MD15G1278600.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000039
Physical Location & Seq
Forward (+)
24621206 .. 24624806
3601 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1278600.v1.1.491

Sequence Viewer

Length: 2421 bp
ATGGCAGTCCTTGCGTCAGCTGTAGCAGCTATTGTAAAAATACCATTTGAATGGCTGTTTGGTGCCGCCATAAGATTCTACAAGATGGCTAAGAAGTTTCCAACTCTCCGCAGAGATCTCACTTCAAATCTAGACTACTTGGAACAACGGATCGCACTGATAAGCCGCACCAGAATCATTTGGGTTTCTGAAAATTTAACAAGAACAATGGAGGATGGCAAGGAGCTGGCCAGAAGGTGCGACGAGGGGAATCTGAACTTCTTTGAAAAGGCTAAATATGCTAAAAAAATTGTTAAATGGGACGAATCTCGTCAAAGAATCTTGAAGTTAGTGCAAACTGATGCAAATCTTGTGGCTCAAAATCAAAACCAAGACCTTGTTACAACTTGGTGCGCAGTTCCCGACCTTCCAAGCAATACACTAAAACTGGATGCGCCTTTGGCGGAGATGAAGATGAAACTCCTTAGAGATGACGCGCTGTCAATGCTTGTGCTCACTGCTCCGGGAGGATGTGGGAAAACCACTTTGGCAAATATGTTCTGTCATGATCCACAAGTCAAAGATAAATTCAAGGACAATATCTTCTTTGCCACGGTTTCCAATAAGCCCAGCCACCTTGTTGTCCAAGAATTGTGTCAACATGCCCGATTCAAGGTACCTGCTTTAGAAAATGAAGAAATTGCATTTATCTGGCTGCCGCAATTTTTGAGGGAAGCAGGGCAAAATCCCTTACTGTTAATCTTGGATGATGTTCCGTCTGCATCAGAATCCCTTCTTGACAAGTTTGATGAAATCAAAATTCCAGATTACAAGATTTTGGTGACATCAAGATATCATTTTCCGAAATTCGGGCCTCCATATAATTTACAAGCATTGACAGATAAAGATGCACTGACTCTTTTTCGTCGTTCAGCATTTCTGCCAAATACGATCTGTAACATACCAGATGATCTTCAGATTCAGATACTAAACCACTGTAAGAGATTTCCGCTTGCCATTACAACCATAGGGACGTCACTTCGCAATCAGCCTGTAGAGAAATGGACAAGCAAACTCACAGAATTATCTAAAGGTTCTTCTATTTTTGATTCAGAGAAAATTTTGCTTGCGCTCCTCAAAAGTTGCTTAGATGACTTGAATAAAGAAATGGCTCCTGTCAAGGATTGTTTCATAGACCTTGCTTTATTTCCCCAAGACCAAAGAATTCCCGTTGCTTTCCTTCTTGATATGTGGGCAGAGTTATATGAGGGATCAAATGATGACGATTCAATAGTGAACCTATACCACCTCACCTTCCGCAATTTGGCCAGTCTTGTAGTCACAAGGACTAAGGATTTGGATGGATACTATGGTGAACATTTTGTTATCCAACATGATATGCTTAAATTGCTATCAATCCGTGCGAGTCATGATGAAGACCCAACAGGACATAGACTGATTGTAGACATACGTGGAGATGAGCTTCCACCATGGTGGACAGAGAAGAAAACAATGAAGGGTCGCTTAGTATCTGTCTTAACACCATGGTGGACAGAGAAGAAGCAGAAAACAAAGAAGGCTCGCTTAGTATCTGTCATAACTGGTGAATCTTCTTCAACAGAGTGGCACAACATGGATCTACCAAAAGGCGAGGTTCTAGTTTTGAATTTTCAAGCAAAGAACTATGCCTTACCCAAGTTCATGAAGAAAATGTGCAAGCTGAAGGTTCTAATAGTCACAAATGATGGCTTCTCACCTGCTGAGCTAAGCGACTTTGAACTGCTGTGTTCTTTGTCAAATCTAAAGAGACTAAGATTAGAGCATATTTCGATTCCTTTGATACGCGAGAACATCATTCAATCGAAGAGTCTAAAGAAGATATCTTTATTCATGTGTAACGTCAGTCAAGCATTTGGAAACTCTTCCATCCAAATTTTTGAGACATTCCCATACCTAGAGGAGTTGCACATCGACTATTGTAACGATTTGGTGGAATTGCCTGCCAAGCTATGTGATCTTATCCACCTAAAAGTTCTCAGCATCACCAACTCTCATAAGCTATCTGTCTTGCCTGAAGACATCGAAAAGCTGGCGAATTTAGAAGTGTTGAGGCTAAGGTCCTGCGCAGAATTAAAAAAGCTTCCAGGCTCGATTGAGAGACTCAATAAGTTGTGCTTTCTTGACATATATAATTGTTCCAGCATTAAGGACTTGCCCGAATGCATTGATAAGATGAATGGTTTAAGAAAAATCAACGTGGCACTATGTTCAAGATTGAAAGAGCTGCCTGTGTCAGTGTTTGATCTTGGTCAGCAGTTAGAGAAAGTGATATGTGACGAAGACGCAAGAAATTTATGGGAATCTGTCTTATCAAATCCCGACAAAATAAGGGTGGCTAAAGAAGAATTCAACCTGAATTGGCTCCACAGACTTCAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

807

Amino Acids

92.28

Weight (kDa)

7.73

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 146 - 304 4.9e-07 NB-ARC domain
LRR_14 PF23598 636 - 749 2.4e-14 Leucine-rich repeat region
LRR_13 PF23286 690 - 764 1.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1744
AatII GACGTC 1 cut(s) 1016
Acc16I TGCGCA 2 cut(s) 394, 2104
Acc36I ACCTGC 2 cut(s) 667, 1744
Acc65I GGTACC 1 cut(s) 655
AccB1I GGYRCC 2 cut(s) 62, 655
AccI GTMKAC 1 cut(s) 1443
AccII CGCG 2 cut(s) 476, 1824
AciI CCGC 7 cut(s) 66, 109, 166, 443, 698, 989, 1297
AclWI GGATC 4 cut(s) 158, 542, 1258, 1623
AcoI YGGCCR 2 cut(s) 228, 1305
AcuI CTGAAG 4 cut(s) 938, 1721, 2073, 2396
AcyI GRCGYC 1 cut(s) 1013
AfaI GTAC 1 cut(s) 657
AfiI CCNNNNNNNGG 3 cut(s) 652, 848, 1303
AhdI GACNNNNNGTC 1 cut(s) 478
AjnI CCWGG 1 cut(s) 2122
AjuI GAANNNNNNNTTGG 4 cut(s) 42, 74, 509, 541
AleI CACNNNNGTG 2 cut(s) 1471, 1525
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 3 cut(s) 1780, 1913, 2131
AlwI GGATC 4 cut(s) 158, 542, 1258, 1623
AoxI GGCC 3 cut(s) 228, 851, 1305
ApeKI GCWGC 3 cut(s) 26, 694, 2263
ArsI GACNNNNNNTTYG 3 cut(s) 1318, 1350, 2400
Asp700I GAANNNNTTC 2 cut(s) 771, 1900
Asp718I GGTACC 1 cut(s) 655
AspLEI GCGC 5 cut(s) 395, 436, 478, 1111, 2105
AspS9I GGNCC 2 cut(s) 851, 2097
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 6 cut(s) 832, 1282, 1364, 1595, 1725, 2014
AvaII GGWCC 1 cut(s) 2097
BaeI ACNNNNGTAYC 2 cut(s) 1336, 1369
BalI TGGCCA 2 cut(s) 230, 1307
BanI GGYRCC 2 cut(s) 62, 655
BbsI GAAGAC 3 cut(s) 1422, 2061, 2325
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 3 cut(s) 38, 681, 2250
BccI CCATC 5 cut(s) 79, 209, 1334, 1718, 1913
BciT130I CCWGG 1 cut(s) 2124
BciVI GTATCC 1 cut(s) 1337
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 3 cut(s) 1780, 1913, 2131
BfaI CTAG 3 cut(s) 131, 1637, 1934
BfmI CTRYAG 2 cut(s) 21, 1032
BfuAI ACCTGC 2 cut(s) 667, 1744
BfuI GTATCC 1 cut(s) 1337
BglII AGATCT 1 cut(s) 115
BisI GCNGC 6 cut(s) 27, 66, 166, 695, 698, 2264
BlpI GCTNAGC 2 cut(s) 1740, 1745
BlsI GCNGC 6 cut(s) 28, 67, 167, 696, 699, 2265
Bme1390I CCNGG 2 cut(s) 504, 2124
Bme18I GGWCC 1 cut(s) 2097
BmeRI GACNNNNNGTC 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 851, 2097
BmiI GGNNCC 4 cut(s) 64, 657, 1152, 2402
BmrFI CCNGG 2 cut(s) 504, 2124
BmsI GCATC 5 cut(s) 331, 421, 770, 877, 2028
BpiI GAAGAC 3 cut(s) 1422, 2061, 2325
Bpu10I CCTNAGC 1 cut(s) 2093
Bpu1102I GCTNAGC 2 cut(s) 1740, 1745
BpuMI CCSGG 1 cut(s) 504
BsaAI YACGTR 1 cut(s) 1451
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 1013
BsaJI CCNNGG 3 cut(s) 591, 1469, 1523
Bsc4I CCNNNNNNNGG 3 cut(s) 652, 848, 1303
Bse1I ACTGG 3 cut(s) 432, 1308, 1585
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 1 cut(s) 2124
BseDI CCNNGG 3 cut(s) 591, 1469, 1523
BseGI GGATG 6 cut(s) 220, 436, 515, 751, 1345, 1905
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 652, 848, 1303
BseMII CTCAG 2 cut(s) 1731, 2029
BseNI ACTGG 3 cut(s) 432, 1308, 1585
BseRI GAGGAG 2 cut(s) 1103, 1952
BseXI GCAGC 3 cut(s) 38, 681, 2250
BseYI CCCAGC 1 cut(s) 608
Bsh1236I CGCG 2 cut(s) 476, 1824
BshFI GGCC 3 cut(s) 230, 853, 1307
BshNI GGYRCC 2 cut(s) 62, 655
BsiHKAI GWGCWC 1 cut(s) 495
BsiSI CCGG 1 cut(s) 503
BslFI GGGAC 2 cut(s) 314, 1024
BslI CCNNNNNNNGG 3 cut(s) 652, 848, 1303
BsmAI GTCTC 3 cut(s) 1780, 1913, 2131
BsmFI GGGAC 2 cut(s) 314, 1024
BsmI GAATGC 1 cut(s) 2204
BsnI GGCC 3 cut(s) 230, 853, 1307
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 9 cut(s) 115, 150, 547, 930, 949, 1250, 1615, 1993, 2281
Bsp1720I GCTNAGC 2 cut(s) 1740, 1745
Bsp19I CCATGG 2 cut(s) 1469, 1523
BspACI CCGC 7 cut(s) 66, 109, 166, 443, 698, 989, 1297
BspANI GGCC 3 cut(s) 230, 853, 1307
BspCNI CTCAG 2 cut(s) 1732, 2028
BspFNI CGCG 2 cut(s) 476, 1824
BspHI TCATGA 3 cut(s) 544, 1408, 1680
BspLI GGNNCC 4 cut(s) 64, 657, 1152, 2402
BspMI ACCTGC 2 cut(s) 667, 1744
BspPI GGATC 4 cut(s) 158, 542, 1258, 1623
BspT107I GGYRCC 2 cut(s) 62, 655
BsrI ACTGG 3 cut(s) 432, 1308, 1585
BssECI CCNNGG 3 cut(s) 591, 1469, 1523
BssMI GATC 9 cut(s) 115, 150, 547, 930, 949, 1250, 1615, 1993, 2281
BssNI GRCGYC 1 cut(s) 1013
BssT1I CCWWGG 2 cut(s) 1469, 1523
Bst2UI CCWGG 1 cut(s) 2124
Bst4CI ACNGT 3 cut(s) 595, 735, 977
Bst6I CTCTTC 2 cut(s) 1838, 1906
BstACI GRCGYC 1 cut(s) 1013
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 1451
BstC8I GCNNGC 7 cut(s) 228, 993, 1107, 1561, 1697, 1980, 2070
BstDSI CCRYGG 3 cut(s) 591, 1469, 1523
BstF5I GGATG 6 cut(s) 220, 436, 515, 751, 1345, 1905
BstFNI CGCG 2 cut(s) 476, 1824
BstHHI GCGC 5 cut(s) 395, 436, 478, 1111, 2105
BstKTI GATC 9 cut(s) 118, 153, 550, 933, 952, 1253, 1618, 1996, 2284
BstMAI GTCTC 3 cut(s) 1780, 1913, 2131
BstMBI GATC 9 cut(s) 115, 150, 547, 930, 949, 1250, 1615, 1993, 2281
BstMWI GCNNNNNNNGC 7 cut(s) 11, 26, 278, 440, 484, 1387, 1984
BstNI CCWGG 1 cut(s) 2124
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 2 cut(s) 502, 2122
BstSFI CTRYAG 2 cut(s) 21, 1032
BstUI CGCG 2 cut(s) 476, 1824
BstV1I GCAGC 3 cut(s) 38, 681, 2250
BstV2I GAAGAC 3 cut(s) 1422, 2061, 2325
BstX2I RGATCY 2 cut(s) 115, 1615
BstXI CCANNNNNNTGG 2 cut(s) 51, 1473
BstYI RGATCY 2 cut(s) 115, 1615
BsuI GTATCC 1 cut(s) 1337
BsuRI GGCC 3 cut(s) 230, 853, 1307
BtgI CCRYGG 3 cut(s) 591, 1469, 1523
BtsCI GGATG 6 cut(s) 220, 436, 515, 751, 1345, 1905
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 5 cut(s) 155, 495, 890, 973, 2280
BveI ACCTGC 2 cut(s) 667, 1744
Cac8I GCNNGC 7 cut(s) 228, 993, 1107, 1561, 1697, 1980, 2070
CciI TCATGA 3 cut(s) 544, 1408, 1680
CfoI GCGC 5 cut(s) 395, 436, 478, 1111, 2105
Cfr13I GGNCC 2 cut(s) 851, 2097
CseI GACGC 3 cut(s) 3, 482, 2330
Csp6I GTAC 1 cut(s) 656
CspCI CAANNNNNGTGG 2 cut(s) 333, 368
CviAII CATG 9 cut(s) 545, 641, 1373, 1409, 1470, 1524, 1612, 1681, 1870
CviQI GTAC 1 cut(s) 656
DpnI GATC 9 cut(s) 117, 152, 549, 932, 951, 1252, 1617, 1995, 2283
DpnII GATC 9 cut(s) 115, 150, 547, 930, 949, 1250, 1615, 1993, 2281
DriI GACNNNNNGTC 1 cut(s) 478
EaeI YGGCCR 2 cut(s) 228, 1305
Eam1104I CTCTTC 2 cut(s) 1838, 1906
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 2 cut(s) 1838, 1906
EciI GGCGGA 1 cut(s) 458
Eco130I CCWWGG 2 cut(s) 1469, 1523
Eco32I GATATC 2 cut(s) 833, 1860
Eco47I GGWCC 1 cut(s) 2097
Eco57I CTGAAG 4 cut(s) 938, 1721, 2073, 2396
EcoO109I RGGNCCY 1 cut(s) 2097
EcoRI GAATTC 2 cut(s) 1203, 2384
EcoRII CCWGG 1 cut(s) 2122
EcoRV GATATC 2 cut(s) 833, 1860
EcoT14I CCWWGG 2 cut(s) 1469, 1523
EcoT22I ATGCAT 1 cut(s) 2204
ErhI CCWWGG 2 cut(s) 1469, 1523
FaeI CATG 9 cut(s) 548, 644, 1376, 1412, 1473, 1527, 1615, 1684, 1873
FalI AAGNNNNNCTT 6 cut(s) 1487, 1519, 1547, 1579, 2138, 2170
FaqI GGGAC 2 cut(s) 314, 1024
FatI CATG 9 cut(s) 544, 640, 1372, 1408, 1469, 1523, 1611, 1680, 1869
FblI GTMKAC 1 cut(s) 1443
Fnu4HI GCNGC 6 cut(s) 27, 66, 166, 695, 698, 2264
FokI GGATG 6 cut(s) 227, 443, 522, 758, 1352, 1892
Fsp4HI GCNGC 6 cut(s) 27, 66, 166, 695, 698, 2264
FspBI CTAG 3 cut(s) 131, 1637, 1934
FspI TGCGCA 2 cut(s) 394, 2104
GlaI GCGC 5 cut(s) 394, 435, 477, 1110, 2104
GluI GCNGC 6 cut(s) 27, 66, 166, 695, 698, 2264
GsaI CCCAGC 1 cut(s) 612
HaeIII GGCC 3 cut(s) 230, 853, 1307
HapII CCGG 1 cut(s) 503
HgaI GACGC 3 cut(s) 3, 482, 2330
HhaI GCGC 5 cut(s) 395, 436, 478, 1111, 2105
Hin1I GRCGYC 1 cut(s) 1013
Hin1II CATG 9 cut(s) 548, 644, 1376, 1412, 1473, 1527, 1615, 1684, 1873
Hin6I GCGC 5 cut(s) 393, 434, 476, 1109, 2103
HinP1I GCGC 5 cut(s) 393, 434, 476, 1109, 2103
HincII GTYRAC 1 cut(s) 638
HindII GTYRAC 1 cut(s) 638
HindIII AAGCTT 1 cut(s) 2117
HpaII CCGG 1 cut(s) 503
HphI GGTGA 6 cut(s) 832, 1282, 1364, 1595, 1725, 2014
Hpy166II GTNNAC 6 cut(s) 638, 1276, 1355, 1444, 1476, 1530
Hpy188I TCNGA 8 cut(s) 190, 255, 766, 843, 957, 963, 1093, 2415
Hpy8I GTNNAC 6 cut(s) 638, 1276, 1355, 1444, 1476, 1530
Hpy99I CGWCG 2 cut(s) 245, 909
HpyAV CCTTC 8 cut(s) 228, 416, 782, 1229, 1303, 1489, 1549, 1696
HpyCH4III ACNGT 3 cut(s) 595, 735, 977
HpyCH4IV ACGT 4 cut(s) 1013, 1450, 1878, 2235
HpyCH4V TGCA 8 cut(s) 334, 344, 683, 761, 890, 1695, 1945, 2202
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 26, 278, 440, 484, 1387, 1984
HpySE526I ACGT 4 cut(s) 1013, 1450, 1878, 2235
Hsp92I GRCGYC 1 cut(s) 1013
Hsp92II CATG 9 cut(s) 548, 644, 1376, 1412, 1473, 1527, 1615, 1684, 1873
HspAI GCGC 5 cut(s) 393, 434, 476, 1109, 2103
KpnI GGTACC 1 cut(s) 659
Kzo9I GATC 9 cut(s) 115, 150, 547, 930, 949, 1250, 1615, 1993, 2281
LmnI GCTCC 5 cut(s) 223, 505, 1116, 1156, 2406
Lsp1109I GCAGC 3 cut(s) 38, 681, 2250
LweI GCATC 5 cut(s) 331, 421, 770, 877, 2028
MaeI CTAG 3 cut(s) 131, 1637, 1934
MaeII ACGT 4 cut(s) 1013, 1450, 1878, 2235
MaeIII GTNAC 9 cut(s) 379, 820, 935, 1014, 1318, 1714, 1874, 1958, 2312
MalI GATC 9 cut(s) 117, 152, 549, 932, 951, 1252, 1617, 1995, 2283
MboI GATC 9 cut(s) 115, 150, 547, 930, 949, 1250, 1615, 1993, 2281
MflI RGATCY 2 cut(s) 115, 1615
MhlI GDGCHC 1 cut(s) 495
MlsI TGGCCA 2 cut(s) 230, 1307
MluNI TGGCCA 2 cut(s) 230, 1307
MlyI GAGTC 4 cut(s) 889, 1414, 1855, 2133
MmeI TCCRAC 2 cut(s) 125, 1393
Mox20I TGGCCA 2 cut(s) 230, 1307
Mph1103I ATGCAT 1 cut(s) 2204
MroXI GAANNNNTTC 2 cut(s) 771, 1900
MscI TGGCCA 2 cut(s) 230, 1307
MseI TTAA 8 cut(s) 197, 294, 737, 1383, 1517, 2111, 2184, 2222
MslI CAYNNNNRTG 3 cut(s) 49, 1471, 1525
Msp20I TGGCCA 2 cut(s) 230, 1307
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 2 cut(s) 504, 2124
Mva1269I GAATGC 1 cut(s) 2204
MvaI CCWGG 1 cut(s) 2124
MvnI CGCG 2 cut(s) 476, 1824
MwoI GCNNNNNNNGC 7 cut(s) 11, 26, 278, 440, 484, 1387, 1984
NciI CCSGG 1 cut(s) 504
NcoI CCATGG 2 cut(s) 1469, 1523
NdeII GATC 9 cut(s) 115, 150, 547, 930, 949, 1250, 1615, 1993, 2281
NlaIII CATG 9 cut(s) 548, 644, 1376, 1412, 1473, 1527, 1615, 1684, 1873
NlaIV GGNNCC 4 cut(s) 64, 657, 1152, 2402
NmuCI GTSAC 5 cut(s) 820, 1014, 1318, 1714, 2312
NsbI TGCGCA 2 cut(s) 394, 2104
NsiI ATGCAT 1 cut(s) 2204
NspI RCATGY 1 cut(s) 644
OliI CACNNNNGTG 2 cut(s) 1471, 1525
PagI TCATGA 3 cut(s) 544, 1408, 1680
PaqCI CACCTGC 1 cut(s) 1744
PctI GAATGC 1 cut(s) 2204
PdmI GAANNNNTTC 2 cut(s) 771, 1900
PfoI TCCNGGA 1 cut(s) 502
PkrI GCNGC 6 cut(s) 28, 67, 167, 696, 699, 2265
PleI GAGTC 4 cut(s) 889, 1413, 1854, 2133
PpsI GAGTC 4 cut(s) 889, 1413, 1854, 2133
Ppu21I YACGTR 1 cut(s) 1451
PpuMI RGGWCCY 1 cut(s) 2097
Psp5II RGGWCCY 1 cut(s) 2097
Psp6I CCWGG 1 cut(s) 2122
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 1 cut(s) 2122
PspN4I GGNNCC 4 cut(s) 64, 657, 1152, 2402
PspPI GGNCC 2 cut(s) 851, 2097
PspPPI RGGWCCY 1 cut(s) 2097
PsuI RGATCY 2 cut(s) 115, 1615
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 1 cut(s) 657
RsaNI GTAC 1 cut(s) 656
RseI CAYNNNNRTG 3 cut(s) 49, 1471, 1525
SaqAI TTAA 8 cut(s) 197, 294, 737, 1383, 1517, 2111, 2184, 2222
SatI GCNGC 6 cut(s) 27, 66, 166, 695, 698, 2264
Sau3AI GATC 9 cut(s) 115, 150, 547, 930, 949, 1250, 1615, 1993, 2281
Sau96I GGNCC 2 cut(s) 851, 2097
SchI GAGTC 4 cut(s) 889, 1414, 1855, 2133
ScrFI CCNGG 2 cut(s) 504, 2124
SduI GDGCHC 1 cut(s) 495
SfaNI GCATC 5 cut(s) 331, 421, 770, 877, 2028
SfcI CTRYAG 2 cut(s) 21, 1032
SinI GGWCC 1 cut(s) 2097
SmiMI CAYNNNNRTG 3 cut(s) 49, 1471, 1525
SsiI CCGC 7 cut(s) 66, 109, 166, 443, 698, 989, 1297
SspMI CTAG 3 cut(s) 131, 1637, 1934
StyD4I CCNGG 2 cut(s) 502, 2122
StyI CCWWGG 2 cut(s) 1469, 1523
TaaI ACNGT 3 cut(s) 595, 735, 977
TaiI ACGT 4 cut(s) 1016, 1453, 1881, 2238
TaqI TCGA 5 cut(s) 1808, 1841, 1950, 2061, 2129
TauI GCSGC 3 cut(s) 68, 168, 700
Tru1I TTAA 8 cut(s) 197, 294, 737, 1383, 1517, 2111, 2184, 2222
Tru9I TTAA 8 cut(s) 197, 294, 737, 1383, 1517, 2111, 2184, 2222
TscAI CASTG 5 cut(s) 162, 502, 897, 980, 2280
TseFI GTSAC 5 cut(s) 820, 1014, 1318, 1714, 2312
TseI GCWGC 3 cut(s) 26, 694, 2263
Tsp45I GTSAC 5 cut(s) 820, 1014, 1318, 1714, 2312
TspGWI ACGGA 3 cut(s) 163, 744, 1388
TspRI CASTG 5 cut(s) 162, 502, 897, 980, 2280
VpaK11BI GGWCC 1 cut(s) 2097
XbaI TCTAGA 1 cut(s) 130
XceI RCATGY 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 1443
XmnI GAANNNNTTC 2 cut(s) 771, 1900
XspI CTAG 3 cut(s) 131, 1637, 1934
ZraI GACGTC 1 cut(s) 1014
Zsp2I ATGCAT 1 cut(s) 2204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.