MD15G1277400.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000039
Physical Location & Seq
Forward (+)
24418981 .. 24422034
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1277400.v1.1.491

Sequence Viewer

Length: 2406 bp
ATGGGTGCTCTGAGAACGTCATTTCAAATGCTGTTTGATGCTGTAAAATCAGCGCAGGAGGAGAATACAATGTTCCAACACCTACTCGGAAACATCAAATCCACACTGGAATCTCTACAACCACTTATCAAAGACATCGAAAAGTATAACTCCAAGGAGGGGCGGGAAAATTATGCAATACAGATGGAGGAAGGAGCAAAGCTTGTTCAAAAGTGCAGCAAAGTTGGCGTGTGGAGAAAACACACATACACCAAGAAGCTTAATCAACTGGACAAATCTCTTCGAAGAGTGTTTGATGTGCTTAAACTGCAGGTTATGAGGGATGCGAGGGAGACGTTGGTTCCTTTGAGGAATATCGAGACGGTGCTCTCTCGAATAGAAGAGAGATTGAAGATAAAAAATAACCAGCCTGAAATGGTTAAATGTTCGTCTGCGGTGCCTGAAGCGCCATCGGTTACAGTCGGGTTGGATTTGCCGTTGAAGGAGTTGAAGGTGAAGCTCCTTAAGGATGAAAAGGTGTCTATGCTTGTGCTGACCGCTGCTGGAGGATGCGGGAAAACCACCTTGGCTAAAATGTTTTGTCAAGATCAAGAAGTCAAAGATACATTCAAGAACAACATCTTCTTTGTCAATGTCTCGAAAAAACCCAACTTGGACCTTATTGTACACGAACTATATCAACGGAAGGGGTCCGAGCTGCCTGCTTTCGAAAACGAAGACATGGCAGCTAACTGGTTGCAACAATTTCTGATGGAATCAAGACAAAATCCTCTACTGTTTGTCCTGGATGATGTTTGGTCAGGATCAGAGTCCCTTCTTGAGAAGTTTGATCAATTCAAGTTTTCAAATTACAAGATTTTGGTCACATCAAGATTTGCATTTCCAAGATTCGGTTCTCCTTATCATTTGGCACCATTGAATGATGAAGATGCAATGGCTCTTTTTCACTATTCTGCATCCTTGGAAGATAAGAGCTCTTATACTTACGAAGATCTTTCGAGAAAGATTATCAAGCGCTGTAAGGGATGCCCACTTGCCATTGCATTGGTTGGGAGATCACTTCGCGGCCAGCCTATAGAAATCTGGCAGAAAAGAGTAGTGGAATGGTGCAAAGGTTCATCTATTCTTGATTCTGACAAGGATTTACTTGCTTGCCTCCAAAGCAGCTTAGATGCCTTGGATAAGGAGAAGGTTATCATCAAGGAATGTTTCCTCGATCTAGGTTCTTTTCCGGAGGACCAAAGAATTGCTGCTGCCGCTCTCATTGATATGTGGGCGCAGTTATATGGTCTAGATGAAGAAATTCTGACCATTGCCAACCTTCACGAGCTCACCGCTCGAAGTTTAGCTAATCTTGTTGTCACAAGACATGAGAGGGAGACAGATGGCTATTACACTGAACACTTTGTCACCCAGCACGACATGCTTAGAGAGTTGGCGATCCATCAGGCGAGTCAAGACCCAATAGAACATAGGAAACGACTATTTATAAACATATGTGGGGTCAAACTTCCTAAGTGGTTGACAGAACAAAAGTATAAAGCGATCAAGGCTCGACTATTATCTATCTCAACAGATGGAGCCTTCTCAGCAAAATGGCCAAACATGCAAGTACCCGAAGCAGAGGTTCTAGTTCTGAATTTTCAAACAAAGAACTATGCCTTACCTGAGTTTGTGGAGAAAATGGATAAACTAAAAGTTCTAATACTAAATAATTATGGTTTGTTACCTGCTGAACTAGATAATTTTGAACTACTTGGTTCGCTATCAAATCTTAAGAGAATTAGATTAGAACATATTTCAGTTCCTTCCATAAGCAAGGTCCTCAAACAGTTGAAAAGTTTGCAAAAGATATCTTTTTTCATGTGTGATATCGGCCAAGGTTTCGTCCAAATTTTAGATGCACTGCCAAATCTAGTGGAATTAAACATTGATTATTGCCATGATCTGGTGCAACTTCCTGCCAACCTATGTGACCTTGTTCACCTTAAGAAGCTTAGCATCACCAACTGTCATAAGCTATCTGCCTTGCCTGCAGACATTGGAAAGCTAGTCAATCTAGAAGTGTTGAGGCTTAGGTCATGTGCAGACTTGTTAGAGTTGCCAGGCTCGATTAGGAACCTTGAAGTTTTGAAATTTCTCGACATATCCGATTGCTTTAGCATAAAGGAGTTGCCCGAGGACATTGGTGCAATGTGCAGCATGGAAAAGATCAACGTGAGCCAGTGTTCAAGACTGAGAGAGCTGCCTGCTTCTGTTATGGATCTTGAGCAGTTGAACGAAGTGATATGCGACGAGGAGACGAAAACCTTATGGGAACCCTTCTCGCCATTTCTCACAAACATGAGCGTAAAGGTGATCAAAGAAGATATCAACCTAAATTGGCTCAACAAGCTTCCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

802

Amino Acids

91.36

Weight (kDa)

6.28

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 123 3e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 160 - 291 2e-13 NB-ARC domain
AAA_22 PF13401 174 - 275 8.2e-07 AAA domain
LRR_14 PF23598 655 - 718 1.3e-08 Leucine-rich repeat region
LRR_14 PF23598 704 - 769 2.3e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1490
Acc36I ACCTGC 2 cut(s) 301, 1738
AccB1I GGYRCC 2 cut(s) 436, 910
AccB7I CCANNNNNTGG 1 cut(s) 1948
AccBSI CCGCTC 2 cut(s) 1259, 1337
AccII CGCG 1 cut(s) 1065
AccIII TCCGGA 1 cut(s) 1231
AciI CCGC 7 cut(s) 163, 434, 537, 552, 1065, 1257, 1335
AclWI GGATC 3 cut(s) 811, 1435, 2271
AcoI YGGCCR 3 cut(s) 1066, 1598, 1876
AcsI RAATTY 4 cut(s) 1302, 1639, 1893, 2135
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 2 cut(s) 666, 1614
AfeI AGCGCT 1 cut(s) 1016
AfiI CCNNNNNNNGG 3 cut(s) 159, 890, 1948
AflII CTTAAG 3 cut(s) 503, 1775, 1988
AjnI CCWGG 2 cut(s) 783, 2104
AjuI GAANNNNNNNTTGG 4 cut(s) 548, 580, 877, 909
Alw21I GWGCWC 4 cut(s) 10, 369, 977, 1332
Alw26I GTCTC 5 cut(s) 326, 353, 640, 1373, 2294
AlwI GGATC 3 cut(s) 811, 1435, 2271
Ama87I CYCGRG 1 cut(s) 2177
Aor13HI TCCGGA 1 cut(s) 1231
Aor51HI AGCGCT 1 cut(s) 1016
AoxI GGCC 3 cut(s) 1066, 1598, 1876
ApeKI GCWGC 9 cut(s) 216, 539, 697, 725, 1164, 1250, 1253, 2199, 2245
ApoI RAATTY 4 cut(s) 1302, 1639, 1893, 2135
Asp700I GAANNNNTTC 1 cut(s) 1302
AspLEI GCGC 4 cut(s) 55, 448, 1017, 1279
AspS9I GGNCC 4 cut(s) 655, 690, 1237, 1822
AsuHPI GGTGA 6 cut(s) 505, 1324, 1402, 1976, 1996, 2368
AsuII TTCGAA 2 cut(s) 283, 708
AvaI CYCGRG 1 cut(s) 2177
AvaII GGWCC 4 cut(s) 655, 690, 1237, 1822
BalI TGGCCA 1 cut(s) 1600
BanI GGYRCC 2 cut(s) 436, 910
BanII GRGCYC 2 cut(s) 977, 1332
BauI CACGAG 1 cut(s) 1325
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 4 cut(s) 10, 369, 977, 1332
BbvI GCAGC 9 cut(s) 228, 526, 684, 737, 1176, 1237, 1240, 2211, 2232
BccI CCATC 6 cut(s) 178, 457, 745, 1379, 1452, 1571
BceAI ACGGC 1 cut(s) 460
BcgI CGANNNNNNTGC 2 cut(s) 683, 717
BciT130I CCWGG 2 cut(s) 785, 2106
BclI TGATCA 2 cut(s) 829, 2358
BcoDI GTCTC 5 cut(s) 326, 353, 640, 1373, 2294
BfaI CTAG 7 cut(s) 1220, 1292, 1631, 1739, 1916, 2051, 2060
BfmI CTRYAG 3 cut(s) 308, 1074, 2034
BfoI RGCGCY 2 cut(s) 449, 1018
BfrI CTTAAG 3 cut(s) 503, 1775, 1988
BfuAI ACCTGC 2 cut(s) 301, 1738
BglII AGATCT 1 cut(s) 991
BlpI GCTNAGC 1 cut(s) 1997
Bme1390I CCNGG 2 cut(s) 785, 2106
Bme18I GGWCC 4 cut(s) 655, 690, 1237, 1822
BmeT110I CYCGRG 1 cut(s) 2177
BmgT120I GGNCC 4 cut(s) 655, 690, 1237, 1822
BmiI GGNNCC 7 cut(s) 342, 438, 691, 912, 1582, 2120, 2319
BmrFI CCNGG 2 cut(s) 785, 2106
BmsI GCATC 9 cut(s) 28, 313, 539, 919, 965, 1016, 1162, 1891, 2010
BpiI GAAGAC 1 cut(s) 723
BpmI CTGGAG 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 2075
Bpu1102I GCTNAGC 1 cut(s) 1997
Bpu14I TTCGAA 2 cut(s) 283, 708
BpuEI CTTGAG 2 cut(s) 839, 2288
BsaJI CCNNGG 6 cut(s) 153, 564, 960, 1176, 1879, 2178
BsaWI WCCGGW 1 cut(s) 1231
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 890, 1948
Bse1I ACTGG 4 cut(s) 111, 273, 737, 2224
Bse3DI GCAATG 4 cut(s) 939, 1038, 1311, 2199
BseAI TCCGGA 1 cut(s) 1231
BseBI CCWGG 2 cut(s) 785, 2106
BseDI CCNNGG 6 cut(s) 153, 564, 960, 1176, 1879, 2178
BseGI GGATG 7 cut(s) 328, 514, 554, 793, 956, 1031, 2399
BseLI CCNNNNNNNGG 3 cut(s) 159, 890, 1948
BseMI GCAATG 4 cut(s) 939, 1038, 1311, 2199
BseMII CTCAG 3 cut(s) 1602, 1659, 2228
BseNI ACTGG 4 cut(s) 111, 273, 737, 2224
BseRI GAGGAG 2 cut(s) 74, 2312
BseXI GCAGC 9 cut(s) 228, 526, 684, 737, 1176, 1237, 1240, 2211, 2232
BseYI CCCAGC 1 cut(s) 1413
BsgI GTGCAG 3 cut(s) 235, 2106, 2218
Bsh1236I CGCG 1 cut(s) 1065
BshFI GGCC 3 cut(s) 1068, 1600, 1878
BshNI GGYRCC 2 cut(s) 436, 910
BsiHKAI GWGCWC 4 cut(s) 10, 369, 977, 1332
BsiHKCI CYCGRG 1 cut(s) 2177
BsiSI CCGG 1 cut(s) 1232
BslFI GGGAC 1 cut(s) 796
BslI CCNNNNNNNGG 3 cut(s) 159, 890, 1948
BsmAI GTCTC 5 cut(s) 326, 353, 640, 1373, 2294
BsmBI CGTCTC 3 cut(s) 326, 353, 2294
BsmFI GGGAC 1 cut(s) 796
BsnI GGCC 3 cut(s) 1068, 1600, 1878
BsoBI CYCGRG 1 cut(s) 2177
Bsp119I TTCGAA 2 cut(s) 283, 708
Bsp1286I GDGCHC 4 cut(s) 10, 369, 977, 1332
Bsp13I TCCGGA 1 cut(s) 1231
Bsp1407I TGTACA 1 cut(s) 664
Bsp1720I GCTNAGC 1 cut(s) 1997
BspACI CCGC 7 cut(s) 163, 434, 537, 552, 1065, 1257, 1335
BspANI GGCC 3 cut(s) 1068, 1600, 1878
BspCNI CTCAG 3 cut(s) 1601, 1660, 2229
BspEI TCCGGA 1 cut(s) 1231
BspFNI CGCG 1 cut(s) 1065
BspLI GGNNCC 7 cut(s) 342, 438, 691, 912, 1582, 2120, 2319
BspMAI CTGCAG 2 cut(s) 312, 2038
BspMI ACCTGC 2 cut(s) 301, 1738
BspPI GGATC 3 cut(s) 811, 1435, 2271
BspT104I TTCGAA 2 cut(s) 283, 708
BspT107I GGYRCC 2 cut(s) 436, 910
BspTI CTTAAG 3 cut(s) 503, 1775, 1988
BsrBI CCGCTC 2 cut(s) 1259, 1337
BsrDI GCAATG 4 cut(s) 939, 1038, 1311, 2199
BsrGI TGTACA 1 cut(s) 664
BsrI ACTGG 4 cut(s) 111, 273, 737, 2224
BssECI CCNNGG 6 cut(s) 153, 564, 960, 1176, 1879, 2178
BssSI CACGAG 1 cut(s) 1325
BssT1I CCWWGG 5 cut(s) 153, 564, 960, 1176, 1879
Bst2BI CACGAG 1 cut(s) 1325
Bst2UI CCWGG 2 cut(s) 785, 2106
Bst4CI ACNGT 5 cut(s) 364, 460, 777, 1833, 2012
Bst6I CTCTTC 3 cut(s) 280, 285, 375
BstAFI CTTAAG 3 cut(s) 503, 1775, 1988
BstAPI GCANNNNNTGC 1 cut(s) 1423
BstAUI TGTACA 1 cut(s) 664
BstBI TTCGAA 2 cut(s) 283, 708
BstC8I GCNNGC 5 cut(s) 702, 1070, 1153, 2034, 2250
BstDEI CTNAG 9 cut(s) 11, 1168, 1427, 1515, 1588, 1668, 1997, 2075, 2237
BstF5I GGATG 7 cut(s) 328, 514, 554, 793, 956, 1031, 2399
BstFNI CGCG 1 cut(s) 1065
BstH2I RGCGCY 2 cut(s) 449, 1018
BstHHI GCGC 4 cut(s) 55, 448, 1017, 1279
BstMAI GTCTC 5 cut(s) 326, 353, 640, 1373, 2294
BstNI CCWGG 2 cut(s) 785, 2106
BstNSI RCATGY 2 cut(s) 1426, 1609
BstSCI CCNGG 2 cut(s) 783, 2104
BstSFI CTRYAG 3 cut(s) 308, 1074, 2034
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 9 cut(s) 228, 526, 684, 737, 1176, 1237, 1240, 2211, 2232
BstV2I GAAGAC 1 cut(s) 723
BstX2I RGATCY 2 cut(s) 991, 2263
BstXI CCANNNNNNTGG 1 cut(s) 1045
BstYI RGATCY 2 cut(s) 991, 2263
BsuRI GGCC 3 cut(s) 1068, 1600, 1878
BtsCI GGATG 7 cut(s) 328, 514, 554, 793, 956, 1031, 2399
BtsI GCAGTG 1 cut(s) 1904
BtsIMutI CAGTG 4 cut(s) 104, 1395, 1904, 2231
BveI ACCTGC 2 cut(s) 301, 1738
Cac8I GCNNGC 5 cut(s) 702, 1070, 1153, 2034, 2250
CfoI GCGC 4 cut(s) 55, 448, 1017, 1279
Cfr13I GGNCC 4 cut(s) 655, 690, 1237, 1822
Csp6I GTAC 2 cut(s) 665, 1613
CspCI CAANNNNNGTGG 2 cut(s) 1899, 1934
CviAII CATG 9 cut(s) 721, 1370, 1423, 1606, 1864, 1943, 2082, 2203, 2344
CviQI GTAC 2 cut(s) 665, 1613
DdeI CTNAG 9 cut(s) 11, 1168, 1427, 1515, 1588, 1668, 1997, 2075, 2237
EaeI YGGCCR 3 cut(s) 1066, 1598, 1876
Eam1104I CTCTTC 3 cut(s) 280, 285, 375
EarI CTCTTC 3 cut(s) 280, 285, 375
Ecl136II GAGCTC 2 cut(s) 975, 1330
Eco130I CCWWGG 5 cut(s) 153, 564, 960, 1176, 1879
Eco24I GRGCYC 2 cut(s) 977, 1332
Eco32I GATATC 3 cut(s) 1854, 1873, 2371
Eco47I GGWCC 4 cut(s) 655, 690, 1237, 1822
Eco47III AGCGCT 1 cut(s) 1016
Eco53kI GAGCTC 2 cut(s) 975, 1330
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 2177
EcoICRI GAGCTC 2 cut(s) 975, 1330
EcoO109I RGGNCCY 1 cut(s) 1822
EcoRII CCWGG 2 cut(s) 783, 2104
EcoRV GATATC 3 cut(s) 1854, 1873, 2371
EcoT14I CCWWGG 5 cut(s) 153, 564, 960, 1176, 1879
EcoT38I GRGCYC 2 cut(s) 977, 1332
ErhI CCWWGG 5 cut(s) 153, 564, 960, 1176, 1879
Esp3I CGTCTC 3 cut(s) 326, 353, 2294
FaeI CATG 9 cut(s) 724, 1373, 1426, 1609, 1867, 1946, 2085, 2206, 2347
FaqI GGGAC 1 cut(s) 796
FatI CATG 9 cut(s) 720, 1369, 1422, 1605, 1863, 1942, 2081, 2202, 2343
FauI CCCGC 2 cut(s) 156, 545
FauNDI CATATG 1 cut(s) 1496
FbaI TGATCA 2 cut(s) 829, 2358
FokI GGATG 7 cut(s) 335, 521, 561, 800, 943, 1038, 2386
FriOI GRGCYC 2 cut(s) 977, 1332
FspBI CTAG 7 cut(s) 1220, 1292, 1631, 1739, 1916, 2051, 2060
GlaI GCGC 4 cut(s) 54, 447, 1016, 1278
GsaI CCCAGC 1 cut(s) 1417
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 2 cut(s) 449, 1018
HaeIII GGCC 3 cut(s) 1068, 1600, 1878
HapII CCGG 1 cut(s) 1232
HhaI GCGC 4 cut(s) 55, 448, 1017, 1279
Hin1II CATG 9 cut(s) 724, 1373, 1426, 1609, 1867, 1946, 2085, 2206, 2347
Hin6I GCGC 4 cut(s) 53, 446, 1015, 1277
HinP1I GCGC 4 cut(s) 53, 446, 1015, 1277
HincII GTYRAC 1 cut(s) 1524
HindII GTYRAC 1 cut(s) 1524
HindIII AAGCTT 4 cut(s) 200, 257, 1994, 2393
HinfI GANTC 6 cut(s) 110, 755, 809, 888, 1130, 1453
HpaII CCGG 1 cut(s) 1232
HphI GGTGA 6 cut(s) 505, 1324, 1402, 1976, 1996, 2368
Hpy166II GTNNAC 3 cut(s) 667, 1524, 1984
Hpy188I TCNGA 9 cut(s) 12, 89, 694, 750, 808, 1135, 1308, 1638, 2152
Hpy8I GTNNAC 3 cut(s) 667, 1524, 1984
Hpy99I CGWCG 1 cut(s) 2297
HpyCH4III ACNGT 5 cut(s) 364, 460, 777, 1833, 2012
HpyCH4IV ACGT 3 cut(s) 17, 335, 2217
HpyF3I CTNAG 9 cut(s) 11, 1168, 1427, 1515, 1588, 1668, 1997, 2075, 2237
HpySE526I ACGT 3 cut(s) 17, 335, 2217
Hsp92II CATG 9 cut(s) 724, 1373, 1426, 1609, 1867, 1946, 2085, 2206, 2347
HspAI GCGC 4 cut(s) 53, 446, 1015, 1277
Kpn2I TCCGGA 1 cut(s) 1231
Ksp22I TGATCA 2 cut(s) 829, 2358
LmnI GCTCC 3 cut(s) 194, 504, 1580
Lsp1109I GCAGC 9 cut(s) 228, 526, 684, 737, 1176, 1237, 1240, 2211, 2232
LweI GCATC 9 cut(s) 28, 313, 539, 919, 965, 1016, 1162, 1891, 2010
MaeI CTAG 7 cut(s) 1220, 1292, 1631, 1739, 1916, 2051, 2060
MaeII ACGT 3 cut(s) 17, 335, 2217
MaeIII GTNAC 6 cut(s) 454, 862, 1360, 1408, 1725, 1973
MbiI CCGCTC 2 cut(s) 1259, 1337
MflI RGATCY 2 cut(s) 991, 2263
MhlI GDGCHC 4 cut(s) 10, 369, 977, 1332
MlsI TGGCCA 1 cut(s) 1600
MluNI TGGCCA 1 cut(s) 1600
MlyI GAGTC 2 cut(s) 818, 1462
MmeI TCCRAC 2 cut(s) 100, 447
Mox20I TGGCCA 1 cut(s) 1600
MroI TCCGGA 1 cut(s) 1231
MroXI GAANNNNTTC 1 cut(s) 1302
MscI TGGCCA 1 cut(s) 1600
MseI TTAA 7 cut(s) 261, 303, 420, 504, 1776, 1925, 1989
MslI CAYNNNNRTG 2 cut(s) 1268, 2342
Msp20I TGGCCA 1 cut(s) 1600
MspA1I CMGCKG 1 cut(s) 539
MspCI CTTAAG 3 cut(s) 503, 1775, 1988
MspI CCGG 1 cut(s) 1232
MspR9I CCNGG 2 cut(s) 785, 2106
MvaI CCWGG 2 cut(s) 785, 2106
MvnI CGCG 1 cut(s) 1065
NdeI CATATG 1 cut(s) 1496
NlaIII CATG 9 cut(s) 724, 1373, 1426, 1609, 1867, 1946, 2085, 2206, 2347
NlaIV GGNNCC 7 cut(s) 342, 438, 691, 912, 1582, 2120, 2319
NmuCI GTSAC 4 cut(s) 862, 1360, 1408, 1973
NspI RCATGY 2 cut(s) 1426, 1609
NspV TTCGAA 2 cut(s) 283, 708
PcsI WCGNNNNNNNCGW 1 cut(s) 2148
PdmI GAANNNNTTC 1 cut(s) 1302
PfeI GAWTC 4 cut(s) 110, 755, 888, 1130
PflMI CCANNNNNTGG 1 cut(s) 1948
PfoI TCCNGGA 1 cut(s) 783
PleI GAGTC 2 cut(s) 817, 1461
PpsI GAGTC 2 cut(s) 817, 1461
PpuMI RGGWCCY 1 cut(s) 1822
PsiI TTATAA 1 cut(s) 1490
Psp124BI GAGCTC 2 cut(s) 977, 1332
Psp5II RGGWCCY 1 cut(s) 1822
Psp6I CCWGG 2 cut(s) 783, 2104
PspFI CCCAGC 1 cut(s) 1413
PspGI CCWGG 2 cut(s) 783, 2104
PspN4I GGNNCC 7 cut(s) 342, 438, 691, 912, 1582, 2120, 2319
PspPI GGNCC 4 cut(s) 655, 690, 1237, 1822
PspPPI RGGWCCY 1 cut(s) 1822
PstI CTGCAG 2 cut(s) 312, 2038
PsuI RGATCY 2 cut(s) 991, 2263
RsaI GTAC 2 cut(s) 666, 1614
RsaNI GTAC 2 cut(s) 665, 1613
RseI CAYNNNNRTG 2 cut(s) 1268, 2342
SacI GAGCTC 2 cut(s) 977, 1332
SaqAI TTAA 7 cut(s) 261, 303, 420, 504, 1776, 1925, 1989
Sau96I GGNCC 4 cut(s) 655, 690, 1237, 1822
SchI GAGTC 2 cut(s) 818, 1462
ScrFI CCNGG 2 cut(s) 785, 2106
SduI GDGCHC 4 cut(s) 10, 369, 977, 1332
SfaNI GCATC 9 cut(s) 28, 313, 539, 919, 965, 1016, 1162, 1891, 2010
SfcI CTRYAG 3 cut(s) 308, 1074, 2034
SfuI TTCGAA 2 cut(s) 283, 708
SinI GGWCC 4 cut(s) 655, 690, 1237, 1822
SmiMI CAYNNNNRTG 2 cut(s) 1268, 2342
SmlI CTYRAG 5 cut(s) 503, 818, 1775, 1988, 2267
SmoI CTYRAG 5 cut(s) 503, 818, 1775, 1988, 2267
SsiI CCGC 7 cut(s) 163, 434, 537, 552, 1065, 1257, 1335
SspMI CTAG 7 cut(s) 1220, 1292, 1631, 1739, 1916, 2051, 2060
SstI GAGCTC 2 cut(s) 977, 1332
StyD4I CCNGG 2 cut(s) 783, 2104
StyI CCWWGG 5 cut(s) 153, 564, 960, 1176, 1879
TaaI ACNGT 5 cut(s) 364, 460, 777, 1833, 2012
TaiI ACGT 3 cut(s) 20, 338, 2220
TatI WGTACW 1 cut(s) 664
TauI GCSGC 2 cut(s) 1068, 1259
TfiI GAWTC 4 cut(s) 110, 755, 888, 1130
Tru1I TTAA 7 cut(s) 261, 303, 420, 504, 1776, 1925, 1989
Tru9I TTAA 7 cut(s) 261, 303, 420, 504, 1776, 1925, 1989
TscAI CASTG 4 cut(s) 111, 1402, 1911, 2231
TseFI GTSAC 4 cut(s) 862, 1360, 1408, 1973
TseI GCWGC 9 cut(s) 216, 539, 697, 725, 1164, 1250, 1253, 2199, 2245
Tsp45I GTSAC 4 cut(s) 862, 1360, 1408, 1973
TspDTI ATGAA 5 cut(s) 525, 939, 1107, 1311, 1852
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 4 cut(s) 111, 1402, 1911, 2231
Van91I CCANNNNNTGG 1 cut(s) 1948
Vha464I CTTAAG 3 cut(s) 503, 1775, 1988
VpaK11BI GGWCC 4 cut(s) 655, 690, 1237, 1822
XapI RAATTY 4 cut(s) 1302, 1639, 1893, 2135
XbaI TCTAGA 2 cut(s) 1291, 2059
XceI RCATGY 2 cut(s) 1426, 1609
XmnI GAANNNNTTC 1 cut(s) 1302
XspI CTAG 7 cut(s) 1220, 1292, 1631, 1739, 1916, 2051, 2060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.