, 1159, 1672
BstMAI GTCTC 5 cut(s) 1112, 1476, 2206, 2285, 2330
BstMWI GCNNNNNNNGC 2 cut(s) 1585, 2236
BstNI CCWGG 4 cut(s) 58, 677, 767, 2114
BstNSI RCATGY 1 cut(s) 1588
BstSCI CCNGG 6 cut(s) 56, 324, 523, 675, 765, 2112
BstSFI CTRYAG 2 cut(s) 1056, 1372
BstV1I GCAGC 5 cut(s) 428, 705, 1116, 1219, 2223
BstV2I GAAGAC 2 cut(s) 1218, 2178
BstX2I RGATCY 4 cut(s) 124, 652, 2069, 2254
BstXI CCANNNNNNTGG 3 cut(s) 121, 1500, 1813
BstYI RGATCY 4 cut(s) 124, 652, 2069, 2254
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 1 cut(s) 1532
BtgZI GCGATG 1 cut(s) 929
BtrI CACGTC 1 cut(s) 714
BtsCI GGATG 7 cut(s) 260, 536, 580, 775, 938, 1159, 1672
BtsI GCAGTG 1 cut(s) 1939
BtsIMutI CAGTG 5 cut(s) 406, 714, 1032, 1381, 1939
BveI ACCTGC 1 cut(s) 1717
Cac8I GCNNGC 3 cut(s) 1017, 1416, 1534
CaiI CAGNNNCTG 2 cut(s) 422, 1121
CciI TCATGA 1 cut(s) 1396
Cfr13I GGNCC 3 cut(s) 213, 1439, 1531
Csp6I GTAC 4 cut(s) 229, 680, 705, 1369
CviAII CATG 6 cut(s) 1397, 1585, 1663, 1843, 2209, 2251
CviQI GTAC 4 cut(s) 229, 680, 705, 1369
DdeI CTNAG 5 cut(s) 151, 1189, 1602, 2066, 2388
DrdI GACNNNNNNGTC 2 cut(s) 575, 853
DseDI GACNNNNNNGTC 2 cut(s) 575, 853
Eam1104I CTCTTC 4 cut(s) 162, 273, 467, 2283
EarI CTCTTC 4 cut(s) 162, 273, 467, 2283
Ecl136II GAGCTC 2 cut(s) 181, 957
Eco130I CCWWGG 1 cut(s) 943
Eco24I GRGCYC 2 cut(s) 183, 959
Eco32I GATATC 2 cut(s) 1246, 1833
Eco47I GGWCC 2 cut(s) 213, 1439
Eco53kI GAGCTC 2 cut(s) 181, 957
Eco57I CTGAAG 2 cut(s) 1844, 2357
EcoICRI GAGCTC 2 cut(s) 181, 957
EcoNI CCTNNNNNAGG 1 cut(s) 2117
EcoRII CCWGG 4 cut(s) 56, 675, 765, 2112
EcoRV GATATC 2 cut(s) 1246, 1833
EcoT14I CCWWGG 1 cut(s) 943
EcoT38I GRGCYC 2 cut(s) 183, 959
ErhI CCWWGG 1 cut(s) 943
FaeI CATG 6 cut(s) 1400, 1588, 1666, 1846, 2212, 2254
FalI AAGNNNNNCTT 6 cut(s) 1520, 1552, 1820, 1852, 2111, 2143
FaqI GGGAC 1 cut(s) 1376
FatI CATG 6 cut(s) 1396, 1584, 1662, 1842, 2208, 2250
FauI CCCGC 3 cut(s) 1040, 1541, 1810
FauNDI CATATG 1 cut(s) 1475
FbaI TGATCA 1 cut(s) 811
Fnu4HI GCNGC 5 cut(s) 417, 719, 1130, 1233, 2237
FokI GGATG 7 cut(s) 267, 543, 587, 782, 925, 1166, 1679
FriOI GRGCYC 2 cut(s) 183, 959
Fsp4HI GCNGC 5 cut(s) 417, 719, 1130, 1233, 2237
FspBI CTAG 5 cut(s) 290, 852, 1322, 1907, 1998
GluI GCNGC 5 cut(s) 417, 719, 1130, 1233, 2237
HaeIII GGCC 1 cut(s) 1532
HapII CCGG 3 cut(s) 131, 326, 525
Hin1II CATG 6 cut(s) 1400, 1588, 1666, 1846, 2212, 2254
HindIII AAGCTT 4 cut(s) 479, 1147, 1860, 2090
HpaII CCGG 3 cut(s) 131, 326, 525
HphI GGTGA 4 cut(s) 346, 856, 1669, 2192
Hpy166II GTNNAC 3 cut(s) 213, 1660, 2125
Hpy188I TCNGA 7 cut(s) 790, 859, 1117, 1766, 1824, 2143, 2396
Hpy8I GTNNAC 3 cut(s) 213, 1660, 2125
Hpy99I CGWCG 3 cut(s) 210, 1958, 2323
HpyAV CCTTC 8 cut(s) 310, 343, 734, 1339, 1522, 1553, 1669, 1797
HpyCH4IV ACGT 4 cut(s) 294, 713, 997, 1315
HpyF10VI GCNNNNNNNGC 2 cut(s) 1585, 2236
HpyF3I CTNAG 5 cut(s) 151, 1189, 1602, 2066, 2388
HpySE526I ACGT 4 cut(s) 294, 713, 997, 1315
Hsp92II CATG 6 cut(s) 1400, 1588, 1666, 1846, 2212, 2254
KpnI GGTACC 1 cut(s) 683
Ksp22I TGATCA 1 cut(s) 811
LmnI GCTCC 5 cut(s) 4, 526, 1051, 1411, 2382
Lsp1109I GCAGC 5 cut(s) 428, 705, 1116, 1219, 2223
LweI GCATC 4 cut(s) 947, 1144, 1525, 1785
MaeI CTAG 5 cut(s) 290, 852, 1322, 1907, 1998
MaeII ACGT 4 cut(s) 294, 713, 997, 1315
MfeI CAATTG 1 cut(s) 1811
MflI RGATCY 4 cut(s) 124, 652, 2069, 2254
MhlI GDGCHC 3 cut(s) 183, 959, 1383
MlyI GAGTC 3 cut(s) 59, 2318, 2380
MmeI TCCRAC 3 cut(s) 264, 429, 2022
MseI TTAA 8 cut(s) 30, 732, 1538, 1755, 1940, 1971, 2060, 2195
MslI CAYNNNNRTG 3 cut(s) 1398, 1589, 2159
MspCI CTTAAG 2 cut(s) 731, 1754
MspI CCGG 3 cut(s) 131, 326, 525
MspR9I CCNGG 6 cut(s) 58, 326, 525, 677, 767, 2114
MunI CAATTG 1 cut(s) 1811
Mva1269I GAATGC 1 cut(s) 1895
MvaI CCWGG 4 cut(s) 58, 677, 767, 2114
MwoI GCNNNNNNNGC 2 cut(s) 1585, 2236
NciI CCSGG 2 cut(s) 326, 525
NdeI CATATG 1 cut(s) 1475
NlaIII CATG 6 cut(s) 1400, 1588, 1666, 1846, 2212, 2254
NlaIV GGNNCC 6 cut(s) 75, 681, 877, 1053, 1876, 2378
NmuCI GTSAC 7 cut(s) 610, 709, 844, 905, 1687, 1993, 2347
NspI RCATGY 1 cut(s) 1588
PagI TCATGA 1 cut(s) 1396
PctI GAATGC 1 cut(s) 1895
PfeI GAWTC 8 cut(s) 202, 373, 748, 791, 1112, 1221, 1649, 1761
PfoI TCCNGGA 2 cut(s) 523, 765
PkrI GCNGC 5 cut(s) 418, 720, 1131, 1234, 2238
PleI GAGTC 3 cut(s) 59, 2317, 2380
PpsI GAGTC 3 cut(s) 59, 2317, 2380
Psp124BI GAGCTC 2 cut(s) 183, 959
Psp1406I AACGTT 1 cut(s) 997
Psp6I CCWGG 4 cut(s) 56, 675, 765, 2112
PspGI CCWGG 4 cut(s) 56, 675, 765, 2112
PspN4I GGNNCC 6 cut(s) 75, 681, 877, 1053, 1876, 2378
PspPI GGNCC 3 cut(s) 213, 1439, 1531
PstNI CAGNNNCTG 2 cut(s) 422, 1121
PsuI RGATCY 4 cut(s) 124, 652, 2069, 2254
RsaI GTAC 4 cut(s) 230, 681, 706, 1370
RsaNI GTAC 4 cut(s) 229, 680, 705, 1369
RseI CAYNNNNRTG 3 cut(s) 1398, 1589, 2159
SacI GAGCTC 2 cut(s) 183, 959
SaqAI TTAA 8 cut(s) 30, 732, 1538, 1755, 1940, 1971, 2060, 2195
SatI GCNGC 5 cut(s) 417, 719, 1130, 1233, 2237
Sau96I GGNCC 3 cut(s) 213, 1439, 1531
SchI GAGTC 3 cut(s) 59, 2318, 2380
ScrFI CCNGG 6 cut(s) 58, 326, 525, 677, 767, 2114
SduI GDGCHC 3 cut(s) 183, 959, 1383
SfaNI GCATC 4 cut(s) 947, 1144, 1525, 1785
SfcI CTRYAG 2 cut(s) 1056, 1372
SinI GGWCC 2 cut(s) 213, 1439
SmiMI CAYNNNNRTG 3 cut(s) 1398, 1589, 2159
SmlI CTYRAG 3 cut(s) 731, 1754, 2258
SmoI CTYRAG 3 cut(s) 731, 1754, 2258
SsiI CCGC 6 cut(s) 353, 434, 1013, 1047, 1534, 1817
SspI AATATT 1 cut(s) 2300
SspMI CTAG 5 cut(s) 290, 852, 1322, 1907, 1998
SstI GAGCTC 2 cut(s) 183, 959
StyD4I CCNGG 6 cut(s) 56, 324, 523, 675, 765, 2112
StyI CCWWGG 1 cut(s) 943
TaiI ACGT 4 cut(s) 297, 716, 1000, 1318
TaqI TCGA 5 cut(s) 205, 371, 993, 1923, 1956
TatI WGTACW 1 cut(s) 704
TfiI GAWTC 8 cut(s) 202, 373, 748, 791, 1112, 1221, 1649, 1761
Tru1I TTAA 8 cut(s) 30, 732, 1538, 1755, 1940, 1971, 2060, 2195
Tru9I TTAA 8 cut(s) 30, 732, 1538, 1755, 1940, 1971, 2060, 2195
TscAI CASTG 5 cut(s) 406, 714, 1032, 1381, 1939
TseFI GTSAC 7 cut(s) 610, 709, 844, 905, 1687, 1993, 2347
TseI GCWGC 5 cut(s) 416, 718, 1129, 1232, 2236
Tsp45I GTSAC 7 cut(s) 610, 709, 844, 905, 1687, 1993, 2347
TspRI CASTG 5 cut(s) 406, 714, 1032, 1381, 1939
Vha464I CTTAAG 2 cut(s) 731, 1754
VpaK11BI GGWCC 2 cut(s) 213, 1439
XagI CCTNNNNNAGG 1 cut(s) 2117
XapI RAATTY 4 cut(s) 363, 860, 1618, 2047
XbaI TCTAGA 1 cut(s) 1906
XceI RCATGY 1 cut(s) 1588
XspI CTAG 5 cut(s) 290, 852, 1322, 1907, 1998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.