FvH4_4g31860

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000039
Physical Location & Seq
Forward (+)
30794267 .. 30797641
3375 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31860.t3

Sequence Viewer

Length: 2424 bp
ATGAGTATTGATCCGGTGGAGCTTGCTTTAGGTGCTGTTCTAGGAACAGCATTCAGTGTACTCTATGCTGGCGTTGAAGATCTGATTGCCAAGAACCACTTGTTCAGAGGTCTCTTCAAAGACATCAAATCCAAGCTAGACACCCTGAAGCCACTCCTCGCCGATATGACACTGTCTAACAAGCAATTGTTGTACGCAAACAACGATGAGCTTAGAGGTTTAGAACAAGTACTCAAGGCCGGTGAAGAGCTCGTTCTGAAGTGCTCAAAGGTGCACGGAATCAGGGATGGGAAGAGGACTGCGGTGGGGGTTAGCCAAATATCTAGTACTCTGGAGGAAGTGAACCAACGCATGAAAGAGAATTTTATGATACAAGACCAATCTGCTTGGTGTGGAGTACCTGCACTTCCGGAGATTATAGTTGGATTGGAAGCTCCATTGGAGAAATTGAAGACAGAGCTTCTTGGGAATGAAGGATTCTCAATGCTTGTGCTCACTGCTCCGGGTGGATGTGGGAAAACTACCTTGGCAACAAAGTTTTGCACAGATCAGGAAGTCAAAGATAAATTCGGTGAAAATATCTACTTTGTCACTGTTTCCAAGAAGTATAGCTTAAATTCAATTGTAGAACAACTATATCGACGCAAGGGTTGTCAAGTTCCTACTTTCCAGAACGAAGTGAATGCAGTTCAAGGGCTGCAACAGTTTTTAAAGGATGAAGGAGGGAAACCCTTACTGTTGGTCTTGGATGATGTTTGGTCCGGATCAGATTCTCTTCTTCACAAATTTGATTGCAAAATGGCAAAAATGAAGATTCTGGTGACATCAAGATCTGAATTTCGAGCATTCTGTCCTCCATATCGTCTAGAAATGTTGGGTTGTGACGATGCAATGAAACTTTTTCATCATTCAGCATCCTTGGGAGATAAGAGCTCTCATATTCCACTAAAGCTTGCCAGAAAGATAGTAGAGCGTTGCAAGGGATTTCCACTTGCCATTACAGTGGTGGGCAGATCACTTTGTGCACAACCTATAGAGATGTGGCAAGACAGACTAAATCAATGGTCTAAAGGTTCATCTATTTTTGATTATGAGACTGATTTACTTCTTTCCCTCCAAAGCAGCTTGGATGCTTTGGACAAGGAAAGGGCTATCATCAAACAATGCTTCTTGGACCTTGGTTCATTTCCTGAAGACAAAAGAATCCCTGTTAATGCCCTTATTGATATATGGTCTGAGTTATATGATGATATACATGAAGACACTTCGTGTATTGCAGCCCTCTACGAACTCAACAACCGGAGCCTGGCTAATCTTACAGACAATAGGGCGGGGAAATTGGATGGGGAAGGATATTATGATGACCACTATGTCACCCAACATGATTTGCTTAGGGAGCTGGCTATTTTTCACTCGAAGCTAGAATCGGCAGAGCTTAGAGAAAGATTGTTCATAGACATATGTGGAAGCAATCTTCCCAAATGGTGGAGGGAACAAAAGTATCAAGCTTTGAAAGCTCGCCTTCTATCTATATCAACTGATGGAGAGTCTTCAACTTTGAAATGGCCAGACATGCATCTGCCAGAAACAGAGGTACTAGTGTTGAATTTGCGGGAGATAGAGAAGTATGCTATACCCGAATTTGTGGAGAAAATGGAGAATTTGAAGGTACTAGTAATCACAAATGAGGATTTGCCGAGATGTGAGTTGACCAATCCTCAACTATTAAGTTGGTTCTCCAATCTAAAGAGAATGAGATTAGAGGGCCTCTCATTATCATCCATATTAAAGAACTCCATACAAGTGAAGAGTCTTCGGAAGTTGTCTTTGATCAAATGCGAAACTGAAGATATCAGTTCAGAAAAGTTTTCCCATGCTTTTCCAAATCTTGAAGAGATGTACATGGACCAAACTGGTATTTTGAAGCCATTATTTCGCGGGTTATGTGATCTGATTTCCCTAAAGAAGCTCACCATCTGCCTGGCACATGGAATAGAAGTTCCTGACGAGATTGGAAAGTTGGTCAATTTAGAAGAATTGAGGTTGATGTCATCGATTGGGCCTTCAAAGTTTCCAGACTCGATCAGGAACCTCAAAAAGTTGAACACTCTTTGCGTGTTAGATTGCATGTCCTTGAATGAGTTACCTGAATGTATTGGTGAAATGAGGAGTCTAAGAAAGATGGACACGAGGGGTTGCGACGAGCTTAAGAAGCTGCCGCTATCAATACTGAATTTGAAGCAGTTAGAGGAGGTCATATTTGATGAATATACCGAAAGCGCATGGCAACCCCTGTTGCCCAGTCTCAGTGACAAAAACAGAGACATACACATTGTTGTTGCGGCCATAGATCCCAGAAGACTGGAAAAAAGAAGTGCTGAGATGTGGTTCTTCGCCGTAGAGAGATTATACATGCGAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

808

Amino Acids

91.61

Weight (kDa)

5.74

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 96 3.1e-11 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 144 - 304 8.2e-19 NB-ARC domain
LRR_14 PF23598 674 - 799 1.2e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1370
Acc36I ACCTGC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 1485
AccII CGCG 1 cut(s) 1938
AccIII TCCGGA 2 cut(s) 409, 761
AciI CCGC 6 cut(s) 302, 1331, 1612, 1938, 2219, 2342
AclWI GGATC 3 cut(s) 5, 772, 2345
AcoI YGGCCR 2 cut(s) 1565, 2343
AcuI CTGAAG 4 cut(s) 167, 278, 1212, 1866
AdeI CACNNNGTG 2 cut(s) 1022, 1269
AfaI GTAC 8 cut(s) 60, 194, 231, 328, 399, 1596, 1671, 1901
AfiI CCNNNNNNNGG 2 cut(s) 1306, 1485
AflII CTTAAG 1 cut(s) 2207
AhlI ACTAGT 2 cut(s) 1597, 1672
AjnI CCWGG 2 cut(s) 1305, 1980
AjuI GAANNNNNNNTTGG 2 cut(s) 509, 541
AloI GAACNNNNNNTCC 2 cut(s) 1983, 2015
Alw21I GWGCWC 6 cut(s) 252, 266, 276, 495, 935, 1027
Alw26I GTCTC 4 cut(s) 116, 1088, 2309, 2316
Alw44I GTGCAC 2 cut(s) 272, 1023
AlwI GGATC 3 cut(s) 5, 772, 2345
Aor13HI TCCGGA 2 cut(s) 409, 761
AoxI GGCC 5 cut(s) 237, 1565, 1765, 2060, 2343
ApaLI GTGCAC 2 cut(s) 272, 1023
ApeKI GCWGC 4 cut(s) 697, 1122, 1277, 2215
AspLEI GCGC 1 cut(s) 2282
AspS9I GGNCC 5 cut(s) 759, 1174, 1765, 1906, 2060
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 6 cut(s) 254, 584, 832, 1366, 1963, 2171
AvaII GGWCC 3 cut(s) 759, 1174, 1906
BaeGI GKGCMC 2 cut(s) 276, 1027
BaeI ACNNNNGTAYC 4 cut(s) 1484, 1517, 1586, 1619
BalI TGGCCA 1 cut(s) 1567
BanII GRGCYC 2 cut(s) 252, 935
BarI GAAGNNNNNNTAC 2 cut(s) 390, 422
BauI CACGAG 1 cut(s) 2188
BbsI GAAGAC 6 cut(s) 458, 1200, 1266, 1542, 1805, 2365
Bbv12I GWGCWC 6 cut(s) 252, 266, 276, 495, 935, 1027
BbvI GCAGC 4 cut(s) 684, 1134, 1289, 2202
BccI CCATC 5 cut(s) 281, 1337, 1535, 1982, 2176
BceAI ACGGC 1 cut(s) 2381
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BciT130I CCWGG 2 cut(s) 1307, 1982
BclI TGATCA 1 cut(s) 1830
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 4 cut(s) 116, 1088, 2309, 2316
BcuI ACTAGT 2 cut(s) 1597, 1672
BfaI CTAG 7 cut(s) 41, 137, 324, 866, 1421, 1598, 1673
BfmI CTRYAG 1 cut(s) 1032
BfrI CTTAAG 1 cut(s) 2207
BfuAI ACCTGC 1 cut(s) 409
BglII AGATCT 2 cut(s) 79, 830
BisI GCNGC 6 cut(s) 698, 1123, 1278, 2216, 2219, 2343
BlsI GCNGC 6 cut(s) 699, 1124, 1279, 2217, 2220, 2344
BmcAI AGTACT 2 cut(s) 231, 328
Bme1390I CCNGG 3 cut(s) 504, 1307, 1982
Bme18I GGWCC 3 cut(s) 759, 1174, 1906
BmgT120I GGNCC 5 cut(s) 759, 1174, 1765, 1906, 2060
BmiI GGNNCC 2 cut(s) 1304, 2090
BmrFI CCNGG 3 cut(s) 504, 1307, 1982
BmrI ACTGGG 1 cut(s) 2295
BmsI GCATC 4 cut(s) 877, 923, 1120, 1585
BmuI ACTGGG 1 cut(s) 2295
BpiI GAAGAC 6 cut(s) 458, 1200, 1266, 1542, 1805, 2365
BplI GAGNNNNNCTC 2 cut(s) 1754, 1786
BpmI CTGGAG 1 cut(s) 353
Bpu10I CCTNAGC 1 cut(s) 1391
BpuEI CTTGAG 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 504
Bsa29I ATCGAT 1 cut(s) 2054
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 3 cut(s) 525, 918, 1177
BsaWI WCCGGW 4 cut(s) 13, 409, 761, 1299
Bsc4I CCNNNNNNNGG 2 cut(s) 1306, 1485
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 3 cut(s) 1918, 2301, 2367
Bse3DI GCAATG 1 cut(s) 897
BseAI TCCGGA 2 cut(s) 409, 761
BseBI CCWGG 2 cut(s) 1307, 1982
BseCI ATCGAT 1 cut(s) 2054
BseDI CCNNGG 3 cut(s) 525, 918, 1177
BseGI GGATG 8 cut(s) 292, 515, 721, 754, 914, 1135, 1348, 1778
BseLI CCNNNNNNNGG 2 cut(s) 1306, 1485
BseMI GCAATG 1 cut(s) 897
BseMII CTCAG 3 cut(s) 1227, 2320, 2370
BseNI ACTGG 3 cut(s) 1918, 2301, 2367
BseRI GAGGAG 3 cut(s) 146, 2182, 2264
BseSI GKGCMC 2 cut(s) 276, 1027
BseXI GCAGC 4 cut(s) 684, 1134, 1289, 2202
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 1938
BshFI GGCC 5 cut(s) 239, 1567, 1767, 2062, 2345
BshVI ATCGAT 1 cut(s) 2054
BsiHKAI GWGCWC 6 cut(s) 252, 266, 276, 495, 935, 1027
BsiSI CCGG 6 cut(s) 14, 240, 410, 503, 762, 1300
BslI CCNNNNNNNGG 2 cut(s) 1306, 1485
BsmAI GTCTC 4 cut(s) 116, 1088, 2309, 2316
BsmI GAATGC 3 cut(s) 50, 688, 845
BsnI GGCC 5 cut(s) 239, 1567, 1767, 2062, 2345
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 6 cut(s) 252, 266, 276, 495, 935, 1027
Bsp13I TCCGGA 2 cut(s) 409, 761
Bsp1407I TGTACA 1 cut(s) 1899
BspACI CCGC 6 cut(s) 302, 1331, 1612, 1938, 2219, 2342
BspANI GGCC 5 cut(s) 239, 1567, 1767, 2062, 2345
BspCNI CTCAG 3 cut(s) 1228, 2319, 2371
BspDI ATCGAT 1 cut(s) 2054
BspEI TCCGGA 2 cut(s) 409, 761
BspFNI CGCG 1 cut(s) 1938
BspLI GGNNCC 2 cut(s) 1304, 2090
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 3 cut(s) 5, 772, 2345
BspQI GCTCTTC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 2207
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 897
BsrFI RCCGGY 1 cut(s) 239
BsrGI TGTACA 1 cut(s) 1899
BsrI ACTGG 3 cut(s) 1918, 2301, 2367
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 3 cut(s) 525, 918, 1177
BssSI CACGAG 1 cut(s) 2188
BssT1I CCWWGG 3 cut(s) 525, 918, 1177
Bst2BI CACGAG 1 cut(s) 2188
Bst2UI CCWGG 2 cut(s) 1307, 1982
Bst4CI ACNGT 5 cut(s) 174, 595, 705, 738, 1003
Bst6I CTCTTC 6 cut(s) 119, 240, 287, 780, 1802, 1887
BstAFI CTTAAG 1 cut(s) 2207
BstAUI TGTACA 1 cut(s) 1899
BstC8I GCNNGC 5 cut(s) 24, 70, 954, 1401, 1519
BstDEI CTNAG 7 cut(s) 212, 1236, 1391, 1436, 2174, 2306, 2379
BstF5I GGATG 8 cut(s) 292, 515, 721, 754, 914, 1135, 1348, 1778
BstFNI CGCG 1 cut(s) 1938
BstHHI GCGC 1 cut(s) 2282
BstMAI GTCTC 4 cut(s) 116, 1088, 2309, 2316
BstMWI GCNNNNNNNGC 5 cut(s) 32, 1396, 1514, 1573, 2212
BstNI CCWGG 2 cut(s) 1307, 1982
BstNSI RCATGY 3 cut(s) 1576, 2131, 2416
BstSCI CCNGG 3 cut(s) 502, 1305, 1980
BstSFI CTRYAG 1 cut(s) 1032
BstSLI GKGCMC 2 cut(s) 276, 1027
BstUI CGCG 1 cut(s) 1938
BstV1I GCAGC 4 cut(s) 684, 1134, 1289, 2202
BstV2I GAAGAC 6 cut(s) 458, 1200, 1266, 1542, 1805, 2365
BstX2I RGATCY 3 cut(s) 79, 830, 2350
BstXI CCANNNNNNTGG 3 cut(s) 1003, 1981, 2362
BstYI RGATCY 3 cut(s) 79, 830, 2350
Bsu15I ATCGAT 1 cut(s) 2054
BsuRI GGCC 5 cut(s) 239, 1567, 1767, 2062, 2345
BsuTUI ATCGAT 1 cut(s) 2054
BtsCI GGATG 8 cut(s) 292, 515, 721, 754, 914, 1135, 1348, 1778
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 6 cut(s) 61, 170, 495, 591, 1008, 2314
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 5 cut(s) 24, 70, 954, 1401, 1519
CfoI GCGC 1 cut(s) 2282
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 5 cut(s) 759, 1174, 1765, 1906, 2060
ClaI ATCGAT 1 cut(s) 2054
CseI GACGC 1 cut(s) 651
Csp6I GTAC 8 cut(s) 59, 193, 230, 327, 398, 1595, 1670, 1900
CviQI GTAC 8 cut(s) 59, 193, 230, 327, 398, 1595, 1670, 1900
DdeI CTNAG 7 cut(s) 212, 1236, 1391, 1436, 2174, 2306, 2379
DraI TTTAAA 1 cut(s) 711
DraIII CACNNNGTG 2 cut(s) 1022, 1269
DrdI GACNNNNNNGTC 1 cut(s) 1370
DseDI GACNNNNNNGTC 1 cut(s) 1370
EaeI YGGCCR 2 cut(s) 1565, 2343
Eam1104I CTCTTC 6 cut(s) 119, 240, 287, 780, 1802, 1887
EarI CTCTTC 6 cut(s) 119, 240, 287, 780, 1802, 1887
Ecl136II GAGCTC 2 cut(s) 250, 933
Eco130I CCWWGG 3 cut(s) 525, 918, 1177
Eco24I GRGCYC 2 cut(s) 252, 935
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 1 cut(s) 1852
Eco47I GGWCC 3 cut(s) 759, 1174, 1906
Eco53kI GAGCTC 2 cut(s) 250, 933
Eco57I CTGAAG 4 cut(s) 167, 278, 1212, 1866
EcoICRI GAGCTC 2 cut(s) 250, 933
EcoO109I RGGNCCY 1 cut(s) 1765
EcoRII CCWGG 2 cut(s) 1305, 1980
EcoRV GATATC 1 cut(s) 1852
EcoT14I CCWWGG 3 cut(s) 525, 918, 1177
EcoT22I ATGCAT 1 cut(s) 1578
EcoT38I GRGCYC 2 cut(s) 252, 935
ErhI CCWWGG 3 cut(s) 525, 918, 1177
FalI AAGNNNNNCTT 6 cut(s) 83, 115, 596, 628, 1506, 1538
FauI CCCGC 3 cut(s) 1324, 1605, 1931
FauNDI CATATG 1 cut(s) 1460
FbaI TGATCA 1 cut(s) 1830
Fnu4HI GCNGC 6 cut(s) 698, 1123, 1278, 2216, 2219, 2343
FokI GGATG 8 cut(s) 299, 522, 728, 761, 901, 1142, 1355, 1765
FriOI GRGCYC 2 cut(s) 252, 935
Fsp4HI GCNGC 6 cut(s) 698, 1123, 1278, 2216, 2219, 2343
FspBI CTAG 7 cut(s) 41, 137, 324, 866, 1421, 1598, 1673
GlaI GCGC 1 cut(s) 2281
GluI GCNGC 6 cut(s) 698, 1123, 1278, 2216, 2219, 2343
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 5 cut(s) 239, 1567, 1767, 2062, 2345
HapII CCGG 6 cut(s) 14, 240, 410, 503, 762, 1300
HgaI GACGC 1 cut(s) 651
HhaI GCGC 1 cut(s) 2282
Hin6I GCGC 1 cut(s) 2280
HinP1I GCGC 1 cut(s) 2280
HincII GTYRAC 1 cut(s) 1710
HindII GTYRAC 1 cut(s) 1710
HindIII AAGCTT 2 cut(s) 950, 1506
HpaII CCGG 6 cut(s) 14, 240, 410, 503, 762, 1300
HphI GGTGA 6 cut(s) 254, 584, 832, 1366, 1963, 2171
Hpy166II GTNNAC 5 cut(s) 59, 274, 343, 1025, 1710
Hpy188I TCNGA 9 cut(s) 84, 107, 258, 769, 835, 1237, 1818, 1861, 1953
Hpy8I GTNNAC 5 cut(s) 59, 274, 343, 1025, 1710
Hpy99I CGWCG 2 cut(s) 645, 2204
HpyAV CCTTC 6 cut(s) 467, 713, 1343, 1532, 1660, 2073
HpyCH4III ACNGT 5 cut(s) 174, 595, 705, 738, 1003
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 1396, 1514, 1573, 2212
HpyF3I CTNAG 7 cut(s) 212, 1236, 1391, 1436, 2174, 2306, 2379
HspAI GCGC 1 cut(s) 2280
Kpn2I TCCGGA 2 cut(s) 409, 761
Ksp22I TGATCA 1 cut(s) 1830
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 5 cut(s) 19, 439, 505, 1302, 1396
Lsp1109I GCAGC 4 cut(s) 684, 1134, 1289, 2202
LweI GCATC 4 cut(s) 877, 923, 1120, 1585
MaeI CTAG 7 cut(s) 41, 137, 324, 866, 1421, 1598, 1673
MaeIII GTNAC 6 cut(s) 589, 820, 881, 1372, 2142, 2309
MfeI CAATTG 2 cut(s) 185, 621
MflI RGATCY 3 cut(s) 79, 830, 2350
MhlI GDGCHC 6 cut(s) 252, 266, 276, 495, 935, 1027
MlsI TGGCCA 1 cut(s) 1567
MluNI TGGCCA 1 cut(s) 1567
MlyI GAGTC 4 cut(s) 1556, 1819, 2072, 2179
MmeI TCCRAC 1 cut(s) 403
Mox20I TGGCCA 1 cut(s) 1567
Mph1103I ATGCAT 1 cut(s) 1578
MroI TCCGGA 2 cut(s) 409, 761
MscI TGGCCA 1 cut(s) 1567
MseI TTAA 7 cut(s) 614, 710, 1212, 1727, 1787, 2208, 2422
MslI CAYNNNNRTG 2 cut(s) 1001, 1802
Msp20I TGGCCA 1 cut(s) 1567
MspCI CTTAAG 1 cut(s) 2207
MspI CCGG 6 cut(s) 14, 240, 410, 503, 762, 1300
MspR9I CCNGG 3 cut(s) 504, 1307, 1982
MunI CAATTG 2 cut(s) 185, 621
Mva1269I GAATGC 3 cut(s) 50, 688, 845
MvaI CCWGG 2 cut(s) 1307, 1982
MvnI CGCG 1 cut(s) 1938
MwoI GCNNNNNNNGC 5 cut(s) 32, 1396, 1514, 1573, 2212
NciI CCSGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 1460
NlaIV GGNNCC 2 cut(s) 1304, 2090
NmeAIII GCCGAG 1 cut(s) 1722
NmuCI GTSAC 5 cut(s) 589, 820, 881, 1372, 2309
NsiI ATGCAT 1 cut(s) 1578
NspI RCATGY 3 cut(s) 1576, 2131, 2416
PciSI GCTCTTC 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 3 cut(s) 50, 688, 845
PfeI GAWTC 6 cut(s) 279, 477, 770, 814, 1203, 1424
PflFI GACNNNGTC 1 cut(s) 172
PflMI CCANNNNNTGG 1 cut(s) 1485
PkrI GCNGC 6 cut(s) 699, 1124, 1279, 2217, 2220, 2344
PleI GAGTC 4 cut(s) 1555, 1818, 2072, 2178
PpsI GAGTC 4 cut(s) 1555, 1818, 2072, 2178
Psp124BI GAGCTC 2 cut(s) 252, 935
Psp6I CCWGG 2 cut(s) 1305, 1980
PspGI CCWGG 2 cut(s) 1305, 1980
PspN4I GGNNCC 2 cut(s) 1304, 2090
PspPI GGNCC 5 cut(s) 759, 1174, 1765, 1906, 2060
PsuI RGATCY 3 cut(s) 79, 830, 2350
PsyI GACNNNGTC 1 cut(s) 172
RsaI GTAC 8 cut(s) 60, 194, 231, 328, 399, 1596, 1671, 1901
RsaNI GTAC 8 cut(s) 59, 193, 230, 327, 398, 1595, 1670, 1900
RseI CAYNNNNRTG 2 cut(s) 1001, 1802
SacI GAGCTC 2 cut(s) 252, 935
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 7 cut(s) 614, 710, 1212, 1727, 1787, 2208, 2422
SatI GCNGC 6 cut(s) 698, 1123, 1278, 2216, 2219, 2343
Sau96I GGNCC 5 cut(s) 759, 1174, 1765, 1906, 2060
ScaI AGTACT 2 cut(s) 231, 328
SchI GAGTC 4 cut(s) 1556, 1819, 2072, 2179
ScrFI CCNGG 3 cut(s) 504, 1307, 1982
SduI GDGCHC 6 cut(s) 252, 266, 276, 495, 935, 1027
SfaNI GCATC 4 cut(s) 877, 923, 1120, 1585
SfcI CTRYAG 1 cut(s) 1032
SinI GGWCC 3 cut(s) 759, 1174, 1906
SmiMI CAYNNNNRTG 2 cut(s) 1001, 1802
SmlI CTYRAG 2 cut(s) 233, 2207
SmoI CTYRAG 2 cut(s) 233, 2207
SpeI ACTAGT 2 cut(s) 1597, 1672
SsiI CCGC 6 cut(s) 302, 1331, 1612, 1938, 2219, 2342
SspMI CTAG 7 cut(s) 41, 137, 324, 866, 1421, 1598, 1673
SstI GAGCTC 2 cut(s) 252, 935
StyD4I CCNGG 3 cut(s) 502, 1305, 1980
StyI CCWWGG 3 cut(s) 525, 918, 1177
TaaI ACNGT 5 cut(s) 174, 595, 705, 738, 1003
TaqI TCGA 5 cut(s) 640, 841, 1415, 2054, 2081
TatI WGTACW 4 cut(s) 58, 229, 326, 1899
TauI GCSGC 2 cut(s) 2221, 2345
TfiI GAWTC 6 cut(s) 279, 477, 770, 814, 1203, 1424
Tru1I TTAA 7 cut(s) 614, 710, 1212, 1727, 1787, 2208, 2422
Tru9I TTAA 7 cut(s) 614, 710, 1212, 1727, 1787, 2208, 2422
TscAI CASTG 6 cut(s) 61, 177, 502, 598, 1008, 2314
TseFI GTSAC 5 cut(s) 589, 820, 881, 1372, 2309
TseI GCWGC 4 cut(s) 697, 1122, 1277, 2215
Tsp45I GTSAC 5 cut(s) 589, 820, 881, 1372, 2309
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 6 cut(s) 61, 177, 502, 598, 1008, 2314
Tth111I GACNNNGTC 1 cut(s) 172
Van91I CCANNNNNTGG 1 cut(s) 1485
Vha464I CTTAAG 1 cut(s) 2207
VneI GTGCAC 2 cut(s) 272, 1023
VpaK11BI GGWCC 3 cut(s) 759, 1174, 1906
XbaI TCTAGA 1 cut(s) 865
XceI RCATGY 3 cut(s) 1576, 2131, 2416
XcmI CCANNNNNNNNNTGG 1 cut(s) 1003
XspI CTAG 7 cut(s) 41, 137, 324, 866, 1421, 1598, 1673
ZrmI AGTACT 2 cut(s) 231, 328
Zsp2I ATGCAT 1 cut(s) 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.