RLG00000021971

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000039
Physical Location & Seq
Forward (+)
79636877 .. 79642386
5510 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021971

Sequence Viewer

Length: 2367 bp
ATGGCCGTGGTGTCGGACTTCGTTGTAGATAAAGCATTCGAAGCACTGTATGATGGTGTTAAAGACCTGATTGTCAAGAATTTCATTTTTAAGTCTCTCTTCAAGGACGTCAAATCCAGGCTCGACGATTGGGCAACACTAATCAAGAAGATGGCGCAATATAACCAGAAAATGGATCGCCCAGAGAAGGAACTGGAAGCTTTAAAGGAAGTTAGGGAGAAGGGCCAAGAGCTCGTCAACAAGTGCTTAACGATTACGCGATGGAGCCCCTTCAAAAAGTACAAGTACGCCACGCAACTTCTCCAATGGCAGGAATCTCTCAAAAGCCCGTTGGAGACGCTGAAAGTGCAAGAATCAGTGAATATAATGGAGATCAAGGTCGTGGTTGATGATATGCAACGTGTGATGCTGAAACAACAATCCGCAGAAAGTTCAAGTAGTACACGACTCGAACTTCCACCACTTCCACCGCTTATAGTTGGACTGGATGGTCCTTTGAATGATTTGAAGAAAAAGCTTCTTGCGGATGATGGGGTGTCGAGGCTTGTGCTCACAGCTCACTGCTCCTGGAGGCTGTGGGAAAACCACTCTCGCCACAATAAAGATATTTTCTTGTTTCAAAAAAAAAAGAGCTCGAATGCATTCGAAACTATTTGGTCAAAATCAGTACAACCCGGTAAATTCAAGAACAATGTTATCTTCATTTCTGTTTCACAAAAAGGCAATTTGGAACTTGTTGTGAAACGACTTTGTGAATCCGTGAGTTCTCCAGTTCCTACTTTCCAAAACAAAGTACATATGCTTCAATGGATGCAAACATTTTTCAAGGGAAGACCAAATCCTTTGCTGTTGGTCCTCGATGATATTTGGTCTGGATCATCAGAATCCATTCTTCATAGCTTTCAATTTGAATTATCCAATTACAAGATTTTAGTAACATCAAGATCTGAATTTCCAAATTTTGGTTCATCATATCACTTAAAATTATTGGATTATGACAAAGCTATGGAACTTTTTTATCACTCAACATCATTGGGAGATAAGCGCTCTACTGAACAAGAAGAACTCTCAAGAAAGATAGTCAAGCGTTGCAACGGATTTCCACTTGCTATTACAGTGGTTGGAAGATCACTTTCTGGGCAACCTATAGAGCTCTGGAGAAAAAGAGTATTTGAATGGTCTAAAGATTCTTCTATTCTAGATGCTGAGACGGAATTGTTTCTTTGTCTTCAAAGCAGCTTAGATGCTTTGGATAAAAGACTTGCAATTGTCAAAGATAGTTACTTGGACCTGGGATCTTTTCCTGAAGACAAAAGAATCCGAGTTGATGACCTCATTGATATGTGGGCGGAGTTATATGATATAGATGATGACAGTGTGTGCTTTGCAAATGTCTATGAACTTGCCAACAGGAATCTGGCTAATCTGGTTGACAATAGGAGGAGCCACTTGAAGATGGAGGGGGACGGTTACTACGGTGAACGGTATGTCACCCAACATGATTTGCTTAGAGAGTTAGCTATTTACAATGCTAAGCTAGACCCCGTAGAGCAAAGAAAAAGAACGAACATAGAAATAAGGGGAAACAAAATCCCCAGGTGGTGGAGAGAACAAAAATGTCACCCTATCAAGGCTCGCTTGTTATCTATTACTACAACTACTGACTTCGATATGGAATGGCCCAACTTGCAACTACCAGAAGTTTTTGTCTTGCATTTTCAGAGCGCAAGCCCAAGTAGTTACAGCTTGCCCGCGTTTGTACAGAAAATGGATAAATTGAAGCCCATACAACTGCAAAGTCTAAAGAAGATATCTTTCCAGACATGTCAAATGAGCGGTATCGGCCAAGGTGTTTTCAGAATGTCGGATGCATTTCCGAATTTGAAGGAATTAAACATTCAGTCCTGTTATGGGGTTGAAGAATTTCTCGTGGAGTGCTGCAATCTGATTCATTTAAAGAAGATTAGTATCGTCCGGAGTTTTATGCTATGGGACTTGCCCGAAGAAATCGGAAATTTGGTCAGCTTAGAAGTACTGAGGCTAAGGTCTTGTGATTTTCTTAACCTTCCCAGTTCCATCAGCAACCTCAAAAAGTTAAACTTTCTTGACATATCTGAATGCCGTCGCAGTGAGTTGCCTAAAAACATTGGTGAATTGAGCAGTTTAAGAAAGGTGGACATGAGGGATTGCTACGGAATGAACGAGCTGCCTCCATCAGTTTCGGATCTTCATCAGTTAGAGGAAGTGATATGCAACGATGAAGACCTAGGCAGTTTATGGGCAGCCTTTCTACCCACCCTCACAAACCTACGCGTGGTGATAGAAGCAACGCGCAAACTGGGGACTGAATCCTGCAAACGCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

789

Amino Acids

90.92

Weight (kDa)

7.99

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 133 2.2e-15 Arabidopsis broad-spectrum mildew resistance protein RPW8
LRR_14 PF23598 623 - 708 1.9e-12 Leucine-rich repeat region
LRR_14 PF23598 695 - 755 1.2e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 71, 489
AatII GACGTC 1 cut(s) 111
AccB7I CCANNNNNTGG 3 cut(s) 172, 962, 1602
AccBSI CCGCTC 1 cut(s) 1836
AccII CGCG 5 cut(s) 259, 1754, 2313, 2332, 2361
AccIII TCCGGA 1 cut(s) 1974
AciI CCGC 6 cut(s) 423, 470, 524, 1349, 1752, 1836
AclWI GGATC 4 cut(s) 183, 883, 1303, 2232
AcoI YGGCCR 2 cut(s) 3, 1843
AcsI RAATTY 7 cut(s) 79, 680, 950, 958, 1879, 1922, 2014
AcuI CTGAAG 1 cut(s) 1326
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 7 cut(s) 281, 287, 442, 669, 795, 1761, 2034
AfeI AGCGCT 1 cut(s) 1046
AfiI CCNNNNNNNGG 8 cut(s) 172, 187, 310, 962, 1602, 1630, 1911, 2314
AflIII ACRYGT 3 cut(s) 400, 1823, 2311
AjnI CCWGG 4 cut(s) 116, 566, 1290, 1595
AjuI GAANNNNNNNTTGG 2 cut(s) 949, 981
Alw21I GWGCWC 4 cut(s) 234, 552, 635, 1155
Alw26I GTCTC 3 cut(s) 99, 329, 1202
AlwI GGATC 4 cut(s) 183, 883, 1303, 2232
Aor13HI TCCGGA 1 cut(s) 1974
Aor51HI AGCGCT 1 cut(s) 1046
AoxI GGCC 4 cut(s) 3, 223, 1679, 1843
ApeKI GCWGC 4 cut(s) 1236, 1938, 2206, 2282
ApoI RAATTY 7 cut(s) 79, 680, 950, 958, 1879, 1922, 2014
Asp700I GAANNNNTTC 4 cut(s) 641, 888, 1218, 1923
AspA2I CCTAGG 1 cut(s) 2266
AspLEI GCGC 5 cut(s) 157, 1047, 1727, 2334, 2363
AspS9I GGNCC 5 cut(s) 223, 491, 853, 1288, 1680
AsuC2I CCSGG 1 cut(s) 675
AsuHPI GGTGA 5 cut(s) 1483, 1490, 1613, 2162, 2329
AsuII TTCGAA 2 cut(s) 39, 645
AvaII GGWCC 3 cut(s) 491, 853, 1288
AvrII CCTAGG 1 cut(s) 2266
BanII GRGCYC 4 cut(s) 234, 269, 635, 1155
BarI GAAGNNNNNNTAC 2 cut(s) 786, 818
BauI CACGAG 1 cut(s) 1928
BbsI GAAGAC 4 cut(s) 838, 1220, 1314, 2268
Bbv12I GWGCWC 4 cut(s) 234, 552, 635, 1155
BbvI GCAGC 4 cut(s) 1248, 1925, 2193, 2294
BccI CCATC 8 cut(s) 47, 145, 255, 482, 524, 1450, 2084, 2221
BceAI ACGGC 1 cut(s) 2106
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BciT130I CCWGG 4 cut(s) 118, 568, 1292, 1597
BcnI CCSGG 1 cut(s) 675
BcoDI GTCTC 3 cut(s) 99, 329, 1202
BfaI CTAG 3 cut(s) 1199, 1538, 2267
BfmI CTRYAG 1 cut(s) 1146
BfoI RGCGCY 1 cut(s) 1048
BglII AGATCT 1 cut(s) 944
BisI GCNGC 4 cut(s) 1237, 1939, 2207, 2283
BlnI CCTAGG 1 cut(s) 2266
BlpI GCTNAGC 1 cut(s) 1533
BlsI GCNGC 4 cut(s) 1238, 1940, 2208, 2284
BmcAI AGTACT 1 cut(s) 2034
Bme1390I CCNGG 5 cut(s) 118, 568, 675, 1292, 1597
Bme18I GGWCC 3 cut(s) 491, 853, 1288
BmgT120I GGNCC 5 cut(s) 223, 491, 853, 1288, 1680
BmiI GGNNCC 2 cut(s) 266, 1445
BmrFI CCNGG 5 cut(s) 118, 568, 675, 1292, 1597
BmrI ACTGGG 2 cut(s) 2064, 2348
BmsI GCATC 5 cut(s) 396, 801, 1192, 1234, 1858
BmuI ACTGGG 2 cut(s) 2064, 2348
BpiI GAAGAC 4 cut(s) 838, 1220, 1314, 2268
BpmI CTGGAG 3 cut(s) 589, 753, 1177
Bpu10I CCTNAGC 1 cut(s) 2042
Bpu1102I GCTNAGC 1 cut(s) 1533
Bpu14I TTCGAA 2 cut(s) 39, 645
BpuEI CTTGAG 1 cut(s) 1054
BpuMI CCSGG 1 cut(s) 675
BsaBI GATNNNNATC 2 cut(s) 1967, 2229
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 5 cut(s) 6, 1291, 1595, 1846, 2266
BsaWI WCCGGW 1 cut(s) 1974
Bsc4I CCNNNNNNNGG 8 cut(s) 172, 187, 310, 962, 1602, 1630, 1911, 2314
Bse1I ACTGG 5 cut(s) 198, 489, 770, 2070, 2343
Bse8I GATNNNNATC 2 cut(s) 1967, 2229
BseAI TCCGGA 1 cut(s) 1974
BseBI CCWGG 4 cut(s) 118, 568, 1292, 1597
BseDI CCNNGG 5 cut(s) 6, 1291, 1595, 1846, 2266
BseGI GGATG 4 cut(s) 493, 532, 816, 1873
BseJI GATNNNNATC 2 cut(s) 1967, 2229
BseLI CCNNNNNNNGG 8 cut(s) 172, 187, 310, 962, 1602, 1630, 1911, 2314
BseMII CTCAG 2 cut(s) 1197, 2027
BseNI ACTGG 5 cut(s) 198, 489, 770, 2070, 2343
BseRI GAGGAG 1 cut(s) 1456
BseXI GCAGC 4 cut(s) 1248, 1925, 2193, 2294
Bsh1236I CGCG 5 cut(s) 259, 1754, 2313, 2332, 2361
BshFI GGCC 4 cut(s) 5, 225, 1681, 1845
BsiHKAI GWGCWC 4 cut(s) 234, 552, 635, 1155
BsiSI CCGG 2 cut(s) 675, 1975
BslFI GGGAC 3 cut(s) 1478, 2006, 2356
BslI CCNNNNNNNGG 8 cut(s) 172, 187, 310, 962, 1602, 1630, 1911, 2314
BsmAI GTCTC 3 cut(s) 99, 329, 1202
BsmBI CGTCTC 2 cut(s) 329, 1202
BsmFI GGGAC 3 cut(s) 1478, 2006, 2356
BsmI GAATGC 4 cut(s) 35, 641, 643, 2123
BsnI GGCC 4 cut(s) 5, 225, 1681, 1845
Bsp119I TTCGAA 2 cut(s) 39, 645
Bsp1286I GDGCHC 5 cut(s) 234, 269, 552, 635, 1155
Bsp13I TCCGGA 1 cut(s) 1974
Bsp1407I TGTACA 1 cut(s) 1759
Bsp143I GATC 7 cut(s) 175, 372, 875, 944, 1127, 1295, 2224
Bsp1720I GCTNAGC 1 cut(s) 1533
BspACI CCGC 6 cut(s) 423, 470, 524, 1349, 1752, 1836
BspANI GGCC 4 cut(s) 5, 225, 1681, 1845
BspCNI CTCAG 2 cut(s) 1198, 2028
BspEI TCCGGA 1 cut(s) 1974
BspFNI CGCG 5 cut(s) 259, 1754, 2313, 2332, 2361
BspLI GGNNCC 2 cut(s) 266, 1445
BspPI GGATC 4 cut(s) 183, 883, 1303, 2232
BspT104I TTCGAA 2 cut(s) 39, 645
BsrBI CCGCTC 1 cut(s) 1836
BsrGI TGTACA 1 cut(s) 1759
BsrI ACTGG 5 cut(s) 198, 489, 770, 2070, 2343
BssECI CCNNGG 5 cut(s) 6, 1291, 1595, 1846, 2266
BssMI GATC 7 cut(s) 175, 372, 875, 944, 1127, 1295, 2224
BssNI GRCGYC 1 cut(s) 108
BssSI CACGAG 1 cut(s) 1928
BssT1I CCWWGG 2 cut(s) 1846, 2266
Bst2BI CACGAG 1 cut(s) 1928
Bst2UI CCWGG 4 cut(s) 118, 568, 1292, 1597
Bst4CI ACNGT 6 cut(s) 48, 1117, 1376, 1469, 1478, 1485
Bst6I CTCTTC 1 cut(s) 104
BstACI GRCGYC 1 cut(s) 108
BstAUI TGTACA 1 cut(s) 1759
BstBI TTCGAA 2 cut(s) 39, 645
BstC8I GCNNGC 4 cut(s) 1636, 1729, 1748, 1752
BstDEI CTNAG 7 cut(s) 1206, 1240, 1508, 1533, 2026, 2036, 2042
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 4 cut(s) 493, 532, 816, 1873
BstFNI CGCG 5 cut(s) 259, 1754, 2313, 2332, 2361
BstH2I RGCGCY 1 cut(s) 1048
BstHHI GCGC 5 cut(s) 157, 1047, 1727, 2334, 2363
BstKTI GATC 7 cut(s) 178, 375, 878, 947, 1130, 1298, 2227
BstMAI GTCTC 3 cut(s) 99, 329, 1202
BstMBI GATC 7 cut(s) 175, 372, 875, 944, 1127, 1295, 2224
BstMWI GCNNNNNNNGC 4 cut(s) 41, 346, 1687, 1842
BstNI CCWGG 4 cut(s) 118, 568, 1292, 1597
BstNSI RCATGY 1 cut(s) 1827
BstSCI CCNGG 5 cut(s) 116, 566, 673, 1290, 1595
BstSFI CTRYAG 1 cut(s) 1146
BstUI CGCG 5 cut(s) 259, 1754, 2313, 2332, 2361
BstV1I GCAGC 4 cut(s) 1248, 1925, 2193, 2294
BstV2I GAAGAC 4 cut(s) 838, 1220, 1314, 2268
BstX2I RGATCY 3 cut(s) 944, 1295, 2224
BstYI RGATCY 3 cut(s) 944, 1295, 2224
BsuRI GGCC 4 cut(s) 5, 225, 1681, 1845
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 4 cut(s) 493, 532, 816, 1873
BtsI GCAGTG 2 cut(s) 559, 2134
BtsIMutI CAGTG 6 cut(s) 44, 363, 559, 1122, 1381, 2134
Cac8I GCNNGC 4 cut(s) 1636, 1729, 1748, 1752
CfoI GCGC 5 cut(s) 157, 1047, 1727, 2334, 2363
Cfr13I GGNCC 5 cut(s) 223, 491, 853, 1288, 1680
CseI GACGC 1 cut(s) 346
Csp6I GTAC 7 cut(s) 280, 286, 441, 668, 794, 1760, 2033
CviAII CATG 3 cut(s) 1499, 1824, 2179
CviQI GTAC 7 cut(s) 280, 286, 441, 668, 794, 1760, 2033
DdeI CTNAG 7 cut(s) 1206, 1240, 1508, 1533, 2026, 2036, 2042
DpnI GATC 7 cut(s) 177, 374, 877, 946, 1129, 1297, 2226
DpnII GATC 7 cut(s) 175, 372, 875, 944, 1127, 1295, 2224
DraI TTTAAA 2 cut(s) 204, 1956
DrdI GACNNNNNNGTC 2 cut(s) 71, 489
DseDI GACNNNNNNGTC 2 cut(s) 71, 489
EaeI YGGCCR 2 cut(s) 3, 1843
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 1 cut(s) 1364
Ecl136II GAGCTC 3 cut(s) 232, 633, 1153
Eco130I CCWWGG 2 cut(s) 1846, 2266
Eco24I GRGCYC 4 cut(s) 234, 269, 635, 1155
Eco32I GATATC 1 cut(s) 1812
Eco47I GGWCC 3 cut(s) 491, 853, 1288
Eco47III AGCGCT 1 cut(s) 1046
Eco53kI GAGCTC 3 cut(s) 232, 633, 1153
Eco57I CTGAAG 1 cut(s) 1326
EcoICRI GAGCTC 3 cut(s) 232, 633, 1153
EcoRII CCWGG 4 cut(s) 116, 566, 1290, 1595
EcoRV GATATC 1 cut(s) 1812
EcoT14I CCWWGG 2 cut(s) 1846, 2266
EcoT22I ATGCAT 2 cut(s) 643, 1873
EcoT38I GRGCYC 4 cut(s) 234, 269, 635, 1155
ErhI CCWWGG 2 cut(s) 1846, 2266
Esp3I CGTCTC 2 cut(s) 329, 1202
FaeI CATG 3 cut(s) 1502, 1827, 2182
FalI AAGNNNNNCTT 6 cut(s) 83, 115, 1622, 1654, 2084, 2116
FaqI GGGAC 3 cut(s) 1478, 2006, 2356
FatI CATG 3 cut(s) 1498, 1823, 2178
FauI CCCGC 1 cut(s) 1759
FauNDI CATATG 1 cut(s) 798
Fnu4HI GCNGC 4 cut(s) 1237, 1939, 2207, 2283
FokI GGATG 4 cut(s) 500, 539, 823, 1880
FriOI GRGCYC 4 cut(s) 234, 269, 635, 1155
Fsp4HI GCNGC 4 cut(s) 1237, 1939, 2207, 2283
FspBI CTAG 3 cut(s) 1199, 1538, 2267
GlaI GCGC 5 cut(s) 156, 1046, 1726, 2333, 2362
GluI GCNGC 4 cut(s) 1237, 1939, 2207, 2283
GsuI CTGGAG 3 cut(s) 589, 753, 1177
HaeII RGCGCY 1 cut(s) 1048
HaeIII GGCC 4 cut(s) 5, 225, 1681, 1845
HapII CCGG 2 cut(s) 675, 1975
HgaI GACGC 1 cut(s) 346
HhaI GCGC 5 cut(s) 157, 1047, 1727, 2334, 2363
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 3 cut(s) 1502, 1827, 2182
Hin6I GCGC 5 cut(s) 155, 1045, 1725, 2332, 2361
HinP1I GCGC 5 cut(s) 155, 1045, 1725, 2332, 2361
HincII GTYRAC 2 cut(s) 238, 1432
HindII GTYRAC 2 cut(s) 238, 1432
HindIII AAGCTT 2 cut(s) 198, 515
HpaII CCGG 2 cut(s) 675, 1975
HphI GGTGA 5 cut(s) 1483, 1490, 1613, 2162, 2329
Hpy166II GTNNAC 5 cut(s) 238, 443, 1432, 1481, 2176
Hpy8I GTNNAC 5 cut(s) 238, 443, 1432, 1481, 2176
Hpy99I CGWCG 2 cut(s) 128, 2127
HpyAV CCTTC 5 cut(s) 181, 214, 280, 1879, 2075
HpyCH4III ACNGT 6 cut(s) 48, 1117, 1376, 1469, 1478, 1485
HpyCH4IV ACGT 2 cut(s) 108, 400
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 346, 1687, 1842
HpyF3I CTNAG 7 cut(s) 1206, 1240, 1508, 1533, 2026, 2036, 2042
HpySE526I ACGT 2 cut(s) 108, 400
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 3 cut(s) 1502, 1827, 2182
HspAI GCGC 5 cut(s) 155, 1045, 1725, 2332, 2361
Kpn2I TCCGGA 1 cut(s) 1974
Kzo9I GATC 7 cut(s) 175, 372, 875, 944, 1127, 1295, 2224
LmnI GCTCC 3 cut(s) 264, 569, 1443
Lsp1109I GCAGC 4 cut(s) 1248, 1925, 2193, 2294
LweI GCATC 5 cut(s) 396, 801, 1192, 1234, 1858
MaeI CTAG 3 cut(s) 1199, 1538, 2267
MaeII ACGT 2 cut(s) 108, 400
MaeIII GTNAC 6 cut(s) 934, 1280, 1469, 1489, 1619, 1739
MalI GATC 7 cut(s) 177, 374, 877, 946, 1129, 1297, 2226
MbiI CCGCTC 1 cut(s) 1836
MboI GATC 7 cut(s) 175, 372, 875, 944, 1127, 1295, 2224
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 3 cut(s) 944, 1295, 2224
MhlI GDGCHC 5 cut(s) 234, 269, 552, 635, 1155
MluI ACGCGT 1 cut(s) 2311
MlyI GAGTC 1 cut(s) 441
MmeI TCCRAC 4 cut(s) 312, 460, 1102, 1845
Mph1103I ATGCAT 2 cut(s) 643, 1873
MroI TCCGGA 1 cut(s) 1974
MroXI GAANNNNTTC 4 cut(s) 641, 888, 1218, 1923
MslI CAYNNNNRTG 1 cut(s) 1340
MspI CCGG 2 cut(s) 675, 1975
MspR9I CCNGG 5 cut(s) 118, 568, 675, 1292, 1597
MunI CAATTG 1 cut(s) 1266
Mva1269I GAATGC 4 cut(s) 35, 641, 643, 2123
MvaI CCWGG 4 cut(s) 118, 568, 1292, 1597
MvnI CGCG 5 cut(s) 259, 1754, 2313, 2332, 2361
MwoI GCNNNNNNNGC 4 cut(s) 41, 346, 1687, 1842
NciI CCSGG 1 cut(s) 675
NdeI CATATG 1 cut(s) 798
NdeII GATC 7 cut(s) 175, 372, 875, 944, 1127, 1295, 2224
NlaIII CATG 3 cut(s) 1502, 1827, 2182
NlaIV GGNNCC 2 cut(s) 266, 1445
NmuCI GTSAC 2 cut(s) 1489, 1619
NsiI ATGCAT 2 cut(s) 643, 1873
NspI RCATGY 1 cut(s) 1827
NspV TTCGAA 2 cut(s) 39, 645
PciI ACATGT 1 cut(s) 1823
PcsI WCGNNNNNNNCGW 1 cut(s) 2199
PctI GAATGC 4 cut(s) 35, 641, 643, 2123
PdmI GAANNNNTTC 4 cut(s) 641, 888, 1218, 1923
PfeI GAWTC 9 cut(s) 314, 353, 755, 884, 1187, 1317, 1414, 1948, 2348
PflMI CCANNNNNTGG 3 cut(s) 172, 962, 1602
PfoI TCCNGGA 1 cut(s) 566
PkrI GCNGC 4 cut(s) 1238, 1940, 2208, 2284
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
PscI ACATGT 1 cut(s) 1823
Psp124BI GAGCTC 3 cut(s) 234, 635, 1155
Psp6I CCWGG 4 cut(s) 116, 566, 1290, 1595
PspGI CCWGG 4 cut(s) 116, 566, 1290, 1595
PspN4I GGNNCC 2 cut(s) 266, 1445
PspPI GGNCC 5 cut(s) 223, 491, 853, 1288, 1680
PsuI RGATCY 3 cut(s) 944, 1295, 2224
RsaI GTAC 7 cut(s) 281, 287, 442, 669, 795, 1761, 2034
RsaNI GTAC 7 cut(s) 280, 286, 441, 668, 794, 1760, 2033
RseI CAYNNNNRTG 1 cut(s) 1340
SacI GAGCTC 3 cut(s) 234, 635, 1155
SatI GCNGC 4 cut(s) 1237, 1939, 2207, 2283
Sau3AI GATC 7 cut(s) 175, 372, 875, 944, 1127, 1295, 2224
Sau96I GGNCC 5 cut(s) 223, 491, 853, 1288, 1680
ScaI AGTACT 1 cut(s) 2034
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 5 cut(s) 118, 568, 675, 1292, 1597
SduI GDGCHC 5 cut(s) 234, 269, 552, 635, 1155
SfaNI GCATC 5 cut(s) 396, 801, 1192, 1234, 1858
SfcI CTRYAG 1 cut(s) 1146
SfuI TTCGAA 2 cut(s) 39, 645
SinI GGWCC 3 cut(s) 491, 853, 1288
SmiMI CAYNNNNRTG 1 cut(s) 1340
SmlI CTYRAG 1 cut(s) 1069
SmoI CTYRAG 1 cut(s) 1069
SsiI CCGC 6 cut(s) 423, 470, 524, 1349, 1752, 1836
SspMI CTAG 3 cut(s) 1199, 1538, 2267
SstI GAGCTC 3 cut(s) 234, 635, 1155
StyD4I CCNGG 5 cut(s) 116, 566, 673, 1290, 1595
StyI CCWWGG 2 cut(s) 1846, 2266
TaaI ACNGT 6 cut(s) 48, 1117, 1376, 1469, 1478, 1485
TaiI ACGT 2 cut(s) 111, 403
TaqI TCGA 8 cut(s) 39, 123, 450, 539, 635, 645, 858, 1668
TatI WGTACW 6 cut(s) 279, 440, 667, 793, 1759, 2032
TfiI GAWTC 9 cut(s) 314, 353, 755, 884, 1187, 1317, 1414, 1948, 2348
TscAI CASTG 6 cut(s) 51, 363, 566, 1122, 1381, 2134
TseFI GTSAC 2 cut(s) 1489, 1619
TseI GCWGC 4 cut(s) 1236, 1938, 2206, 2282
Tsp45I GTSAC 2 cut(s) 1489, 1619
TspDTI ATGAA 9 cut(s) 73, 691, 884, 957, 1413, 1940, 2213, 2219, 2274
TspGWI ACGGA 4 cut(s) 748, 1110, 1226, 2208
TspRI CASTG 6 cut(s) 51, 363, 566, 1122, 1381, 2134
Van91I CCANNNNNTGG 3 cut(s) 172, 962, 1602
VpaK11BI GGWCC 3 cut(s) 491, 853, 1288
XapI RAATTY 7 cut(s) 79, 680, 950, 958, 1879, 1922, 2014
XbaI TCTAGA 1 cut(s) 1198
XceI RCATGY 1 cut(s) 1827
XcmI CCANNNNNNNNNTGG 1 cut(s) 1414
XmaJI CCTAGG 1 cut(s) 2266
XmnI GAANNNNTTC 4 cut(s) 641, 888, 1218, 1923
XspI CTAG 3 cut(s) 1199, 1538, 2267
ZraI GACGTC 1 cut(s) 109
ZrmI AGTACT 1 cut(s) 2034
Zsp2I ATGCAT 2 cut(s) 643, 1873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.