FvH4_6g50140

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000039
Physical Location & Seq
Reverse (-)
37367720 .. 37372051
4332 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50140.t1

Sequence Viewer

Length: 2868 bp
ATGGCAATGGCTGTGGATTTCGTTGTGGGAAAAGCATTCGAGGCACTCTATGCGGGCGTTGAAGAGGTCATTCGGAATAATCTGATATTCTCCGATCTCTGCAAGGACATCAAAGACAGGCTCGACTGTTTAGCACCACTGATCCACGAGATGGGGAAATATAACCAACAATTGGATCTTACAAAGGAGTTGGAAGCTTTAGAAGGACTCATAAAGCGGGGGACAGAGCTCGTCAGCAAATGCTCTAAGCTTGGTAAGTGGAGCGTCCGGAACATCTACAAGAAGCAGAAGTACTCGAAGGAATTTCTTGAATTCAAAGAGTCTCTCCAAAAGGAGTTAGAGGTACTCAAGGTGCAGGTGGCAAGAGATGTGAAGAAGACTGGAGTTGCCGTGGAGAGTATGGCAATAGAAGTGAAGAAGACTGGAGTTGCCGCGGAGAATATCCAGGTTGTGCTTCAAAACATAGAAAGGGGTGTTTCATTGCTTTTGAAGAAGAATGAGGATTTAGAAATTGAAGCTTTAAGTGCAGCACTGCCTGAACTCCCAGAGCTGATAGTCGGATTGGAAGTTCCTTTGGAGCAACTGAAGGGGAAGATTCTTAAGGATGAGAGATCCATGCTCACCCTGACTGGTCCTGCAGGATGCGGCAAGACTACTTTGGCAGCAAAGTTTTGTCAAGATCAAGACATCAAAGATAAATTCCAGGAAAACATCTTCTTCGTCACTTTTACTAAAAAGCCCAACTTGGAGCGTATTATGAAAGGGTTATATGAACAAAAGGGTTATAAAGGACCTGCTTCCCATAATGAAGCAACTCCAGTGAAATGGCTTGAAAGGGTTGAGATGGAAAAAGGTGAAGATCCTTTATTGTTGGTCTTGGATGATGTTTGGTCTGGATCAAAATCTCTTCTTGAGAAGTTTGAGTACAACATGTCAAATTACAAGATTTTAGTGACATCGAGGTCTGAATTTCCGAAATTTGGTCCTTCATATCATCTACAACTATTAGATTATGAAAATGCAATGCAAGTATTTCATCATACAGCATCCTTGGGAGACAAGAGCTCTCATATTCCAGAACATCTTTCGAAAGAGATAGTAAAATATTGCAATGGGTTTCCATTGACCATTACAGTGGCCGGAAGATCACTTTGCGGGCAGCCTGAAGAAATGTGGCGAAGTAGAGTAGATGATTGGTCGGAAAGTTCTTCCATTCTTGATTTTGAGACGGGTCTGCTTCTTCAGCTCCAAACCAGCATAGATGTCTTAGAAAATGCTCCGCCTAAGGTCAAAAAATGTTTTTTCGACCTATGTGCTTTTCCGGGAGATGTTGAAATCCAAGTTACAGCTCTCTTTGATATGTGGGCAGAGTTGTATGATCTAAAGAAAGAGACGTCATGCATTGGCAAACTCTATGAACTCAACAATCGCGGTCTGGCTCTTTTACAAGAACCAGAAGGGGATAAACGTTTCGGAGCGGTACAGGAGGCAAAAGATTTCAGTGAATACGTCGTTAGACAACATGATTTGCTTCGAAAACTGGCTATCTACAACTTGAAAGGGGATCCGGTGGAGGATAGAAAAAGACTAATAATAGAGATATGTGGAAACAATTTTCCCAAATGGTGGAGAGAACAAAAATATCCATACATCAATGCGCGACTTGTATCTATCTCAACTGATAGAGAGTTCTCAGTGAAACTGCCCAACATGGAATTTCCAGAGGCAGAGGTTCTAATCTTGAATTTTCAATCAGAAACGCTCTTTGCCTTACCCGAATTTGTGGAGAGAATGCATAAACTCAAGATGCTGCTGGTCCTAAATTACTCTGGCTGTTATGTTGAAATGGATAATTTTGAGCTACTAGGCTCCTTATCTAATCTTAGGAGTCTCAGATTAGACGGCATCAATTTTTCTTGCATAACCAAGAGCCCAGTACAGTTGAAGAATCTACACAAGTTGTATTTGAGAGCAGGGAGCTACGAAGTTGAAGAGGAAGATGAATGGAGCTATGACGACGAAGATCCTGTTGAATTGTCACATGTATTTCCAAATATTAACGAAATGGTCATTGAGGCCTTCGATTTCTGGGAATGTCCTGCAGGGCTCTGCGAGCTGGTTCATCTAACAAAGCTCAAAATCATTTCTTCTGGTCGCAGTTCATTTGAGTTGCCTGAAGCCATTGGAAATCTGGTCAGATTAGAAGTTTTGAAGCTTGAGAACTTTGGAGTGCTTCCTGACTCGATTGGAAATCTCAAGAAATTAAAATTGCTCATCATCAGCCGCTTTTATTCGAAAGATGCCTTGCCTGAATCGATTGGAAGTCTGGTCAGTTTAAAAGTGTTGAGGGTTGAGGACTGCAAAAATCTTTCAGCCGTTCCAGACTCGATTTGCAATCTCAAGAAATTAAAATTTCTTAAAATTTCTAACAATAATGCATTTCAGTTTTTGCCTGAACAAATGGGCAAGCTCAAGTGTCTGAGACATCTCATCATCGACCGCTGTGATCGGCTATATAAGTTGCCTGAATCGATTGGAAAATTGATCAATTTAGAAGTATTGAGTCTAAGGTACTGTTACATATTGTCGGAGTTGCCTAAGTCGATTAGAAATCTCCAAAAGTTAAACTTTCTTGACATCGATGCATGCTGCAGAATAGAGGAGTTGCCTGAGCATGTTGGTGAATTGAAGAGTTTAAGAAAGCTGCAGATGGTTTTCTGTAATGTATTGAGAAAACTGCCTGAATCAGTTTCACATCTTGAGCAGTTAGAGGAAGTGATATGCGAAGATTGGCAGGCAAGAGAGTTATGGACACCCTTTTTACCCACTCTCAAAAACCTTAGCATCGTGCTCTGGAAGCCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

956

Amino Acids

109.42

Weight (kDa)

5.69

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 139 2.5e-15 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 191 - 323 1.6e-14 NB-ARC domain
AAA_22 PF13401 203 - 306 1.4e-06 AAA domain
LRR_14 PF23598 703 - 762 3.2e-07 Leucine-rich repeat region
LRR_14 PF23598 767 - 832 4.4e-10 Leucine-rich repeat region
LRR_14 PF23598 820 - 948 5.3e-16 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
AarI CACCTGC 1 cut(s) 346
AasI GACNNNNNNGTC 1 cut(s) 961
AatII GACGTC 1 cut(s) 1397
Acc36I ACCTGC 2 cut(s) 346, 802
AccB7I CCANNNNNTGG 3 cut(s) 151, 172, 1626
AccBSI CCGCTC 1 cut(s) 1478
AccII CGCG 3 cut(s) 434, 1431, 1660
AccIII TCCGGA 1 cut(s) 267
AclI AACGTT 1 cut(s) 1468
AclWI GGATC 8 cut(s) 136, 183, 606, 854, 904, 1559, 1572, 2018
AcoI YGGCCR 1 cut(s) 1137
AcuI CTGAAG 4 cut(s) 605, 1185, 1226, 2196
AcyI GRCGYC 1 cut(s) 1394
AfaI GTAC 6 cut(s) 293, 345, 926, 1482, 1938, 2573
AfiI CCNNNNNNNGG 4 cut(s) 151, 172, 980, 1626
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 2 cut(s) 930, 2041
AjnI CCWGG 2 cut(s) 444, 702
AloI GAACNNNNNNTCC 2 cut(s) 552, 584
Alw21I GWGCWC 3 cut(s) 231, 1067, 2853
Alw26I GTCTC 6 cut(s) 327, 1050, 1220, 1385, 1895, 2476
AlwI GGATC 8 cut(s) 136, 183, 606, 854, 904, 1559, 1572, 2018
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 2 cut(s) 1137, 2076
ApeKI GCWGC 6 cut(s) 527, 662, 1159, 1810, 2649, 2704
AspLEI GCGC 1 cut(s) 1660
AspS9I GGNCC 4 cut(s) 632, 791, 983, 1816
AsuC2I CCSGG 1 cut(s) 1323
AsuHPI GGTGA 3 cut(s) 613, 866, 2693
AsuII TTCGAA 3 cut(s) 1088, 1534, 2294
AvaII GGWCC 4 cut(s) 632, 791, 983, 1816
AxyI CCTNAGG 1 cut(s) 1284
BamHI GGATCC 1 cut(s) 1564
BanII GRGCYC 4 cut(s) 231, 1067, 1934, 2109
BarI GAAGNNNNNNTAC 4 cut(s) 275, 307, 908, 940
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 2 cut(s) 383, 425
Bbv12I GWGCWC 3 cut(s) 231, 1067, 2853
BbvI GCAGC 6 cut(s) 539, 674, 1171, 1797, 2636, 2691
BccI CCATC 3 cut(s) 145, 838, 2704
BceAI ACGGC 3 cut(s) 374, 1918, 2360
BcgI CGANNNNNNTGC 2 cut(s) 1295, 1329
BciT130I CCWGG 2 cut(s) 446, 704
BclI TGATCA 1 cut(s) 2544
BcnI CCSGG 1 cut(s) 1323
BcoDI GTCTC 6 cut(s) 327, 1050, 1220, 1385, 1895, 2476
BfaI CTAG 1 cut(s) 1865
BfmI CTRYAG 4 cut(s) 636, 2100, 2650, 2705
BfrI CTTAAG 1 cut(s) 599
BfuAI ACCTGC 2 cut(s) 346, 802
BisI GCNGC 9 cut(s) 432, 528, 646, 663, 1160, 1811, 2284, 2650, 2705
BlsI GCNGC 9 cut(s) 433, 529, 647, 664, 1161, 1812, 2285, 2651, 2706
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 3 cut(s) 446, 704, 1323
Bme18I GGWCC 4 cut(s) 632, 791, 983, 1816
BmgT120I GGNCC 4 cut(s) 632, 791, 983, 1816
BmiI GGNNCC 2 cut(s) 1566, 1870
BmrFI CCNGG 3 cut(s) 446, 704, 1323
BmrI ACTGGG 1 cut(s) 1928
BmsI GCATC 7 cut(s) 632, 1055, 1797, 1914, 2290, 2632, 2853
BmuI ACTGGG 1 cut(s) 1928
BpiI GAAGAC 2 cut(s) 383, 425
BpmI CTGGAG 3 cut(s) 402, 444, 801
Bpu10I CCTNAGC 2 cut(s) 2670, 2840
Bpu14I TTCGAA 3 cut(s) 1088, 1534, 2294
BpuEI CTTGAG 8 cut(s) 332, 932, 1787, 2237, 2240, 2384, 2456, 2780
BpuMI CCSGG 1 cut(s) 1323
Bsa29I ATCGAT 3 cut(s) 2315, 2531, 2640
BsaBI GATNNNNATC 1 cut(s) 901
BsaHI GRCGYC 1 cut(s) 1394
BsaJI CCNNGG 3 cut(s) 390, 432, 1050
BsaWI WCCGGW 2 cut(s) 267, 1567
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 172, 980, 1626
Bse1I ACTGG 6 cut(s) 385, 427, 634, 818, 1545, 1934
Bse21I CCTNAGG 1 cut(s) 1284
Bse3DI GCAATG 4 cut(s) 12, 479, 1029, 1117
Bse8I GATNNNNATC 1 cut(s) 901
BseAI TCCGGA 1 cut(s) 267
BseBI CCWGG 2 cut(s) 446, 704
BseCI ATCGAT 3 cut(s) 2315, 2531, 2640
BseDI CCNNGG 3 cut(s) 390, 432, 1050
BseGI GGATG 4 cut(s) 610, 647, 886, 1046
BseJI GATNNNNATC 1 cut(s) 901
BseLI CCNNNNNNNGG 4 cut(s) 151, 172, 980, 1626
BseMI GCAATG 4 cut(s) 12, 479, 1029, 1117
BseMII CTCAG 4 cut(s) 1707, 1906, 2471, 2661
BseNI ACTGG 6 cut(s) 385, 427, 634, 818, 1545, 1934
BseRI GAGGAG 1 cut(s) 2675
BseXI GCAGC 6 cut(s) 539, 674, 1171, 1797, 2636, 2691
BsgI GTGCAG 2 cut(s) 374, 546
Bsh1236I CGCG 3 cut(s) 434, 1431, 1660
Bsh1285I CGRYCG 1 cut(s) 2500
BshFI GGCC 2 cut(s) 1139, 2078
BshVI ATCGAT 3 cut(s) 2315, 2531, 2640
BsiEI CGRYCG 1 cut(s) 2500
BsiHKAI GWGCWC 3 cut(s) 231, 1067, 2853
BsiSI CCGG 4 cut(s) 268, 1140, 1322, 1568
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 4 cut(s) 151, 172, 980, 1626
BsmAI GTCTC 6 cut(s) 327, 1050, 1220, 1385, 1895, 2476
BsmBI CGTCTC 2 cut(s) 1220, 1385
BsmFI GGGAC 1 cut(s) 235
BsmI GAATGC 2 cut(s) 35, 1797
BsnI GGCC 2 cut(s) 1139, 2078
Bsp119I TTCGAA 3 cut(s) 1088, 1534, 2294
Bsp1286I GDGCHC 5 cut(s) 231, 1067, 1934, 2109, 2853
Bsp13I TCCGGA 1 cut(s) 267
BspANI GGCC 2 cut(s) 1139, 2078
BspCNI CTCAG 4 cut(s) 1706, 1905, 2472, 2662
BspDI ATCGAT 3 cut(s) 2315, 2531, 2640
BspEI TCCGGA 1 cut(s) 267
BspFNI CGCG 3 cut(s) 434, 1431, 1660
BspLI GGNNCC 2 cut(s) 1566, 1870
BspMAI CTGCAG 4 cut(s) 640, 2104, 2654, 2709
BspMI ACCTGC 2 cut(s) 346, 802
BspPI GGATC 8 cut(s) 136, 183, 606, 854, 904, 1559, 1572, 2018
BspT104I TTCGAA 3 cut(s) 1088, 1534, 2294
BspTI CTTAAG 1 cut(s) 599
BsrBI CCGCTC 1 cut(s) 1478
BsrDI GCAATG 4 cut(s) 12, 479, 1029, 1117
BsrI ACTGG 6 cut(s) 385, 427, 634, 818, 1545, 1934
BssECI CCNNGG 3 cut(s) 390, 432, 1050
BssNI GRCGYC 1 cut(s) 1394
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 1050
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 2 cut(s) 446, 704
Bst4CI ACNGT 4 cut(s) 128, 1135, 1941, 2576
Bst6I CTCTTC 4 cut(s) 57, 912, 1986, 2684
BstACI GRCGYC 1 cut(s) 1394
BstAFI CTTAAG 1 cut(s) 599
BstAPI GCANNNNNTGC 1 cut(s) 50
BstBI TTCGAA 3 cut(s) 1088, 1534, 2294
BstC8I GCNNGC 6 cut(s) 55, 1157, 2114, 2468, 2647, 2796
BstDSI CCRYGG 2 cut(s) 390, 432
BstF5I GGATG 4 cut(s) 610, 647, 886, 1046
BstFNI CGCG 3 cut(s) 434, 1431, 1660
BstHHI GCGC 1 cut(s) 1660
BstMAI GTCTC 6 cut(s) 327, 1050, 1220, 1385, 1895, 2476
BstMCI CGRYCG 1 cut(s) 2500
BstMWI GCNNNNNNNGC 6 cut(s) 41, 50, 524, 1243, 2113, 2857
BstNI CCWGG 2 cut(s) 446, 704
BstNSI RCATGY 4 cut(s) 934, 2045, 2649, 2678
BstSCI CCNGG 3 cut(s) 444, 702, 1321
BstSFI CTRYAG 4 cut(s) 636, 2100, 2650, 2705
BstUI CGCG 3 cut(s) 434, 1431, 1660
BstV1I GCAGC 6 cut(s) 539, 674, 1171, 1797, 2636, 2691
BstV2I GAAGAC 2 cut(s) 383, 425
BstX2I RGATCY 5 cut(s) 175, 611, 859, 1564, 2023
BstXI CCANNNNNNTGG 2 cut(s) 825, 1135
BstYI RGATCY 5 cut(s) 175, 611, 859, 1564, 2023
Bsu15I ATCGAT 3 cut(s) 2315, 2531, 2640
Bsu36I CCTNAGG 1 cut(s) 1284
BsuRI GGCC 2 cut(s) 1139, 2078
BsuTUI ATCGAT 3 cut(s) 2315, 2531, 2640
BtgI CCRYGG 2 cut(s) 390, 432
BtsCI GGATG 4 cut(s) 610, 647, 886, 1046
BtsI GCAGTG 1 cut(s) 530
BtsIMutI CAGTG 6 cut(s) 137, 530, 825, 1140, 1507, 1701
BveI ACCTGC 2 cut(s) 346, 802
Cac8I GCNNGC 6 cut(s) 55, 1157, 2114, 2468, 2647, 2796
CfoI GCGC 1 cut(s) 1660
Cfr13I GGNCC 4 cut(s) 632, 791, 983, 1816
Cfr42I CCGCGG 1 cut(s) 435
ClaI ATCGAT 3 cut(s) 2315, 2531, 2640
CseI GACGC 1 cut(s) 253
Csp6I GTAC 6 cut(s) 292, 344, 925, 1481, 1937, 2572
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 8 cut(s) 616, 931, 1398, 1523, 1711, 2042, 2646, 2675
CviQI GTAC 6 cut(s) 292, 344, 925, 1481, 1937, 2572
DraI TTTAAA 1 cut(s) 2337
DrdI GACNNNNNNGTC 1 cut(s) 961
DseDI GACNNNNNNGTC 1 cut(s) 961
EaeI YGGCCR 1 cut(s) 1137
Eam1104I CTCTTC 4 cut(s) 57, 912, 1986, 2684
EarI CTCTTC 4 cut(s) 57, 912, 1986, 2684
EciI GGCGGA 1 cut(s) 1269
Ecl136II GAGCTC 2 cut(s) 229, 1065
Eco130I CCWWGG 1 cut(s) 1050
Eco147I AGGCCT 1 cut(s) 2078
Eco24I GRGCYC 4 cut(s) 231, 1067, 1934, 2109
Eco47I GGWCC 4 cut(s) 632, 791, 983, 1816
Eco53kI GAGCTC 2 cut(s) 229, 1065
Eco57I CTGAAG 4 cut(s) 605, 1185, 1226, 2196
Eco81I CCTNAGG 1 cut(s) 1284
EcoICRI GAGCTC 2 cut(s) 229, 1065
EcoO109I RGGNCCY 1 cut(s) 791
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 444, 702
EcoT14I CCWWGG 1 cut(s) 1050
EcoT22I ATGCAT 4 cut(s) 1403, 1797, 2440, 2647
EcoT38I GRGCYC 4 cut(s) 231, 1067, 1934, 2109
ErhI CCWWGG 1 cut(s) 1050
Esp3I CGTCTC 2 cut(s) 1220, 1385
FaeI CATG 8 cut(s) 619, 934, 1401, 1526, 1714, 2045, 2649, 2678
FalI AAGNNNNNCTT 4 cut(s) 728, 760, 2612, 2644
FaqI GGGAC 1 cut(s) 235
FatI CATG 8 cut(s) 615, 930, 1397, 1522, 1710, 2041, 2645, 2674
FauI CCCGC 3 cut(s) 46, 210, 1148
FbaI TGATCA 1 cut(s) 2544
Fnu4HI GCNGC 9 cut(s) 432, 528, 646, 663, 1160, 1811, 2284, 2650, 2705
FokI GGATG 4 cut(s) 617, 654, 893, 1033
FriOI GRGCYC 4 cut(s) 231, 1067, 1934, 2109
Fsp4HI GCNGC 9 cut(s) 432, 528, 646, 663, 1160, 1811, 2284, 2650, 2705
FspBI CTAG 1 cut(s) 1865
GlaI GCGC 1 cut(s) 1659
GluI GCNGC 9 cut(s) 432, 528, 646, 663, 1160, 1811, 2284, 2650, 2705
GsuI CTGGAG 3 cut(s) 402, 444, 801
HaeIII GGCC 2 cut(s) 1139, 2078
HapII CCGG 4 cut(s) 268, 1140, 1322, 1568
HgaI GACGC 1 cut(s) 253
HhaI GCGC 1 cut(s) 1660
Hin1I GRCGYC 1 cut(s) 1394
Hin1II CATG 8 cut(s) 619, 934, 1401, 1526, 1714, 2045, 2649, 2678
Hin6I GCGC 1 cut(s) 1658
HinP1I GCGC 1 cut(s) 1658
HindIII AAGCTT 4 cut(s) 195, 248, 516, 2213
HpaII CCGG 4 cut(s) 268, 1140, 1322, 1568
HphI GGTGA 3 cut(s) 613, 866, 2693
Hpy99I CGWCG 2 cut(s) 1514, 2021
HpyAV CCTTC 6 cut(s) 197, 292, 580, 996, 1451, 2089
HpyCH4III ACNGT 4 cut(s) 128, 1135, 1941, 2576
HpyCH4IV ACGT 3 cut(s) 1394, 1468, 1509
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 50, 524, 1243, 2113, 2857
HpySE526I ACGT 3 cut(s) 1394, 1468, 1509
Hsp92I GRCGYC 1 cut(s) 1394
Hsp92II CATG 8 cut(s) 619, 934, 1401, 1526, 1714, 2045, 2649, 2678
HspAI GCGC 1 cut(s) 1658
Kpn2I TCCGGA 1 cut(s) 267
Ksp22I TGATCA 1 cut(s) 2544
KspI CCGCGG 1 cut(s) 435
LmnI GCTCC 9 cut(s) 261, 577, 748, 1251, 1282, 1475, 1874, 1977, 2007
Lsp1109I GCAGC 6 cut(s) 539, 674, 1171, 1797, 2636, 2691
LweI GCATC 7 cut(s) 632, 1055, 1797, 1914, 2290, 2632, 2853
MaeI CTAG 1 cut(s) 1865
MaeII ACGT 3 cut(s) 1394, 1468, 1509
MaeIII GTNAC 5 cut(s) 721, 952, 1342, 2037, 2576
MbiI CCGCTC 1 cut(s) 1478
MfeI CAATTG 1 cut(s) 170
MflI RGATCY 5 cut(s) 175, 611, 859, 1564, 2023
MhlI GDGCHC 5 cut(s) 231, 1067, 1934, 2109, 2853
MlyI GAGTC 6 cut(s) 201, 329, 1897, 2234, 2378, 2572
MmeI TCCRAC 4 cut(s) 171, 538, 1179, 2568
Mph1103I ATGCAT 4 cut(s) 1403, 1797, 2440, 2647
MroI TCCGGA 1 cut(s) 267
MseI TTAA 9 cut(s) 521, 600, 2058, 2264, 2336, 2408, 2418, 2624, 2696
MslI CAYNNNNRTG 2 cut(s) 1133, 2679
MspA1I CMGCKG 2 cut(s) 434, 2502
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 4 cut(s) 268, 1140, 1322, 1568
MspR9I CCNGG 3 cut(s) 446, 704, 1323
MunI CAATTG 1 cut(s) 170
Mva1269I GAATGC 2 cut(s) 35, 1797
MvaI CCWGG 2 cut(s) 446, 704
MvnI CGCG 3 cut(s) 434, 1431, 1660
MwoI GCNNNNNNNGC 6 cut(s) 41, 50, 524, 1243, 2113, 2857
NciI CCSGG 1 cut(s) 1323
NlaIII CATG 8 cut(s) 619, 934, 1401, 1526, 1714, 2045, 2649, 2678
NlaIV GGNNCC 2 cut(s) 1566, 1870
NmuCI GTSAC 3 cut(s) 721, 952, 2037
NsiI ATGCAT 4 cut(s) 1403, 1797, 2440, 2647
NspI RCATGY 4 cut(s) 934, 2045, 2649, 2678
NspV TTCGAA 3 cut(s) 1088, 1534, 2294
PaeI GCATGC 1 cut(s) 2649
PaqCI CACCTGC 1 cut(s) 346
PceI AGGCCT 1 cut(s) 2078
PciI ACATGT 2 cut(s) 930, 2041
PctI GAATGC 2 cut(s) 35, 1797
PfeI GAWTC 5 cut(s) 595, 1948, 2312, 2528, 2744
PflMI CCANNNNNTGG 3 cut(s) 151, 172, 1626
PfoI TCCNGGA 2 cut(s) 702, 1321
PkrI GCNGC 9 cut(s) 433, 529, 647, 664, 1161, 1812, 2285, 2651, 2706
PleI GAGTC 6 cut(s) 201, 328, 1896, 2234, 2378, 2571
PpsI GAGTC 6 cut(s) 201, 328, 1896, 2234, 2378, 2571
PpuMI RGGWCCY 1 cut(s) 791
PscI ACATGT 2 cut(s) 930, 2041
PsiI TTATAA 1 cut(s) 786
Psp124BI GAGCTC 2 cut(s) 231, 1067
Psp1406I AACGTT 1 cut(s) 1468
Psp5II RGGWCCY 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 444, 702
PspGI CCWGG 2 cut(s) 444, 702
PspN4I GGNNCC 2 cut(s) 1566, 1870
PspPI GGNCC 4 cut(s) 632, 791, 983, 1816
PspPPI RGGWCCY 1 cut(s) 791
PstI CTGCAG 4 cut(s) 640, 2104, 2654, 2709
PsuI RGATCY 5 cut(s) 175, 611, 859, 1564, 2023
RsaI GTAC 6 cut(s) 293, 345, 926, 1482, 1938, 2573
RsaNI GTAC 6 cut(s) 292, 344, 925, 1481, 1937, 2572
RseI CAYNNNNRTG 2 cut(s) 1133, 2679
SacI GAGCTC 2 cut(s) 231, 1067
SacII CCGCGG 1 cut(s) 435
SaqAI TTAA 9 cut(s) 521, 600, 2058, 2264, 2336, 2408, 2418, 2624, 2696
SatI GCNGC 9 cut(s) 432, 528, 646, 663, 1160, 1811, 2284, 2650, 2705
Sau96I GGNCC 4 cut(s) 632, 791, 983, 1816
SbfI CCTGCAGG 2 cut(s) 640, 2104
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 6 cut(s) 201, 329, 1897, 2234, 2378, 2572
ScrFI CCNGG 3 cut(s) 446, 704, 1323
SdaI CCTGCAGG 2 cut(s) 640, 2104
SduI GDGCHC 5 cut(s) 231, 1067, 1934, 2109, 2853
SfaNI GCATC 7 cut(s) 632, 1055, 1797, 1914, 2290, 2632, 2853
SfcI CTRYAG 4 cut(s) 636, 2100, 2650, 2705
Sfr303I CCGCGG 1 cut(s) 435
SfuI TTCGAA 3 cut(s) 1088, 1534, 2294
SgrBI CCGCGG 1 cut(s) 435
SinI GGWCC 4 cut(s) 632, 791, 983, 1816
SmiMI CAYNNNNRTG 2 cut(s) 1133, 2679
SmlI CTYRAG 9 cut(s) 347, 599, 911, 1802, 2216, 2255, 2399, 2471, 2759
SmoI CTYRAG 9 cut(s) 347, 599, 911, 1802, 2216, 2255, 2399, 2471, 2759
SphI GCATGC 1 cut(s) 2649
Sse8387I CCTGCAGG 2 cut(s) 640, 2104
SseBI AGGCCT 1 cut(s) 2078
SspI AATATT 2 cut(s) 1106, 2056
SspMI CTAG 1 cut(s) 1865
SstI GAGCTC 2 cut(s) 231, 1067
StuI AGGCCT 1 cut(s) 2078
StyD4I CCNGG 3 cut(s) 444, 702, 1321
StyI CCWWGG 1 cut(s) 1050
TaaI ACNGT 4 cut(s) 128, 1135, 1941, 2576
TaiI ACGT 3 cut(s) 1397, 1471, 1512
TatI WGTACW 3 cut(s) 291, 924, 1936
TauI GCSGC 3 cut(s) 434, 648, 2286
TfiI GAWTC 5 cut(s) 595, 1948, 2312, 2528, 2744
Tru1I TTAA 9 cut(s) 521, 600, 2058, 2264, 2336, 2408, 2418, 2624, 2696
Tru9I TTAA 9 cut(s) 521, 600, 2058, 2264, 2336, 2408, 2418, 2624, 2696
TscAI CASTG 6 cut(s) 144, 537, 825, 1140, 1507, 1701
TseFI GTSAC 3 cut(s) 721, 952, 2037
TseI GCWGC 6 cut(s) 527, 662, 1159, 1810, 2649, 2704
Tsp45I GTSAC 3 cut(s) 721, 952, 2037
TspRI CASTG 6 cut(s) 144, 537, 825, 1140, 1507, 1701
Van91I CCANNNNNTGG 3 cut(s) 151, 172, 1626
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 4 cut(s) 632, 791, 983, 1816
XceI RCATGY 4 cut(s) 934, 2045, 2649, 2678
XcmI CCANNNNNNNNNTGG 1 cut(s) 2188
XspI CTAG 1 cut(s) 1865
ZraI GACGTC 1 cut(s) 1395
ZrmI AGTACT 1 cut(s) 293
Zsp2I ATGCAT 4 cut(s) 1403, 1797, 2440, 2647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.