FvH4_6g50110

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000039
Physical Location & Seq
Reverse (-)
37350460 .. 37354090
3631 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50110.t1

Sequence Viewer

Length: 2787 bp
ATGGCGGTTGTGGGGGATTTTCTTGTAGGGAAAGCACTCGAGTTACTGTATGGTGGTGTCGAAACGTTGATTGCCAATAATCTACTCTTTGAAGCTCTCTCCACCGACATCAAGGCCAAGCTAGATGCTTTGAGACCGATGATCCAAAAGATGGTACAACGTAATCATCAGCGACCGGATGGTCCAGAGGATCCAGTCCTAAGAGATTTACGAGTATTAATGGAGGAAGGTGCAGTTGTCGTCTCCAACTGCTGCTCTAAGATTCGTATTTGGAACTTCTATAAGAAGTACAAGTACACCAACAAGCTTCTCAAGCTGAATGAAGCTCTTGAGAAGCAGCTGAGTTTACTGAATGTGCGTGTAGCAGTTGTTACAGACGAGAGCAATGGCATGCTGCATGAGCTCTTAGATATTGCGAATCAAAACAAATCTGCAGGAATTGAAGCAGGTTGTGCACTACCTGAACTACCGTCACTTATAGTTGGATTGGATATTCCACTGAAGGAATTGAAGACGGAGTTTTTTAAGGATGAAGTATCACTGCTTGTATTGACTGCTCCAGGAGGATGTGGGAAAACTACTCTGGCAACAAAGTTCTGTCAAGATCAAGACGTCAAAGATAAGTTCAAGGACAACATCTTCTTCGCCACTGTTTCTAAAAAGCCTAAGCTGGAGCTTATTATGACAGAGCTATACAAACAAAAGGATTATAAGGTACCTACTTTCCAGAATGGAGTATTTCCAGTGAAATGGCTACAACAAATTGTGGAGAAAGAAGGTGATATTCCTTTATTGTTGGTGTTGGATGACGTTTGGTCCGGATCAGAATCTCTTATTCAGAAATTTGAATTTACGATGTCAAATTACAAGATTTTAGTGACATCAAGGTCTGAGTTTCCGAGATTTGGCCCTTCATATCATCTCCAACTATTAGATGATGATAATGCAATGCAACTTTTTCATCACACAGCATTCTTAGGAGACAAGAGCTCTCATATCCCAAACGAACTTGCAAAAAAGATAGTAAAACACTGTAATGGATTTCCACTGGCTATTACAGTGGTAGGAAGATCACTTTGCAGTCAACCTATACAAACGTGGCGAAGTAGGGTAAATGAATGGTCAAAAGGTGCTTCAGTACTCGACTGTGAGATGGAATTGTTTCAATGCCTCCAAAGCAGCTTTGATGCCTTGGATAATGCAACGCCTAAGGTCAAGGATTGTTTGTTAGACCTAGGTGCGTTTCCAGAAGATTCTGAAATCCAAGTTACAGCTCTCATTGATATGTGGGCGGAGTTGTATGATCTAGATGAAGAGATGTCATGCATTGCCGAACTCTATGAACTCAACAATCGCAGTCTAGCTCTTCTACAAGGGGATATGCGGTTGGAAGTTGTACAGGAGGCAATAGAATATAATAGATACGTTGTTAGGCAGCATGATTTGCTCAGAGAACTGGCTATCTACAACTCAAAACGTGATCCGATGGAGCATAGAGAAAGACTGATCATAGAGATATGTGGAAACAATGTTCCCAAATGGTGGAGAGAACAAAAATATCCATACATAAATGCTCGCCTTGTATCTATCTCAACTGATGGAGAGTTCTCAGTGAAACTGCCCGACATGGAATTACCAAAGGCAGAGGTTCTAGTCTTGAATTCTCAATCAGAAACGTCCTATGCCATACCCGAATTTGTGGAGAGAATGCATAAACTAAAGATGCTGCTTGTCATAAATTATACCAGCTGGGCAAAATTGGAGAATTTTCAACTACTGGGCTCCTTATCAAATCTCAGGAGTCTCAGATTAGACGGCATCAATCTTTCTAGCATAACCAAGAGCCCAGTACAGTTGAAGAATCTACACAAGTTATATTTGAGAGCATGTCGAGATGAGAGCAGAGATCTGAAACTATCACATATTATATTTCCAAATCTGAAGGAAATTGTTTTAGAAAGCTGCTCTTTCTGGAAATTGACAGGAGTTTTCTGCCAGCTGATTCATTTAACAAAGCTCAAAATCACTAGAAGACGCCGGTTATACTGCGAGTTGTCTGAAGCCATTGCAAATCTGGTCAGTTTAGAAGCGTTGACGCTAGACGACGTCGGCTTAGTTGCAGGGATTCTTGAATCGATTGGGAATCTCAGGAAATTAAAACTTCTTAAGATTTATAACGATCGGGATTTAGAGAAGTTGCCGGAACAGATGGGTATGCTTAAGTGTCTGAGACATCTCATCATCGAGGGTTGTAGTAGGCTATCTGCGTTGCCTGAATCAATTGGAAATTTGGTGAATCTAGAAGTATTGAGTCTAAGGTATTGTACCCTCTTGTCAGAGCTGCCGGAGTCGATCAGAAATCTCCAAAAGTTAAACTTTCTTGACATGTATAGATGCATAGAGATCGAGGAGTTGCCTGAACATTTTGGTGAATTGAAGAGTTTAATGAAGCTGCAAATGGTTGGTTGTTATGCATTGAGAAAACTGCCTGAATCAGTTTCAGATCTTGAGCAGTTAGAGCAAGTGATATGTGTAGATTGGGATGCAACACATCTATGGACACCCTTGTTGCCCACTCTCAAAAACCTTCGCATCGTCGTGCGGGAAGGCAGCAAATCCTTTGTCCCTTTTTGCCCAACTTCCCCTCGAAACTTCATTGGAACAGACCACAAAGGTCGAGCAAAACACAACAAAAAGAAAGCTAACAAAGCTGAAAGAGCAAGAGGGAAACTAAACACTGCTACAAACCACTACAGACAAAAAGCAAAGAAAGACAAAAGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

929

Amino Acids

106.05

Weight (kDa)

7.7

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 122 2.8e-11 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 168 - 325 3.1e-13 NB-ARC domain
LRR_14 PF23598 687 - 773 2e-09 Leucine-rich repeat region
LRR_14 PF23598 758 - 865 9.8e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 711, 2175
AasI GACNNNNNNGTC 2 cut(s) 180, 886
AatII GACGTC 2 cut(s) 615, 2109
Acc36I ACCTGC 1 cut(s) 437
Acc65I GGTACC 1 cut(s) 715
AccB1I GGYRCC 1 cut(s) 715
AccB7I CCANNNNNTGG 2 cut(s) 151, 1542
AccIII TCCGGA 1 cut(s) 818
AciI CCGC 4 cut(s) 5, 1292, 1384, 2602
AclI AACGTT 1 cut(s) 65
AclWI GGATC 5 cut(s) 136, 185, 198, 829, 1475
AcsI RAATTY 6 cut(s) 842, 848, 1660, 1694, 1765, 2287
AcuI CTGAAG 4 cut(s) 521, 1119, 1961, 2079
AcyI GRCGYC 3 cut(s) 612, 2035, 2106
AfaI GTAC 8 cut(s) 156, 290, 296, 717, 1140, 1398, 1851, 2326
AfiI CCNNNNNNNGG 3 cut(s) 151, 905, 1542
AflII CTTAAG 2 cut(s) 2165, 2219
AflIII ACRYGT 1 cut(s) 2385
AhdI GACNNNNNGTC 1 cut(s) 814
AjnI CCWGG 1 cut(s) 559
AloI GAACNNNNNNTCC 2 cut(s) 1515, 1547
Alw21I GWGCWC 3 cut(s) 405, 457, 992
Alw26I GTCTC 5 cut(s) 127, 247, 975, 1808, 2224
Alw44I GTGCAC 1 cut(s) 453
AlwI GGATC 5 cut(s) 136, 185, 198, 829, 1475
Ama87I CYCGRG 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 818
AoxI GGCC 2 cut(s) 114, 907
ApaLI GTGCAC 1 cut(s) 453
ApoI RAATTY 6 cut(s) 842, 848, 1660, 1694, 1765, 2287
ArsI GACNNNNNNTTYG 2 cut(s) 2607, 2639
AseI ATTAAT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 1161
Asp718I GGTACC 1 cut(s) 715
AspA2I CCTAGG 1 cut(s) 1234
AspS9I GGNCC 3 cut(s) 182, 816, 908
AsuHPI GGTGA 3 cut(s) 791, 2305, 2441
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 2 cut(s) 182, 816
AvrII CCTAGG 1 cut(s) 1234
AxyI CCTNAGG 1 cut(s) 1209
BaeGI GKGCMC 1 cut(s) 457
BamHI GGATCC 1 cut(s) 190
BanI GGYRCC 1 cut(s) 715
BanII GRGCYC 4 cut(s) 405, 992, 1784, 1847
BarI GAAGNNNNNNTAC 2 cut(s) 278, 310
BbsI GAAGAC 2 cut(s) 518, 2038
Bbv12I GWGCWC 3 cut(s) 405, 457, 992
BccI CCATC 6 cut(s) 145, 173, 1147, 1480, 1592, 2203
BceAI ACGGC 1 cut(s) 1831
BcgI CGANNNNNNTGC 2 cut(s) 2386, 2420
BciT130I CCWGG 1 cut(s) 561
BclI TGATCA 1 cut(s) 1506
BcoDI GTCTC 5 cut(s) 127, 247, 975, 1808, 2224
BfaI CTAG 9 cut(s) 122, 1235, 1307, 1361, 1652, 1830, 2028, 2099, 2300
BfmI CTRYAG 2 cut(s) 432, 2752
BfrI CTTAAG 2 cut(s) 2165, 2219
BfuAI ACCTGC 1 cut(s) 437
BglII AGATCT 2 cut(s) 1906, 2503
BlnI CCTAGG 1 cut(s) 1234
BmcAI AGTACT 1 cut(s) 1140
Bme1390I CCNGG 1 cut(s) 561
Bme18I GGWCC 2 cut(s) 182, 816
BmeRI GACNNNNNGTC 1 cut(s) 814
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 3 cut(s) 182, 816, 908
BmiI GGNNCC 3 cut(s) 192, 717, 1783
BmrFI CCNGG 1 cut(s) 561
BmrI ACTGGG 2 cut(s) 1787, 1841
BmsI GCATC 7 cut(s) 115, 1177, 1713, 1827, 2384, 2533, 2601
BmuI ACTGGG 2 cut(s) 1787, 1841
BpiI GAAGAC 2 cut(s) 518, 2038
BpmI CTGGAG 2 cut(s) 543, 692
Bpu10I CCTNAGC 1 cut(s) 666
BpuEI CTTGAG 3 cut(s) 296, 350, 2528
Bsa29I ATCGAT 1 cut(s) 2135
BsaBI GATNNNNATC 1 cut(s) 826
BsaHI GRCGYC 3 cut(s) 612, 2035, 2106
BsaI GGTCTC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 1191, 1234
BsaWI WCCGGW 2 cut(s) 175, 818
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 905, 1542
Bse118I RCCGGY 1 cut(s) 2037
Bse1I ACTGG 6 cut(s) 194, 743, 1053, 1461, 1782, 1847
Bse21I CCTNAGG 1 cut(s) 1209
Bse3DI GCAATG 4 cut(s) 391, 954, 1326, 2064
Bse8I GATNNNNATC 1 cut(s) 826
BseAI TCCGGA 1 cut(s) 818
BseBI CCWGG 1 cut(s) 561
BseCI ATCGAT 1 cut(s) 2135
BseDI CCNNGG 2 cut(s) 1191, 1234
BseGI GGATG 5 cut(s) 184, 535, 572, 811, 2548
BseJI GATNNNNATC 1 cut(s) 826
BseLI CCNNNNNNNGG 3 cut(s) 151, 905, 1542
BseMI GCAATG 4 cut(s) 391, 954, 1326, 2064
BseMII CTCAG 8 cut(s) 332, 882, 1462, 1623, 1810, 1819, 2161, 2219
BseNI ACTGG 6 cut(s) 194, 743, 1053, 1461, 1782, 1847
BseRI GAGGAG 1 cut(s) 2423
BseSI GKGCMC 1 cut(s) 457
BseYI CCCAGC 1 cut(s) 1749
BsgI GTGCAG 1 cut(s) 252
Bsh1285I CGRYCG 2 cut(s) 176, 2182
BshFI GGCC 2 cut(s) 116, 909
BshNI GGYRCC 1 cut(s) 715
BshVI ATCGAT 1 cut(s) 2135
BsiEI CGRYCG 2 cut(s) 176, 2182
BsiHKAI GWGCWC 3 cut(s) 405, 457, 992
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 5 cut(s) 176, 819, 2038, 2201, 2345
BslFI GGGAC 1 cut(s) 2609
BslI CCNNNNNNNGG 3 cut(s) 151, 905, 1542
BsmAI GTCTC 5 cut(s) 127, 247, 975, 1808, 2224
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 2609
BsmI GAATGC 2 cut(s) 971, 1713
BsnI GGCC 2 cut(s) 116, 909
Bso31I GGTCTC 1 cut(s) 127
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 5 cut(s) 405, 457, 992, 1784, 1847
Bsp13I TCCGGA 1 cut(s) 818
Bsp1407I TGTACA 1 cut(s) 1396
BspACI CCGC 4 cut(s) 5, 1292, 1384, 2602
BspANI GGCC 2 cut(s) 116, 909
BspCNI CTCAG 8 cut(s) 333, 883, 1461, 1622, 1809, 1818, 2160, 2220
BspDI ATCGAT 1 cut(s) 2135
BspEI TCCGGA 1 cut(s) 818
BspLI GGNNCC 3 cut(s) 192, 717, 1783
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 1 cut(s) 437
BspPI GGATC 5 cut(s) 136, 185, 198, 829, 1475
BspQI GCTCTTC 1 cut(s) 1371
BspT107I GGYRCC 1 cut(s) 715
BspTI CTTAAG 2 cut(s) 2165, 2219
BspTNI GGTCTC 1 cut(s) 127
BsrDI GCAATG 4 cut(s) 391, 954, 1326, 2064
BsrFI RCCGGY 1 cut(s) 2037
BsrGI TGTACA 1 cut(s) 1396
BsrI ACTGG 6 cut(s) 194, 743, 1053, 1461, 1782, 1847
BssAI RCCGGY 1 cut(s) 2037
BssECI CCNNGG 2 cut(s) 1191, 1234
BssNI GRCGYC 3 cut(s) 612, 2035, 2106
BssT1I CCWWGG 2 cut(s) 1191, 1234
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 7 cut(s) 48, 471, 652, 1034, 1060, 1148, 1854
Bst6I CTCTTC 3 cut(s) 1308, 1371, 2432
BstACI GRCGYC 3 cut(s) 612, 2035, 2106
BstAFI CTTAAG 2 cut(s) 2165, 2219
BstAPI GCANNNNNTGC 2 cut(s) 452, 1444
BstAUI TGTACA 1 cut(s) 1396
BstC8I GCNNGC 3 cut(s) 392, 1576, 1997
BstF5I GGATG 5 cut(s) 184, 535, 572, 811, 2548
BstMAI GTCTC 5 cut(s) 127, 247, 975, 1808, 2224
BstMCI CGRYCG 2 cut(s) 176, 2182
BstMWI GCNNNNNNNGC 8 cut(s) 313, 400, 452, 1176, 1444, 2518, 2708, 2717
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 3 cut(s) 394, 1890, 2389
BstSCI CCNGG 1 cut(s) 559
BstSFI CTRYAG 2 cut(s) 432, 2752
BstSLI GKGCMC 1 cut(s) 457
BstV2I GAAGAC 2 cut(s) 518, 2038
BstX2I RGATCY 3 cut(s) 190, 1906, 2503
BstXI CCANNNNNNTGG 1 cut(s) 750
BstYI RGATCY 3 cut(s) 190, 1906, 2503
Bsu15I ATCGAT 1 cut(s) 2135
Bsu36I CCTNAGG 1 cut(s) 1209
BsuRI GGCC 2 cut(s) 116, 909
BsuTUI ATCGAT 1 cut(s) 2135
BtsCI GGATG 5 cut(s) 184, 535, 572, 811, 2548
BtsI GCAGTG 2 cut(s) 539, 2736
BtsIMutI CAGTG 9 cut(s) 497, 539, 648, 750, 1030, 1046, 1065, 1617, 2736
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 3 cut(s) 392, 1576, 1997
Cfr10I RCCGGY 1 cut(s) 2037
Cfr13I GGNCC 3 cut(s) 182, 816, 908
ClaI ATCGAT 1 cut(s) 2135
CseI GACGC 2 cut(s) 2043, 2104
Csp6I GTAC 8 cut(s) 155, 289, 295, 716, 1139, 1397, 1850, 2325
CviAII CATG 7 cut(s) 391, 398, 1323, 1439, 1627, 1887, 2386
CviQI GTAC 8 cut(s) 155, 289, 295, 716, 1139, 1397, 1850, 2325
DrdI GACNNNNNNGTC 2 cut(s) 180, 886
DriI GACNNNNNGTC 1 cut(s) 814
DseDI GACNNNNNNGTC 2 cut(s) 180, 886
Eam1104I CTCTTC 3 cut(s) 1308, 1371, 2432
Eam1105I GACNNNNNGTC 1 cut(s) 814
EarI CTCTTC 3 cut(s) 1308, 1371, 2432
EciI GGCGGA 1 cut(s) 1307
Ecl136II GAGCTC 2 cut(s) 403, 990
Eco130I CCWWGG 2 cut(s) 1191, 1234
Eco24I GRGCYC 4 cut(s) 405, 992, 1784, 1847
Eco31I GGTCTC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 182, 816
Eco53kI GAGCTC 2 cut(s) 403, 990
Eco57I CTGAAG 4 cut(s) 521, 1119, 1961, 2079
Eco81I CCTNAGG 1 cut(s) 1209
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 2 cut(s) 403, 990
EcoRI GAATTC 1 cut(s) 1660
EcoRII CCWGG 1 cut(s) 559
EcoT14I CCWWGG 2 cut(s) 1191, 1234
EcoT22I ATGCAT 4 cut(s) 1328, 1713, 2399, 2476
EcoT38I GRGCYC 4 cut(s) 405, 992, 1784, 1847
ErhI CCWWGG 2 cut(s) 1191, 1234
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 7 cut(s) 394, 401, 1326, 1442, 1630, 1890, 2389
FalI AAGNNNNNCTT 4 cut(s) 1951, 1983, 2360, 2392
FaqI GGGAC 1 cut(s) 2609
FatI CATG 7 cut(s) 390, 397, 1322, 1438, 1626, 1886, 2385
FauI CCCGC 1 cut(s) 2595
FbaI TGATCA 1 cut(s) 1506
FokI GGATG 5 cut(s) 191, 542, 579, 818, 2555
FriOI GRGCYC 4 cut(s) 405, 992, 1784, 1847
FspBI CTAG 9 cut(s) 122, 1235, 1307, 1361, 1652, 1830, 2028, 2099, 2300
GsaI CCCAGC 1 cut(s) 1753
GsuI CTGGAG 2 cut(s) 543, 692
HaeIII GGCC 2 cut(s) 116, 909
HapII CCGG 5 cut(s) 176, 819, 2038, 2201, 2345
HgaI GACGC 2 cut(s) 2043, 2104
Hin1I GRCGYC 3 cut(s) 612, 2035, 2106
Hin1II CATG 7 cut(s) 394, 401, 1326, 1442, 1630, 1890, 2389
HincII GTYRAC 2 cut(s) 1085, 2094
HindII GTYRAC 2 cut(s) 1085, 2094
HindIII AAGCTT 1 cut(s) 305
HpaII CCGG 5 cut(s) 176, 819, 2038, 2201, 2345
HphI GGTGA 3 cut(s) 791, 2305, 2441
Hpy166II GTNNAC 5 cut(s) 297, 347, 455, 1085, 2094
Hpy8I GTNNAC 5 cut(s) 297, 347, 455, 1085, 2094
Hpy99I CGWCG 3 cut(s) 2108, 2111, 2600
HpyAV CCTTC 7 cut(s) 221, 496, 770, 921, 1936, 2597, 2600
HpyCH4III ACNGT 7 cut(s) 48, 471, 652, 1034, 1060, 1148, 1854
HpyCH4IV ACGT 9 cut(s) 65, 160, 612, 810, 1097, 1425, 1477, 1676, 2106
HpyF10VI GCNNNNNNNGC 8 cut(s) 313, 400, 452, 1176, 1444, 2518, 2708, 2717
HpySE526I ACGT 9 cut(s) 65, 160, 612, 810, 1097, 1425, 1477, 1676, 2106
Hsp92I GRCGYC 3 cut(s) 612, 2035, 2106
Hsp92II CATG 7 cut(s) 394, 401, 1326, 1442, 1630, 1890, 2389
Kpn2I TCCGGA 1 cut(s) 818
KpnI GGTACC 1 cut(s) 719
Ksp22I TGATCA 1 cut(s) 1506
LguI GCTCTTC 1 cut(s) 1371
LmnI GCTCC 4 cut(s) 562, 673, 1489, 1787
LweI GCATC 7 cut(s) 115, 1177, 1713, 1827, 2384, 2533, 2601
MaeI CTAG 9 cut(s) 122, 1235, 1307, 1361, 1652, 1830, 2028, 2099, 2300
MaeII ACGT 9 cut(s) 65, 160, 612, 810, 1097, 1425, 1477, 1676, 2106
MaeIII GTNAC 5 cut(s) 42, 370, 471, 877, 1267
MfeI CAATTG 1 cut(s) 2280
MflI RGATCY 3 cut(s) 190, 1906, 2503
MhlI GDGCHC 5 cut(s) 405, 457, 992, 1784, 1847
MlyI GAGTC 3 cut(s) 1810, 2320, 2357
MmeI TCCRAC 5 cut(s) 270, 463, 783, 949, 1368
Mph1103I ATGCAT 4 cut(s) 1328, 1713, 2399, 2476
MroI TCCGGA 1 cut(s) 818
MroXI GAANNNNTTC 1 cut(s) 1161
MseI TTAA 8 cut(s) 218, 525, 2009, 2156, 2166, 2220, 2372, 2444
MslI CAYNNNNRTG 5 cut(s) 1035, 1283, 2427, 2554, 2597
MspA1I CMGCKG 3 cut(s) 340, 1749, 1999
MspCI CTTAAG 2 cut(s) 2165, 2219
MspI CCGG 5 cut(s) 176, 819, 2038, 2201, 2345
MspR9I CCNGG 1 cut(s) 561
MunI CAATTG 1 cut(s) 2280
Mva1269I GAATGC 2 cut(s) 971, 1713
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 8 cut(s) 313, 400, 452, 1176, 1444, 2518, 2708, 2717
NlaIII CATG 7 cut(s) 394, 401, 1326, 1442, 1630, 1890, 2389
NlaIV GGNNCC 3 cut(s) 192, 717, 1783
NmuCI GTSAC 2 cut(s) 471, 877
NsiI ATGCAT 4 cut(s) 1328, 1713, 2399, 2476
NspI RCATGY 3 cut(s) 394, 1890, 2389
PaeI GCATGC 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 38
PciI ACATGT 1 cut(s) 2385
PciSI GCTCTTC 1 cut(s) 1371
PctI GAATGC 2 cut(s) 971, 1713
PdmI GAANNNNTTC 1 cut(s) 1161
PflFI GACNNNGTC 1 cut(s) 2105
PflMI CCANNNNNTGG 2 cut(s) 151, 1542
PfoI TCCNGGA 1 cut(s) 559
Ple19I CGATCG 1 cut(s) 2182
PleI GAGTC 3 cut(s) 1809, 2319, 2356
PpsI GAGTC 3 cut(s) 1809, 2319, 2356
PscI ACATGT 1 cut(s) 2385
PshBI ATTAAT 1 cut(s) 218
PsiI TTATAA 2 cut(s) 711, 2175
Psp124BI GAGCTC 2 cut(s) 405, 992
Psp1406I AACGTT 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 1749
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 3 cut(s) 192, 717, 1783
PspPI GGNCC 3 cut(s) 182, 816, 908
PspXI VCTCGAGB 1 cut(s) 38
PstI CTGCAG 1 cut(s) 436
PsuI RGATCY 3 cut(s) 190, 1906, 2503
PsyI GACNNNGTC 1 cut(s) 2105
PvuI CGATCG 1 cut(s) 2182
PvuII CAGCTG 3 cut(s) 340, 1749, 1999
RsaI GTAC 8 cut(s) 156, 290, 296, 717, 1140, 1398, 1851, 2326
RsaNI GTAC 8 cut(s) 155, 289, 295, 716, 1139, 1397, 1850, 2325
RseI CAYNNNNRTG 5 cut(s) 1035, 1283, 2427, 2554, 2597
SacI GAGCTC 2 cut(s) 405, 992
SapI GCTCTTC 1 cut(s) 1371
SaqAI TTAA 8 cut(s) 218, 525, 2009, 2156, 2166, 2220, 2372, 2444
Sau96I GGNCC 3 cut(s) 182, 816, 908
ScaI AGTACT 1 cut(s) 1140
SchI GAGTC 3 cut(s) 1810, 2320, 2357
ScrFI CCNGG 1 cut(s) 561
SduI GDGCHC 5 cut(s) 405, 457, 992, 1784, 1847
SfaNI GCATC 7 cut(s) 115, 1177, 1713, 1827, 2384, 2533, 2601
SfcI CTRYAG 2 cut(s) 432, 2752
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 2 cut(s) 182, 816
SlaI CTCGAG 1 cut(s) 38
SmiMI CAYNNNNRTG 5 cut(s) 1035, 1283, 2427, 2554, 2597
SmlI CTYRAG 6 cut(s) 38, 311, 329, 2165, 2219, 2507
SmoI CTYRAG 6 cut(s) 38, 311, 329, 2165, 2219, 2507
SphI GCATGC 1 cut(s) 394
SsiI CCGC 4 cut(s) 5, 1292, 1384, 2602
SspMI CTAG 9 cut(s) 122, 1235, 1307, 1361, 1652, 1830, 2028, 2099, 2300
SstI GAGCTC 2 cut(s) 405, 992
StyD4I CCNGG 1 cut(s) 559
StyI CCWWGG 2 cut(s) 1191, 1234
TaaI ACNGT 7 cut(s) 48, 471, 652, 1034, 1060, 1148, 1854
TaiI ACGT 9 cut(s) 68, 163, 615, 813, 1100, 1428, 1480, 1679, 2109
TaqII GACCGA 1 cut(s) 151
TatI WGTACW 5 cut(s) 288, 294, 1138, 1396, 1849
Tru1I TTAA 8 cut(s) 218, 525, 2009, 2156, 2166, 2220, 2372, 2444
Tru9I TTAA 8 cut(s) 218, 525, 2009, 2156, 2166, 2220, 2372, 2444
TscAI CASTG 9 cut(s) 504, 546, 655, 750, 1037, 1053, 1065, 1617, 2743
TseFI GTSAC 2 cut(s) 471, 877
Tsp45I GTSAC 2 cut(s) 471, 877
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 9 cut(s) 504, 546, 655, 750, 1037, 1053, 1065, 1617, 2743
Tth111I GACNNNGTC 1 cut(s) 2105
Van91I CCANNNNNTGG 2 cut(s) 151, 1542
Vha464I CTTAAG 2 cut(s) 2165, 2219
VneI GTGCAC 1 cut(s) 453
VpaK11BI GGWCC 2 cut(s) 182, 816
VspI ATTAAT 1 cut(s) 218
XapI RAATTY 6 cut(s) 842, 848, 1660, 1694, 1765, 2287
XbaI TCTAGA 2 cut(s) 1306, 2299
XceI RCATGY 3 cut(s) 394, 1890, 2389
XcmI CCANNNNNNNNNTGG 1 cut(s) 2071
XhoI CTCGAG 1 cut(s) 38
XmaJI CCTAGG 1 cut(s) 1234
XmnI GAANNNNTTC 1 cut(s) 1161
XspI CTAG 9 cut(s) 122, 1235, 1307, 1361, 1652, 1830, 2028, 2099, 2300
ZraI GACGTC 2 cut(s) 613, 2107
ZrmI AGTACT 1 cut(s) 1140
Zsp2I ATGCAT 4 cut(s) 1328, 1713, 2399, 2476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.