Rh2DG555700

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000039
Physical Location & Seq
Forward (+)
78580172 .. 78583416
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG555700.1

Sequence Viewer

Length: 2514 bp
ATGGATATTAATCCGGTGGAGTTTTTCGGAGGTGCTGTTCTAGGAACAGCGTTCAGTGCAGTCTATGCTGGCGTTGAAGATCTGATTGTCAAGAGCCACTTGTTCAGAAATCTCTTCAAAAACATCAAATCCAGGCTAGACACTCTGAAGTCACTCATGGCCGAGATGGCTCGGTCTAACCAGCAACTGTCTTACACACAGAACGATGAGCTTAGAAGTTTAGAAGAAGTTCTAAAGGTCGGCGAAGAGCTTGTTCGAAAGTGCTCAAATGTTAGGCGGTGGGATCTCTACAGGAAGTACAAATATGCCACAAAACTTCTTGCATGGGAAGAAGATCTTGGAAAACAATTGCAGCTGCTGCAGGTGTACGGAATCAGGGATGGGAAGAGGACCGCGGTGGCAGTAGAGAATATAGAGGTTTCTGTAGGGAGTGTGCAGGTGGGTGTCAACCAAATTTCTAGTACTCTCGAGGAGGTGAACCAACTCATGAAAGAGAATTTTGTGATACAAGACCAATCTGCTGAAAGTCAAGCTTGGTGTGCAGTCCCTGAACTTCCAGAGGTTACAGTTGGATTGAAAGCTCCTTTGAAGGATTTGAAGACACAGCTTCTTGGGAATGATGGATTTTCAATGCTTGTGCTCACCGCTCCCGGAGGATGTGGGAAAACTACCTTGGCAACAAAATTTTGCAAAGATCAGGAAGTCAAAGATAAATTCGGCAAAAATATCTTCTTTGTTACTGTTTCGAAGAAGTATAGCTTAGATTCTATTGTAGAACAACTATATCGACACAAGGGTTCTCAAGTTCCTGCTTTCCAAAATGAAGTGAATGCCGTTCAGTGGCTGCAAACATTTTTGAAGGAAGAAGGAGAGAAACCTTCACTGTTGGTCTTGGATGATGTTTGGTCTGGATCAGAATCTCTTCTTGACAAATTTGAATGCAGAATGGCAAATATTAAGATTCTTGTGACATCAAGATCTGAATTTCCCGCGTTCAGTCCTCTATATCGTTTAAAAATGTTGAATAATGACGATGCAATGGAACTGTTTCGTCATTCAGCATCATTGAATGATAAGAGCTCTCATATTCCAGCACATCTTTCAAAAAAGATAGTTGAGCGTTGTAAGGGATTTCCACTTGCCATTAAAGTGGTGGGCAGATCGCTTCGTGCACAACCTCTAGAGATGTGGCAAAACAGACTAAGTCAATGGGCTAAAGGTTCATCTATTTTTGATTTTGAGACTGATTTACTTCTTTCCCTCCAAAGCAGCTTGGATGCTTTGGATAAGGAAAGGGCTATCATCAGAGAATGTTTCTTGGACCTTGGTTCATTTCCTGAAGACAAACAAATCCCCGTTGATGCCCTCATTGATATATGGTCCGAGTTATATGATATTGATGAAGACACTTCATGTATTGCAAACCTCTATGAACTCAACCATCGGAGTCTTGCTAATCTTATAGACAGAAGGGCATCGAGCTTGGATGGGGAAGGATATTATGGTTACCACTATGTCACCCAACATGATGTGCTTAGGGAGCTCGCTATTTATCACTCGAAACTAGATCCGATAGAGCATAGAGAAAGATTGATCATAGACATATGTGGAAGCAATCTTCCCAAGTGGTGGAGGGAACAAAAGTATCAAGTTATGAAAGCTCGCCTTCTATCTATATCAACTGATGGAGAGTCTTCAACTTCGAAATGGCCCAGCATGCATCTGCCAGAAACAGAGGTACTGCTCTTGAATTTTCGGGAGCCAGAGCATGTTATACCCGAATTTGTGGAGAAAATGGACAAATTGAAGGTACTAGTAATCACAAGTGAGGATTTCCGGGGTAGAGCTCTCTTGCATAATTTTCAAGTACTAAGTCGGTTATCCAATCTAAAGAGAATCAGATTAAGGGGCATCTCAATTTCATCCATATTCAAGAACTCCATACAAGTGAAGAGTCTACGGAAGTTGTCTTTGATCAGATTTGATACCAAAATCGGTTCAGCCAAATTTTCCCATGCTTTTCCAAATCTAGAAGAGATACACATCAAAAACGCTGCAATGTTTTTGCACAGTGATACAGGGACAGTACTTCGCGGGCTATGTGATCTGATTTCCCTAAAGAAGGTCACCCTCACAGGTCCAGGACTACGAGTATCGGCTTTGCCTGATGAGATTGGAAATTTGGTCAATTTACAAGTATTGATGCTGAGGTCATTGGGTCATCTTTCAAAGCTTCCAAACTCGATCAAAAACCTCAAGAAGTTGAACTTTCTTGGCATCTTTAATTGCTGGGACCTCTGCGAGTTTCCTGAATGCATTGGTGAAATGAGGAGTTTAAGAAAGATCGACTTGAGACTTTGCCGGTCATTGAAGAAGCTGCCTGGATCAGTATTGGATTTGGAGCAGTTAGAGGAGGTGATATGTGATAAACATACAGAACGCTTATGGGAATCCCTGTTGCCCAGTTTCAGAGACAGAAACCAAGACATACGCATAGTGATTGGGCCGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

837

Amino Acids

95.35

Weight (kDa)

7.81

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 137 3.1e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 195 - 354 1.5e-17 NB-ARC domain
AAA_22 PF13401 210 - 301 3.2e-06 AAA domain
LRR_14 PF23598 694 - 805 4.6e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 352, 427
Acc36I ACCTGC 2 cut(s) 352, 427
AccB7I CCANNNNNTGG 1 cut(s) 1629
AccBSI CCGCTC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 1957
AccII CGCG 3 cut(s) 395, 992, 2094
AciI CCGC 7 cut(s) 277, 393, 395, 645, 990, 2094, 2507
AclWI GGATC 4 cut(s) 291, 919, 1562, 2392
AcoI YGGCCR 1 cut(s) 159
AcuI CTGAAG 2 cut(s) 167, 1359
AfaI GTAC 7 cut(s) 299, 368, 463, 1740, 1812, 1869, 2088
AfiI CCNNNNNNNGG 3 cut(s) 840, 1629, 2122
AhlI ACTAGT 1 cut(s) 1813
AjnI CCWGG 3 cut(s) 131, 2140, 2380
AjuI GAANNNNNNNTTGG 4 cut(s) 321, 353, 656, 688
AloI GAACNNNNNNTCC 2 cut(s) 21, 53
Alw21I GWGCWC 6 cut(s) 266, 642, 1082, 1174, 1545, 1849
Alw26I GTCTC 3 cut(s) 1235, 2347, 2466
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 4 cut(s) 291, 919, 1562, 2392
AlwNI CAGNNNCTG 4 cut(s) 187, 358, 548, 844
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 3 cut(s) 159, 1709, 2504
ApaLI GTGCAC 1 cut(s) 1170
ApeKI GCWGC 7 cut(s) 352, 355, 358, 844, 1269, 2054, 2377
AseI ATTAAT 1 cut(s) 9
Asp700I GAANNNNTTC 3 cut(s) 228, 921, 1047
AspS9I GGNCC 7 cut(s) 390, 1321, 1380, 1710, 2138, 2293, 2504
AsuC2I CCSGG 2 cut(s) 651, 1838
AsuHPI GGTGA 6 cut(s) 487, 634, 1510, 2119, 2333, 2428
AsuII TTCGAA 3 cut(s) 256, 746, 1703
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 5 cut(s) 390, 1321, 1380, 2138, 2293
BaeGI GKGCMC 1 cut(s) 1174
BaeI ACNNNNGTAYC 2 cut(s) 1628, 1661
BanII GRGCYC 3 cut(s) 1082, 1545, 1849
BbsI GAAGAC 4 cut(s) 605, 1347, 1410, 1686
Bbv12I GWGCWC 6 cut(s) 266, 642, 1082, 1174, 1545, 1849
BbvCI CCTCAGC 1 cut(s) 2207
BbvI GCAGC 7 cut(s) 342, 345, 364, 831, 1281, 2041, 2364
BccI CCATC 6 cut(s) 160, 374, 614, 1449, 1481, 1679
BceAI ACGGC 1 cut(s) 818
BciT130I CCWGG 3 cut(s) 133, 2142, 2382
BclI TGATCA 2 cut(s) 1593, 1974
BcnI CCSGG 2 cut(s) 651, 1838
BcoDI GTCTC 3 cut(s) 1235, 2347, 2466
BcuI ACTAGT 1 cut(s) 1813
BfaI CTAG 7 cut(s) 41, 137, 459, 1181, 1565, 1814, 2030
BfmI CTRYAG 3 cut(s) 289, 359, 423
BfuAI ACCTGC 2 cut(s) 352, 427
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 3 cut(s) 79, 334, 977
BisI GCNGC 8 cut(s) 353, 356, 359, 845, 1270, 2055, 2378, 2507
BlsI GCNGC 8 cut(s) 354, 357, 360, 846, 1271, 2056, 2379, 2508
BmcAI AGTACT 3 cut(s) 463, 1869, 2088
Bme1390I CCNGG 5 cut(s) 133, 651, 1838, 2142, 2382
Bme18I GGWCC 5 cut(s) 390, 1321, 1380, 2138, 2293
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 7 cut(s) 390, 1321, 1380, 1710, 2138, 2293, 2504
BmiI GGNNCC 2 cut(s) 1761, 2294
BmrFI CCNGG 5 cut(s) 133, 651, 1838, 2142, 2382
BmrI ACTGGG 1 cut(s) 2457
BmsI GCATC 9 cut(s) 1024, 1070, 1267, 1351, 1484, 1729, 1920, 2193, 2286
BmuI ACTGGG 1 cut(s) 2457
BpiI GAAGAC 4 cut(s) 605, 1347, 1410, 1686
Bpu10I CCTNAGC 2 cut(s) 1535, 2207
Bpu14I TTCGAA 3 cut(s) 256, 746, 1703
BpuEI CTTGAG 3 cut(s) 786, 2240, 2371
BpuMI CCSGG 2 cut(s) 651, 1838
BsaBI GATNNNNATC 3 cut(s) 9, 916, 2042
BsaJI CCNNGG 4 cut(s) 393, 672, 1324, 1837
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 3 cut(s) 840, 1629, 2122
Bse118I RCCGGY 1 cut(s) 2361
Bse1I ACTGG 1 cut(s) 2463
Bse3DI GCAATG 2 cut(s) 1044, 2064
Bse8I GATNNNNATC 3 cut(s) 9, 916, 2042
BseBI CCWGG 3 cut(s) 133, 2142, 2382
BseDI CCNNGG 4 cut(s) 393, 672, 1324, 1837
BseGI GGATG 6 cut(s) 385, 662, 901, 1282, 1492, 1922
BseJI GATNNNNATC 3 cut(s) 9, 916, 2042
BseLI CCNNNNNNNGG 3 cut(s) 840, 1629, 2122
BseMI GCAATG 2 cut(s) 1044, 2064
BseMII CTCAG 1 cut(s) 2198
BseNI ACTGG 1 cut(s) 2463
BseRI GAGGAG 3 cut(s) 485, 2344, 2426
BseSI GKGCMC 1 cut(s) 1174
BseXI GCAGC 7 cut(s) 342, 345, 364, 831, 1281, 2041, 2364
BseYI CCCAGC 2 cut(s) 1712, 2289
BsgI GTGCAG 3 cut(s) 78, 455, 561
Bsh1236I CGCG 3 cut(s) 395, 992, 2094
BshFI GGCC 3 cut(s) 161, 1711, 2506
BsiHKAI GWGCWC 6 cut(s) 266, 642, 1082, 1174, 1545, 1849
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 4 cut(s) 14, 651, 1837, 2362
BslFI GGGAC 3 cut(s) 530, 2095, 2306
BslI CCNNNNNNNGG 3 cut(s) 840, 1629, 2122
BsmAI GTCTC 3 cut(s) 1235, 2347, 2466
BsmFI GGGAC 3 cut(s) 530, 2095, 2306
BsmI GAATGC 3 cut(s) 835, 944, 2318
BsnI GGCC 3 cut(s) 161, 1711, 2506
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 3 cut(s) 256, 746, 1703
Bsp1286I GDGCHC 6 cut(s) 266, 642, 1082, 1174, 1545, 1849
BspACI CCGC 7 cut(s) 277, 393, 395, 645, 990, 2094, 2507
BspANI GGCC 3 cut(s) 161, 1711, 2506
BspCNI CTCAG 1 cut(s) 2199
BspFNI CGCG 3 cut(s) 395, 992, 2094
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 2 cut(s) 1761, 2294
BspMAI CTGCAG 1 cut(s) 363
BspMI ACCTGC 2 cut(s) 352, 427
BspPI GGATC 4 cut(s) 291, 919, 1562, 2392
BspQI GCTCTTC 1 cut(s) 240
BspT104I TTCGAA 3 cut(s) 256, 746, 1703
BsrBI CCGCTC 1 cut(s) 647
BsrDI GCAATG 2 cut(s) 1044, 2064
BsrFI RCCGGY 1 cut(s) 2361
BsrI ACTGG 1 cut(s) 2463
BssAI RCCGGY 1 cut(s) 2361
BssECI CCNNGG 4 cut(s) 393, 672, 1324, 1837
BssT1I CCWWGG 2 cut(s) 672, 1324
Bst2UI CCWGG 3 cut(s) 133, 2142, 2382
Bst4CI ACNGT 7 cut(s) 189, 568, 742, 885, 1047, 2072, 2086
Bst6I CTCTTC 6 cut(s) 119, 240, 380, 927, 1946, 2028
BstAPI GCANNNNNTGC 2 cut(s) 65, 358
BstBI TTCGAA 3 cut(s) 256, 746, 1703
BstC8I GCNNGC 5 cut(s) 70, 1545, 1663, 1718, 2096
BstDEI CTNAG 6 cut(s) 212, 760, 1202, 1535, 1871, 2207
BstDSI CCRYGG 1 cut(s) 393
BstEII GGTNACC 2 cut(s) 1505, 2125
BstENI CCTNNNNNAGG 1 cut(s) 2120
BstF5I GGATG 6 cut(s) 385, 662, 901, 1282, 1492, 1922
BstFNI CGCG 3 cut(s) 395, 992, 2094
BstMAI GTCTC 3 cut(s) 1235, 2347, 2466
BstMWI GCNNNNNNNGC 7 cut(s) 56, 65, 167, 358, 539, 1540, 1717
BstNI CCWGG 3 cut(s) 133, 2142, 2382
BstNSI RCATGY 2 cut(s) 1720, 1772
BstPI GGTNACC 2 cut(s) 1505, 2125
BstSCI CCNGG 5 cut(s) 131, 649, 1836, 2140, 2380
BstSFI CTRYAG 3 cut(s) 289, 359, 423
BstSLI GKGCMC 1 cut(s) 1174
BstUI CGCG 3 cut(s) 395, 992, 2094
BstV1I GCAGC 7 cut(s) 342, 345, 364, 831, 1281, 2041, 2364
BstV2I GAAGAC 4 cut(s) 605, 1347, 1410, 1686
BstX2I RGATCY 5 cut(s) 79, 283, 334, 977, 1567
BstXI CCANNNNNNTGG 1 cut(s) 1150
BstYI RGATCY 5 cut(s) 79, 283, 334, 977, 1567
BsuRI GGCC 3 cut(s) 161, 1711, 2506
BtgI CCRYGG 1 cut(s) 393
BtsCI GGATG 6 cut(s) 385, 662, 901, 1282, 1492, 1922
BtsIMutI CAGTG 4 cut(s) 61, 845, 881, 2077
BveI ACCTGC 2 cut(s) 352, 427
Cac8I GCNNGC 5 cut(s) 70, 1545, 1663, 1718, 2096
CaiI CAGNNNCTG 4 cut(s) 187, 358, 548, 844
CciI TCATGA 1 cut(s) 486
Cfr10I RCCGGY 1 cut(s) 2361
Cfr13I GGNCC 7 cut(s) 390, 1321, 1380, 1710, 2138, 2293, 2504
Cfr42I CCGCGG 1 cut(s) 396
Csp6I GTAC 7 cut(s) 298, 367, 462, 1739, 1811, 1868, 2087
CviAII CATG 9 cut(s) 157, 324, 487, 1413, 1526, 1717, 1769, 2015, 2511
CviQI GTAC 7 cut(s) 298, 367, 462, 1739, 1811, 1868, 2087
DdeI CTNAG 6 cut(s) 212, 760, 1202, 1535, 1871, 2207
DraI TTTAAA 1 cut(s) 1014
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 6 cut(s) 119, 240, 380, 927, 1946, 2028
EarI CTCTTC 6 cut(s) 119, 240, 380, 927, 1946, 2028
Ecl136II GAGCTC 3 cut(s) 1080, 1543, 1847
Eco130I CCWWGG 2 cut(s) 672, 1324
Eco24I GRGCYC 3 cut(s) 1082, 1545, 1849
Eco47I GGWCC 5 cut(s) 390, 1321, 1380, 2138, 2293
Eco53kI GAGCTC 3 cut(s) 1080, 1543, 1847
Eco57I CTGAAG 2 cut(s) 167, 1359
Eco88I CYCGRG 1 cut(s) 467
Eco91I GGTNACC 2 cut(s) 1505, 2125
EcoICRI GAGCTC 3 cut(s) 1080, 1543, 1847
EcoNI CCTNNNNNAGG 1 cut(s) 2120
EcoO109I RGGNCCY 1 cut(s) 2293
EcoO65I GGTNACC 2 cut(s) 1505, 2125
EcoRII CCWGG 3 cut(s) 131, 2140, 2380
EcoT14I CCWWGG 2 cut(s) 672, 1324
EcoT22I ATGCAT 2 cut(s) 1722, 2318
EcoT38I GRGCYC 3 cut(s) 1082, 1545, 1849
ErhI CCWWGG 2 cut(s) 672, 1324
FaeI CATG 9 cut(s) 160, 327, 490, 1416, 1529, 1720, 1772, 2018, 2514
FaqI GGGAC 3 cut(s) 530, 2095, 2306
FatI CATG 9 cut(s) 156, 323, 486, 1412, 1525, 1716, 1768, 2014, 2510
FauI CCCGC 2 cut(s) 997, 2087
FauNDI CATATG 1 cut(s) 1604
FbaI TGATCA 2 cut(s) 1593, 1974
FblI GTMKAC 1 cut(s) 1957
Fnu4HI GCNGC 8 cut(s) 353, 356, 359, 845, 1270, 2055, 2378, 2507
FokI GGATG 6 cut(s) 392, 669, 908, 1289, 1499, 1909
FriOI GRGCYC 3 cut(s) 1082, 1545, 1849
Fsp4HI GCNGC 8 cut(s) 353, 356, 359, 845, 1270, 2055, 2378, 2507
FspBI CTAG 7 cut(s) 41, 137, 459, 1181, 1565, 1814, 2030
GluI GCNGC 8 cut(s) 353, 356, 359, 845, 1270, 2055, 2378, 2507
GsaI CCCAGC 2 cut(s) 1716, 2293
HaeIII GGCC 3 cut(s) 161, 1711, 2506
HapII CCGG 4 cut(s) 14, 651, 1837, 2362
Hin1II CATG 9 cut(s) 160, 327, 490, 1416, 1529, 1720, 1772, 2018, 2514
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 2 cut(s) 531, 2231
HinfI GANTC 9 cut(s) 372, 764, 917, 961, 1447, 1691, 1896, 1954, 2450
HpaII CCGG 4 cut(s) 14, 651, 1837, 2362
HphI GGTGA 6 cut(s) 487, 634, 1510, 2119, 2333, 2428
Hpy166II GTNNAC 5 cut(s) 367, 448, 478, 1172, 1958
Hpy8I GTNNAC 5 cut(s) 367, 448, 478, 1172, 1958
HpyAV CCTTC 9 cut(s) 583, 853, 860, 888, 1464, 1487, 1676, 1801, 2116
HpyCH4III ACNGT 7 cut(s) 189, 568, 742, 885, 1047, 2072, 2086
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 65, 167, 358, 539, 1540, 1717
HpyF3I CTNAG 6 cut(s) 212, 760, 1202, 1535, 1871, 2207
Hsp92II CATG 9 cut(s) 160, 327, 490, 1416, 1529, 1720, 1772, 2018, 2514
Ksp22I TGATCA 2 cut(s) 1593, 1974
KspI CCGCGG 1 cut(s) 396
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 5 cut(s) 586, 652, 1540, 1759, 2401
Lsp1109I GCAGC 7 cut(s) 342, 345, 364, 831, 1281, 2041, 2364
LweI GCATC 9 cut(s) 1024, 1070, 1267, 1351, 1484, 1729, 1920, 2193, 2286
MaeI CTAG 7 cut(s) 41, 137, 459, 1181, 1565, 1814, 2030
MaeIII GTNAC 7 cut(s) 150, 562, 736, 967, 1505, 1516, 2125
MbiI CCGCTC 1 cut(s) 647
MfeI CAATTG 1 cut(s) 347
MflI RGATCY 5 cut(s) 79, 283, 334, 977, 1567
MhlI GDGCHC 6 cut(s) 266, 642, 1082, 1174, 1545, 1849
MlyI GAGTC 3 cut(s) 1456, 1700, 1963
MmeI TCCRAC 1 cut(s) 550
Mph1103I ATGCAT 2 cut(s) 1722, 2318
MroXI GAANNNNTTC 3 cut(s) 228, 921, 1047
MseI TTAA 7 cut(s) 9, 957, 1013, 1146, 1904, 2283, 2336
MslI CAYNNNNRTG 2 cut(s) 1148, 1946
MspA1I CMGCKG 2 cut(s) 355, 395
MspI CCGG 4 cut(s) 14, 651, 1837, 2362
MspR9I CCNGG 5 cut(s) 133, 651, 1838, 2142, 2382
MunI CAATTG 1 cut(s) 347
Mva1269I GAATGC 3 cut(s) 835, 944, 2318
MvaI CCWGG 3 cut(s) 133, 2142, 2382
MvnI CGCG 3 cut(s) 395, 992, 2094
MwoI GCNNNNNNNGC 7 cut(s) 56, 65, 167, 358, 539, 1540, 1717
NciI CCSGG 2 cut(s) 651, 1838
NdeI CATATG 1 cut(s) 1604
NlaIII CATG 9 cut(s) 160, 327, 490, 1416, 1529, 1720, 1772, 2018, 2514
NlaIV GGNNCC 2 cut(s) 1761, 2294
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 4 cut(s) 150, 967, 1516, 2125
NsiI ATGCAT 2 cut(s) 1722, 2318
NspI RCATGY 2 cut(s) 1720, 1772
NspV TTCGAA 3 cut(s) 256, 746, 1703
PaeI GCATGC 1 cut(s) 1720
PaeR7I CTCGAG 1 cut(s) 467
PagI TCATGA 1 cut(s) 486
PaqCI CACCTGC 2 cut(s) 352, 427
PciSI GCTCTTC 1 cut(s) 240
PctI GAATGC 3 cut(s) 835, 944, 2318
PdmI GAANNNNTTC 3 cut(s) 228, 921, 1047
PfeI GAWTC 6 cut(s) 372, 764, 917, 961, 1896, 2450
PflFI GACNNNGTC 1 cut(s) 1203
PflMI CCANNNNNTGG 1 cut(s) 1629
PfoI TCCNGGA 2 cut(s) 649, 2140
PkrI GCNGC 8 cut(s) 354, 357, 360, 846, 1271, 2056, 2379, 2508
PleI GAGTC 3 cut(s) 1455, 1699, 1962
PpsI GAGTC 3 cut(s) 1455, 1699, 1962
PpuMI RGGWCCY 1 cut(s) 2293
PshBI ATTAAT 1 cut(s) 9
Psp124BI GAGCTC 3 cut(s) 1082, 1545, 1849
Psp5II RGGWCCY 1 cut(s) 2293
Psp6I CCWGG 3 cut(s) 131, 2140, 2380
PspEI GGTNACC 2 cut(s) 1505, 2125
PspFI CCCAGC 2 cut(s) 1712, 2289
PspGI CCWGG 3 cut(s) 131, 2140, 2380
PspN4I GGNNCC 2 cut(s) 1761, 2294
PspPI GGNCC 7 cut(s) 390, 1321, 1380, 1710, 2138, 2293, 2504
PspPPI RGGWCCY 1 cut(s) 2293
PstI CTGCAG 1 cut(s) 363
PstNI CAGNNNCTG 4 cut(s) 187, 358, 548, 844
PsuI RGATCY 5 cut(s) 79, 283, 334, 977, 1567
PsyI GACNNNGTC 1 cut(s) 1203
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 7 cut(s) 299, 368, 463, 1740, 1812, 1869, 2088
RsaNI GTAC 7 cut(s) 298, 367, 462, 1739, 1811, 1868, 2087
RseI CAYNNNNRTG 2 cut(s) 1148, 1946
SacI GAGCTC 3 cut(s) 1082, 1545, 1849
SacII CCGCGG 1 cut(s) 396
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 7 cut(s) 9, 957, 1013, 1146, 1904, 2283, 2336
SatI GCNGC 8 cut(s) 353, 356, 359, 845, 1270, 2055, 2378, 2507
Sau96I GGNCC 7 cut(s) 390, 1321, 1380, 1710, 2138, 2293, 2504
ScaI AGTACT 3 cut(s) 463, 1869, 2088
SchI GAGTC 3 cut(s) 1456, 1700, 1963
ScrFI CCNGG 5 cut(s) 133, 651, 1838, 2142, 2382
SduI GDGCHC 6 cut(s) 266, 642, 1082, 1174, 1545, 1849
SfaNI GCATC 9 cut(s) 1024, 1070, 1267, 1351, 1484, 1729, 1920, 2193, 2286
SfcI CTRYAG 3 cut(s) 289, 359, 423
Sfr274I CTCGAG 1 cut(s) 467
Sfr303I CCGCGG 1 cut(s) 396
SfuI TTCGAA 3 cut(s) 256, 746, 1703
SgrBI CCGCGG 1 cut(s) 396
SinI GGWCC 5 cut(s) 390, 1321, 1380, 2138, 2293
SlaI CTCGAG 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 1148, 1946
SmlI CTYRAG 4 cut(s) 467, 801, 2255, 2350
SmoI CTYRAG 4 cut(s) 467, 801, 2255, 2350
SpeI ACTAGT 1 cut(s) 1813
SphI GCATGC 1 cut(s) 1720
SsiI CCGC 7 cut(s) 277, 393, 395, 645, 990, 2094, 2507
SspI AATATT 1 cut(s) 955
SspMI CTAG 7 cut(s) 41, 137, 459, 1181, 1565, 1814, 2030
SstI GAGCTC 3 cut(s) 1082, 1545, 1849
StyD4I CCNGG 5 cut(s) 131, 649, 1836, 2140, 2380
StyI CCWWGG 2 cut(s) 672, 1324
TaaI ACNGT 7 cut(s) 189, 568, 742, 885, 1047, 2072, 2086
TaqI TCGA 9 cut(s) 256, 468, 746, 787, 1478, 1559, 1703, 2243, 2346
TaqII GACCGA 1 cut(s) 162
TatI WGTACW 4 cut(s) 297, 461, 1867, 2086
TauI GCSGC 1 cut(s) 2509
TfiI GAWTC 6 cut(s) 372, 764, 917, 961, 1896, 2450
Tru1I TTAA 7 cut(s) 9, 957, 1013, 1146, 1904, 2283, 2336
Tru9I TTAA 7 cut(s) 9, 957, 1013, 1146, 1904, 2283, 2336
TscAI CASTG 4 cut(s) 61, 845, 888, 2077
TseFI GTSAC 4 cut(s) 150, 967, 1516, 2125
TseI GCWGC 7 cut(s) 352, 355, 358, 844, 1269, 2054, 2377
Tsp45I GTSAC 4 cut(s) 150, 967, 1516, 2125
TspDTI ATGAA 9 cut(s) 503, 837, 1212, 1320, 1401, 1416, 1446, 1670, 1911
TspGWI ACGGA 2 cut(s) 384, 1975
TspRI CASTG 4 cut(s) 61, 845, 888, 2077
Tth111I GACNNNGTC 1 cut(s) 1203
Van91I CCANNNNNTGG 1 cut(s) 1629
VneI GTGCAC 1 cut(s) 1170
VpaK11BI GGWCC 5 cut(s) 390, 1321, 1380, 2138, 2293
VspI ATTAAT 1 cut(s) 9
XagI CCTNNNNNAGG 1 cut(s) 2120
XbaI TCTAGA 2 cut(s) 1180, 2029
XceI RCATGY 2 cut(s) 1720, 1772
XcmI CCANNNNNNNNNTGG 1 cut(s) 1150
XhoI CTCGAG 1 cut(s) 467
XmiI GTMKAC 1 cut(s) 1957
XmnI GAANNNNTTC 3 cut(s) 228, 921, 1047
XspI CTAG 7 cut(s) 41, 137, 459, 1181, 1565, 1814, 2030
ZrmI AGTACT 3 cut(s) 463, 1869, 2088
Zsp2I ATGCAT 2 cut(s) 1722, 2318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.