FvH4_6g50330

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000039
Physical Location & Seq
Forward (+)
37483504 .. 37486439
2936 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50330.t1

Sequence Viewer

Length: 2085 bp
ATGGCAAACTTGGTTCTCGATAAAGCATTCGAAGTGCTGTGTGCTGGAGTTCAGGATCTCATTGTCAAGAATTTCATTTTCAAGTCTCTCCTCCACGACGCGAAATCCAGACTCGACAAGTTGGCACCACTGATCAAGAAGCTGGCGCATTATAACCAGATAATGGATGGCCAAGATCAGAAGGAACTGGAAGATTTAAAGGGAGTCCTGGAGGAGGGCCAAGAGCTCATCAATAAGTGTTCAACCGTTACACGATGGAGCCCCTTCAAAAAGTACAAGTACGCCAGACAAATTCTCCAGTGGGAAGAATCTCTGAAAAGCCCGTTGGAGAGTGTGAAGCTGCAAGAAGCAGTGAATGTAATGGACATGAGGATCGATATGCAACGCATGATGCTGAAACTACAACATTCTGGAGAAGGTTCGAGTACTAGAACACGACTTGAACTTCCACCACTTCCACAGCTTGTAGTTGGACTGGATGCTCCACTGAAGGACTTGAAGGAAAAGCTCCTTTGCGATGATGGGGGGTCGATGTTTGTGCTTACTGCTCCTGGAGCCTGCGGGAAAACTACTTTAGCCACAATGTTTTGTAAAGATGAAGAGGTCAAAGGTAAATTCAAGAACAATATTGTCTTCATTACTGTATCCCAAAATGGCAATTTGGAACTTGTTGTGAGACGACTTTGTGAATACGTGGGTTCTCCGGAATCCATTCTTGAGAGCTTTCGATTTGAAATGTCAAATTACAAGATTTTAGTGACATCAAGATCTGAATTTTCAAGATTTGGTTTTTCATATCATTTAAAGTTATTGGATGATGACAACGCTATGGAACTTTTTTATCACTCAGCCTCACTCAGAGATGAGCGCTCTCATACTCTAGAAGATCTCTCGAGAAAGATAGTAAAGCGTTGCAACGGATTTCCACTTGCGATTACAGTTATGGGAAGTTCACTCTCTGGGCAACCTTTAGAGAGCAGCCTAGATGCTTTGGATAAAGGATTTTCGCTTGTGAAAGATTGTTACTTAGACCTGGGATCTTTTCCTGAAGACAAAAAAATCCCGACGGAGGAGGACGATTACTACGGTCAACGGTTTGTTACACAACATGACTTGCTTAGAGAGCTAGCTATTTACAACGCAAAGATAGACCCGGTAGATCAAAGAAGAAGAATCATGTTAGAATTTTGTGGAAACAATATTCCCAAGTGGTGGAAGGAACAGAAATATCAACAAACCAATGCTCGCTTGTTATCCATCACAACTGATGGAGAATTCTCAATGAAATGGCCCCAGATTCAACTACCTGAAGCAGAGGTTCTTGTCTTGAATTTCGATATATACTCCTCATATCCCTTACCCGAATTTGTGGGGAAAATGGATAAACTGAAGGTAGTGGTAATCTTTTCTCGCAGTGGCCTTTGTAAGTTAAAAAATCTAGAACTGCTCCATCTCCTACCAAATCTCAAAAGAATCAGCTTGCATAGCATCCCGATAAATAGCTTAAGAAAGAATCCCATTCAACTGAAGAGTTTGAAGAAGATATCTTTAAGCTCATGTCACCCAATGGCATGTTTCAGCAAAGGTGTTGGCAAAATGTCGGATGCATTTCCAAATATAGAGGAATTAAACATTGACGATTGCAATGAGCCTGAAGAGTTGCTTGCGGATTTCTTTAATGTGATTCATTTAAAGAAAATCAGTATCTTTGACTGTTATGATTTACGTAGTTTGCCTGAAGAAATTGGAAATTTAGTCAACTTGGAAGTATTGAGGCTAAGGAAATGTAGTAACCTTTTAAAGTTTACTGGCTCAGTTAGAAACCTCAAAAAGTTAAATTTCCTCGACTTGTCTGGTTGTTACAACATTCAGTTGCCTGAAGACATTGGTGAACTTAGCAGTTTAAGAAAGTTGGACCTGAGGAGGTGCTACCAAGTGTGGAAGCTGCCACCATCAGTTTCGGATCTTGATCAGTTAGAGGAAGTGAGATGCGATGATTTTAGAGTCGATTTATGGGAAGCCTTTCTACCGACTCTCACAAACCTACGCATAGTGGGCAGTGGCGTAGCTAGAAACTCGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

695

Amino Acids

79.38

Weight (kDa)

6.64

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 135 4.4e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
LRR_14 PF23598 535 - 615 1.3e-06 Leucine-rich repeat region
LRR_14 PF23598 605 - 682 2.6e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 153
AccB1I GGYRCC 1 cut(s) 124
AccB7I CCANNNNNTGG 2 cut(s) 163, 1212
AccII CGCG 1 cut(s) 101
AccIII TCCGGA 1 cut(s) 703
AciI CCGC 2 cut(s) 561, 1667
AclWI GGATC 4 cut(s) 63, 380, 1045, 1971
AcoI YGGCCR 1 cut(s) 169
AcuI CTGAAG 8 cut(s) 509, 1068, 1329, 1409, 1547, 1674, 1758, 1899
AfaI GTAC 3 cut(s) 275, 281, 427
AfeI AGCGCT 1 cut(s) 869
AfiI CCNNNNNNNGG 4 cut(s) 163, 214, 1069, 1212
AflII CTTAAG 1 cut(s) 1504
AjnI CCWGG 3 cut(s) 207, 550, 1032
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 90, 670
AlwI GGATC 4 cut(s) 63, 380, 1045, 1971
Ama87I CYCGRG 1 cut(s) 892
Aor13HI TCCGGA 1 cut(s) 703
Aor51HI AGCGCT 1 cut(s) 869
AoxI GGCC 4 cut(s) 169, 217, 1289, 1417
ApeKI GCWGC 3 cut(s) 340, 978, 1945
Asp700I GAANNNNTTC 2 cut(s) 711, 2022
AspLEI GCGC 2 cut(s) 148, 870
AspS9I GGNCC 3 cut(s) 217, 1290, 1915
AsuC2I CCSGG 1 cut(s) 1154
AsuHPI GGTGA 2 cut(s) 1553, 1901
AsuII TTCGAA 1 cut(s) 30
AsuNHI GCTAGC 1 cut(s) 1126
AvaI CYCGRG 1 cut(s) 892
AvaII GGWCC 1 cut(s) 1915
AxyI CCTNAGG 1 cut(s) 1919
BalI TGGCCA 1 cut(s) 171
BanI GGYRCC 1 cut(s) 124
BanII GRGCYC 2 cut(s) 228, 263
BarI GAAGNNNNNNTAC 2 cut(s) 2010, 2042
BbsI GAAGAC 3 cut(s) 625, 1056, 1887
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 3 cut(s) 327, 990, 1932
BccI CCATC 7 cut(s) 161, 249, 515, 1262, 1265, 1458, 1960
BcgI CGANNNNNNTGC 2 cut(s) 520, 554
BciT130I CCWGG 3 cut(s) 209, 552, 1034
BciVI GTATCC 1 cut(s) 655
BclI TGATCA 2 cut(s) 132, 1969
BcnI CCSGG 1 cut(s) 1154
BcoDI GTCTC 2 cut(s) 90, 670
BfaI CTAG 6 cut(s) 429, 881, 983, 1127, 1439, 2070
BfoI RGCGCY 1 cut(s) 871
BfrI CTTAAG 1 cut(s) 1504
BfuI GTATCC 1 cut(s) 655
BglII AGATCT 2 cut(s) 767, 886
BisI GCNGC 3 cut(s) 341, 979, 1946
BlsI GCNGC 3 cut(s) 342, 980, 1947
BmcAI AGTACT 1 cut(s) 427
Bme1390I CCNGG 4 cut(s) 209, 552, 1034, 1154
Bme18I GGWCC 1 cut(s) 1915
BmeT110I CYCGRG 1 cut(s) 892
BmgT120I GGNCC 3 cut(s) 217, 1290, 1915
BmiI GGNNCC 4 cut(s) 126, 260, 556, 1292
BmrFI CCNGG 4 cut(s) 209, 552, 1034, 1154
BmsI GCATC 6 cut(s) 381, 469, 976, 1497, 1594, 1979
BmtI GCTAGC 1 cut(s) 1130
BpiI GAAGAC 3 cut(s) 625, 1056, 1887
BpmI CTGGAG 5 cut(s) 66, 230, 281, 432, 573
Bpu10I CCTNAGC 1 cut(s) 1778
Bpu14I TTCGAA 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 1 cut(s) 1154
Bsa29I ATCGAT 1 cut(s) 375
BsaAI YACGTR 2 cut(s) 694, 1727
BsaBI GATNNNNATC 1 cut(s) 1968
BsaJI CCNNGG 1 cut(s) 1033
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 214, 1069, 1212
Bse1I ACTGG 4 cut(s) 192, 298, 480, 1813
Bse21I CCTNAGG 1 cut(s) 1919
Bse3DI GCAATG 1 cut(s) 1651
Bse8I GATNNNNATC 1 cut(s) 1968
BseAI TCCGGA 1 cut(s) 703
BseBI CCWGG 3 cut(s) 209, 552, 1034
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 1033
BseGI GGATG 5 cut(s) 172, 484, 820, 1488, 1609
BseJI GATNNNNATC 1 cut(s) 1968
BseLI CCNNNNNNNGG 4 cut(s) 163, 214, 1069, 1212
BseMI GCAATG 1 cut(s) 1651
BseMII CTCAG 4 cut(s) 861, 871, 1827, 1910
BseNI ACTGG 4 cut(s) 192, 298, 480, 1813
BseRI GAGGAG 5 cut(s) 80, 227, 1085, 1336, 1936
BseXI GCAGC 3 cut(s) 327, 990, 1932
Bsh1236I CGCG 1 cut(s) 101
BshFI GGCC 4 cut(s) 171, 219, 1291, 1419
BshNI GGYRCC 1 cut(s) 124
BshVI ATCGAT 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 892
BsiSI CCGG 2 cut(s) 704, 1154
BslI CCNNNNNNNGG 4 cut(s) 163, 214, 1069, 1212
BsmAI GTCTC 2 cut(s) 90, 670
BsmBI CGTCTC 1 cut(s) 670
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 4 cut(s) 171, 219, 1291, 1419
BsoBI CYCGRG 1 cut(s) 892
Bsp119I TTCGAA 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 228, 263
Bsp13I TCCGGA 1 cut(s) 703
BspACI CCGC 2 cut(s) 561, 1667
BspANI GGCC 4 cut(s) 171, 219, 1291, 1419
BspCNI CTCAG 4 cut(s) 860, 870, 1826, 1911
BspDI ATCGAT 1 cut(s) 375
BspEI TCCGGA 1 cut(s) 703
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 4 cut(s) 126, 260, 556, 1292
BspOI GCTAGC 1 cut(s) 1130
BspPI GGATC 4 cut(s) 63, 380, 1045, 1971
BspT104I TTCGAA 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 124
BspTI CTTAAG 1 cut(s) 1504
BsrDI GCAATG 1 cut(s) 1651
BsrI ACTGG 4 cut(s) 192, 298, 480, 1813
BssECI CCNNGG 1 cut(s) 1033
Bst2UI CCWGG 3 cut(s) 209, 552, 1034
Bst4CI ACNGT 6 cut(s) 247, 643, 940, 1088, 1095, 1715
Bst6I CTCTTC 3 cut(s) 594, 1523, 1650
BstAFI CTTAAG 1 cut(s) 1504
BstBAI YACGTR 2 cut(s) 694, 1727
BstBI TTCGAA 1 cut(s) 30
BstC8I GCNNGC 6 cut(s) 144, 559, 1128, 1246, 1481, 1665
BstDEI CTNAG 8 cut(s) 847, 857, 1027, 1118, 1778, 1813, 1895, 1919
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 5 cut(s) 172, 484, 820, 1488, 1609
BstFNI CGCG 1 cut(s) 101
BstH2I RGCGCY 1 cut(s) 871
BstHHI GCGC 2 cut(s) 148, 870
BstMAI GTCTC 2 cut(s) 90, 670
BstMWI GCNNNNNNNGC 4 cut(s) 554, 1123, 1485, 2055
BstNI CCWGG 3 cut(s) 209, 552, 1034
BstNSI RCATGY 1 cut(s) 1575
BstSCI CCNGG 4 cut(s) 207, 550, 1032, 1152
BstSNI TACGTA 1 cut(s) 1727
BstUI CGCG 1 cut(s) 101
BstV1I GCAGC 3 cut(s) 327, 990, 1932
BstV2I GAAGAC 3 cut(s) 625, 1056, 1887
BstX2I RGATCY 5 cut(s) 55, 767, 886, 1037, 1963
BstYI RGATCY 5 cut(s) 55, 767, 886, 1037, 1963
Bsu15I ATCGAT 1 cut(s) 375
Bsu36I CCTNAGG 1 cut(s) 1919
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 4 cut(s) 171, 219, 1291, 1419
BsuTUI ATCGAT 1 cut(s) 375
BtgZI GCGATG 2 cut(s) 531, 2007
BtsCI GGATG 5 cut(s) 172, 484, 820, 1488, 1609
BtsI GCAGTG 3 cut(s) 357, 1420, 2065
BtsIMutI CAGTG 6 cut(s) 128, 305, 357, 485, 1420, 2065
Cac8I GCNNGC 6 cut(s) 144, 559, 1128, 1246, 1481, 1665
CfoI GCGC 2 cut(s) 148, 870
Cfr13I GGNCC 3 cut(s) 217, 1290, 1915
ClaI ATCGAT 1 cut(s) 375
CseI GACGC 1 cut(s) 107
Csp6I GTAC 3 cut(s) 274, 280, 426
CviAII CATG 6 cut(s) 367, 388, 1109, 1177, 1557, 1572
CviQI GTAC 3 cut(s) 274, 280, 426
DdeI CTNAG 8 cut(s) 847, 857, 1027, 1118, 1778, 1813, 1895, 1919
DraI TTTAAA 4 cut(s) 198, 804, 1692, 1800
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 3 cut(s) 594, 1523, 1650
EarI CTCTTC 3 cut(s) 594, 1523, 1650
Ecl136II GAGCTC 1 cut(s) 226
Eco105I TACGTA 1 cut(s) 1727
Eco24I GRGCYC 2 cut(s) 228, 263
Eco32I GATATC 1 cut(s) 1545
Eco47I GGWCC 1 cut(s) 1915
Eco47III AGCGCT 1 cut(s) 869
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 8 cut(s) 509, 1068, 1329, 1409, 1547, 1674, 1758, 1899
Eco81I CCTNAGG 1 cut(s) 1919
Eco88I CYCGRG 1 cut(s) 892
EcoICRI GAGCTC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoRI GAATTC 1 cut(s) 1274
EcoRII CCWGG 3 cut(s) 207, 550, 1032
EcoRV GATATC 1 cut(s) 1545
EcoT22I ATGCAT 1 cut(s) 1609
EcoT38I GRGCYC 2 cut(s) 228, 263
Esp3I CGTCTC 1 cut(s) 670
FaeI CATG 6 cut(s) 370, 391, 1112, 1180, 1560, 1575
FalI AAGNNNNNCTT 2 cut(s) 1647, 1679
FatI CATG 6 cut(s) 366, 387, 1108, 1176, 1556, 1571
FauI CCCGC 1 cut(s) 554
FbaI TGATCA 2 cut(s) 132, 1969
Fnu4HI GCNGC 3 cut(s) 341, 979, 1946
FokI GGATG 5 cut(s) 179, 491, 827, 1475, 1616
FriOI GRGCYC 2 cut(s) 228, 263
Fsp4HI GCNGC 3 cut(s) 341, 979, 1946
FspBI CTAG 6 cut(s) 429, 881, 983, 1127, 1439, 2070
GlaI GCGC 2 cut(s) 147, 869
GluI GCNGC 3 cut(s) 341, 979, 1946
GsuI CTGGAG 5 cut(s) 66, 230, 281, 432, 573
HaeII RGCGCY 1 cut(s) 871
HaeIII GGCC 4 cut(s) 171, 219, 1291, 1419
HapII CCGG 2 cut(s) 704, 1154
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 148, 870
Hin1II CATG 6 cut(s) 370, 391, 1112, 1180, 1560, 1575
Hin6I GCGC 2 cut(s) 146, 868
HinP1I GCGC 2 cut(s) 146, 868
HincII GTYRAC 2 cut(s) 1091, 1759
HindII GTYRAC 2 cut(s) 1091, 1759
HpaII CCGG 2 cut(s) 704, 1154
HphI GGTGA 2 cut(s) 1553, 1901
Hpy166II GTNNAC 5 cut(s) 953, 1091, 1759, 1806, 1892
Hpy188I TCNGA 6 cut(s) 180, 315, 772, 860, 1603, 1963
Hpy8I GTNNAC 5 cut(s) 953, 1091, 1759, 1806, 1892
Hpy99I CGWCG 2 cut(s) 101, 1069
HpyAV CCTTC 7 cut(s) 175, 274, 410, 484, 493, 1210, 1384
HpyCH4III ACNGT 6 cut(s) 247, 643, 940, 1088, 1095, 1715
HpyCH4IV ACGT 2 cut(s) 693, 1726
HpyCH4V TGCA 6 cut(s) 343, 382, 915, 1483, 1607, 1644
HpyF10VI GCNNNNNNNGC 4 cut(s) 554, 1123, 1485, 2055
HpyF3I CTNAG 8 cut(s) 847, 857, 1027, 1118, 1778, 1813, 1895, 1919
HpySE526I ACGT 2 cut(s) 693, 1726
Hsp92II CATG 6 cut(s) 370, 391, 1112, 1180, 1560, 1575
HspAI GCGC 2 cut(s) 146, 868
Kpn2I TCCGGA 1 cut(s) 703
Ksp22I TGATCA 2 cut(s) 132, 1969
LmnI GCTCC 6 cut(s) 258, 487, 513, 553, 554, 1452
Lsp1109I GCAGC 3 cut(s) 327, 990, 1932
LweI GCATC 6 cut(s) 381, 469, 976, 1497, 1594, 1979
MaeI CTAG 6 cut(s) 429, 881, 983, 1127, 1439, 2070
MaeII ACGT 2 cut(s) 693, 1726
MaeIII GTNAC 7 cut(s) 247, 757, 1022, 1099, 1559, 1790, 1859
MflI RGATCY 5 cut(s) 55, 767, 886, 1037, 1963
MhlI GDGCHC 2 cut(s) 228, 263
MlsI TGGCCA 1 cut(s) 171
MluNI TGGCCA 1 cut(s) 171
MlyI GAGTC 4 cut(s) 105, 213, 2013, 2026
MmeI TCCRAC 4 cut(s) 306, 451, 1581, 1893
Mox20I TGGCCA 1 cut(s) 171
Mph1103I ATGCAT 1 cut(s) 1609
MroI TCCGGA 1 cut(s) 703
MroXI GAANNNNTTC 2 cut(s) 711, 2022
MscI TGGCCA 1 cut(s) 171
Msp20I TGGCCA 1 cut(s) 171
MspCI CTTAAG 1 cut(s) 1504
MspI CCGG 2 cut(s) 704, 1154
MspR9I CCNGG 4 cut(s) 209, 552, 1034, 1154
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 3 cut(s) 209, 552, 1034
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 4 cut(s) 554, 1123, 1485, 2055
NciI CCSGG 1 cut(s) 1154
NheI GCTAGC 1 cut(s) 1126
NlaIII CATG 6 cut(s) 370, 391, 1112, 1180, 1560, 1575
NlaIV GGNNCC 4 cut(s) 126, 260, 556, 1292
NmuCI GTSAC 2 cut(s) 757, 1559
NsiI ATGCAT 1 cut(s) 1609
NspI RCATGY 1 cut(s) 1575
NspV TTCGAA 1 cut(s) 30
PaeR7I CTCGAG 1 cut(s) 892
PctI GAATGC 1 cut(s) 26
PdmI GAANNNNTTC 2 cut(s) 711, 2022
PfeI GAWTC 7 cut(s) 308, 707, 1173, 1297, 1473, 1513, 1684
PflMI CCANNNNNTGG 2 cut(s) 163, 1212
PfoI TCCNGGA 2 cut(s) 207, 550
PkrI GCNGC 3 cut(s) 342, 980, 1947
PleI GAGTC 4 cut(s) 105, 212, 2012, 2026
PpsI GAGTC 4 cut(s) 105, 212, 2012, 2026
Ppu21I YACGTR 2 cut(s) 694, 1727
PsiI TTATAA 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 207, 550, 1032
PspGI CCWGG 3 cut(s) 207, 550, 1032
PspN4I GGNNCC 4 cut(s) 126, 260, 556, 1292
PspPI GGNCC 3 cut(s) 217, 1290, 1915
PsuI RGATCY 5 cut(s) 55, 767, 886, 1037, 1963
RsaI GTAC 3 cut(s) 275, 281, 427
RsaNI GTAC 3 cut(s) 274, 280, 426
SacI GAGCTC 1 cut(s) 228
SatI GCNGC 3 cut(s) 341, 979, 1946
Sau96I GGNCC 3 cut(s) 217, 1290, 1915
ScaI AGTACT 1 cut(s) 427
SchI GAGTC 4 cut(s) 105, 213, 2013, 2026
ScrFI CCNGG 4 cut(s) 209, 552, 1034, 1154
SduI GDGCHC 2 cut(s) 228, 263
SfaNI GCATC 6 cut(s) 381, 469, 976, 1497, 1594, 1979
Sfr274I CTCGAG 1 cut(s) 892
SfuI TTCGAA 1 cut(s) 30
SinI GGWCC 1 cut(s) 1915
SlaI CTCGAG 1 cut(s) 892
SmlI CTYRAG 3 cut(s) 716, 892, 1504
SmoI CTYRAG 3 cut(s) 716, 892, 1504
SnaBI TACGTA 1 cut(s) 1727
SsiI CCGC 2 cut(s) 561, 1667
SspI AATATT 2 cut(s) 628, 1201
SspMI CTAG 6 cut(s) 429, 881, 983, 1127, 1439, 2070
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 4 cut(s) 207, 550, 1032, 1152
TaaI ACNGT 6 cut(s) 247, 643, 940, 1088, 1095, 1715
TaiI ACGT 2 cut(s) 696, 1729
TatI WGTACW 2 cut(s) 273, 425
TfiI GAWTC 7 cut(s) 308, 707, 1173, 1297, 1473, 1513, 1684
TscAI CASTG 6 cut(s) 135, 305, 357, 492, 1420, 2065
TseFI GTSAC 2 cut(s) 757, 1559
TseI GCWGC 3 cut(s) 340, 978, 1945
Tsp45I GTSAC 2 cut(s) 757, 1559
TspDTI ATGAA 6 cut(s) 64, 612, 625, 783, 1298, 1676
TspGWI ACGGA 2 cut(s) 933, 1082
TspRI CASTG 6 cut(s) 135, 305, 357, 492, 1420, 2065
Van91I CCANNNNNTGG 2 cut(s) 163, 1212
Vha464I CTTAAG 1 cut(s) 1504
VpaK11BI GGWCC 1 cut(s) 1915
XagI CCTNNNNNAGG 1 cut(s) 212
XbaI TCTAGA 2 cut(s) 880, 1438
XceI RCATGY 1 cut(s) 1575
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XhoI CTCGAG 1 cut(s) 892
XmnI GAANNNNTTC 2 cut(s) 711, 2022
XspI CTAG 6 cut(s) 429, 881, 983, 1127, 1439, 2070
ZrmI AGTACT 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 1609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.