FvH4_6g50130

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000039
Physical Location & Seq
Reverse (-)
37363663 .. 37366946
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50130.t1

Sequence Viewer

Length: 2721 bp
ATGGCGGTTGTCGGGGATTTTCTTGTAGGGAAAGCGCTCGAGTTACTGTATGGTGGTGTTGAAACGTTGATTGCCAATAATCTACTCTTTGAATCACTCTCCACCGACATCAAGGCCAAGCTAGACGCTTTGAGACCGATGATCCAGAAGATGGTACAACGTAATCACCAACCGGATTGTCCTGAGGATCCAGTTTTAAGAGATTTACGAGTATTAATGGAGGAAGGAGCAGTGGTCGTCTGTAAGTGCTTCTCTAAGATTCGTATTTGGAACTTCTATAAGAAGTACAAGTACACCAACAAACTTCTCAAGTTGAATGAAGCTCTTGAGAAGCAGTTAAGTTTACTGAATGTGCGTGTAGCAGTTGTCACAGACGAGAACAATGGTATACTACATGAGCTCTTAGATATTGCGAATCAAAACAAATCTGCAGCAATTGAAGCAGGTTGTGCACTACCTGAACTACCGTCACTTATAGTTGGATTGGATATTCCACTGAAGGAATTGAAGATGGAGTTTTTTAAGGATGAAGTATCACTGCTTGTATTGACTGCTCCAGGAGGATGTGGGAAAACTACTCTGGCAACAAAGTTCTGTCAAGATCAAGACGTCAAAGATAAGTTCAAGGACAACATCTTCTTCGTCACTGTTTCTAAAAAGCCTAAGTTGGAGCTTATCATGACAGAGCTATACAAACAAAAGGATTATAAGGTACCTACTTTCCAGAATGGAGTATTTCCAGTGAAGTGGCTACAACAAATTGTGAAGGATGAAGGTGATAATCCTTTGTTATTGGTCTTGGATGATGTTTGGTCTGGATCAGAATCTATTCTTCAGAAATTTGAATTTAAGATGTCAAATTACAAGATTTTAGTGACATCAAGGTCTGAGTTTCCGAGATTTGGCCCTTCATATCACCTGCAACTATTAGATGATGATAATGCAATGCAACTTTTTCATCACACAGCATTCTTAGGAGACAAGAGCTCTCATATCCCAAACGATCTTGCAAAAAAGATAGTAAAACACTGTAATGGATTTCCACTGGCTATTACAGTGGTAGGAAGATCACTTTGCAGTCAACCTATACAAACGTGGCGAAGTAGGGTAAATGAATGGTCAAAAGGTGCTTCAGTACTCGACTGTGAGATGGAATTGTTTCAATGCCTCCAAAGCAGCTTCGATGCCTTGGATAATGCAACGCCTAAGGTCAAGGATTGTTTGTTAGACCTAGGTGCGTTTCCCGAAGATTCTGAAATTCAAGTTACAGCTCTCATTGATATGTGGGCGGAGTTGTATGATCTAGATGAAGAGATGTCATGCATTGCCAAACTCTATGAACTCAACAATCGCAGTCTAGCTCTTCTACAAGGGGATATGCGGTTGGAAGTTGTACAGGAGGCAATAGAATACAATAGATACGTTGTTAGGCAGCATGATTTGCTCAGAGAACTGGCTATCTACAACTCAAAACGTGATCCGATGGAGCATAGAGAAAGACTGATCATAGAGATATGTGGAAACAATGTTCCCAAATGGTGGAGAGAACAAAAATATCCATACATAAATGCTCGCCTTGTATCTATCTCAACTGATGGAGAGTTCTCAGTGAAACTGCCCGACATGGAATTACCAAAGGCAGAGGTTCTAGTCTTGAATTCTCAATCAGAAAGGTCCTATGCCTTACCGGAATTTGTGGAGAGAATGCATAAACTAAGGATGCTGCTTGTCATAAATTATACCAGAAGGGCAAAATTGGATAATTTTCAACTACTGGGCTCCTTATCAAATCTCAGGACTCTCAGATTAGACGGCATCGATCTTTCTTGCTTAACCAAGAGCCCAGTACAGTTGAAGAATCTACACAAGTTGTATTTGAGAGCATGTGGATATAATAGCAGAGATCTGAAATTATCACATATTATATTTCCAAATCTGAAGGAAATTGTTTTCGAAAGCTGCCTTCTCCTGGAATTCACAGGAGTTCTCTGCGAGCTGATTCATTTAACAAAGCTCAAAATCACTAGTAGATTCCGGTTATCAAACTTGTCTGAAGCCATTGCAAATCTGGTCAATTTAGAAGCGTTGACGCTAGACGTCGGCTTAGTTGCAGGGATTCCTGAATCGATTGGGAATCTCAGGAAATTAAAACTTCTTAAGATTAATAACGATCGGGATTTAGAGAAGTTGCCGGAACAGATGGGTATGCTTAAGTGTCTGAGACATCTCATCATCGAGGGTTGTAGTAGGCTATCTGCGTTGCCTGAATCAATTGGAAATTTGGTGAATCTAGAAGTATTGAGTCTAAGGTATTGTACCCACTTGTCAGAGCTGCCGGAGTCGATCAGAAATCTCCAAAAGTTAAACTTTCTTGACATGTATAGATGCATAGAGATCGAGGAGTTGCCTGAACATTTTGGTGAATTGAAGAGTTTAATGAAGCTGCAAATGGTTGGTTGTTATGCATTGAGAAAACTGCCTGAATCAGTTTCAGATCTTGAGCAGTTAGAGCAAGTGATATGTGTAGATTGGGATGCAACACATCTATGGACACCCTTGTTGCCCACTCTCAAAAACCTTCGCATCGTCGTGCGGGAGGGCAGCAAATCCTTTGTCCCTTGTTGCCCAACTTCCCCTCCAGTTGATATGGTTGAAAAATTTCTAATTGCGTTTGAGAGACTCTTCAGAAAACCCCGGGTGCGCGGCGCACCCGGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

907

Amino Acids

103.46

Weight (kDa)

6.34

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 114 3e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 168 - 324 8.3e-15 NB-ARC domain
LRR_14 PF23598 684 - 769 1.6e-10 Leucine-rich repeat region
LRR_14 PF23598 745 - 862 2.2e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AarI CACCTGC 1 cut(s) 927
AasI GACNNNNNNGTC 1 cut(s) 883
AatII GACGTC 2 cut(s) 612, 2100
Acc36I ACCTGC 2 cut(s) 434, 927
Acc65I GGTACC 1 cut(s) 712
AccB1I GGYRCC 1 cut(s) 712
AccB7I CCANNNNNTGG 2 cut(s) 151, 1539
AccI GTMKAC 1 cut(s) 388
AccII CGCG 1 cut(s) 2703
AciI CCGC 5 cut(s) 5, 1289, 1381, 2593, 2703
AclI AACGTT 1 cut(s) 65
AclWI GGATC 5 cut(s) 136, 182, 195, 826, 1472
AcsI RAATTY 8 cut(s) 839, 845, 1257, 1657, 1691, 1973, 2278, 2657
AcuI CTGAAG 6 cut(s) 518, 818, 1116, 1958, 2073, 2668
AcyI GRCGYC 2 cut(s) 609, 2097
AfaI GTAC 8 cut(s) 156, 287, 293, 714, 1137, 1395, 1848, 2317
AfeI AGCGCT 1 cut(s) 36
AfiI CCNNNNNNNGG 4 cut(s) 151, 902, 1539, 1969
AflII CTTAAG 2 cut(s) 2156, 2210
AflIII ACRYGT 1 cut(s) 2376
AhlI ACTAGT 1 cut(s) 2024
AjnI CCWGG 2 cut(s) 556, 1968
AloI GAACNNNNNNTCC 2 cut(s) 1512, 1544
Alw21I GWGCWC 3 cut(s) 402, 454, 989
Alw26I GTCTC 4 cut(s) 127, 972, 2215, 2671
Alw44I GTGCAC 1 cut(s) 450
AlwI GGATC 5 cut(s) 136, 182, 195, 826, 1472
Ama87I CYCGRG 2 cut(s) 38, 2694
Aor51HI AGCGCT 1 cut(s) 36
AoxI GGCC 2 cut(s) 114, 904
ApaLI GTGCAC 1 cut(s) 450
ApeKI GCWGC 8 cut(s) 431, 1176, 1432, 1723, 1959, 2332, 2443, 2601
ApoI RAATTY 8 cut(s) 839, 845, 1257, 1657, 1691, 1973, 2278, 2657
ArsI GACNNNNNNTTYG 2 cut(s) 2598, 2630
AseI ATTAAT 2 cut(s) 215, 2163
Asp700I GAANNNNTTC 3 cut(s) 828, 1158, 2658
Asp718I GGTACC 1 cut(s) 712
AspA2I CCTAGG 1 cut(s) 1231
AspLEI GCGC 3 cut(s) 37, 2703, 2708
AspS9I GGNCC 2 cut(s) 905, 1674
AsuC2I CCSGG 3 cut(s) 2695, 2696, 2712
AsuHPI GGTGA 5 cut(s) 158, 788, 908, 2296, 2432
AsuII TTCGAA 1 cut(s) 1953
AvaI CYCGRG 2 cut(s) 38, 2694
AvaII GGWCC 1 cut(s) 1674
AvrII CCTAGG 1 cut(s) 1231
AxyI CCTNAGG 2 cut(s) 183, 1206
BaeGI GKGCMC 1 cut(s) 454
BamHI GGATCC 1 cut(s) 187
BanI GGYRCC 1 cut(s) 712
BanII GRGCYC 4 cut(s) 402, 989, 1781, 1844
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
Bbv12I GWGCWC 3 cut(s) 402, 454, 989
BbvI GCAGC 8 cut(s) 443, 1188, 1444, 1710, 1946, 2319, 2430, 2613
BccI CCATC 6 cut(s) 145, 505, 1144, 1477, 1589, 2194
BceAI ACGGC 1 cut(s) 1828
BcgI CGANNNNNNTGC 2 cut(s) 2377, 2411
BciT130I CCWGG 2 cut(s) 558, 1970
BclI TGATCA 1 cut(s) 1503
BcnI CCSGG 3 cut(s) 2695, 2696, 2712
BcoDI GTCTC 4 cut(s) 127, 972, 2215, 2671
BcuI ACTAGT 1 cut(s) 2024
BfaI CTAG 8 cut(s) 122, 1232, 1304, 1358, 1649, 2025, 2093, 2291
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 38
BfrI CTTAAG 2 cut(s) 2156, 2210
BfuAI ACCTGC 2 cut(s) 434, 927
BglII AGATCT 2 cut(s) 1903, 2494
BisI GCNGC 9 cut(s) 432, 1177, 1433, 1724, 1960, 2333, 2444, 2602, 2704
BlnI CCTAGG 1 cut(s) 1231
BlsI GCNGC 9 cut(s) 433, 1178, 1434, 1725, 1961, 2334, 2445, 2603, 2705
BmcAI AGTACT 1 cut(s) 1137
Bme1390I CCNGG 5 cut(s) 558, 1970, 2695, 2696, 2712
Bme18I GGWCC 1 cut(s) 1674
BmeT110I CYCGRG 2 cut(s) 38, 2694
BmgT120I GGNCC 2 cut(s) 905, 1674
BmiI GGNNCC 3 cut(s) 189, 714, 1780
BmrFI CCNGG 5 cut(s) 558, 1970, 2695, 2696, 2712
BmrI ACTGGG 2 cut(s) 1784, 1838
BmsI GCATC 7 cut(s) 1174, 1710, 1824, 2375, 2524, 2592, 2705
BmuI ACTGGG 2 cut(s) 1784, 1838
BpmI CTGGAG 2 cut(s) 540, 2622
Bpu14I TTCGAA 1 cut(s) 1953
BpuEI CTTGAG 3 cut(s) 293, 347, 2519
BpuMI CCSGG 3 cut(s) 2695, 2696, 2712
Bsa29I ATCGAT 2 cut(s) 1818, 2126
BsaBI GATNNNNATC 1 cut(s) 823
BsaHI GRCGYC 2 cut(s) 609, 2097
BsaI GGTCTC 1 cut(s) 127
BsaJI CCNNGG 4 cut(s) 1188, 1231, 2693, 2694
BsaWI WCCGGW 3 cut(s) 172, 1687, 2034
BsaXI ACNNNNNCTCC 4 cut(s) 219, 249, 2620, 2650
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 902, 1539, 1969
Bse1I ACTGG 7 cut(s) 191, 740, 1050, 1458, 1779, 1844, 2639
Bse21I CCTNAGG 2 cut(s) 183, 1206
Bse3DI GCAATG 3 cut(s) 951, 1323, 2058
Bse8I GATNNNNATC 1 cut(s) 823
BseBI CCWGG 2 cut(s) 558, 1970
BseCI ATCGAT 2 cut(s) 1818, 2126
BseDI CCNNGG 4 cut(s) 1188, 1231, 2693, 2694
BseGI GGATG 7 cut(s) 532, 569, 775, 808, 1725, 2539, 2720
BseJI GATNNNNATC 1 cut(s) 823
BseLI CCNNNNNNNGG 4 cut(s) 151, 902, 1539, 1969
BseMI GCAATG 3 cut(s) 951, 1323, 2058
BseMII CTCAG 8 cut(s) 174, 879, 1459, 1620, 1807, 1816, 2152, 2210
BseNI ACTGG 7 cut(s) 191, 740, 1050, 1458, 1779, 1844, 2639
BseRI GAGGAG 1 cut(s) 2414
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 8 cut(s) 443, 1188, 1444, 1710, 1946, 2319, 2430, 2613
Bsh1236I CGCG 1 cut(s) 2703
Bsh1285I CGRYCG 1 cut(s) 2173
BshFI GGCC 2 cut(s) 116, 906
BshNI GGYRCC 1 cut(s) 712
BshVI ATCGAT 2 cut(s) 1818, 2126
BsiEI CGRYCG 1 cut(s) 2173
BsiHKAI GWGCWC 3 cut(s) 402, 454, 989
BsiHKCI CYCGRG 2 cut(s) 38, 2694
BsiSI CCGG 7 cut(s) 173, 1688, 2035, 2192, 2336, 2695, 2712
BslFI GGGAC 1 cut(s) 2600
BslI CCNNNNNNNGG 4 cut(s) 151, 902, 1539, 1969
BsmAI GTCTC 4 cut(s) 127, 972, 2215, 2671
BsmFI GGGAC 1 cut(s) 2600
BsmI GAATGC 2 cut(s) 968, 1710
BsnI GGCC 2 cut(s) 116, 906
Bso31I GGTCTC 1 cut(s) 127
BsoBI CYCGRG 2 cut(s) 38, 2694
Bsp119I TTCGAA 1 cut(s) 1953
Bsp1286I GDGCHC 5 cut(s) 402, 454, 989, 1781, 1844
Bsp1407I TGTACA 1 cut(s) 1393
BspACI CCGC 5 cut(s) 5, 1289, 1381, 2593, 2703
BspANI GGCC 2 cut(s) 116, 906
BspCNI CTCAG 8 cut(s) 175, 880, 1458, 1619, 1806, 1815, 2151, 2211
BspDI ATCGAT 2 cut(s) 1818, 2126
BspFNI CGCG 1 cut(s) 2703
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 3 cut(s) 189, 714, 1780
BspMAI CTGCAG 1 cut(s) 433
BspMI ACCTGC 2 cut(s) 434, 927
BspPI GGATC 5 cut(s) 136, 182, 195, 826, 1472
BspQI GCTCTTC 1 cut(s) 1368
BspT104I TTCGAA 1 cut(s) 1953
BspT107I GGYRCC 1 cut(s) 712
BspTI CTTAAG 2 cut(s) 2156, 2210
BspTNI GGTCTC 1 cut(s) 127
BsrDI GCAATG 3 cut(s) 951, 1323, 2058
BsrGI TGTACA 1 cut(s) 1393
BsrI ACTGG 7 cut(s) 191, 740, 1050, 1458, 1779, 1844, 2639
BssECI CCNNGG 4 cut(s) 1188, 1231, 2693, 2694
BssNAI GTATAC 1 cut(s) 389
BssNI GRCGYC 2 cut(s) 609, 2097
BssT1I CCWWGG 2 cut(s) 1188, 1231
Bst1107I GTATAC 1 cut(s) 389
Bst2UI CCWGG 2 cut(s) 558, 1970
Bst4CI ACNGT 7 cut(s) 48, 468, 649, 1031, 1057, 1145, 1851
Bst6I CTCTTC 4 cut(s) 1305, 1368, 2423, 2687
BstACI GRCGYC 2 cut(s) 609, 2097
BstAFI CTTAAG 2 cut(s) 2156, 2210
BstAPI GCANNNNNTGC 2 cut(s) 449, 1441
BstAUI TGTACA 1 cut(s) 1393
BstBI TTCGAA 1 cut(s) 1953
BstC8I GCNNGC 2 cut(s) 1573, 1994
BstF5I GGATG 7 cut(s) 532, 569, 775, 808, 1725, 2539, 2720
BstFNI CGCG 1 cut(s) 2703
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 3 cut(s) 37, 2703, 2708
BstMAI GTCTC 4 cut(s) 127, 972, 2215, 2671
BstMCI CGRYCG 1 cut(s) 2173
BstMWI GCNNNNNNNGC 5 cut(s) 440, 449, 1173, 1441, 2509
BstNI CCWGG 2 cut(s) 558, 1970
BstNSI RCATGY 2 cut(s) 1887, 2380
BstSCI CCNGG 5 cut(s) 556, 1968, 2693, 2694, 2710
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 454
BstUI CGCG 1 cut(s) 2703
BstV1I GCAGC 8 cut(s) 443, 1188, 1444, 1710, 1946, 2319, 2430, 2613
BstX2I RGATCY 3 cut(s) 187, 1903, 2494
BstXI CCANNNNNNTGG 1 cut(s) 747
BstYI RGATCY 3 cut(s) 187, 1903, 2494
BstZ17I GTATAC 1 cut(s) 389
Bsu15I ATCGAT 2 cut(s) 1818, 2126
Bsu36I CCTNAGG 2 cut(s) 183, 1206
BsuRI GGCC 2 cut(s) 116, 906
BsuTUI ATCGAT 2 cut(s) 1818, 2126
BtsCI GGATG 7 cut(s) 532, 569, 775, 808, 1725, 2539, 2720
BtsI GCAGTG 2 cut(s) 237, 536
BtsIMutI CAGTG 9 cut(s) 237, 494, 536, 645, 747, 1027, 1043, 1062, 1614
BveI ACCTGC 2 cut(s) 434, 927
Cac8I GCNNGC 2 cut(s) 1573, 1994
CciI TCATGA 1 cut(s) 678
CfoI GCGC 3 cut(s) 37, 2703, 2708
Cfr13I GGNCC 2 cut(s) 905, 1674
Cfr9I CCCGGG 1 cut(s) 2694
ClaI ATCGAT 2 cut(s) 1818, 2126
CseI GACGC 2 cut(s) 134, 2098
Csp6I GTAC 8 cut(s) 155, 286, 292, 713, 1136, 1394, 1847, 2316
CviAII CATG 7 cut(s) 395, 679, 1320, 1436, 1624, 1884, 2377
CviQI GTAC 8 cut(s) 155, 286, 292, 713, 1136, 1394, 1847, 2316
DrdI GACNNNNNNGTC 1 cut(s) 883
DseDI GACNNNNNNGTC 1 cut(s) 883
Eam1104I CTCTTC 4 cut(s) 1305, 1368, 2423, 2687
EarI CTCTTC 4 cut(s) 1305, 1368, 2423, 2687
EciI GGCGGA 1 cut(s) 1304
Ecl136II GAGCTC 2 cut(s) 400, 987
Eco130I CCWWGG 2 cut(s) 1188, 1231
Eco24I GRGCYC 4 cut(s) 402, 989, 1781, 1844
Eco31I GGTCTC 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 1674
Eco47III AGCGCT 1 cut(s) 36
Eco53kI GAGCTC 2 cut(s) 400, 987
Eco57I CTGAAG 6 cut(s) 518, 818, 1116, 1958, 2073, 2668
Eco81I CCTNAGG 2 cut(s) 183, 1206
Eco88I CYCGRG 2 cut(s) 38, 2694
EcoICRI GAGCTC 2 cut(s) 400, 987
EcoO109I RGGNCCY 1 cut(s) 1674
EcoRI GAATTC 2 cut(s) 1657, 1973
EcoRII CCWGG 2 cut(s) 556, 1968
EcoT14I CCWWGG 2 cut(s) 1188, 1231
EcoT22I ATGCAT 4 cut(s) 1325, 1710, 2390, 2467
EcoT38I GRGCYC 4 cut(s) 402, 989, 1781, 1844
ErhI CCWWGG 2 cut(s) 1188, 1231
FaeI CATG 7 cut(s) 398, 682, 1323, 1439, 1627, 1887, 2380
FalI AAGNNNNNCTT 2 cut(s) 2351, 2383
FaqI GGGAC 1 cut(s) 2600
FatI CATG 7 cut(s) 394, 678, 1319, 1435, 1623, 1883, 2376
FauI CCCGC 1 cut(s) 2586
FbaI TGATCA 1 cut(s) 1503
FblI GTMKAC 1 cut(s) 388
Fnu4HI GCNGC 9 cut(s) 432, 1177, 1433, 1724, 1960, 2333, 2444, 2602, 2704
FokI GGATG 6 cut(s) 539, 576, 782, 815, 1732, 2546
FriOI GRGCYC 4 cut(s) 402, 989, 1781, 1844
Fsp4HI GCNGC 9 cut(s) 432, 1177, 1433, 1724, 1960, 2333, 2444, 2602, 2704
FspBI CTAG 8 cut(s) 122, 1232, 1304, 1358, 1649, 2025, 2093, 2291
GlaI GCGC 3 cut(s) 36, 2702, 2707
GluI GCNGC 9 cut(s) 432, 1177, 1433, 1724, 1960, 2333, 2444, 2602, 2704
GsuI CTGGAG 2 cut(s) 540, 2622
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 2 cut(s) 116, 906
HapII CCGG 7 cut(s) 173, 1688, 2035, 2192, 2336, 2695, 2712
HgaI GACGC 2 cut(s) 134, 2098
HhaI GCGC 3 cut(s) 37, 2703, 2708
Hin1I GRCGYC 2 cut(s) 609, 2097
Hin1II CATG 7 cut(s) 398, 682, 1323, 1439, 1627, 1887, 2380
Hin6I GCGC 3 cut(s) 35, 2701, 2706
HinP1I GCGC 3 cut(s) 35, 2701, 2706
HincII GTYRAC 2 cut(s) 1082, 2088
HindII GTYRAC 2 cut(s) 1082, 2088
HpaII CCGG 7 cut(s) 173, 1688, 2035, 2192, 2336, 2695, 2712
HphI GGTGA 5 cut(s) 158, 788, 908, 2296, 2432
Hpy166II GTNNAC 6 cut(s) 294, 344, 389, 452, 1082, 2088
Hpy8I GTNNAC 6 cut(s) 294, 344, 389, 452, 1082, 2088
Hpy99I CGWCG 2 cut(s) 2102, 2591
HpyAV CCTTC 9 cut(s) 218, 493, 760, 767, 918, 1740, 1933, 1973, 2588
HpyCH4III ACNGT 7 cut(s) 48, 468, 649, 1031, 1057, 1145, 1851
HpyCH4IV ACGT 7 cut(s) 65, 160, 609, 1094, 1422, 1474, 2097
HpyF10VI GCNNNNNNNGC 5 cut(s) 440, 449, 1173, 1441, 2509
HpySE526I ACGT 7 cut(s) 65, 160, 609, 1094, 1422, 1474, 2097
Hsp92I GRCGYC 2 cut(s) 609, 2097
Hsp92II CATG 7 cut(s) 398, 682, 1323, 1439, 1627, 1887, 2380
HspAI GCGC 3 cut(s) 35, 2701, 2706
KpnI GGTACC 1 cut(s) 716
Ksp22I TGATCA 1 cut(s) 1503
LguI GCTCTTC 1 cut(s) 1368
LmnI GCTCC 5 cut(s) 227, 559, 670, 1486, 1784
Lsp1109I GCAGC 8 cut(s) 443, 1188, 1444, 1710, 1946, 2319, 2430, 2613
LweI GCATC 7 cut(s) 1174, 1710, 1824, 2375, 2524, 2592, 2705
MaeI CTAG 8 cut(s) 122, 1232, 1304, 1358, 1649, 2025, 2093, 2291
MaeII ACGT 7 cut(s) 65, 160, 609, 1094, 1422, 1474, 2097
MaeIII GTNAC 6 cut(s) 42, 367, 468, 643, 874, 1264
MfeI CAATTG 2 cut(s) 435, 2271
MflI RGATCY 3 cut(s) 187, 1903, 2494
MhlI GDGCHC 5 cut(s) 402, 454, 989, 1781, 1844
MlyI GAGTC 4 cut(s) 1792, 2311, 2348, 2673
MmeI TCCRAC 3 cut(s) 460, 648, 1365
Mph1103I ATGCAT 4 cut(s) 1325, 1710, 2390, 2467
MroXI GAANNNNTTC 3 cut(s) 828, 1158, 2658
MslI CAYNNNNRTG 6 cut(s) 1032, 1280, 2418, 2545, 2588, 2713
MspCI CTTAAG 2 cut(s) 2156, 2210
MspI CCGG 7 cut(s) 173, 1688, 2035, 2192, 2336, 2695, 2712
MspR9I CCNGG 5 cut(s) 558, 1970, 2695, 2696, 2712
MteI GCGCNGCGC 1 cut(s) 2704
MunI CAATTG 2 cut(s) 435, 2271
Mva1269I GAATGC 2 cut(s) 968, 1710
MvaI CCWGG 2 cut(s) 558, 1970
MvnI CGCG 1 cut(s) 2703
MwoI GCNNNNNNNGC 5 cut(s) 440, 449, 1173, 1441, 2509
NciI CCSGG 3 cut(s) 2695, 2696, 2712
NlaIII CATG 7 cut(s) 398, 682, 1323, 1439, 1627, 1887, 2380
NlaIV GGNNCC 3 cut(s) 189, 714, 1780
NmuCI GTSAC 4 cut(s) 367, 468, 643, 874
NsiI ATGCAT 4 cut(s) 1325, 1710, 2390, 2467
NspI RCATGY 2 cut(s) 1887, 2380
NspV TTCGAA 1 cut(s) 1953
PaeR7I CTCGAG 1 cut(s) 38
PagI TCATGA 1 cut(s) 678
PaqCI CACCTGC 1 cut(s) 927
PciI ACATGT 1 cut(s) 2376
PciSI GCTCTTC 1 cut(s) 1368
PctI GAATGC 2 cut(s) 968, 1710
PdmI GAANNNNTTC 3 cut(s) 828, 1158, 2658
PflMI CCANNNNNTGG 2 cut(s) 151, 1539
PfoI TCCNGGA 2 cut(s) 556, 1968
PkrI GCNGC 9 cut(s) 433, 1178, 1434, 1725, 1961, 2334, 2445, 2603, 2705
Ple19I CGATCG 1 cut(s) 2173
PleI GAGTC 4 cut(s) 1792, 2310, 2347, 2673
PpsI GAGTC 4 cut(s) 1792, 2310, 2347, 2673
PpuMI RGGWCCY 1 cut(s) 1674
PscI ACATGT 1 cut(s) 2376
PshBI ATTAAT 2 cut(s) 215, 2163
PsiI TTATAA 1 cut(s) 708
Psp124BI GAGCTC 2 cut(s) 402, 989
Psp1406I AACGTT 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 1674
Psp6I CCWGG 2 cut(s) 556, 1968
PspGI CCWGG 2 cut(s) 556, 1968
PspN4I GGNNCC 3 cut(s) 189, 714, 1780
PspPI GGNCC 2 cut(s) 905, 1674
PspPPI RGGWCCY 1 cut(s) 1674
PspXI VCTCGAGB 1 cut(s) 38
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 3 cut(s) 187, 1903, 2494
PvuI CGATCG 1 cut(s) 2173
RsaI GTAC 8 cut(s) 156, 287, 293, 714, 1137, 1395, 1848, 2317
RsaNI GTAC 8 cut(s) 155, 286, 292, 713, 1136, 1394, 1847, 2316
RseI CAYNNNNRTG 6 cut(s) 1032, 1280, 2418, 2545, 2588, 2713
SacI GAGCTC 2 cut(s) 402, 989
SapI GCTCTTC 1 cut(s) 1368
SatI GCNGC 9 cut(s) 432, 1177, 1433, 1724, 1960, 2333, 2444, 2602, 2704
Sau96I GGNCC 2 cut(s) 905, 1674
ScaI AGTACT 1 cut(s) 1137
SchI GAGTC 4 cut(s) 1792, 2311, 2348, 2673
ScrFI CCNGG 5 cut(s) 558, 1970, 2695, 2696, 2712
SduI GDGCHC 5 cut(s) 402, 454, 989, 1781, 1844
SfaNI GCATC 7 cut(s) 1174, 1710, 1824, 2375, 2524, 2592, 2705
SfcI CTRYAG 1 cut(s) 429
Sfr274I CTCGAG 1 cut(s) 38
SfuI TTCGAA 1 cut(s) 1953
SinI GGWCC 1 cut(s) 1674
SlaI CTCGAG 1 cut(s) 38
SmaI CCCGGG 1 cut(s) 2696
SmiMI CAYNNNNRTG 6 cut(s) 1032, 1280, 2418, 2545, 2588, 2713
SmlI CTYRAG 6 cut(s) 38, 308, 326, 2156, 2210, 2498
SmoI CTYRAG 6 cut(s) 38, 308, 326, 2156, 2210, 2498
SpeI ACTAGT 1 cut(s) 2024
SsiI CCGC 5 cut(s) 5, 1289, 1381, 2593, 2703
SspMI CTAG 8 cut(s) 122, 1232, 1304, 1358, 1649, 2025, 2093, 2291
SstI GAGCTC 2 cut(s) 402, 989
StyD4I CCNGG 5 cut(s) 556, 1968, 2693, 2694, 2710
StyI CCWWGG 2 cut(s) 1188, 1231
TaaI ACNGT 7 cut(s) 48, 468, 649, 1031, 1057, 1145, 1851
TaiI ACGT 7 cut(s) 68, 163, 612, 1097, 1425, 1477, 2100
TaqI TCGA 9 cut(s) 39, 1140, 1182, 1818, 1953, 2126, 2235, 2342, 2397
TaqII GACCGA 1 cut(s) 151
TatI WGTACW 5 cut(s) 285, 291, 1135, 1393, 1846
TauI GCSGC 1 cut(s) 2706
TscAI CASTG 9 cut(s) 237, 501, 543, 652, 747, 1034, 1050, 1062, 1614
TseFI GTSAC 4 cut(s) 367, 468, 643, 874
TseI GCWGC 8 cut(s) 431, 1176, 1432, 1723, 1959, 2332, 2443, 2601
Tsp45I GTSAC 4 cut(s) 367, 468, 643, 874
TspMI CCCGGG 1 cut(s) 2694
TspRI CASTG 9 cut(s) 237, 501, 543, 652, 747, 1034, 1050, 1062, 1614
Van91I CCANNNNNTGG 2 cut(s) 151, 1539
Vha464I CTTAAG 2 cut(s) 2156, 2210
VneI GTGCAC 1 cut(s) 450
VpaK11BI GGWCC 1 cut(s) 1674
VspI ATTAAT 2 cut(s) 215, 2163
XapI RAATTY 8 cut(s) 839, 845, 1257, 1657, 1691, 1973, 2278, 2657
XbaI TCTAGA 2 cut(s) 1303, 2290
XceI RCATGY 2 cut(s) 1887, 2380
XcmI CCANNNNNNNNNTGG 1 cut(s) 2065
XhoI CTCGAG 1 cut(s) 38
XmaI CCCGGG 1 cut(s) 2694
XmaJI CCTAGG 1 cut(s) 1231
XmiI GTMKAC 1 cut(s) 388
XmnI GAANNNNTTC 3 cut(s) 828, 1158, 2658
XspI CTAG 8 cut(s) 122, 1232, 1304, 1358, 1649, 2025, 2093, 2291
ZraI GACGTC 2 cut(s) 610, 2098
ZrmI AGTACT 1 cut(s) 1137
Zsp2I ATGCAT 4 cut(s) 1325, 1710, 2390, 2467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.