Rh3BG269100

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000039
Physical Location & Seq
Reverse (-)
26150400 .. 26238761
88362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG269100.1

Sequence Viewer

Length: 3879 bp
ATGGCGGACAGAAAGAAATCTAAGAAACCCACAAATAACAACATCGAGCACCTCAAGACTGACATAGCCTCCTTCGCTTCCTCCCTCGGCCTCTCTACCTCTCTCCCTCCCTCTGATTCTTCAGGCTTCAACGATGTCGATTTCCGCAACCCCAAAAAACCCCAACAGAGGCCTAAACCCTCAAAACCCCAGAACCGGAACACCCAGAATTCCAAACCCCAAAATCGAAACACCACCCAGAGCTTCAAACCCAATGAAAGGCCCAATCTTTCGAAACCCCCTTTTCCTTCACTCCCTGATGTCACCAGCAACAGTGAAAAAGCAAAGACCTTTGAGAATCTTCCGAAGCTGCCGTTGATTTCAGCCACCAACATTGGAGTTTGGTACGAAGAAGCTGAAGAATTGGAAGGCAAAGTGGTCGGGAAGATGAAGAAAGCCGAGGCAAGGAGTGATAAGGAGTGGAGTGGTGAGGTGGCCAAGAAGCGAAAGCTTGGAGAGAGGTTGATGGCACAGTACACGGCGGATTACGAGGCTTCGAAAGGGAAGAGTGGGGATATCAAGCTGTTGGTGACCACACAGAGGTCAGGGACTGCTTCTGATAAGATTTCGGCGTTTTCGGTGTTGGTTGGGGATGATCCCATTGCCAATTTGAGGTCTTTGGATGCACTTTTAGGTTCGGCTTGGGATTGGACTGGTACTGATACTGATACAGAAGAAGGGCTGGGTTCGGGAAGCAAATCGGTTCCGTCGGCGATGATGACGTCATCAGCGTCGAAAGAGAGAGATGCAAGAAACAATCCTTATATTCACGTCTACTTTGATCTCGCATTATCGATCTCAGCAATGGTGATAATCTGCGGAGCTGGTTTAGCGGTGACATTTTGGGCGCTGTATGATTCTGTTAAGCAAGTGAAGGACCAGATACTGATGTTCAAACCCCAACTCGGGGATATCAAGTCCACTCTGGACTCTTTGCAGCCACTGATCAAGGAGATGGCACAGTGTGAGCAGGAAATGGACGGTCCAGAGGAGGAACTAGAGAATCTTAGAGTGGAAATGGAGGAAGGAGTGGAGCTTATTCGCAAGTGTTCGGAAGCGAGCAGATTCAAGAAGTATAAGTATGGCAGCAGACTTGTTGAATTGAGCAAGTCACTTCAGAGGCTGTTGGAGATATTGAAAGTGCAGGGCATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGGAACATTGAAAATGTGGTGCACAGAATTGAAGGGAATTGTGTGATACAGAACAATCAGTCCGAAAAAATTGAAGGGTGGTGTGCTGTGCCTGAGCCTCCAACAATTACGGTTGGATTGGATTTGCCTTTGAGGGAATTGAAGATGAAGCTCTTTACTGACGGAGTTTCGATGGTGATAATTACTGCTCCAGGAGGATGTGGAAAAACTACACTGGCAACAAAGTTGTGCCAAGATGCAGAGGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACTTATACCTAATTGTAAAAGAGCTATATCATCGAACGGGTGCCCAGGTCCCCGATTTCCAAAGTGAAGTAACTGCAGTTAGCTGGCTGCAGCAATTTCTGAAGCAAACAGGACAAAATCCTATGTTGTTGATCCTGGATGATGTTTGGGCTGGATCAGAATTCATTCTTGAGAAGTTCGATGAATTAAAATTGACAAATTACAAGATCTTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTATCTAGAATGTTTGAACCACAAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGAGACAGGAGCTCTTACATTTCAGAAGATCTTCCAACAAAGATAGTAGAACGTTGTATGGGATTTCCACTTGCAATTACAGTTGTTGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTAATGGAATGGTCTAGAGGTTCTTCTGTTCTTGACTCTGAACATGATTTGCTTGATTGCCTCCAAAGTAGTTTAGATGTTTTGTATGCAGAGAAGCCTCTTATGAGGGAATGCTTTATAGACCTTGGTTTATTTCCTGAAGATCAAAGAATCCCTACCACTGTCCTGATTGATATGTGGGCAGAGTTATATGGCATACATGAAGACATTTCGTCCATTGCGAACCTCTATGAACTTGCCTCTCGAAGTTTGGCTAACCTGGTAGTCACAAGGAAGGACAACAAGGATAGTGATGGCTATTACACTGAACACTTCGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCAGCAGTTGAGTCAGGAACCACTACAACATAGAATGAGGCTTATGATTGACATATGTGGAGACAACCTTCCGATGTGGTGGAAAGAACAAAAGTATCAATCCCTCATTGCTCGCCTTGTATCGATCTCAACAGACGAAGAGTTCTCATCTAAATGGCAAAACTTGCAACTACCAGAAGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTGGAGAAAATGAATAAACTGAAGACTCTAGCACTCACAAGTTCGGGTATCAGTCCTGCTGAGTTGAATAACTTTCAATTAGTTGATTCGAAACCAAATTTAAAGAGAATCAGATTAGAACGAATTTCATTTCCTTCGATAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTAGCATCGGCCAAGGTTTTGGTAACGGTTACATACATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGCTGCCTGCCGATCTCTGTGACCTTATTCAATTGAAGAAACTCAGTGTCACCAATTCTCATAAGATCTCTGTCTTACCCGAAGACATTGGAAATCTAGTAAATTTAGAGCTGCTGAGGCTTAGGTGTTGTACAGACTTGTCAGAGTTACCCGGCTCGATTAGGAACCTCAAGAACTTAAACTTGCTTGACATATCAAATTGCTTTAGCCTTAAGGAATTGCCTGAAGACATTGGAGAAATGTGCAGTTTAGAGAAGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCATCAGTTGTGGATCTTGAAAAGTTAATGGAAGTGACATGTGATGAAGAGACAGAAATCTTATGGGAGCCCTTCTTACCGTATCTCAGGAACATACACATAAAGGTTGTGAAAGAAGATATGAACCTGAATTGGCTCCACTATGACAGAATCAAGATGGTTGTGGTTATTGCTGCATCTGCTGTAGGACTAGCATTATCAGTAGTGCTGTATAAGGGCATTGAGGAAGTTATCAGAAAGTGCGTGATCGACAAGATTATGGAGTTTAATCCCCTCCTCGAGAAAATCAATATGAAGCTAAAGGAATTGGAGCCAGAGATAGAAGAGATCGAGAAAAGGAATCAAGAATTGGATCGCCCAGAGGAATTGAAAGATTTCACAAAAGTGATCGAGGATGGCATAGCGCTCGTTGATAAGTTAAAGGAGGTTGCCCATTGGTACAAGAAACCTAAGTACACCAACAAACTCAAACAACTTGATGAATCTCTGGATACACAGATAATGTTACTGCTGGTGCGGTCAGAAAGTAAGGTCGCTAACCTTTCAGTTATAGCAAGTATTACCGAGGGGAACACCACAAATATCCGTATGCAAATGGGGAATATGAGTATGCTAATGGGGAGTATGAGTATGCAAATAGAAAATATGGGTATGCAAATAGGGGATTTGAATACGAATCATGATACTATTTGTAGAGATGATCAGGGATATGCGACAGAATGGCCTAGTGCAAAATCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

1292

Amino Acids

146.17

Weight (kDa)

5.62

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 284 - 409 3.4e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 451 - 606 6.2e-15 NB-ARC domain
AAA_22 PF13401 464 - 565 9.3e-07 AAA domain
LRR_14 PF23598 931 - 1014 6.1e-09 Leucine-rich repeat region
LRR_14 PF23598 1016 - 1065 4.7e-07 Leucine-rich repeat region
RPW8 PF05659 1108 - 1210 7.5e-14 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1741
AatII GACGTC 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 2682
AccB1I GGYRCC 1 cut(s) 1559
AccB7I CCANNNNNTGG 1 cut(s) 1760
AccI GTMKAC 1 cut(s) 813
AciI CCGC 7 cut(s) 5, 145, 521, 858, 872, 1935, 3652
AclI AACGTT 1 cut(s) 1885
AclWI GGATC 5 cut(s) 629, 1645, 1681, 3156, 3495
AcoI YGGCCR 2 cut(s) 474, 2749
AcsI RAATTY 6 cut(s) 208, 1475, 1679, 2644, 2670, 2941
AcuI CTGAAG 7 cut(s) 105, 417, 1139, 1640, 2124, 2588, 3084
AcyI GRCGYC 1 cut(s) 761
AdeI CACNNNGTG 1 cut(s) 1004
AfaI GTAC 6 cut(s) 386, 515, 697, 2971, 3575, 3590
AfeI AGCGCT 1 cut(s) 3540
AflII CTTAAG 1 cut(s) 3050
AflIII ACRYGT 1 cut(s) 3173
AhdI GACNNNNNGTC 1 cut(s) 2176
AjiI CACGTC 1 cut(s) 811
AjnI CCWGG 4 cut(s) 1411, 1563, 1653, 2223
AjuI GAANNNNNNNTTGG 6 cut(s) 1193, 1225, 1747, 1779, 3467, 3499
AleI CACNNNNGTG 1 cut(s) 3518
AloI GAACNNNNNNTCC 2 cut(s) 1265, 1297
Alw21I GWGCWC 4 cut(s) 51, 1245, 1847, 3116
Alw26I GTCTC 4 cut(s) 1830, 2370, 3100, 3179
Alw44I GTGCAC 1 cut(s) 1241
AlwI GGATC 5 cut(s) 629, 1645, 1681, 3156, 3495
AlwNI CAGNNNCTG 4 cut(s) 590, 925, 982, 1162
Ama87I CYCGRG 3 cut(s) 944, 3115, 3413
Aor51HI AGCGCT 1 cut(s) 3540
AoxI GGCC 6 cut(s) 88, 170, 260, 474, 2749, 3857
ApaLI GTGCAC 1 cut(s) 1241
ApoI RAATTY 6 cut(s) 208, 1475, 1679, 2644, 2670, 2941
Asp700I GAANNNNTTC 3 cut(s) 1683, 1863, 3509
AspLEI GCGC 2 cut(s) 889, 3541
AspS9I GGNCC 4 cut(s) 261, 916, 1022, 1567
AsuC2I CCSGG 1 cut(s) 2991
AsuII TTCGAA 3 cut(s) 272, 536, 2636
AvaI CYCGRG 3 cut(s) 944, 3115, 3413
AvaII GGWCC 3 cut(s) 916, 1022, 1567
AxyI CCTNAGG 1 cut(s) 1831
BaeGI GKGCMC 2 cut(s) 1245, 1564
BaeI ACNNNNGTAYC 4 cut(s) 914, 947, 2393, 2426
BalI TGGCCA 1 cut(s) 476
BanI GGYRCC 1 cut(s) 1559
BanII GRGCYC 2 cut(s) 1847, 3207
BbsI GAAGAC 5 cut(s) 1483, 2175, 2576, 2928, 3072
Bbv12I GWGCWC 4 cut(s) 51, 1245, 1847, 3116
BbvCI CCTCAGC 1 cut(s) 2954
BccI CCATC 6 cut(s) 499, 988, 1387, 2252, 3286, 3524
BceAI ACGGC 2 cut(s) 337, 534
BcgI CGANNNNNNTGC 6 cut(s) 400, 434, 1197, 1231, 3523, 3557
BciT130I CCWGG 4 cut(s) 1413, 1565, 1655, 2225
BciVI GTATCC 2 cut(s) 2706, 3619
BclI TGATCA 2 cut(s) 984, 3835
BcnI CCSGG 1 cut(s) 2991
BcoDI GTCTC 4 cut(s) 1830, 2370, 3100, 3179
BfaI CTAG 7 cut(s) 1037, 1778, 1980, 2576, 2936, 3326, 3861
BfmI CTRYAG 3 cut(s) 1593, 1607, 3318
BfoI RGCGCY 2 cut(s) 890, 3542
BfrI CTTAAG 1 cut(s) 3050
BfuAI ACCTGC 1 cut(s) 2682
BfuI GTATCC 2 cut(s) 2706, 3619
BglII AGATCT 3 cut(s) 1725, 1861, 2904
Bme1390I CCNGG 5 cut(s) 1413, 1565, 1655, 2225, 2991
Bme18I GGWCC 3 cut(s) 916, 1022, 1567
BmeRI GACNNNNNGTC 1 cut(s) 2176
BmeT110I CYCGRG 3 cut(s) 944, 3115, 3413
BmgBI CACGTC 1 cut(s) 811
BmgT120I GGNCC 4 cut(s) 261, 916, 1022, 1567
BmiI GGNNCC 8 cut(s) 744, 1561, 1569, 2334, 3005, 3204, 3272, 3447
BmrFI CCNGG 5 cut(s) 1413, 1565, 1655, 2225, 2991
BmsI GCATC 7 cut(s) 652, 775, 1447, 1789, 1835, 2754, 3320
BpiI GAAGAC 5 cut(s) 1483, 2175, 2576, 2928, 3072
BpmI CTGGAG 1 cut(s) 1395
Bpu10I CCTNAGC 4 cut(s) 1314, 2300, 2954, 2960
Bpu14I TTCGAA 3 cut(s) 272, 536, 2636
BpuEI CTTGAG 3 cut(s) 38, 1709, 2993
BpuMI CCSGG 1 cut(s) 2991
Bsa29I ATCGAT 2 cut(s) 833, 2438
BsaHI GRCGYC 1 cut(s) 761
BsaJI CCNNGG 6 cut(s) 85, 438, 1563, 2089, 2752, 3699
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 3748, 3778
Bse1I ACTGG 2 cut(s) 697, 1440
Bse21I CCTNAGG 1 cut(s) 1831
Bse3DI GCAATG 7 cut(s) 639, 849, 1492, 1809, 2181, 2421, 2833
BseBI CCWGG 4 cut(s) 1413, 1565, 1655, 2225
BseCI ATCGAT 2 cut(s) 833, 2438
BseDI CCNNGG 6 cut(s) 85, 438, 1563, 2089, 2752, 3699
BseGI GGATG 7 cut(s) 637, 667, 1201, 1424, 1663, 1826, 3535
BseMI GCAATG 7 cut(s) 639, 849, 1492, 1809, 2181, 2421, 2833
BseMII CTCAG 7 cut(s) 852, 1305, 2532, 2598, 2896, 2945, 3235
BseNI ACTGG 2 cut(s) 697, 1440
BseRI GAGGAG 2 cut(s) 1043, 3401
BseSI GKGCMC 2 cut(s) 1245, 1564
BseYI CCCAGC 1 cut(s) 721
BsgI GTGCAG 2 cut(s) 1202, 3103
BshFI GGCC 6 cut(s) 90, 172, 262, 476, 2751, 3859
BshNI GGYRCC 1 cut(s) 1559
BshVI ATCGAT 2 cut(s) 833, 2438
BsiHKAI GWGCWC 4 cut(s) 51, 1245, 1847, 3116
BsiHKCI CYCGRG 3 cut(s) 944, 3115, 3413
BsiSI CCGG 2 cut(s) 196, 2991
BslFI GGGAC 2 cut(s) 601, 1553
BsmAI GTCTC 4 cut(s) 1830, 2370, 3100, 3179
BsmFI GGGAC 2 cut(s) 601, 1553
BsmI GAATGC 1 cut(s) 2081
BsnI GGCC 6 cut(s) 90, 172, 262, 476, 2751, 3859
BsoBI CYCGRG 3 cut(s) 944, 3115, 3413
Bsp119I TTCGAA 3 cut(s) 272, 536, 2636
Bsp1286I GDGCHC 6 cut(s) 51, 1245, 1564, 1847, 3116, 3207
Bsp1407I TGTACA 1 cut(s) 2969
BspACI CCGC 7 cut(s) 5, 145, 521, 858, 872, 1935, 3652
BspANI GGCC 6 cut(s) 90, 172, 262, 476, 2751, 3859
BspCNI CTCAG 7 cut(s) 851, 1306, 2533, 2599, 2895, 2946, 3234
BspDI ATCGAT 2 cut(s) 833, 2438
BspHI TCATGA 1 cut(s) 3814
BspLI GGNNCC 8 cut(s) 744, 1561, 1569, 2334, 3005, 3204, 3272, 3447
BspMAI CTGCAG 2 cut(s) 1597, 1611
BspMI ACCTGC 1 cut(s) 2682
BspPI GGATC 5 cut(s) 629, 1645, 1681, 3156, 3495
BspT104I TTCGAA 3 cut(s) 272, 536, 2636
BspT107I GGYRCC 1 cut(s) 1559
BspTI CTTAAG 1 cut(s) 3050
BsrDI GCAATG 7 cut(s) 639, 849, 1492, 1809, 2181, 2421, 2833
BsrGI TGTACA 1 cut(s) 2969
BsrI ACTGG 2 cut(s) 697, 1440
BssECI CCNNGG 6 cut(s) 85, 438, 1563, 2089, 2752, 3699
BssNI GRCGYC 1 cut(s) 761
BssT1I CCWWGG 2 cut(s) 2089, 2752
Bst2UI CCWGG 4 cut(s) 1413, 1565, 1655, 2225
Bst6I CTCTTC 4 cut(s) 539, 2448, 3177, 3453
BstACI GRCGYC 1 cut(s) 761
BstAFI CTTAAG 1 cut(s) 3050
BstAPI GCANNNNNTGC 2 cut(s) 2296, 2479
BstAUI TGTACA 1 cut(s) 2969
BstBI TTCGAA 3 cut(s) 272, 536, 2636
BstC8I GCNNGC 5 cut(s) 1099, 1604, 1937, 2428, 2847
BstEII GGTNACC 1 cut(s) 568
BstF5I GGATG 7 cut(s) 637, 667, 1201, 1424, 1663, 1826, 3535
BstH2I RGCGCY 2 cut(s) 890, 3542
BstHHI GCGC 2 cut(s) 889, 3541
BstMAI GTCTC 4 cut(s) 1830, 2370, 3100, 3179
BstMWI GCNNNNNNNGC 6 cut(s) 74, 869, 2296, 2479, 2956, 3314
BstNI CCWGG 4 cut(s) 1413, 1565, 1655, 2225
BstNSI RCATGY 2 cut(s) 2299, 3177
BstPI GGTNACC 1 cut(s) 568
BstSCI CCNGG 5 cut(s) 1411, 1563, 1653, 2223, 2989
BstSFI CTRYAG 3 cut(s) 1593, 1607, 3318
BstSLI GKGCMC 2 cut(s) 1245, 1564
BstV2I GAAGAC 5 cut(s) 1483, 2175, 2576, 2928, 3072
BstX2I RGATCY 4 cut(s) 1725, 1861, 2904, 3148
BstXI CCANNNNNNTGG 1 cut(s) 2759
BstYI RGATCY 4 cut(s) 1725, 1861, 2904, 3148
Bsu15I ATCGAT 2 cut(s) 833, 2438
Bsu36I CCTNAGG 1 cut(s) 1831
BsuI GTATCC 2 cut(s) 2706, 3619
BsuRI GGCC 6 cut(s) 90, 172, 262, 476, 2751, 3859
BsuTUI ATCGAT 2 cut(s) 833, 2438
BtgZI GCGATG 1 cut(s) 767
BtrI CACGTC 1 cut(s) 811
BtsCI GGATG 7 cut(s) 637, 667, 1201, 1424, 1663, 1826, 3535
BtsIMutI CAGTG 8 cut(s) 319, 980, 1007, 1433, 2124, 2268, 2890, 3116
BveI ACCTGC 1 cut(s) 2682
Cac8I GCNNGC 5 cut(s) 1099, 1604, 1937, 2428, 2847
CaiI CAGNNNCTG 4 cut(s) 590, 925, 982, 1162
CciI TCATGA 1 cut(s) 3814
CfoI GCGC 2 cut(s) 889, 3541
Cfr13I GGNCC 4 cut(s) 261, 916, 1022, 1567
ClaI ATCGAT 2 cut(s) 833, 2438
CseI GACGC 1 cut(s) 759
CsiI ACCWGGT 1 cut(s) 2223
Csp6I GTAC 6 cut(s) 385, 514, 696, 2970, 3574, 3589
CspCI CAANNNNNGTGG 2 cut(s) 355, 390
CviAII CATG 9 cut(s) 1820, 2009, 2165, 2291, 2296, 2737, 3103, 3174, 3815
CviQI GTAC 6 cut(s) 385, 514, 696, 2970, 3574, 3589
DraI TTTAAA 1 cut(s) 2649
DraIII CACNNNGTG 1 cut(s) 1004
DrdI GACNNNNNNGTC 1 cut(s) 1741
DriI GACNNNNNGTC 1 cut(s) 2176
DseDI GACNNNNNNGTC 1 cut(s) 1741
EaeI YGGCCR 2 cut(s) 474, 2749
Eam1104I CTCTTC 4 cut(s) 539, 2448, 3177, 3453
Eam1105I GACNNNNNGTC 1 cut(s) 2176
EarI CTCTTC 4 cut(s) 539, 2448, 3177, 3453
EciI GGCGGA 2 cut(s) 20, 536
Ecl136II GAGCTC 1 cut(s) 1845
Eco130I CCWWGG 2 cut(s) 2089, 2752
Eco147I AGGCCT 1 cut(s) 172
Eco24I GRGCYC 2 cut(s) 1847, 3207
Eco32I GATATC 2 cut(s) 556, 952
Eco47I GGWCC 3 cut(s) 916, 1022, 1567
Eco47III AGCGCT 1 cut(s) 3540
Eco53kI GAGCTC 1 cut(s) 1845
Eco57I CTGAAG 7 cut(s) 105, 417, 1139, 1640, 2124, 2588, 3084
Eco81I CCTNAGG 1 cut(s) 1831
Eco88I CYCGRG 3 cut(s) 944, 3115, 3413
Eco91I GGTNACC 1 cut(s) 568
EcoICRI GAGCTC 1 cut(s) 1845
EcoO109I RGGNCCY 1 cut(s) 1567
EcoO65I GGTNACC 1 cut(s) 568
EcoRI GAATTC 2 cut(s) 208, 1679
EcoRII CCWGG 4 cut(s) 1411, 1563, 1653, 2223
EcoRV GATATC 2 cut(s) 556, 952
EcoT14I CCWWGG 2 cut(s) 2089, 2752
EcoT38I GRGCYC 2 cut(s) 1847, 3207
ErhI CCWWGG 2 cut(s) 2089, 2752
FaeI CATG 9 cut(s) 1823, 2012, 2168, 2294, 2299, 2740, 3106, 3177, 3818
FalI AAGNNNNNCTT 4 cut(s) 1505, 1537, 1732, 1764
FaqI GGGAC 2 cut(s) 601, 1553
FatI CATG 9 cut(s) 1819, 2008, 2164, 2290, 2295, 2736, 3102, 3173, 3814
FauI CCCGC 1 cut(s) 1928
FauNDI CATATG 1 cut(s) 2369
FbaI TGATCA 2 cut(s) 984, 3835
FblI GTMKAC 1 cut(s) 813
FokI GGATG 7 cut(s) 644, 674, 1208, 1431, 1670, 1813, 3542
FriOI GRGCYC 2 cut(s) 1847, 3207
FspBI CTAG 7 cut(s) 1037, 1778, 1980, 2576, 2936, 3326, 3861
GlaI GCGC 2 cut(s) 888, 3540
GsaI CCCAGC 1 cut(s) 725
GsuI CTGGAG 1 cut(s) 1395
HaeII RGCGCY 2 cut(s) 890, 3542
HaeIII GGCC 6 cut(s) 90, 172, 262, 476, 2751, 3859
HapII CCGG 2 cut(s) 196, 2991
HgaI GACGC 1 cut(s) 759
HhaI GCGC 2 cut(s) 889, 3541
Hin1I GRCGYC 1 cut(s) 761
Hin1II CATG 9 cut(s) 1823, 2012, 2168, 2294, 2299, 2740, 3106, 3177, 3818
Hin6I GCGC 2 cut(s) 887, 3539
HinP1I GCGC 2 cut(s) 887, 3539
HindIII AAGCTT 1 cut(s) 488
HpaII CCGG 2 cut(s) 196, 2991
Hpy166II GTNNAC 5 cut(s) 516, 814, 960, 1243, 3591
Hpy8I GTNNAC 5 cut(s) 516, 814, 960, 1243, 3591
Hpy99I CGWCG 2 cut(s) 751, 775
HpyCH4IV ACGT 3 cut(s) 761, 810, 1885
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 869, 2296, 2479, 2956, 3314
HpySE526I ACGT 3 cut(s) 761, 810, 1885
Hsp92I GRCGYC 1 cut(s) 761
Hsp92II CATG 9 cut(s) 1823, 2012, 2168, 2294, 2299, 2740, 3106, 3177, 3818
HspAI GCGC 2 cut(s) 887, 3539
Ksp22I TGATCA 2 cut(s) 984, 3835
LmnI GCTCC 8 cut(s) 860, 1072, 1414, 1842, 2839, 3202, 3276, 3445
LweI GCATC 7 cut(s) 652, 775, 1447, 1789, 1835, 2754, 3320
MabI ACCWGGT 1 cut(s) 2223
MaeI CTAG 7 cut(s) 1037, 1778, 1980, 2576, 2936, 3326, 3861
MaeII ACGT 3 cut(s) 761, 810, 1885
MfeI CAATTG 1 cut(s) 2870
MflI RGATCY 4 cut(s) 1725, 1861, 2904, 3148
MhlI GDGCHC 6 cut(s) 51, 1245, 1564, 1847, 3116, 3207
MlsI TGGCCA 1 cut(s) 476
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 4 cut(s) 962, 1994, 2335, 2566
MmeI TCCRAC 5 cut(s) 1146, 1315, 1346, 1892, 1900
Mox20I TGGCCA 1 cut(s) 476
MroXI GAANNNNTTC 3 cut(s) 1683, 1863, 3509
MscI TGGCCA 1 cut(s) 476
MseI TTAA 9 cut(s) 903, 1706, 2648, 3017, 3051, 3161, 3402, 3554, 3877
MslI CAYNNNNRTG 3 cut(s) 1797, 2467, 3518
Msp20I TGGCCA 1 cut(s) 476
MspCI CTTAAG 1 cut(s) 3050
MspI CCGG 2 cut(s) 196, 2991
MspR9I CCNGG 5 cut(s) 1413, 1565, 1655, 2225, 2991
MunI CAATTG 1 cut(s) 2870
Mva1269I GAATGC 1 cut(s) 2081
MvaI CCWGG 4 cut(s) 1413, 1565, 1655, 2225
MwoI GCNNNNNNNGC 6 cut(s) 74, 869, 2296, 2479, 2956, 3314
NciI CCSGG 1 cut(s) 2991
NdeI CATATG 1 cut(s) 2369
NlaIII CATG 9 cut(s) 1823, 2012, 2168, 2294, 2299, 2740, 3106, 3177, 3818
NlaIV GGNNCC 8 cut(s) 744, 1561, 1569, 2334, 3005, 3204, 3272, 3447
NmeAIII GCCGAG 2 cut(s) 66, 463
NmuCI GTSAC 9 cut(s) 301, 568, 874, 1149, 1732, 2230, 2858, 2887, 3169
NspI RCATGY 2 cut(s) 2299, 3177
NspV TTCGAA 3 cut(s) 272, 536, 2636
OliI CACNNNNGTG 1 cut(s) 3518
PaeR7I CTCGAG 2 cut(s) 3115, 3413
PagI TCATGA 1 cut(s) 3814
PceI AGGCCT 1 cut(s) 172
PciI ACATGT 1 cut(s) 3173
PcsI WCGNNNNNNNCGW 2 cut(s) 767, 2183
PctI GAATGC 1 cut(s) 2081
PdmI GAANNNNTTC 3 cut(s) 1683, 1863, 3509
PflMI CCANNNNNTGG 1 cut(s) 1760
PfoI TCCNGGA 2 cut(s) 1411, 1653
PleI GAGTC 4 cut(s) 962, 1994, 2334, 2566
PpsI GAGTC 4 cut(s) 962, 1994, 2334, 2566
PpuMI RGGWCCY 1 cut(s) 1567
PscI ACATGT 1 cut(s) 3173
Psp124BI GAGCTC 1 cut(s) 1847
Psp1406I AACGTT 1 cut(s) 1885
Psp5II RGGWCCY 1 cut(s) 1567
Psp6I CCWGG 4 cut(s) 1411, 1563, 1653, 2223
PspEI GGTNACC 1 cut(s) 568
PspFI CCCAGC 1 cut(s) 721
PspGI CCWGG 4 cut(s) 1411, 1563, 1653, 2223
PspN4I GGNNCC 8 cut(s) 744, 1561, 1569, 2334, 3005, 3204, 3272, 3447
PspPI GGNCC 4 cut(s) 261, 916, 1022, 1567
PspPPI RGGWCCY 1 cut(s) 1567
PstI CTGCAG 2 cut(s) 1597, 1611
PstNI CAGNNNCTG 4 cut(s) 590, 925, 982, 1162
PsuI RGATCY 4 cut(s) 1725, 1861, 2904, 3148
RsaI GTAC 6 cut(s) 386, 515, 697, 2971, 3575, 3590
RsaNI GTAC 6 cut(s) 385, 514, 696, 2970, 3574, 3589
RseI CAYNNNNRTG 3 cut(s) 1797, 2467, 3518
SacI GAGCTC 1 cut(s) 1847
SaqAI TTAA 9 cut(s) 903, 1706, 2648, 3017, 3051, 3161, 3402, 3554, 3877
Sau96I GGNCC 4 cut(s) 261, 916, 1022, 1567
SchI GAGTC 4 cut(s) 962, 1994, 2335, 2566
ScrFI CCNGG 5 cut(s) 1413, 1565, 1655, 2225, 2991
SduI GDGCHC 6 cut(s) 51, 1245, 1564, 1847, 3116, 3207
SexAI ACCWGGT 1 cut(s) 2223
SfaNI GCATC 7 cut(s) 652, 775, 1447, 1789, 1835, 2754, 3320
SfcI CTRYAG 3 cut(s) 1593, 1607, 3318
Sfr274I CTCGAG 2 cut(s) 3115, 3413
SfuI TTCGAA 3 cut(s) 272, 536, 2636
SinI GGWCC 3 cut(s) 916, 1022, 1567
SlaI CTCGAG 2 cut(s) 3115, 3413
SmiMI CAYNNNNRTG 3 cut(s) 1797, 2467, 3518
SmlI CTYRAG 6 cut(s) 53, 1688, 3008, 3050, 3115, 3413
SmoI CTYRAG 6 cut(s) 53, 1688, 3008, 3050, 3115, 3413
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 7 cut(s) 5, 145, 521, 858, 872, 1935, 3652
SspI AATATT 1 cut(s) 2815
SspMI CTAG 7 cut(s) 1037, 1778, 1980, 2576, 2936, 3326, 3861
SstI GAGCTC 1 cut(s) 1847
StuI AGGCCT 1 cut(s) 172
StyD4I CCNGG 5 cut(s) 1411, 1563, 1653, 2223, 2989
StyI CCWWGG 2 cut(s) 2089, 2752
TaiI ACGT 3 cut(s) 764, 813, 1888
TatI WGTACW 3 cut(s) 513, 2969, 3588
Tru1I TTAA 9 cut(s) 903, 1706, 2648, 3017, 3051, 3161, 3402, 3554, 3877
Tru9I TTAA 9 cut(s) 903, 1706, 2648, 3017, 3051, 3161, 3402, 3554, 3877
TscAI CASTG 8 cut(s) 319, 987, 1007, 1440, 2131, 2275, 2890, 3116
TseFI GTSAC 9 cut(s) 301, 568, 874, 1149, 1732, 2230, 2858, 2887, 3169
Tsp45I GTSAC 9 cut(s) 301, 568, 874, 1149, 1732, 2230, 2858, 2887, 3169
TspGWI ACGGA 3 cut(s) 735, 1398, 3710
TspRI CASTG 8 cut(s) 319, 987, 1007, 1440, 2131, 2275, 2890, 3116
Van91I CCANNNNNTGG 1 cut(s) 1760
Vha464I CTTAAG 1 cut(s) 3050
VneI GTGCAC 1 cut(s) 1241
VpaK11BI GGWCC 3 cut(s) 916, 1022, 1567
XapI RAATTY 6 cut(s) 208, 1475, 1679, 2644, 2670, 2941
XbaI TCTAGA 2 cut(s) 1777, 1979
XceI RCATGY 2 cut(s) 2299, 3177
XcmI CCANNNNNNNNNTGG 1 cut(s) 1950
XhoI CTCGAG 2 cut(s) 3115, 3413
XmiI GTMKAC 1 cut(s) 813
XmnI GAANNNNTTC 3 cut(s) 1683, 1863, 3509
XspI CTAG 7 cut(s) 1037, 1778, 1980, 2576, 2936, 3326, 3861
ZraI GACGTC 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.