FvH4_6g50310

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000039
Physical Location & Seq
Forward (+)
37458267 .. 37470424
12158 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50310.t7

Sequence Viewer

Length: 1542 bp
ATGGTTGCATTGCATTGCATCACTGATAATATTGCACACAGCTCTCTCCTCTCTTGTTTTCTAGTTTCTATTGAAGAGAGCTGGTTATTGAAAGCTAGAGTACTAGCTAGTCTGCAACACTACATCTGCATCATGGAAAACTTAGTTCTCGAAAAAGCATTCGAAGTACTGTGTGCTGGAGTTCAGGATCTGATTGTCAAGAATTTCATTTTCAAGTCTCTCCTCAACGACGCCAAATCCAGACTCGACGATTTGGCACCACTGATCAAGAAGCTGGCGGATTATAACCAGGTAATGGGTGGCCAAGAGAAGGAACTGGAAGCTCTAAAGGGAGTCCTGGCAAAGGGCCAACAGCTCGTCGACAAGTGCTCAACCATTACACGATGGAGCCCCTTCAAAAAGTACAAGTACGCCAGACAAATTCTCCAGTGGGAAAAATCTCTGAAAAGCCCGTTGAAGAGTGTGAAGCTGCAAGAAGCAGTGAATGTAATGGACATGAGGATCGATATGCAACGCATGATGCTGAAACAACAACATTCTGGAGAAAGTTCGAGTACTAGAACACGACTTGAACTTCCACCACTTCCACGGCTTATAGTTGGACTGGATGCTCCTATGAAAGACTTGAAGGAGAAGCTACTTTGCGATGATGGGGAGTCAATGCTTGTGCTCACTGCTCCTGGAGGCTGCGGGAAAACTACTTTGGCCACAATGTTTTGTAAAGATGAAGAGGTCAAAGGTAAATTCAAGAACAATATTCTCTTTATTCCTGTATCCCAAAATGGCAATTTGGAACTTGTTGTGAAACGACTTTGTAAATACCTGGGTTCTCCGGTTCGTAGTTTTCAAAACGAAATACATATGCTTCAATGGATCGAAACATTTAGAAAGGAAAGATCAGATCCTCTATTATTGGTCCTCGATGATATTTGGTCTGGACAAGAATCCATTCTTGAGAGCTTTCAATTTGAAATGTCAAATTTCAAGATTTTAGTGACATCAAGATCTGAATTTCCAAGATTTAGTTCTGTATATCAGTTAAAGTTATTGGATGATGACAACGCCATGAAACTCTTTTATCACTCGACATCATTGGGAGATAAGCGCTCTTCTCGTACTATAGAAGATATCTCAAGAAAGATAGTAAAGCGTTGCAACGGATTTCCACTTGCTATTACAGTTGTGGGAAGATCACTTGCTGGGCAGCCTTTAGAGGTTTGGCGAAGAAGAGAGTCGGAATGGTCTAAGGGTTCTTCAATTTTAGATTCTGAGACGAAATTGTTTCTTTGCCTCCAAAGCAGCTTAGATGCTCTTGATAAAGGATTTACAACTGTGAAAGATTGTTACTTAGACCTGGGATCTTTTCCTGAAGACTACAAAATCCCGGTTGGTGTCCTTATTGATATGTGGGCAGAGTTGTATGATCTAGATGATGATATTGTGTTTGCATATGTTCATGAACTATGCAATCGAAATCTGGCTACCCTTGTAGACGAAAGATGGAGAATTTTCAATGAAATGGCCCCAGATTCAACTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

514

Amino Acids

58.56

Weight (kDa)

6.45

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 49 - 176 1e-17 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 204 - 360 8.8e-16 NB-ARC domain
AAA_22 PF13401 218 - 310 9.3e-07 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 256
AccB7I CCANNNNNTGG 1 cut(s) 295
AccI GTMKAC 2 cut(s) 360, 1491
AciI CCGC 2 cut(s) 278, 690
AclWI GGATC 5 cut(s) 195, 509, 881, 896, 1366
AcoI YGGCCR 2 cut(s) 301, 705
AcsI RAATTY 6 cut(s) 202, 420, 743, 979, 1010, 1506
AcuI CTGAAG 1 cut(s) 1389
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 6 cut(s) 102, 168, 404, 410, 556, 1117
AfeI AGCGCT 1 cut(s) 1106
AfiI CCNNNNNNNGG 3 cut(s) 295, 310, 343
AjnI CCWGG 5 cut(s) 288, 336, 679, 822, 1353
AjuI GAANNNNNNNTTGG 4 cut(s) 686, 718, 1009, 1041
Alw21I GWGCWC 2 cut(s) 371, 672
Alw26I GTCTC 2 cut(s) 222, 1265
AlwI GGATC 5 cut(s) 195, 509, 881, 896, 1366
AlwNI CAGNNNCTG 1 cut(s) 190
Aor51HI AGCGCT 1 cut(s) 1106
AoxI GGCC 4 cut(s) 301, 346, 705, 1521
ApeKI GCWGC 4 cut(s) 469, 687, 1204, 1299
ApoI RAATTY 6 cut(s) 202, 420, 743, 979, 1010, 1506
Asp700I GAANNNNTTC 1 cut(s) 948
AspLEI GCGC 1 cut(s) 1107
AspS9I GGNCC 3 cut(s) 346, 916, 1522
AsuC2I CCSGG 1 cut(s) 1385
AsuII TTCGAA 1 cut(s) 162
AvaII GGWCC 1 cut(s) 916
BalI TGGCCA 2 cut(s) 303, 707
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 392
BbsI GAAGAC 1 cut(s) 1377
Bbv12I GWGCWC 2 cut(s) 371, 672
BbvI GCAGC 4 cut(s) 456, 674, 1216, 1311
BccI CCATC 3 cut(s) 378, 644, 1494
BceAI ACGGC 1 cut(s) 605
BciT130I CCWGG 5 cut(s) 290, 338, 681, 824, 1355
BciVI GTATCC 1 cut(s) 784
BclI TGATCA 1 cut(s) 264
BcnI CCSGG 1 cut(s) 1385
BcoDI GTCTC 2 cut(s) 222, 1265
BfaI CTAG 6 cut(s) 62, 96, 104, 108, 558, 1427
BfmI CTRYAG 1 cut(s) 1119
BfoI RGCGCY 1 cut(s) 1108
BfuI GTATCC 1 cut(s) 784
BglII AGATCT 1 cut(s) 1004
BisI GCNGC 4 cut(s) 470, 688, 1205, 1300
BlsI GCNGC 4 cut(s) 471, 689, 1206, 1301
BmcAI AGTACT 3 cut(s) 102, 168, 556
Bme1390I CCNGG 6 cut(s) 290, 338, 681, 824, 1355, 1385
Bme18I GGWCC 1 cut(s) 916
BmgT120I GGNCC 3 cut(s) 346, 916, 1522
BmiI GGNNCC 3 cut(s) 258, 389, 1524
BmrFI CCNGG 6 cut(s) 290, 338, 681, 824, 1355, 1385
BmsI GCATC 5 cut(s) 27, 138, 510, 598, 1297
BpiI GAAGAC 1 cut(s) 1377
BpmI CTGGAG 4 cut(s) 198, 410, 561, 702
Bpu14I TTCGAA 1 cut(s) 162
BpuEI CTTGAG 2 cut(s) 974, 1117
BpuMI CCSGG 1 cut(s) 1385
Bsa29I ATCGAT 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 587, 823, 1354
BsaWI WCCGGW 1 cut(s) 832
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 3 cut(s) 295, 310, 343
Bse1I ACTGG 3 cut(s) 321, 427, 609
Bse3DI GCAATG 2 cut(s) 8, 13
BseBI CCWGG 5 cut(s) 290, 338, 681, 824, 1355
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 3 cut(s) 587, 823, 1354
BseGI GGATG 2 cut(s) 613, 1057
BseLI CCNNNNNNNGG 3 cut(s) 295, 310, 343
BseMI GCAATG 2 cut(s) 8, 13
BseMII CTCAG 1 cut(s) 1260
BseNI ACTGG 3 cut(s) 321, 427, 609
BseRI GAGGAG 2 cut(s) 38, 212
BseXI GCAGC 4 cut(s) 456, 674, 1216, 1311
BseYI CCCAGC 1 cut(s) 1199
BshFI GGCC 4 cut(s) 303, 348, 707, 1523
BshNI GGYRCC 1 cut(s) 256
BshVI ATCGAT 1 cut(s) 504
BsiHKAI GWGCWC 2 cut(s) 371, 672
BsiSI CCGG 2 cut(s) 833, 1385
BslI CCNNNNNNNGG 3 cut(s) 295, 310, 343
BsmAI GTCTC 2 cut(s) 222, 1265
BsmBI CGTCTC 1 cut(s) 1265
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 4 cut(s) 303, 348, 707, 1523
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 3 cut(s) 371, 392, 672
BspACI CCGC 2 cut(s) 278, 690
BspANI GGCC 4 cut(s) 303, 348, 707, 1523
BspCNI CTCAG 1 cut(s) 1261
BspDI ATCGAT 1 cut(s) 504
BspHI TCATGA 1 cut(s) 1456
BspLI GGNNCC 3 cut(s) 258, 389, 1524
BspPI GGATC 5 cut(s) 195, 509, 881, 896, 1366
BspQI GCTCTTC 1 cut(s) 1114
BspT104I TTCGAA 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 256
BsrDI GCAATG 2 cut(s) 8, 13
BsrI ACTGG 3 cut(s) 321, 427, 609
BssECI CCNNGG 3 cut(s) 587, 823, 1354
BssNI GRCGYC 1 cut(s) 231
Bst2UI CCWGG 5 cut(s) 290, 338, 681, 824, 1355
Bst4CI ACNGT 3 cut(s) 171, 1180, 1333
Bst6I CTCTTC 5 cut(s) 69, 452, 723, 1114, 1222
BstACI GRCGYC 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 5 cut(s) 142, 1245, 1269, 1303, 1348
BstDSI CCRYGG 1 cut(s) 587
BstENI CCTNNNNNAGG 1 cut(s) 341
BstF5I GGATG 2 cut(s) 613, 1057
BstH2I RGCGCY 1 cut(s) 1108
BstHHI GCGC 1 cut(s) 1107
BstMAI GTCTC 2 cut(s) 222, 1265
BstMWI GCNNNNNNNGC 1 cut(s) 1296
BstNI CCWGG 5 cut(s) 290, 338, 681, 824, 1355
BstSCI CCNGG 6 cut(s) 288, 336, 679, 822, 1353, 1383
BstSFI CTRYAG 1 cut(s) 1119
BstV1I GCAGC 4 cut(s) 456, 674, 1216, 1311
BstV2I GAAGAC 1 cut(s) 1377
BstX2I RGATCY 4 cut(s) 187, 901, 1004, 1358
BstYI RGATCY 4 cut(s) 187, 901, 1004, 1358
Bsu15I ATCGAT 1 cut(s) 504
BsuI GTATCC 1 cut(s) 784
BsuRI GGCC 4 cut(s) 303, 348, 707, 1523
BsuTUI ATCGAT 1 cut(s) 504
BtgI CCRYGG 1 cut(s) 587
BtgZI GCGATG 1 cut(s) 660
BtsCI GGATG 2 cut(s) 613, 1057
BtsI GCAGTG 2 cut(s) 486, 672
BtsIMutI CAGTG 5 cut(s) 21, 260, 434, 486, 672
Cac8I GCNNGC 1 cut(s) 276
CaiI CAGNNNCTG 1 cut(s) 190
CciI TCATGA 1 cut(s) 1456
CfoI GCGC 1 cut(s) 1107
Cfr13I GGNCC 3 cut(s) 346, 916, 1522
ClaI ATCGAT 1 cut(s) 504
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 288
Csp6I GTAC 6 cut(s) 101, 167, 403, 409, 555, 1116
CviAII CATG 5 cut(s) 133, 496, 517, 1066, 1457
CviQI GTAC 6 cut(s) 101, 167, 403, 409, 555, 1116
DdeI CTNAG 5 cut(s) 142, 1245, 1269, 1303, 1348
EaeI YGGCCR 2 cut(s) 301, 705
Eam1104I CTCTTC 5 cut(s) 69, 452, 723, 1114, 1222
EarI CTCTTC 5 cut(s) 69, 452, 723, 1114, 1222
EciI GGCGGA 1 cut(s) 293
Eco24I GRGCYC 1 cut(s) 392
Eco32I GATATC 1 cut(s) 1129
Eco47I GGWCC 1 cut(s) 916
Eco47III AGCGCT 1 cut(s) 1106
Eco57I CTGAAG 1 cut(s) 1389
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoRII CCWGG 5 cut(s) 288, 336, 679, 822, 1353
EcoRV GATATC 1 cut(s) 1129
EcoT38I GRGCYC 1 cut(s) 392
Esp3I CGTCTC 1 cut(s) 1265
FaeI CATG 5 cut(s) 136, 499, 520, 1069, 1460
FatI CATG 5 cut(s) 132, 495, 516, 1065, 1456
FauI CCCGC 1 cut(s) 683
FauNDI CATATG 2 cut(s) 861, 1450
FbaI TGATCA 1 cut(s) 264
FblI GTMKAC 2 cut(s) 360, 1491
Fnu4HI GCNGC 4 cut(s) 470, 688, 1205, 1300
FokI GGATG 2 cut(s) 620, 1064
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 4 cut(s) 470, 688, 1205, 1300
FspBI CTAG 6 cut(s) 62, 96, 104, 108, 558, 1427
GlaI GCGC 1 cut(s) 1106
GluI GCNGC 4 cut(s) 470, 688, 1205, 1300
GsaI CCCAGC 1 cut(s) 1203
GsuI CTGGAG 4 cut(s) 198, 410, 561, 702
HaeII RGCGCY 1 cut(s) 1108
HaeIII GGCC 4 cut(s) 303, 348, 707, 1523
HapII CCGG 2 cut(s) 833, 1385
HgaI GACGC 1 cut(s) 239
HhaI GCGC 1 cut(s) 1107
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 5 cut(s) 136, 499, 520, 1069, 1460
Hin6I GCGC 1 cut(s) 1105
HinP1I GCGC 1 cut(s) 1105
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 7 cut(s) 243, 333, 656, 944, 1232, 1265, 1529
HpaII CCGG 2 cut(s) 833, 1385
Hpy166II GTNNAC 2 cut(s) 361, 1492
Hpy188I TCNGA 6 cut(s) 192, 444, 901, 1009, 1237, 1270
Hpy8I GTNNAC 2 cut(s) 361, 1492
Hpy99I CGWCG 3 cut(s) 233, 251, 362
HpyAV CCTTC 3 cut(s) 304, 403, 622
HpyCH4III ACNGT 3 cut(s) 171, 1180, 1333
HpyF10VI GCNNNNNNNGC 1 cut(s) 1296
HpyF3I CTNAG 5 cut(s) 142, 1245, 1269, 1303, 1348
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 5 cut(s) 136, 499, 520, 1069, 1460
HspAI GCGC 1 cut(s) 1105
Ksp22I TGATCA 1 cut(s) 264
LguI GCTCTTC 1 cut(s) 1114
LmnI GCTCC 3 cut(s) 387, 616, 682
Lsp1109I GCAGC 4 cut(s) 456, 674, 1216, 1311
LweI GCATC 5 cut(s) 27, 138, 510, 598, 1297
MabI ACCWGGT 1 cut(s) 288
MaeI CTAG 6 cut(s) 62, 96, 104, 108, 558, 1427
MaeIII GTNAC 2 cut(s) 994, 1343
MflI RGATCY 4 cut(s) 187, 901, 1004, 1358
MhlI GDGCHC 3 cut(s) 371, 392, 672
MlsI TGGCCA 2 cut(s) 303, 707
MluNI TGGCCA 2 cut(s) 303, 707
MlyI GAGTC 4 cut(s) 237, 342, 665, 1241
MmeI TCCRAC 2 cut(s) 580, 1215
MnlI CCTC 9 cut(s) 59, 233, 492, 677, 724, 915, 929, 1207, 1301
Mox20I TGGCCA 2 cut(s) 303, 707
MroXI GAANNNNTTC 1 cut(s) 948
MscI TGGCCA 2 cut(s) 303, 707
MseI TTAA 1 cut(s) 1040
Msp20I TGGCCA 2 cut(s) 303, 707
MspI CCGG 2 cut(s) 833, 1385
MspR9I CCNGG 6 cut(s) 290, 338, 681, 824, 1355, 1385
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 5 cut(s) 290, 338, 681, 824, 1355
MwoI GCNNNNNNNGC 1 cut(s) 1296
NciI CCSGG 1 cut(s) 1385
NdeI CATATG 2 cut(s) 861, 1450
NlaIII CATG 5 cut(s) 136, 499, 520, 1069, 1460
NlaIV GGNNCC 3 cut(s) 258, 389, 1524
NmuCI GTSAC 1 cut(s) 994
NspV TTCGAA 1 cut(s) 162
PagI TCATGA 1 cut(s) 1456
PciSI GCTCTTC 1 cut(s) 1114
PctI GAATGC 1 cut(s) 158
PdmI GAANNNNTTC 1 cut(s) 948
PfeI GAWTC 3 cut(s) 944, 1265, 1529
PflMI CCANNNNNTGG 1 cut(s) 295
PfoI TCCNGGA 1 cut(s) 679
PkrI GCNGC 4 cut(s) 471, 689, 1206, 1301
PleI GAGTC 4 cut(s) 237, 341, 664, 1240
PpsI GAGTC 4 cut(s) 237, 341, 664, 1240
PsiI TTATAA 1 cut(s) 285
Psp6I CCWGG 5 cut(s) 288, 336, 679, 822, 1353
PspFI CCCAGC 1 cut(s) 1199
PspGI CCWGG 5 cut(s) 288, 336, 679, 822, 1353
PspN4I GGNNCC 3 cut(s) 258, 389, 1524
PspPI GGNCC 3 cut(s) 346, 916, 1522
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 4 cut(s) 187, 901, 1004, 1358
RsaI GTAC 6 cut(s) 102, 168, 404, 410, 556, 1117
RsaNI GTAC 6 cut(s) 101, 167, 403, 409, 555, 1116
SalI GTCGAC 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 1114
SaqAI TTAA 1 cut(s) 1040
SatI GCNGC 4 cut(s) 470, 688, 1205, 1300
Sau96I GGNCC 3 cut(s) 346, 916, 1522
ScaI AGTACT 3 cut(s) 102, 168, 556
SchI GAGTC 4 cut(s) 237, 342, 665, 1241
ScrFI CCNGG 6 cut(s) 290, 338, 681, 824, 1355, 1385
SduI GDGCHC 3 cut(s) 371, 392, 672
SexAI ACCWGGT 1 cut(s) 288
SfaNI GCATC 5 cut(s) 27, 138, 510, 598, 1297
SfcI CTRYAG 1 cut(s) 1119
SfuI TTCGAA 1 cut(s) 162
SinI GGWCC 1 cut(s) 916
SmlI CTYRAG 2 cut(s) 953, 1132
SmoI CTYRAG 2 cut(s) 953, 1132
SsiI CCGC 2 cut(s) 278, 690
SspI AATATT 2 cut(s) 31, 757
SspMI CTAG 6 cut(s) 62, 96, 104, 108, 558, 1427
StyD4I CCNGG 6 cut(s) 288, 336, 679, 822, 1353, 1383
TaaI ACNGT 3 cut(s) 171, 1180, 1333
TatI WGTACW 4 cut(s) 100, 166, 402, 554
TfiI GAWTC 3 cut(s) 944, 1265, 1529
Tru1I TTAA 1 cut(s) 1040
Tru9I TTAA 1 cut(s) 1040
TscAI CASTG 5 cut(s) 28, 267, 434, 486, 679
TseFI GTSAC 1 cut(s) 994
TseI GCWGC 4 cut(s) 469, 687, 1204, 1299
Tsp45I GTSAC 1 cut(s) 994
TspDTI ATGAA 7 cut(s) 196, 632, 741, 1082, 1445, 1473, 1530
TspGWI ACGGA 1 cut(s) 1173
TspRI CASTG 5 cut(s) 28, 267, 434, 486, 679
Van91I CCANNNNNTGG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 916
XagI CCTNNNNNAGG 1 cut(s) 341
XapI RAATTY 6 cut(s) 202, 420, 743, 979, 1010, 1506
XbaI TCTAGA 1 cut(s) 1426
XcmI CCANNNNNNNNNTGG 1 cut(s) 296
XmiI GTMKAC 2 cut(s) 360, 1491
XmnI GAANNNNTTC 1 cut(s) 948
XspI CTAG 6 cut(s) 62, 96, 104, 108, 558, 1427
ZrmI AGTACT 3 cut(s) 102, 168, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.