Rh2BG177700

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000039
Physical Location & Seq
Forward (+)
15778028 .. 15788067
10040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG177700.1

Sequence Viewer

Length: 2670 bp
ATGGAGCTTGGGGCGGTTCTTCCTTCTTTGGTGATAATCTGCGGAGCTGGTTTAGCGGTGACATTTTGGACGCTGTGTGATTCTGTTAAGCAAGTGAAGGACCAGATACTGATGTTCAAACCCCAACTCGGGGATATCAAGTCCACTCTGGACTCTTTGCAGCCACTGATCAAGGAGATGGCAGAGTGTGAGAAGGAACTGGATGGTCCAGAGGAGGAACTAGAGAATCTTAGAGTGGAAATGCAGGAAGGAGCGGAGCTCATTCGCAAGTGCTCGGAAGCGAGCAGGTTCAAGAAGTATAAGTATGGCAGCAGACTTGTTGAATTGAGCAAGTCACTTCAGAGGCTGTTGGGGATATTGAAAGTGCAGGGCATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGGAACGTTGAGAATGTGGTGCACAGGATTGAAGGGAATTGTGTGATGCAGAACAATCAGTCTGAAAAAATTGAAGGGTGGTGTGCTGTGCCTGAGCCTCCAACAATTACGGTTGGATTGGATTTGCCTTTGAGGGAATTGAAGATGAAGCTCTTTAAGGACGGAGTGTCGATGGTGATACTTACTGCTCCAGGAGGATGCGGGAAAACTACATTGGCAACAAAGTTATGTCAAGATGCAGAAGTCAAAGATAAATTCAAAAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACTTGTACCTAATTGTAAAAGAGCTATATCATCGAACGGGTGCCCAGGTCCCCGATTTCCAAAGTGAAGTAACTGCAGTTAGCTGGCTGCAGAAATTTCTGAAGCAAACAGGTCAAAATCCTATGTTGTTGGTCCTGGATGATGTTTGGGCTGGATCAGAATTCATTCTTGAGAAGTTCGATGAATTAAAAATGACAAATTACAAGATATTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTTTTTGGAATGTTTGAACCGCAAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGTGATAGGAGCTCTTATATTTCTGAAGATCTTCCGAGAAAGATAGTAGAACGTTGTATGGGATATCCACTTGCAATTACAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTGTTGGAATGGTCTAGAGGTTCTTCTGTTCTTAAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGAAGCTTAGATGCTTTGTTTGAAGAGAAGTCTCTTATCAGGGAATGCTTCATAGACCTTGGTTTATTTCCTGAAGATAAAAGAATCTCAGCTACTGTCCTCATTGATATGTGGGCAGAGGTATATGGCATAAATGAGGACATTTCGTCCATTGTGAACCTCTATGAACTTGCCTCTCGGAGTTTGGCTAATCTGGTAGTCACAAGGAAGGACAACAAGGATAGTGATGGCTATTACACTGAACACTTTGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCTGCAGATGAGTCAGGAACCACTGCAACATAGAATGAGGCTCATGATTGACATATGTGGAGACAACCTTCCTATGTGGTGGAAAGAACAAAACTATGAATCCCTCATTGCTCGCCTTGTATCTATCTCGACAGATGAAGAGTTCTCATCTAAATGGCAAAACTTGCAACTACCAGAGGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTGGAGAAAATGAATAAATTGAAGACTCTAGTACTCACAAGTTATGGTATCACTCCGGCTGAGTTGAATAACTTTCAACTAGTTGATTCAAAACCGAATCTGAAGAGAATCAGATTAGAACGAATTTCAATTCCTTCCATAAGCAGGTATCCTATACAGTTGAAAAGTTTGCAAAAGATTTCTCTATTCATGTGTAGAATTGGCCAAGGTTTTGGTAATGGTTCCATCCATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGTTGCCTCCTCAATTGTGTGACCTTGTTCAATTGAAGAAACTCAGTGTCACCAATTCTCATAAACTCTCTGCCTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGCTGTTGAGGCTAAGGTGTTGTACAGACTTGTCAGAGTTACCTGGCTCGGTCAGGAACCTCAAGAAGTTAAACTTGCTTGACATATCTGATTGCTTTAGCATTAAGGAATTGCCTGAAGACATTGGAGAATTGTGCAATTTAGAAAGGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCTTCAGTTTTGGATCTTGAACAGTTGAAGGAAGTGACATGTGATGAAGAGACTGAAATTTTATGGGAACCCTTTTTACCCTTTCTCAAGAACATACACATGAAGGTTGTCAGAGGGCTGGTTTTCAAGGCACATTACAACAGAAACTCGATAGAGCAGCGTCGTCTCCAGATTCTAAAAGATATGCAAGTAACGGCATCTCTGTGGATGTTGAATACATCAATTTGCATTGATCTTAAGTGGATCTCTGGGCAAACGAAGAAACAGTTTTTTTTTAGGAGATTGAGAGATTTGGGTTATCACTTCGGCTCTGGTCAGGCATCAAATCCCCTTTGCATCTGGATTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

889

Amino Acids

101.7

Weight (kDa)

6.26

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 12 - 139 6.5e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 179 - 311 1.5e-15 NB-ARC domain
AAA_22 PF13401 192 - 294 1.9e-06 AAA domain
LRR_14 PF23598 661 - 740 8.5e-09 Leucine-rich repeat region
LRR_14 PF23598 744 - 793 9.7e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 925
Acc36I ACCTGC 2 cut(s) 276, 1866
AccB1I GGYRCC 1 cut(s) 743
AccB7I CCANNNNNTGG 1 cut(s) 944
AccBSI CCGCTC 1 cut(s) 254
AciI CCGC 7 cut(s) 14, 42, 56, 254, 606, 976, 1119
AclI AACGTT 2 cut(s) 411, 1069
AclWI GGATC 3 cut(s) 865, 2340, 2570
AcoI YGGCCR 1 cut(s) 1933
AcsI RAATTY 6 cut(s) 659, 797, 863, 1854, 2125, 2376
AcuI CTGAAG 8 cut(s) 323, 824, 1062, 1308, 1853, 2124, 2268, 2307
AfaI GTAC 3 cut(s) 710, 1764, 2155
AfiI CCNNNNNNNGG 5 cut(s) 128, 129, 130, 944, 1875
AflII CTTAAG 2 cut(s) 1181, 2555
AflIII ACRYGT 1 cut(s) 2357
AhdI GACNNNNNGTC 2 cut(s) 571, 1360
AhlI ACTAGT 1 cut(s) 1810
AjnI CCWGG 4 cut(s) 595, 747, 837, 2173
AjuI GAANNNNNNNTTGG 6 cut(s) 377, 409, 602, 634, 931, 963
Alw21I GWGCWC 5 cut(s) 261, 275, 429, 1031, 2300
Alw26I GTCTC 5 cut(s) 1251, 1554, 2284, 2363, 2489
Alw44I GTGCAC 1 cut(s) 425
AlwI GGATC 3 cut(s) 865, 2340, 2570
AlwNI CAGNNNCTG 4 cut(s) 109, 166, 346, 1310
Ama87I CYCGRG 2 cut(s) 128, 2299
AoxI GGCC 1 cut(s) 1933
ApaLI GTGCAC 1 cut(s) 425
ApeKI GCWGC 6 cut(s) 160, 309, 790, 1007, 1123, 2476
ApoI RAATTY 6 cut(s) 659, 797, 863, 1854, 2125, 2376
ArsI GACNNNNNNTTYG 2 cut(s) 657, 689
Asp700I GAANNNNTTC 2 cut(s) 867, 1047
AspS9I GGNCC 4 cut(s) 100, 206, 751, 835
AsuHPI GGTGA 8 cut(s) 43, 70, 415, 592, 928, 992, 1031, 2065
AvaI CYCGRG 2 cut(s) 128, 2299
AvaII GGWCC 4 cut(s) 100, 206, 751, 835
AxyI CCTNAGG 1 cut(s) 1015
BaeGI GKGCMC 2 cut(s) 429, 748
BaeI ACNNNNGTAYC 2 cut(s) 98, 131
BalI TGGCCA 1 cut(s) 1935
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 2 cut(s) 261, 1031
BbsI GAAGAC 4 cut(s) 667, 1760, 2112, 2256
Bbv12I GWGCWC 5 cut(s) 261, 275, 429, 1031, 2300
BbvI GCAGC 6 cut(s) 172, 321, 777, 1019, 1135, 2488
BccI CCATC 5 cut(s) 172, 197, 571, 1436, 1964
BceAI ACGGC 1 cut(s) 2529
BcgI CGANNNNNNTGC 2 cut(s) 381, 415
BciT130I CCWGG 4 cut(s) 597, 749, 839, 2175
BciVI GTATCC 1 cut(s) 1890
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 5 cut(s) 1251, 1554, 2284, 2363, 2489
BcuI ACTAGT 1 cut(s) 1810
BfaI CTAG 5 cut(s) 221, 1164, 1760, 1811, 2120
BfmI CTRYAG 3 cut(s) 777, 791, 1502
BfrI CTTAAG 2 cut(s) 1181, 2555
BfuAI ACCTGC 2 cut(s) 276, 1866
BfuI GTATCC 1 cut(s) 1890
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 6 cut(s) 161, 310, 791, 1008, 1124, 2477
BlsI GCNGC 6 cut(s) 162, 311, 792, 1009, 1125, 2478
BmcAI AGTACT 1 cut(s) 1764
Bme1390I CCNGG 4 cut(s) 597, 749, 839, 2175
Bme18I GGWCC 4 cut(s) 100, 206, 751, 835
BmeRI GACNNNNNGTC 2 cut(s) 571, 1360
BmeT110I CYCGRG 2 cut(s) 128, 2299
BmgT120I GGNCC 4 cut(s) 100, 206, 751, 835
BmiI GGNNCC 6 cut(s) 745, 753, 1518, 1954, 2189, 2388
BmrFI CCNGG 4 cut(s) 597, 749, 839, 2175
BmsI GCATC 9 cut(s) 441, 593, 631, 973, 1019, 1216, 2525, 2648, 2664
BpiI GAAGAC 4 cut(s) 667, 1760, 2112, 2256
BplI GAGNNNNNCTC 2 cut(s) 243, 275
BpmI CTGGAG 2 cut(s) 579, 2471
Bpu10I CCTNAGC 3 cut(s) 498, 1484, 2144
BpuEI CTTGAG 3 cut(s) 893, 2177, 2390
BsaJI CCNNGG 3 cut(s) 747, 1273, 1936
Bsc4I CCNNNNNNNGG 5 cut(s) 128, 129, 130, 944, 1875
Bse1I ACTGG 1 cut(s) 204
Bse21I CCTNAGG 1 cut(s) 1015
Bse3DI GCAATG 4 cut(s) 676, 993, 1605, 2017
BseBI CCWGG 4 cut(s) 597, 749, 839, 2175
BseDI CCNNGG 3 cut(s) 747, 1273, 1936
BseGI GGATG 7 cut(s) 208, 385, 608, 847, 1010, 1956, 2532
BseLI CCNNNNNNNGG 5 cut(s) 128, 129, 130, 944, 1875
BseMI GCAATG 4 cut(s) 676, 993, 1605, 2017
BseMII CTCAG 5 cut(s) 489, 1317, 1716, 1782, 2080
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 2 cut(s) 227, 2022
BseSI GKGCMC 2 cut(s) 429, 748
BseXI GCAGC 6 cut(s) 172, 321, 777, 1019, 1135, 2488
BsgI GTGCAG 1 cut(s) 386
BshFI GGCC 1 cut(s) 1935
BshNI GGYRCC 1 cut(s) 743
BsiHKAI GWGCWC 5 cut(s) 261, 275, 429, 1031, 2300
BsiHKCI CYCGRG 2 cut(s) 128, 2299
BsiSI CCGG 1 cut(s) 1787
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 5 cut(s) 128, 129, 130, 944, 1875
BsmAI GTCTC 5 cut(s) 1251, 1554, 2284, 2363, 2489
BsmBI CGTCTC 1 cut(s) 2489
BsmFI GGGAC 1 cut(s) 737
BsmI GAATGC 1 cut(s) 1265
BsnI GGCC 1 cut(s) 1935
BsoBI CYCGRG 2 cut(s) 128, 2299
Bsp1286I GDGCHC 6 cut(s) 261, 275, 429, 748, 1031, 2300
Bsp1407I TGTACA 1 cut(s) 2153
Bsp143I GATC 7 cut(s) 168, 857, 1045, 1109, 2332, 2551, 2562
BspACI CCGC 7 cut(s) 14, 42, 56, 254, 606, 976, 1119
BspANI GGCC 1 cut(s) 1935
BspCNI CTCAG 5 cut(s) 490, 1316, 1717, 1783, 2079
BspHI TCATGA 1 cut(s) 1542
BspLI GGNNCC 6 cut(s) 745, 753, 1518, 1954, 2189, 2388
BspMAI CTGCAG 3 cut(s) 781, 795, 1506
BspMI ACCTGC 2 cut(s) 276, 1866
BspPI GGATC 3 cut(s) 865, 2340, 2570
BspT107I GGYRCC 1 cut(s) 743
BspTI CTTAAG 2 cut(s) 1181, 2555
BsrBI CCGCTC 1 cut(s) 254
BsrDI GCAATG 4 cut(s) 676, 993, 1605, 2017
BsrGI TGTACA 1 cut(s) 2153
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 3 cut(s) 747, 1273, 1936
BssMI GATC 7 cut(s) 168, 857, 1045, 1109, 2332, 2551, 2562
BssT1I CCWWGG 2 cut(s) 1273, 1936
Bst2UI CCWGG 4 cut(s) 597, 749, 839, 2175
Bst4CI ACNGT 8 cut(s) 517, 688, 1099, 1312, 1890, 2295, 2343, 2586
Bst6I CTCTTC 4 cut(s) 1233, 1632, 1829, 2361
BstAFI CTTAAG 2 cut(s) 1181, 2555
BstAPI GCANNNNNTGC 2 cut(s) 1480, 1663
BstAUI TGTACA 1 cut(s) 2153
BstC8I GCNNGC 4 cut(s) 283, 788, 1121, 1612
BstF5I GGATG 7 cut(s) 208, 385, 608, 847, 1010, 1956, 2532
BstKTI GATC 7 cut(s) 171, 860, 1048, 1112, 2335, 2554, 2565
BstMAI GTCTC 5 cut(s) 1251, 1554, 2284, 2363, 2489
BstMBI GATC 7 cut(s) 168, 857, 1045, 1109, 2332, 2551, 2562
BstMWI GCNNNNNNNGC 4 cut(s) 53, 1480, 1663, 2140
BstNI CCWGG 4 cut(s) 597, 749, 839, 2175
BstNSI RCATGY 2 cut(s) 1483, 2361
BstSCI CCNGG 4 cut(s) 595, 747, 837, 2173
BstSFI CTRYAG 3 cut(s) 777, 791, 1502
BstSLI GKGCMC 2 cut(s) 429, 748
BstV1I GCAGC 6 cut(s) 172, 321, 777, 1019, 1135, 2488
BstV2I GAAGAC 4 cut(s) 667, 1760, 2112, 2256
BstX2I RGATCY 3 cut(s) 1045, 2332, 2562
BstXI CCANNNNNNTGG 1 cut(s) 1943
BstYI RGATCY 3 cut(s) 1045, 2332, 2562
Bsu36I CCTNAGG 1 cut(s) 1015
BsuI GTATCC 1 cut(s) 1890
BsuRI GGCC 1 cut(s) 1935
BtsCI GGATG 7 cut(s) 208, 385, 608, 847, 1010, 1956, 2532
BtsI GCAGTG 1 cut(s) 1520
BtsIMutI CAGTG 5 cut(s) 164, 1452, 1520, 2074, 2300
BveI ACCTGC 2 cut(s) 276, 1866
Cac8I GCNNGC 4 cut(s) 283, 788, 1121, 1612
CaiI CAGNNNCTG 4 cut(s) 109, 166, 346, 1310
CciI TCATGA 1 cut(s) 1542
Cfr13I GGNCC 4 cut(s) 100, 206, 751, 835
CseI GACGC 2 cut(s) 79, 2468
Csp6I GTAC 3 cut(s) 709, 1763, 2154
CviAII CATG 9 cut(s) 1004, 1193, 1475, 1480, 1543, 1921, 2287, 2358, 2419
CviQI GTAC 3 cut(s) 709, 1763, 2154
DpnI GATC 7 cut(s) 170, 859, 1047, 1111, 2334, 2553, 2564
DpnII GATC 7 cut(s) 168, 857, 1045, 1109, 2332, 2551, 2562
DrdI GACNNNNNNGTC 1 cut(s) 925
DriI GACNNNNNGTC 2 cut(s) 571, 1360
DseDI GACNNNNNNGTC 1 cut(s) 925
EaeI YGGCCR 1 cut(s) 1933
Eam1104I CTCTTC 4 cut(s) 1233, 1632, 1829, 2361
Eam1105I GACNNNNNGTC 2 cut(s) 571, 1360
EarI CTCTTC 4 cut(s) 1233, 1632, 1829, 2361
Ecl136II GAGCTC 2 cut(s) 259, 1029
Eco130I CCWWGG 2 cut(s) 1273, 1936
Eco24I GRGCYC 2 cut(s) 261, 1031
Eco32I GATATC 2 cut(s) 136, 1082
Eco47I GGWCC 4 cut(s) 100, 206, 751, 835
Eco53kI GAGCTC 2 cut(s) 259, 1029
Eco57I CTGAAG 8 cut(s) 323, 824, 1062, 1308, 1853, 2124, 2268, 2307
Eco81I CCTNAGG 1 cut(s) 1015
Eco88I CYCGRG 2 cut(s) 128, 2299
EcoICRI GAGCTC 2 cut(s) 259, 1029
EcoO109I RGGNCCY 1 cut(s) 751
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 4 cut(s) 595, 747, 837, 2173
EcoRV GATATC 2 cut(s) 136, 1082
EcoT14I CCWWGG 2 cut(s) 1273, 1936
EcoT38I GRGCYC 2 cut(s) 261, 1031
ErhI CCWWGG 2 cut(s) 1273, 1936
Esp3I CGTCTC 1 cut(s) 2489
FaeI CATG 9 cut(s) 1007, 1196, 1478, 1483, 1546, 1924, 2290, 2361, 2422
FalI AAGNNNNNCTT 6 cut(s) 689, 721, 916, 948, 2189, 2221
FaqI GGGAC 1 cut(s) 737
FatI CATG 9 cut(s) 1003, 1192, 1474, 1479, 1542, 1920, 2286, 2357, 2418
FauI CCCGC 2 cut(s) 599, 1112
FauNDI CATATG 1 cut(s) 1553
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 6 cut(s) 161, 310, 791, 1008, 1124, 2477
FokI GGATG 7 cut(s) 215, 392, 615, 854, 997, 1943, 2539
FriOI GRGCYC 2 cut(s) 261, 1031
Fsp4HI GCNGC 6 cut(s) 161, 310, 791, 1008, 1124, 2477
FspBI CTAG 5 cut(s) 221, 1164, 1760, 1811, 2120
GluI GCNGC 6 cut(s) 161, 310, 791, 1008, 1124, 2477
GsuI CTGGAG 2 cut(s) 579, 2471
HaeIII GGCC 1 cut(s) 1935
HapII CCGG 1 cut(s) 1787
HgaI GACGC 2 cut(s) 79, 2468
Hin1II CATG 9 cut(s) 1007, 1196, 1478, 1483, 1546, 1924, 2290, 2361, 2422
HindIII AAGCTT 1 cut(s) 1219
HpaII CCGG 1 cut(s) 1787
HphI GGTGA 8 cut(s) 43, 70, 415, 592, 928, 992, 1031, 2065
Hpy166II GTNNAC 3 cut(s) 144, 427, 1372
Hpy8I GTNNAC 3 cut(s) 144, 427, 1372
Hpy99I CGWCG 1 cut(s) 2484
HpyCH4III ACNGT 8 cut(s) 517, 688, 1099, 1312, 1890, 2295, 2343, 2586
HpyCH4IV ACGT 2 cut(s) 411, 1069
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 1480, 1663, 2140
HpySE526I ACGT 2 cut(s) 411, 1069
Hsp92II CATG 9 cut(s) 1007, 1196, 1478, 1483, 1546, 1924, 2290, 2361, 2422
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 7 cut(s) 168, 857, 1045, 1109, 2332, 2551, 2562
LmnI GCTCC 6 cut(s) 4, 44, 251, 256, 598, 1026
Lsp1109I GCAGC 6 cut(s) 172, 321, 777, 1019, 1135, 2488
LweI GCATC 9 cut(s) 441, 593, 631, 973, 1019, 1216, 2525, 2648, 2664
MaeI CTAG 5 cut(s) 221, 1164, 1760, 1811, 2120
MaeII ACGT 2 cut(s) 411, 1069
MalI GATC 7 cut(s) 170, 859, 1047, 1111, 2334, 2553, 2564
MbiI CCGCTC 1 cut(s) 254
MboI GATC 7 cut(s) 168, 857, 1045, 1109, 2332, 2551, 2562
MfeI CAATTG 2 cut(s) 2036, 2054
MflI RGATCY 3 cut(s) 1045, 2332, 2562
MhlI GDGCHC 6 cut(s) 261, 275, 429, 748, 1031, 2300
MlsI TGGCCA 1 cut(s) 1935
MluNI TGGCCA 1 cut(s) 1935
MlyI GAGTC 3 cut(s) 146, 1519, 1750
MmeI TCCRAC 4 cut(s) 499, 530, 1084, 1134
Mox20I TGGCCA 1 cut(s) 1935
MroXI GAANNNNTTC 2 cut(s) 867, 1047
MscI TGGCCA 1 cut(s) 1935
MseI TTAA 7 cut(s) 87, 561, 890, 1182, 2201, 2235, 2556
MslI CAYNNNNRTG 4 cut(s) 1322, 1651, 2417, 2521
Msp20I TGGCCA 1 cut(s) 1935
MspCI CTTAAG 2 cut(s) 1181, 2555
MspI CCGG 1 cut(s) 1787
MspR9I CCNGG 4 cut(s) 597, 749, 839, 2175
MunI CAATTG 2 cut(s) 2036, 2054
Mva1269I GAATGC 1 cut(s) 1265
MvaI CCWGG 4 cut(s) 597, 749, 839, 2175
MwoI GCNNNNNNNGC 4 cut(s) 53, 1480, 1663, 2140
NdeI CATATG 1 cut(s) 1553
NdeII GATC 7 cut(s) 168, 857, 1045, 1109, 2332, 2551, 2562
NlaIII CATG 9 cut(s) 1007, 1196, 1478, 1483, 1546, 1924, 2290, 2361, 2422
NlaIV GGNNCC 6 cut(s) 745, 753, 1518, 1954, 2189, 2388
NmuCI GTSAC 7 cut(s) 58, 333, 916, 1414, 2042, 2071, 2353
NspI RCATGY 2 cut(s) 1483, 2361
PaeR7I CTCGAG 1 cut(s) 2299
PagI TCATGA 1 cut(s) 1542
PciI ACATGT 1 cut(s) 2357
PctI GAATGC 1 cut(s) 1265
PdmI GAANNNNTTC 2 cut(s) 867, 1047
PfeI GAWTC 9 cut(s) 80, 226, 1299, 1598, 1817, 1828, 1839, 2491, 2662
PflMI CCANNNNNTGG 1 cut(s) 944
PfoI TCCNGGA 2 cut(s) 595, 837
PkrI GCNGC 6 cut(s) 162, 311, 792, 1009, 1125, 2478
PleI GAGTC 3 cut(s) 146, 1518, 1750
PpsI GAGTC 3 cut(s) 146, 1518, 1750
PpuMI RGGWCCY 1 cut(s) 751
PscI ACATGT 1 cut(s) 2357
Psp124BI GAGCTC 2 cut(s) 261, 1031
Psp1406I AACGTT 2 cut(s) 411, 1069
Psp5II RGGWCCY 1 cut(s) 751
Psp6I CCWGG 4 cut(s) 595, 747, 837, 2173
PspGI CCWGG 4 cut(s) 595, 747, 837, 2173
PspN4I GGNNCC 6 cut(s) 745, 753, 1518, 1954, 2189, 2388
PspPI GGNCC 4 cut(s) 100, 206, 751, 835
PspPPI RGGWCCY 1 cut(s) 751
PsrI GAACNNNNNNTAC 2 cut(s) 2379, 2411
PstI CTGCAG 3 cut(s) 781, 795, 1506
PstNI CAGNNNCTG 4 cut(s) 109, 166, 346, 1310
PsuI RGATCY 3 cut(s) 1045, 2332, 2562
RsaI GTAC 3 cut(s) 710, 1764, 2155
RsaNI GTAC 3 cut(s) 709, 1763, 2154
RseI CAYNNNNRTG 4 cut(s) 1322, 1651, 2417, 2521
SacI GAGCTC 2 cut(s) 261, 1031
SaqAI TTAA 7 cut(s) 87, 561, 890, 1182, 2201, 2235, 2556
SatI GCNGC 6 cut(s) 161, 310, 791, 1008, 1124, 2477
Sau3AI GATC 7 cut(s) 168, 857, 1045, 1109, 2332, 2551, 2562
Sau96I GGNCC 4 cut(s) 100, 206, 751, 835
ScaI AGTACT 1 cut(s) 1764
SchI GAGTC 3 cut(s) 146, 1519, 1750
ScrFI CCNGG 4 cut(s) 597, 749, 839, 2175
SduI GDGCHC 6 cut(s) 261, 275, 429, 748, 1031, 2300
SfaNI GCATC 9 cut(s) 441, 593, 631, 973, 1019, 1216, 2525, 2648, 2664
SfcI CTRYAG 3 cut(s) 777, 791, 1502
Sfr274I CTCGAG 1 cut(s) 2299
SinI GGWCC 4 cut(s) 100, 206, 751, 835
SlaI CTCGAG 1 cut(s) 2299
SmiMI CAYNNNNRTG 4 cut(s) 1322, 1651, 2417, 2521
SmlI CTYRAG 6 cut(s) 872, 1181, 2192, 2299, 2405, 2555
SmoI CTYRAG 6 cut(s) 872, 1181, 2192, 2299, 2405, 2555
SpeI ACTAGT 1 cut(s) 1810
SsiI CCGC 7 cut(s) 14, 42, 56, 254, 606, 976, 1119
SspI AATATT 1 cut(s) 1999
SspMI CTAG 5 cut(s) 221, 1164, 1760, 1811, 2120
SstI GAGCTC 2 cut(s) 261, 1031
StyD4I CCNGG 4 cut(s) 595, 747, 837, 2173
StyI CCWWGG 2 cut(s) 1273, 1936
TaaI ACNGT 8 cut(s) 517, 688, 1099, 1312, 1890, 2295, 2343, 2586
TaiI ACGT 2 cut(s) 414, 1072
TaqI TCGA 6 cut(s) 575, 736, 882, 1628, 2300, 2468
TaqII GACCGA 1 cut(s) 2170
TatI WGTACW 2 cut(s) 1762, 2153
TfiI GAWTC 9 cut(s) 80, 226, 1299, 1598, 1817, 1828, 1839, 2491, 2662
Tru1I TTAA 7 cut(s) 87, 561, 890, 1182, 2201, 2235, 2556
Tru9I TTAA 7 cut(s) 87, 561, 890, 1182, 2201, 2235, 2556
TscAI CASTG 5 cut(s) 171, 1459, 1527, 2074, 2300
TseFI GTSAC 7 cut(s) 58, 333, 916, 1414, 2042, 2071, 2353
TseI GCWGC 6 cut(s) 160, 309, 790, 1007, 1123, 2476
Tsp45I GTSAC 7 cut(s) 58, 333, 916, 1414, 2042, 2071, 2353
TspGWI ACGGA 1 cut(s) 582
TspRI CASTG 5 cut(s) 171, 1459, 1527, 2074, 2300
Van91I CCANNNNNTGG 1 cut(s) 944
Vha464I CTTAAG 2 cut(s) 1181, 2555
VneI GTGCAC 1 cut(s) 425
VpaK11BI GGWCC 4 cut(s) 100, 206, 751, 835
XapI RAATTY 6 cut(s) 659, 797, 863, 1854, 2125, 2376
XbaI TCTAGA 1 cut(s) 1163
XceI RCATGY 2 cut(s) 1483, 2361
XcmI CCANNNNNNNNNTGG 1 cut(s) 1134
XhoI CTCGAG 1 cut(s) 2299
XmnI GAANNNNTTC 2 cut(s) 867, 1047
XspI CTAG 5 cut(s) 221, 1164, 1760, 1811, 2120
ZrmI AGTACT 1 cut(s) 1764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.