Rroxscaffold_2G00139050

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000039
Physical Location & Seq
Reverse (-)
77317493 .. 77321367
3875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139050.1

Sequence Viewer

Length: 2517 bp
ATGATCAATCCACTGGATAAACTTTTCAGTGATCTCTGCGATGGCTTTAAAGACCTCATCAAGAAGAATGTTCAATTTAAAGGCAGAGTCGACGGCATCCAAGACAAGCTAAAAGCTCTAGAGCCGCTGATCCGAAACATGCTGCGGAATAATGAGGCATTAGATCGCCCAAGGAACGAACTAAAAGATTTAACAGAAGTTATGGAGTCTGGCATCAAGCTCACTGTCAAGTGCTCAAAGTCTCCGTCGTGGAAGCTCTTCAGCAGGTACAAATACGCCATCAGACTTACTGAATGGGAGAAACGTCTAGATGGTCAGTTACTTATGCTGAATGTGCACACCGCCAGCGATGTGAAGGAGATTGCGGTGGTTGTGAAGAATGTGGAGACTTTGGTAGGGAAGAATGCGCCCACCGCCAGCGATGTGAAGGAGATTGCGGCGGTTGTGAAGAATGTGGAGACTTTGGTGGGGAATATAGAGGAGAAATTGGAAATTGTGATACAAAATCCACCTGGGGAACCTAAGGCCTGGATGAGTACTGAAGAACGAGAACCTGAAGTTCCACGTTTTACAGTGGGATTGGACAAGCATTTGAAGGAATTGAAGATGAAGCTTCTCAAGGATGGGGGGTCAATGCTTGTAATAACAGCTCCTGGAGGGTCCGGCAAAACCACTTTGGCTACAAAGTTTTATCATGAAGTCACAGATAAATTCCCCAAGAACATCTTTGTTACTGTTTCAACAAAGTCCAACTGCATGGTTGTACAAGAACTATGTCAAAAATTGGGATCCCAGGAATCTGCATTCCCAAATGAAGACATTGCATTCAGCAAGCTGAAGGAATTTCTGAAGGAAACCGCACAAAAGCCTTTGCTTCTCATCCTGGATGATGTTTTGTCTGAATCAGAATCCCTTCTTGAGAAGTTTAATGAACCAAGTATACAATCAAATCTCAGTATCTTGGTGACATCAAGATCTTCATTTCCAAGTGTCGGTTCTCAATATCCTTTAGATGCATTGAATCACACAGATGCAATGACTCTTTTTCGCCATTCAGCAATCCGGGGAGAGAAGAGCTATAATGTTTCAGAAAATCTTCAGGACAAGATAGTAAGGCGCTGTAAGGGATGTCCACTAGCCATTAAAGTGGTCGGAAAATCTCTTTGTGGAAAATTTTCAGTGTCATTTCTTAAAAAATTAGACGAGTGGTCTGAAGGTGCTTCTATTTTTGATACCGAGACTGAAGTGCTTCTTTGCCTCAAAAGTAGCTTAGACGCCTTGGATGAAAAAGATCAAACTATCAAGGAATGCTTCTTGGACCTGGGTGCATTTCCTGAAGATCAAAGAATCCCAGCTGTTGCTCTGATTGATATGCGGGCAGAGCTATATGGCACAAAAGATTTTCTTTCCATTGAAAAGCTCCAGGAGCTTTCCAGACGAAGCCTGGCTAATCTTGTAGTCACAAGGAAGGAAAAGAAGGAGGCGGATGGCTACTACAGTGAACACTTTGTCTCCCAGCATGATCTGCTCAGGGATCTAGCTATCTTCCAAGCGAAATTAGATCCAAATAAAACGAGACTGATTTTAGACAAATGTGGAGTCAAAGTACCCAAGAAGCTGACAGAACAGAAGCATCGACCCTTTCAGACTCGCATATTATCCATCTCTGATGGAGTGTTCCCAACAGATATGCACAAGATTCAACTTTCAGATGTTGAGGTTCTAGTTTTGAATTTTCAGTCAGAGAACTATGCTTTACCCAAATTTGTGGCGGACATGGAAAAGTTGAAGGTCCTAATAGTCACAAATAATGGTTCCTTACCAGCTGAATTGAGTAACTTTGAACTACTGGATTCCTTGCCAAATCTGAAGAGAATCAGACTAGAGCGAATTTCAATTCCTTCCATAACCAAGAATCGCATGCAGTTGAAGAGTGTAAAGAAGATCTCTTTATTCATGTGTAGCATCAGTCAAGCTTTCAGCAACCCTTCCTTCCAAATTTCATATGCATTTCCAAATCTTGAGGAATTGAACATTGACTATTGCAGTGACTTGGTGGAATTATATGCTGATCTCAGTGATCTGATTGAGCTAAAGAATCTCAGTATCACCCACTGTCACAAGCTATCTGCCTTGCCTGAAGAGATAGGAAATTTGATTAAATTGGAAGTCTTGAGGCTAAAGTCATGTTCAGACTTGGTAACATTTCCAGCCTCAATTAAGAACCTTGGAAAGTTGCAGTTTCTCGATATATCTGATTGCTTCAGCATCAAGGAGTTGCCTGAAGACATTGGTGAAATCAGCACTTTAAAAAAGATCAATATGAGACAGTGCTCTAGACTGAGGGATCTACCTGAATCAGTTTTTGATCTCGAGCAACTAGAGGAAGTGATATGTGACGAGGAAACGAAAAAGTGGTGGGAGCTTTTCGGGCTCAAAATAGAGATAAGGGTCCTCAAAGAAGAATACTACCTAGATTGGCTTCAAAGTTTCAATCTTGAAAGATACCTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

838

Amino Acids

95.24

Weight (kDa)

5.99

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 131 8.6e-14 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 195 - 327 9e-16 NB-ARC domain
AAA_22 PF13401 209 - 307 1.8e-06 AAA domain
LRR_14 PF23598 647 - 753 3.8e-09 Leucine-rich repeat region
LRR_14 PF23598 739 - 804 3.6e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
AccI GTMKAC 2 cut(s) 90, 940
AclWI GGATC 6 cut(s) 124, 783, 796, 1542, 1556, 2355
AcsI RAATTY 8 cut(s) 710, 842, 1172, 1732, 1763, 1890, 1998, 2152
AcyI GRCGYC 1 cut(s) 1275
AfaI GTAC 4 cut(s) 269, 538, 765, 1608
AfiI CCNNNNNNNGG 1 cut(s) 992
AhdI GACNNNNNGTC 1 cut(s) 1207
AjnI CCWGG 8 cut(s) 511, 527, 652, 792, 882, 1320, 1422, 1443
AjuI GAANNNNNNNTTGG 2 cut(s) 979, 1011
Alw21I GWGCWC 3 cut(s) 236, 339, 2336
Alw26I GTCTC 7 cut(s) 246, 380, 452, 1232, 1516, 1570, 2320
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 6 cut(s) 124, 783, 796, 1542, 1556, 2355
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 2372
AoxI GGCC 1 cut(s) 525
ApaLI GTGCAC 1 cut(s) 335
ApeKI GCWGC 1 cut(s) 142
ApoI RAATTY 8 cut(s) 710, 842, 1172, 1732, 1763, 1890, 1998, 2152
ArsI GACNNNNNNTTYG 4 cut(s) 230, 262, 1584, 1616
Asp700I GAANNNNTTC 5 cut(s) 257, 912, 1095, 1174, 1248
AspLEI GCGC 2 cut(s) 409, 1119
AspS9I GGNCC 4 cut(s) 660, 1318, 1792, 2452
AsuC2I CCSGG 1 cut(s) 1064
AsuHPI GGTGA 3 cut(s) 976, 2101, 2306
AvaI CYCGRG 1 cut(s) 2372
AvaII GGWCC 4 cut(s) 660, 1318, 1792, 2452
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 339
BaeI ACNNNNGTAYC 1 cut(s) 2497
BamHI GGATCC 1 cut(s) 788
BanII GRGCYC 1 cut(s) 2436
BbsI GAAGAC 2 cut(s) 822, 2292
Bbv12I GWGCWC 3 cut(s) 236, 339, 2336
BbvI GCAGC 1 cut(s) 129
BccI CCATC 7 cut(s) 35, 287, 305, 617, 1481, 1664, 1670
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 8 cut(s) 513, 529, 654, 794, 884, 1322, 1424, 1445
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 1064
BcoDI GTCTC 7 cut(s) 246, 380, 452, 1232, 1516, 1570, 2320
BfaI CTAG 9 cut(s) 119, 308, 1136, 1538, 1724, 1883, 2337, 2381, 2474
BfmI CTRYAG 1 cut(s) 1495
BfoI RGCGCY 1 cut(s) 1120
BfuAI ACCTGC 1 cut(s) 255
BglII AGATCT 2 cut(s) 974, 1944
BisI GCNGC 3 cut(s) 125, 143, 438
BlsI GCNGC 3 cut(s) 126, 144, 439
BmcAI AGTACT 1 cut(s) 538
Bme1390I CCNGG 9 cut(s) 513, 529, 654, 794, 884, 1064, 1322, 1424, 1445
Bme18I GGWCC 4 cut(s) 660, 1318, 1792, 2452
BmeRI GACNNNNNGTC 1 cut(s) 1207
BmeT110I CYCGRG 1 cut(s) 2372
BmgT120I GGNCC 4 cut(s) 660, 1318, 1792, 2452
BmiI GGNNCC 5 cut(s) 519, 661, 790, 1816, 2453
BmrFI CCNGG 9 cut(s) 513, 529, 654, 794, 884, 1064, 1322, 1424, 1445
BmsI GCATC 7 cut(s) 105, 222, 1003, 1021, 1642, 1974, 2277
BpiI GAAGAC 2 cut(s) 822, 2292
BpmI CTGGAG 2 cut(s) 675, 1406
Bpu10I CCTNAGC 1 cut(s) 1529
BpuEI CTTGAG 4 cut(s) 602, 938, 2042, 2194
BpuMI CCSGG 1 cut(s) 1064
BsaHI GRCGYC 1 cut(s) 1275
BsaJI CCNNGG 7 cut(s) 170, 512, 792, 1063, 1278, 1321, 2227
BsaXI ACNNNNNCTCC 4 cut(s) 1496, 1526, 1590, 1620
Bsc4I CCNNNNNNNGG 1 cut(s) 992
Bse1I ACTGG 2 cut(s) 18, 1854
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 2 cut(s) 819, 1041
BseBI CCWGG 8 cut(s) 513, 529, 654, 794, 884, 1322, 1424, 1445
BseDI CCNNGG 7 cut(s) 170, 512, 792, 1063, 1278, 1321, 2227
BseGI GGATG 8 cut(s) 96, 537, 628, 879, 892, 1133, 1288, 1492
BseLI CCNNNNNNNGG 1 cut(s) 992
BseMI GCAATG 2 cut(s) 819, 1041
BseMII CTCAG 5 cut(s) 967, 1543, 2089, 2116, 2333
BseNI ACTGG 2 cut(s) 18, 1854
BseRI GAGGAG 1 cut(s) 494
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 1 cut(s) 129
BseYI CCCAGC 2 cut(s) 1351, 1515
BshFI GGCC 1 cut(s) 527
BsiHKAI GWGCWC 3 cut(s) 236, 339, 2336
BsiHKCI CYCGRG 1 cut(s) 2372
BsiSI CCGG 2 cut(s) 663, 1063
BslI CCNNNNNNNGG 1 cut(s) 992
BsmAI GTCTC 7 cut(s) 246, 380, 452, 1232, 1516, 1570, 2320
BsmI GAATGC 4 cut(s) 409, 803, 824, 1313
BsnI GGCC 1 cut(s) 527
BsoBI CYCGRG 1 cut(s) 2372
Bsp1286I GDGCHC 4 cut(s) 236, 339, 2336, 2436
Bsp1407I TGTACA 1 cut(s) 763
BspANI GGCC 1 cut(s) 527
BspCNI CTCAG 5 cut(s) 966, 1542, 2088, 2115, 2334
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 5 cut(s) 519, 661, 790, 1816, 2453
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 6 cut(s) 124, 783, 796, 1542, 1556, 2355
BspQI GCTCTTC 2 cut(s) 263, 1067
BsrDI GCAATG 2 cut(s) 819, 1041
BsrGI TGTACA 1 cut(s) 763
BsrI ACTGG 2 cut(s) 18, 1854
BssECI CCNNGG 7 cut(s) 170, 512, 792, 1063, 1278, 1321, 2227
BssNAI GTATAC 1 cut(s) 941
BssNI GRCGYC 1 cut(s) 1275
BssT1I CCWWGG 3 cut(s) 170, 1278, 2227
Bst1107I GTATAC 1 cut(s) 941
Bst2UI CCWGG 8 cut(s) 513, 529, 654, 794, 884, 1322, 1424, 1445
Bst4CI ACNGT 6 cut(s) 226, 574, 736, 1499, 2117, 2331
Bst6I CTCTTC 5 cut(s) 263, 1067, 1865, 1925, 2136
BstACI GRCGYC 1 cut(s) 1275
BstAPI GCANNNNNTGC 1 cut(s) 1525
BstAUI TGTACA 1 cut(s) 763
BstC8I GCNNGC 5 cut(s) 346, 418, 833, 1377, 1922
BstDEI CTNAG 7 cut(s) 522, 953, 1270, 1529, 2075, 2102, 2342
BstF5I GGATG 8 cut(s) 96, 537, 628, 879, 892, 1133, 1288, 1492
BstH2I RGCGCY 1 cut(s) 1120
BstHHI GCGC 2 cut(s) 409, 1119
BstMAI GTCTC 7 cut(s) 246, 380, 452, 1232, 1516, 1570, 2320
BstMWI GCNNNNNNNGC 6 cut(s) 334, 413, 1381, 1426, 1525, 2431
BstNI CCWGG 8 cut(s) 513, 529, 654, 794, 884, 1322, 1424, 1445
BstNSI RCATGY 2 cut(s) 142, 1924
BstSCI CCNGG 9 cut(s) 511, 527, 652, 792, 882, 1062, 1320, 1422, 1443
BstSFI CTRYAG 1 cut(s) 1495
BstSLI GKGCMC 1 cut(s) 339
BstV1I GCAGC 1 cut(s) 129
BstV2I GAAGAC 2 cut(s) 822, 2292
BstX2I RGATCY 6 cut(s) 788, 974, 1534, 1561, 1944, 2347
BstXI CCANNNNNNTGG 3 cut(s) 757, 1147, 1768
BstYI RGATCY 6 cut(s) 788, 974, 1534, 1561, 1944, 2347
BstZ17I GTATAC 1 cut(s) 941
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 1 cut(s) 527
BtgZI GCGATG 3 cut(s) 54, 363, 435
BtsCI GGATG 8 cut(s) 96, 537, 628, 879, 892, 1133, 1288, 1492
BtsI GCAGTG 1 cut(s) 2053
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 5 cut(s) 346, 418, 833, 1377, 1922
CaiI CAGNNNCTG 1 cut(s) 653
CciI TCATGA 1 cut(s) 694
CfoI GCGC 2 cut(s) 409, 1119
Cfr13I GGNCC 4 cut(s) 660, 1318, 1792, 2452
CseI GACGC 1 cut(s) 1283
Csp6I GTAC 4 cut(s) 268, 537, 764, 1607
CviAII CATG 8 cut(s) 139, 695, 757, 1520, 1777, 1921, 1957, 2187
CviQI GTAC 4 cut(s) 268, 537, 764, 1607
DdeI CTNAG 7 cut(s) 522, 953, 1270, 1529, 2075, 2102, 2342
DraI TTTAAA 3 cut(s) 49, 79, 2310
DriI GACNNNNNGTC 1 cut(s) 1207
Eam1104I CTCTTC 5 cut(s) 263, 1067, 1865, 1925, 2136
Eam1105I GACNNNNNGTC 1 cut(s) 1207
EarI CTCTTC 5 cut(s) 263, 1067, 1865, 1925, 2136
EciI GGCGGA 2 cut(s) 1499, 1787
Eco130I CCWWGG 3 cut(s) 170, 1278, 2227
Eco147I AGGCCT 1 cut(s) 527
Eco24I GRGCYC 1 cut(s) 2436
Eco47I GGWCC 4 cut(s) 660, 1318, 1792, 2452
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 2372
EcoO109I RGGNCCY 2 cut(s) 1792, 2452
EcoRII CCWGG 8 cut(s) 511, 527, 652, 792, 882, 1320, 1422, 1443
EcoT14I CCWWGG 3 cut(s) 170, 1278, 2227
EcoT22I ATGCAT 2 cut(s) 1018, 2011
EcoT38I GRGCYC 1 cut(s) 2436
ErhI CCWWGG 3 cut(s) 170, 1278, 2227
FaeI CATG 8 cut(s) 142, 698, 760, 1523, 1780, 1924, 1960, 2190
FalI AAGNNNNNCTT 8 cut(s) 710, 742, 1236, 1268, 1295, 1327, 1389, 1421
FatI CATG 8 cut(s) 138, 694, 756, 1519, 1776, 1920, 1956, 2186
FauI CCCGC 1 cut(s) 1368
FauNDI CATATG 1 cut(s) 2005
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 90, 940
Fnu4HI GCNGC 3 cut(s) 125, 143, 438
FokI GGATG 8 cut(s) 83, 544, 635, 866, 899, 1140, 1295, 1499
FriOI GRGCYC 1 cut(s) 2436
Fsp4HI GCNGC 3 cut(s) 125, 143, 438
FspBI CTAG 9 cut(s) 119, 308, 1136, 1538, 1724, 1883, 2337, 2381, 2474
GlaI GCGC 2 cut(s) 408, 1118
GluI GCNGC 3 cut(s) 125, 143, 438
GsaI CCCAGC 2 cut(s) 1355, 1519
GsuI CTGGAG 2 cut(s) 675, 1406
HaeII RGCGCY 1 cut(s) 1120
HaeIII GGCC 1 cut(s) 527
HapII CCGG 2 cut(s) 663, 1063
HgaI GACGC 1 cut(s) 1283
HhaI GCGC 2 cut(s) 409, 1119
Hin1I GRCGYC 1 cut(s) 1275
Hin1II CATG 8 cut(s) 142, 698, 760, 1523, 1780, 1924, 1960, 2190
Hin6I GCGC 2 cut(s) 407, 1117
HinP1I GCGC 2 cut(s) 407, 1117
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 2 cut(s) 611, 1974
HpaII CCGG 2 cut(s) 663, 1063
HphI GGTGA 3 cut(s) 976, 2101, 2306
Hpy166II GTNNAC 5 cut(s) 91, 337, 941, 1133, 1502
Hpy8I GTNNAC 5 cut(s) 91, 337, 941, 1133, 1502
Hpy99I CGWCG 2 cut(s) 95, 250
HpyCH4III ACNGT 6 cut(s) 226, 574, 736, 1499, 2117, 2331
HpyCH4IV ACGT 2 cut(s) 304, 565
HpyF10VI GCNNNNNNNGC 6 cut(s) 334, 413, 1381, 1426, 1525, 2431
HpyF3I CTNAG 7 cut(s) 522, 953, 1270, 1529, 2075, 2102, 2342
HpySE526I ACGT 2 cut(s) 304, 565
Hsp92I GRCGYC 1 cut(s) 1275
Hsp92II CATG 8 cut(s) 142, 698, 760, 1523, 1780, 1924, 1960, 2190
HspAI GCGC 2 cut(s) 407, 1117
Ksp22I TGATCA 1 cut(s) 3
LguI GCTCTTC 2 cut(s) 263, 1067
LmnI GCTCC 4 cut(s) 655, 1425, 1426, 2422
Lsp1109I GCAGC 1 cut(s) 129
LweI GCATC 7 cut(s) 105, 222, 1003, 1021, 1642, 1974, 2277
MaeI CTAG 9 cut(s) 119, 308, 1136, 1538, 1724, 1883, 2337, 2381, 2474
MaeII ACGT 2 cut(s) 304, 565
MflI RGATCY 6 cut(s) 788, 974, 1534, 1561, 1944, 2347
MhlI GDGCHC 4 cut(s) 236, 339, 2336, 2436
MlyI GAGTC 5 cut(s) 96, 215, 1033, 1608, 1642
MmeI TCCRAC 2 cut(s) 774, 1132
Mph1103I ATGCAT 2 cut(s) 1018, 2011
MroXI GAANNNNTTC 5 cut(s) 257, 912, 1095, 1174, 1248
MslI CAYNNNNRTG 2 cut(s) 1029, 1145
MspA1I CMGCKG 3 cut(s) 127, 1355, 1826
MspI CCGG 2 cut(s) 663, 1063
MspR9I CCNGG 9 cut(s) 513, 529, 654, 794, 884, 1064, 1322, 1424, 1445
Mva1269I GAATGC 4 cut(s) 409, 803, 824, 1313
MvaI CCWGG 8 cut(s) 513, 529, 654, 794, 884, 1322, 1424, 1445
MwoI GCNNNNNNNGC 6 cut(s) 334, 413, 1381, 1426, 1525, 2431
NciI CCSGG 1 cut(s) 1064
NdeI CATATG 1 cut(s) 2005
NlaIII CATG 8 cut(s) 142, 698, 760, 1523, 1780, 1924, 1960, 2190
NlaIV GGNNCC 5 cut(s) 519, 661, 790, 1816, 2453
NmuCI GTSAC 7 cut(s) 700, 964, 1459, 1801, 2048, 2117, 2396
NsiI ATGCAT 2 cut(s) 1018, 2011
NspI RCATGY 2 cut(s) 142, 1924
PaeI GCATGC 1 cut(s) 1924
PaeR7I CTCGAG 1 cut(s) 2372
PagI TCATGA 1 cut(s) 694
PceI AGGCCT 1 cut(s) 527
PciSI GCTCTTC 2 cut(s) 263, 1067
PctI GAATGC 4 cut(s) 409, 803, 824, 1313
PdmI GAANNNNTTC 5 cut(s) 257, 912, 1095, 1174, 1248
PfoI TCCNGGA 3 cut(s) 652, 882, 1422
PkrI GCNGC 3 cut(s) 126, 144, 439
PleI GAGTC 5 cut(s) 95, 214, 1033, 1607, 1642
PpsI GAGTC 5 cut(s) 95, 214, 1033, 1607, 1642
PpuMI RGGWCCY 2 cut(s) 1792, 2452
Psp5II RGGWCCY 2 cut(s) 1792, 2452
Psp6I CCWGG 8 cut(s) 511, 527, 652, 792, 882, 1320, 1422, 1443
PspFI CCCAGC 2 cut(s) 1351, 1515
PspGI CCWGG 8 cut(s) 511, 527, 652, 792, 882, 1320, 1422, 1443
PspN4I GGNNCC 5 cut(s) 519, 661, 790, 1816, 2453
PspPI GGNCC 4 cut(s) 660, 1318, 1792, 2452
PspPPI RGGWCCY 2 cut(s) 1792, 2452
PsrI GAACNNNNNNTAC 2 cut(s) 924, 956
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 6 cut(s) 788, 974, 1534, 1561, 1944, 2347
PvuII CAGCTG 2 cut(s) 1355, 1826
RsaI GTAC 4 cut(s) 269, 538, 765, 1608
RsaNI GTAC 4 cut(s) 268, 537, 764, 1607
RseI CAYNNNNRTG 2 cut(s) 1029, 1145
SalI GTCGAC 1 cut(s) 89
SapI GCTCTTC 2 cut(s) 263, 1067
SatI GCNGC 3 cut(s) 125, 143, 438
Sau96I GGNCC 4 cut(s) 660, 1318, 1792, 2452
ScaI AGTACT 1 cut(s) 538
SchI GAGTC 5 cut(s) 96, 215, 1033, 1608, 1642
ScrFI CCNGG 9 cut(s) 513, 529, 654, 794, 884, 1064, 1322, 1424, 1445
SduI GDGCHC 4 cut(s) 236, 339, 2336, 2436
SfaNI GCATC 7 cut(s) 105, 222, 1003, 1021, 1642, 1974, 2277
SfcI CTRYAG 1 cut(s) 1495
Sfr274I CTCGAG 1 cut(s) 2372
SinI GGWCC 4 cut(s) 660, 1318, 1792, 2452
SlaI CTCGAG 1 cut(s) 2372
SmiMI CAYNNNNRTG 2 cut(s) 1029, 1145
SmlI CTYRAG 5 cut(s) 617, 917, 2021, 2173, 2372
SmoI CTYRAG 5 cut(s) 617, 917, 2021, 2173, 2372
SphI GCATGC 1 cut(s) 1924
SseBI AGGCCT 1 cut(s) 527
SspMI CTAG 9 cut(s) 119, 308, 1136, 1538, 1724, 1883, 2337, 2381, 2474
StuI AGGCCT 1 cut(s) 527
StyD4I CCNGG 9 cut(s) 511, 527, 652, 792, 882, 1062, 1320, 1422, 1443
StyI CCWWGG 3 cut(s) 170, 1278, 2227
TaaI ACNGT 6 cut(s) 226, 574, 736, 1499, 2117, 2331
TaiI ACGT 2 cut(s) 307, 568
TaqI TCGA 4 cut(s) 90, 1636, 2247, 2373
TatI WGTACW 2 cut(s) 536, 763
TauI GCSGC 2 cut(s) 127, 440
TseFI GTSAC 7 cut(s) 700, 964, 1459, 1801, 2048, 2117, 2396
TseI GCWGC 1 cut(s) 142
Tsp45I GTSAC 7 cut(s) 700, 964, 1459, 1801, 2048, 2117, 2396
TspDTI ATGAA 8 cut(s) 623, 711, 828, 945, 969, 1299, 1945, 1992
TspGWI ACGGA 1 cut(s) 234
VneI GTGCAC 1 cut(s) 335
VpaK11BI GGWCC 4 cut(s) 660, 1318, 1792, 2452
XapI RAATTY 8 cut(s) 710, 842, 1172, 1732, 1763, 1890, 1998, 2152
XbaI TCTAGA 3 cut(s) 118, 307, 2336
XceI RCATGY 2 cut(s) 142, 1924
XcmI CCANNNNNNNNNTGG 1 cut(s) 1441
XhoI CTCGAG 1 cut(s) 2372
XmiI GTMKAC 2 cut(s) 90, 940
XmnI GAANNNNTTC 5 cut(s) 257, 912, 1095, 1174, 1248
XspI CTAG 9 cut(s) 119, 308, 1136, 1538, 1724, 1883, 2337, 2381, 2474
ZrmI AGTACT 1 cut(s) 538
Zsp2I ATGCAT 2 cut(s) 1018, 2011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.