MD15G1277700.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000039
Physical Location & Seq
Forward (+)
24447859 .. 24451158
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1277700.v1.1.491

Sequence Viewer

Length: 2427 bp
ATGTCTCTGGCTTCAGTCGGAATGGGTGCTCTGGGAACGTCATTTCAAATGCTGTTTGATGCTGTAAAATCAGTGCAGGAGGAGAATACAATGTTCCAACACCTCCTCGGAGACATCAAATCCACACTGAACTCTCTACAACCACTCATCAAAGACATCGAAAAGTATAACTCCAAGGAGGGACTGGAAAATTATGCAATACAGATGGAGGAAGGAGCAAAGCTTGTTCAGAAGTGCAGCAAAGTAGGCGTGTGGAGAAAACACACATACACCAAGAAGCTTAGTCAACTGGACAAATCTCTCCGAAGAGTGTTTGATGTGCTTAAACGGCAGGGTATGAGGGATGCGAGGGAGACGTTAGTTTCTTTGAGGAGTATCCAGACGGTGCTCTCTCGAATAGAAGAGAGTTTGAAGATGAAAAATAATCAGCCTGAAATGGTTAAATGTTCGTCTGCGGTGCCTGAAGCGCCATCAGTTACAGTCGGGTTAGATTTACCGTTGAAGGAGTTGAAGATGAAGCTCCTTAAGGATGAACAGGTGTCTATGCTTGTGCTGACTGCTGCTGGAGGATGCGGGAAAACCACCTTGGCTAAAATGTTTTGTCAAGATCAAGAAGTCAAAGATACATTCAAGAACAACATCTTCTTTGTCAATGTCTCGAAAAAGCCCAACTTGGACCTTATTGTACATGAACTATATCAACGGAAGGGGTTCGAGCTGCCTGCTTTCGAAAACGAAGTCATGGCAGCTAACTGGTTGCAACAATTTCTGAAGGAATCAAGACAAAATCCTCTACTGTTTGTCCTGGATGATGTTTGGTCAGGATCAGAGTCCCTTCTTGAGAAGTTTGATCAATTCAAGTTTTCAAATTGCAAGATTTTGGTCACTTCAAGATTTGCATTTCCAAGATTCGGTTCTCCTTATCATTTGGCACCATTGAATGATGAAGATGCAATGGCTCTTTTTCAGTATTCTGCATCCTCGGAGGATAAGAGCTCTTATACTTATGAAGATCCTTCGAGAAAGATTATCAAGCGCTGTAAGGGATGCCCACTTGCCATTGCATTGGTCGGGAGATCACTTCGCGGCCAGCCTATAGAAATCTGGCACAAAAGAGTAGTGGAATGGTGCAAAGGTTCATCTATTCTTGATTCTGACAAGGATTTACTTGCTTGCCTCCAAAGCAGCTTAGATGCCTTGGATAAAGAGAAGGCTATCATCAAGGAATGTTTCCTCGATCTAGGTTCTTTTCCGGAGGACCAAAGAATTGCTGCTGCCACCCTCATTGATATGTGGGCGGAGTTATATGGTCTAGATGAAGAAATTCTGACCATTGCCAACCTTCACGAGCTCACCACCCGAAGTTTAGCTAATCTTGTTGTCACAAGACATGAGGGGGAGACAGATGGCTATTACGCTGAACACTTTGTCACCCTGCACGGCATGCTTAGAGAGTTGGCGATCCATCAGGCGAGTCAAGACCCAATAGAACATAGGAAACGACTATTTTTAAATATCTGTGGGAACAAACTTCCTAAGTGGTTGACAGAACAAAAGTATCAAGCGATCAAGGCTGGGCTATTATCTATCTCAACAGATGGAGCCTTCTCAGCAAAATGGCCAAACATGCAAGTACCCGAAGCAGAGGTTCTAGTTCTGAATTTTCAAACAAAGAACTATGCCTTACCTGAGTTTGTGGAGAAAATGGATAAACTAAAGGTTCTAATACTAAATAATTACGGTATGTTACCTGCTGAACTAGATAATTTTGAACTACTTGGTTCGCTATCAAATCTTAAGAGAATTAGATTAGAACGTATTTCAATTCCTCCCATAAGCAAGGTCCTTAAACAGTTGAAAAGCTTGCAAAAGATCTCTCTGTTCATGTGTGATATCGGCCAAGGTTTCGTCAAAAATTTAGATGCACTGCCAAATCTAGTGGAATTGAACATTGATTATTGCCATGATCTGGTGCAACTTCCTCCCAACCTTTGTGACCTTGTTCACCTTAAGAAGCTTAGCATCACCAACTGTCATAAGCTTTCTGCCTTGCCTGCAGACATCGGAAAGCTAGTCAATCTTGAAGTGTTGAGGCTTAGGTCATGTGCAGATTTGTTAGAGTTGCCAGGCTCGATTAGGAACCTTAAAGTTTTAAAATTTCTCGACATATCTGATTGCTTTAGCGTAAAGGAGTTGCCAGAGGACATTGGTGCAATGTGCAGCATGGAAAAGATCAACGTGAGCCAGTGTTCTAGACTGAAAGGGCTGCCTGCTTCTGTTATGGATCTTGAGCAGTTGAACGAAGTGATATGCGACGAGGAGACGAAAACTTTATGGGAACCCTTCTTGCCAATTCTCACAAACATGCGCATTAAGGTGATCAAAGAAGATATCAACCTAAATTGGCTCAACAAGCTTCCACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

809

Amino Acids

91.51

Weight (kDa)

6.58

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 130 1.1e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 167 - 299 2.2e-14 NB-ARC domain
AAA_22 PF13401 181 - 283 3.8e-06 AAA domain
LRR_14 PF23598 662 - 768 1.5e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2369
Acc36I ACCTGC 1 cut(s) 1759
AccB1I GGYRCC 2 cut(s) 457, 931
AccB7I CCANNNNNTGG 1 cut(s) 1969
AccII CGCG 1 cut(s) 1086
AccIII TCCGGA 1 cut(s) 1252
AciI CCGC 4 cut(s) 455, 573, 1086, 1298
AclWI GGATC 4 cut(s) 832, 1007, 1456, 2292
AcoI YGGCCR 3 cut(s) 1087, 1619, 1897
AcsI RAATTY 4 cut(s) 1323, 1660, 1915, 2156
AcuI CTGAAG 2 cut(s) 483, 791
AfaI GTAC 2 cut(s) 687, 1635
AfeI AGCGCT 1 cut(s) 1037
AfiI CCNNNNNNNGG 2 cut(s) 911, 1969
AflII CTTAAG 3 cut(s) 524, 1796, 2009
AjnI CCWGG 2 cut(s) 804, 2125
AjuI GAANNNNNNNTTGG 4 cut(s) 569, 601, 898, 930
Alw21I GWGCWC 4 cut(s) 31, 390, 998, 1353
Alw26I GTCTC 6 cut(s) 9, 105, 347, 661, 1394, 2315
AlwI GGATC 4 cut(s) 832, 1007, 1456, 2292
Aor13HI TCCGGA 1 cut(s) 1252
Aor51HI AGCGCT 1 cut(s) 1037
AoxI GGCC 3 cut(s) 1087, 1619, 1897
ApeKI GCWGC 9 cut(s) 237, 560, 718, 746, 1185, 1271, 1274, 2220, 2266
ApoI RAATTY 4 cut(s) 1323, 1660, 1915, 2156
ArsI GACNNNNNNTTYG 2 cut(s) 723, 755
Asp700I GAANNNNTTC 2 cut(s) 710, 1323
AspLEI GCGC 3 cut(s) 469, 1038, 2370
AspS9I GGNCC 3 cut(s) 676, 1258, 1843
AsuHPI GGTGA 5 cut(s) 1345, 1423, 1997, 2017, 2389
AsuII TTCGAA 1 cut(s) 729
AvaII GGWCC 3 cut(s) 676, 1258, 1843
BaeI ACNNNNGTAYC 2 cut(s) 1541, 1574
BalI TGGCCA 1 cut(s) 1621
BanI GGYRCC 2 cut(s) 457, 931
BanII GRGCYC 2 cut(s) 998, 1353
BauI CACGAG 1 cut(s) 1346
Bbv12I GWGCWC 4 cut(s) 31, 390, 998, 1353
BbvI GCAGC 9 cut(s) 249, 547, 705, 758, 1197, 1258, 1261, 2232, 2253
BccI CCATC 5 cut(s) 199, 478, 1400, 1473, 1592
BceAI ACGGC 2 cut(s) 344, 1456
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 2 cut(s) 806, 2127
BciVI GTATCC 1 cut(s) 386
BclI TGATCA 2 cut(s) 850, 2379
BcoDI GTCTC 6 cut(s) 9, 105, 347, 661, 1394, 2315
BfaI CTAG 7 cut(s) 1241, 1313, 1652, 1760, 1937, 2072, 2253
BfmI CTRYAG 2 cut(s) 1095, 2055
BfoI RGCGCY 2 cut(s) 470, 1039
BfrI CTTAAG 3 cut(s) 524, 1796, 2009
BfuAI ACCTGC 1 cut(s) 1759
BfuI GTATCC 1 cut(s) 386
BglII AGATCT 1 cut(s) 1872
BlpI GCTNAGC 1 cut(s) 2018
Bme1390I CCNGG 2 cut(s) 806, 2127
Bme18I GGWCC 3 cut(s) 676, 1258, 1843
BmgT120I GGNCC 3 cut(s) 676, 1258, 1843
BmiI GGNNCC 5 cut(s) 459, 933, 1603, 2141, 2340
BmrFI CCNGG 2 cut(s) 806, 2127
BmsI GCATC 9 cut(s) 49, 334, 560, 940, 986, 1037, 1183, 1912, 2031
BpmI CTGGAG 1 cut(s) 585
Bpu10I CCTNAGC 1 cut(s) 2096
Bpu1102I GCTNAGC 1 cut(s) 2018
Bpu14I TTCGAA 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 860, 2309
BsaJI CCNNGG 6 cut(s) 106, 174, 585, 981, 1197, 1900
BsaWI WCCGGW 1 cut(s) 1252
Bsc4I CCNNNNNNNGG 2 cut(s) 911, 1969
Bse1I ACTGG 4 cut(s) 189, 294, 758, 2245
Bse3DI GCAATG 4 cut(s) 960, 1059, 1332, 2220
BseAI TCCGGA 1 cut(s) 1252
BseBI CCWGG 2 cut(s) 806, 2127
BseDI CCNNGG 6 cut(s) 106, 174, 585, 981, 1197, 1900
BseGI GGATG 6 cut(s) 349, 535, 575, 814, 977, 1052
BseLI CCNNNNNNNGG 2 cut(s) 911, 1969
BseMI GCAATG 4 cut(s) 960, 1059, 1332, 2220
BseMII CTCAG 2 cut(s) 1623, 1680
BseNI ACTGG 4 cut(s) 189, 294, 758, 2245
BseRI GAGGAG 4 cut(s) 95, 95, 385, 2333
BseXI GCAGC 9 cut(s) 249, 547, 705, 758, 1197, 1258, 1261, 2232, 2253
BseYI CCCAGC 1 cut(s) 1574
BsgI GTGCAG 5 cut(s) 95, 256, 1421, 2127, 2239
Bsh1236I CGCG 1 cut(s) 1086
BshFI GGCC 3 cut(s) 1089, 1621, 1899
BshNI GGYRCC 2 cut(s) 457, 931
BsiHKAI GWGCWC 4 cut(s) 31, 390, 998, 1353
BsiSI CCGG 1 cut(s) 1253
BslFI GGGAC 2 cut(s) 195, 817
BslI CCNNNNNNNGG 2 cut(s) 911, 1969
BsmAI GTCTC 6 cut(s) 9, 105, 347, 661, 1394, 2315
BsmBI CGTCTC 2 cut(s) 347, 2315
BsmFI GGGAC 2 cut(s) 195, 817
BsnI GGCC 3 cut(s) 1089, 1621, 1899
Bsp119I TTCGAA 1 cut(s) 729
Bsp1286I GDGCHC 4 cut(s) 31, 390, 998, 1353
Bsp13I TCCGGA 1 cut(s) 1252
Bsp1407I TGTACA 1 cut(s) 685
Bsp1720I GCTNAGC 1 cut(s) 2018
BspACI CCGC 4 cut(s) 455, 573, 1086, 1298
BspANI GGCC 3 cut(s) 1089, 1621, 1899
BspCNI CTCAG 2 cut(s) 1622, 1681
BspEI TCCGGA 1 cut(s) 1252
BspFNI CGCG 1 cut(s) 1086
BspLI GGNNCC 5 cut(s) 459, 933, 1603, 2141, 2340
BspMAI CTGCAG 1 cut(s) 2059
BspMI ACCTGC 1 cut(s) 1759
BspPI GGATC 4 cut(s) 832, 1007, 1456, 2292
BspT104I TTCGAA 1 cut(s) 729
BspT107I GGYRCC 2 cut(s) 457, 931
BspTI CTTAAG 3 cut(s) 524, 1796, 2009
BsrDI GCAATG 4 cut(s) 960, 1059, 1332, 2220
BsrGI TGTACA 1 cut(s) 685
BsrI ACTGG 4 cut(s) 189, 294, 758, 2245
BssECI CCNNGG 6 cut(s) 106, 174, 585, 981, 1197, 1900
BssSI CACGAG 1 cut(s) 1346
BssT1I CCWWGG 4 cut(s) 174, 585, 1197, 1900
Bst2BI CACGAG 1 cut(s) 1346
Bst2UI CCWGG 2 cut(s) 806, 2127
Bst4CI ACNGT 7 cut(s) 385, 481, 498, 798, 1742, 1854, 2033
Bst6I CTCTTC 2 cut(s) 301, 396
BstAFI CTTAAG 3 cut(s) 524, 1796, 2009
BstAPI GCANNNNNTGC 1 cut(s) 1444
BstAUI TGTACA 1 cut(s) 685
BstBI TTCGAA 1 cut(s) 729
BstC8I GCNNGC 7 cut(s) 723, 1091, 1174, 1445, 1865, 2055, 2271
BstDEI CTNAG 8 cut(s) 281, 1189, 1448, 1536, 1609, 1689, 2018, 2096
BstF5I GGATG 6 cut(s) 349, 535, 575, 814, 977, 1052
BstFNI CGCG 1 cut(s) 1086
BstH2I RGCGCY 2 cut(s) 470, 1039
BstHHI GCGC 3 cut(s) 469, 1038, 2370
BstMAI GTCTC 6 cut(s) 9, 105, 347, 661, 1394, 2315
BstNI CCWGG 2 cut(s) 806, 2127
BstNSI RCATGY 3 cut(s) 1447, 1630, 2368
BstSCI CCNGG 2 cut(s) 804, 2125
BstSFI CTRYAG 2 cut(s) 1095, 2055
BstUI CGCG 1 cut(s) 1086
BstV1I GCAGC 9 cut(s) 249, 547, 705, 758, 1197, 1258, 1261, 2232, 2253
BstX2I RGATCY 3 cut(s) 1012, 1872, 2284
BstXI CCANNNNNNTGG 1 cut(s) 1066
BstYI RGATCY 3 cut(s) 1012, 1872, 2284
BsuI GTATCC 1 cut(s) 386
BsuRI GGCC 3 cut(s) 1089, 1621, 1899
BtsCI GGATG 6 cut(s) 349, 535, 575, 814, 977, 1052
BtsI GCAGTG 1 cut(s) 1925
BtsIMutI CAGTG 4 cut(s) 78, 125, 1925, 2252
BveI ACCTGC 1 cut(s) 1759
Cac8I GCNNGC 7 cut(s) 723, 1091, 1174, 1445, 1865, 2055, 2271
CfoI GCGC 3 cut(s) 469, 1038, 2370
Cfr13I GGNCC 3 cut(s) 676, 1258, 1843
Csp6I GTAC 2 cut(s) 686, 1634
CspCI CAANNNNNGTGG 4 cut(s) 1267, 1302, 1920, 1955
CviQI GTAC 2 cut(s) 686, 1634
DdeI CTNAG 8 cut(s) 281, 1189, 1448, 1536, 1609, 1689, 2018, 2096
DraI TTTAAA 2 cut(s) 1512, 2154
EaeI YGGCCR 3 cut(s) 1087, 1619, 1897
Eam1104I CTCTTC 2 cut(s) 301, 396
EarI CTCTTC 2 cut(s) 301, 396
EciI GGCGGA 1 cut(s) 1313
Ecl136II GAGCTC 2 cut(s) 996, 1351
Eco130I CCWWGG 4 cut(s) 174, 585, 1197, 1900
Eco24I GRGCYC 2 cut(s) 998, 1353
Eco32I GATATC 2 cut(s) 1894, 2392
Eco47I GGWCC 3 cut(s) 676, 1258, 1843
Eco47III AGCGCT 1 cut(s) 1037
Eco53kI GAGCTC 2 cut(s) 996, 1351
Eco57I CTGAAG 2 cut(s) 483, 791
EcoICRI GAGCTC 2 cut(s) 996, 1351
EcoO109I RGGNCCY 1 cut(s) 1843
EcoRII CCWGG 2 cut(s) 804, 2125
EcoRV GATATC 2 cut(s) 1894, 2392
EcoT14I CCWWGG 4 cut(s) 174, 585, 1197, 1900
EcoT38I GRGCYC 2 cut(s) 998, 1353
ErhI CCWWGG 4 cut(s) 174, 585, 1197, 1900
Esp3I CGTCTC 2 cut(s) 347, 2315
FalI AAGNNNNNCTT 2 cut(s) 656, 688
FaqI GGGAC 2 cut(s) 195, 817
FauI CCCGC 1 cut(s) 566
FbaI TGATCA 2 cut(s) 850, 2379
FokI GGATG 6 cut(s) 356, 542, 582, 821, 964, 1059
FriOI GRGCYC 2 cut(s) 998, 1353
FspAI RTGCGCAY 1 cut(s) 2369
FspBI CTAG 7 cut(s) 1241, 1313, 1652, 1760, 1937, 2072, 2253
FspI TGCGCA 1 cut(s) 2369
GlaI GCGC 3 cut(s) 468, 1037, 2369
GsaI CCCAGC 1 cut(s) 1578
GsuI CTGGAG 1 cut(s) 585
HaeII RGCGCY 2 cut(s) 470, 1039
HaeIII GGCC 3 cut(s) 1089, 1621, 1899
HapII CCGG 1 cut(s) 1253
HhaI GCGC 3 cut(s) 469, 1038, 2370
Hin6I GCGC 3 cut(s) 467, 1036, 2368
HinP1I GCGC 3 cut(s) 467, 1036, 2368
HincII GTYRAC 2 cut(s) 287, 1545
HindII GTYRAC 2 cut(s) 287, 1545
HindIII AAGCTT 6 cut(s) 221, 278, 1861, 2015, 2039, 2414
HinfI GANTC 5 cut(s) 776, 830, 909, 1151, 1474
HpaII CCGG 1 cut(s) 1253
HphI GGTGA 5 cut(s) 1345, 1423, 1997, 2017, 2389
Hpy166II GTNNAC 3 cut(s) 287, 1545, 2005
Hpy8I GTNNAC 3 cut(s) 287, 1545, 2005
Hpy99I CGWCG 1 cut(s) 2318
HpyCH4III ACNGT 7 cut(s) 385, 481, 498, 798, 1742, 1854, 2033
HpyCH4IV ACGT 4 cut(s) 38, 356, 1816, 2238
HpyF3I CTNAG 8 cut(s) 281, 1189, 1448, 1536, 1609, 1689, 2018, 2096
HpySE526I ACGT 4 cut(s) 38, 356, 1816, 2238
HspAI GCGC 3 cut(s) 467, 1036, 2368
Kpn2I TCCGGA 1 cut(s) 1252
Ksp22I TGATCA 2 cut(s) 850, 2379
LmnI GCTCC 3 cut(s) 215, 525, 1601
Lsp1109I GCAGC 9 cut(s) 249, 547, 705, 758, 1197, 1258, 1261, 2232, 2253
LweI GCATC 9 cut(s) 49, 334, 560, 940, 986, 1037, 1183, 1912, 2031
MaeI CTAG 7 cut(s) 1241, 1313, 1652, 1760, 1937, 2072, 2253
MaeII ACGT 4 cut(s) 38, 356, 1816, 2238
MaeIII GTNAC 6 cut(s) 475, 883, 1381, 1429, 1746, 1994
MboII GAAGA 9 cut(s) 318, 413, 424, 523, 634, 959, 1022, 1331, 2399
MflI RGATCY 3 cut(s) 1012, 1872, 2284
MhlI GDGCHC 4 cut(s) 31, 390, 998, 1353
MlsI TGGCCA 1 cut(s) 1621
MluNI TGGCCA 1 cut(s) 1621
MlyI GAGTC 2 cut(s) 839, 1483
MmeI TCCRAC 1 cut(s) 121
Mox20I TGGCCA 1 cut(s) 1621
MroI TCCGGA 1 cut(s) 1252
MroXI GAANNNNTTC 2 cut(s) 710, 1323
MscI TGGCCA 1 cut(s) 1621
MslI CAYNNNNRTG 3 cut(s) 1289, 2363, 2375
Msp20I TGGCCA 1 cut(s) 1621
MspCI CTTAAG 3 cut(s) 524, 1796, 2009
MspI CCGG 1 cut(s) 1253
MspR9I CCNGG 2 cut(s) 806, 2127
MvaI CCWGG 2 cut(s) 806, 2127
MvnI CGCG 1 cut(s) 1086
NlaIV GGNNCC 5 cut(s) 459, 933, 1603, 2141, 2340
NmuCI GTSAC 4 cut(s) 883, 1381, 1429, 1994
NsbI TGCGCA 1 cut(s) 2369
NspI RCATGY 3 cut(s) 1447, 1630, 2368
NspV TTCGAA 1 cut(s) 729
PaeI GCATGC 1 cut(s) 1447
PdmI GAANNNNTTC 2 cut(s) 710, 1323
PfeI GAWTC 3 cut(s) 776, 909, 1151
PflMI CCANNNNNTGG 1 cut(s) 1969
PfoI TCCNGGA 1 cut(s) 804
PleI GAGTC 2 cut(s) 838, 1482
PpsI GAGTC 2 cut(s) 838, 1482
PpuMI RGGWCCY 1 cut(s) 1843
Psp124BI GAGCTC 2 cut(s) 998, 1353
Psp5II RGGWCCY 1 cut(s) 1843
Psp6I CCWGG 2 cut(s) 804, 2125
PspFI CCCAGC 1 cut(s) 1574
PspGI CCWGG 2 cut(s) 804, 2125
PspN4I GGNNCC 5 cut(s) 459, 933, 1603, 2141, 2340
PspPI GGNCC 3 cut(s) 676, 1258, 1843
PspPPI RGGWCCY 1 cut(s) 1843
PstI CTGCAG 1 cut(s) 2059
PsuI RGATCY 3 cut(s) 1012, 1872, 2284
RsaI GTAC 2 cut(s) 687, 1635
RsaNI GTAC 2 cut(s) 686, 1634
RseI CAYNNNNRTG 3 cut(s) 1289, 2363, 2375
SacI GAGCTC 2 cut(s) 998, 1353
Sau96I GGNCC 3 cut(s) 676, 1258, 1843
SchI GAGTC 2 cut(s) 839, 1483
ScrFI CCNGG 2 cut(s) 806, 2127
SduI GDGCHC 4 cut(s) 31, 390, 998, 1353
SfaNI GCATC 9 cut(s) 49, 334, 560, 940, 986, 1037, 1183, 1912, 2031
SfcI CTRYAG 2 cut(s) 1095, 2055
SfuI TTCGAA 1 cut(s) 729
SinI GGWCC 3 cut(s) 676, 1258, 1843
SmiMI CAYNNNNRTG 3 cut(s) 1289, 2363, 2375
SmlI CTYRAG 5 cut(s) 524, 839, 1796, 2009, 2288
SmoI CTYRAG 5 cut(s) 524, 839, 1796, 2009, 2288
SphI GCATGC 1 cut(s) 1447
SsiI CCGC 4 cut(s) 455, 573, 1086, 1298
SspMI CTAG 7 cut(s) 1241, 1313, 1652, 1760, 1937, 2072, 2253
SstI GAGCTC 2 cut(s) 998, 1353
StyD4I CCNGG 2 cut(s) 804, 2125
StyI CCWWGG 4 cut(s) 174, 585, 1197, 1900
TaaI ACNGT 7 cut(s) 385, 481, 498, 798, 1742, 1854, 2033
TaiI ACGT 4 cut(s) 41, 359, 1819, 2241
TaqI TCGA 9 cut(s) 159, 394, 659, 714, 729, 1019, 1236, 2132, 2163
TatI WGTACW 1 cut(s) 685
TauI GCSGC 1 cut(s) 1089
TfiI GAWTC 3 cut(s) 776, 909, 1151
TscAI CASTG 4 cut(s) 78, 132, 1932, 2252
TseFI GTSAC 4 cut(s) 883, 1381, 1429, 1994
TseI GCWGC 9 cut(s) 237, 560, 718, 746, 1185, 1271, 1274, 2220, 2266
Tsp45I GTSAC 4 cut(s) 883, 1381, 1429, 1994
TspDTI ATGAA 9 cut(s) 431, 530, 546, 705, 960, 1023, 1128, 1332, 1873
TspGWI ACGGA 1 cut(s) 718
TspRI CASTG 4 cut(s) 78, 132, 1932, 2252
Van91I CCANNNNNTGG 1 cut(s) 1969
Vha464I CTTAAG 3 cut(s) 524, 1796, 2009
VpaK11BI GGWCC 3 cut(s) 676, 1258, 1843
XapI RAATTY 4 cut(s) 1323, 1660, 1915, 2156
XbaI TCTAGA 2 cut(s) 1312, 2252
XceI RCATGY 3 cut(s) 1447, 1630, 2368
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmnI GAANNNNTTC 2 cut(s) 710, 1323
XspI CTAG 7 cut(s) 1241, 1313, 1652, 1760, 1937, 2072, 2253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.