FvH4_1g15220

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000039
Physical Location & Seq
Forward (+)
8637945 .. 8643108
5164 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15220.t4

Sequence Viewer

Length: 2445 bp
ATGGCAGTGGCGTTTGATGGACAAGCAGTTTTCCAAGCATTATTCAATGTTGTCAGTGAAGTGATTGAGAAGAATTCAATGTTTAAATCCCAGCTTGTAGATATCAAATCAGCTCTAGACTGTTTAGAACCACTGATTGAAGTGATACCAGAGTACGGAAAGGAATTGGATCGGCAAGAGGAGGAACTACAGAAGTTTACAATACAAATGAAGGAGGGAATTCAGCTGATTCACAAGTGCTCCAAAGTTCATGCGTGGAATGCATACAAGAGACACAAATACGCAAATGAACTTGTTGAATTGGATAAATCTCTACAAAGGCTGATGGATATACTGAAAGTGCTTGGCATCAGAGATGCGAAGAAAACACTGGCTACAGTAAAGAATATTGAGACTAGGTTCCAGCAGATTGATGAGAAATTTGTGGTACAAAATATTCCATCTAAAAAAATTGAAGCTTGGTCGGCAGTACCTGAATTACCACCTCTTACAGTTGGACTGGAGGGGCCTTTGAAAGAATTGAAGCTAATGCTTCTTAAGGATGGGTTATCAATGCTTGTGCTCACTGCTCCAGGAGGATGCGGGAAAACTACATTGGCAACTAAGTTTTGTCAAGATGCAGAAGTGCGAGACAAGTTCAAGAACAATATCTTCTTTGTAACGGTATCAAAGAAGCCCAACTTAAACCTTATTGTACAAGAGCTTTGTCAACGCAAGGGTTCCCAGGTACCGGATTTCCAAACTGACGTAAGTGCAGTCGACTGGCTGCAAGAATTCGTAAAGCAAACAGATCCTTTGCTGTTGGTTTTGGATGATATTTGGTCTGAATCCGAATCCCTTCTTGAGAAGCTTGATGAATTGAAAATTGCAAATCACAAGATTCTGGGGACATCTAGATTTGTATTTCCAAGATTTTGTCCTCCATATTATTTAGAGTCCTTGAATGAAAAAGATGCAACAGCACTTTTTTATCATTCAGCATCATTGGGAGATAGGAGCTCTCATGTTCCAGAAGACCTTTCAAGAAAGATAGTAGAACATTGTAAGGGATTTCCACTTGCCATTACAGTGATTGGAAGATCACTTTGTGGGCAGCCTTTGGAGATCTGGCAAAAAAGAGTAATTGAATGGACTAAAGGTTCATCCATTCTTGATTCTGATCATCATTTGCTTGCTTGTCTCCAAAGCAGCTTAGATGCCTTGGAGAAAGAAAATAGTGTCATCAAGGAATGTTTCATGGATCTTGGTTCATTTCCTGAGGACCAAAGAATATCTGCTACTATCCTCATTGATATATGGTCGGTGTTATATAAGATAGAGGAAGACATGCTTTGCATTGGAAACGTTGAGGAACTCAACAATAGGAGTTTGGCCACTCTTGTAGTCACAAGGAAGGACAACAAGGAGGTGGATGGATATTACGCTGAACATTTTGTTACTCAGCATGACATTCTTAGAGAATTGGCTATCTATCAGGCAAGTCTAGGGTCAATAGAACAAAGAAAAAGGGTAATAATAGATATCTGTGGAGATAATCTTCCCGAATGGTGGATAAAACAGAAGTATCAACCCATCAGCGCTCGCCTTGTATCTATCTCAACAGATGGAGACTTCTCAGCTACATGGCCTAACATGGAATTACCACAAGCAGAGGTTCTAATTCTGAATTTTCAGTCAAAGAAATATGCCTTACCCGAATTTCTGGAGAAAGTGGATAAATTAAAGGTTCTGATAATCACAAATTATGGTGTTTTACCTGCTCAGATAAATAATTTTCCGTTACTTAGGTCTTTATCAAATCTAAAGAGGATCAGGTTAGAACGAATCTCAATACCTTCCATAAGCAAGGACCCTGTGCAGTTGGATAATTTGGAAAATATATCTTTATTCATGTGCAACGTCAGTAAAGCTTTTAGCAACTGTTCCAGCCAACTTGTAGATCTATTGCCAAATGTGGTGGAAATAAACATTGATTATTGCAATGATTTGGTGGAACTGCCTGCCATGCTTTGTGACCTGATTCCCCTTAAAAAGCTTAGTATCACCAATTCTCACAAGCTATCTGCCTTACCTGAAGAAATAGGAAAGTTAGTGAACTTAGAAGTGTTGAGGGTCAGGTCTTGTACAGACTTGTCGGTGCTGCCAGACTCAATTCGGCACCTCAAAATGTTAAGTTTTCTGGACATATCTGATTGCTATAGTATGAAGGAGCTCCCTGAACACATTGGTGAAATGTGCAGCTTAGAAACGCTAAATATGAGACAATGCTCAAGACTGCAAGAGTTGCCTGAATCAGTGTTGGATCTTGAACAGTTGGAGGATGTGATTTGTGATGAAGAGACTGAAATATTATGGAAACCGTTCTTGCCCATTCTCCAAAACATACATATCAGGGTAGTGAAAGAAGATATTAACTTGAATTGGCTCCACAAGTTTCCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

815

Amino Acids

92.85

Weight (kDa)

5.27

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 134 2.8e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 170 - 284 5.4e-13 NB-ARC domain
AAA_22 PF13401 184 - 288 6.9e-07 AAA domain
LRR_14 PF23598 669 - 732 1.8e-08 Leucine-rich repeat region
LRR_14 PF23598 724 - 783 4.2e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1765
Acc65I GGTACC 1 cut(s) 727
AccB1I GGYRCC 2 cut(s) 727, 2157
AccI GTMKAC 1 cut(s) 759
AciI CCGC 1 cut(s) 582
AclI AACGTT 1 cut(s) 1344
AclWI GGATC 5 cut(s) 177, 785, 1248, 1817, 2310
AcoI YGGCCR 1 cut(s) 1371
AcsI RAATTY 6 cut(s) 73, 219, 419, 773, 1666, 1697
AcuI CTGAAG 1 cut(s) 2094
AdeI CACNNNGTG 1 cut(s) 1088
AfaI GTAC 6 cut(s) 155, 429, 471, 696, 729, 2125
AfeI AGCGCT 1 cut(s) 1579
AfiI CCNNNNNNNGG 3 cut(s) 155, 730, 1548
AflII CTTAAG 1 cut(s) 536
AjnI CCWGG 2 cut(s) 571, 723
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AleI CACNNNNGTG 1 cut(s) 2226
AloI GAACNNNNNNTCC 2 cut(s) 1123, 1155
Alw21I GWGCWC 4 cut(s) 242, 564, 1001, 2214
Alw26I GTCTC 7 cut(s) 265, 386, 624, 1184, 1602, 2254, 2333
AlwI GGATC 5 cut(s) 177, 785, 1248, 1817, 2310
AlwNI CAGNNNCTG 1 cut(s) 473
Aor51HI AGCGCT 1 cut(s) 1579
AoxI GGCC 3 cut(s) 506, 1371, 1625
ApeKI GCWGC 5 cut(s) 766, 1093, 1188, 2140, 2238
ApoI RAATTY 6 cut(s) 73, 219, 419, 773, 1666, 1697
Asp700I GAANNNNTTC 2 cut(s) 837, 2360
Asp718I GGTACC 1 cut(s) 727
AspLEI GCGC 1 cut(s) 1580
AspS9I GGNCC 3 cut(s) 506, 1261, 1849
AsuHPI GGTGA 2 cut(s) 2035, 2240
AvaII GGWCC 2 cut(s) 1261, 1849
AxyI CCTNAGG 1 cut(s) 1257
BaeI ACNNNNGTAYC 4 cut(s) 137, 170, 1547, 1580
BalI TGGCCA 1 cut(s) 1373
BanI GGYRCC 2 cut(s) 727, 2157
BanII GRGCYC 2 cut(s) 1001, 2214
BbsI GAAGAC 2 cut(s) 1020, 1329
Bbv12I GWGCWC 4 cut(s) 242, 564, 1001, 2214
BbvI GCAGC 5 cut(s) 753, 1105, 1200, 2127, 2250
BccI CCATC 7 cut(s) 11, 319, 448, 536, 1406, 1580, 1598
BciT130I CCWGG 2 cut(s) 573, 725
BclI TGATCA 1 cut(s) 1159
BcoDI GTCTC 7 cut(s) 265, 386, 624, 1184, 1602, 2254, 2333
BfaI CTAG 4 cut(s) 116, 396, 894, 1484
BfmI CTRYAG 3 cut(s) 188, 375, 2197
BfoI RGCGCY 1 cut(s) 1581
BfrI CTTAAG 1 cut(s) 536
BfuAI ACCTGC 1 cut(s) 1765
BglII AGATCT 2 cut(s) 1104, 1939
BisI GCNGC 5 cut(s) 767, 1094, 1189, 2141, 2239
BlsI GCNGC 5 cut(s) 768, 1095, 1190, 2142, 2240
Bme1390I CCNGG 2 cut(s) 573, 725
Bme18I GGWCC 2 cut(s) 1261, 1849
BmgT120I GGNCC 3 cut(s) 506, 1261, 1849
BmiI GGNNCC 7 cut(s) 401, 507, 721, 729, 1851, 2159, 2426
BmrFI CCNGG 2 cut(s) 573, 725
BmsI GCATC 7 cut(s) 346, 357, 569, 607, 943, 989, 1186
BpiI GAAGAC 2 cut(s) 1020, 1329
BpmI CTGGAG 3 cut(s) 521, 555, 1724
BpuEI CTTGAG 2 cut(s) 863, 2254
BsaBI GATNNNNATC 1 cut(s) 1158
BsaJI CCNNGG 2 cut(s) 723, 1200
BsaWI WCCGGW 1 cut(s) 730
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 730, 1548
Bse1I ACTGG 3 cut(s) 375, 504, 767
Bse21I CCTNAGG 1 cut(s) 1257
Bse3DI GCAATG 1 cut(s) 1987
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 2 cut(s) 573, 725
BseDI CCNNGG 2 cut(s) 723, 1200
BseGI GGATG 6 cut(s) 547, 584, 817, 1142, 1417, 2326
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 3 cut(s) 155, 730, 1548
BseMI GCAATG 1 cut(s) 1987
BseMII CTCAG 4 cut(s) 1248, 1454, 1629, 1775
BseNI ACTGG 3 cut(s) 375, 504, 767
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 5 cut(s) 753, 1105, 1200, 2127, 2250
BseYI CCCAGC 1 cut(s) 90
BsgI GTGCAG 3 cut(s) 774, 1877, 2257
BshFI GGCC 3 cut(s) 508, 1373, 1627
BshNI GGYRCC 2 cut(s) 727, 2157
BsiHKAI GWGCWC 4 cut(s) 242, 564, 1001, 2214
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 3 cut(s) 155, 730, 1548
BsmAI GTCTC 7 cut(s) 265, 386, 624, 1184, 1602, 2254, 2333
BsmFI GGGAC 1 cut(s) 901
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 3 cut(s) 508, 1373, 1627
Bsp1286I GDGCHC 4 cut(s) 242, 564, 1001, 2214
Bsp1407I TGTACA 2 cut(s) 694, 2123
Bsp143I GATC 9 cut(s) 169, 790, 1079, 1104, 1159, 1240, 1809, 1939, 2302
BspACI CCGC 1 cut(s) 582
BspANI GGCC 3 cut(s) 508, 1373, 1627
BspCNI CTCAG 4 cut(s) 1249, 1453, 1628, 1774
BspLI GGNNCC 7 cut(s) 401, 507, 721, 729, 1851, 2159, 2426
BspMI ACCTGC 1 cut(s) 1765
BspPI GGATC 5 cut(s) 177, 785, 1248, 1817, 2310
BspT107I GGYRCC 2 cut(s) 727, 2157
BspTI CTTAAG 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 1987
BsrGI TGTACA 2 cut(s) 694, 2123
BsrI ACTGG 3 cut(s) 375, 504, 767
BssECI CCNNGG 2 cut(s) 723, 1200
BssMI GATC 9 cut(s) 169, 790, 1079, 1104, 1159, 1240, 1809, 1939, 2302
BssT1I CCWWGG 1 cut(s) 1200
Bst2UI CCWGG 2 cut(s) 573, 725
Bst4CI ACNGT 8 cut(s) 122, 379, 493, 664, 1069, 1922, 2313, 2361
Bst6I CTCTTC 1 cut(s) 2331
BstAFI CTTAAG 1 cut(s) 536
BstAPI GCANNNNNTGC 1 cut(s) 2284
BstAUI TGTACA 2 cut(s) 694, 2123
BstC8I GCNNGC 3 cut(s) 1173, 1582, 2001
BstF5I GGATG 6 cut(s) 547, 584, 817, 1142, 1417, 2326
BstH2I RGCGCY 1 cut(s) 1581
BstHHI GCGC 1 cut(s) 1580
BstKTI GATC 9 cut(s) 172, 793, 1082, 1107, 1162, 1243, 1812, 1942, 2305
BstMAI GTCTC 7 cut(s) 265, 386, 624, 1184, 1602, 2254, 2333
BstMBI GATC 9 cut(s) 169, 790, 1079, 1104, 1159, 1240, 1809, 1939, 2302
BstMWI GCNNNNNNNGC 4 cut(s) 260, 464, 2005, 2284
BstNI CCWGG 2 cut(s) 573, 725
BstNSI RCATGY 1 cut(s) 1330
BstSCI CCNGG 2 cut(s) 571, 723
BstSFI CTRYAG 3 cut(s) 188, 375, 2197
BstV1I GCAGC 5 cut(s) 753, 1105, 1200, 2127, 2250
BstV2I GAAGAC 2 cut(s) 1020, 1329
BstX2I RGATCY 5 cut(s) 790, 1104, 1240, 1939, 2302
BstYI RGATCY 5 cut(s) 790, 1104, 1240, 1939, 2302
Bsu36I CCTNAGG 1 cut(s) 1257
BsuRI GGCC 3 cut(s) 508, 1373, 1627
BtsCI GGATG 6 cut(s) 547, 584, 817, 1142, 1417, 2326
BtsI GCAGTG 2 cut(s) 12, 564
BtsIMutI CAGTG 7 cut(s) 12, 61, 131, 368, 564, 1074, 2301
BveI ACCTGC 1 cut(s) 1765
Cac8I GCNNGC 3 cut(s) 1173, 1582, 2001
CaiI CAGNNNCTG 1 cut(s) 473
CfoI GCGC 1 cut(s) 1580
Cfr13I GGNCC 3 cut(s) 506, 1261, 1849
Csp6I GTAC 6 cut(s) 154, 428, 470, 695, 728, 2124
CspCI CAANNNNNGTGG 2 cut(s) 1938, 1973
CviAII CATG 9 cut(s) 251, 1004, 1237, 1327, 1445, 1623, 1633, 1891, 2005
CviQI GTAC 6 cut(s) 154, 428, 470, 695, 728, 2124
DpnI GATC 9 cut(s) 171, 792, 1081, 1106, 1161, 1242, 1811, 1941, 2304
DpnII GATC 9 cut(s) 169, 790, 1079, 1104, 1159, 1240, 1809, 1939, 2302
DraI TTTAAA 1 cut(s) 85
DraIII CACNNNGTG 1 cut(s) 1088
EaeI YGGCCR 1 cut(s) 1371
Eam1104I CTCTTC 1 cut(s) 2331
EarI CTCTTC 1 cut(s) 2331
Ecl136II GAGCTC 2 cut(s) 999, 2212
Eco130I CCWWGG 1 cut(s) 1200
Eco24I GRGCYC 2 cut(s) 1001, 2214
Eco32I GATATC 2 cut(s) 103, 1522
Eco47I GGWCC 2 cut(s) 1261, 1849
Eco47III AGCGCT 1 cut(s) 1579
Eco53kI GAGCTC 2 cut(s) 999, 2212
Eco57I CTGAAG 1 cut(s) 2094
Eco81I CCTNAGG 1 cut(s) 1257
EcoICRI GAGCTC 2 cut(s) 999, 2212
EcoO109I RGGNCCY 2 cut(s) 506, 1849
EcoRI GAATTC 3 cut(s) 73, 219, 773
EcoRII CCWGG 2 cut(s) 571, 723
EcoRV GATATC 2 cut(s) 103, 1522
EcoT14I CCWWGG 1 cut(s) 1200
EcoT22I ATGCAT 1 cut(s) 265
EcoT38I GRGCYC 2 cut(s) 1001, 2214
ErhI CCWWGG 1 cut(s) 1200
FaeI CATG 9 cut(s) 254, 1007, 1240, 1330, 1448, 1626, 1636, 1894, 2008
FalI AAGNNNNNCTT 4 cut(s) 665, 697, 1314, 1346
FaqI GGGAC 1 cut(s) 901
FatI CATG 9 cut(s) 250, 1003, 1236, 1326, 1444, 1622, 1632, 1890, 2004
FauI CCCGC 1 cut(s) 575
FbaI TGATCA 1 cut(s) 1159
FblI GTMKAC 1 cut(s) 759
Fnu4HI GCNGC 5 cut(s) 767, 1094, 1189, 2141, 2239
FokI GGATG 6 cut(s) 554, 591, 824, 1129, 1424, 2333
FriOI GRGCYC 2 cut(s) 1001, 2214
Fsp4HI GCNGC 5 cut(s) 767, 1094, 1189, 2141, 2239
FspBI CTAG 4 cut(s) 116, 396, 894, 1484
GlaI GCGC 1 cut(s) 1579
GluI GCNGC 5 cut(s) 767, 1094, 1189, 2141, 2239
GsaI CCCAGC 1 cut(s) 94
GsuI CTGGAG 3 cut(s) 521, 555, 1724
HaeII RGCGCY 1 cut(s) 1581
HaeIII GGCC 3 cut(s) 508, 1373, 1627
HapII CCGG 1 cut(s) 731
HhaI GCGC 1 cut(s) 1580
Hin1II CATG 9 cut(s) 254, 1007, 1240, 1330, 1448, 1626, 1636, 1894, 2008
Hin6I GCGC 1 cut(s) 1578
HinP1I GCGC 1 cut(s) 1578
HincII GTYRAC 2 cut(s) 710, 760
HindII GTYRAC 2 cut(s) 710, 760
HindIII AAGCTT 4 cut(s) 456, 848, 1908, 2033
HpaII CCGG 1 cut(s) 731
HphI GGTGA 2 cut(s) 2035, 2240
Hpy166II GTNNAC 4 cut(s) 198, 710, 760, 2095
Hpy188I TCNGA 8 cut(s) 353, 826, 832, 1159, 1665, 1731, 1764, 2191
Hpy8I GTNNAC 4 cut(s) 198, 710, 760, 2095
HpyAV CCTTC 5 cut(s) 205, 848, 1387, 1845, 2200
HpyCH4III ACNGT 8 cut(s) 122, 379, 493, 664, 1069, 1922, 2313, 2361
HpyCH4IV ACGT 3 cut(s) 747, 1344, 1899
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 464, 2005, 2284
HpySE526I ACGT 3 cut(s) 747, 1344, 1899
Hsp92II CATG 9 cut(s) 254, 1007, 1240, 1330, 1448, 1626, 1636, 1894, 2008
HspAI GCGC 1 cut(s) 1578
KpnI GGTACC 1 cut(s) 731
Ksp22I TGATCA 1 cut(s) 1159
Kzo9I GATC 9 cut(s) 169, 790, 1079, 1104, 1159, 1240, 1809, 1939, 2302
LmnI GCTCC 6 cut(s) 245, 574, 996, 2209, 2217, 2430
Lsp1109I GCAGC 5 cut(s) 753, 1105, 1200, 2127, 2250
LweI GCATC 7 cut(s) 346, 357, 569, 607, 943, 989, 1186
MaeI CTAG 4 cut(s) 116, 396, 894, 1484
MaeII ACGT 3 cut(s) 747, 1344, 1899
MaeIII GTNAC 5 cut(s) 658, 1384, 1435, 1779, 2012
MalI GATC 9 cut(s) 171, 792, 1081, 1106, 1161, 1242, 1811, 1941, 2304
MboI GATC 9 cut(s) 169, 790, 1079, 1104, 1159, 1240, 1809, 1939, 2302
MflI RGATCY 5 cut(s) 790, 1104, 1240, 1939, 2302
MhlI GDGCHC 4 cut(s) 242, 564, 1001, 2214
MlsI TGGCCA 1 cut(s) 1373
MluNI TGGCCA 1 cut(s) 1373
MlyI GAGTC 2 cut(s) 944, 2141
MmeI TCCRAC 4 cut(s) 475, 1842, 2280, 2295
Mox20I TGGCCA 1 cut(s) 1373
Mph1103I ATGCAT 1 cut(s) 265
MroXI GAANNNNTTC 2 cut(s) 837, 2360
MscI TGGCCA 1 cut(s) 1373
MseI TTAA 8 cut(s) 84, 537, 683, 1721, 2028, 2171, 2412, 2443
MslI CAYNNNNRTG 2 cut(s) 1067, 2226
Msp20I TGGCCA 1 cut(s) 1373
MspA1I CMGCKG 1 cut(s) 226
MspCI CTTAAG 1 cut(s) 536
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 2 cut(s) 573, 725
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 2 cut(s) 573, 725
MwoI GCNNNNNNNGC 4 cut(s) 260, 464, 2005, 2284
NdeII GATC 9 cut(s) 169, 790, 1079, 1104, 1159, 1240, 1809, 1939, 2302
NlaIII CATG 9 cut(s) 254, 1007, 1240, 1330, 1448, 1626, 1636, 1894, 2008
NlaIV GGNNCC 7 cut(s) 401, 507, 721, 729, 1851, 2159, 2426
NmuCI GTSAC 2 cut(s) 1384, 2012
NsiI ATGCAT 1 cut(s) 265
NspI RCATGY 1 cut(s) 1330
OliI CACNNNNGTG 1 cut(s) 2226
PctI GAATGC 1 cut(s) 265
PdmI GAANNNNTTC 2 cut(s) 837, 2360
PfeI GAWTC 8 cut(s) 229, 827, 833, 880, 1154, 1824, 2020, 2291
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 5 cut(s) 768, 1095, 1190, 2142, 2240
PleI GAGTC 2 cut(s) 943, 2141
PpsI GAGTC 2 cut(s) 943, 2141
PpuMI RGGWCCY 1 cut(s) 1849
Psp124BI GAGCTC 2 cut(s) 1001, 2214
Psp1406I AACGTT 1 cut(s) 1344
Psp5II RGGWCCY 1 cut(s) 1849
Psp6I CCWGG 2 cut(s) 571, 723
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 2 cut(s) 571, 723
PspN4I GGNNCC 7 cut(s) 401, 507, 721, 729, 1851, 2159, 2426
PspPI GGNCC 3 cut(s) 506, 1261, 1849
PspPPI RGGWCCY 1 cut(s) 1849
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 5 cut(s) 790, 1104, 1240, 1939, 2302
PvuII CAGCTG 1 cut(s) 226
RsaI GTAC 6 cut(s) 155, 429, 471, 696, 729, 2125
RsaNI GTAC 6 cut(s) 154, 428, 470, 695, 728, 2124
RseI CAYNNNNRTG 2 cut(s) 1067, 2226
SacI GAGCTC 2 cut(s) 1001, 2214
SalI GTCGAC 1 cut(s) 758
SaqAI TTAA 8 cut(s) 84, 537, 683, 1721, 2028, 2171, 2412, 2443
SatI GCNGC 5 cut(s) 767, 1094, 1189, 2141, 2239
Sau3AI GATC 9 cut(s) 169, 790, 1079, 1104, 1159, 1240, 1809, 1939, 2302
Sau96I GGNCC 3 cut(s) 506, 1261, 1849
SchI GAGTC 2 cut(s) 944, 2141
ScrFI CCNGG 2 cut(s) 573, 725
SduI GDGCHC 4 cut(s) 242, 564, 1001, 2214
SfaNI GCATC 7 cut(s) 346, 357, 569, 607, 943, 989, 1186
SfcI CTRYAG 3 cut(s) 188, 375, 2197
SinI GGWCC 2 cut(s) 1261, 1849
SmiMI CAYNNNNRTG 2 cut(s) 1067, 2226
SmlI CTYRAG 3 cut(s) 536, 842, 2269
SmoI CTYRAG 3 cut(s) 536, 842, 2269
SsiI CCGC 1 cut(s) 582
SspI AATATT 3 cut(s) 388, 436, 2349
SspMI CTAG 4 cut(s) 116, 396, 894, 1484
SstI GAGCTC 2 cut(s) 1001, 2214
StyD4I CCNGG 2 cut(s) 571, 723
StyI CCWWGG 1 cut(s) 1200
TaaI ACNGT 8 cut(s) 122, 379, 493, 664, 1069, 1922, 2313, 2361
TaiI ACGT 3 cut(s) 750, 1347, 1902
TaqI TCGA 1 cut(s) 759
TatI WGTACW 2 cut(s) 694, 2123
TfiI GAWTC 8 cut(s) 229, 827, 833, 880, 1154, 1824, 2020, 2291
Tru1I TTAA 8 cut(s) 84, 537, 683, 1721, 2028, 2171, 2412, 2443
Tru9I TTAA 8 cut(s) 84, 537, 683, 1721, 2028, 2171, 2412, 2443
TscAI CASTG 7 cut(s) 12, 61, 138, 375, 571, 1074, 2301
TseFI GTSAC 2 cut(s) 1384, 2012
TseI GCWGC 5 cut(s) 766, 1093, 1188, 2140, 2238
Tsp45I GTSAC 2 cut(s) 1384, 2012
TspGWI ACGGA 2 cut(s) 171, 1767
TspRI CASTG 7 cut(s) 12, 61, 138, 375, 571, 1074, 2301
Vha464I CTTAAG 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 1261, 1849
XapI RAATTY 6 cut(s) 73, 219, 419, 773, 1666, 1697
XbaI TCTAGA 2 cut(s) 115, 893
XceI RCATGY 1 cut(s) 1330
XmiI GTMKAC 1 cut(s) 759
XmnI GAANNNNTTC 2 cut(s) 837, 2360
XspI CTAG 4 cut(s) 116, 396, 894, 1484
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.