Rh3AG236200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000039
Physical Location & Seq
Reverse (-)
24408174 .. 24415912
7739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG236200.1

Sequence Viewer

Length: 2574 bp
ATGCCTTGTCTCTTGGTGGTGTGCTCTTGGGCTTCTGACATGGTGTCATTTTCTGCATTCTCAATGCATTGCAAGAAACAATCCTATATTCACGTCTACTTTGATCTCGCATTATCGATCTCAGCAATGGTGATAATCTGCGGAGCTGGTTTAGCGGTGACATTTTGGGCGCTGTGTGATTCTGTTAAGCAAGTGAAGGACCAGATACTGATATTCAAACCCCAACTCGGGGATATCACGTCCACTCTGGACTCTTTGCAGCCCCTGATCAAGGAGATGGCACAGTGTGAGAAGGAAATGGACGGTCCAGAGGAGGAACTAGAGAATCTTAGAGTGGAAATGGAGGAAGGAGTGGAGCTTATTCGCAAGTGTTCGGAAGCGAGCAGATTCAAGAAGTATAAGTATGGCAGCAGACTTGTTGAATTGAGCAAGTCACTTCAGAGGCTGTTGGAGATATTGAAAGTGCAGGGCATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGGAACATTGAAAATGTGGTGCACAGAATTGAAGGGAATTGTGTGATACAGAACAATCAGTCTGAAAAAATTGAAGGGTGGTGTGCTGTGCCTGAGCCTCCAACAATTACGGTTGGATTGGATTTGCCTTTGAGGGAATTGAAGATGAAGCTCTTTAAGGACGGAATGTCGATGGTGATACTTACTGCTCCAGGAGGATGTGGGAAAACTACACTGGCAACAAAATTATGTCATGATGCAGAAGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACTTATACCTAATTGTAAAAGAGCTATATCATCGAACGGGTGCCCAGGTCCCCGATTTCCAAAGTGAAGTAACTGCAGTTAGCTGGCTGCAGCAATTTCTGAAGCTAACAGGACAAAATCCTATGTTGTTGATCCTGGATGATGTTTGGGCTGGATCAGAATTCATTCTTGAGAAGTTCGATGAATTAAAATTGACAAATTACAAGATATTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTATTTAGAATGTTTGAACCGCAAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGAGACAGGAGCTCTTATATTTCAGAAGATCTTTCAACAAAGATAGTAGAACGTTGTATGGGATTTCCACTTGCAATTACAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTAATGGAATGGTCTAGAGGTTCTTCTGTTCTTGAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGTAGTTTAGATGTTTTGTATGCAGAGAAGCCTCTTATGAGGGAATGCTTTATAGACCTTGGTTTATTTCCTGAAGATCAAAGAATTCCTACCACTGTCCTGATTGATATGTGGGCAGAGTTATATGGCATACATGAAGACATTTCGTCCATTGCGAACCTCTATGAACTTGCCTCTCGAAGTTTGGCTAATCTGGTAGTCACAAGGAAGGACAACAAGGATAGTGATGGCTATTACACTGAACACTTCGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCAGCAGTTGAGTCAGGAACCACTACAACATAGAATGAGGCTTATGATTGACATATGTGGAGACAACCTTCCTATGTGGTGGAAAGAACAAAAGTATCAATCCCTCATTGCTCGCCTTGTATCGATCTCGACAGATGAAGAGTTCTCATCTAAATGGCAAAACTTGCAACTACCAGAAGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTCTGTGGAGAAAATGAATAAATTGAAGACTCTAGTACTCACTAGTTACGGTATCAGTCCTGCTGAGTTGAATAACTTTCAACTAGTTGATTCAAAACCGAATCTGAAGAGAATCAGATTAGAACGAATTTCAATTCCTTCCATAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTAGCATCGGCCAAGGTTTTGGTAATGGTTCCATCCATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGTTGCCTCCCCAATTGTGTGACCTTATTCAATTGAAGAAACTCAGTGTCACCAATTCTCATAAACTCTCTGCCTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGCTGTTGAGGCTCAGGTGTTGTACAGACTTGTCAGAATTGCCCGGCTCGATTAGGAACCTCAAGAAGTTAAATTTGCTTGACATATCTGATTGCTTTAGCATTAAGGAATTGCCTGAAGACATTGGAGAATTGTGCAATTTAGAAAGGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCTTCAGTTTTGAATCTTGAACAGTTGAAGGAAGTGACATGTGATGAAGAGACTGAAATTTTATGGGAACCCTTTTTACCCTTTCTCAAGAACATGCACATGAAGGCTGTGAAAGAAGATATGAACCTGAATTGGCTCCACAAGGCACATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

857

Amino Acids

97.77

Weight (kDa)

5.64

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 45 - 170 1e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 212 - 367 4e-15 NB-ARC domain
AAA_22 PF13401 225 - 326 4.2e-06 AAA domain
LRR_14 PF23598 694 - 773 8.8e-09 Leucine-rich repeat region
LRR_14 PF23598 777 - 826 2.4e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1024
Acc36I ACCTGC 1 cut(s) 1965
AccB1I GGYRCC 1 cut(s) 842
AccB7I CCANNNNNTGG 1 cut(s) 1043
AccI GTMKAC 1 cut(s) 96
AciI CCGC 4 cut(s) 141, 155, 1075, 1218
AclI AACGTT 1 cut(s) 1168
AclWI GGATC 2 cut(s) 928, 964
AcoI YGGCCR 1 cut(s) 2032
AcsI RAATTY 7 cut(s) 758, 962, 1398, 1953, 2224, 2302, 2475
AcuI CTGAAG 7 cut(s) 422, 923, 1407, 1952, 2223, 2367, 2406
AdeI CACNNNGTG 1 cut(s) 287
AfaI GTAC 2 cut(s) 1863, 2254
AfiI CCNNNNNNNGG 6 cut(s) 227, 228, 229, 271, 1043, 1974
AflIII ACRYGT 1 cut(s) 2456
AhdI GACNNNNNGTC 3 cut(s) 43, 670, 1459
AhlI ACTAGT 2 cut(s) 1868, 1909
AjiI CACGTC 2 cut(s) 94, 240
AjnI CCWGG 3 cut(s) 694, 846, 936
AjuI GAANNNNNNNTTGG 4 cut(s) 476, 508, 1030, 1062
AluBI AGCT 8 cut(s) 146, 358, 655, 826, 885, 907, 1128, 2233
AluI AGCT 8 cut(s) 146, 358, 655, 826, 885, 907, 1128, 2233
Alw21I GWGCWC 4 cut(s) 26, 528, 1130, 2399
Alw26I GTCTC 5 cut(s) 14, 1113, 1653, 2383, 2462
Alw44I GTGCAC 1 cut(s) 524
AlwI GGATC 2 cut(s) 928, 964
AlwNI CAGNNNCTG 3 cut(s) 208, 265, 445
Ama87I CYCGRG 2 cut(s) 227, 2398
AoxI GGCC 1 cut(s) 2032
ApaLI GTGCAC 1 cut(s) 524
ApeKI GCWGC 6 cut(s) 259, 408, 889, 892, 1106, 1222
ApoI RAATTY 7 cut(s) 758, 962, 1398, 1953, 2224, 2302, 2475
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 3 cut(s) 199, 305, 850
AsuC2I CCSGG 1 cut(s) 2274
AsuHPI GGTGA 7 cut(s) 142, 169, 514, 691, 1027, 1091, 2164
AvaI CYCGRG 2 cut(s) 227, 2398
AvaII GGWCC 3 cut(s) 199, 305, 850
AxyI CCTNAGG 1 cut(s) 1114
BaeGI GKGCMC 2 cut(s) 528, 847
BaeI ACNNNNGTAYC 2 cut(s) 1676, 1709
BanI GGYRCC 1 cut(s) 842
BanII GRGCYC 1 cut(s) 1130
BbsI GAAGAC 5 cut(s) 766, 1458, 1859, 2211, 2355
Bbv12I GWGCWC 4 cut(s) 26, 528, 1130, 2399
BbvI GCAGC 6 cut(s) 271, 420, 876, 904, 1118, 1234
BccI CCATC 4 cut(s) 271, 670, 1535, 2063
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 3 cut(s) 696, 848, 938
BciVI GTATCC 1 cut(s) 1989
BclI TGATCA 1 cut(s) 267
BcnI CCSGG 1 cut(s) 2274
BcoDI GTCTC 5 cut(s) 14, 1113, 1653, 2383, 2462
BcuI ACTAGT 2 cut(s) 1868, 1909
BfaI CTAG 6 cut(s) 320, 1263, 1859, 1869, 1910, 2219
BfmI CTRYAG 2 cut(s) 876, 890
BfoI RGCGCY 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 1965
BfuI GTATCC 1 cut(s) 1989
BglII AGATCT 1 cut(s) 1144
BisI GCNGC 6 cut(s) 260, 409, 890, 893, 1107, 1223
BlsI GCNGC 6 cut(s) 261, 410, 891, 894, 1108, 1224
BmcAI AGTACT 1 cut(s) 1863
Bme1390I CCNGG 4 cut(s) 696, 848, 938, 2274
Bme18I GGWCC 3 cut(s) 199, 305, 850
BmeRI GACNNNNNGTC 3 cut(s) 43, 670, 1459
BmeT110I CYCGRG 2 cut(s) 227, 2398
BmgBI CACGTC 2 cut(s) 94, 240
BmgT120I GGNCC 3 cut(s) 199, 305, 850
BmiI GGNNCC 7 cut(s) 844, 852, 1617, 2053, 2288, 2487, 2555
BmrFI CCNGG 4 cut(s) 696, 848, 938, 2274
BmsI GCATC 4 cut(s) 730, 1072, 1118, 2037
BpiI GAAGAC 5 cut(s) 766, 1458, 1859, 2211, 2355
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 3 cut(s) 597, 1583, 2243
BpuEI CTTGAG 4 cut(s) 992, 1301, 2276, 2489
BpuMI CCSGG 1 cut(s) 2274
Bsa29I ATCGAT 2 cut(s) 116, 1721
BsaJI CCNNGG 3 cut(s) 846, 1372, 2035
Bsc4I CCNNNNNNNGG 6 cut(s) 227, 228, 229, 271, 1043, 1974
Bse1I ACTGG 1 cut(s) 723
Bse21I CCTNAGG 1 cut(s) 1114
Bse3DI GCAATG 8 cut(s) 67, 132, 775, 1092, 1464, 1704, 2116, 2566
BseBI CCWGG 3 cut(s) 696, 848, 938
BseCI ATCGAT 2 cut(s) 116, 1721
BseDI CCNNGG 3 cut(s) 846, 1372, 2035
BseGI GGATG 5 cut(s) 484, 707, 946, 1109, 2055
BseLI CCNNNNNNNGG 6 cut(s) 227, 228, 229, 271, 1043, 1974
BseMI GCAATG 8 cut(s) 67, 132, 775, 1092, 1464, 1704, 2116, 2566
BseMII CTCAG 6 cut(s) 135, 588, 1815, 1881, 2179, 2257
BseNI ACTGG 1 cut(s) 723
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 2 cut(s) 528, 847
BseXI GCAGC 6 cut(s) 271, 420, 876, 904, 1118, 1234
BsgI GTGCAG 1 cut(s) 485
BshFI GGCC 1 cut(s) 2034
BshNI GGYRCC 1 cut(s) 842
BshVI ATCGAT 2 cut(s) 116, 1721
BsiHKAI GWGCWC 4 cut(s) 26, 528, 1130, 2399
BsiHKCI CYCGRG 2 cut(s) 227, 2398
BsiSI CCGG 1 cut(s) 2274
BslFI GGGAC 1 cut(s) 836
BslI CCNNNNNNNGG 6 cut(s) 227, 228, 229, 271, 1043, 1974
BsmAI GTCTC 5 cut(s) 14, 1113, 1653, 2383, 2462
BsmFI GGGAC 1 cut(s) 836
BsmI GAATGC 2 cut(s) 56, 1364
BsnI GGCC 1 cut(s) 2034
BsoBI CYCGRG 2 cut(s) 227, 2398
Bsp1286I GDGCHC 5 cut(s) 26, 528, 847, 1130, 2399
Bsp1407I TGTACA 1 cut(s) 2252
Bsp143I GATC 9 cut(s) 103, 117, 267, 933, 956, 1144, 1208, 1390, 1722
BspACI CCGC 4 cut(s) 141, 155, 1075, 1218
BspANI GGCC 1 cut(s) 2034
BspCNI CTCAG 6 cut(s) 134, 589, 1816, 1882, 2178, 2256
BspDI ATCGAT 2 cut(s) 116, 1721
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 7 cut(s) 844, 852, 1617, 2053, 2288, 2487, 2555
BspMAI CTGCAG 2 cut(s) 880, 894
BspMI ACCTGC 1 cut(s) 1965
BspPI GGATC 2 cut(s) 928, 964
BspT107I GGYRCC 1 cut(s) 842
BsrDI GCAATG 8 cut(s) 67, 132, 775, 1092, 1464, 1704, 2116, 2566
BsrGI TGTACA 1 cut(s) 2252
BsrI ACTGG 1 cut(s) 723
BssECI CCNNGG 3 cut(s) 846, 1372, 2035
BssMI GATC 9 cut(s) 103, 117, 267, 933, 956, 1144, 1208, 1390, 1722
BssT1I CCWWGG 2 cut(s) 1372, 2035
Bst2UI CCWGG 3 cut(s) 696, 848, 938
Bst6I CTCTTC 3 cut(s) 1731, 1928, 2460
BstAPI GCANNNNNTGC 2 cut(s) 1579, 1762
BstAUI TGTACA 1 cut(s) 2252
BstC8I GCNNGC 4 cut(s) 382, 887, 1220, 1711
BstDEI CTNAG 9 cut(s) 121, 329, 597, 1114, 1583, 1824, 1890, 2165, 2243
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 5 cut(s) 484, 707, 946, 1109, 2055
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 9 cut(s) 106, 120, 270, 936, 959, 1147, 1211, 1393, 1725
BstMAI GTCTC 5 cut(s) 14, 1113, 1653, 2383, 2462
BstMBI GATC 9 cut(s) 103, 117, 267, 933, 956, 1144, 1208, 1390, 1722
BstMWI GCNNNNNNNGC 4 cut(s) 152, 1579, 1762, 2239
BstNI CCWGG 3 cut(s) 696, 848, 938
BstNSI RCATGY 3 cut(s) 1582, 2460, 2515
BstSCI CCNGG 4 cut(s) 694, 846, 936, 2272
BstSFI CTRYAG 2 cut(s) 876, 890
BstSLI GKGCMC 2 cut(s) 528, 847
BstV1I GCAGC 6 cut(s) 271, 420, 876, 904, 1118, 1234
BstV2I GAAGAC 5 cut(s) 766, 1458, 1859, 2211, 2355
BstX2I RGATCY 1 cut(s) 1144
BstXI CCANNNNNNTGG 1 cut(s) 2042
BstYI RGATCY 1 cut(s) 1144
Bsu15I ATCGAT 2 cut(s) 116, 1721
Bsu36I CCTNAGG 1 cut(s) 1114
BsuI GTATCC 1 cut(s) 1989
BsuRI GGCC 1 cut(s) 2034
BsuTUI ATCGAT 2 cut(s) 116, 1721
BtrI CACGTC 2 cut(s) 94, 240
BtsCI GGATG 5 cut(s) 484, 707, 946, 1109, 2055
BtsIMutI CAGTG 6 cut(s) 290, 716, 1407, 1551, 2173, 2399
BveI ACCTGC 1 cut(s) 1965
Cac8I GCNNGC 4 cut(s) 382, 887, 1220, 1711
CaiI CAGNNNCTG 3 cut(s) 208, 265, 445
CciI TCATGA 1 cut(s) 736
CfoI GCGC 1 cut(s) 172
Cfr13I GGNCC 3 cut(s) 199, 305, 850
ClaI ATCGAT 2 cut(s) 116, 1721
Csp6I GTAC 2 cut(s) 1862, 2253
CviQI GTAC 2 cut(s) 1862, 2253
DdeI CTNAG 9 cut(s) 121, 329, 597, 1114, 1583, 1824, 1890, 2165, 2243
DpnI GATC 9 cut(s) 105, 119, 269, 935, 958, 1146, 1210, 1392, 1724
DpnII GATC 9 cut(s) 103, 117, 267, 933, 956, 1144, 1208, 1390, 1722
DraIII CACNNNGTG 1 cut(s) 287
DrdI GACNNNNNNGTC 1 cut(s) 1024
DriI GACNNNNNGTC 3 cut(s) 43, 670, 1459
DseDI GACNNNNNNGTC 1 cut(s) 1024
EaeI YGGCCR 1 cut(s) 2032
Eam1104I CTCTTC 3 cut(s) 1731, 1928, 2460
Eam1105I GACNNNNNGTC 3 cut(s) 43, 670, 1459
EarI CTCTTC 3 cut(s) 1731, 1928, 2460
Ecl136II GAGCTC 1 cut(s) 1128
Eco130I CCWWGG 2 cut(s) 1372, 2035
Eco24I GRGCYC 1 cut(s) 1130
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 3 cut(s) 199, 305, 850
Eco53kI GAGCTC 1 cut(s) 1128
Eco57I CTGAAG 7 cut(s) 422, 923, 1407, 1952, 2223, 2367, 2406
Eco81I CCTNAGG 1 cut(s) 1114
Eco88I CYCGRG 2 cut(s) 227, 2398
EcoICRI GAGCTC 1 cut(s) 1128
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 850
EcoRI GAATTC 2 cut(s) 962, 1398
EcoRII CCWGG 3 cut(s) 694, 846, 936
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 1372, 2035
EcoT22I ATGCAT 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 1130
ErhI CCWWGG 2 cut(s) 1372, 2035
FalI AAGNNNNNCTT 4 cut(s) 788, 820, 1015, 1047
FaqI GGGAC 1 cut(s) 836
FauI CCCGC 1 cut(s) 1211
FauNDI CATATG 1 cut(s) 1652
FbaI TGATCA 1 cut(s) 267
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 6 cut(s) 260, 409, 890, 893, 1107, 1223
FokI GGATG 5 cut(s) 491, 714, 953, 1096, 2042
FriOI GRGCYC 1 cut(s) 1130
Fsp4HI GCNGC 6 cut(s) 260, 409, 890, 893, 1107, 1223
FspBI CTAG 6 cut(s) 320, 1263, 1859, 1869, 1910, 2219
GlaI GCGC 1 cut(s) 171
GluI GCNGC 6 cut(s) 260, 409, 890, 893, 1107, 1223
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 173
HaeIII GGCC 1 cut(s) 2034
HapII CCGG 1 cut(s) 2274
HhaI GCGC 1 cut(s) 172
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HpaII CCGG 1 cut(s) 2274
HphI GGTGA 7 cut(s) 142, 169, 514, 691, 1027, 1091, 2164
Hpy166II GTNNAC 3 cut(s) 97, 243, 526
Hpy8I GTNNAC 3 cut(s) 97, 243, 526
HpyCH4IV ACGT 3 cut(s) 93, 239, 1168
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 1579, 1762, 2239
HpyF3I CTNAG 9 cut(s) 121, 329, 597, 1114, 1583, 1824, 1890, 2165, 2243
HpySE526I ACGT 3 cut(s) 93, 239, 1168
HspAI GCGC 1 cut(s) 170
Ksp22I TGATCA 1 cut(s) 267
Kzo9I GATC 9 cut(s) 103, 117, 267, 933, 956, 1144, 1208, 1390, 1722
LmnI GCTCC 5 cut(s) 143, 355, 697, 1125, 2559
Lsp1109I GCAGC 6 cut(s) 271, 420, 876, 904, 1118, 1234
LweI GCATC 4 cut(s) 730, 1072, 1118, 2037
MaeI CTAG 6 cut(s) 320, 1263, 1859, 1869, 1910, 2219
MaeII ACGT 3 cut(s) 93, 239, 1168
MalI GATC 9 cut(s) 105, 119, 269, 935, 958, 1146, 1210, 1392, 1724
MboI GATC 9 cut(s) 103, 117, 267, 933, 956, 1144, 1208, 1390, 1722
MfeI CAATTG 2 cut(s) 2135, 2153
MflI RGATCY 1 cut(s) 1144
MhlI GDGCHC 5 cut(s) 26, 528, 847, 1130, 2399
MlyI GAGTC 5 cut(s) 245, 1292, 1618, 1835, 1849
MmeI TCCRAC 4 cut(s) 429, 598, 629, 1183
Mph1103I ATGCAT 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 966
MseI TTAA 5 cut(s) 186, 660, 989, 2300, 2334
MslI CAYNNNNRTG 2 cut(s) 1750, 2516
MspI CCGG 1 cut(s) 2274
MspR9I CCNGG 4 cut(s) 696, 848, 938, 2274
MunI CAATTG 2 cut(s) 2135, 2153
Mva1269I GAATGC 2 cut(s) 56, 1364
MvaI CCWGG 3 cut(s) 696, 848, 938
MwoI GCNNNNNNNGC 4 cut(s) 152, 1579, 1762, 2239
NciI CCSGG 1 cut(s) 2274
NdeI CATATG 1 cut(s) 1652
NdeII GATC 9 cut(s) 103, 117, 267, 933, 956, 1144, 1208, 1390, 1722
NlaIV GGNNCC 7 cut(s) 844, 852, 1617, 2053, 2288, 2487, 2555
NmuCI GTSAC 7 cut(s) 157, 432, 1015, 1513, 2141, 2170, 2452
NsiI ATGCAT 1 cut(s) 69
NspI RCATGY 3 cut(s) 1582, 2460, 2515
PaeR7I CTCGAG 1 cut(s) 2398
PagI TCATGA 1 cut(s) 736
PciI ACATGT 1 cut(s) 2456
PcsI WCGNNNNNNNCGW 1 cut(s) 1466
PctI GAATGC 2 cut(s) 56, 1364
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 7 cut(s) 179, 325, 387, 1916, 1927, 1938, 2431
PflMI CCANNNNNTGG 1 cut(s) 1043
PfoI TCCNGGA 2 cut(s) 694, 936
PkrI GCNGC 6 cut(s) 261, 410, 891, 894, 1108, 1224
PleI GAGTC 5 cut(s) 245, 1291, 1617, 1834, 1849
PpsI GAGTC 5 cut(s) 245, 1291, 1617, 1834, 1849
PpuMI RGGWCCY 1 cut(s) 850
PscI ACATGT 1 cut(s) 2456
Psp124BI GAGCTC 1 cut(s) 1130
Psp1406I AACGTT 1 cut(s) 1168
Psp5II RGGWCCY 1 cut(s) 850
Psp6I CCWGG 3 cut(s) 694, 846, 936
PspGI CCWGG 3 cut(s) 694, 846, 936
PspN4I GGNNCC 7 cut(s) 844, 852, 1617, 2053, 2288, 2487, 2555
PspPI GGNCC 3 cut(s) 199, 305, 850
PspPPI RGGWCCY 1 cut(s) 850
PsrI GAACNNNNNNTAC 2 cut(s) 2478, 2510
PstI CTGCAG 2 cut(s) 880, 894
PstNI CAGNNNCTG 3 cut(s) 208, 265, 445
PsuI RGATCY 1 cut(s) 1144
RsaI GTAC 2 cut(s) 1863, 2254
RsaNI GTAC 2 cut(s) 1862, 2253
RseI CAYNNNNRTG 2 cut(s) 1750, 2516
SacI GAGCTC 1 cut(s) 1130
SaqAI TTAA 5 cut(s) 186, 660, 989, 2300, 2334
SatI GCNGC 6 cut(s) 260, 409, 890, 893, 1107, 1223
Sau3AI GATC 9 cut(s) 103, 117, 267, 933, 956, 1144, 1208, 1390, 1722
Sau96I GGNCC 3 cut(s) 199, 305, 850
ScaI AGTACT 1 cut(s) 1863
SchI GAGTC 5 cut(s) 245, 1292, 1618, 1835, 1849
ScrFI CCNGG 4 cut(s) 696, 848, 938, 2274
SduI GDGCHC 5 cut(s) 26, 528, 847, 1130, 2399
SfaNI GCATC 4 cut(s) 730, 1072, 1118, 2037
SfcI CTRYAG 2 cut(s) 876, 890
Sfr274I CTCGAG 1 cut(s) 2398
SinI GGWCC 3 cut(s) 199, 305, 850
SlaI CTCGAG 1 cut(s) 2398
SmiMI CAYNNNNRTG 2 cut(s) 1750, 2516
SmlI CTYRAG 5 cut(s) 971, 1280, 2291, 2398, 2504
SmoI CTYRAG 5 cut(s) 971, 1280, 2291, 2398, 2504
SpeI ACTAGT 2 cut(s) 1868, 1909
SsiI CCGC 4 cut(s) 141, 155, 1075, 1218
SspI AATATT 1 cut(s) 2098
SspMI CTAG 6 cut(s) 320, 1263, 1859, 1869, 1910, 2219
SstI GAGCTC 1 cut(s) 1130
StyD4I CCNGG 4 cut(s) 694, 846, 936, 2272
StyI CCWWGG 2 cut(s) 1372, 2035
TaiI ACGT 3 cut(s) 96, 242, 1171
TaqI TCGA 9 cut(s) 116, 674, 835, 981, 1492, 1721, 1727, 2279, 2399
TatI WGTACW 2 cut(s) 1861, 2252
TfiI GAWTC 7 cut(s) 179, 325, 387, 1916, 1927, 1938, 2431
Tru1I TTAA 5 cut(s) 186, 660, 989, 2300, 2334
Tru9I TTAA 5 cut(s) 186, 660, 989, 2300, 2334
TscAI CASTG 6 cut(s) 290, 723, 1414, 1558, 2173, 2399
TseFI GTSAC 7 cut(s) 157, 432, 1015, 1513, 2141, 2170, 2452
TseI GCWGC 6 cut(s) 259, 408, 889, 892, 1106, 1222
Tsp45I GTSAC 7 cut(s) 157, 432, 1015, 1513, 2141, 2170, 2452
TspGWI ACGGA 1 cut(s) 681
TspRI CASTG 6 cut(s) 290, 723, 1414, 1558, 2173, 2399
Van91I CCANNNNNTGG 1 cut(s) 1043
VneI GTGCAC 1 cut(s) 524
VpaK11BI GGWCC 3 cut(s) 199, 305, 850
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 7 cut(s) 758, 962, 1398, 1953, 2224, 2302, 2475
XbaI TCTAGA 1 cut(s) 1262
XceI RCATGY 3 cut(s) 1582, 2460, 2515
XcmI CCANNNNNNNNNTGG 1 cut(s) 1233
XhoI CTCGAG 1 cut(s) 2398
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 6 cut(s) 320, 1263, 1859, 1869, 1910, 2219
ZrmI AGTACT 1 cut(s) 1863
Zsp2I ATGCAT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.